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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 731 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 532 |
| AccBSI | CCGCTC | 1 cut(s) | 259 |
| AccII | CGCG | 5 cut(s) | 126, 128, 255, 283, 386 |
| AccIII | TCCGGA | 1 cut(s) | 676 |
| AciI | CCGC | 7 cut(s) | 89, 124, 253, 259, 338, 386, 490 |
| AcoI | YGGCCR | 1 cut(s) | 357 |
| AcuI | CTGAAG | 1 cut(s) | 708 |
| AcyI | GRCGYC | 1 cut(s) | 732 |
| AdeI | CACNNNGTG | 1 cut(s) | 212 |
| AfaI | GTAC | 3 cut(s) | 36, 236, 246 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 23, 187, 259, 532, 580, 676 |
| AflIII | ACRYGT | 1 cut(s) | 281 |
| AgsI | TTSAA | 2 cut(s) | 134, 422 |
| AjnI | CCWGG | 7 cut(s) | 22, 145, 345, 531, 567, 633, 772 |
| AluBI | AGCT | 1 cut(s) | 382 |
| AluI | AGCT | 1 cut(s) | 382 |
| Alw21I | GWGCWC | 1 cut(s) | 384 |
| Ama87I | CYCGRG | 1 cut(s) | 648 |
| Aor13HI | TCCGGA | 1 cut(s) | 676 |
| AoxI | GGCC | 8 cut(s) | 120, 225, 357, 463, 513, 570, 582, 590 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 395 |
| AspLEI | GCGC | 3 cut(s) | 128, 130, 734 |
| AspS9I | GGNCC | 7 cut(s) | 121, 191, 225, 464, 514, 523, 571 |
| AsuC2I | CCSGG | 2 cut(s) | 210, 296 |
| AsuHPI | GGTGA | 2 cut(s) | 242, 362 |
| AvaI | CYCGRG | 1 cut(s) | 648 |
| AvaII | GGWCC | 2 cut(s) | 191, 523 |
| BanI | GGYRCC | 1 cut(s) | 731 |
| BanII | GRGCYC | 1 cut(s) | 384 |
| BbsI | GAAGAC | 1 cut(s) | 707 |
| Bbv12I | GWGCWC | 1 cut(s) | 384 |
| BccI | CCATC | 3 cut(s) | 320, 370, 632 |
| BceAI | ACGGC | 1 cut(s) | 165 |
| BciT130I | CCWGG | 7 cut(s) | 24, 147, 347, 533, 569, 635, 774 |
| BcnI | CCSGG | 2 cut(s) | 210, 296 |
| BfaI | CTAG | 2 cut(s) | 431, 495 |
| BfoI | RGCGCY | 1 cut(s) | 735 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 479 |
| BglII | AGATCT | 1 cut(s) | 415 |
| BmcAI | AGTACT | 1 cut(s) | 246 |
| Bme1390I | CCNGG | 9 cut(s) | 24, 147, 210, 296, 347, 533, 569, 635, 774 |
| Bme18I | GGWCC | 2 cut(s) | 191, 523 |
| BmeT110I | CYCGRG | 1 cut(s) | 648 |
| BmgT120I | GGNCC | 7 cut(s) | 121, 191, 225, 464, 514, 523, 571 |
| BmiI | GGNNCC | 4 cut(s) | 63, 192, 668, 733 |
| BmrFI | CCNGG | 9 cut(s) | 24, 147, 210, 296, 347, 533, 569, 635, 774 |
| BpiI | GAAGAC | 1 cut(s) | 707 |
| BpuEI | CTTGAG | 3 cut(s) | 77, 540, 730 |
| BpuMI | CCSGG | 2 cut(s) | 210, 296 |
| BsaHI | GRCGYC | 1 cut(s) | 732 |
| BsaJI | CCNNGG | 4 cut(s) | 23, 346, 531, 567 |
