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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 4 cut(s) | 292, 433, 449, 493 |
| AclI | AACGTT | 1 cut(s) | 375 |
| AclWI | GGATC | 2 cut(s) | 403, 416 |
| AcoI | YGGCCR | 2 cut(s) | 289, 462 |
| AcsI | RAATTY | 1 cut(s) | 218 |
| AfaI | GTAC | 2 cut(s) | 256, 318 |
| AfeI | AGCGCT | 1 cut(s) | 124 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 50, 258, 320 |
| AgeI | ACCGGT | 1 cut(s) | 251 |
| AgsI | TTSAA | 1 cut(s) | 425 |
| AlwI | GGATC | 2 cut(s) | 403, 416 |
| Ama87I | CYCGRG | 1 cut(s) | 142 |
| Aor51HI | AGCGCT | 1 cut(s) | 124 |
| AoxI | GGCC | 6 cut(s) | 37, 105, 276, 289, 324, 462 |
| ApaI | GGGCCC | 1 cut(s) | 328 |
| ApeKI | GCWGC | 1 cut(s) | 120 |
| ApoI | RAATTY | 1 cut(s) | 218 |
| AsiGI | ACCGGT | 1 cut(s) | 251 |
| AspLEI | GCGC | 1 cut(s) | 125 |
| AspS9I | GGNCC | 6 cut(s) | 106, 261, 277, 324, 325, 383 |
| AsuC2I | CCSGG | 1 cut(s) | 167 |
| AvaI | CYCGRG | 1 cut(s) | 142 |
| AvaII | GGWCC | 2 cut(s) | 261, 383 |
| BaeGI | GKGCMC | 1 cut(s) | 328 |
| BamHI | GGATCC | 1 cut(s) | 408 |
| BanII | GRGCYC | 3 cut(s) | 60, 306, 328 |
| BbvI | GCAGC | 1 cut(s) | 132 |
| BccI | CCATC | 4 cut(s) | 38, 53, 141, 477 |
| BceAI | ACGGC | 4 cut(s) | 67, 291, 304, 420 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 471 |
| BcnI | CCSGG | 1 cut(s) | 167 |
| BfaI | CTAG | 1 cut(s) | 496 |
| BfoI | RGCGCY | 1 cut(s) | 126 |
| BisI | GCNGC | 3 cut(s) | 121, 292, 493 |
| BlsI | GCNGC | 3 cut(s) | 122, 293, 494 |
| Bme1390I | CCNGG | 1 cut(s) | 167 |
| Bme18I | GGWCC | 2 cut(s) | 261, 383 |
| BmeT110I | CYCGRG | 1 cut(s) | 142 |
| BmgT120I | GGNCC | 6 cut(s) | 106, 261, 277, 324, 325, 383 |
| BmiI | GGNNCC | 4 cut(s) | 325, 326, 385, 410 |
| BmrFI | CCNGG | 1 cut(s) | 167 |
| BmrI | ACTGGG | 1 cut(s) | 329 |
| BmsI | GCATC | 2 cut(s) | 345, 367 |
| BmuI | ACTGGG | 1 cut(s) | 329 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 27 |
| BpuEI | CTTGAG | 1 cut(s) | 221 |
| BpuMI | CCSGG | 1 cut(s) | 167 |
| BsaJI | CCNNGG | 1 cut(s) | 102 |
| BsaWI | WCCGGW | 2 cut(s) | 251, 380 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 50, 258, 320 |
| Bse118I | RCCGGY | 2 cut(s) | 251, 460 |
| Bse1I | ACTGG | 2 cut(s) | 40, 324 |
| BseDI | CCNNGG | 1 cut(s) | 102 |
| BseGI | GGATG | 2 cut(s) | 21, 358 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 50, 258, 320 |
| BseNI | ACTGG | 2 cut(s) | 40, 324 |
| BseRI | GAGGAG | 1 cut(s) | 226 |
| BseSI | GKGCMC | 1 cut(s) | 328 |
| BseX3I | CGGCCG | 2 cut(s) | 289, 462 |
| BseXI | GCAGC | 1 cut(s) | 132 |
| BseYI | CCCAGC | 1 cut(s) | 108 |
