Plant mobile domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 39 |
| Acc65I | GGTACC | 2 cut(s) | 1275, 1469 |
| AccB1I | GGYRCC | 6 cut(s) | 385, 686, 1240, 1275, 1405, 1469 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1401, 1466 |
| AccI | GTMKAC | 3 cut(s) | 509, 521, 604 |
| AccII | CGCG | 7 cut(s) | 143, 684, 1140, 1613, 1670, 1672, 1710 |
| AccIII | TCCGGA | 1 cut(s) | 482 |
| AclWI | GGATC | 5 cut(s) | 91, 952, 965, 1094, 1107 |
| AcoI | YGGCCR | 5 cut(s) | 634, 834, 853, 871, 1001 |
| AcsI | RAATTY | 1 cut(s) | 1515 |
| AcuI | CTGAAG | 1 cut(s) | 98 |
| AcyI | GRCGYC | 1 cut(s) | 687 |
| AfaI | GTAC | 6 cut(s) | 770, 1050, 1277, 1471, 1481, 1653 |
| AflIII | ACRYGT | 1 cut(s) | 1648 |
| AgsI | TTSAA | 3 cut(s) | 1079, 1571, 1691 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 474 |
| AhlI | ACTAGT | 1 cut(s) | 599 |
| AjiI | CACGTC | 1 cut(s) | 1682 |
| AjnI | CCWGG | 7 cut(s) | 548, 821, 858, 1394, 1400, 1453, 1472 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1071, 1103 |
| AluBI | AGCT | 3 cut(s) | 1172, 1595, 1694 |
| AluI | AGCT | 3 cut(s) | 1172, 1595, 1694 |
| Alw21I | GWGCWC | 1 cut(s) | 738 |
| Alw26I | GTCTC | 2 cut(s) | 248, 1312 |
| AlwI | GGATC | 5 cut(s) | 91, 952, 965, 1094, 1107 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1401 |
| Ama87I | CYCGRG | 3 cut(s) | 128, 1094, 1409 |
| Aor13HI | TCCGGA | 1 cut(s) | 482 |
| ApeKI | GCWGC | 4 cut(s) | 45, 358, 1169, 1534 |
| ApoI | RAATTY | 1 cut(s) | 1515 |
| AseI | ATTAAT | 1 cut(s) | 204 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1063 |
| Asp718I | GGTACC | 2 cut(s) | 1275, 1469 |
| AspA2I | CCTAGG | 2 cut(s) | 121, 368 |
| AspLEI | GCGC | 4 cut(s) | 689, 892, 1142, 1672 |
| AspS9I | GGNCC | 7 cut(s) | 459, 818, 947, 1042, 1398, 1431, 1457 |
| AsuC2I | CCSGG | 3 cut(s) | 1273, 1410, 1411 |
| AsuHPI | GGTGA | 5 cut(s) | 17, 390, 488, 565, 1059 |
| AvaI | CYCGRG | 3 cut(s) | 128, 1094, 1409 |
| AvaII | GGWCC | 3 cut(s) | 459, 818, 1042 |
| AvrII | CCTAGG | 2 cut(s) | 121, 368 |
| BaeGI | GKGCMC | 2 cut(s) | 390, 1410 |
| BalI | TGGCCA | 3 cut(s) | 636, 836, 873 |
| BamHI | GGATCC | 2 cut(s) | 957, 1099 |
| BanI | GGYRCC | 6 cut(s) | 385, 686, 1240, 1275, 1405, 1469 |
| BanII | GRGCYC | 2 cut(s) | 109, 1493 |
| BbsI | GAAGAC | 1 cut(s) | 36 |
| Bbv12I | GWGCWC | 1 cut(s) | 738 |
| BbvCI | CCTCAGC | 1 cut(s) | 1623 |
| BbvI | GCAGC | 4 cut(s) | 57, 345, 1181, 1546 |
| BceAI | ACGGC | 3 cut(s) | 631, 868, 1645 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 340, 374 |
| BciT130I | CCWGG | 7 cut(s) | 550, 823, 860, 1396, 1402, 1455, 1474 |
