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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 792 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 98, 131, 487 |
| AccBSI | CCGCTC | 1 cut(s) | 779 |
| AccI | GTMKAC | 1 cut(s) | 1419 |
| AccII | CGCG | 9 cut(s) | 108, 785, 852, 993, 1112, 1205, 1313, 1357, 1415 |
| AccIII | TCCGGA | 1 cut(s) | 1258 |
| AclWI | GGATC | 8 cut(s) | 24, 245, 258, 397, 521, 773, 1329, 1342 |
| AcoI | YGGCCR | 2 cut(s) | 133, 780 |
| AcuI | CTGAAG | 3 cut(s) | 192, 456, 612 |
| AfaI | GTAC | 3 cut(s) | 573, 680, 1186 |
| AflIII | ACRYGT | 1 cut(s) | 1110 |
| AgeI | ACCGGT | 1 cut(s) | 22 |
| AgsI | TTSAA | 9 cut(s) | 179, 578, 887, 962, 1091, 1119, 1150, 1181, 1191 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 912 |
| AjiI | CACGTC | 1 cut(s) | 38 |
| AjnI | CCWGG | 4 cut(s) | 45, 411, 608, 1457 |
| AluBI | AGCT | 8 cut(s) | 128, 315, 503, 671, 797, 806, 983, 1019 |
| AluI | AGCT | 8 cut(s) | 128, 315, 503, 671, 797, 806, 983, 1019 |
| Alw21I | GWGCWC | 1 cut(s) | 673 |
| Alw26I | GTCTC | 5 cut(s) | 410, 710, 920, 975, 1252 |
| AlwI | GGATC | 8 cut(s) | 24, 245, 258, 397, 521, 773, 1329, 1342 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 131 |
| Ama87I | CYCGRG | 1 cut(s) | 917 |
| Aor13HI | TCCGGA | 1 cut(s) | 1258 |
| ApeKI | GCWGC | 5 cut(s) | 512, 515, 518, 1019, 1022 |
| AsiGI | ACCGGT | 1 cut(s) | 22 |
| AspLEI | GCGC | 4 cut(s) | 110, 240, 400, 793 |
| AspS9I | GGNCC | 7 cut(s) | 43, 478, 583, 823, 1000, 1227, 1430 |
| AsuC2I | CCSGG | 2 cut(s) | 654, 1427 |
| AsuHPI | GGTGA | 5 cut(s) | 169, 364, 463, 718, 916 |
| AvaI | CYCGRG | 1 cut(s) | 917 |
| AvaII | GGWCC | 2 cut(s) | 1227, 1430 |
| AxyI | CCTNAGG | 1 cut(s) | 360 |
| BalI | TGGCCA | 1 cut(s) | 135 |
| BamHI | GGATCC | 2 cut(s) | 250, 1334 |
| BanII | GRGCYC | 2 cut(s) | 621, 673 |
| BbsI | GAAGAC | 3 cut(s) | 620, 1098, 1389 |
| Bbv12I | GWGCWC | 1 cut(s) | 673 |
| BbvI | GCAGC | 5 cut(s) | 499, 502, 505, 1006, 1009 |
| BccI | CCATC | 4 cut(s) | 608, 716, 1166, 1206 |
| BceAI | ACGGC | 1 cut(s) | 924 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1278, 1312 |
| BciT130I | CCWGG | 4 cut(s) | 47, 413, 610, 1459 |
| BcnI | CCSGG | 2 cut(s) | 654, 1427 |
| BcoDI | GTCTC | 5 cut(s) | 410, 710, 920, 975, 1252 |
| BfaI | CTAG | 6 cut(s) | 563, 798, 938, 980, 1032, 1142 |
