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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 29 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 243 |
| Acc36I | ACCTGC | 3 cut(s) | 25, 29, 403 |
| AccII | CGCG | 8 cut(s) | 180, 209, 309, 371, 373, 395, 432, 462 |
| AclWI | GGATC | 3 cut(s) | 15, 504, 512 |
| AcsI | RAATTY | 1 cut(s) | 332 |
| AcuI | CTGAAG | 1 cut(s) | 153 |
| AfaI | GTAC | 4 cut(s) | 60, 183, 300, 316 |
| AfeI | AGCGCT | 1 cut(s) | 483 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 38, 98, 535 |
| AgsI | TTSAA | 1 cut(s) | 341 |
| AjnI | CCWGG | 3 cut(s) | 24, 199, 240 |
| AluBI | AGCT | 3 cut(s) | 272, 281, 352 |
| AluI | AGCT | 3 cut(s) | 272, 281, 352 |
| Alw21I | GWGCWC | 2 cut(s) | 283, 354 |
| AlwI | GGATC | 3 cut(s) | 15, 504, 512 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 44 |
| Aor51HI | AGCGCT | 1 cut(s) | 483 |
| AoxI | GGCC | 4 cut(s) | 100, 433, 567, 588 |
| ApeKI | GCWGC | 5 cut(s) | 107, 110, 232, 269, 398 |
| ApoI | RAATTY | 1 cut(s) | 332 |
| AspA2I | CCTAGG | 1 cut(s) | 469 |
| AspLEI | GCGC | 3 cut(s) | 373, 484, 601 |
| AspS9I | GGNCC | 6 cut(s) | 76, 245, 525, 538, 568, 589 |
| AsuC2I | CCSGG | 1 cut(s) | 264 |
| AsuHPI | GGTGA | 1 cut(s) | 485 |
| AvaII | GGWCC | 4 cut(s) | 76, 245, 525, 538 |
| AvrII | CCTAGG | 1 cut(s) | 469 |
| BanII | GRGCYC | 3 cut(s) | 283, 354, 612 |
| Bbv12I | GWGCWC | 2 cut(s) | 283, 354 |
| BbvI | GCAGC | 5 cut(s) | 94, 97, 244, 281, 385 |
| BccI | CCATC | 1 cut(s) | 289 |
| BceAI | ACGGC | 1 cut(s) | 603 |
| BciT130I | CCWGG | 3 cut(s) | 26, 201, 242 |
| BcnI | CCSGG | 1 cut(s) | 264 |
| BfaI | CTAG | 2 cut(s) | 185, 470 |
| BfmI | CTRYAG | 1 cut(s) | 230 |
| BfoI | RGCGCY | 1 cut(s) | 485 |
| BfuAI | ACCTGC | 3 cut(s) | 25, 29, 403 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 596 |
| BisI | GCNGC | 8 cut(s) | 108, 111, 233, 270, 307, 396, 399, 433 |
| BlnI | CCTAGG | 1 cut(s) | 469 |
| BlsI | GCNGC | 8 cut(s) | 109, 112, 234, 271, 308, 397, 400, 434 |
| Bme1390I | CCNGG | 4 cut(s) | 26, 201, 242, 264 |
| Bme18I | GGWCC | 4 cut(s) | 76, 245, 525, 538 |
| BmgT120I | GGNCC | 6 cut(s) | 76, 245, 525, 538, 568, 589 |
| BmiI | GGNNCC | 6 cut(s) | 30, 78, 195, 246, 527, 609 |
| BmrFI | CCNGG | 4 cut(s) | 26, 201, 242, 264 |
| BmsI | GCATC | 2 cut(s) | 106, 464 |
| BpmI | CTGGAG | 2 cut(s) | 78, 183 |
| BpuEI | CTTGAG | 1 cut(s) | 267 |