| BclI | TGATCA | 1 cut(s) | 157 |
| BcnI | CCSGG | 3 cut(s) | 1273, 1410, 1411 |
| BcoDI | GTCTC | 2 cut(s) | 248, 1312 |
| BcuI | ACTAGT | 1 cut(s) | 599 |
| BfaI | CTAG | 9 cut(s) | 122, 369, 581, 600, 764, 884, 1046, 1104, 1500 |
| BfoI | RGCGCY | 1 cut(s) | 690 |
| BfuAI | ACCTGC | 1 cut(s) | 39 |
| BisI | GCNGC | 8 cut(s) | 46, 359, 682, 685, 1138, 1170, 1497, 1535 |
| BlnI | CCTAGG | 2 cut(s) | 121, 368 |
| BlpI | GCTNAGC | 1 cut(s) | 1492 |
| BlsI | GCNGC | 8 cut(s) | 47, 360, 683, 686, 1139, 1171, 1498, 1536 |
| BmcAI | AGTACT | 2 cut(s) | 770, 1050 |
| Bme18I | GGWCC | 3 cut(s) | 459, 818, 1042 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 474 |
| BmeT110I | CYCGRG | 3 cut(s) | 128, 1094, 1409 |
| BmgBI | CACGTC | 1 cut(s) | 1682 |
| BmgT120I | GGNCC | 7 cut(s) | 459, 818, 947, 1042, 1398, 1431, 1457 |
| BmsI | GCATC | 3 cut(s) | 389, 931, 1427 |
| BpiI | GAAGAC | 1 cut(s) | 36 |
| BpmI | CTGGAG | 2 cut(s) | 223, 571 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1623 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1492 |
| BpuMI | CCSGG | 3 cut(s) | 1273, 1410, 1411 |
| Bsa29I | ATCGAT | 1 cut(s) | 329 |
| BsaAI | YACGTR | 1 cut(s) | 628 |
| BsaHI | GRCGYC | 1 cut(s) | 687 |
| BsaWI | WCCGGW | 1 cut(s) | 482 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 683, 713 |
| Bse1I | ACTGG | 5 cut(s) | 240, 531, 751, 776, 820 |
| Bse3DI | GCAATG | 1 cut(s) | 113 |
| BseAI | TCCGGA | 1 cut(s) | 482 |
| BseBI | CCWGG | 7 cut(s) | 550, 823, 860, 1396, 1402, 1455, 1474 |
| BseCI | ATCGAT | 1 cut(s) | 329 |
| BseGI | GGATG | 4 cut(s) | 279, 922, 1549, 1637 |
| BseMI | GCAATG | 1 cut(s) | 113 |
| BseMII | CTCAG | 4 cut(s) | 301, 443, 1506, 1614 |
| BseNI | ACTGG | 5 cut(s) | 240, 531, 751, 776, 820 |
| BseRI | GAGGAG | 4 cut(s) | 226, 352, 355, 417 |
| BseSI | GKGCMC | 2 cut(s) | 390, 1410 |
| BseXI | GCAGC | 4 cut(s) | 57, 345, 1181, 1546 |
| BseYI | CCCAGC | 1 cut(s) | 455 |
| BsgI | GTGCAG | 1 cut(s) | 778 |
| Bsh1236I | CGCG | 7 cut(s) | 143, 684, 1140, 1613, 1670, 1672, 1710 |
| BshNI | GGYRCC | 6 cut(s) | 385, 686, 1240, 1275, 1405, 1469 |
| BshVI | ATCGAT | 1 cut(s) | 329 |
| BsiHKAI | GWGCWC | 1 cut(s) | 738 |
| BsiHKCI | CYCGRG | 3 cut(s) | 128, 1094, 1409 |
| BsiSI | CCGG | 3 cut(s) | 483, 1272, 1410 |
| BslFI | GGGAC | 2 cut(s) | 804, 1341 |
| BsmAI | GTCTC | 2 cut(s) | 248, 1312 |
| BsmFI | GGGAC | 2 cut(s) | 804, 1341 |
| BsmI | GAATGC | 2 cut(s) | 360, 1294 |
| BsoBI | CYCGRG | 3 cut(s) | 128, 1094, 1409 |
| Bsp1286I | GDGCHC | 5 cut(s) | 109, 390, 738, 1410, 1493 |
| Bsp13I | TCCGGA | 1 cut(s) | 482 |
| Bsp1407I | TGTACA | 1 cut(s) | 1651 |
