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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 147, 753 |
| AccB1I | GGYRCC | 1 cut(s) | 503 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 665 |
| AccII | CGCG | 3 cut(s) | 53, 699, 903 |
| AciI | CCGC | 6 cut(s) | 53, 63, 491, 608, 943, 1052 |
| AclWI | GGATC | 3 cut(s) | 101, 650, 953 |
| AcoI | YGGCCR | 2 cut(s) | 72, 465 |
| AcuI | CTGAAG | 3 cut(s) | 342, 588, 753 |
| AcyI | GRCGYC | 1 cut(s) | 504 |
| AfaI | GTAC | 4 cut(s) | 276, 498, 702, 809 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 572, 665, 870, 871 |
| AflII | CTTAAG | 1 cut(s) | 704 |
| AgeI | ACCGGT | 1 cut(s) | 595 |
| AgsI | TTSAA | 3 cut(s) | 428, 632, 986 |
| AjnI | CCWGG | 5 cut(s) | 384, 664, 737, 781, 858 |
| AluBI | AGCT | 4 cut(s) | 315, 635, 695, 731 |
| AluI | AGCT | 4 cut(s) | 315, 635, 695, 731 |
| Alw26I | GTCTC | 2 cut(s) | 12, 930 |
| AlwI | GGATC | 3 cut(s) | 101, 650, 953 |
| ApeKI | GCWGC | 5 cut(s) | 488, 641, 644, 647, 778 |
| AsiGI | ACCGGT | 1 cut(s) | 595 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 609 |
| AspA2I | CCTAGG | 1 cut(s) | 910 |
| AspLEI | GCGC | 5 cut(s) | 226, 355, 374, 400, 506 |
| AspS9I | GGNCC | 9 cut(s) | 9, 156, 179, 217, 283, 668, 862, 874, 1009 |
| AsuC2I | CCSGG | 2 cut(s) | 720, 1012 |
| AsuHPI | GGTGA | 2 cut(s) | 539, 721 |
| AsuNHI | GCTAGC | 2 cut(s) | 691, 923 |
| AvaII | GGWCC | 1 cut(s) | 156 |
| AvrII | CCTAGG | 1 cut(s) | 910 |
| BalI | TGGCCA | 1 cut(s) | 74 |
| BanI | GGYRCC | 1 cut(s) | 503 |
| BanII | GRGCYC | 1 cut(s) | 802 |
| BbvI | GCAGC | 5 cut(s) | 475, 628, 631, 634, 765 |
| BccI | CCATC | 1 cut(s) | 845 |
| BceAI | ACGGC | 2 cut(s) | 452, 1023 |
| BciT130I | CCWGG | 5 cut(s) | 386, 666, 739, 783, 860 |
| BcnI | CCSGG | 2 cut(s) | 720, 1012 |
| BcoDI | GTCTC | 2 cut(s) | 12, 930 |
| BfaI | CTAG | 8 cut(s) | 192, 602, 692, 803, 911, 924, 1049, 1072 |
| BfoI | RGCGCY | 4 cut(s) | 227, 375, 401, 507 |
| BfrI | CTTAAG | 1 cut(s) | 704 |
| BisI | GCNGC | 8 cut(s) | 54, 63, 489, 642, 645, 648, 779, 1053 |
| BlnI | CCTAGG | 1 cut(s) | 910 |
| BlsI | GCNGC | 8 cut(s) | 55, 64, 490, 643, 646, 649, 780, 1054 |
| BmcAI | AGTACT | 2 cut(s) | 498, 809 |
| Bme1390I | CCNGG | 7 cut(s) | 386, 666, 720, 739, 783, 860, 1012 |
| Bme18I | GGWCC | 1 cut(s) | 156 |
| BmgT120I | GGNCC | 9 cut(s) | 9, 156, 179, 217, 283, 668, 862, 874, 1009 |
| BmiI | GGNNCC | 7 cut(s) | 181, 366, 505, 612, 624, 875, 1029 |
| BmrFI | CCNGG | 7 cut(s) | 386, 666, 720, 739, 783, 860, 1012 |
| BmrI | ACTGGG | 2 cut(s) | 180, 287 |
| BmsI | GCATC | 2 cut(s) | 34, 340 |