| BsgI | GTGCAG | 1 cut(s) | 214 |
| Bsh1285I | CGRYCG | 3 cut(s) | 183, 292, 465 |
| BshFI | GGCC | 6 cut(s) | 39, 107, 278, 291, 326, 464 |
| BshTI | ACCGGT | 1 cut(s) | 251 |
| BsiEI | CGRYCG | 3 cut(s) | 183, 292, 465 |
| BsiHKCI | CYCGRG | 1 cut(s) | 142 |
| BsiSI | CCGG | 4 cut(s) | 166, 252, 381, 461 |
| BslFI | GGGAC | 1 cut(s) | 369 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 50, 258, 320 |
| BsmFI | GGGAC | 1 cut(s) | 369 |
| BsnI | GGCC | 6 cut(s) | 39, 107, 278, 291, 326, 464 |
| BsoBI | CYCGRG | 1 cut(s) | 142 |
| Bsp120I | GGGCCC | 1 cut(s) | 324 |
| Bsp1286I | GDGCHC | 3 cut(s) | 60, 306, 328 |
| Bsp143I | GATC | 3 cut(s) | 180, 369, 408 |
| Bsp19I | CCATGG | 1 cut(s) | 102 |
| BspACI | CCGC | 4 cut(s) | 292, 433, 449, 493 |
| BspANI | GGCC | 6 cut(s) | 39, 107, 278, 291, 326, 464 |
| BspLI | GGNNCC | 4 cut(s) | 325, 326, 385, 410 |
| BspPI | GGATC | 2 cut(s) | 403, 416 |
| BsrFI | RCCGGY | 2 cut(s) | 251, 460 |
| BsrI | ACTGG | 2 cut(s) | 40, 324 |
| BssAI | RCCGGY | 2 cut(s) | 251, 460 |
| BssECI | CCNNGG | 1 cut(s) | 102 |
| BssMI | GATC | 3 cut(s) | 180, 369, 408 |
| BssT1I | CCWWGG | 1 cut(s) | 102 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 457 |
| BstC8I | GCNNGC | 3 cut(s) | 56, 338, 462 |
| BstDSI | CCRYGG | 1 cut(s) | 102 |
| BstF5I | GGATG | 2 cut(s) | 21, 358 |
| BstH2I | RGCGCY | 1 cut(s) | 126 |
| BstHHI | GCGC | 1 cut(s) | 125 |
| BstKTI | GATC | 3 cut(s) | 183, 372, 411 |
| BstMBI | GATC | 3 cut(s) | 180, 369, 408 |
| BstMCI | CGRYCG | 3 cut(s) | 183, 292, 465 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 64, 457 |
| BstNSI | RCATGY | 1 cut(s) | 340 |
| BstSCI | CCNGG | 1 cut(s) | 165 |
| BstSLI | GKGCMC | 1 cut(s) | 328 |
| BstV1I | GCAGC | 1 cut(s) | 132 |
| BstX2I | RGATCY | 1 cut(s) | 408 |
| BstYI | RGATCY | 1 cut(s) | 408 |
| BstZI | CGGCCG | 2 cut(s) | 289, 462 |
| BsuRI | GGCC | 6 cut(s) | 39, 107, 278, 291, 326, 464 |
| BtgI | CCRYGG | 1 cut(s) | 102 |
| BtsCI | GGATG | 2 cut(s) | 21, 358 |
| BtsIMutI | CAGTG | 1 cut(s) | 33 |
| Cac8I | GCNNGC | 3 cut(s) | 56, 338, 462 |
| CfoI | GCGC | 1 cut(s) | 125 |
| Cfr10I | RCCGGY | 2 cut(s) | 251, 460 |
| Cfr13I | GGNCC | 6 cut(s) | 106, 261, 277, 324, 325, 383 |
| Csp6I | GTAC | 2 cut(s) | 255, 317 |
| CspAI | ACCGGT | 1 cut(s) | 251 |
| CviAII | CATG | 3 cut(s) | 68, 103, 337 |
| CviQI | GTAC | 2 cut(s) | 255, 317 |
| DpnI | GATC | 3 cut(s) | 182, 371, 410 |
| DpnII | GATC | 3 cut(s) | 180, 369, 408 |
| EaeI | YGGCCR | 2 cut(s) | 289, 462 |
| EagI | CGGCCG | 2 cut(s) | 289, 462 |
| EciI | GGCGGA | 1 cut(s) | 422 |