| BpuMI | CCSGG | 1 cut(s) | 264 |
| BsaJI | CCNNGG | 6 cut(s) | 24, 200, 241, 393, 469, 564 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 38, 98, 535 |
| Bse1I | ACTGG | 2 cut(s) | 95, 600 |
| BseBI | CCWGG | 3 cut(s) | 26, 201, 242 |
| BseDI | CCNNGG | 6 cut(s) | 24, 200, 241, 393, 469, 564 |
| BseGI | GGATG | 2 cut(s) | 217, 455 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 38, 98, 535 |
| BseMII | CTCAG | 1 cut(s) | 491 |
| BseNI | ACTGG | 2 cut(s) | 95, 600 |
| BseRI | GAGGAG | 3 cut(s) | 132, 186, 620 |
| BseXI | GCAGC | 5 cut(s) | 94, 97, 244, 281, 385 |
| BsgI | GTGCAG | 2 cut(s) | 288, 384 |
| Bsh1236I | CGCG | 8 cut(s) | 180, 209, 309, 371, 373, 395, 432, 462 |
| Bsh1285I | CGRYCG | 1 cut(s) | 393 |
| BshFI | GGCC | 4 cut(s) | 102, 435, 569, 590 |
| BsiEI | CGRYCG | 1 cut(s) | 393 |
| BsiHKAI | GWGCWC | 2 cut(s) | 283, 354 |
| BsiSI | CCGG | 1 cut(s) | 263 |
| BslFI | GGGAC | 2 cut(s) | 62, 122 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 38, 98, 535 |
| BsmFI | GGGAC | 2 cut(s) | 62, 122 |
| BsnI | GGCC | 4 cut(s) | 102, 435, 569, 590 |
| Bsp1286I | GDGCHC | 3 cut(s) | 283, 354, 612 |
| Bsp143I | GATC | 5 cut(s) | 7, 81, 475, 496, 504 |
| BspANI | GGCC | 4 cut(s) | 102, 435, 569, 590 |
| BspCNI | CTCAG | 1 cut(s) | 492 |
| BspFNI | CGCG | 8 cut(s) | 180, 209, 309, 371, 373, 395, 432, 462 |
| BspLI | GGNNCC | 6 cut(s) | 30, 78, 195, 246, 527, 609 |
| BspMAI | CTGCAG | 1 cut(s) | 234 |
| BspMI | ACCTGC | 3 cut(s) | 25, 29, 403 |
| BspPI | GGATC | 3 cut(s) | 15, 504, 512 |
| BsrI | ACTGG | 2 cut(s) | 95, 600 |
| BssECI | CCNNGG | 6 cut(s) | 24, 200, 241, 393, 469, 564 |
| BssMI | GATC | 5 cut(s) | 7, 81, 475, 496, 504 |
| BssT1I | CCWWGG | 2 cut(s) | 469, 564 |
| Bst2UI | CCWGG | 3 cut(s) | 26, 201, 242 |
| Bst4CI | ACNGT | 1 cut(s) | 583 |
| BstDEI | CTNAG | 1 cut(s) | 500 |
| BstDSI | CCRYGG | 1 cut(s) | 393 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 96 |
| BstF5I | GGATG | 2 cut(s) | 217, 455 |
| BstFNI | CGCG | 8 cut(s) | 180, 209, 309, 371, 373, 395, 432, 462 |
| BstH2I | RGCGCY | 1 cut(s) | 485 |
| BstHHI | GCGC | 3 cut(s) | 373, 484, 601 |
| BstKTI | GATC | 5 cut(s) | 10, 84, 478, 499, 507 |
| BstMBI | GATC | 5 cut(s) | 7, 81, 475, 496, 504 |
| BstMCI | CGRYCG | 1 cut(s) | 393 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 116, 215, 278, 446, 596, 607 |
| BstNI | CCWGG | 3 cut(s) | 26, 201, 242 |
