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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 331 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 3078, 3708 |
| Acc36I | ACCTGC | 5 cut(s) | 331, 1623, 3075, 3220, 3373 |
| AccB1I | GGYRCC | 5 cut(s) | 199, 243, 645, 920, 1111 |
| AccB7I | CCANNNNNTGG | 4 cut(s) | 1677, 2230, 2930, 2963 |
| AccBSI | CCGCTC | 1 cut(s) | 1694 |
| AccI | GTMKAC | 1 cut(s) | 954 |
| AccIII | TCCGGA | 1 cut(s) | 2972 |
| AclWI | GGATC | 5 cut(s) | 67, 2156, 2158, 2171, 3605 |
| AcoI | YGGCCR | 3 cut(s) | 1423, 1750, 2965 |
| AcsI | RAATTY | 4 cut(s) | 1310, 1973, 3378, 3806 |
| AcuI | CTGAAG | 3 cut(s) | 315, 1101, 2829 |
| AcyI | GRCGYC | 1 cut(s) | 200 |
| AdeI | CACNNNGTG | 1 cut(s) | 1677 |
| AfeI | AGCGCT | 2 cut(s) | 1879, 2383 |
| AflIII | ACRYGT | 1 cut(s) | 3310 |
| AgsI | TTSAA | 8 cut(s) | 1076, 1093, 1195, 2363, 2840, 3587, 3633, 3662 |
| AhlI | ACTAGT | 1 cut(s) | 2620 |
| AjnI | CCWGG | 8 cut(s) | 9, 706, 1620, 2546, 2877, 3315, 3692, 3736 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2160, 2192 |
| Alw26I | GTCTC | 6 cut(s) | 771, 797, 837, 1495, 2117, 2660 |
| AlwI | GGATC | 5 cut(s) | 67, 2156, 2158, 2171, 3605 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 713, 2963, 3122 |
| Aor13HI | TCCGGA | 1 cut(s) | 2972 |
| Aor51HI | AGCGCT | 2 cut(s) | 1879, 2383 |
| ApaI | GGGCCC | 1 cut(s) | 2583 |
| ApoI | RAATTY | 4 cut(s) | 1310, 1973, 3378, 3806 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2723, 2755 |
| AscI | GGCGCGCC | 1 cut(s) | 5 |
| AsiSI | GCGATCGC | 1 cut(s) | 2401 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 3806 |
| AsuC2I | CCSGG | 4 cut(s) | 1572, 1629, 2590, 3675 |
| AsuNHI | GCTAGC | 1 cut(s) | 3646 |
| AvaII | GGWCC | 5 cut(s) | 25, 874, 3087, 3148, 3419 |
| AxyI | CCTNAGG | 1 cut(s) | 3115 |
| BaeGI | GKGCMC | 3 cut(s) | 650, 1552, 2583 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1130, 1163 |
| BalI | TGGCCA | 2 cut(s) | 1752, 2967 |
| BamHI | GGATCC | 1 cut(s) | 2163 |
| BanI | GGYRCC | 5 cut(s) | 199, 243, 645, 920, 1111 |
| BanII | GRGCYC | 5 cut(s) | 2083, 2583, 2866, 2904, 3757 |
| BbsI | GAAGAC | 2 cut(s) | 1659, 2990 |
| BbvCI | CCTCAGC | 1 cut(s) | 54 |
| BceAI | ACGGC | 3 cut(s) | 1410, 2046, 2642 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 2777, 2811, 2835, 2869, 3187, 3221 |
