No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1345 |
| Acc36I | ACCTGC | 2 cut(s) | 546, 1345 |
| AccB1I | GGYRCC | 2 cut(s) | 564, 2234 |
| AccBSI | CCGCTC | 1 cut(s) | 2089 |
| AccI | GTMKAC | 2 cut(s) | 208, 1565 |
| AccIII | TCCGGA | 1 cut(s) | 1762 |
| AclWI | GGATC | 6 cut(s) | 311, 696, 709, 1102, 1603, 2289 |
| AcoI | YGGCCR | 2 cut(s) | 389, 519 |
| AcsI | RAATTY | 1 cut(s) | 1368 |
| AcuI | CTGAAG | 5 cut(s) | 1446, 1764, 1875, 2303, 2324 |
| AcyI | GRCGYC | 1 cut(s) | 2180 |
| AdeI | CACNNNGTG | 1 cut(s) | 620 |
| AfaI | GTAC | 4 cut(s) | 648, 1253, 2210, 2220 |
| AgsI | TTSAA | 9 cut(s) | 259, 295, 529, 721, 1241, 1249, 1361, 2299, 2333 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 93, 125, 1140, 1172, 1941, 1973 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2496, 2528 |
| Alw21I | GWGCWC | 4 cut(s) | 275, 892, 1224, 2106 |
| Alw26I | GTCTC | 7 cut(s) | 98, 670, 843, 1185, 1192, 2162, 2430 |
| Alw44I | GTGCAC | 1 cut(s) | 888 |
| AlwI | GGATC | 6 cut(s) | 311, 696, 709, 1102, 1603, 2289 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1652, 2018 |
| Aor13HI | TCCGGA | 1 cut(s) | 1762 |
| ApaI | GGGCCC | 2 cut(s) | 161, 2168 |
| ApaLI | GTGCAC | 1 cut(s) | 888 |
| ApeKI | GCWGC | 5 cut(s) | 906, 1472, 1787, 1943, 2408 |
| ApoI | RAATTY | 1 cut(s) | 1368 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1208, 1240 |
| AspA2I | CCTAGG | 1 cut(s) | 2288 |
| AspLEI | GCGC | 7 cut(s) | 614, 1285, 1287, 1324, 1342, 1491, 1943 |
| AsuC2I | CCSGG | 6 cut(s) | 249, 265, 769, 1003, 1396, 2184 |
| AsuHPI | GGTGA | 8 cut(s) | 13, 267, 468, 474, 1142, 1625, 1790, 2306 |
| AsuNHI | GCTAGC | 2 cut(s) | 983, 2065 |
| AvaII | GGWCC | 3 cut(s) | 243, 868, 1076 |
| AvrII | CCTAGG | 1 cut(s) | 2288 |
| AxyI | CCTNAGG | 1 cut(s) | 1293 |
| BaeGI | GKGCMC | 4 cut(s) | 161, 569, 892, 2168 |
| BalI | TGGCCA | 1 cut(s) | 521 |
| BamHI | GGATCC | 1 cut(s) | 701 |
| BanI | GGYRCC | 2 cut(s) | 564, 2234 |
| BanII | GRGCYC | 6 cut(s) | 161, 176, 275, 1224, 1348, 2168 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1241, 1273 |
| BbsI | GAAGAC | 3 cut(s) | 1440, 1458, 1752 |
| Bbv12I | GWGCWC | 4 cut(s) | 275, 892, 1224, 2106 |
| BbvCI | CCTCAGC | 1 cut(s) | 2404 |
| BbvI | GCAGC | 5 cut(s) | 893, 1459, 1799, 1955, 2395 |
