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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 744 |
| Acc65I | GGTACC | 1 cut(s) | 1457 |
| AccB1I | GGYRCC | 1 cut(s) | 1457 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 59 |
| AccBSI | CCGCTC | 1 cut(s) | 1325 |
| AccII | CGCG | 7 cut(s) | 36, 38, 453, 819, 1077, 1136, 1437 |
| AclWI | GGATC | 5 cut(s) | 280, 395, 604, 938, 1156 |
| AcoI | YGGCCR | 1 cut(s) | 28 |
| AcsI | RAATTY | 1 cut(s) | 957 |
| AcuI | CTGAAG | 4 cut(s) | 120, 546, 585, 953 |
| AcyI | GRCGYC | 1 cut(s) | 1200 |
| AfaI | GTAC | 3 cut(s) | 660, 693, 1459 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 26, 37, 59, 139, 289, 415, 619 |
| AflIII | ACRYGT | 1 cut(s) | 1134 |
| AgeI | ACCGGT | 1 cut(s) | 595 |
| AgsI | TTSAA | 9 cut(s) | 159, 698, 962, 1115, 1143, 1174, 1215, 1235, 1271 |
| AjiI | CACGTC | 1 cut(s) | 532 |
| AjnI | CCWGG | 8 cut(s) | 6, 381, 496, 728, 772, 853, 1032, 1062 |
| AluBI | AGCT | 7 cut(s) | 185, 632, 979, 1073, 1368, 1416, 1486 |
| AluI | AGCT | 7 cut(s) | 185, 632, 979, 1073, 1368, 1416, 1486 |
| Alw26I | GTCTC | 3 cut(s) | 894, 915, 1413 |
| AlwI | GGATC | 5 cut(s) | 280, 395, 604, 938, 1156 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 59, 524, 635 |
| Ama87I | CYCGRG | 2 cut(s) | 1001, 1301 |
| AoxI | GGCC | 8 cut(s) | 28, 213, 279, 332, 420, 850, 972, 1038 |
| ApeKI | GCWGC | 4 cut(s) | 557, 632, 635, 638 |
| ApoI | RAATTY | 1 cut(s) | 957 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1363, 1395 |
| AsiGI | ACCGGT | 1 cut(s) | 595 |
| Asp718I | GGTACC | 1 cut(s) | 1457 |
| AspLEI | GCGC | 5 cut(s) | 38, 223, 397, 779, 1439 |
| AspS9I | GGNCC | 6 cut(s) | 214, 280, 421, 851, 951, 973 |
| AsuC2I | CCSGG | 1 cut(s) | 290 |
| AsuHPI | GGTGA | 6 cut(s) | 306, 595, 734, 935, 1093, 1432 |
| AvaI | CYCGRG | 2 cut(s) | 1001, 1301 |
| AvaII | GGWCC | 1 cut(s) | 951 |
| BalI | TGGCCA | 1 cut(s) | 30 |
| BanI | GGYRCC | 1 cut(s) | 1457 |
| BanII | GRGCYC | 1 cut(s) | 793 |
| BauI | CACGAG | 2 cut(s) | 687, 885 |
| BbsI | GAAGAC | 3 cut(s) | 534, 1122, 1331 |
| BbvCI | CCTCAGC | 1 cut(s) | 181 |
| BbvI | GCAGC | 4 cut(s) | 544, 619, 622, 625 |
| BccI | CCATC | 2 cut(s) | 488, 836 |
| BceAI | ACGGC | 2 cut(s) | 1213, 1390 |
| BciT130I | CCWGG | 8 cut(s) | 8, 383, 498, 730, 774, 855, 1034, 1064 |