| BfmI | CTRYAG | 2 cut(s) | 746, 1023 |
| BmcAI | AGTACT | 2 cut(s) | 680, 1186 |
| Bme1390I | CCNGG | 6 cut(s) | 47, 413, 610, 654, 1427, 1459 |
| Bme18I | GGWCC | 2 cut(s) | 1227, 1430 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 912 |
| BmeT110I | CYCGRG | 1 cut(s) | 917 |
| BmgBI | CACGTC | 1 cut(s) | 38 |
| BmgT120I | GGNCC | 7 cut(s) | 43, 478, 583, 823, 1000, 1227, 1430 |
| BmrFI | CCNGG | 6 cut(s) | 47, 413, 610, 654, 1427, 1459 |
| BmsI | GCATC | 2 cut(s) | 668, 1018 |
| BpiI | GAAGAC | 3 cut(s) | 620, 1098, 1389 |
| BpmI | CTGGAG | 3 cut(s) | 108, 507, 695 |
| Bpu10I | CCTNAGC | 1 cut(s) | 984 |
| BpuEI | CTTGAG | 1 cut(s) | 792 |
| BpuMI | CCSGG | 2 cut(s) | 654, 1427 |
| Bsa29I | ATCGAT | 1 cut(s) | 295 |
| BsaAI | YACGTR | 1 cut(s) | 571 |
| BsaBI | GATNNNNATC | 1 cut(s) | 201 |
| BsaI | GGTCTC | 1 cut(s) | 1252 |
| BsaJI | CCNNGG | 8 cut(s) | 45, 78, 225, 266, 609, 1413, 1426, 1457 |
| BsaWI | WCCGGW | 4 cut(s) | 22, 463, 1229, 1258 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 435, 465 |
| Bse118I | RCCGGY | 2 cut(s) | 22, 1272 |
| Bse1I | ACTGG | 3 cut(s) | 136, 136, 558 |
| Bse21I | CCTNAGG | 1 cut(s) | 360 |
| Bse8I | GATNNNNATC | 1 cut(s) | 201 |
| BseAI | TCCGGA | 1 cut(s) | 1258 |
| BseBI | CCWGG | 4 cut(s) | 47, 413, 610, 1459 |
| BseCI | ATCGAT | 1 cut(s) | 295 |
| BseDI | CCNNGG | 8 cut(s) | 45, 78, 225, 266, 609, 1413, 1426, 1457 |
| BseGI | GGATG | 5 cut(s) | 379, 619, 642, 659, 1267 |
| BseJI | GATNNNNATC | 1 cut(s) | 201 |
| BseMII | CTCAG | 6 cut(s) | 351, 623, 705, 711, 998, 1237 |
| BseNI | ACTGG | 3 cut(s) | 136, 136, 558 |
| BseRI | GAGGAG | 1 cut(s) | 179 |
| BseX3I | CGGCCG | 1 cut(s) | 780 |
| BseXI | GCAGC | 5 cut(s) | 499, 502, 505, 1006, 1009 |
| Bsh1236I | CGCG | 9 cut(s) | 108, 785, 852, 993, 1112, 1205, 1313, 1357, 1415 |
| Bsh1285I | CGRYCG | 1 cut(s) | 783 |
| BshTI | ACCGGT | 1 cut(s) | 22 |
| BshVI | ATCGAT | 1 cut(s) | 295 |
| BsiEI | CGRYCG | 1 cut(s) | 783 |
| BsiHKAI | GWGCWC | 1 cut(s) | 673 |
| BsiHKCI | CYCGRG | 1 cut(s) | 917 |
| BsiSI | CCGG | 8 cut(s) | 23, 464, 470, 653, 1230, 1259, 1273, 1426 |
| BslFI | GGGAC | 1 cut(s) | 621 |
| BsmAI | GTCTC | 5 cut(s) | 410, 710, 920, 975, 1252 |
| BsmFI | GGGAC | 1 cut(s) | 621 |
| BsmI | GAATGC | 2 cut(s) | 460, 1145 |