| BccI | CCATC | 8 cut(s) | 32, 349, 444, 487, 539, 583, 1119, 2246 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 411, 445 |
| BcnI | CCSGG | 6 cut(s) | 249, 265, 769, 1003, 1396, 2184 |
| BcoDI | GTCTC | 7 cut(s) | 98, 670, 843, 1185, 1192, 2162, 2430 |
| BfmI | CTRYAG | 2 cut(s) | 1410, 1566 |
| BfuAI | ACCTGC | 2 cut(s) | 546, 1345 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2471 |
| BglII | AGATCT | 1 cut(s) | 2335 |
| BisI | GCNGC | 9 cut(s) | 220, 323, 392, 907, 939, 1473, 1788, 1944, 2409 |
| BlnI | CCTAGG | 1 cut(s) | 2288 |
| BlsI | GCNGC | 9 cut(s) | 221, 324, 393, 908, 940, 1474, 1789, 1945, 2410 |
| BmcAI | AGTACT | 2 cut(s) | 648, 2220 |
| Bme18I | GGWCC | 3 cut(s) | 243, 868, 1076 |
| BmsI | GCATC | 8 cut(s) | 87, 178, 464, 618, 653, 675, 1628, 2125 |
| BmtI | GCTAGC | 2 cut(s) | 987, 2069 |
| BpiI | GAAGAC | 3 cut(s) | 1440, 1458, 1752 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 950, 982 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2404 |
| BpuEI | CTTGAG | 4 cut(s) | 1167, 1469, 1561, 1833 |
| BpuMI | CCSGG | 6 cut(s) | 249, 265, 769, 1003, 1396, 2184 |
| Bsa29I | ATCGAT | 1 cut(s) | 344 |
| BsaBI | GATNNNNATC | 2 cut(s) | 454, 721 |
| BsaHI | GRCGYC | 1 cut(s) | 2180 |
| BsaI | GGTCTC | 1 cut(s) | 1185 |
| BsaWI | WCCGGW | 3 cut(s) | 149, 624, 1762 |
| Bse1I | ACTGG | 8 cut(s) | 230, 654, 684, 1078, 1355, 1554, 1612, 2491 |
| Bse21I | CCTNAGG | 1 cut(s) | 1293 |
| Bse8I | GATNNNNATC | 2 cut(s) | 454, 721 |
| BseAI | TCCGGA | 1 cut(s) | 1762 |
| BseCI | ATCGAT | 1 cut(s) | 344 |
| BseGI | GGATG | 6 cut(s) | 598, 633, 666, 1303, 1619, 2506 |
| BseJI | GATNNNNATC | 2 cut(s) | 454, 721 |
| BseMII | CTCAG | 5 cut(s) | 864, 1006, 1284, 1728, 2418 |
| BseNI | ACTGG | 8 cut(s) | 230, 654, 684, 1078, 1355, 1554, 1612, 2491 |
| BsePI | GCGCGC | 1 cut(s) | 1283 |
| BseRI | GAGGAG | 1 cut(s) | 1749 |
| BseSI | GKGCMC | 4 cut(s) | 161, 569, 892, 2168 |
| BseXI | GCAGC | 5 cut(s) | 893, 1459, 1799, 1955, 2395 |
| BsgI | GTGCAG | 1 cut(s) | 1733 |
| Bsh1285I | CGRYCG | 2 cut(s) | 886, 1539 |
| BshNI | GGYRCC | 2 cut(s) | 564, 2234 |
| BshVI | ATCGAT | 1 cut(s) | 344 |
| BsiEI | CGRYCG | 2 cut(s) | 886, 1539 |
| BsiHKAI | GWGCWC | 4 cut(s) | 275, 892, 1224, 2106 |