| BcnI | CCSGG | 1 cut(s) | 290 |
| BcoDI | GTCTC | 3 cut(s) | 894, 915, 1413 |
| BfaI | CTAG | 2 cut(s) | 669, 1166 |
| BfmI | CTRYAG | 2 cut(s) | 465, 1333 |
| BfoI | RGCGCY | 2 cut(s) | 224, 398 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 278 |
| BglII | AGATCT | 3 cut(s) | 152, 964, 1329 |
| BisI | GCNGC | 8 cut(s) | 20, 57, 335, 558, 633, 636, 639, 1038 |
| BlsI | GCNGC | 8 cut(s) | 21, 58, 336, 559, 634, 637, 640, 1039 |
| Bme1390I | CCNGG | 9 cut(s) | 8, 290, 383, 498, 730, 774, 855, 1034, 1064 |
| Bme18I | GGWCC | 1 cut(s) | 951 |
| BmeT110I | CYCGRG | 2 cut(s) | 1001, 1301 |
| BmgBI | CACGTC | 1 cut(s) | 532 |
| BmgT120I | GGNCC | 6 cut(s) | 214, 280, 421, 851, 951, 973 |
| BmiI | GGNNCC | 8 cut(s) | 96, 112, 178, 232, 624, 953, 1014, 1459 |
| BmrFI | CCNGG | 9 cut(s) | 8, 290, 383, 498, 730, 774, 855, 1034, 1064 |
| BmrI | ACTGGG | 2 cut(s) | 177, 512 |
| BmsI | GCATC | 1 cut(s) | 788 |
| BmuI | ACTGGG | 2 cut(s) | 177, 512 |
| BpiI | GAAGAC | 3 cut(s) | 534, 1122, 1331 |
| BpmI | CTGGAG | 3 cut(s) | 612, 815, 1043 |
| Bpu10I | CCTNAGC | 2 cut(s) | 181, 263 |
| BpuEI | CTTGAG | 1 cut(s) | 1053 |
| BpuMI | CCSGG | 1 cut(s) | 290 |
| BsaHI | GRCGYC | 1 cut(s) | 1200 |
| BsaJI | CCNNGG | 4 cut(s) | 7, 729, 853, 1032 |
| BsaWI | WCCGGW | 2 cut(s) | 595, 948 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1304, 1334 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 26, 37, 59, 139, 289, 415, 619 |
| Bse118I | RCCGGY | 1 cut(s) | 595 |
| Bse1I | ACTGG | 5 cut(s) | 172, 232, 282, 518, 687 |
| Bse3DI | GCAATG | 1 cut(s) | 274 |
| BseBI | CCWGG | 8 cut(s) | 8, 383, 498, 730, 774, 855, 1034, 1064 |
| BseDI | CCNNGG | 4 cut(s) | 7, 729, 853, 1032 |
| BseGI | GGATG | 1 cut(s) | 779 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 26, 37, 59, 139, 289, 415, 619 |
| BseMI | GCAATG | 1 cut(s) | 274 |
| BseMII | CTCAG | 5 cut(s) | 195, 254, 825, 1340, 1378 |
| BseNI | ACTGG | 5 cut(s) | 172, 232, 282, 518, 687 |
| BseRI | GAGGAG | 3 cut(s) | 169, 696, 1309 |
| BseXI | GCAGC | 4 cut(s) | 544, 619, 622, 625 |
| BseYI | CCCAGC | 2 cut(s) | 52, 975 |
| Bsh1236I | CGCG | 7 cut(s) | 36, 38, 453, 819, 1077, 1136, 1437 |
| BshFI | GGCC | 8 cut(s) | 30, 215, 281, 334, 422, 852, 974, 1040 |
| BshNI | GGYRCC | 1 cut(s) | 1457 |
