No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 696 |
| AbsI | CCTCGAGG | 1 cut(s) | 718 |
| Acc16I | TGCGCA | 1 cut(s) | 423 |
| Acc36I | ACCTGC | 1 cut(s) | 427 |
| AccB1I | GGYRCC | 1 cut(s) | 880 |
| AccBSI | CCGCTC | 1 cut(s) | 493 |
| AccI | GTMKAC | 1 cut(s) | 116 |
| AccII | CGCG | 5 cut(s) | 105, 260, 279, 432, 477 |
| AccIII | TCCGGA | 1 cut(s) | 512 |
| AclWI | GGATC | 7 cut(s) | 12, 233, 246, 650, 699, 712, 731 |
| AcoI | YGGCCR | 2 cut(s) | 72, 662 |
| AcsI | RAATTY | 1 cut(s) | 24 |
| AfaI | GTAC | 3 cut(s) | 156, 624, 846 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 235, 371, 436, 512, 648, 660, 877 |
| AflIII | ACRYGT | 1 cut(s) | 258 |
| AgeI | ACCGGT | 1 cut(s) | 691 |
| AgsI | TTSAA | 3 cut(s) | 23, 218, 851 |
| AjnI | CCWGG | 1 cut(s) | 816 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 210, 242 |
| AluBI | AGCT | 4 cut(s) | 82, 304, 331, 427 |
| AluI | AGCT | 4 cut(s) | 82, 304, 331, 427 |
| Alw21I | GWGCWC | 3 cut(s) | 333, 568, 716 |
| Alw26I | GTCTC | 1 cut(s) | 897 |
| AlwI | GGATC | 7 cut(s) | 12, 233, 246, 650, 699, 712, 731 |
| Ama87I | CYCGRG | 3 cut(s) | 647, 718, 872 |
| Aor13HI | TCCGGA | 1 cut(s) | 512 |
| AoxI | GGCC | 2 cut(s) | 72, 662 |
| ApeKI | GCWGC | 2 cut(s) | 424, 611 |
| ApoI | RAATTY | 1 cut(s) | 24 |
| AsiGI | ACCGGT | 1 cut(s) | 691 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 890 |
| AspLEI | GCGC | 2 cut(s) | 281, 424 |
| AspS9I | GGNCC | 1 cut(s) | 651 |
| AsuC2I | CCSGG | 3 cut(s) | 162, 648, 649 |
| AsuHPI | GGTGA | 1 cut(s) | 812 |
| AvaI | CYCGRG | 3 cut(s) | 647, 718, 872 |
| AvaII | GGWCC | 1 cut(s) | 651 |
| BamHI | GGATCC | 3 cut(s) | 4, 238, 704 |
| BanI | GGYRCC | 1 cut(s) | 880 |
| BanII | GRGCYC | 3 cut(s) | 333, 447, 483 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 493, 525 |
| BauI | CACGAG | 2 cut(s) | 401, 709 |
| BbsI | GAAGAC | 1 cut(s) | 675 |
| Bbv12I | GWGCWC | 3 cut(s) | 333, 568, 716 |
| BbvI | GCAGC | 2 cut(s) | 436, 598 |
| BccI | CCATC | 1 cut(s) | 743 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 26, 60 |
| BciT130I | CCWGG | 1 cut(s) | 818 |
| BciVI | GTATCC | 1 cut(s) | 372 |
| BcnI | CCSGG | 3 cut(s) | 162, 648, 649 |
| BcoDI | GTCTC | 1 cut(s) | 897 |
| BfaI | CTAG | 2 cut(s) | 620, 743 |
| BfmI | CTRYAG | 1 cut(s) | 247 |
| BfuAI | ACCTGC | 1 cut(s) | 427 |
| BfuI | GTATCC | 1 cut(s) | 372 |
| BisI | GCNGC | 4 cut(s) | 277, 309, 425, 612 |
| BlsI | GCNGC | 4 cut(s) | 278, 310, 426, 613 |