| Bso31I | GGTCTC | 1 cut(s) | 1252 |
| BsoBI | CYCGRG | 1 cut(s) | 917 |
| Bsp1286I | GDGCHC | 2 cut(s) | 621, 673 |
| Bsp13I | TCCGGA | 1 cut(s) | 1258 |
| Bsp143I | GATC | 8 cut(s) | 29, 159, 250, 292, 402, 526, 765, 1334 |
| Bsp19I | CCATGG | 1 cut(s) | 266 |
| Bsp68I | TCGCGA | 2 cut(s) | 852, 993 |
| BspCNI | CTCAG | 6 cut(s) | 352, 624, 706, 712, 997, 1236 |
| BspDI | ATCGAT | 1 cut(s) | 295 |
| BspEI | TCCGGA | 1 cut(s) | 1258 |
| BspFNI | CGCG | 9 cut(s) | 108, 785, 852, 993, 1112, 1205, 1313, 1357, 1415 |
| BspHI | TCATGA | 1 cut(s) | 496 |
| BspMAI | CTGCAG | 1 cut(s) | 1027 |
| BspPI | GGATC | 8 cut(s) | 24, 245, 258, 397, 521, 773, 1329, 1342 |
| BspTNI | GGTCTC | 1 cut(s) | 1252 |
| BsrBI | CCGCTC | 1 cut(s) | 779 |
| BsrFI | RCCGGY | 2 cut(s) | 22, 1272 |
| BsrI | ACTGG | 3 cut(s) | 136, 136, 558 |
| BssAI | RCCGGY | 2 cut(s) | 22, 1272 |
| BssECI | CCNNGG | 8 cut(s) | 45, 78, 225, 266, 609, 1413, 1426, 1457 |
| BssMI | GATC | 8 cut(s) | 29, 159, 250, 292, 402, 526, 765, 1334 |
| BssT1I | CCWWGG | 2 cut(s) | 225, 266 |
| Bst2UI | CCWGG | 4 cut(s) | 47, 413, 610, 1459 |
| Bst4CI | ACNGT | 2 cut(s) | 157, 214 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 116 |
| BstBAI | YACGTR | 1 cut(s) | 571 |
| BstC8I | GCNNGC | 7 cut(s) | 68, 232, 236, 795, 804, 991, 1017 |
| BstDEI | CTNAG | 6 cut(s) | 360, 632, 714, 720, 984, 1223 |
| BstDSI | CCRYGG | 2 cut(s) | 266, 1413 |
| BstEII | GGTNACC | 1 cut(s) | 1079 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 983 |
| BstF5I | GGATG | 5 cut(s) | 379, 619, 642, 659, 1267 |
| BstFNI | CGCG | 9 cut(s) | 108, 785, 852, 993, 1112, 1205, 1313, 1357, 1415 |
| BstHHI | GCGC | 4 cut(s) | 110, 240, 400, 793 |
| BstKTI | GATC | 8 cut(s) | 32, 162, 253, 295, 405, 529, 768, 1337 |
| BstMAI | GTCTC | 5 cut(s) | 410, 710, 920, 975, 1252 |
| BstMBI | GATC | 8 cut(s) | 29, 159, 250, 292, 402, 526, 765, 1334 |
| BstMCI | CGRYCG | 1 cut(s) | 783 |
| BstNI | CCWGG | 4 cut(s) | 47, 413, 610, 1459 |
| BstNSI | RCATGY | 2 cut(s) | 1240, 1299 |
| BstPI | GGTNACC | 1 cut(s) | 1079 |
| BstSCI | CCNGG | 6 cut(s) | 45, 411, 608, 652, 1425, 1457 |
| BstSFI | CTRYAG | 2 cut(s) | 746, 1023 |
| BstUI | CGCG | 9 cut(s) | 108, 785, 852, 993, 1112, 1205, 1313, 1357, 1415 |
| BstV1I | GCAGC | 5 cut(s) | 499, 502, 505, 1006, 1009 |
| BstV2I | GAAGAC | 3 cut(s) | 620, 1098, 1389 |