| BstNSI | RCATGY | 1 cut(s) | 163 |
| BstSCI | CCNGG | 4 cut(s) | 24, 199, 240, 262 |
| BstSFI | CTRYAG | 1 cut(s) | 230 |
| BstUI | CGCG | 8 cut(s) | 180, 209, 309, 371, 373, 395, 432, 462 |
| BstV1I | GCAGC | 5 cut(s) | 94, 97, 244, 281, 385 |
| BstX2I | RGATCY | 2 cut(s) | 496, 504 |
| BstYI | RGATCY | 2 cut(s) | 496, 504 |
| BsuRI | GGCC | 4 cut(s) | 102, 435, 569, 590 |
| BtgI | CCRYGG | 1 cut(s) | 393 |
| BtgZI | GCGATG | 1 cut(s) | 112 |
| BtsCI | GGATG | 2 cut(s) | 217, 455 |
| BveI | ACCTGC | 3 cut(s) | 25, 29, 403 |
| CaiI | CAGNNNCTG | 1 cut(s) | 44 |
| CfoI | GCGC | 3 cut(s) | 373, 484, 601 |
| Cfr13I | GGNCC | 6 cut(s) | 76, 245, 525, 538, 568, 589 |
| Cfr42I | CCGCGG | 1 cut(s) | 396 |
| Csp6I | GTAC | 4 cut(s) | 59, 182, 299, 315 |
| CviAII | CATG | 1 cut(s) | 160 |
| CviQI | GTAC | 4 cut(s) | 59, 182, 299, 315 |
| DdeI | CTNAG | 1 cut(s) | 500 |
| DpnI | GATC | 5 cut(s) | 9, 83, 477, 498, 506 |
| DpnII | GATC | 5 cut(s) | 7, 81, 475, 496, 504 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 243 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 243 |
| EciI | GGCGGA | 1 cut(s) | 20 |
| Ecl136II | GAGCTC | 2 cut(s) | 281, 352 |
| Eco130I | CCWWGG | 2 cut(s) | 469, 564 |
| Eco147I | AGGCCT | 1 cut(s) | 102 |
| Eco24I | GRGCYC | 3 cut(s) | 283, 354, 612 |
| Eco47I | GGWCC | 4 cut(s) | 76, 245, 525, 538 |
| Eco47III | AGCGCT | 1 cut(s) | 483 |
| Eco53kI | GAGCTC | 2 cut(s) | 281, 352 |
| Eco57I | CTGAAG | 1 cut(s) | 153 |
| EcoICRI | GAGCTC | 2 cut(s) | 281, 352 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 96 |
| EcoO109I | RGGNCCY | 3 cut(s) | 76, 245, 538 |
| EcoRII | CCWGG | 3 cut(s) | 24, 199, 240 |
| EcoT14I | CCWWGG | 2 cut(s) | 469, 564 |
| EcoT38I | GRGCYC | 3 cut(s) | 283, 354, 612 |
| ErhI | CCWWGG | 2 cut(s) | 469, 564 |
| FaeI | CATG | 1 cut(s) | 163 |
| FaiI | YATR | 3 cut(s) | 161, 346, 348 |
| FaqI | GGGAC | 2 cut(s) | 62, 122 |
| FatI | CATG | 1 cut(s) | 159 |
| FauI | CCCGC | 2 cut(s) | 376, 467 |
| FauNDI | CATATG | 1 cut(s) | 346 |
| Fnu4HI | GCNGC | 8 cut(s) | 108, 111, 233, 270, 307, 396, 399, 433 |
| FokI | GGATG | 2 cut(s) | 224, 442 |
| FriOI | GRGCYC | 3 cut(s) | 283, 354, 612 |
| Fsp4HI | GCNGC | 8 cut(s) | 108, 111, 233, 270, 307, 396, 399, 433 |
| FspBI | CTAG | 2 cut(s) | 185, 470 |
| GlaI | GCGC | 3 cut(s) | 372, 483, 600 |
| GluI | GCNGC | 8 cut(s) | 108, 111, 233, 270, 307, 396, 399, 433 |
