No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1176 |
| Acc65I | GGTACC | 1 cut(s) | 1213 |
| AccB1I | GGYRCC | 2 cut(s) | 1213, 1228 |
| AclWI | GGATC | 3 cut(s) | 431, 1274, 1287 |
| AcoI | YGGCCR | 2 cut(s) | 103, 437 |
| AcuI | CTGAAG | 5 cut(s) | 624, 816, 972, 1218, 1494 |
| AcvI | CACGTG | 1 cut(s) | 1330 |
| AfaI | GTAC | 5 cut(s) | 131, 170, 627, 1215, 1439 |
| AfeI | AGCGCT | 1 cut(s) | 963 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 250, 280, 435, 704, 891, 1009, 1144 |
| AflIII | ACRYGT | 1 cut(s) | 709 |
| AgeI | ACCGGT | 1 cut(s) | 1225 |
| AgsI | TTSAA | 6 cut(s) | 10, 40, 389, 803, 1097, 1337 |
| AjiI | CACGTC | 1 cut(s) | 710 |
| AjnI | CCWGG | 3 cut(s) | 1008, 1348, 1389 |
| AluBI | AGCT | 8 cut(s) | 346, 779, 872, 939, 1102, 1255, 1478, 1577 |
| AluI | AGCT | 8 cut(s) | 346, 779, 872, 939, 1102, 1255, 1478, 1577 |
| Alw21I | GWGCWC | 1 cut(s) | 1611 |
| Alw26I | GTCTC | 1 cut(s) | 508 |
| AlwI | GGATC | 3 cut(s) | 431, 1274, 1287 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1504 |
| Ama87I | CYCGRG | 4 cut(s) | 248, 377, 629, 1368 |
| Aor51HI | AGCGCT | 1 cut(s) | 963 |
| ApaI | GGGCCC | 1 cut(s) | 1397 |
| ApeKI | GCWGC | 8 cut(s) | 726, 845, 988, 1102, 1504, 1556, 1577, 1580 |
| AscI | GGCGCGCC | 1 cut(s) | 1416 |
| AseI | ATTAAT | 1 cut(s) | 207 |
| AsiGI | ACCGGT | 1 cut(s) | 1225 |
| Asp718I | GGTACC | 1 cut(s) | 1213 |
| AspA2I | CCTAGG | 1 cut(s) | 1172 |
| AspLEI | GCGC | 3 cut(s) | 964, 1418, 1420 |
| AspS9I | GGNCC | 8 cut(s) | 25, 464, 853, 1176, 1324, 1393, 1394, 1540 |
| AsuC2I | CCSGG | 2 cut(s) | 1153, 1413 |
| AsuHPI | GGTGA | 1 cut(s) | 933 |
| AvaI | CYCGRG | 4 cut(s) | 248, 377, 629, 1368 |
| AvaII | GGWCC | 1 cut(s) | 464 |
| AvrII | CCTAGG | 1 cut(s) | 1172 |
| AxyI | CCTNAGG | 1 cut(s) | 945 |
| BaeGI | GKGCMC | 1 cut(s) | 1397 |
| BalI | TGGCCA | 1 cut(s) | 439 |
| BamHI | GGATCC | 1 cut(s) | 1279 |
| BanI | GGYRCC | 2 cut(s) | 1213, 1228 |
| BanII | GRGCYC | 1 cut(s) | 1397 |
| BauI | CACGAG | 2 cut(s) | 1041, 1082 |
| BbrPI | CACGTG | 1 cut(s) | 1330 |
| BbsI | GAAGAC | 1 cut(s) | 810 |
| Bbv12I | GWGCWC | 1 cut(s) | 1611 |
| BbvI | GCAGC | 8 cut(s) | 738, 832, 1000, 1089, 1491, 1564, 1567, 1568 |
| BccI | CCATC | 5 cut(s) | 102, 454, 551, 1126, 1389 |
| BceAI | ACGGC | 1 cut(s) | 691 |
| BcgI | CGANNNNNNTGC | 8 cut(s) | 96, 130, 572, 606, 1096, 1111, 1130, 1145 |
| BciT130I | CCWGG | 3 cut(s) | 1010, 1350, 1391 |
| BciVI | GTATCC | 1 cut(s) | 573 |
| BclI | TGATCA | 1 cut(s) | 480 |
| BcnI | CCSGG | 2 cut(s) | 1153, 1413 |