| BciT130I | CCWGG | 8 cut(s) | 11, 708, 1622, 2548, 2879, 3317, 3694, 3738 |
| BciVI | GTATCC | 2 cut(s) | 899, 2672 |
| BcnI | CCSGG | 4 cut(s) | 1572, 1629, 2590, 3675 |
| BcoDI | GTCTC | 6 cut(s) | 771, 797, 837, 1495, 2117, 2660 |
| BcuI | ACTAGT | 1 cut(s) | 2620 |
| BfmI | CTRYAG | 3 cut(s) | 882, 1065, 2392 |
| BfoI | RGCGCY | 6 cut(s) | 203, 1881, 2385, 3123, 3306, 3332 |
| BfuAI | ACCTGC | 5 cut(s) | 331, 1623, 3075, 3220, 3373 |
| BfuI | GTATCC | 2 cut(s) | 899, 2672 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2508 |
| BglII | AGATCT | 2 cut(s) | 127, 1610 |
| BlpI | GCTNAGC | 1 cut(s) | 2243 |
| BmcAI | AGTACT | 5 cut(s) | 1396, 1961, 2334, 3453, 3764 |
| Bme18I | GGWCC | 5 cut(s) | 25, 874, 3087, 3148, 3419 |
| BmrI | ACTGGG | 4 cut(s) | 2345, 3111, 3218, 3560 |
| BmtI | GCTAGC | 1 cut(s) | 3650 |
| BmuI | ACTGGG | 4 cut(s) | 2345, 3111, 3218, 3560 |
| BoxI | GACNNNNGTC | 1 cut(s) | 789 |
| BpiI | GAAGAC | 2 cut(s) | 1659, 2990 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 3256, 3288 |
| BpmI | CTGGAG | 5 cut(s) | 339, 644, 2737, 2940, 3378 |
| Bpu10I | CCTNAGC | 3 cut(s) | 54, 651, 2643 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2243 |
| BpuEI | CTTGAG | 6 cut(s) | 1019, 1207, 1481, 2892, 3342, 3787 |
| BpuMI | CCSGG | 4 cut(s) | 1572, 1629, 2590, 3675 |
| Bsa29I | ATCGAT | 3 cut(s) | 1651, 2652, 3063 |
| BsaHI | GRCGYC | 1 cut(s) | 200 |
| BsaI | GGTCTC | 2 cut(s) | 771, 837 |
| BsaWI | WCCGGW | 1 cut(s) | 2972 |
| Bse118I | RCCGGY | 3 cut(s) | 2215, 2842, 3479 |
| Bse21I | CCTNAGG | 1 cut(s) | 3115 |
| Bse3DI | GCAATG | 2 cut(s) | 610, 662 |
| BseAI | TCCGGA | 1 cut(s) | 2972 |
| BseBI | CCWGG | 8 cut(s) | 11, 708, 1622, 2548, 2879, 3317, 3694, 3738 |
| BseCI | ATCGAT | 3 cut(s) | 1651, 2652, 3063 |
| BseMI | GCAATG | 2 cut(s) | 610, 662 |
| BseMII | CTCAG | 7 cut(s) | 45, 642, 1239, 2257, 2661, 3129, 3789 |
| BsePI | GCGCGC | 3 cut(s) | 5, 2403, 2405 |
| BseSI | GKGCMC | 3 cut(s) | 650, 1552, 2583 |
| BseYI | CCCAGC | 3 cut(s) | 139, 2350, 3475 |
| BsgI | GTGCAG | 4 cut(s) | 2407, 2802, 2961, 3248 |
| Bsh1285I | CGRYCG | 3 cut(s) | 958, 1523, 2401 |
| BshNI | GGYRCC | 5 cut(s) | 199, 243, 645, 920, 1111 |
| BshVI | ATCGAT | 3 cut(s) | 1651, 2652, 3063 |
| BsiEI | CGRYCG | 3 cut(s) | 958, 1523, 2401 |