| BstX2I | RGATCY | 4 cut(s) | 250, 402, 526, 1334 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 226, 673 |
| BstYI | RGATCY | 4 cut(s) | 250, 402, 526, 1334 |
| BstZI | CGGCCG | 1 cut(s) | 780 |
| Bsu15I | ATCGAT | 1 cut(s) | 295 |
| Bsu36I | CCTNAGG | 1 cut(s) | 360 |
| BsuTUI | ATCGAT | 1 cut(s) | 295 |
| BtgI | CCRYGG | 2 cut(s) | 266, 1413 |
| BtrI | CACGTC | 1 cut(s) | 38 |
| BtsCI | GGATG | 5 cut(s) | 379, 619, 642, 659, 1267 |
| BtsI | GCAGTG | 2 cut(s) | 219, 530 |
| BtsIMutI | CAGTG | 3 cut(s) | 219, 530, 711 |
| BtuMI | TCGCGA | 2 cut(s) | 852, 993 |
| Cac8I | GCNNGC | 7 cut(s) | 68, 232, 236, 795, 804, 991, 1017 |
| CaiI | CAGNNNCTG | 1 cut(s) | 131 |
| CciI | TCATGA | 1 cut(s) | 496 |
| CciNI | GCGGCCGC | 1 cut(s) | 780 |
| CfoI | GCGC | 4 cut(s) | 110, 240, 400, 793 |
| Cfr10I | RCCGGY | 2 cut(s) | 22, 1272 |
| Cfr13I | GGNCC | 7 cut(s) | 43, 478, 583, 823, 1000, 1227, 1430 |
| Cfr42I | CCGCGG | 1 cut(s) | 1416 |
| ClaI | ATCGAT | 1 cut(s) | 295 |
| CseI | GACGC | 1 cut(s) | 359 |
| Csp6I | GTAC | 3 cut(s) | 572, 679, 1185 |
| CspAI | ACCGGT | 1 cut(s) | 22 |
| CviAII | CATG | 9 cut(s) | 267, 497, 506, 536, 923, 1100, 1212, 1237, 1296 |
| CviQI | GTAC | 3 cut(s) | 572, 679, 1185 |
| DdeI | CTNAG | 6 cut(s) | 360, 632, 714, 720, 984, 1223 |
| DpnI | GATC | 8 cut(s) | 31, 161, 252, 294, 404, 528, 767, 1336 |
| DpnII | GATC | 8 cut(s) | 29, 159, 250, 292, 402, 526, 765, 1334 |
| DriI | GACNNNNNGTC | 1 cut(s) | 912 |
| EaeI | YGGCCR | 2 cut(s) | 133, 780 |
| EagI | CGGCCG | 1 cut(s) | 780 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 912 |
| EciI | GGCGGA | 3 cut(s) | 652, 1331, 1394 |
| Ecl136II | GAGCTC | 1 cut(s) | 671 |
| EclXI | CGGCCG | 1 cut(s) | 780 |
| Eco130I | CCWWGG | 2 cut(s) | 225, 266 |
| Eco24I | GRGCYC | 2 cut(s) | 621, 673 |
| Eco31I | GGTCTC | 1 cut(s) | 1252 |
| Eco47I | GGWCC | 2 cut(s) | 1227, 1430 |
| Eco52I | CGGCCG | 1 cut(s) | 780 |
| Eco53kI | GAGCTC | 1 cut(s) | 671 |
| Eco57I | CTGAAG | 3 cut(s) | 192, 456, 612 |
| Eco81I | CCTNAGG | 1 cut(s) | 360 |
| Eco88I | CYCGRG | 1 cut(s) | 917 |
| Eco91I | GGTNACC | 1 cut(s) | 1079 |
| EcoICRI | GAGCTC | 1 cut(s) | 671 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 983 |
| EcoO109I | RGGNCCY | 1 cut(s) | 823 |
| EcoO65I | GGTNACC | 1 cut(s) | 1079 |