| BmcAI | AGTACT | 1 cut(s) | 846 |
| Bme1390I | CCNGG | 4 cut(s) | 162, 648, 649, 818 |
| Bme18I | GGWCC | 1 cut(s) | 651 |
| BmeT110I | CYCGRG | 3 cut(s) | 647, 718, 872 |
| BmgT120I | GGNCC | 1 cut(s) | 651 |
| BmiI | GGNNCC | 7 cut(s) | 6, 227, 240, 652, 706, 822, 882 |
| BmrFI | CCNGG | 4 cut(s) | 162, 648, 649, 818 |
| BmsI | GCATC | 2 cut(s) | 160, 520 |
| BpiI | GAAGAC | 1 cut(s) | 675 |
| BpuEI | CTTGAG | 3 cut(s) | 376, 442, 471 |
| BpuMI | CCSGG | 3 cut(s) | 162, 648, 649 |
| BsaAI | YACGTR | 1 cut(s) | 626 |
| BsaJI | CCNNGG | 3 cut(s) | 646, 647, 659 |
| BsaWI | WCCGGW | 2 cut(s) | 512, 691 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 623, 653 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 235, 371, 436, 512, 648, 660, 877 |
| Bse118I | RCCGGY | 4 cut(s) | 70, 691, 773, 877 |
| Bse3DI | GCAATG | 1 cut(s) | 606 |
| BseAI | TCCGGA | 1 cut(s) | 512 |
| BseBI | CCWGG | 1 cut(s) | 818 |
| BseDI | CCNNGG | 3 cut(s) | 646, 647, 659 |
| BseGI | GGATG | 2 cut(s) | 119, 412 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 235, 371, 436, 512, 648, 660, 877 |
| BseMI | GCAATG | 1 cut(s) | 606 |
| BseRI | GAGGAG | 1 cut(s) | 572 |
| BseX3I | CGGCCG | 1 cut(s) | 72 |
| BseXI | GCAGC | 2 cut(s) | 436, 598 |
| Bsh1236I | CGCG | 5 cut(s) | 105, 260, 279, 432, 477 |
| Bsh1285I | CGRYCG | 3 cut(s) | 75, 637, 692 |
| BshFI | GGCC | 2 cut(s) | 74, 664 |
| BshNI | GGYRCC | 1 cut(s) | 880 |
| BshTI | ACCGGT | 1 cut(s) | 691 |
| BsiEI | CGRYCG | 3 cut(s) | 75, 637, 692 |
| BsiHKAI | GWGCWC | 3 cut(s) | 333, 568, 716 |
| BsiHKCI | CYCGRG | 3 cut(s) | 647, 718, 872 |
| BsiSI | CCGG | 8 cut(s) | 71, 162, 513, 648, 665, 692, 774, 878 |
| BslFI | GGGAC | 2 cut(s) | 664, 821 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 235, 371, 436, 512, 648, 660, 877 |
| BsmAI | GTCTC | 1 cut(s) | 897 |
| BsmFI | GGGAC | 2 cut(s) | 664, 821 |
| BsmI | GAATGC | 1 cut(s) | 471 |
| BsnI | GGCC | 2 cut(s) | 74, 664 |
| BsoBI | CYCGRG | 3 cut(s) | 647, 718, 872 |
| Bsp1286I | GDGCHC | 5 cut(s) | 333, 447, 483, 568, 716 |
| Bsp13I | TCCGGA | 1 cut(s) | 512 |
| Bsp143I | GATC | 7 cut(s) | 4, 238, 253, 655, 685, 704, 723 |
| Bsp68I | TCGCGA | 1 cut(s) | 477 |
| BspANI | GGCC | 2 cut(s) | 74, 664 |
| BspEI | TCCGGA | 1 cut(s) | 512 |
| BspFNI | CGCG | 5 cut(s) | 105, 260, 279, 432, 477 |
| BspLI | GGNNCC | 7 cut(s) | 6, 227, 240, 652, 706, 822, 882 |
| BspMI | ACCTGC | 1 cut(s) | 427 |