| BsiSI | CCGG | 9 cut(s) | 1572, 1629, 1921, 2216, 2589, 2843, 2973, 3480, 3675 |
| BslFI | GGGAC | 5 cut(s) | 669, 875, 2741, 3705, 3710 |
| BsmAI | GTCTC | 6 cut(s) | 771, 797, 837, 1495, 2117, 2660 |
| BsmFI | GGGAC | 5 cut(s) | 669, 875, 2741, 3705, 3710 |
| BsmI | GAATGC | 2 cut(s) | 2288, 3273 |
| Bso31I | GGTCTC | 2 cut(s) | 771, 837 |
| Bsp120I | GGGCCC | 1 cut(s) | 2579 |
| Bsp1286I | GDGCHC | 7 cut(s) | 650, 1552, 2083, 2583, 2866, 2904, 3757 |
| Bsp13I | TCCGGA | 1 cut(s) | 2972 |
| Bsp1407I | TGTACA | 1 cut(s) | 2602 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2243 |
| Bsp19I | CCATGG | 2 cut(s) | 766, 1857 |
| Bsp68I | TCGCGA | 5 cut(s) | 1594, 2744, 2898, 3197, 3783 |
| BspCNI | CTCAG | 7 cut(s) | 46, 643, 1240, 2256, 2662, 3128, 3790 |
| BspDI | ATCGAT | 3 cut(s) | 1651, 2652, 3063 |
| BspEI | TCCGGA | 1 cut(s) | 2972 |
| BspHI | TCATGA | 1 cut(s) | 3289 |
| BspMI | ACCTGC | 5 cut(s) | 331, 1623, 3075, 3220, 3373 |
| BspOI | GCTAGC | 1 cut(s) | 3650 |
| BspPI | GGATC | 5 cut(s) | 67, 2156, 2158, 2171, 3605 |
| BspQI | GCTCTTC | 1 cut(s) | 917 |
| BspT107I | GGYRCC | 5 cut(s) | 199, 243, 645, 920, 1111 |
| BspTNI | GGTCTC | 2 cut(s) | 771, 837 |
| BsrBI | CCGCTC | 1 cut(s) | 1694 |
| BsrDI | GCAATG | 2 cut(s) | 610, 662 |
| BsrFI | RCCGGY | 3 cut(s) | 2215, 2842, 3479 |
| BsrGI | TGTACA | 1 cut(s) | 2602 |
| BssAI | RCCGGY | 3 cut(s) | 2215, 2842, 3479 |
| BssHII | GCGCGC | 3 cut(s) | 5, 2403, 2405 |
| BssNI | GRCGYC | 1 cut(s) | 200 |
| BssT1I | CCWWGG | 3 cut(s) | 766, 1857, 3000 |
| Bst2UI | CCWGG | 8 cut(s) | 11, 708, 1622, 2548, 2879, 3317, 3694, 3738 |
| Bst4CI | ACNGT | 4 cut(s) | 952, 1521, 1886, 1902 |
| Bst6I | CTCTTC | 5 cut(s) | 851, 917, 2025, 2744, 3255 |
| BstACI | GRCGYC | 1 cut(s) | 200 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2948 |
| BstAUI | TGTACA | 1 cut(s) | 2602 |
| BstDSI | CCRYGG | 7 cut(s) | 567, 766, 1426, 1857, 1898, 2658, 3159 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 2073, 3525 |
| BstH2I | RGCGCY | 6 cut(s) | 203, 1881, 2385, 3123, 3306, 3332 |
| BstMAI | GTCTC | 6 cut(s) | 771, 797, 837, 1495, 2117, 2660 |
| BstMCI | CGRYCG | 3 cut(s) | 958, 1523, 2401 |
| BstNI | CCWGG | 8 cut(s) | 11, 708, 1622, 2548, 2879, 3317, 3694, 3738 |