| BsaWI | WCCGGW | 1 cut(s) | 676 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 23, 187, 259, 532, 580, 676 |
| Bse1I | ACTGG | 1 cut(s) | 483 |
| Bse3DI | GCAATG | 1 cut(s) | 466 |
| BseAI | TCCGGA | 1 cut(s) | 676 |
| BseBI | CCWGG | 7 cut(s) | 24, 147, 347, 533, 569, 635, 774 |
| BseDI | CCNNGG | 4 cut(s) | 23, 346, 531, 567 |
| BseGI | GGATG | 3 cut(s) | 166, 630, 643 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 23, 187, 259, 532, 580, 676 |
| BseMI | GCAATG | 1 cut(s) | 466 |
| BseMII | CTCAG | 3 cut(s) | 261, 613, 757 |
| BseNI | ACTGG | 1 cut(s) | 483 |
| BsePI | GCGCGC | 1 cut(s) | 126 |
| BseYI | CCCAGC | 1 cut(s) | 187 |
| Bsh1236I | CGCG | 5 cut(s) | 126, 128, 255, 283, 386 |
| BshFI | GGCC | 8 cut(s) | 122, 227, 359, 465, 515, 572, 584, 592 |
| BshNI | GGYRCC | 1 cut(s) | 731 |
| BsiHKAI | GWGCWC | 1 cut(s) | 384 |
| BsiHKCI | CYCGRG | 1 cut(s) | 648 |
| BsiSI | CCGG | 3 cut(s) | 210, 296, 677 |
| BslFI | GGGAC | 1 cut(s) | 550 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 23, 187, 259, 532, 580, 676 |
| BsmFI | GGGAC | 1 cut(s) | 550 |
| BsmI | GAATGC | 1 cut(s) | 611 |
| BsnI | GGCC | 8 cut(s) | 122, 227, 359, 465, 515, 572, 584, 592 |
| BsoBI | CYCGRG | 1 cut(s) | 648 |
| Bsp1286I | GDGCHC | 1 cut(s) | 384 |
| Bsp13I | TCCGGA | 1 cut(s) | 676 |
| Bsp143I | GATC | 1 cut(s) | 415 |
| BspACI | CCGC | 7 cut(s) | 89, 124, 253, 259, 338, 386, 490 |
| BspANI | GGCC | 8 cut(s) | 122, 227, 359, 465, 515, 572, 584, 592 |
| BspCNI | CTCAG | 3 cut(s) | 262, 612, 756 |
| BspEI | TCCGGA | 1 cut(s) | 676 |
| BspFNI | CGCG | 5 cut(s) | 126, 128, 255, 283, 386 |
| BspLI | GGNNCC | 4 cut(s) | 63, 192, 668, 733 |
| BspT107I | GGYRCC | 1 cut(s) | 731 |
| BsrBI | CCGCTC | 1 cut(s) | 259 |
| BsrDI | GCAATG | 1 cut(s) | 466 |
| BsrI | ACTGG | 1 cut(s) | 483 |
| BssECI | CCNNGG | 4 cut(s) | 23, 346, 531, 567 |
| BssHII | GCGCGC | 1 cut(s) | 126 |
| BssMI | GATC | 1 cut(s) | 415 |
| BssNI | GRCGYC | 1 cut(s) | 732 |
| Bst2UI | CCWGG | 7 cut(s) | 24, 147, 347, 533, 569, 635, 774 |
| Bst4CI | ACNGT | 1 cut(s) | 607 |
| Bst6I | CTCTTC | 3 cut(s) | 147, 573, 728 |
| BstACI | GRCGYC | 1 cut(s) | 732 |
| BstC8I | GCNNGC | 7 cut(s) | 120, 124, 128, 257, 261, 384, 492 |
| BstDEI | CTNAG | 4 cut(s) | 229, 270, 599, 743 |
| BstF5I | GGATG | 3 cut(s) | 166, 630, 643 |
| BstFNI | CGCG | 5 cut(s) | 126, 128, 255, 283, 386 |
| BstH2I | RGCGCY | 1 cut(s) | 735 |
| BstHHI | GCGC | 3 cut(s) | 128, 130, 734 |
| BstKTI | GATC | 1 cut(s) | 418 |