| Bsp143I | GATC | 8 cut(s) | 83, 132, 157, 330, 344, 957, 1099, 1114 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1492 |
| Bsp19I | CCATGG | 1 cut(s) | 1460 |
| BspCNI | CTCAG | 4 cut(s) | 300, 442, 1505, 1615 |
| BspDI | ATCGAT | 1 cut(s) | 329 |
| BspEI | TCCGGA | 1 cut(s) | 482 |
| BspFNI | CGCG | 7 cut(s) | 143, 684, 1140, 1613, 1670, 1672, 1710 |
| BspMI | ACCTGC | 1 cut(s) | 39 |
| BspPI | GGATC | 5 cut(s) | 91, 952, 965, 1094, 1107 |
| BspT107I | GGYRCC | 6 cut(s) | 385, 686, 1240, 1275, 1405, 1469 |
| BsrDI | GCAATG | 1 cut(s) | 113 |
| BsrGI | TGTACA | 1 cut(s) | 1651 |
| BsrI | ACTGG | 5 cut(s) | 240, 531, 751, 776, 820 |
| BssMI | GATC | 8 cut(s) | 83, 132, 157, 330, 344, 957, 1099, 1114 |
| BssNI | GRCGYC | 1 cut(s) | 687 |
| BssT1I | CCWWGG | 4 cut(s) | 121, 368, 868, 1460 |
| Bst2UI | CCWGG | 7 cut(s) | 550, 823, 860, 1396, 1402, 1455, 1474 |
| Bst4CI | ACNGT | 2 cut(s) | 410, 711 |
| BstACI | GRCGYC | 1 cut(s) | 687 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 64 |
| BstAUI | TGTACA | 1 cut(s) | 1651 |
| BstBAI | YACGTR | 1 cut(s) | 628 |
| BstDEI | CTNAG | 4 cut(s) | 287, 429, 1492, 1623 |
| BstDSI | CCRYGG | 3 cut(s) | 682, 707, 1460 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 882 |
| BstF5I | GGATG | 4 cut(s) | 279, 922, 1549, 1637 |
| BstFNI | CGCG | 7 cut(s) | 143, 684, 1140, 1613, 1670, 1672, 1710 |
| BstH2I | RGCGCY | 1 cut(s) | 690 |
| BstHHI | GCGC | 4 cut(s) | 689, 892, 1142, 1672 |
| BstKTI | GATC | 8 cut(s) | 86, 135, 160, 333, 347, 960, 1102, 1117 |
| BstMAI | GTCTC | 2 cut(s) | 248, 1312 |
| BstMBI | GATC | 8 cut(s) | 83, 132, 157, 330, 344, 957, 1099, 1114 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 64, 628, 945, 1384, 1389, 1405, 1619 |
| BstNI | CCWGG | 7 cut(s) | 550, 823, 860, 1396, 1402, 1455, 1474 |
| BstNSI | RCATGY | 2 cut(s) | 1652, 1658 |
| BstSLI | GKGCMC | 2 cut(s) | 390, 1410 |
| BstUI | CGCG | 7 cut(s) | 143, 684, 1140, 1613, 1670, 1672, 1710 |
| BstV1I | GCAGC | 4 cut(s) | 57, 345, 1181, 1546 |
| BstV2I | GAAGAC | 1 cut(s) | 36 |
| BstX2I | RGATCY | 3 cut(s) | 83, 957, 1099 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1461 |
| BstYI | RGATCY | 3 cut(s) | 83, 957, 1099 |
| Bsu15I | ATCGAT | 1 cut(s) | 329 |
| BsuTUI | ATCGAT | 1 cut(s) | 329 |
| BtgI | CCRYGG | 3 cut(s) | 682, 707, 1460 |
| BtrI | CACGTC | 1 cut(s) | 1682 |
| BtsCI | GGATG | 4 cut(s) | 279, 922, 1549, 1637 |
| BtsI | GCAGTG | 2 cut(s) | 390, 1013 |
| BtsIMutI | CAGTG | 3 cut(s) | 390, 813, 1013 |
| BveI | ACCTGC | 1 cut(s) | 39 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1401 |