| GsuI | CTGGAG | 2 cut(s) | 78, 183 |
| HaeII | RGCGCY | 1 cut(s) | 485 |
| HaeIII | GGCC | 4 cut(s) | 102, 435, 569, 590 |
| HapII | CCGG | 1 cut(s) | 263 |
| HhaI | GCGC | 3 cut(s) | 373, 484, 601 |
| Hin1II | CATG | 1 cut(s) | 163 |
| Hin6I | GCGC | 3 cut(s) | 371, 482, 599 |
| HinP1I | GCGC | 3 cut(s) | 371, 482, 599 |
| HinfI | GANTC | 2 cut(s) | 237, 464 |
| HpaII | CCGG | 1 cut(s) | 263 |
| HphI | GGTGA | 1 cut(s) | 485 |
| Hpy188I | TCNGA | 3 cut(s) | 294, 501, 525 |
| Hpy188III | TCNNGA | 6 cut(s) | 11, 32, 79, 248, 284, 536 |
| HpyAV | CCTTC | 2 cut(s) | 374, 530 |
| HpyCH4III | ACNGT | 1 cut(s) | 583 |
| HpyCH4IV | ACGT | 1 cut(s) | 313 |
| HpyCH4V | TGCA | 6 cut(s) | 56, 232, 269, 358, 401, 440 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 116, 215, 278, 446, 596, 607 |
| HpyF3I | CTNAG | 1 cut(s) | 500 |
| HpySE526I | ACGT | 1 cut(s) | 313 |
| Hsp92II | CATG | 1 cut(s) | 163 |
| HspAI | GCGC | 3 cut(s) | 371, 482, 599 |
| KspI | CCGCGG | 1 cut(s) | 396 |
| Kzo9I | GATC | 5 cut(s) | 7, 81, 475, 496, 504 |
| LmnI | GCTCC | 3 cut(s) | 199, 357, 607 |
| Lsp1109I | GCAGC | 5 cut(s) | 94, 97, 244, 281, 385 |
| LweI | GCATC | 2 cut(s) | 106, 464 |
| MaeI | CTAG | 2 cut(s) | 185, 470 |
| MaeII | ACGT | 1 cut(s) | 313 |
| MaeIII | GTNAC | 2 cut(s) | 577, 583 |
| MalI | GATC | 5 cut(s) | 9, 83, 477, 498, 506 |
| MboI | GATC | 5 cut(s) | 7, 81, 475, 496, 504 |
| MflI | RGATCY | 2 cut(s) | 496, 504 |
| MhlI | GDGCHC | 3 cut(s) | 283, 354, 612 |
| MluCI | AATT | 2 cut(s) | 332, 572 |
| MlyI | GAGTC | 2 cut(s) | 231, 458 |
| MseI | TTAA | 1 cut(s) | 378 |
| MspA1I | CMGCKG | 1 cut(s) | 395 |
| MspI | CCGG | 1 cut(s) | 263 |
| MspR9I | CCNGG | 4 cut(s) | 26, 201, 242, 264 |
| MvaI | CCWGG | 3 cut(s) | 26, 201, 242 |
| MvnI | CGCG | 8 cut(s) | 180, 209, 309, 371, 373, 395, 432, 462 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 116, 215, 278, 446, 596, 607 |
| NciI | CCSGG | 1 cut(s) | 264 |
| NdeI | CATATG | 1 cut(s) | 346 |
| NdeII | GATC | 5 cut(s) | 7, 81, 475, 496, 504 |
| NlaIII | CATG | 1 cut(s) | 163 |
| NlaIV | GGNNCC | 6 cut(s) | 30, 78, 195, 246, 527, 609 |
| NmuCI | GTSAC | 2 cut(s) | 577, 583 |
| NspI | RCATGY | 1 cut(s) | 163 |
| PaqCI | CACCTGC | 1 cut(s) | 29 |
| PceI | AGGCCT | 1 cut(s) | 102 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 310, 484 |