| BstMBI | GATC | 1 cut(s) | 415 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 68, 431, 479, 500 |
| BstNI | CCWGG | 7 cut(s) | 24, 147, 347, 533, 569, 635, 774 |
| BstSCI | CCNGG | 9 cut(s) | 22, 145, 208, 294, 345, 531, 567, 633, 772 |
| BstUI | CGCG | 5 cut(s) | 126, 128, 255, 283, 386 |
| BstV2I | GAAGAC | 1 cut(s) | 707 |
| BstX2I | RGATCY | 1 cut(s) | 415 |
| BstYI | RGATCY | 1 cut(s) | 415 |
| BsuRI | GGCC | 8 cut(s) | 122, 227, 359, 465, 515, 572, 584, 592 |
| BtsCI | GGATG | 3 cut(s) | 166, 630, 643 |
| BtsI | GCAGTG | 1 cut(s) | 516 |
| BtsIMutI | CAGTG | 2 cut(s) | 267, 516 |
| Cac8I | GCNNGC | 7 cut(s) | 120, 124, 128, 257, 261, 384, 492 |
| CfoI | GCGC | 3 cut(s) | 128, 130, 734 |
| Cfr13I | GGNCC | 7 cut(s) | 121, 191, 225, 464, 514, 523, 571 |
| CseI | GACGC | 2 cut(s) | 272, 289 |
| Csp6I | GTAC | 3 cut(s) | 35, 235, 245 |
| CviAII | CATG | 5 cut(s) | 9, 370, 456, 506, 672 |
| CviQI | GTAC | 3 cut(s) | 35, 235, 245 |
| DdeI | CTNAG | 4 cut(s) | 229, 270, 599, 743 |
| DinI | GGCGCC | 1 cut(s) | 733 |
| DpnI | GATC | 1 cut(s) | 417 |
| DpnII | GATC | 1 cut(s) | 415 |
| DraIII | CACNNNGTG | 1 cut(s) | 212 |
| EaeI | YGGCCR | 1 cut(s) | 357 |
| Eam1104I | CTCTTC | 3 cut(s) | 147, 573, 728 |
| EarI | CTCTTC | 3 cut(s) | 147, 573, 728 |
| Ecl136II | GAGCTC | 1 cut(s) | 382 |
| Eco147I | AGGCCT | 1 cut(s) | 584 |
| Eco24I | GRGCYC | 1 cut(s) | 384 |
| Eco47I | GGWCC | 2 cut(s) | 191, 523 |
| Eco53kI | GAGCTC | 1 cut(s) | 382 |
| Eco57I | CTGAAG | 1 cut(s) | 708 |
| Eco88I | CYCGRG | 1 cut(s) | 648 |
| EcoICRI | GAGCTC | 1 cut(s) | 382 |
| EcoO109I | RGGNCCY | 2 cut(s) | 191, 225 |
| EcoRII | CCWGG | 7 cut(s) | 22, 145, 345, 531, 567, 633, 772 |
| EcoT38I | GRGCYC | 1 cut(s) | 384 |
| EgeI | GGCGCC | 1 cut(s) | 733 |
| EheI | GGCGCC | 1 cut(s) | 733 |
| FaeI | CATG | 5 cut(s) | 12, 373, 459, 509, 675 |
| FaiI | YATR | 7 cut(s) | 10, 221, 371, 457, 507, 589, 673 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 692, 724 |
| FaqI | GGGAC | 1 cut(s) | 550 |
| FatI | CATG | 5 cut(s) | 8, 369, 455, 505, 671 |
| FauI | CCCGC | 4 cut(s) | 131, 252, 331, 483 |
| FokI | GGATG | 3 cut(s) | 173, 617, 650 |
| FriOI | GRGCYC | 1 cut(s) | 384 |
| FspBI | CTAG | 2 cut(s) | 431, 495 |
| GlaI | GCGC | 3 cut(s) | 127, 129, 733 |
| GsaI | CCCAGC | 1 cut(s) | 191 |
| HaeII | RGCGCY | 1 cut(s) | 735 |
| HaeIII | GGCC | 8 cut(s) | 122, 227, 359, 465, 515, 572, 584, 592 |
| HapII | CCGG | 3 cut(s) | 210, 296, 677 |