| BcoDI | GTCTC | 1 cut(s) | 508 |
| BfaI | CTAG | 2 cut(s) | 623, 749 |
| BfmI | CTRYAG | 2 cut(s) | 780, 1505 |
| BfoI | RGCGCY | 1 cut(s) | 965 |
| BfuAI | ACCTGC | 1 cut(s) | 1176 |
| BfuI | GTATCC | 1 cut(s) | 573 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1553 |
| BglII | AGATCT | 1 cut(s) | 553 |
| BlnI | CCTAGG | 1 cut(s) | 1172 |
| Bme1390I | CCNGG | 5 cut(s) | 1010, 1153, 1350, 1391, 1413 |
| Bme18I | GGWCC | 1 cut(s) | 464 |
| BmeT110I | CYCGRG | 4 cut(s) | 248, 377, 629, 1368 |
| BmgBI | CACGTC | 1 cut(s) | 710 |
| BmgT120I | GGNCC | 8 cut(s) | 25, 464, 853, 1176, 1324, 1393, 1394, 1540 |
| BmrFI | CCNGG | 5 cut(s) | 1010, 1153, 1350, 1391, 1413 |
| BmsI | GCATC | 3 cut(s) | 562, 1063, 1354 |
| BpiI | GAAGAC | 1 cut(s) | 810 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 335, 367, 1362, 1394, 1437, 1469 |
| BpmI | CTGGAG | 3 cut(s) | 532, 1031, 1371 |
| BpuEI | CTTGAG | 2 cut(s) | 251, 654 |
| BpuMI | CCSGG | 2 cut(s) | 1153, 1413 |
| Bsa29I | ATCGAT | 1 cut(s) | 1071 |
| BsaAI | YACGTR | 1 cut(s) | 1330 |
| BsaBI | GATNNNNATC | 1 cut(s) | 479 |
| BsaJI | CCNNGG | 4 cut(s) | 890, 1106, 1172, 1390 |
| BsaWI | WCCGGW | 2 cut(s) | 1137, 1225 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 250, 280, 435, 704, 891, 1009, 1144 |
| Bse118I | RCCGGY | 1 cut(s) | 1225 |
| Bse1I | ACTGG | 3 cut(s) | 422, 760, 1498 |
| Bse21I | CCTNAGG | 1 cut(s) | 945 |
| Bse8I | GATNNNNATC | 1 cut(s) | 479 |
| BseBI | CCWGG | 3 cut(s) | 1010, 1350, 1391 |
| BseCI | ATCGAT | 1 cut(s) | 1071 |
| BseDI | CCNNGG | 4 cut(s) | 890, 1106, 1172, 1390 |
| BseGI | GGATG | 4 cut(s) | 134, 529, 937, 1401 |
| BseJI | GATNNNNATC | 1 cut(s) | 479 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 250, 280, 435, 704, 891, 1009, 1144 |
| BseMII | CTCAG | 7 cut(s) | 35, 321, 349, 936, 1044, 1433, 1493 |
| BseNI | ACTGG | 3 cut(s) | 422, 760, 1498 |
| BsePI | GCGCGC | 1 cut(s) | 1416 |
| BseSI | GKGCMC | 1 cut(s) | 1397 |
| BseXI | GCAGC | 8 cut(s) | 738, 832, 1000, 1089, 1491, 1564, 1567, 1568 |
| BshNI | GGYRCC | 2 cut(s) | 1213, 1228 |
| BshTI | ACCGGT | 1 cut(s) | 1225 |
| BshVI | ATCGAT | 1 cut(s) | 1071 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1611 |
| BsiHKCI | CYCGRG | 4 cut(s) | 248, 377, 629, 1368 |
| BsiSI | CCGG | 6 cut(s) | 723, 1138, 1153, 1226, 1413, 1636 |
| BslFI | GGGAC | 3 cut(s) | 477, 720, 1328 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 250, 280, 435, 704, 891, 1009, 1144 |
| BsmAI | GTCTC | 1 cut(s) | 508 |
| BsmFI | GGGAC | 3 cut(s) | 477, 720, 1328 |