| BstNSI | RCATGY | 4 cut(s) | 150, 681, 2614, 3314 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 789 |
| BstSFI | CTRYAG | 3 cut(s) | 882, 1065, 2392 |
| BstSLI | GKGCMC | 3 cut(s) | 650, 1552, 2583 |
| BstV2I | GAAGAC | 2 cut(s) | 1659, 2990 |
| BstX2I | RGATCY | 6 cut(s) | 59, 127, 1610, 2148, 2163, 3610 |
| BstYI | RGATCY | 6 cut(s) | 59, 127, 1610, 2148, 2163, 3610 |
| Bsu15I | ATCGAT | 3 cut(s) | 1651, 2652, 3063 |
| Bsu36I | CCTNAGG | 1 cut(s) | 3115 |
| BsuI | GTATCC | 2 cut(s) | 899, 2672 |
| BsuTUI | ATCGAT | 3 cut(s) | 1651, 2652, 3063 |
| BtgI | CCRYGG | 7 cut(s) | 567, 766, 1426, 1857, 1898, 2658, 3159 |
| BtgZI | GCGATG | 1 cut(s) | 3764 |
| BtsI | GCAGTG | 2 cut(s) | 1110, 2056 |
| BtuMI | TCGCGA | 5 cut(s) | 1594, 2744, 2898, 3197, 3783 |
| BveI | ACCTGC | 5 cut(s) | 331, 1623, 3075, 3220, 3373 |
| CaiI | CAGNNNCTG | 3 cut(s) | 713, 2963, 3122 |
| CciI | TCATGA | 1 cut(s) | 3289 |
| Cfr10I | RCCGGY | 3 cut(s) | 2215, 2842, 3479 |
| Cfr42I | CCGCGG | 1 cut(s) | 570 |
| ClaI | ATCGAT | 3 cut(s) | 1651, 2652, 3063 |
| CseI | GACGC | 3 cut(s) | 661, 3181, 3533 |
| CsiI | ACCWGGT | 1 cut(s) | 706 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2480, 2515 |
| DinI | GGCGCC | 1 cut(s) | 201 |
| DraIII | CACNNNGTG | 1 cut(s) | 1677 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 3078, 3708 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 3078, 3708 |
| EaeI | YGGCCR | 3 cut(s) | 1423, 1750, 2965 |
| Eam1104I | CTCTTC | 5 cut(s) | 851, 917, 2025, 2744, 3255 |
| EarI | CTCTTC | 5 cut(s) | 851, 917, 2025, 2744, 3255 |
| Eco130I | CCWWGG | 3 cut(s) | 766, 1857, 3000 |
| Eco24I | GRGCYC | 5 cut(s) | 2083, 2583, 2866, 2904, 3757 |
| Eco31I | GGTCTC | 2 cut(s) | 771, 837 |
| Eco47I | GGWCC | 5 cut(s) | 25, 874, 3087, 3148, 3419 |
| Eco47III | AGCGCT | 2 cut(s) | 1879, 2383 |
| Eco57I | CTGAAG | 3 cut(s) | 315, 1101, 2829 |
| Eco81I | CCTNAGG | 1 cut(s) | 3115 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 2073, 3525 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2580 |
| EcoRI | GAATTC | 1 cut(s) | 3806 |
| EcoRII | CCWGG | 8 cut(s) | 9, 706, 1620, 2546, 2877, 3315, 3692, 3736 |
| EcoT14I | CCWWGG | 3 cut(s) | 766, 1857, 3000 |
| EcoT22I | ATGCAT | 1 cut(s) | 2115 |
| EcoT38I | GRGCYC | 5 cut(s) | 2083, 2583, 2866, 2904, 3757 |
| EgeI | GGCGCC | 1 cut(s) | 201 |