| BsiSI | CCGG | 9 cut(s) | 150, 249, 265, 625, 769, 1003, 1395, 1763, 2184 |
| BslFI | GGGAC | 3 cut(s) | 881, 1175, 2308 |
| BsmAI | GTCTC | 7 cut(s) | 98, 670, 843, 1185, 1192, 2162, 2430 |
| BsmBI | CGTCTC | 2 cut(s) | 843, 2162 |
| BsmFI | GGGAC | 3 cut(s) | 881, 1175, 2308 |
| BsmI | GAATGC | 1 cut(s) | 1758 |
| Bso31I | GGTCTC | 1 cut(s) | 1185 |
| Bsp120I | GGGCCC | 2 cut(s) | 157, 2164 |
| Bsp1286I | GDGCHC | 9 cut(s) | 161, 176, 275, 569, 892, 1224, 1348, 2106, 2168 |
| Bsp13I | TCCGGA | 1 cut(s) | 1762 |
| Bsp68I | TCGCGA | 2 cut(s) | 803, 1229 |
| BspCNI | CTCAG | 5 cut(s) | 865, 1005, 1285, 1729, 2417 |
| BspDI | ATCGAT | 1 cut(s) | 344 |
| BspEI | TCCGGA | 1 cut(s) | 1762 |
| BspMI | ACCTGC | 2 cut(s) | 546, 1345 |
| BspOI | GCTAGC | 2 cut(s) | 987, 2069 |
| BspPI | GGATC | 6 cut(s) | 311, 696, 709, 1102, 1603, 2289 |
| BspQI | GCTCTTC | 1 cut(s) | 1229 |
| BspT107I | GGYRCC | 2 cut(s) | 564, 2234 |
| BspTNI | GGTCTC | 1 cut(s) | 1185 |
| BsrBI | CCGCTC | 1 cut(s) | 2089 |
| BsrI | ACTGG | 8 cut(s) | 230, 654, 684, 1078, 1355, 1554, 1612, 2491 |
| BssHII | GCGCGC | 1 cut(s) | 1283 |
| BssNI | GRCGYC | 1 cut(s) | 2180 |
| BssT1I | CCWWGG | 1 cut(s) | 2288 |
| Bst4CI | ACNGT | 3 cut(s) | 109, 1730, 2359 |
| Bst6I | CTCTTC | 3 cut(s) | 30, 1229, 1444 |
| BstACI | GRCGYC | 1 cut(s) | 2180 |
| BstDEI | CTNAG | 6 cut(s) | 873, 992, 1293, 1737, 1965, 2404 |
| BstDSI | CCRYGG | 2 cut(s) | 167, 2446 |
| BstF5I | GGATG | 6 cut(s) | 598, 633, 666, 1303, 1619, 2506 |
| BstHHI | GCGC | 7 cut(s) | 614, 1285, 1287, 1324, 1342, 1491, 1943 |
| BstMAI | GTCTC | 7 cut(s) | 98, 670, 843, 1185, 1192, 2162, 2430 |
| BstMCI | CGRYCG | 2 cut(s) | 886, 1539 |
| BstNSI | RCATGY | 1 cut(s) | 1099 |
| BstSFI | CTRYAG | 2 cut(s) | 1410, 1566 |
| BstSLI | GKGCMC | 4 cut(s) | 161, 569, 892, 2168 |
| BstV1I | GCAGC | 5 cut(s) | 893, 1459, 1799, 1955, 2395 |
| BstV2I | GAAGAC | 3 cut(s) | 1440, 1458, 1752 |
| BstX2I | RGATCY | 4 cut(s) | 303, 701, 2281, 2335 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2447 |
| BstYI | RGATCY | 4 cut(s) | 303, 701, 2281, 2335 |
| Bsu15I | ATCGAT | 1 cut(s) | 344 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1293 |
| BsuTUI | ATCGAT | 1 cut(s) | 344 |
| BtgI | CCRYGG | 2 cut(s) | 167, 2446 |