| BspPI | GGATC | 7 cut(s) | 12, 233, 246, 650, 699, 712, 731 |
| BspQI | GCTCTTC | 1 cut(s) | 338 |
| BspT107I | GGYRCC | 1 cut(s) | 880 |
| BsrBI | CCGCTC | 1 cut(s) | 493 |
| BsrDI | GCAATG | 1 cut(s) | 606 |
| BsrFI | RCCGGY | 4 cut(s) | 70, 691, 773, 877 |
| BssAI | RCCGGY | 4 cut(s) | 70, 691, 773, 877 |
| BssECI | CCNNGG | 3 cut(s) | 646, 647, 659 |
| BssMI | GATC | 7 cut(s) | 4, 238, 253, 655, 685, 704, 723 |
| BssNAI | GTATAC | 1 cut(s) | 117 |
| BssSI | CACGAG | 2 cut(s) | 401, 709 |
| BssT1I | CCWWGG | 1 cut(s) | 659 |
| Bst1107I | GTATAC | 1 cut(s) | 117 |
| Bst2BI | CACGAG | 2 cut(s) | 401, 709 |
| Bst2UI | CCWGG | 1 cut(s) | 818 |
| Bst4CI | ACNGT | 1 cut(s) | 635 |
| Bst6I | CTCTTC | 3 cut(s) | 338, 350, 759 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 419 |
| BstBAI | YACGTR | 1 cut(s) | 626 |
| BstC8I | GCNNGC | 7 cut(s) | 262, 306, 390, 394, 443, 479, 775 |
| BstDEI | CTNAG | 2 cut(s) | 191, 381 |
| BstF5I | GGATG | 2 cut(s) | 119, 412 |
| BstFNI | CGCG | 5 cut(s) | 105, 260, 279, 432, 477 |
| BstHHI | GCGC | 2 cut(s) | 281, 424 |
| BstKTI | GATC | 7 cut(s) | 7, 241, 256, 658, 688, 707, 726 |
| BstMAI | GTCTC | 1 cut(s) | 897 |
| BstMBI | GATC | 7 cut(s) | 4, 238, 253, 655, 685, 704, 723 |
| BstMCI | CGRYCG | 3 cut(s) | 75, 637, 692 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 419, 438, 447, 468, 530 |
| BstNI | CCWGG | 1 cut(s) | 818 |
| BstSCI | CCNGG | 4 cut(s) | 160, 646, 647, 816 |
| BstSFI | CTRYAG | 1 cut(s) | 247 |
| BstSNI | TACGTA | 1 cut(s) | 626 |
| BstUI | CGCG | 5 cut(s) | 105, 260, 279, 432, 477 |
| BstV1I | GCAGC | 2 cut(s) | 436, 598 |
| BstV2I | GAAGAC | 1 cut(s) | 675 |
| BstX2I | RGATCY | 3 cut(s) | 4, 238, 704 |
| BstYI | RGATCY | 3 cut(s) | 4, 238, 704 |
| BstZ17I | GTATAC | 1 cut(s) | 117 |
| BstZI | CGGCCG | 1 cut(s) | 72 |
| BsuI | GTATCC | 1 cut(s) | 372 |
| BsuRI | GGCC | 2 cut(s) | 74, 664 |
| BtsCI | GGATG | 2 cut(s) | 119, 412 |
| BtuMI | TCGCGA | 1 cut(s) | 477 |
| BveI | ACCTGC | 1 cut(s) | 427 |
| Cac8I | GCNNGC | 7 cut(s) | 262, 306, 390, 394, 443, 479, 775 |
| CfoI | GCGC | 2 cut(s) | 281, 424 |
| Cfr10I | RCCGGY | 4 cut(s) | 70, 691, 773, 877 |
| Cfr13I | GGNCC | 1 cut(s) | 651 |
| Cfr9I | CCCGGG | 1 cut(s) | 647 |
| Csp6I | GTAC | 3 cut(s) | 155, 623, 845 |
| CspAI | ACCGGT | 1 cut(s) | 691 |
| CviAII | CATG | 2 cut(s) | 221, 833 |
| CviQI | GTAC | 3 cut(s) | 155, 623, 845 |
| DdeI | CTNAG | 2 cut(s) | 191, 381 |
| DpnI | GATC | 7 cut(s) | 6, 240, 255, 657, 687, 706, 725 |