| HgaI | GACGC | 2 cut(s) | 272, 289 |
| HhaI | GCGC | 3 cut(s) | 128, 130, 734 |
| Hin1I | GRCGYC | 1 cut(s) | 732 |
| Hin1II | CATG | 5 cut(s) | 12, 373, 459, 509, 675 |
| Hin6I | GCGC | 3 cut(s) | 126, 128, 732 |
| HinP1I | GCGC | 3 cut(s) | 126, 128, 732 |
| HinfI | GANTC | 3 cut(s) | 353, 391, 642 |
| HpaII | CCGG | 3 cut(s) | 210, 296, 677 |
| HphI | GGTGA | 2 cut(s) | 242, 362 |
| Hpy188I | TCNGA | 5 cut(s) | 242, 288, 544, 628, 757 |
| Hpy188III | TCNNGA | 5 cut(s) | 56, 194, 557, 601, 677 |
| HpyAV | CCTTC | 3 cut(s) | 111, 164, 686 |
| HpyCH4III | ACNGT | 1 cut(s) | 607 |
| HpyCH4V | TGCA | 3 cut(s) | 215, 459, 509 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 68, 431, 479, 500 |
| HpyF3I | CTNAG | 4 cut(s) | 229, 270, 599, 743 |
| Hsp92I | GRCGYC | 1 cut(s) | 732 |
| Hsp92II | CATG | 5 cut(s) | 12, 373, 459, 509, 675 |
| HspAI | GCGC | 3 cut(s) | 126, 128, 732 |
| KasI | GGCGCC | 1 cut(s) | 731 |
| Kpn2I | TCCGGA | 1 cut(s) | 676 |
| Kzo9I | GATC | 1 cut(s) | 415 |
| LmnI | GCTCC | 3 cut(s) | 67, 379, 679 |
| MaeI | CTAG | 2 cut(s) | 431, 495 |
| MaeIII | GTNAC | 2 cut(s) | 11, 350 |
| MalI | GATC | 1 cut(s) | 417 |
| MbiI | CCGCTC | 1 cut(s) | 259 |
| MboI | GATC | 1 cut(s) | 415 |
| MboII | GAAGA | 9 cut(s) | 41, 134, 410, 590, 701, 709, 712, 715, 718 |
| MfeI | CAATTG | 2 cut(s) | 468, 518 |
| MflI | RGATCY | 1 cut(s) | 415 |
| MhlI | GDGCHC | 1 cut(s) | 384 |
| MluCI | AATT | 3 cut(s) | 107, 468, 518 |
| MluI | ACGCGT | 1 cut(s) | 281 |
| Mly113I | GGCGCC | 1 cut(s) | 732 |
| MlyI | GAGTC | 1 cut(s) | 347 |
| MroI | TCCGGA | 1 cut(s) | 676 |
| MroXI | GAANNNNTTC | 1 cut(s) | 395 |
| MslI | CAYNNNNRTG | 1 cut(s) | 374 |
| MspI | CCGG | 3 cut(s) | 210, 296, 677 |
| MspR9I | CCNGG | 9 cut(s) | 24, 147, 210, 296, 347, 533, 569, 635, 774 |
| MunI | CAATTG | 2 cut(s) | 468, 518 |
| Mva1269I | GAATGC | 1 cut(s) | 611 |
| MvaI | CCWGG | 7 cut(s) | 24, 147, 347, 533, 569, 635, 774 |
| MvnI | CGCG | 5 cut(s) | 126, 128, 255, 283, 386 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 68, 431, 479, 500 |
| NarI | GGCGCC | 1 cut(s) | 732 |
| NciI | CCSGG | 2 cut(s) | 210, 296 |
| NdeII | GATC | 1 cut(s) | 415 |
| NlaIII | CATG | 5 cut(s) | 12, 373, 459, 509, 675 |
| NlaIV | GGNNCC | 4 cut(s) | 63, 192, 668, 733 |
| NmeAIII | GCCGAG | 2 cut(s) | 314, 335 |
| NmuCI | GTSAC | 1 cut(s) | 350 |
| PauI | GCGCGC | 1 cut(s) | 126 |
| PceI | AGGCCT | 1 cut(s) | 584 |
| PctI | GAATGC | 1 cut(s) | 611 |