| BtsCI | GGATG | 6 cut(s) | 598, 633, 666, 1303, 1619, 2506 |
| BtsI | GCAGTG | 2 cut(s) | 757, 1467 |
| BtsIMutI | CAGTG | 5 cut(s) | 757, 1467, 1605, 2241, 2484 |
| BtuMI | TCGCGA | 2 cut(s) | 803, 1229 |
| BveI | ACCTGC | 2 cut(s) | 546, 1345 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1652, 2018 |
| CfoI | GCGC | 7 cut(s) | 614, 1285, 1287, 1324, 1342, 1491, 1943 |
| ClaI | ATCGAT | 1 cut(s) | 344 |
| CseI | GACGC | 2 cut(s) | 1037, 2188 |
| Csp6I | GTAC | 4 cut(s) | 647, 1252, 2209, 2219 |
| CviAII | CATG | 6 cut(s) | 517, 632, 851, 1096, 1377, 2378 |
| CviQI | GTAC | 4 cut(s) | 647, 1252, 2209, 2219 |
| DdeI | CTNAG | 6 cut(s) | 873, 992, 1293, 1737, 1965, 2404 |
| DraIII | CACNNNGTG | 1 cut(s) | 620 |
| EaeI | YGGCCR | 2 cut(s) | 389, 519 |
| Eam1104I | CTCTTC | 3 cut(s) | 30, 1229, 1444 |
| EarI | CTCTTC | 3 cut(s) | 30, 1229, 1444 |
| EciI | GGCGGA | 4 cut(s) | 283, 730, 1069, 1320 |
| Ecl136II | GAGCTC | 2 cut(s) | 273, 1222 |
| Eco130I | CCWWGG | 1 cut(s) | 2288 |
| Eco147I | AGGCCT | 3 cut(s) | 118, 2148, 2474 |
| Eco24I | GRGCYC | 6 cut(s) | 161, 176, 275, 1224, 1348, 2168 |
| Eco31I | GGTCTC | 1 cut(s) | 1185 |
| Eco47I | GGWCC | 3 cut(s) | 243, 868, 1076 |
| Eco53kI | GAGCTC | 2 cut(s) | 273, 1222 |
| Eco57I | CTGAAG | 5 cut(s) | 1446, 1764, 1875, 2303, 2324 |
| Eco81I | CCTNAGG | 1 cut(s) | 1293 |
| EcoICRI | GAGCTC | 2 cut(s) | 273, 1222 |
| EcoO109I | RGGNCCY | 6 cut(s) | 158, 243, 868, 1571, 1814, 2165 |
| EcoT14I | CCWWGG | 1 cut(s) | 2288 |
| EcoT22I | ATGCAT | 2 cut(s) | 633, 668 |
| EcoT38I | GRGCYC | 6 cut(s) | 161, 176, 275, 1224, 1348, 2168 |
| ErhI | CCWWGG | 1 cut(s) | 2288 |
| Esp3I | CGTCTC | 2 cut(s) | 843, 2162 |
| FaeI | CATG | 6 cut(s) | 520, 635, 854, 1099, 1380, 2381 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 521, 553 |
| FaqI | GGGAC | 3 cut(s) | 881, 1175, 2308 |
| FatI | CATG | 6 cut(s) | 516, 631, 850, 1095, 1376, 2377 |
| FauI | CCCGC | 4 cut(s) | 318, 1910, 2200, 2521 |
| FblI | GTMKAC | 2 cut(s) | 208, 1565 |
| Fnu4HI | GCNGC | 9 cut(s) | 220, 323, 392, 907, 939, 1473, 1788, 1944, 2409 |
| FokI | GGATG | 6 cut(s) | 605, 640, 653, 1310, 1606, 2513 |
| FriOI | GRGCYC | 6 cut(s) | 161, 176, 275, 1224, 1348, 2168 |