| DpnII | GATC | 7 cut(s) | 4, 238, 253, 655, 685, 704, 723 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 696 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 696 |
| EaeI | YGGCCR | 2 cut(s) | 72, 662 |
| EagI | CGGCCG | 1 cut(s) | 72 |
| Eam1104I | CTCTTC | 3 cut(s) | 338, 350, 759 |
| EarI | CTCTTC | 3 cut(s) | 338, 350, 759 |
| Ecl136II | GAGCTC | 1 cut(s) | 331 |
| EclXI | CGGCCG | 1 cut(s) | 72 |
| Eco105I | TACGTA | 1 cut(s) | 626 |
| Eco130I | CCWWGG | 1 cut(s) | 659 |
| Eco24I | GRGCYC | 3 cut(s) | 333, 447, 483 |
| Eco32I | GATATC | 1 cut(s) | 298 |
| Eco47I | GGWCC | 1 cut(s) | 651 |
| Eco52I | CGGCCG | 1 cut(s) | 72 |
| Eco53kI | GAGCTC | 1 cut(s) | 331 |
| Eco88I | CYCGRG | 3 cut(s) | 647, 718, 872 |
| EcoICRI | GAGCTC | 1 cut(s) | 331 |
| EcoRII | CCWGG | 1 cut(s) | 816 |
| EcoRV | GATATC | 1 cut(s) | 298 |
| EcoT14I | CCWWGG | 1 cut(s) | 659 |
| EcoT38I | GRGCYC | 3 cut(s) | 333, 447, 483 |
| ErhI | CCWWGG | 1 cut(s) | 659 |
| FaeI | CATG | 2 cut(s) | 224, 836 |
| FaiI | YATR | 7 cut(s) | 100, 117, 148, 176, 222, 503, 834 |
| FaqI | GGGAC | 2 cut(s) | 664, 821 |
| FatI | CATG | 2 cut(s) | 220, 832 |
| FauI | CCCGC | 5 cut(s) | 42, 213, 455, 793, 847 |
| FblI | GTMKAC | 1 cut(s) | 116 |
| Fnu4HI | GCNGC | 4 cut(s) | 277, 309, 425, 612 |
| FokI | GGATG | 2 cut(s) | 106, 419 |
| FriOI | GRGCYC | 3 cut(s) | 333, 447, 483 |
| Fsp4HI | GCNGC | 4 cut(s) | 277, 309, 425, 612 |
| FspBI | CTAG | 2 cut(s) | 620, 743 |
| FspI | TGCGCA | 1 cut(s) | 423 |
| GlaI | GCGC | 2 cut(s) | 280, 423 |
| GluI | GCNGC | 4 cut(s) | 277, 309, 425, 612 |
| HaeIII | GGCC | 2 cut(s) | 74, 664 |
| HapII | CCGG | 8 cut(s) | 71, 162, 513, 648, 665, 692, 774, 878 |
| HhaI | GCGC | 2 cut(s) | 281, 424 |
| Hin1II | CATG | 2 cut(s) | 224, 836 |
| Hin6I | GCGC | 2 cut(s) | 279, 422 |
| HinP1I | GCGC | 2 cut(s) | 279, 422 |
| HincII | GTYRAC | 1 cut(s) | 814 |
| HindII | GTYRAC | 1 cut(s) | 814 |
| HinfI | GANTC | 4 cut(s) | 201, 465, 579, 905 |
| HpaII | CCGG | 8 cut(s) | 71, 162, 513, 648, 665, 692, 774, 878 |
| HphI | GGTGA | 1 cut(s) | 812 |
| Hpy166II | GTNNAC | 3 cut(s) | 117, 155, 814 |
| Hpy188I | TCNGA | 3 cut(s) | 136, 295, 643 |
| Hpy188III | TCNNGA | 6 cut(s) | 47, 242, 476, 488, 513, 909 |
| Hpy8I | GTNNAC | 3 cut(s) | 117, 155, 814 |
| Hpy99I | CGWCG | 3 cut(s) | 48, 80, 759 |
| HpyAV | CCTTC | 4 cut(s) | 239, 365, 519, 854 |
| HpyCH4III | ACNGT | 1 cut(s) | 635 |