| PkrI | GCNGC | 8 cut(s) | 109, 112, 234, 271, 308, 397, 400, 434 |
| PleI | GAGTC | 2 cut(s) | 231, 458 |
| PpsI | GAGTC | 2 cut(s) | 231, 458 |
| PpuMI | RGGWCCY | 3 cut(s) | 76, 245, 538 |
| Psp124BI | GAGCTC | 2 cut(s) | 283, 354 |
| Psp5II | RGGWCCY | 3 cut(s) | 76, 245, 538 |
| Psp6I | CCWGG | 3 cut(s) | 24, 199, 240 |
| PspGI | CCWGG | 3 cut(s) | 24, 199, 240 |
| PspN4I | GGNNCC | 6 cut(s) | 30, 78, 195, 246, 527, 609 |
| PspPI | GGNCC | 6 cut(s) | 76, 245, 525, 538, 568, 589 |
| PspPPI | RGGWCCY | 3 cut(s) | 76, 245, 538 |
| PstI | CTGCAG | 1 cut(s) | 234 |
| PstNI | CAGNNNCTG | 1 cut(s) | 44 |
| PsuI | RGATCY | 2 cut(s) | 496, 504 |
| RsaI | GTAC | 4 cut(s) | 60, 183, 300, 316 |
| RsaNI | GTAC | 4 cut(s) | 59, 182, 299, 315 |
| SacI | GAGCTC | 2 cut(s) | 283, 354 |
| SacII | CCGCGG | 1 cut(s) | 396 |
| SaqAI | TTAA | 1 cut(s) | 378 |
| SatI | GCNGC | 8 cut(s) | 108, 111, 233, 270, 307, 396, 399, 433 |
| Sau3AI | GATC | 5 cut(s) | 7, 81, 475, 496, 504 |
| Sau96I | GGNCC | 6 cut(s) | 76, 245, 525, 538, 568, 589 |
| SchI | GAGTC | 2 cut(s) | 231, 458 |
| ScrFI | CCNGG | 4 cut(s) | 26, 201, 242, 264 |
| SduI | GDGCHC | 3 cut(s) | 283, 354, 612 |
| SfaNI | GCATC | 2 cut(s) | 106, 464 |
| SfcI | CTRYAG | 1 cut(s) | 230 |
| Sfr303I | CCGCGG | 1 cut(s) | 396 |
| SgrBI | CCGCGG | 1 cut(s) | 396 |
| SinI | GGWCC | 4 cut(s) | 76, 245, 525, 538 |
| SmlI | CTYRAG | 1 cut(s) | 282 |
| SmoI | CTYRAG | 1 cut(s) | 282 |
| Sse9I | AATT | 2 cut(s) | 332, 572 |
| SseBI | AGGCCT | 1 cut(s) | 102 |
| SspMI | CTAG | 2 cut(s) | 185, 470 |
| SstI | GAGCTC | 2 cut(s) | 283, 354 |
| StuI | AGGCCT | 1 cut(s) | 102 |
| StyD4I | CCNGG | 4 cut(s) | 24, 199, 240, 262 |
| StyI | CCWWGG | 2 cut(s) | 469, 564 |
| TaaI | ACNGT | 1 cut(s) | 583 |
| TaiI | ACGT | 1 cut(s) | 316 |
| TaqI | TCGA | 4 cut(s) | 87, 478, 543, 558 |
| TasI | AATT | 2 cut(s) | 332, 572 |
| TauI | GCSGC | 3 cut(s) | 309, 398, 435 |
| Tru1I | TTAA | 1 cut(s) | 378 |
| Tru9I | TTAA | 1 cut(s) | 378 |
| TseFI | GTSAC | 2 cut(s) | 577, 583 |
| TseI | GCWGC | 5 cut(s) | 107, 110, 232, 269, 398 |
| Tsp45I | GTSAC | 2 cut(s) | 577, 583 |
| TspDTI | ATGAA | 2 cut(s) | 228, 345 |
| VpaK11BI | GGWCC | 4 cut(s) | 76, 245, 525, 538 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 96 |
| XapI | RAATTY | 1 cut(s) | 332 |
| XceI | RCATGY | 1 cut(s) | 163 |
| XmaJI | CCTAGG | 1 cut(s) | 469 |
| XspI | CTAG | 2 cut(s) | 185, 470 |