| CfoI | GCGC | 4 cut(s) | 689, 892, 1142, 1672 |
| Cfr13I | GGNCC | 7 cut(s) | 459, 818, 947, 1042, 1398, 1431, 1457 |
| Cfr42I | CCGCGG | 1 cut(s) | 685 |
| Cfr9I | CCCGGG | 1 cut(s) | 1409 |
| ClaI | ATCGAT | 1 cut(s) | 329 |
| CseI | GACGC | 1 cut(s) | 498 |
| CsiI | ACCWGGT | 1 cut(s) | 858 |
| Csp6I | GTAC | 6 cut(s) | 769, 1049, 1276, 1470, 1480, 1652 |
| CviAII | CATG | 6 cut(s) | 632, 1082, 1438, 1461, 1649, 1655 |
| CviQI | GTAC | 6 cut(s) | 769, 1049, 1276, 1470, 1480, 1652 |
| DdeI | CTNAG | 4 cut(s) | 287, 429, 1492, 1623 |
| DinI | GGCGCC | 1 cut(s) | 688 |
| DpnI | GATC | 8 cut(s) | 85, 134, 159, 332, 346, 959, 1101, 1116 |
| DpnII | GATC | 8 cut(s) | 83, 132, 157, 330, 344, 957, 1099, 1114 |
| DriI | GACNNNNNGTC | 1 cut(s) | 474 |
| EaeI | YGGCCR | 5 cut(s) | 634, 834, 853, 871, 1001 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 474 |
| Eco130I | CCWWGG | 4 cut(s) | 121, 368, 868, 1460 |
| Eco147I | AGGCCT | 3 cut(s) | 126, 419, 867 |
| Eco24I | GRGCYC | 2 cut(s) | 109, 1493 |
| Eco32I | GATATC | 2 cut(s) | 586, 1583 |
| Eco47I | GGWCC | 3 cut(s) | 459, 818, 1042 |
| Eco57I | CTGAAG | 1 cut(s) | 98 |
| Eco88I | CYCGRG | 3 cut(s) | 128, 1094, 1409 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 882 |
| EcoO109I | RGGNCCY | 2 cut(s) | 459, 1398 |
| EcoRII | CCWGG | 7 cut(s) | 548, 821, 858, 1394, 1400, 1453, 1472 |
| EcoRV | GATATC | 2 cut(s) | 586, 1583 |
| EcoT14I | CCWWGG | 4 cut(s) | 121, 368, 868, 1460 |
| EcoT22I | ATGCAT | 1 cut(s) | 924 |
| EcoT38I | GRGCYC | 2 cut(s) | 109, 1493 |
| EgeI | GGCGCC | 1 cut(s) | 688 |
| EheI | GGCGCC | 1 cut(s) | 688 |
| ErhI | CCWWGG | 4 cut(s) | 121, 368, 868, 1460 |
| FaeI | CATG | 6 cut(s) | 635, 1085, 1441, 1464, 1652, 1658 |
| FaqI | GGGAC | 2 cut(s) | 804, 1341 |
| FatI | CATG | 6 cut(s) | 631, 1081, 1437, 1460, 1648, 1654 |
| FauI | CCCGC | 4 cut(s) | 148, 1080, 1606, 1675 |
| FauNDI | CATATG | 1 cut(s) | 1425 |
| FbaI | TGATCA | 1 cut(s) | 157 |
| FblI | GTMKAC | 3 cut(s) | 509, 521, 604 |
| Fnu4HI | GCNGC | 8 cut(s) | 46, 359, 682, 685, 1138, 1170, 1497, 1535 |
| FokI | GGATG | 4 cut(s) | 266, 909, 1556, 1624 |
| FriOI | GRGCYC | 2 cut(s) | 109, 1493 |
| Fsp4HI | GCNGC | 8 cut(s) | 46, 359, 682, 685, 1138, 1170, 1497, 1535 |
| FspBI | CTAG | 9 cut(s) | 122, 369, 581, 600, 764, 884, 1046, 1104, 1500 |
| GlaI | GCGC | 4 cut(s) | 688, 891, 1141, 1671 |
| GluI | GCNGC | 8 cut(s) | 46, 359, 682, 685, 1138, 1170, 1497, 1535 |
| GsaI | CCCAGC | 1 cut(s) | 459 |
| GsuI | CTGGAG | 2 cut(s) | 223, 571 |
| HaeII | RGCGCY | 1 cut(s) | 690 |
| HapII | CCGG | 3 cut(s) | 483, 1272, 1410 |
| HgaI | GACGC | 1 cut(s) | 498 |