| EheI | GGCGCC | 1 cut(s) | 201 |
| ErhI | CCWWGG | 3 cut(s) | 766, 1857, 3000 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 848, 880 |
| FaqI | GGGAC | 5 cut(s) | 669, 875, 2741, 3705, 3710 |
| FauI | CCCGC | 5 cut(s) | 562, 2042, 2211, 2874, 3014 |
| FblI | GTMKAC | 1 cut(s) | 954 |
| FriOI | GRGCYC | 5 cut(s) | 2083, 2583, 2866, 2904, 3757 |
| GsaI | CCCAGC | 3 cut(s) | 143, 2354, 3479 |
| GsuI | CTGGAG | 5 cut(s) | 339, 644, 2737, 2940, 3378 |
| HaeII | RGCGCY | 6 cut(s) | 203, 1881, 2385, 3123, 3306, 3332 |
| HapII | CCGG | 9 cut(s) | 1572, 1629, 1921, 2216, 2589, 2843, 2973, 3480, 3675 |
| HgaI | GACGC | 3 cut(s) | 661, 3181, 3533 |
| Hin1I | GRCGYC | 1 cut(s) | 200 |
| HincII | GTYRAC | 4 cut(s) | 667, 955, 2723, 3496 |
| HindII | GTYRAC | 4 cut(s) | 667, 955, 2723, 3496 |
| HpaI | GTTAAC | 1 cut(s) | 3496 |
| HpaII | CCGG | 9 cut(s) | 1572, 1629, 1921, 2216, 2589, 2843, 2973, 3480, 3675 |
| Hpy166II | GTNNAC | 7 cut(s) | 474, 667, 955, 1689, 2723, 3090, 3496 |
| Hpy188I | TCNGA | 9 cut(s) | 562, 802, 836, 1339, 2281, 2440, 2809, 3025, 3280 |
| Hpy8I | GTNNAC | 7 cut(s) | 474, 667, 955, 1689, 2723, 3090, 3496 |
| Hpy99I | CGWCG | 7 cut(s) | 400, 956, 983, 2032, 2735, 3084, 3197 |
| HpyCH4III | ACNGT | 4 cut(s) | 952, 1521, 1886, 1902 |
| HpyCH4IV | ACGT | 2 cut(s) | 1257, 3709 |
| HpySE526I | ACGT | 2 cut(s) | 1257, 3709 |
| Hsp92I | GRCGYC | 1 cut(s) | 200 |
| KasI | GGCGCC | 1 cut(s) | 199 |
| Kpn2I | TCCGGA | 1 cut(s) | 2972 |
| KspAI | GTTAAC | 1 cut(s) | 3496 |
| KspI | CCGCGG | 1 cut(s) | 570 |
| LguI | GCTCTTC | 1 cut(s) | 917 |
| MabI | ACCWGGT | 1 cut(s) | 706 |
| MaeII | ACGT | 2 cut(s) | 1257, 3709 |
| MaeIII | GTNAC | 7 cut(s) | 116, 375, 399, 673, 692, 1442, 3664 |
| MauBI | CGCGCGCG | 1 cut(s) | 2403 |
| MbiI | CCGCTC | 1 cut(s) | 1694 |
| MfeI | CAATTG | 2 cut(s) | 2710, 3209 |
| MflI | RGATCY | 6 cut(s) | 59, 127, 1610, 2148, 2163, 3610 |
| MhlI | GDGCHC | 7 cut(s) | 650, 1552, 2083, 2583, 2866, 2904, 3757 |
| MlsI | TGGCCA | 2 cut(s) | 1752, 2967 |
| MluNI | TGGCCA | 2 cut(s) | 1752, 2967 |
| Mly113I | GGCGCC | 1 cut(s) | 200 |
| MlyI | GAGTC | 9 cut(s) | 591, 1026, 1139, 1376, 2011, 2728, 3661, 3696, 3727 |
| MmeI | TCCRAC | 2 cut(s) | 831, 3730 |
| Mox20I | TGGCCA | 2 cut(s) | 1752, 2967 |
| Mph1103I | ATGCAT | 1 cut(s) | 2115 |