| PdmI | GAANNNNTTC | 1 cut(s) | 395 |
| PfeI | GAWTC | 2 cut(s) | 391, 642 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 532 |
| PfoI | TCCNGGA | 3 cut(s) | 145, 633, 772 |
| PleI | GAGTC | 1 cut(s) | 347 |
| PluTI | GGCGCC | 1 cut(s) | 735 |
| PpsI | GAGTC | 1 cut(s) | 347 |
| PpuMI | RGGWCCY | 1 cut(s) | 191 |
| Psp124BI | GAGCTC | 1 cut(s) | 384 |
| Psp5II | RGGWCCY | 1 cut(s) | 191 |
| Psp6I | CCWGG | 7 cut(s) | 22, 145, 345, 531, 567, 633, 772 |
| PspFI | CCCAGC | 1 cut(s) | 187 |
| PspGI | CCWGG | 7 cut(s) | 22, 145, 345, 531, 567, 633, 772 |
| PspN4I | GGNNCC | 4 cut(s) | 63, 192, 668, 733 |
| PspPI | GGNCC | 7 cut(s) | 121, 191, 225, 464, 514, 523, 571 |
| PspPPI | RGGWCCY | 1 cut(s) | 191 |
| PsuI | RGATCY | 1 cut(s) | 415 |
| PteI | GCGCGC | 1 cut(s) | 126 |
| RsaI | GTAC | 3 cut(s) | 36, 236, 246 |
| RsaNI | GTAC | 3 cut(s) | 35, 235, 245 |
| RseI | CAYNNNNRTG | 1 cut(s) | 374 |
| SacI | GAGCTC | 1 cut(s) | 384 |
| Sau3AI | GATC | 1 cut(s) | 415 |
| Sau96I | GGNCC | 7 cut(s) | 121, 191, 225, 464, 514, 523, 571 |
| ScaI | AGTACT | 1 cut(s) | 246 |
| SchI | GAGTC | 1 cut(s) | 347 |
| ScrFI | CCNGG | 9 cut(s) | 24, 147, 210, 296, 347, 533, 569, 635, 774 |
| SduI | GDGCHC | 1 cut(s) | 384 |
| SetI | ASST | 7 cut(s) | 40, 175, 240, 384, 710, 742, 768 |
| SfoI | GGCGCC | 1 cut(s) | 733 |
| SinI | GGWCC | 2 cut(s) | 191, 523 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 374 |
| SmlI | CTYRAG | 3 cut(s) | 56, 555, 709 |
| SmoI | CTYRAG | 3 cut(s) | 56, 555, 709 |
| Sse9I | AATT | 3 cut(s) | 107, 468, 518 |
| SseBI | AGGCCT | 1 cut(s) | 584 |
| SsiI | CCGC | 7 cut(s) | 89, 124, 253, 259, 338, 386, 490 |
| SspDI | GGCGCC | 1 cut(s) | 731 |
| SspMI | CTAG | 2 cut(s) | 431, 495 |
| SstI | GAGCTC | 1 cut(s) | 384 |
| StuI | AGGCCT | 1 cut(s) | 584 |
| StyD4I | CCNGG | 9 cut(s) | 22, 145, 208, 294, 345, 531, 567, 633, 772 |
| TaaI | ACNGT | 1 cut(s) | 607 |
| TasI | AATT | 3 cut(s) | 107, 468, 518 |
| TatI | WGTACW | 1 cut(s) | 244 |
| TfiI | GAWTC | 2 cut(s) | 391, 642 |
| TscAI | CASTG | 2 cut(s) | 274, 516 |
| TseFI | GTSAC | 1 cut(s) | 350 |
| Tsp45I | GTSAC | 1 cut(s) | 350 |
| TspGWI | ACGGA | 1 cut(s) | 758 |
| TspRI | CASTG | 2 cut(s) | 274, 516 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 532 |
| VpaK11BI | GGWCC | 2 cut(s) | 191, 523 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 367 |
| XmnI | GAANNNNTTC | 1 cut(s) | 395 |
| XspI | CTAG | 2 cut(s) | 431, 495 |
| ZrmI | AGTACT | 1 cut(s) | 246 |