| HhaI | GCGC | 4 cut(s) | 689, 892, 1142, 1672 |
| Hin1I | GRCGYC | 1 cut(s) | 687 |
| Hin1II | CATG | 6 cut(s) | 635, 1085, 1441, 1464, 1652, 1658 |
| Hin6I | GCGC | 4 cut(s) | 687, 890, 1140, 1670 |
| HinP1I | GCGC | 4 cut(s) | 687, 890, 1140, 1670 |
| HincII | GTYRAC | 1 cut(s) | 522 |
| HindII | GTYRAC | 1 cut(s) | 522 |
| HinfI | GANTC | 7 cut(s) | 433, 644, 654, 670, 698, 1305, 1698 |
| HpaII | CCGG | 3 cut(s) | 483, 1272, 1410 |
| HphI | GGTGA | 5 cut(s) | 17, 390, 488, 565, 1059 |
| Hpy166II | GTNNAC | 6 cut(s) | 8, 510, 522, 605, 982, 1451 |
| Hpy188I | TCNGA | 7 cut(s) | 137, 230, 290, 432, 540, 653, 675 |
| Hpy8I | GTNNAC | 6 cut(s) | 8, 510, 522, 605, 982, 1451 |
| Hpy99I | CGWCG | 1 cut(s) | 617 |
| HpyAV | CCTTC | 5 cut(s) | 73, 669, 937, 961, 1100 |
| HpyCH4III | ACNGT | 2 cut(s) | 410, 711 |
| HpyCH4IV | ACGT | 2 cut(s) | 627, 1681 |
| HpyCH4V | TGCA | 6 cut(s) | 631, 780, 795, 922, 976, 1169 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 64, 628, 945, 1384, 1389, 1405, 1619 |
| HpyF3I | CTNAG | 4 cut(s) | 287, 429, 1492, 1623 |
| HpySE526I | ACGT | 2 cut(s) | 627, 1681 |
| Hsp92I | GRCGYC | 1 cut(s) | 687 |
| Hsp92II | CATG | 6 cut(s) | 635, 1085, 1441, 1464, 1652, 1658 |
| HspAI | GCGC | 4 cut(s) | 687, 890, 1140, 1670 |
| KasI | GGCGCC | 1 cut(s) | 686 |
| Kpn2I | TCCGGA | 1 cut(s) | 482 |
| KpnI | GGTACC | 2 cut(s) | 1279, 1473 |
| Ksp22I | TGATCA | 1 cut(s) | 157 |
| KspI | CCGCGG | 1 cut(s) | 685 |
| Kzo9I | GATC | 8 cut(s) | 83, 132, 157, 330, 344, 957, 1099, 1114 |
| LmnI | GCTCC | 2 cut(s) | 733, 1157 |
| Lsp1109I | GCAGC | 4 cut(s) | 57, 345, 1181, 1546 |
| LweI | GCATC | 3 cut(s) | 389, 931, 1427 |
| MabI | ACCWGGT | 1 cut(s) | 858 |
| MaeI | CTAG | 9 cut(s) | 122, 369, 581, 600, 764, 884, 1046, 1104, 1500 |
| MaeII | ACGT | 2 cut(s) | 627, 1681 |
| MaeIII | GTNAC | 2 cut(s) | 13, 151 |
| MalI | GATC | 8 cut(s) | 85, 134, 159, 332, 346, 959, 1101, 1116 |
| MboI | GATC | 8 cut(s) | 83, 132, 157, 330, 344, 957, 1099, 1114 |
| MboII | GAAGA | 6 cut(s) | 41, 792, 989, 1071, 1329, 1559 |
| MfeI | CAATTG | 2 cut(s) | 1235, 1525 |
| MflI | RGATCY | 3 cut(s) | 83, 957, 1099 |
| MhlI | GDGCHC | 5 cut(s) | 109, 390, 738, 1410, 1493 |
| MlsI | TGGCCA | 3 cut(s) | 636, 836, 873 |
| MluCI | AATT | 6 cut(s) | 36, 638, 1183, 1235, 1515, 1525 |
| MluNI | TGGCCA | 3 cut(s) | 636, 836, 873 |
| Mly113I | GGCGCC | 1 cut(s) | 687 |
| MlyI | GAGTC | 4 cut(s) | 427, 638, 692, 1314 |
| MmeI | TCCRAC | 1 cut(s) | 1179 |
| Mox20I | TGGCCA | 3 cut(s) | 636, 836, 873 |
| Mph1103I | ATGCAT | 1 cut(s) | 924 |
| MroI | TCCGGA | 1 cut(s) | 482 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1063 |