| MroI | TCCGGA | 1 cut(s) | 2972 |
| MroXI | GAANNNNTTC | 1 cut(s) | 3806 |
| MscI | TGGCCA | 2 cut(s) | 1752, 2967 |
| MseI | TTAA | 2 cut(s) | 1221, 3495 |
| MslI | CAYNNNNRTG | 3 cut(s) | 615, 1146, 3279 |
| Msp20I | TGGCCA | 2 cut(s) | 1752, 2967 |
| MspA1I | CMGCKG | 2 cut(s) | 569, 2206 |
| MspI | CCGG | 9 cut(s) | 1572, 1629, 1921, 2216, 2589, 2843, 2973, 3480, 3675 |
| MunI | CAATTG | 2 cut(s) | 2710, 3209 |
| Mva1269I | GAATGC | 2 cut(s) | 2288, 3273 |
| MvaI | CCWGG | 8 cut(s) | 11, 708, 1622, 2548, 2879, 3317, 3694, 3738 |
| NarI | GGCGCC | 1 cut(s) | 200 |
| NciI | CCSGG | 4 cut(s) | 1572, 1629, 2590, 3675 |
| NcoI | CCATGG | 2 cut(s) | 766, 1857 |
| NheI | GCTAGC | 1 cut(s) | 3646 |
| NmuCI | GTSAC | 4 cut(s) | 375, 399, 673, 3664 |
| NruI | TCGCGA | 5 cut(s) | 1594, 2744, 2898, 3197, 3783 |
| NsiI | ATGCAT | 1 cut(s) | 2115 |
| NspI | RCATGY | 4 cut(s) | 150, 681, 2614, 3314 |
| PaeI | GCATGC | 2 cut(s) | 150, 2614 |
| PagI | TCATGA | 1 cut(s) | 3289 |
| PalAI | GGCGCGCC | 1 cut(s) | 5 |
| PaqCI | CACCTGC | 1 cut(s) | 331 |
| PauI | GCGCGC | 3 cut(s) | 5, 2403, 2405 |
| PciI | ACATGT | 1 cut(s) | 3310 |
| PciSI | GCTCTTC | 1 cut(s) | 917 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 404, 1598, 3076 |
| PctI | GAATGC | 2 cut(s) | 2288, 3273 |
| PdmI | GAANNNNTTC | 1 cut(s) | 3806 |
| PfeI | GAWTC | 6 cut(s) | 803, 2346, 2370, 2574, 2893, 3412 |
| PflFI | GACNNNGTC | 2 cut(s) | 671, 950 |
| PflMI | CCANNNNNTGG | 4 cut(s) | 1677, 2230, 2930, 2963 |
| PfoI | TCCNGGA | 1 cut(s) | 3673 |
| Ple19I | CGATCG | 1 cut(s) | 2401 |
| PleI | GAGTC | 9 cut(s) | 590, 1026, 1138, 1375, 2011, 2727, 3661, 3696, 3726 |
| PluTI | GGCGCC | 1 cut(s) | 203 |
| PpsI | GAGTC | 9 cut(s) | 590, 1026, 1138, 1375, 2011, 2727, 3661, 3696, 3726 |
| PscI | ACATGT | 1 cut(s) | 3310 |
| PshAI | GACNNNNGTC | 1 cut(s) | 789 |
| Psp6I | CCWGG | 8 cut(s) | 9, 706, 1620, 2546, 2877, 3315, 3692, 3736 |
| PspFI | CCCAGC | 3 cut(s) | 139, 2350, 3475 |
| PspGI | CCWGG | 8 cut(s) | 9, 706, 1620, 2546, 2877, 3315, 3692, 3736 |
| PspOMI | GGGCCC | 1 cut(s) | 2579 |
| PstNI | CAGNNNCTG | 3 cut(s) | 713, 2963, 3122 |
| PsuI | RGATCY | 6 cut(s) | 59, 127, 1610, 2148, 2163, 3610 |
| PsyI | GACNNNGTC | 2 cut(s) | 671, 950 |
| PteI | GCGCGC | 3 cut(s) | 5, 2403, 2405 |
| PvuI | CGATCG | 1 cut(s) | 2401 |