| BmtI | GCTAGC | 2 cut(s) | 695, 927 |
| BmuI | ACTGGG | 2 cut(s) | 180, 287 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 325, 357 |
| BpmI | CTGGAG | 1 cut(s) | 1058 |
| BpuEI | CTTGAG | 3 cut(s) | 145, 832, 1037 |
| BpuMI | CCSGG | 2 cut(s) | 720, 1012 |
| BsaBI | GATNNNNATC | 1 cut(s) | 446 |
| BsaHI | GRCGYC | 1 cut(s) | 504 |
| BsaJI | CCNNGG | 5 cut(s) | 110, 665, 738, 858, 910 |
| BsaWI | WCCGGW | 1 cut(s) | 595 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 572, 665, 870, 871 |
| Bse118I | RCCGGY | 2 cut(s) | 524, 595 |
| Bse1I | ACTGG | 5 cut(s) | 147, 175, 235, 293, 687 |
| Bse3DI | GCAATG | 2 cut(s) | 261, 483 |
| Bse8I | GATNNNNATC | 1 cut(s) | 446 |
| BseBI | CCWGG | 5 cut(s) | 386, 666, 739, 783, 860 |
| BseDI | CCNNGG | 5 cut(s) | 110, 665, 738, 858, 910 |
| BseGI | GGATG | 4 cut(s) | 127, 355, 588, 788 |
| BseJI | GATNNNNATC | 1 cut(s) | 446 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 572, 665, 870, 871 |
| BseMI | GCAATG | 2 cut(s) | 261, 483 |
| BseMII | CTCAG | 2 cut(s) | 834, 1071 |
| BseNI | ACTGG | 5 cut(s) | 147, 175, 235, 293, 687 |
| BseRI | GAGGAG | 3 cut(s) | 434, 705, 886 |
| BseX3I | CGGCCG | 1 cut(s) | 465 |
| BseXI | GCAGC | 5 cut(s) | 475, 628, 631, 634, 765 |
| BseYI | CCCAGC | 1 cut(s) | 520 |
| BsgI | GTGCAG | 1 cut(s) | 216 |
| Bsh1236I | CGCG | 3 cut(s) | 53, 699, 903 |
| Bsh1285I | CGRYCG | 1 cut(s) | 468 |
| BshNI | GGYRCC | 1 cut(s) | 503 |
| BshTI | ACCGGT | 1 cut(s) | 595 |
| BsiEI | CGRYCG | 1 cut(s) | 468 |
| BsiSI | CCGG | 5 cut(s) | 464, 525, 596, 720, 1012 |
| BslFI | GGGAC | 3 cut(s) | 750, 755, 952 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 572, 665, 870, 871 |
| BsmAI | GTCTC | 2 cut(s) | 12, 930 |
| BsmFI | GGGAC | 3 cut(s) | 750, 755, 952 |
| BsmI | GAATGC | 1 cut(s) | 342 |
| Bsp1286I | GDGCHC | 1 cut(s) | 802 |
| Bsp143I | GATC | 3 cut(s) | 106, 655, 945 |
| Bsp68I | TCGCGA | 1 cut(s) | 903 |
| BspACI | CCGC | 6 cut(s) | 53, 63, 491, 608, 943, 1052 |
| BspCNI | CTCAG | 2 cut(s) | 835, 1070 |
| BspFNI | CGCG | 3 cut(s) | 53, 699, 903 |
| BspHI | TCATGA | 1 cut(s) | 358 |
| BspLI | GGNNCC | 7 cut(s) | 181, 366, 505, 612, 624, 875, 1029 |
| BspOI | GCTAGC | 2 cut(s) | 695, 927 |
| BspPI | GGATC | 3 cut(s) | 101, 650, 953 |
| BspT107I | GGYRCC | 1 cut(s) | 503 |
| BspTI | CTTAAG | 1 cut(s) | 704 |
| BsrDI | GCAATG | 2 cut(s) | 261, 483 |
| BsrFI | RCCGGY | 2 cut(s) | 524, 595 |
| BsrI | ACTGG | 5 cut(s) | 147, 175, 235, 293, 687 |
| BssAI | RCCGGY | 2 cut(s) | 524, 595 |
| BssECI | CCNNGG | 5 cut(s) | 110, 665, 738, 858, 910 |