| Fsp4HI | GCNGC | 9 cut(s) | 220, 323, 392, 907, 939, 1473, 1788, 1944, 2409 |
| GlaI | GCGC | 7 cut(s) | 613, 1284, 1286, 1323, 1341, 1490, 1942 |
| GluI | GCNGC | 9 cut(s) | 220, 323, 392, 907, 939, 1473, 1788, 1944, 2409 |
| HapII | CCGG | 9 cut(s) | 150, 249, 265, 625, 769, 1003, 1395, 1763, 2184 |
| HgaI | GACGC | 2 cut(s) | 1037, 2188 |
| HhaI | GCGC | 7 cut(s) | 614, 1285, 1287, 1324, 1342, 1491, 1943 |
| Hin1I | GRCGYC | 1 cut(s) | 2180 |
| Hin1II | CATG | 6 cut(s) | 520, 635, 854, 1099, 1380, 2381 |
| Hin6I | GCGC | 7 cut(s) | 612, 1283, 1285, 1322, 1340, 1489, 1941 |
| HinP1I | GCGC | 7 cut(s) | 612, 1283, 1285, 1322, 1340, 1489, 1941 |
| HincII | GTYRAC | 1 cut(s) | 1195 |
| HindII | GTYRAC | 1 cut(s) | 1195 |
| HindIII | AAGCTT | 1 cut(s) | 2300 |
| HpaI | GTTAAC | 1 cut(s) | 1195 |
| HpaII | CCGG | 9 cut(s) | 150, 249, 265, 625, 769, 1003, 1395, 1763, 2184 |
| HphI | GGTGA | 8 cut(s) | 13, 267, 468, 474, 1142, 1625, 1790, 2306 |
| Hpy166II | GTNNAC | 5 cut(s) | 209, 890, 1195, 1566, 1781 |
| Hpy8I | GTNNAC | 5 cut(s) | 209, 890, 1195, 1566, 1781 |
| Hpy99I | CGWCG | 2 cut(s) | 1148, 1220 |
| HpyAV | CCTTC | 9 cut(s) | 234, 438, 681, 1142, 1399, 1586, 2082, 2138, 2327 |
| HpyCH4III | ACNGT | 3 cut(s) | 109, 1730, 2359 |
| HpyCH4IV | ACGT | 3 cut(s) | 460, 512, 619 |
| HpyCH4V | TGCA | 7 cut(s) | 516, 542, 631, 666, 890, 1708, 1714 |
| HpyF3I | CTNAG | 6 cut(s) | 873, 992, 1293, 1737, 1965, 2404 |
| HpySE526I | ACGT | 3 cut(s) | 460, 512, 619 |
| Hsp92I | GRCGYC | 1 cut(s) | 2180 |
| Hsp92II | CATG | 6 cut(s) | 520, 635, 854, 1099, 1380, 2381 |
| HspAI | GCGC | 7 cut(s) | 612, 1283, 1285, 1322, 1340, 1489, 1941 |
| KflI | GGGWCCC | 1 cut(s) | 868 |
| Kpn2I | TCCGGA | 1 cut(s) | 1762 |
| KspAI | GTTAAC | 1 cut(s) | 1195 |
| LguI | GCTCTTC | 1 cut(s) | 1229 |
| LmnI | GCTCC | 5 cut(s) | 179, 270, 1333, 1502, 1876 |
| Lsp1109I | GCAGC | 5 cut(s) | 893, 1459, 1799, 1955, 2395 |
| LweI | GCATC | 8 cut(s) | 87, 178, 464, 618, 653, 675, 1628, 2125 |
| MaeII | ACGT | 3 cut(s) | 460, 512, 619 |
| MaeIII | GTNAC | 3 cut(s) | 620, 1025, 2110 |
| MbiI | CCGCTC | 1 cut(s) | 2089 |
| MfeI | CAATTG | 2 cut(s) | 494, 2390 |
| MflI | RGATCY | 4 cut(s) | 303, 701, 2281, 2335 |
| MhlI | GDGCHC | 9 cut(s) | 161, 176, 275, 569, 892, 1224, 1348, 2106, 2168 |