| EclXI | CGGCCG | 2 cut(s) | 289, 462 |
| Eco130I | CCWWGG | 1 cut(s) | 102 |
| Eco24I | GRGCYC | 3 cut(s) | 60, 306, 328 |
| Eco32I | GATATC | 1 cut(s) | 445 |
| Eco47I | GGWCC | 2 cut(s) | 261, 383 |
| Eco47III | AGCGCT | 1 cut(s) | 124 |
| Eco52I | CGGCCG | 2 cut(s) | 289, 462 |
| Eco88I | CYCGRG | 1 cut(s) | 142 |
| EcoO109I | RGGNCCY | 2 cut(s) | 324, 325 |
| EcoRV | GATATC | 1 cut(s) | 445 |
| EcoT14I | CCWWGG | 1 cut(s) | 102 |
| EcoT22I | ATGCAT | 1 cut(s) | 360 |
| EcoT38I | GRGCYC | 3 cut(s) | 60, 306, 328 |
| ErhI | CCWWGG | 1 cut(s) | 102 |
| FaeI | CATG | 3 cut(s) | 71, 106, 340 |
| FaqI | GGGAC | 1 cut(s) | 369 |
| FatI | CATG | 3 cut(s) | 67, 102, 336 |
| FauI | CCCGC | 1 cut(s) | 456 |
| Fnu4HI | GCNGC | 3 cut(s) | 121, 292, 493 |
| FokI | GGATG | 2 cut(s) | 8, 345 |
| FriOI | GRGCYC | 3 cut(s) | 60, 306, 328 |
| Fsp4HI | GCNGC | 3 cut(s) | 121, 292, 493 |
| FspBI | CTAG | 1 cut(s) | 496 |
| GlaI | GCGC | 1 cut(s) | 124 |
| GluI | GCNGC | 3 cut(s) | 121, 292, 493 |
| GsaI | CCCAGC | 1 cut(s) | 112 |
| HaeII | RGCGCY | 1 cut(s) | 126 |
| HaeIII | GGCC | 6 cut(s) | 39, 107, 278, 291, 326, 464 |
| HapII | CCGG | 4 cut(s) | 166, 252, 381, 461 |
| HhaI | GCGC | 1 cut(s) | 125 |
| Hin1II | CATG | 3 cut(s) | 71, 106, 340 |
| Hin6I | GCGC | 1 cut(s) | 123 |
| HinP1I | GCGC | 1 cut(s) | 123 |
| HinfI | GANTC | 2 cut(s) | 80, 189 |
| HpaII | CCGG | 4 cut(s) | 166, 252, 381, 461 |
| Hpy188I | TCNGA | 2 cut(s) | 185, 308 |
| Hpy188III | TCNNGA | 4 cut(s) | 367, 391, 406, 412 |
| Hpy99I | CGWCG | 1 cut(s) | 53 |
| HpyAV | CCTTC | 2 cut(s) | 373, 397 |
| HpyCH4IV | ACGT | 1 cut(s) | 375 |
| HpyCH4V | TGCA | 4 cut(s) | 67, 216, 231, 358 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 64, 457 |
| HpySE526I | ACGT | 1 cut(s) | 375 |
| Hsp92II | CATG | 3 cut(s) | 71, 106, 340 |
| HspAI | GCGC | 1 cut(s) | 123 |
| KroI | GCCGGC | 1 cut(s) | 460 |
| KroNI | GCCGGC | 1 cut(s) | 462 |
| Kzo9I | GATC | 3 cut(s) | 180, 369, 408 |
| Lsp1109I | GCAGC | 1 cut(s) | 132 |
| LweI | GCATC | 2 cut(s) | 345, 367 |
| MaeI | CTAG | 1 cut(s) | 496 |
| MaeII | ACGT | 1 cut(s) | 375 |
| MaeIII | GTNAC | 1 cut(s) | 7 |
| MalI | GATC | 3 cut(s) | 182, 371, 410 |
| MboI | GATC | 3 cut(s) | 180, 369, 408 |
| MboII | GAAGA | 2 cut(s) | 421, 452 |
| MfeI | CAATTG | 1 cut(s) | 470 |
| MflI | RGATCY | 1 cut(s) | 408 |
| MhlI | GDGCHC | 3 cut(s) | 60, 306, 328 |
| MluCI | AATT | 4 cut(s) | 74, 218, 425, 470 |
| MlyI | GAGTC | 1 cut(s) | 74 |
| MnlI | CCTC | 5 cut(s) | 35, 179, 244, 247, 290 |
| Mph1103I | ATGCAT | 1 cut(s) | 360 |
| MroNI | GCCGGC | 1 cut(s) | 460 |
| MspI | CCGG | 4 cut(s) | 166, 252, 381, 461 |