| BssMI | GATC | 3 cut(s) | 106, 655, 945 |
| BssNI | GRCGYC | 1 cut(s) | 504 |
| BssT1I | CCWWGG | 1 cut(s) | 910 |
| Bst2UI | CCWGG | 5 cut(s) | 386, 666, 739, 783, 860 |
| Bst4CI | ACNGT | 3 cut(s) | 86, 755, 1000 |
| Bst6I | CTCTTC | 2 cut(s) | 111, 324 |
| BstACI | GRCGYC | 1 cut(s) | 504 |
| BstAFI | CTTAAG | 1 cut(s) | 704 |
| BstC8I | GCNNGC | 9 cut(s) | 199, 259, 268, 317, 552, 693, 697, 834, 925 |
| BstDEI | CTNAG | 2 cut(s) | 843, 1057 |
| BstDSI | CCRYGG | 1 cut(s) | 110 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 570 |
| BstF5I | GGATG | 4 cut(s) | 127, 355, 588, 788 |
| BstFNI | CGCG | 3 cut(s) | 53, 699, 903 |
| BstH2I | RGCGCY | 4 cut(s) | 227, 375, 401, 507 |
| BstHHI | GCGC | 5 cut(s) | 226, 355, 374, 400, 506 |
| BstKTI | GATC | 3 cut(s) | 109, 658, 948 |
| BstMAI | GTCTC | 2 cut(s) | 12, 930 |
| BstMBI | GATC | 3 cut(s) | 106, 655, 945 |
| BstMCI | CGRYCG | 1 cut(s) | 468 |
| BstNI | CCWGG | 5 cut(s) | 386, 666, 739, 783, 860 |
| BstNSI | RCATGY | 2 cut(s) | 25, 302 |
| BstSCI | CCNGG | 7 cut(s) | 384, 664, 718, 737, 781, 858, 1010 |
| BstUI | CGCG | 3 cut(s) | 53, 699, 903 |
| BstV1I | GCAGC | 5 cut(s) | 475, 628, 631, 634, 765 |
| BstX2I | RGATCY | 1 cut(s) | 655 |
| BstYI | RGATCY | 1 cut(s) | 655 |
| BstZI | CGGCCG | 1 cut(s) | 465 |
| BtgI | CCRYGG | 1 cut(s) | 110 |
| BtsCI | GGATG | 4 cut(s) | 127, 355, 588, 788 |
| BtsI | GCAGTG | 1 cut(s) | 659 |
| BtsIMutI | CAGTG | 5 cut(s) | 102, 228, 300, 659, 840 |
| BtuMI | TCGCGA | 1 cut(s) | 903 |
| Cac8I | GCNNGC | 9 cut(s) | 199, 259, 268, 317, 552, 693, 697, 834, 925 |
| CciI | TCATGA | 1 cut(s) | 358 |
| CfoI | GCGC | 5 cut(s) | 226, 355, 374, 400, 506 |
| Cfr10I | RCCGGY | 2 cut(s) | 524, 595 |
| Cfr13I | GGNCC | 9 cut(s) | 9, 156, 179, 217, 283, 668, 862, 874, 1009 |
| Csp6I | GTAC | 4 cut(s) | 275, 497, 701, 808 |
| CspAI | ACCGGT | 1 cut(s) | 595 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 217, 252 |
| CviAII | CATG | 5 cut(s) | 22, 299, 359, 455, 1022 |
| CviQI | GTAC | 4 cut(s) | 275, 497, 701, 808 |
| DdeI | CTNAG | 2 cut(s) | 843, 1057 |
| DinI | GGCGCC | 1 cut(s) | 505 |
| DpnI | GATC | 3 cut(s) | 108, 657, 947 |
| DpnII | GATC | 3 cut(s) | 106, 655, 945 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 147, 753 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 147, 753 |
| EaeI | YGGCCR | 2 cut(s) | 72, 465 |
| EagI | CGGCCG | 1 cut(s) | 465 |
| Eam1104I | CTCTTC | 2 cut(s) | 111, 324 |
| EarI | CTCTTC | 2 cut(s) | 111, 324 |
| EclXI | CGGCCG | 1 cut(s) | 465 |
| Eco130I | CCWWGG | 1 cut(s) | 910 |
| Eco24I | GRGCYC | 1 cut(s) | 802 |