| MlsI | TGGCCA | 1 cut(s) | 521 |
| MluCI | AATT | 9 cut(s) | 494, 523, 1134, 1368, 1875, 1911, 2252, 2294, 2390 |
| MluNI | TGGCCA | 1 cut(s) | 521 |
| MlyI | GAGTC | 3 cut(s) | 1161, 1430, 1643 |
| MmeI | TCCRAC | 5 cut(s) | 295, 381, 1524, 1626, 2482 |
| Mox20I | TGGCCA | 1 cut(s) | 521 |
| Mph1103I | ATGCAT | 2 cut(s) | 633, 668 |
| MroI | TCCGGA | 1 cut(s) | 1762 |
| MscI | TGGCCA | 1 cut(s) | 521 |
| MseI | TTAA | 4 cut(s) | 789, 1194, 1914, 2519 |
| Msp20I | TGGCCA | 1 cut(s) | 521 |
| MspA1I | CMGCKG | 2 cut(s) | 1696, 2408 |
| MspI | CCGG | 9 cut(s) | 150, 249, 265, 625, 769, 1003, 1395, 1763, 2184 |
| MunI | CAATTG | 2 cut(s) | 494, 2390 |
| Mva1269I | GAATGC | 1 cut(s) | 1758 |
| NciI | CCSGG | 6 cut(s) | 249, 265, 769, 1003, 1396, 2184 |
| NheI | GCTAGC | 2 cut(s) | 983, 2065 |
| NlaIII | CATG | 6 cut(s) | 520, 635, 854, 1099, 1380, 2381 |
| NmeAIII | GCCGAG | 1 cut(s) | 769 |
| NmuCI | GTSAC | 3 cut(s) | 620, 1025, 2110 |
| NruI | TCGCGA | 2 cut(s) | 803, 1229 |
| NsiI | ATGCAT | 2 cut(s) | 633, 668 |
| NspI | RCATGY | 1 cut(s) | 1099 |
| PaeI | GCATGC | 1 cut(s) | 1099 |
| PaqCI | CACCTGC | 1 cut(s) | 1345 |
| PasI | CCCWGGG | 2 cut(s) | 1518, 1994 |
| PauI | GCGCGC | 1 cut(s) | 1283 |
| PceI | AGGCCT | 3 cut(s) | 118, 2148, 2474 |
| PciSI | GCTCTTC | 1 cut(s) | 1229 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1152 |
| PctI | GAATGC | 1 cut(s) | 1758 |
| PfeI | GAWTC | 9 cut(s) | 396, 532, 717, 1399, 1688, 1717, 1820, 1853, 2329 |
| PfoI | TCCNGGA | 1 cut(s) | 1800 |
| PkrI | GCNGC | 9 cut(s) | 221, 324, 393, 908, 940, 1474, 1789, 1945, 2410 |
| Ple19I | CGATCG | 2 cut(s) | 886, 1539 |
| PleI | GAGTC | 3 cut(s) | 1160, 1430, 1643 |
| PpsI | GAGTC | 3 cut(s) | 1160, 1430, 1643 |
| PpuMI | RGGWCCY | 2 cut(s) | 243, 868 |
| Psp124BI | GAGCTC | 2 cut(s) | 275, 1224 |
| Psp5II | RGGWCCY | 2 cut(s) | 243, 868 |
| PspOMI | GGGCCC | 2 cut(s) | 157, 2164 |
| PspPPI | RGGWCCY | 2 cut(s) | 243, 868 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1652, 2018 |
| PsuI | RGATCY | 4 cut(s) | 303, 701, 2281, 2335 |
| PteI | GCGCGC | 1 cut(s) | 1283 |
| PvuI | CGATCG | 2 cut(s) | 886, 1539 |
| PvuII | CAGCTG | 2 cut(s) | 1696, 2408 |
| RruI | TCGCGA | 2 cut(s) | 803, 1229 |