| EcoRII | CCWGG | 4 cut(s) | 45, 411, 608, 1457 |
| EcoT14I | CCWWGG | 2 cut(s) | 225, 266 |
| EcoT38I | GRGCYC | 2 cut(s) | 621, 673 |
| ErhI | CCWWGG | 2 cut(s) | 225, 266 |
| FaeI | CATG | 9 cut(s) | 270, 500, 509, 539, 926, 1103, 1215, 1240, 1299 |
| FaqI | GGGAC | 1 cut(s) | 621 |
| FatI | CATG | 9 cut(s) | 266, 496, 505, 535, 922, 1099, 1211, 1236, 1295 |
| FauI | CCCGC | 1 cut(s) | 433 |
| FblI | GTMKAC | 1 cut(s) | 1419 |
| FokI | GGATG | 5 cut(s) | 366, 626, 629, 646, 1274 |
| FriOI | GRGCYC | 2 cut(s) | 621, 673 |
| FspBI | CTAG | 6 cut(s) | 563, 798, 938, 980, 1032, 1142 |
| FspI | TGCGCA | 1 cut(s) | 792 |
| GlaI | GCGC | 4 cut(s) | 109, 239, 399, 792 |
| GsuI | CTGGAG | 3 cut(s) | 108, 507, 695 |
| HapII | CCGG | 8 cut(s) | 23, 464, 470, 653, 1230, 1259, 1273, 1426 |
| HgaI | GACGC | 1 cut(s) | 359 |
| HhaI | GCGC | 4 cut(s) | 110, 240, 400, 793 |
| Hin1II | CATG | 9 cut(s) | 270, 500, 509, 539, 926, 1103, 1215, 1240, 1299 |
| Hin6I | GCGC | 4 cut(s) | 108, 238, 398, 791 |
| HinP1I | GCGC | 4 cut(s) | 108, 238, 398, 791 |
| HinfI | GANTC | 4 cut(s) | 182, 634, 751, 1138 |
| HpaII | CCGG | 8 cut(s) | 23, 464, 470, 653, 1230, 1259, 1273, 1426 |
| HphI | GGTGA | 5 cut(s) | 169, 364, 463, 718, 916 |
| Hpy166II | GTNNAC | 3 cut(s) | 568, 726, 1420 |
| Hpy188I | TCNGA | 7 cut(s) | 79, 193, 250, 349, 633, 1348, 1434 |
| Hpy8I | GTNNAC | 3 cut(s) | 568, 726, 1420 |
| HpyAV | CCTTC | 6 cut(s) | 84, 105, 354, 440, 555, 1135 |
| HpyCH4III | ACNGT | 2 cut(s) | 157, 214 |
| HpyCH4IV | ACGT | 3 cut(s) | 37, 570, 625 |
| HpyCH4V | TGCA | 4 cut(s) | 535, 659, 1025, 1060 |
| HpyF3I | CTNAG | 6 cut(s) | 360, 632, 714, 720, 984, 1223 |
| HpySE526I | ACGT | 3 cut(s) | 37, 570, 625 |
| Hsp92II | CATG | 9 cut(s) | 270, 500, 509, 539, 926, 1103, 1215, 1240, 1299 |
| HspAI | GCGC | 4 cut(s) | 108, 238, 398, 791 |
| Kpn2I | TCCGGA | 1 cut(s) | 1258 |
| KspI | CCGCGG | 1 cut(s) | 1416 |
| Kzo9I | GATC | 8 cut(s) | 29, 159, 250, 292, 402, 526, 765, 1334 |
| LmnI | GCTCC | 9 cut(s) | 166, 320, 466, 490, 526, 624, 1012, 1309, 1361 |
| Lsp1109I | GCAGC | 5 cut(s) | 499, 502, 505, 1006, 1009 |
| LweI | GCATC | 2 cut(s) | 668, 1018 |
| MaeI | CTAG | 6 cut(s) | 563, 798, 938, 980, 1032, 1142 |
| MaeII | ACGT | 3 cut(s) | 37, 570, 625 |
| MaeIII | GTNAC | 3 cut(s) | 13, 904, 1079 |