| BshTI | ACCGGT | 1 cut(s) | 595 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1001, 1301 |
| BsiSI | CCGG | 4 cut(s) | 290, 461, 596, 949 |
| BslFI | GGGAC | 6 cut(s) | 536, 741, 746, 937, 1329, 1337 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 26, 37, 59, 139, 289, 415, 619 |
| BsmAI | GTCTC | 3 cut(s) | 894, 915, 1413 |
| BsmBI | CGTCTC | 2 cut(s) | 894, 1413 |
| BsmFI | GGGAC | 6 cut(s) | 536, 741, 746, 937, 1329, 1337 |
| BsmI | GAATGC | 1 cut(s) | 1169 |
| BsnI | GGCC | 8 cut(s) | 30, 215, 281, 334, 422, 852, 974, 1040 |
| BsoBI | CYCGRG | 2 cut(s) | 1001, 1301 |
| Bsp1286I | GDGCHC | 1 cut(s) | 793 |
| Bsp143I | GATC | 9 cut(s) | 152, 285, 400, 609, 930, 964, 1161, 1329, 1360 |
| Bsp68I | TCGCGA | 2 cut(s) | 819, 1077 |
| BspANI | GGCC | 8 cut(s) | 30, 215, 281, 334, 422, 852, 974, 1040 |
| BspCNI | CTCAG | 5 cut(s) | 194, 255, 826, 1339, 1377 |
| BspFNI | CGCG | 7 cut(s) | 36, 38, 453, 819, 1077, 1136, 1437 |
| BspLI | GGNNCC | 8 cut(s) | 96, 112, 178, 232, 624, 953, 1014, 1459 |
| BspMAI | CTGCAG | 1 cut(s) | 1337 |
| BspPI | GGATC | 5 cut(s) | 280, 395, 604, 938, 1156 |
| BspQI | GCTCTTC | 2 cut(s) | 354, 486 |
| BspT107I | GGYRCC | 1 cut(s) | 1457 |
| BsrBI | CCGCTC | 1 cut(s) | 1325 |
| BsrDI | GCAATG | 1 cut(s) | 274 |
| BsrFI | RCCGGY | 1 cut(s) | 595 |
| BsrI | ACTGG | 5 cut(s) | 172, 232, 282, 518, 687 |
| BssAI | RCCGGY | 1 cut(s) | 595 |
| BssECI | CCNNGG | 4 cut(s) | 7, 729, 853, 1032 |
| BssMI | GATC | 9 cut(s) | 152, 285, 400, 609, 930, 964, 1161, 1329, 1360 |
| BssNI | GRCGYC | 1 cut(s) | 1200 |
| BssSI | CACGAG | 2 cut(s) | 687, 885 |
| Bst2BI | CACGAG | 2 cut(s) | 687, 885 |
| Bst2UI | CCWGG | 8 cut(s) | 8, 383, 498, 730, 774, 855, 1034, 1064 |
| Bst4CI | ACNGT | 6 cut(s) | 66, 85, 142, 311, 746, 1351 |
| Bst6I | CTCTTC | 7 cut(s) | 121, 354, 486, 573, 1281, 1286, 1476 |
| BstACI | GRCGYC | 1 cut(s) | 1200 |
| BstC8I | GCNNGC | 5 cut(s) | 455, 821, 825, 997, 1075 |
| BstDEI | CTNAG | 7 cut(s) | 181, 263, 834, 895, 1326, 1364, 1480 |
| BstF5I | GGATG | 1 cut(s) | 779 |
| BstFNI | CGCG | 7 cut(s) | 36, 38, 453, 819, 1077, 1136, 1437 |
| BstH2I | RGCGCY | 2 cut(s) | 224, 398 |
| BstHHI | GCGC | 5 cut(s) | 38, 223, 397, 779, 1439 |
| BstKTI | GATC | 9 cut(s) | 155, 288, 403, 612, 933, 967, 1164, 1332, 1363 |