| RsaI | GTAC | 4 cut(s) | 648, 1253, 2210, 2220 |
| RsaNI | GTAC | 4 cut(s) | 647, 1252, 2209, 2219 |
| SacI | GAGCTC | 2 cut(s) | 275, 1224 |
| SapI | GCTCTTC | 1 cut(s) | 1229 |
| SaqAI | TTAA | 4 cut(s) | 789, 1194, 1914, 2519 |
| SatI | GCNGC | 9 cut(s) | 220, 323, 392, 907, 939, 1473, 1788, 1944, 2409 |
| ScaI | AGTACT | 2 cut(s) | 648, 2220 |
| SchI | GAGTC | 3 cut(s) | 1161, 1430, 1643 |
| SduI | GDGCHC | 9 cut(s) | 161, 176, 275, 569, 892, 1224, 1348, 2106, 2168 |
| SfaNI | GCATC | 8 cut(s) | 87, 178, 464, 618, 653, 675, 1628, 2125 |
| SfcI | CTRYAG | 2 cut(s) | 1410, 1566 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 2471 |
| SinI | GGWCC | 3 cut(s) | 243, 868, 1076 |
| SmlI | CTYRAG | 4 cut(s) | 1182, 1484, 1576, 1848 |
| SmoI | CTYRAG | 4 cut(s) | 1182, 1484, 1576, 1848 |
| SphI | GCATGC | 1 cut(s) | 1099 |
| Sse9I | AATT | 9 cut(s) | 494, 523, 1134, 1368, 1875, 1911, 2252, 2294, 2390 |
| SseBI | AGGCCT | 3 cut(s) | 118, 2148, 2474 |
| SstI | GAGCTC | 2 cut(s) | 275, 1224 |
| StuI | AGGCCT | 3 cut(s) | 118, 2148, 2474 |
| StyI | CCWWGG | 1 cut(s) | 2288 |
| TaaI | ACNGT | 3 cut(s) | 109, 1730, 2359 |
| TaiI | ACGT | 3 cut(s) | 463, 515, 622 |
| TasI | AATT | 9 cut(s) | 494, 523, 1134, 1368, 1875, 1911, 2252, 2294, 2390 |
| TatI | WGTACW | 2 cut(s) | 646, 2218 |
| TauI | GCSGC | 4 cut(s) | 222, 325, 394, 941 |
| TfiI | GAWTC | 9 cut(s) | 396, 532, 717, 1399, 1688, 1717, 1820, 1853, 2329 |
| Tru1I | TTAA | 4 cut(s) | 789, 1194, 1914, 2519 |
| Tru9I | TTAA | 4 cut(s) | 789, 1194, 1914, 2519 |
| TscAI | CASTG | 5 cut(s) | 764, 1474, 1612, 2248, 2491 |
| TseFI | GTSAC | 3 cut(s) | 620, 1025, 2110 |
| TseI | GCWGC | 5 cut(s) | 906, 1472, 1787, 1943, 2408 |
| Tsp45I | GTSAC | 3 cut(s) | 620, 1025, 2110 |
| TspDTI | ATGAA | 5 cut(s) | 1287, 1464, 1545, 1547, 2517 |
| TspGWI | ACGGA | 2 cut(s) | 353, 2504 |
| TspRI | CASTG | 5 cut(s) | 764, 1474, 1612, 2248, 2491 |
| VneI | GTGCAC | 1 cut(s) | 888 |
| VpaK11BI | GGWCC | 3 cut(s) | 243, 868, 1076 |
| XapI | RAATTY | 1 cut(s) | 1368 |
| XbaI | TCTAGA | 2 cut(s) | 641, 1720 |
| XceI | RCATGY | 1 cut(s) | 1099 |
| XmaJI | CCTAGG | 1 cut(s) | 2288 |
| XmiI | GTMKAC | 2 cut(s) | 208, 1565 |
| ZrmI | AGTACT | 2 cut(s) | 648, 2220 |
| Zsp2I | ATGCAT | 2 cut(s) | 633, 668 |