| BsoBI | CYCGRG | 4 cut(s) | 248, 377, 629, 1368 |
| Bsp120I | GGGCCC | 1 cut(s) | 1393 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1397, 1611 |
| Bsp143I | GATC | 6 cut(s) | 268, 297, 423, 480, 553, 1279 |
| Bsp68I | TCGCGA | 2 cut(s) | 539, 984 |
| BspCNI | CTCAG | 7 cut(s) | 34, 320, 348, 937, 1043, 1434, 1492 |
| BspDI | ATCGAT | 1 cut(s) | 1071 |
| BspHI | TCATGA | 1 cut(s) | 1219 |
| BspMAI | CTGCAG | 1 cut(s) | 1509 |
| BspMI | ACCTGC | 1 cut(s) | 1176 |
| BspPI | GGATC | 3 cut(s) | 431, 1274, 1287 |
| BspQI | GCTCTTC | 1 cut(s) | 1092 |
| BspT107I | GGYRCC | 2 cut(s) | 1213, 1228 |
| BsrFI | RCCGGY | 1 cut(s) | 1225 |
| BsrI | ACTGG | 2 cut(s) | 422, 760 |
| BssAI | RCCGGY | 1 cut(s) | 1225 |
| BssECI | CCNNGG | 4 cut(s) | 890, 1106, 1172, 1390 |
| BssHII | GCGCGC | 1 cut(s) | 1416 |
| BssMI | GATC | 6 cut(s) | 268, 297, 423, 480, 553, 1279 |
| BssSI | CACGAG | 2 cut(s) | 1041, 1082 |
| BssT1I | CCWWGG | 1 cut(s) | 1172 |
| Bst2BI | CACGAG | 2 cut(s) | 1041, 1082 |
| Bst2UI | CCWGG | 3 cut(s) | 1010, 1350, 1391 |
| Bst4CI | ACNGT | 1 cut(s) | 1144 |
| Bst6I | CTCTTC | 4 cut(s) | 102, 539, 814, 1092 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1073 |
| BstBAI | YACGTR | 1 cut(s) | 1330 |
| BstDEI | CTNAG | 8 cut(s) | 21, 199, 307, 335, 945, 1030, 1442, 1479 |
| BstDSI | CCRYGG | 1 cut(s) | 890 |
| BstF5I | GGATG | 4 cut(s) | 134, 529, 937, 1401 |
| BstH2I | RGCGCY | 1 cut(s) | 965 |
| BstHHI | GCGC | 3 cut(s) | 964, 1418, 1420 |
| BstKTI | GATC | 6 cut(s) | 271, 300, 426, 483, 556, 1282 |
| BstMAI | GTCTC | 1 cut(s) | 508 |
| BstMBI | GATC | 6 cut(s) | 268, 297, 423, 480, 553, 1279 |
| BstNI | CCWGG | 3 cut(s) | 1010, 1350, 1391 |
| BstSCI | CCNGG | 5 cut(s) | 1008, 1151, 1348, 1389, 1411 |
| BstSFI | CTRYAG | 2 cut(s) | 780, 1505 |
| BstSLI | GKGCMC | 1 cut(s) | 1397 |
| BstV1I | GCAGC | 8 cut(s) | 738, 832, 1000, 1089, 1491, 1564, 1567, 1568 |
| BstV2I | GAAGAC | 1 cut(s) | 810 |
| BstX2I | RGATCY | 3 cut(s) | 423, 553, 1279 |
| BstYI | RGATCY | 3 cut(s) | 423, 553, 1279 |
| Bsu15I | ATCGAT | 1 cut(s) | 1071 |
| Bsu36I | CCTNAGG | 1 cut(s) | 945 |
| BsuI | GTATCC | 1 cut(s) | 573 |
| BsuTUI | ATCGAT | 1 cut(s) | 1071 |
| BtgI | CCRYGG | 1 cut(s) | 890 |
| BtrI | CACGTC | 1 cut(s) | 710 |
| BtsCI | GGATG | 4 cut(s) | 134, 529, 937, 1401 |
| BtsI | GCAGTG | 1 cut(s) | 993 |
| BtsIMutI | CAGTG | 3 cut(s) | 415, 753, 993 |
| BtuMI | TCGCGA | 2 cut(s) | 539, 984 |
| BveI | ACCTGC | 1 cut(s) | 1176 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1504 |
| CciI | TCATGA | 1 cut(s) | 1219 |