| MalI | GATC | 8 cut(s) | 31, 161, 252, 294, 404, 528, 767, 1336 |
| MbiI | CCGCTC | 1 cut(s) | 779 |
| MboI | GATC | 8 cut(s) | 29, 159, 250, 292, 402, 526, 765, 1334 |
| MflI | RGATCY | 4 cut(s) | 250, 402, 526, 1334 |
| MhlI | GDGCHC | 2 cut(s) | 621, 673 |
| MlsI | TGGCCA | 1 cut(s) | 135 |
| MluCI | AATT | 8 cut(s) | 60, 323, 407, 893, 1063, 1070, 1119, 1150 |
| MluI | ACGCGT | 1 cut(s) | 1110 |
| MluNI | TGGCCA | 1 cut(s) | 135 |
| MlyI | GAGTC | 1 cut(s) | 643 |
| MmeI | TCCRAC | 2 cut(s) | 646, 1457 |
| Mox20I | TGGCCA | 1 cut(s) | 135 |
| MroI | TCCGGA | 1 cut(s) | 1258 |
| MscI | TGGCCA | 1 cut(s) | 135 |
| MseI | TTAA | 2 cut(s) | 683, 896 |
| Msp20I | TGGCCA | 1 cut(s) | 135 |
| MspA1I | CMGCKG | 2 cut(s) | 512, 1415 |
| MspI | CCGG | 8 cut(s) | 23, 464, 470, 653, 1230, 1259, 1273, 1426 |
| MspR9I | CCNGG | 6 cut(s) | 47, 413, 610, 654, 1427, 1459 |
| Mva1269I | GAATGC | 2 cut(s) | 460, 1145 |
| MvaI | CCWGG | 4 cut(s) | 47, 413, 610, 1459 |
| MvnI | CGCG | 9 cut(s) | 108, 785, 852, 993, 1112, 1205, 1313, 1357, 1415 |
| NciI | CCSGG | 2 cut(s) | 654, 1427 |
| NcoI | CCATGG | 1 cut(s) | 266 |
| NdeII | GATC | 8 cut(s) | 29, 159, 250, 292, 402, 526, 765, 1334 |
| NlaIII | CATG | 9 cut(s) | 270, 500, 509, 539, 926, 1103, 1215, 1240, 1299 |
| NmuCI | GTSAC | 1 cut(s) | 904 |
| NotI | GCGGCCGC | 1 cut(s) | 780 |
| NruI | TCGCGA | 2 cut(s) | 852, 993 |
| NsbI | TGCGCA | 1 cut(s) | 792 |
| NspI | RCATGY | 2 cut(s) | 1240, 1299 |
| PaeR7I | CTCGAG | 1 cut(s) | 917 |
| PagI | TCATGA | 1 cut(s) | 496 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 700 |
| PctI | GAATGC | 2 cut(s) | 460, 1145 |
| PfeI | GAWTC | 3 cut(s) | 182, 751, 1138 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 98, 131, 487 |
| PfoI | TCCNGGA | 1 cut(s) | 411 |
| PinAI | ACCGGT | 1 cut(s) | 22 |
| PleI | GAGTC | 1 cut(s) | 642 |
| PpsI | GAGTC | 1 cut(s) | 642 |
| Ppu21I | YACGTR | 1 cut(s) | 571 |
| Psp124BI | GAGCTC | 1 cut(s) | 673 |
| Psp6I | CCWGG | 4 cut(s) | 45, 411, 608, 1457 |
| PspEI | GGTNACC | 1 cut(s) | 1079 |
| PspGI | CCWGG | 4 cut(s) | 45, 411, 608, 1457 |
| PspPI | GGNCC | 7 cut(s) | 43, 478, 583, 823, 1000, 1227, 1430 |
| PstI | CTGCAG | 1 cut(s) | 1027 |
| PstNI | CAGNNNCTG | 1 cut(s) | 131 |
| PsuI | RGATCY | 4 cut(s) | 250, 402, 526, 1334 |
| RruI | TCGCGA | 2 cut(s) | 852, 993 |