| BstMAI | GTCTC | 3 cut(s) | 894, 915, 1413 |
| BstMBI | GATC | 9 cut(s) | 152, 285, 400, 609, 930, 964, 1161, 1329, 1360 |
| BstNI | CCWGG | 8 cut(s) | 8, 383, 498, 730, 774, 855, 1034, 1064 |
| BstNSI | RCATGY | 1 cut(s) | 659 |
| BstSCI | CCNGG | 9 cut(s) | 6, 288, 381, 496, 728, 772, 853, 1032, 1062 |
| BstSFI | CTRYAG | 2 cut(s) | 465, 1333 |
| BstUI | CGCG | 7 cut(s) | 36, 38, 453, 819, 1077, 1136, 1437 |
| BstV1I | GCAGC | 4 cut(s) | 544, 619, 622, 625 |
| BstV2I | GAAGAC | 3 cut(s) | 534, 1122, 1331 |
| BstX2I | RGATCY | 5 cut(s) | 152, 285, 964, 1161, 1329 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 487 |
| BstYI | RGATCY | 5 cut(s) | 152, 285, 964, 1161, 1329 |
| BsuRI | GGCC | 8 cut(s) | 30, 215, 281, 334, 422, 852, 974, 1040 |
| BtgZI | GCGATG | 1 cut(s) | 466 |
| BtrI | CACGTC | 1 cut(s) | 532 |
| BtsCI | GGATG | 1 cut(s) | 779 |
| BtsIMutI | CAGTG | 3 cut(s) | 90, 196, 831 |
| BtuMI | TCGCGA | 2 cut(s) | 819, 1077 |
| Cac8I | GCNNGC | 5 cut(s) | 455, 821, 825, 997, 1075 |
| CaiI | CAGNNNCTG | 3 cut(s) | 59, 524, 635 |
| CfoI | GCGC | 5 cut(s) | 38, 223, 397, 779, 1439 |
| Cfr10I | RCCGGY | 1 cut(s) | 595 |
| Cfr13I | GGNCC | 6 cut(s) | 214, 280, 421, 851, 951, 973 |
| CseI | GACGC | 2 cut(s) | 356, 1189 |
| Csp6I | GTAC | 3 cut(s) | 659, 692, 1458 |
| CspAI | ACCGGT | 1 cut(s) | 595 |
| CviAII | CATG | 4 cut(s) | 656, 988, 1007, 1375 |
| CviQI | GTAC | 3 cut(s) | 659, 692, 1458 |
| DdeI | CTNAG | 7 cut(s) | 181, 263, 834, 895, 1326, 1364, 1480 |
| DpnI | GATC | 9 cut(s) | 154, 287, 402, 611, 932, 966, 1163, 1331, 1362 |
| DpnII | GATC | 9 cut(s) | 152, 285, 400, 609, 930, 964, 1161, 1329, 1360 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 744 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 744 |
| EaeI | YGGCCR | 1 cut(s) | 28 |
| Eam1104I | CTCTTC | 7 cut(s) | 121, 354, 486, 573, 1281, 1286, 1476 |
| EarI | CTCTTC | 7 cut(s) | 121, 354, 486, 573, 1281, 1286, 1476 |
| Eco24I | GRGCYC | 1 cut(s) | 793 |
| Eco47I | GGWCC | 1 cut(s) | 951 |
| Eco57I | CTGAAG | 4 cut(s) | 120, 546, 585, 953 |
| Eco88I | CYCGRG | 2 cut(s) | 1001, 1301 |
| EcoRII | CCWGG | 8 cut(s) | 6, 381, 496, 728, 772, 853, 1032, 1062 |
| EcoT22I | ATGCAT | 1 cut(s) | 657 |
| EcoT38I | GRGCYC | 1 cut(s) | 793 |
| Esp3I | CGTCTC | 2 cut(s) | 894, 1413 |