| CfoI | GCGC | 3 cut(s) | 964, 1418, 1420 |
| Cfr10I | RCCGGY | 1 cut(s) | 1225 |
| Cfr13I | GGNCC | 8 cut(s) | 25, 464, 853, 1176, 1324, 1393, 1394, 1540 |
| ClaI | ATCGAT | 1 cut(s) | 1071 |
| Csp6I | GTAC | 5 cut(s) | 130, 169, 626, 1214, 1438 |
| CspAI | ACCGGT | 1 cut(s) | 1225 |
| CviAII | CATG | 3 cut(s) | 236, 1181, 1220 |
| CviQI | GTAC | 5 cut(s) | 130, 169, 626, 1214, 1438 |
| DdeI | CTNAG | 8 cut(s) | 21, 199, 307, 335, 945, 1030, 1442, 1479 |
| DpnI | GATC | 6 cut(s) | 270, 299, 425, 482, 555, 1281 |
| DpnII | GATC | 6 cut(s) | 268, 297, 423, 480, 553, 1279 |
| EaeI | YGGCCR | 2 cut(s) | 103, 437 |
| Eam1104I | CTCTTC | 4 cut(s) | 102, 539, 814, 1092 |
| EarI | CTCTTC | 4 cut(s) | 102, 539, 814, 1092 |
| EciI | GGCGGA | 2 cut(s) | 49, 1607 |
| Eco130I | CCWWGG | 1 cut(s) | 1172 |
| Eco147I | AGGCCT | 1 cut(s) | 1377 |
| Eco24I | GRGCYC | 1 cut(s) | 1397 |
| Eco47I | GGWCC | 1 cut(s) | 464 |
| Eco47III | AGCGCT | 1 cut(s) | 963 |
| Eco57I | CTGAAG | 5 cut(s) | 624, 816, 972, 1218, 1494 |
| Eco72I | CACGTG | 1 cut(s) | 1330 |
| Eco81I | CCTNAGG | 1 cut(s) | 945 |
| Eco88I | CYCGRG | 4 cut(s) | 248, 377, 629, 1368 |
| EcoO109I | RGGNCCY | 4 cut(s) | 25, 464, 1176, 1394 |
| EcoRII | CCWGG | 3 cut(s) | 1008, 1348, 1389 |
| EcoT14I | CCWWGG | 1 cut(s) | 1172 |
| EcoT38I | GRGCYC | 1 cut(s) | 1397 |
| ErhI | CCWWGG | 1 cut(s) | 1172 |
| FaeI | CATG | 3 cut(s) | 239, 1184, 1223 |
| FaqI | GGGAC | 3 cut(s) | 477, 720, 1328 |
| FatI | CATG | 3 cut(s) | 235, 1180, 1219 |
| FauI | CCCGC | 2 cut(s) | 147, 228 |
| FbaI | TGATCA | 1 cut(s) | 480 |
| FokI | GGATG | 4 cut(s) | 121, 536, 944, 1388 |
| FriOI | GRGCYC | 1 cut(s) | 1397 |
| FspBI | CTAG | 5 cut(s) | 623, 749, 1173, 1313, 1601 |
| GlaI | GCGC | 3 cut(s) | 963, 1417, 1419 |
| GsuI | CTGGAG | 3 cut(s) | 532, 1031, 1371 |
| HaeII | RGCGCY | 1 cut(s) | 965 |
| HapII | CCGG | 6 cut(s) | 723, 1138, 1153, 1226, 1413, 1636 |
| HhaI | GCGC | 3 cut(s) | 964, 1418, 1420 |
| Hin1II | CATG | 3 cut(s) | 239, 1184, 1223 |
| Hin6I | GCGC | 3 cut(s) | 962, 1416, 1418 |
| HinP1I | GCGC | 3 cut(s) | 962, 1416, 1418 |
| HincII | GTYRAC | 2 cut(s) | 45, 505 |
| HindII | GTYRAC | 2 cut(s) | 45, 505 |
| HinfI | GANTC | 5 cut(s) | 36, 216, 806, 1028, 1428 |
| HpaI | GTTAAC | 1 cut(s) | 45 |
| HpaII | CCGG | 6 cut(s) | 723, 1138, 1153, 1226, 1413, 1636 |
| HphI | GGTGA | 1 cut(s) | 933 |
| Hpy166II | GTNNAC | 3 cut(s) | 45, 132, 505 |
| Hpy188I | TCNGA | 4 cut(s) | 151, 310, 471, 820 |
| Hpy8I | GTNNAC | 3 cut(s) | 45, 132, 505 |
| Hpy99I | CGWCG | 3 cut(s) | 70, 294, 326 |