| Eco32I | GATATC | 1 cut(s) | 772 |
| Eco47I | GGWCC | 1 cut(s) | 156 |
| Eco52I | CGGCCG | 1 cut(s) | 465 |
| Eco57I | CTGAAG | 3 cut(s) | 342, 588, 753 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 570 |
| EcoO109I | RGGNCCY | 3 cut(s) | 156, 179, 874 |
| EcoRII | CCWGG | 5 cut(s) | 384, 664, 737, 781, 858 |
| EcoRV | GATATC | 1 cut(s) | 772 |
| EcoT14I | CCWWGG | 1 cut(s) | 910 |
| EcoT22I | ATGCAT | 1 cut(s) | 27 |
| EcoT38I | GRGCYC | 1 cut(s) | 802 |
| EgeI | GGCGCC | 1 cut(s) | 505 |
| EheI | GGCGCC | 1 cut(s) | 505 |
| ErhI | CCWWGG | 1 cut(s) | 910 |
| FaeI | CATG | 5 cut(s) | 25, 302, 362, 458, 1025 |
| FaiI | YATR | 9 cut(s) | 23, 40, 153, 300, 305, 360, 456, 728, 1023 |
| FaqI | GGGAC | 3 cut(s) | 750, 755, 952 |
| FatI | CATG | 5 cut(s) | 21, 298, 358, 454, 1021 |
| Fnu4HI | GCNGC | 8 cut(s) | 54, 63, 489, 642, 645, 648, 779, 1053 |
| FokI | GGATG | 4 cut(s) | 134, 362, 595, 775 |
| FriOI | GRGCYC | 1 cut(s) | 802 |
| Fsp4HI | GCNGC | 8 cut(s) | 54, 63, 489, 642, 645, 648, 779, 1053 |
| FspBI | CTAG | 8 cut(s) | 192, 602, 692, 803, 911, 924, 1049, 1072 |
| GlaI | GCGC | 5 cut(s) | 225, 354, 373, 399, 505 |
| GluI | GCNGC | 8 cut(s) | 54, 63, 489, 642, 645, 648, 779, 1053 |
| GsaI | CCCAGC | 1 cut(s) | 524 |
| GsuI | CTGGAG | 1 cut(s) | 1058 |
| HaeII | RGCGCY | 4 cut(s) | 227, 375, 401, 507 |
| HapII | CCGG | 5 cut(s) | 464, 525, 596, 720, 1012 |
| HhaI | GCGC | 5 cut(s) | 226, 355, 374, 400, 506 |
| Hin1I | GRCGYC | 1 cut(s) | 504 |
| Hin1II | CATG | 5 cut(s) | 25, 302, 362, 458, 1025 |
| Hin6I | GCGC | 5 cut(s) | 224, 353, 372, 398, 504 |
| HinP1I | GCGC | 5 cut(s) | 224, 353, 372, 398, 504 |
| HincII | GTYRAC | 1 cut(s) | 541 |
| HindII | GTYRAC | 1 cut(s) | 541 |
| HinfI | GANTC | 4 cut(s) | 712, 747, 763, 893 |
| HpaI | GTTAAC | 1 cut(s) | 541 |
| HpaII | CCGG | 5 cut(s) | 464, 525, 596, 720, 1012 |
| HphI | GGTGA | 2 cut(s) | 539, 721 |
| Hpy166II | GTNNAC | 1 cut(s) | 541 |
| Hpy188I | TCNGA | 3 cut(s) | 94, 349, 1060 |
| Hpy8I | GTNNAC | 1 cut(s) | 541 |
| Hpy99I | CGWCG | 1 cut(s) | 797 |
| HpyAV | CCTTC | 2 cut(s) | 170, 684 |
| HpyCH4III | ACNGT | 3 cut(s) | 86, 755, 1000 |
| HpyCH4IV | ACGT | 1 cut(s) | 273 |
| HpyCH4V | TGCA | 3 cut(s) | 25, 197, 298 |
| HpyF3I | CTNAG | 2 cut(s) | 843, 1057 |
| HpySE526I | ACGT | 1 cut(s) | 273 |
| Hsp92I | GRCGYC | 1 cut(s) | 504 |
| Hsp92II | CATG | 5 cut(s) | 25, 302, 362, 458, 1025 |
| HspAI | GCGC | 5 cut(s) | 224, 353, 372, 398, 504 |
| KasI | GGCGCC | 1 cut(s) | 503 |
| KspAI | GTTAAC | 1 cut(s) | 541 |
| Kzo9I | GATC | 3 cut(s) | 106, 655, 945 |