| MspR9I | CCNGG | 1 cut(s) | 167 |
| MunI | CAATTG | 1 cut(s) | 470 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 64, 457 |
| NaeI | GCCGGC | 1 cut(s) | 462 |
| NciI | CCSGG | 1 cut(s) | 167 |
| NcoI | CCATGG | 1 cut(s) | 102 |
| NdeII | GATC | 3 cut(s) | 180, 369, 408 |
| NgoMIV | GCCGGC | 1 cut(s) | 460 |
| NlaIII | CATG | 3 cut(s) | 71, 106, 340 |
| NlaIV | GGNNCC | 4 cut(s) | 325, 326, 385, 410 |
| NsiI | ATGCAT | 1 cut(s) | 360 |
| NspI | RCATGY | 1 cut(s) | 340 |
| PaeI | GCATGC | 1 cut(s) | 340 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 372 |
| PdiI | GCCGGC | 1 cut(s) | 462 |
| PfeI | GAWTC | 1 cut(s) | 189 |
| PflFI | GACNNNGTC | 1 cut(s) | 83 |
| PinAI | ACCGGT | 1 cut(s) | 251 |
| PkrI | GCNGC | 3 cut(s) | 122, 293, 494 |
| Ple19I | CGATCG | 1 cut(s) | 183 |
| PleI | GAGTC | 1 cut(s) | 74 |
| PpsI | GAGTC | 1 cut(s) | 74 |
| Psp1406I | AACGTT | 1 cut(s) | 375 |
| PspFI | CCCAGC | 1 cut(s) | 108 |
| PspN4I | GGNNCC | 4 cut(s) | 325, 326, 385, 410 |
| PspOMI | GGGCCC | 1 cut(s) | 324 |
| PspPI | GGNCC | 6 cut(s) | 106, 261, 277, 324, 325, 383 |
| PsuI | RGATCY | 1 cut(s) | 408 |
| PsyI | GACNNNGTC | 1 cut(s) | 83 |
| PvuI | CGATCG | 1 cut(s) | 183 |
| RsaI | GTAC | 2 cut(s) | 256, 318 |
| RsaNI | GTAC | 2 cut(s) | 255, 317 |
| SatI | GCNGC | 3 cut(s) | 121, 292, 493 |
| Sau3AI | GATC | 3 cut(s) | 180, 369, 408 |
| Sau96I | GGNCC | 6 cut(s) | 106, 261, 277, 324, 325, 383 |
| SchI | GAGTC | 1 cut(s) | 74 |
| ScrFI | CCNGG | 1 cut(s) | 167 |
| SduI | GDGCHC | 3 cut(s) | 60, 306, 328 |
| SetI | ASST | 6 cut(s) | 118, 236, 266, 316, 378, 481 |
| SfaNI | GCATC | 2 cut(s) | 345, 367 |
| SinI | GGWCC | 2 cut(s) | 261, 383 |
| SmlI | CTYRAG | 1 cut(s) | 200 |
| SmoI | CTYRAG | 1 cut(s) | 200 |
| SphI | GCATGC | 1 cut(s) | 340 |
| Sse9I | AATT | 4 cut(s) | 74, 218, 425, 470 |
| SsiI | CCGC | 4 cut(s) | 292, 433, 449, 493 |
| SspMI | CTAG | 1 cut(s) | 496 |
| StyD4I | CCNGG | 1 cut(s) | 165 |
| StyI | CCWWGG | 1 cut(s) | 102 |
| TaiI | ACGT | 1 cut(s) | 378 |
| TaqI | TCGA | 5 cut(s) | 48, 87, 98, 366, 481 |
| TasI | AATT | 4 cut(s) | 74, 218, 425, 470 |
| TatI | WGTACW | 1 cut(s) | 316 |
| TauI | GCSGC | 2 cut(s) | 294, 495 |
| TfiI | GAWTC | 1 cut(s) | 189 |
| TscAI | CASTG | 1 cut(s) | 40 |
| TseI | GCWGC | 1 cut(s) | 120 |
| TspDTI | ATGAA | 2 cut(s) | 126, 211 |
| TspRI | CASTG | 1 cut(s) | 40 |
| Tth111I | GACNNNGTC | 1 cut(s) | 83 |
| VpaK11BI | GGWCC | 2 cut(s) | 261, 383 |
| XapI | RAATTY | 1 cut(s) | 218 |
| XceI | RCATGY | 1 cut(s) | 340 |
| XspI | CTAG | 1 cut(s) | 496 |
| Zsp2I | ATGCAT | 1 cut(s) | 360 |