| HpyCH4IV | ACGT | 1 cut(s) | 625 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 419, 438, 447, 468, 530 |
| HpyF3I | CTNAG | 2 cut(s) | 191, 381 |
| HpySE526I | ACGT | 1 cut(s) | 625 |
| Hsp92II | CATG | 2 cut(s) | 224, 836 |
| HspAI | GCGC | 2 cut(s) | 279, 422 |
| Kpn2I | TCCGGA | 1 cut(s) | 512 |
| KroI | GCCGGC | 1 cut(s) | 773 |
| KroNI | GCCGGC | 1 cut(s) | 775 |
| Kzo9I | GATC | 7 cut(s) | 4, 238, 253, 655, 685, 704, 723 |
| LguI | GCTCTTC | 1 cut(s) | 338 |
| LmnI | GCTCC | 4 cut(s) | 432, 563, 644, 740 |
| Lsp1109I | GCAGC | 2 cut(s) | 436, 598 |
| LweI | GCATC | 2 cut(s) | 160, 520 |
| MaeI | CTAG | 2 cut(s) | 620, 743 |
| MaeII | ACGT | 1 cut(s) | 625 |
| MaeIII | GTNAC | 2 cut(s) | 47, 698 |
| MalI | GATC | 7 cut(s) | 6, 240, 255, 657, 687, 706, 725 |
| MbiI | CCGCTC | 1 cut(s) | 493 |
| MboI | GATC | 7 cut(s) | 4, 238, 253, 655, 685, 704, 723 |
| MboII | GAAGA | 5 cut(s) | 210, 325, 337, 680, 776 |
| MflI | RGATCY | 3 cut(s) | 4, 238, 704 |
| MhlI | GDGCHC | 5 cut(s) | 333, 447, 483, 568, 716 |
| MluCI | AATT | 1 cut(s) | 24 |
| MluI | ACGCGT | 1 cut(s) | 258 |
| MlyI | GAGTC | 2 cut(s) | 573, 899 |
| MmeI | TCCRAC | 1 cut(s) | 666 |
| MroI | TCCGGA | 1 cut(s) | 512 |
| MroNI | GCCGGC | 1 cut(s) | 773 |
| MroXI | GAANNNNTTC | 1 cut(s) | 890 |
| MslI | CAYNNNNRTG | 1 cut(s) | 405 |
| MspI | CCGG | 8 cut(s) | 71, 162, 513, 648, 665, 692, 774, 878 |
| MspR9I | CCNGG | 4 cut(s) | 162, 648, 649, 818 |
| Mva1269I | GAATGC | 1 cut(s) | 471 |
| MvaI | CCWGG | 1 cut(s) | 818 |
| MvnI | CGCG | 5 cut(s) | 105, 260, 279, 432, 477 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 419, 438, 447, 468, 530 |
| NaeI | GCCGGC | 1 cut(s) | 775 |
| NciI | CCSGG | 3 cut(s) | 162, 648, 649 |
| NdeII | GATC | 7 cut(s) | 4, 238, 253, 655, 685, 704, 723 |
| NgoMIV | GCCGGC | 1 cut(s) | 773 |
| NlaIII | CATG | 2 cut(s) | 224, 836 |
| NlaIV | GGNNCC | 7 cut(s) | 6, 227, 240, 652, 706, 822, 882 |
| NmeAIII | GCCGAG | 1 cut(s) | 814 |
| NmuCI | GTSAC | 2 cut(s) | 47, 698 |
| NruI | TCGCGA | 1 cut(s) | 477 |
| NsbI | TGCGCA | 1 cut(s) | 423 |
| PaeR7I | CTCGAG | 1 cut(s) | 718 |
| PciSI | GCTCTTC | 1 cut(s) | 338 |
| PcsI | WCGNNNNNNNCGW | 5 cut(s) | 474, 591, 639, 694, 716 |
| PctI | GAATGC | 1 cut(s) | 471 |
| PdiI | GCCGGC | 1 cut(s) | 775 |
| PdmI | GAANNNNTTC | 1 cut(s) | 890 |
| PfeI | GAWTC | 2 cut(s) | 201, 465 |
| PfoI | TCCNGGA | 1 cut(s) | 160 |
| PinAI | ACCGGT | 1 cut(s) | 691 |
| PkrI | GCNGC | 4 cut(s) | 278, 310, 426, 613 |