| LmnI | GCTCC | 5 cut(s) | 294, 568, 622, 640, 1039 |
| Lsp1109I | GCAGC | 5 cut(s) | 475, 628, 631, 634, 765 |
| LweI | GCATC | 2 cut(s) | 34, 340 |
| MaeI | CTAG | 8 cut(s) | 192, 602, 692, 803, 911, 924, 1049, 1072 |
| MaeII | ACGT | 1 cut(s) | 273 |
| MaeIII | GTNAC | 2 cut(s) | 709, 1003 |
| MalI | GATC | 3 cut(s) | 108, 657, 947 |
| MboI | GATC | 3 cut(s) | 106, 655, 945 |
| MboII | GAAGA | 5 cut(s) | 128, 341, 542, 812, 815 |
| MfeI | CAATTG | 2 cut(s) | 278, 992 |
| MflI | RGATCY | 1 cut(s) | 655 |
| MhlI | GDGCHC | 1 cut(s) | 802 |
| MlsI | TGGCCA | 1 cut(s) | 74 |
| MluCI | AATT | 3 cut(s) | 278, 408, 992 |
| MluNI | TGGCCA | 1 cut(s) | 74 |
| Mly113I | GGCGCC | 1 cut(s) | 504 |
| MlyI | GAGTC | 4 cut(s) | 706, 741, 772, 887 |
| Mox20I | TGGCCA | 1 cut(s) | 74 |
| Mph1103I | ATGCAT | 1 cut(s) | 27 |
| MroXI | GAANNNNTTC | 1 cut(s) | 609 |
| MscI | TGGCCA | 1 cut(s) | 74 |
| MseI | TTAA | 3 cut(s) | 78, 540, 705 |
| MslI | CAYNNNNRTG | 1 cut(s) | 348 |
| Msp20I | TGGCCA | 1 cut(s) | 74 |
| MspCI | CTTAAG | 1 cut(s) | 704 |
| MspI | CCGG | 5 cut(s) | 464, 525, 596, 720, 1012 |
| MspR9I | CCNGG | 7 cut(s) | 386, 666, 720, 739, 783, 860, 1012 |
| MunI | CAATTG | 2 cut(s) | 278, 992 |
| Mva1269I | GAATGC | 1 cut(s) | 342 |
| MvaI | CCWGG | 5 cut(s) | 386, 666, 739, 783, 860 |
| MvnI | CGCG | 3 cut(s) | 53, 699, 903 |
| NarI | GGCGCC | 1 cut(s) | 504 |
| NciI | CCSGG | 2 cut(s) | 720, 1012 |
| NdeII | GATC | 3 cut(s) | 106, 655, 945 |
| NheI | GCTAGC | 2 cut(s) | 691, 923 |
| NlaIII | CATG | 5 cut(s) | 25, 302, 362, 458, 1025 |
| NlaIV | GGNNCC | 7 cut(s) | 181, 366, 505, 612, 624, 875, 1029 |
| NmuCI | GTSAC | 2 cut(s) | 709, 1003 |
| NruI | TCGCGA | 1 cut(s) | 903 |
| NsiI | ATGCAT | 1 cut(s) | 27 |
| NspI | RCATGY | 2 cut(s) | 25, 302 |
| PagI | TCATGA | 1 cut(s) | 358 |
| PctI | GAATGC | 1 cut(s) | 342 |
| PdmI | GAANNNNTTC | 1 cut(s) | 609 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 665 |
| PfoI | TCCNGGA | 1 cut(s) | 718 |
| PinAI | ACCGGT | 1 cut(s) | 595 |
| PkrI | GCNGC | 8 cut(s) | 55, 64, 490, 643, 646, 649, 780, 1054 |
| PleI | GAGTC | 4 cut(s) | 706, 741, 771, 887 |
| PluTI | GGCGCC | 1 cut(s) | 507 |
| PpsI | GAGTC | 4 cut(s) | 706, 741, 771, 887 |
| PpuMI | RGGWCCY | 1 cut(s) | 156 |
| Psp5II | RGGWCCY | 1 cut(s) | 156 |
| Psp6I | CCWGG | 5 cut(s) | 384, 664, 737, 781, 858 |
| PspFI | CCCAGC | 1 cut(s) | 520 |
| PspGI | CCWGG | 5 cut(s) | 384, 664, 737, 781, 858 |
| PspN4I | GGNNCC | 7 cut(s) | 181, 366, 505, 612, 624, 875, 1029 |
| PspPI | GGNCC | 9 cut(s) | 9, 156, 179, 217, 283, 668, 862, 874, 1009 |