| FaeI | CATG | 4 cut(s) | 659, 991, 1010, 1378 |
| FaqI | GGGAC | 6 cut(s) | 536, 741, 746, 937, 1329, 1337 |
| FatI | CATG | 4 cut(s) | 655, 987, 1006, 1374 |
| FauI | CCCGC | 4 cut(s) | 141, 517, 1004, 1132 |
| Fnu4HI | GCNGC | 8 cut(s) | 20, 57, 335, 558, 633, 636, 639, 1038 |
| FokI | GGATG | 1 cut(s) | 766 |
| FriOI | GRGCYC | 1 cut(s) | 793 |
| Fsp4HI | GCNGC | 8 cut(s) | 20, 57, 335, 558, 633, 636, 639, 1038 |
| FspBI | CTAG | 2 cut(s) | 669, 1166 |
| GlaI | GCGC | 5 cut(s) | 37, 222, 396, 778, 1438 |
| GluI | GCNGC | 8 cut(s) | 20, 57, 335, 558, 633, 636, 639, 1038 |
| GsaI | CCCAGC | 2 cut(s) | 56, 979 |
| GsuI | CTGGAG | 3 cut(s) | 612, 815, 1043 |
| HaeII | RGCGCY | 2 cut(s) | 224, 398 |
| HaeIII | GGCC | 8 cut(s) | 30, 215, 281, 334, 422, 852, 974, 1040 |
| HapII | CCGG | 4 cut(s) | 290, 461, 596, 949 |
| HgaI | GACGC | 2 cut(s) | 356, 1189 |
| HhaI | GCGC | 5 cut(s) | 38, 223, 397, 779, 1439 |
| Hin1I | GRCGYC | 1 cut(s) | 1200 |
| Hin1II | CATG | 4 cut(s) | 659, 991, 1010, 1378 |
| Hin6I | GCGC | 5 cut(s) | 36, 221, 395, 777, 1437 |
| HinP1I | GCGC | 5 cut(s) | 36, 221, 395, 777, 1437 |
| HincII | GTYRAC | 1 cut(s) | 1249 |
| HindII | GTYRAC | 1 cut(s) | 1249 |
| HinfI | GANTC | 4 cut(s) | 545, 754, 878, 1447 |
| HpaII | CCGG | 4 cut(s) | 290, 461, 596, 949 |
| HphI | GGTGA | 6 cut(s) | 306, 595, 734, 935, 1093, 1432 |
| Hpy166II | GTNNAC | 4 cut(s) | 314, 586, 692, 1249 |
| Hpy188I | TCNGA | 9 cut(s) | 121, 346, 565, 1045, 1159, 1286, 1329, 1446, 1452 |
| Hpy188III | TCNNGA | 9 cut(s) | 159, 669, 710, 794, 818, 887, 962, 1076, 1090 |
| Hpy8I | GTNNAC | 4 cut(s) | 314, 586, 692, 1249 |
| Hpy99I | CGWCG | 4 cut(s) | 125, 593, 819, 1205 |
| HpyAV | CCTTC | 5 cut(s) | 33, 101, 167, 188, 1159 |
| HpyCH4III | ACNGT | 6 cut(s) | 66, 85, 142, 311, 746, 1351 |
| HpyCH4IV | ACGT | 2 cut(s) | 531, 588 |
| HpyCH4V | TGCA | 5 cut(s) | 4, 295, 655, 1243, 1335 |
| HpyF3I | CTNAG | 7 cut(s) | 181, 263, 834, 895, 1326, 1364, 1480 |
| HpySE526I | ACGT | 2 cut(s) | 531, 588 |
| Hsp92I | GRCGYC | 1 cut(s) | 1200 |
| Hsp92II | CATG | 4 cut(s) | 659, 991, 1010, 1378 |
| HspAI | GCGC | 5 cut(s) | 36, 221, 395, 777, 1437 |
| KpnI | GGTACC | 1 cut(s) | 1461 |
| Kzo9I | GATC | 9 cut(s) | 152, 285, 400, 609, 930, 964, 1161, 1329, 1360 |