| PleI | GAGTC | 2 cut(s) | 573, 899 |
| PpsI | GAGTC | 2 cut(s) | 573, 899 |
| Ppu21I | YACGTR | 1 cut(s) | 626 |
| Psp124BI | GAGCTC | 1 cut(s) | 333 |
| Psp6I | CCWGG | 1 cut(s) | 816 |
| PspGI | CCWGG | 1 cut(s) | 816 |
| PspN4I | GGNNCC | 7 cut(s) | 6, 227, 240, 652, 706, 822, 882 |
| PspPI | GGNCC | 1 cut(s) | 651 |
| PspXI | VCTCGAGB | 1 cut(s) | 718 |
| PsuI | RGATCY | 3 cut(s) | 4, 238, 704 |
| RruI | TCGCGA | 1 cut(s) | 477 |
| RsaI | GTAC | 3 cut(s) | 156, 624, 846 |
| RsaNI | GTAC | 3 cut(s) | 155, 623, 845 |
| RseI | CAYNNNNRTG | 1 cut(s) | 405 |
| SacI | GAGCTC | 1 cut(s) | 333 |
| SapI | GCTCTTC | 1 cut(s) | 338 |
| SatI | GCNGC | 4 cut(s) | 277, 309, 425, 612 |
| Sau3AI | GATC | 7 cut(s) | 4, 238, 253, 655, 685, 704, 723 |
| Sau96I | GGNCC | 1 cut(s) | 651 |
| ScaI | AGTACT | 1 cut(s) | 846 |
| SchI | GAGTC | 2 cut(s) | 573, 899 |
| ScrFI | CCNGG | 4 cut(s) | 162, 648, 649, 818 |
| SduI | GDGCHC | 5 cut(s) | 333, 447, 483, 568, 716 |
| SfaNI | GCATC | 2 cut(s) | 160, 520 |
| SfcI | CTRYAG | 1 cut(s) | 247 |
| Sfr274I | CTCGAG | 1 cut(s) | 718 |
| SinI | GGWCC | 1 cut(s) | 651 |
| SlaI | CTCGAG | 1 cut(s) | 718 |
| SmaI | CCCGGG | 1 cut(s) | 649 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 405 |
| SmlI | CTYRAG | 4 cut(s) | 355, 457, 486, 718 |
| SmoI | CTYRAG | 4 cut(s) | 355, 457, 486, 718 |
| SnaBI | TACGTA | 1 cut(s) | 626 |
| Sse9I | AATT | 1 cut(s) | 24 |
| SspMI | CTAG | 2 cut(s) | 620, 743 |
| SstI | GAGCTC | 1 cut(s) | 333 |
| StyD4I | CCNGG | 4 cut(s) | 160, 646, 647, 816 |
| StyI | CCWWGG | 1 cut(s) | 659 |
| TaaI | ACNGT | 1 cut(s) | 635 |
| TaiI | ACGT | 1 cut(s) | 628 |
| TaqI | TCGA | 6 cut(s) | 10, 321, 585, 688, 719, 726 |
| TaqII | GACCGA | 1 cut(s) | 668 |
| TasI | AATT | 1 cut(s) | 24 |
| TatI | WGTACW | 1 cut(s) | 844 |
| TauI | GCSGC | 2 cut(s) | 279, 311 |
| TfiI | GAWTC | 2 cut(s) | 201, 465 |
| TseFI | GTSAC | 2 cut(s) | 47, 698 |
| TseI | GCWGC | 2 cut(s) | 424, 611 |
| Tsp45I | GTSAC | 2 cut(s) | 47, 698 |
| TspDTI | ATGAA | 2 cut(s) | 330, 423 |
| TspGWI | ACGGA | 3 cut(s) | 32, 576, 717 |
| TspMI | CCCGGG | 1 cut(s) | 647 |
| VpaK11BI | GGWCC | 1 cut(s) | 651 |
| XapI | RAATTY | 1 cut(s) | 24 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 181 |
| XhoI | CTCGAG | 1 cut(s) | 718 |
| XmaI | CCCGGG | 1 cut(s) | 647 |
| XmiI | GTMKAC | 1 cut(s) | 116 |
| XmnI | GAANNNNTTC | 1 cut(s) | 890 |
| XspI | CTAG | 2 cut(s) | 620, 743 |
| ZrmI | AGTACT | 1 cut(s) | 846 |