| HpyAV | CCTTC | 5 cut(s) | 182, 254, 729, 1298, 1367 |
| HpyCH4III | ACNGT | 1 cut(s) | 1144 |
| HpyCH4IV | ACGT | 2 cut(s) | 709, 1329 |
| HpyCH4V | TGCA | 4 cut(s) | 415, 998, 1507, 1631 |
| HpyF3I | CTNAG | 8 cut(s) | 21, 199, 307, 335, 945, 1030, 1442, 1479 |
| HpySE526I | ACGT | 2 cut(s) | 709, 1329 |
| Hsp92II | CATG | 3 cut(s) | 239, 1184, 1223 |
| HspAI | GCGC | 3 cut(s) | 962, 1416, 1418 |
| KpnI | GGTACC | 1 cut(s) | 1217 |
| Ksp22I | TGATCA | 1 cut(s) | 480 |
| KspAI | GTTAAC | 1 cut(s) | 45 |
| Kzo9I | GATC | 6 cut(s) | 268, 297, 423, 480, 553, 1279 |
| LguI | GCTCTTC | 1 cut(s) | 1092 |
| LmnI | GCTCC | 5 cut(s) | 643, 1012, 1081, 1252, 1463 |
| Lsp1109I | GCAGC | 8 cut(s) | 738, 832, 1000, 1089, 1491, 1564, 1567, 1568 |
| LweI | GCATC | 3 cut(s) | 562, 1063, 1354 |
| MaeI | CTAG | 5 cut(s) | 623, 749, 1173, 1313, 1601 |
| MaeII | ACGT | 2 cut(s) | 709, 1329 |
| MaeIII | GTNAC | 1 cut(s) | 1086 |
| MalI | GATC | 6 cut(s) | 270, 299, 425, 482, 555, 1281 |
| MboI | GATC | 6 cut(s) | 268, 297, 423, 480, 553, 1279 |
| MfeI | CAATTG | 2 cut(s) | 433, 1061 |
| MflI | RGATCY | 3 cut(s) | 423, 553, 1279 |
| MhlI | GDGCHC | 2 cut(s) | 1397, 1611 |
| MlsI | TGGCCA | 1 cut(s) | 439 |
| MluCI | AATT | 6 cut(s) | 259, 384, 392, 433, 924, 1061 |
| MluNI | TGGCCA | 1 cut(s) | 439 |
| MlyI | GAGTC | 3 cut(s) | 800, 1022, 1437 |
| Mox20I | TGGCCA | 1 cut(s) | 439 |
| MscI | TGGCCA | 1 cut(s) | 439 |
| MseI | TTAA | 5 cut(s) | 44, 207, 258, 858, 1307 |
| Msp20I | TGGCCA | 1 cut(s) | 439 |
| MspA1I | CMGCKG | 1 cut(s) | 845 |
| MspI | CCGG | 6 cut(s) | 723, 1138, 1153, 1226, 1413, 1636 |
| MspR9I | CCNGG | 5 cut(s) | 1010, 1153, 1350, 1391, 1413 |
| MunI | CAATTG | 2 cut(s) | 433, 1061 |
| MvaI | CCWGG | 3 cut(s) | 1010, 1350, 1391 |
| NciI | CCSGG | 2 cut(s) | 1153, 1413 |
| NdeII | GATC | 6 cut(s) | 268, 297, 423, 480, 553, 1279 |
| NlaIII | CATG | 3 cut(s) | 239, 1184, 1223 |
| NmeAIII | GCCGAG | 1 cut(s) | 1131 |
| NruI | TCGCGA | 2 cut(s) | 539, 984 |
| PaeR7I | CTCGAG | 1 cut(s) | 1368 |
| PagI | TCATGA | 1 cut(s) | 1219 |
| PalAI | GGCGCGCC | 1 cut(s) | 1416 |
| PauI | GCGCGC | 1 cut(s) | 1416 |
| PceI | AGGCCT | 1 cut(s) | 1377 |
| PciSI | GCTCTTC | 1 cut(s) | 1092 |
| PfeI | GAWTC | 2 cut(s) | 36, 216 |
| PfoI | TCCNGGA | 1 cut(s) | 1008 |
| PinAI | ACCGGT | 1 cut(s) | 1225 |
| PleI | GAGTC | 3 cut(s) | 800, 1022, 1436 |
| PmaCI | CACGTG | 1 cut(s) | 1330 |
| PmlI | CACGTG | 1 cut(s) | 1330 |
| PpsI | GAGTC | 3 cut(s) | 800, 1022, 1436 |
| Ppu21I | YACGTR | 1 cut(s) | 1330 |
| PpuMI | RGGWCCY | 1 cut(s) | 464 |