| MscI | TGGCCA | 3 cut(s) | 636, 836, 873 |
| MseI | TTAA | 5 cut(s) | 35, 204, 248, 1506, 1512 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1653 |
| Msp20I | TGGCCA | 3 cut(s) | 636, 836, 873 |
| MspA1I | CMGCKG | 2 cut(s) | 684, 1172 |
| MspI | CCGG | 3 cut(s) | 483, 1272, 1410 |
| MunI | CAATTG | 2 cut(s) | 1235, 1525 |
| Mva1269I | GAATGC | 2 cut(s) | 360, 1294 |
| MvaI | CCWGG | 7 cut(s) | 550, 823, 860, 1396, 1402, 1455, 1474 |
| MvnI | CGCG | 7 cut(s) | 143, 684, 1140, 1613, 1670, 1672, 1710 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 64, 628, 945, 1384, 1389, 1405, 1619 |
| NarI | GGCGCC | 1 cut(s) | 687 |
| NciI | CCSGG | 3 cut(s) | 1273, 1410, 1411 |
| NcoI | CCATGG | 1 cut(s) | 1460 |
| NdeI | CATATG | 1 cut(s) | 1425 |
| NdeII | GATC | 8 cut(s) | 83, 132, 157, 330, 344, 957, 1099, 1114 |
| NlaIII | CATG | 6 cut(s) | 635, 1085, 1441, 1464, 1652, 1658 |
| NsiI | ATGCAT | 1 cut(s) | 924 |
| NspI | RCATGY | 2 cut(s) | 1652, 1658 |
| PaeR7I | CTCGAG | 1 cut(s) | 128 |
| PasI | CCCWGGG | 1 cut(s) | 1401 |
| PceI | AGGCCT | 3 cut(s) | 126, 419, 867 |
| PciI | ACATGT | 1 cut(s) | 1648 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1707 |
| PctI | GAATGC | 2 cut(s) | 360, 1294 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1063 |
| PfeI | GAWTC | 3 cut(s) | 654, 670, 1698 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1401, 1466 |
| PkrI | GCNGC | 8 cut(s) | 47, 360, 683, 686, 1139, 1171, 1498, 1536 |
| PleI | GAGTC | 4 cut(s) | 427, 638, 692, 1313 |
| PluTI | GGCGCC | 1 cut(s) | 690 |
| PpsI | GAGTC | 4 cut(s) | 427, 638, 692, 1313 |
| Ppu21I | YACGTR | 1 cut(s) | 628 |
| PpuMI | RGGWCCY | 1 cut(s) | 459 |
| PscI | ACATGT | 1 cut(s) | 1648 |
| PshBI | ATTAAT | 1 cut(s) | 204 |
| Psp5II | RGGWCCY | 1 cut(s) | 459 |
| Psp6I | CCWGG | 7 cut(s) | 548, 821, 858, 1394, 1400, 1453, 1472 |
| PspFI | CCCAGC | 1 cut(s) | 455 |
| PspGI | CCWGG | 7 cut(s) | 548, 821, 858, 1394, 1400, 1453, 1472 |
| PspPI | GGNCC | 7 cut(s) | 459, 818, 947, 1042, 1398, 1431, 1457 |
| PspPPI | RGGWCCY | 1 cut(s) | 459 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1401 |
| PsuI | RGATCY | 3 cut(s) | 83, 957, 1099 |
| PvuII | CAGCTG | 1 cut(s) | 1172 |
| RsaI | GTAC | 6 cut(s) | 770, 1050, 1277, 1471, 1481, 1653 |
| RsaNI | GTAC | 6 cut(s) | 769, 1049, 1276, 1470, 1480, 1652 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1653 |
| SacII | CCGCGG | 1 cut(s) | 685 |
| SalI | GTCGAC | 1 cut(s) | 520 |
| SaqAI | TTAA | 5 cut(s) | 35, 204, 248, 1506, 1512 |
| SatI | GCNGC | 8 cut(s) | 46, 359, 682, 685, 1138, 1170, 1497, 1535 |