| RgaI | GCGATCGC | 1 cut(s) | 2401 |
| RruI | TCGCGA | 5 cut(s) | 1594, 2744, 2898, 3197, 3783 |
| RseI | CAYNNNNRTG | 3 cut(s) | 615, 1146, 3279 |
| SacII | CCGCGG | 1 cut(s) | 570 |
| SalI | GTCGAC | 1 cut(s) | 953 |
| SapI | GCTCTTC | 1 cut(s) | 917 |
| SaqAI | TTAA | 2 cut(s) | 1221, 3495 |
| ScaI | AGTACT | 5 cut(s) | 1396, 1961, 2334, 3453, 3764 |
| SchI | GAGTC | 9 cut(s) | 591, 1026, 1139, 1376, 2011, 2728, 3661, 3696, 3727 |
| SduI | GDGCHC | 7 cut(s) | 650, 1552, 2083, 2583, 2866, 2904, 3757 |
| SexAI | ACCWGGT | 1 cut(s) | 706 |
| SfaAI | GCGATCGC | 1 cut(s) | 2401 |
| SfcI | CTRYAG | 3 cut(s) | 882, 1065, 2392 |
| SfoI | GGCGCC | 1 cut(s) | 201 |
| Sfr303I | CCGCGG | 1 cut(s) | 570 |
| SgfI | GCGATCGC | 1 cut(s) | 2401 |
| SgrBI | CCGCGG | 1 cut(s) | 570 |
| SgsI | GGCGCGCC | 1 cut(s) | 5 |
| SinI | GGWCC | 5 cut(s) | 25, 874, 3087, 3148, 3419 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 615, 1146, 3279 |
| SmlI | CTYRAG | 6 cut(s) | 1034, 1186, 1496, 2907, 3357, 3766 |
| SmoI | CTYRAG | 6 cut(s) | 1034, 1186, 1496, 2907, 3357, 3766 |
| SpeI | ACTAGT | 1 cut(s) | 2620 |
| SphI | GCATGC | 2 cut(s) | 150, 2614 |
| SspDI | GGCGCC | 1 cut(s) | 199 |
| StyI | CCWWGG | 3 cut(s) | 766, 1857, 3000 |
| TaaI | ACNGT | 4 cut(s) | 952, 1521, 1886, 1902 |
| TaiI | ACGT | 2 cut(s) | 1260, 3712 |
| TatI | WGTACW | 7 cut(s) | 1394, 1959, 1985, 2332, 2602, 3451, 3762 |
| TfiI | GAWTC | 6 cut(s) | 803, 2346, 2370, 2574, 2893, 3412 |
| Tru1I | TTAA | 2 cut(s) | 1221, 3495 |
| Tru9I | TTAA | 2 cut(s) | 1221, 3495 |
| TseFI | GTSAC | 4 cut(s) | 375, 399, 673, 3664 |
| Tsp45I | GTSAC | 4 cut(s) | 375, 399, 673, 3664 |
| TspGWI | ACGGA | 4 cut(s) | 363, 1136, 3714, 3736 |
| Tth111I | GACNNNGTC | 2 cut(s) | 671, 950 |
| Van91I | CCANNNNNTGG | 4 cut(s) | 1677, 2230, 2930, 2963 |
| VpaK11BI | GGWCC | 5 cut(s) | 25, 874, 3087, 3148, 3419 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 2073, 3525 |
| XapI | RAATTY | 4 cut(s) | 1310, 1973, 3378, 3806 |
| XbaI | TCTAGA | 1 cut(s) | 3757 |
| XceI | RCATGY | 4 cut(s) | 150, 681, 2614, 3314 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 936, 2879 |
| XmiI | GTMKAC | 1 cut(s) | 954 |
| XmnI | GAANNNNTTC | 1 cut(s) | 3806 |
| ZrmI | AGTACT | 5 cut(s) | 1396, 1961, 2334, 3453, 3764 |
| Zsp2I | ATGCAT | 1 cut(s) | 2115 |