| PshBI | ATTAAT | 1 cut(s) | 207 |
| Psp5II | RGGWCCY | 1 cut(s) | 464 |
| Psp6I | CCWGG | 3 cut(s) | 1008, 1348, 1389 |
| PspCI | CACGTG | 1 cut(s) | 1330 |
| PspGI | CCWGG | 3 cut(s) | 1008, 1348, 1389 |
| PspOMI | GGGCCC | 1 cut(s) | 1393 |
| PspPI | GGNCC | 8 cut(s) | 25, 464, 853, 1176, 1324, 1393, 1394, 1540 |
| PspPPI | RGGWCCY | 1 cut(s) | 464 |
| PstI | CTGCAG | 1 cut(s) | 1509 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1504 |
| PsuI | RGATCY | 3 cut(s) | 423, 553, 1279 |
| PteI | GCGCGC | 1 cut(s) | 1416 |
| RruI | TCGCGA | 2 cut(s) | 539, 984 |
| RsaI | GTAC | 5 cut(s) | 131, 170, 627, 1215, 1439 |
| RsaNI | GTAC | 5 cut(s) | 130, 169, 626, 1214, 1438 |
| SapI | GCTCTTC | 1 cut(s) | 1092 |
| SaqAI | TTAA | 5 cut(s) | 44, 207, 258, 858, 1307 |
| Sau3AI | GATC | 6 cut(s) | 268, 297, 423, 480, 553, 1279 |
| Sau96I | GGNCC | 8 cut(s) | 25, 464, 853, 1176, 1324, 1393, 1394, 1540 |
| SchI | GAGTC | 3 cut(s) | 800, 1022, 1437 |
| ScrFI | CCNGG | 5 cut(s) | 1010, 1153, 1350, 1391, 1413 |
| SduI | GDGCHC | 2 cut(s) | 1397, 1611 |
| SfaNI | GCATC | 3 cut(s) | 562, 1063, 1354 |
| SfcI | CTRYAG | 2 cut(s) | 780, 1505 |
| Sfr274I | CTCGAG | 1 cut(s) | 1368 |
| SgsI | GGCGCGCC | 1 cut(s) | 1416 |
| SinI | GGWCC | 1 cut(s) | 464 |
| SlaI | CTCGAG | 1 cut(s) | 1368 |
| SmlI | CTYRAG | 3 cut(s) | 230, 669, 1368 |
| SmoI | CTYRAG | 3 cut(s) | 230, 669, 1368 |
| Sse9I | AATT | 6 cut(s) | 259, 384, 392, 433, 924, 1061 |
| SseBI | AGGCCT | 1 cut(s) | 1377 |
| SspMI | CTAG | 5 cut(s) | 623, 749, 1173, 1313, 1601 |
| StuI | AGGCCT | 1 cut(s) | 1377 |
| StyD4I | CCNGG | 5 cut(s) | 1008, 1151, 1348, 1389, 1411 |
| StyI | CCWWGG | 1 cut(s) | 1172 |
| TaaI | ACNGT | 1 cut(s) | 1144 |
| TaiI | ACGT | 2 cut(s) | 712, 1332 |
| TaqI | TCGA | 7 cut(s) | 195, 868, 919, 1071, 1121, 1369, 1518 |
| TaqII | GACCGA | 1 cut(s) | 899 |
| TasI | AATT | 6 cut(s) | 259, 384, 392, 433, 924, 1061 |
| TauI | GCSGC | 7 cut(s) | 65, 81, 120, 190, 732, 845, 1514 |
| TfiI | GAWTC | 2 cut(s) | 36, 216 |
| Tru1I | TTAA | 5 cut(s) | 44, 207, 258, 858, 1307 |
| Tru9I | TTAA | 5 cut(s) | 44, 207, 258, 858, 1307 |
| TscAI | CASTG | 3 cut(s) | 422, 760, 1000 |
| TseI | GCWGC | 8 cut(s) | 726, 845, 988, 1102, 1504, 1556, 1577, 1580 |
| TspDTI | ATGAA | 5 cut(s) | 41, 543, 918, 1236, 1474 |
| TspRI | CASTG | 3 cut(s) | 422, 760, 1000 |
| VpaK11BI | GGWCC | 1 cut(s) | 464 |
| VspI | ATTAAT | 1 cut(s) | 207 |
| XhoI | CTCGAG | 1 cut(s) | 1368 |
| XmaJI | CCTAGG | 1 cut(s) | 1172 |
| XspI | CTAG | 5 cut(s) | 623, 749, 1173, 1313, 1601 |