| RsaI | GTAC | 3 cut(s) | 573, 680, 1186 |
| RsaNI | GTAC | 3 cut(s) | 572, 679, 1185 |
| SacI | GAGCTC | 1 cut(s) | 673 |
| SacII | CCGCGG | 1 cut(s) | 1416 |
| SaqAI | TTAA | 2 cut(s) | 683, 896 |
| Sau3AI | GATC | 8 cut(s) | 29, 159, 250, 292, 402, 526, 765, 1334 |
| Sau96I | GGNCC | 7 cut(s) | 43, 478, 583, 823, 1000, 1227, 1430 |
| ScaI | AGTACT | 2 cut(s) | 680, 1186 |
| SchI | GAGTC | 1 cut(s) | 643 |
| ScrFI | CCNGG | 6 cut(s) | 47, 413, 610, 654, 1427, 1459 |
| SduI | GDGCHC | 2 cut(s) | 621, 673 |
| SfaNI | GCATC | 2 cut(s) | 668, 1018 |
| SfcI | CTRYAG | 2 cut(s) | 746, 1023 |
| Sfr274I | CTCGAG | 1 cut(s) | 917 |
| Sfr303I | CCGCGG | 1 cut(s) | 1416 |
| SgrBI | CCGCGG | 1 cut(s) | 1416 |
| SinI | GGWCC | 2 cut(s) | 1227, 1430 |
| SlaI | CTCGAG | 1 cut(s) | 917 |
| SmlI | CTYRAG | 2 cut(s) | 807, 917 |
| SmoI | CTYRAG | 2 cut(s) | 807, 917 |
| Sse9I | AATT | 8 cut(s) | 60, 323, 407, 893, 1063, 1070, 1119, 1150 |
| SspMI | CTAG | 6 cut(s) | 563, 798, 938, 980, 1032, 1142 |
| SstI | GAGCTC | 1 cut(s) | 673 |
| StyD4I | CCNGG | 6 cut(s) | 45, 411, 608, 652, 1425, 1457 |
| StyI | CCWWGG | 2 cut(s) | 225, 266 |
| TaaI | ACNGT | 2 cut(s) | 157, 214 |
| TaiI | ACGT | 3 cut(s) | 40, 573, 628 |
| TaqI | TCGA | 5 cut(s) | 162, 256, 295, 879, 918 |
| TaqII | GACCGA | 1 cut(s) | 1302 |
| TasI | AATT | 8 cut(s) | 60, 323, 407, 893, 1063, 1070, 1119, 1150 |
| TatI | WGTACW | 2 cut(s) | 678, 1184 |
| TauI | GCSGC | 7 cut(s) | 479, 512, 782, 785, 1001, 1208, 1316 |
| TfiI | GAWTC | 3 cut(s) | 182, 751, 1138 |
| Tru1I | TTAA | 2 cut(s) | 683, 896 |
| Tru9I | TTAA | 2 cut(s) | 683, 896 |
| TscAI | CASTG | 3 cut(s) | 226, 537, 718 |
| TseFI | GTSAC | 1 cut(s) | 904 |
| TseI | GCWGC | 5 cut(s) | 512, 515, 518, 1019, 1022 |
| Tsp45I | GTSAC | 1 cut(s) | 904 |
| TspDTI | ATGAA | 5 cut(s) | 294, 513, 786, 1117, 1278 |
| TspGWI | ACGGA | 2 cut(s) | 1025, 1327 |
| TspRI | CASTG | 3 cut(s) | 226, 537, 718 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 98, 131, 487 |
| VpaK11BI | GGWCC | 2 cut(s) | 1227, 1430 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 983 |
| XceI | RCATGY | 2 cut(s) | 1240, 1299 |
| XhoI | CTCGAG | 1 cut(s) | 917 |
| XmiI | GTMKAC | 1 cut(s) | 1419 |
| XspI | CTAG | 6 cut(s) | 563, 798, 938, 980, 1032, 1142 |
| ZrmI | AGTACT | 2 cut(s) | 680, 1186 |