| PspPPI | RGGWCCY | 1 cut(s) | 156 |
| PsuI | RGATCY | 1 cut(s) | 655 |
| RruI | TCGCGA | 1 cut(s) | 903 |
| RsaI | GTAC | 4 cut(s) | 276, 498, 702, 809 |
| RsaNI | GTAC | 4 cut(s) | 275, 497, 701, 808 |
| RseI | CAYNNNNRTG | 1 cut(s) | 348 |
| SaqAI | TTAA | 3 cut(s) | 78, 540, 705 |
| SatI | GCNGC | 8 cut(s) | 54, 63, 489, 642, 645, 648, 779, 1053 |
| Sau3AI | GATC | 3 cut(s) | 106, 655, 945 |
| Sau96I | GGNCC | 9 cut(s) | 9, 156, 179, 217, 283, 668, 862, 874, 1009 |
| ScaI | AGTACT | 2 cut(s) | 498, 809 |
| SchI | GAGTC | 4 cut(s) | 706, 741, 772, 887 |
| ScrFI | CCNGG | 7 cut(s) | 386, 666, 720, 739, 783, 860, 1012 |
| SduI | GDGCHC | 1 cut(s) | 802 |
| SetI | ASST | 9 cut(s) | 158, 253, 276, 317, 426, 637, 697, 711, 733 |
| SfaNI | GCATC | 2 cut(s) | 34, 340 |
| SfoI | GGCGCC | 1 cut(s) | 505 |
| SinI | GGWCC | 1 cut(s) | 156 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 348 |
| SmlI | CTYRAG | 4 cut(s) | 160, 704, 811, 1016 |
| SmoI | CTYRAG | 4 cut(s) | 160, 704, 811, 1016 |
| Sse9I | AATT | 3 cut(s) | 278, 408, 992 |
| SsiI | CCGC | 6 cut(s) | 53, 63, 491, 608, 943, 1052 |
| SspDI | GGCGCC | 1 cut(s) | 503 |
| SspI | AATATT | 1 cut(s) | 1065 |
| SspMI | CTAG | 8 cut(s) | 192, 602, 692, 803, 911, 924, 1049, 1072 |
| StyD4I | CCNGG | 7 cut(s) | 384, 664, 718, 737, 781, 858, 1010 |
| StyI | CCWWGG | 1 cut(s) | 910 |
| TaaI | ACNGT | 3 cut(s) | 86, 755, 1000 |
| TaiI | ACGT | 1 cut(s) | 276 |
| TaqI | TCGA | 2 cut(s) | 591, 826 |
| TasI | AATT | 3 cut(s) | 278, 408, 992 |
| TatI | WGTACW | 2 cut(s) | 496, 807 |
| TauI | GCSGC | 3 cut(s) | 56, 65, 1055 |
| Tru1I | TTAA | 3 cut(s) | 78, 540, 705 |
| Tru9I | TTAA | 3 cut(s) | 78, 540, 705 |
| TscAI | CASTG | 5 cut(s) | 109, 235, 300, 666, 847 |
| TseFI | GTSAC | 2 cut(s) | 709, 1003 |
| TseI | GCWGC | 5 cut(s) | 488, 641, 644, 647, 778 |
| Tsp45I | GTSAC | 2 cut(s) | 709, 1003 |
| TspDTI | ATGAA | 3 cut(s) | 138, 599, 864 |
| TspGWI | ACGGA | 3 cut(s) | 99, 759, 781 |
| TspRI | CASTG | 5 cut(s) | 109, 235, 300, 666, 847 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 665 |
| Vha464I | CTTAAG | 1 cut(s) | 704 |
| VpaK11BI | GGWCC | 1 cut(s) | 156 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 570 |
| XbaI | TCTAGA | 1 cut(s) | 802 |
| XceI | RCATGY | 2 cut(s) | 25, 302 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 227, 382 |
| XmaJI | CCTAGG | 1 cut(s) | 910 |
| XmnI | GAANNNNTTC | 1 cut(s) | 609 |
| XspI | CTAG | 8 cut(s) | 192, 602, 692, 803, 911, 924, 1049, 1072 |
| ZrmI | AGTACT | 2 cut(s) | 498, 809 |
| Zsp2I | ATGCAT | 1 cut(s) | 27 |