| Sau3AI | GATC | 8 cut(s) | 83, 132, 157, 330, 344, 957, 1099, 1114 |
| Sau96I | GGNCC | 7 cut(s) | 459, 818, 947, 1042, 1398, 1431, 1457 |
| ScaI | AGTACT | 2 cut(s) | 770, 1050 |
| SchI | GAGTC | 4 cut(s) | 427, 638, 692, 1314 |
| SduI | GDGCHC | 5 cut(s) | 109, 390, 738, 1410, 1493 |
| SexAI | ACCWGGT | 1 cut(s) | 858 |
| SfaNI | GCATC | 3 cut(s) | 389, 931, 1427 |
| SfoI | GGCGCC | 1 cut(s) | 688 |
| Sfr274I | CTCGAG | 1 cut(s) | 128 |
| Sfr303I | CCGCGG | 1 cut(s) | 685 |
| SgrBI | CCGCGG | 1 cut(s) | 685 |
| SinI | GGWCC | 3 cut(s) | 459, 818, 1042 |
| SlaI | CTCGAG | 1 cut(s) | 128 |
| SmaI | CCCGGG | 1 cut(s) | 1411 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1653 |
| SmlI | CTYRAG | 1 cut(s) | 128 |
| SmoI | CTYRAG | 1 cut(s) | 128 |
| SpeI | ACTAGT | 1 cut(s) | 599 |
| Sse9I | AATT | 6 cut(s) | 36, 638, 1183, 1235, 1515, 1525 |
| SseBI | AGGCCT | 3 cut(s) | 126, 419, 867 |
| SspDI | GGCGCC | 1 cut(s) | 686 |
| SspMI | CTAG | 9 cut(s) | 122, 369, 581, 600, 764, 884, 1046, 1104, 1500 |
| StuI | AGGCCT | 3 cut(s) | 126, 419, 867 |
| StyI | CCWWGG | 4 cut(s) | 121, 368, 868, 1460 |
| TaaI | ACNGT | 2 cut(s) | 410, 711 |
| TaiI | ACGT | 2 cut(s) | 630, 1684 |
| TaqI | TCGA | 6 cut(s) | 129, 329, 521, 612, 930, 1320 |
| TasI | AATT | 6 cut(s) | 36, 638, 1183, 1235, 1515, 1525 |
| TatI | WGTACW | 3 cut(s) | 768, 1048, 1651 |
| TauI | GCSGC | 4 cut(s) | 684, 687, 1140, 1499 |
| TfiI | GAWTC | 3 cut(s) | 654, 670, 1698 |
| Tru1I | TTAA | 5 cut(s) | 35, 204, 248, 1506, 1512 |
| Tru9I | TTAA | 5 cut(s) | 35, 204, 248, 1506, 1512 |
| TscAI | CASTG | 3 cut(s) | 397, 820, 1020 |
| TseI | GCWGC | 4 cut(s) | 45, 358, 1169, 1534 |
| TspDTI | ATGAA | 5 cut(s) | 222, 258, 1203, 1560, 1605 |
| TspGWI | ACGGA | 1 cut(s) | 1040 |
| TspMI | CCCGGG | 1 cut(s) | 1409 |
| TspRI | CASTG | 3 cut(s) | 397, 820, 1020 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1401, 1466 |
| VpaK11BI | GGWCC | 3 cut(s) | 459, 818, 1042 |
| VspI | ATTAAT | 1 cut(s) | 204 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 882 |
| XapI | RAATTY | 1 cut(s) | 1515 |
| XbaI | TCTAGA | 1 cut(s) | 763 |
| XceI | RCATGY | 2 cut(s) | 1652, 1658 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 866 |
| XhoI | CTCGAG | 1 cut(s) | 128 |
| XmaI | CCCGGG | 1 cut(s) | 1409 |
| XmaJI | CCTAGG | 2 cut(s) | 121, 368 |
| XmiI | GTMKAC | 3 cut(s) | 509, 521, 604 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1063 |
| XspI | CTAG | 9 cut(s) | 122, 369, 581, 600, 764, 884, 1046, 1104, 1500 |
| ZrmI | AGTACT | 2 cut(s) | 770, 1050 |
| Zsp2I | ATGCAT | 1 cut(s) | 924 |