| LguI | GCTCTTC | 2 cut(s) | 354, 486 |
| LmnI | GCTCC | 3 cut(s) | 182, 622, 1024 |
| Lsp1109I | GCAGC | 4 cut(s) | 544, 619, 622, 625 |
| LweI | GCATC | 1 cut(s) | 788 |
| MaeI | CTAG | 2 cut(s) | 669, 1166 |
| MaeII | ACGT | 2 cut(s) | 531, 588 |
| MaeIII | GTNAC | 1 cut(s) | 700 |
| MalI | GATC | 9 cut(s) | 154, 287, 402, 611, 932, 966, 1163, 1331, 1362 |
| MbiI | CCGCTC | 1 cut(s) | 1325 |
| MboI | GATC | 9 cut(s) | 152, 285, 400, 609, 930, 964, 1161, 1329, 1360 |
| MflI | RGATCY | 5 cut(s) | 152, 285, 964, 1161, 1329 |
| MhlI | GDGCHC | 1 cut(s) | 793 |
| MlsI | TGGCCA | 1 cut(s) | 30 |
| MluCI | AATT | 3 cut(s) | 957, 1143, 1174 |
| MluI | ACGCGT | 1 cut(s) | 1134 |
| MluNI | TGGCCA | 1 cut(s) | 30 |
| MlyI | GAGTC | 2 cut(s) | 554, 763 |
| MmeI | TCCRAC | 1 cut(s) | 144 |
| Mox20I | TGGCCA | 1 cut(s) | 30 |
| Mph1103I | ATGCAT | 1 cut(s) | 657 |
| MscI | TGGCCA | 1 cut(s) | 30 |
| MseI | TTAA | 1 cut(s) | 537 |
| MslI | CAYNNNNRTG | 2 cut(s) | 485, 784 |
| Msp20I | TGGCCA | 1 cut(s) | 30 |
| MspA1I | CMGCKG | 4 cut(s) | 59, 185, 632, 979 |
| MspI | CCGG | 4 cut(s) | 290, 461, 596, 949 |
| MspR9I | CCNGG | 9 cut(s) | 8, 290, 383, 498, 730, 774, 855, 1034, 1064 |
| Mva1269I | GAATGC | 1 cut(s) | 1169 |
| MvaI | CCWGG | 8 cut(s) | 8, 383, 498, 730, 774, 855, 1034, 1064 |
| MvnI | CGCG | 7 cut(s) | 36, 38, 453, 819, 1077, 1136, 1437 |
| NciI | CCSGG | 1 cut(s) | 290 |
| NdeII | GATC | 9 cut(s) | 152, 285, 400, 609, 930, 964, 1161, 1329, 1360 |
| NlaIII | CATG | 4 cut(s) | 659, 991, 1010, 1378 |
| NlaIV | GGNNCC | 8 cut(s) | 96, 112, 178, 232, 624, 953, 1014, 1459 |
| NruI | TCGCGA | 2 cut(s) | 819, 1077 |
| NsiI | ATGCAT | 1 cut(s) | 657 |
| NspI | RCATGY | 1 cut(s) | 659 |
| PaeR7I | CTCGAG | 1 cut(s) | 1001 |
| PciSI | GCTCTTC | 2 cut(s) | 354, 486 |
| PctI | GAATGC | 1 cut(s) | 1169 |
| PfeI | GAWTC | 2 cut(s) | 878, 1447 |
| PflFI | GACNNNGTC | 1 cut(s) | 1199 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 59 |
| PinAI | ACCGGT | 1 cut(s) | 595 |
| PkrI | GCNGC | 8 cut(s) | 21, 58, 336, 559, 634, 637, 640, 1039 |
| PleI | GAGTC | 2 cut(s) | 553, 762 |
| PpsI | GAGTC | 2 cut(s) | 553, 762 |
| Psp6I | CCWGG | 8 cut(s) | 6, 381, 496, 728, 772, 853, 1032, 1062 |
| PspFI | CCCAGC | 2 cut(s) | 52, 975 |
| PspGI | CCWGG | 8 cut(s) | 6, 381, 496, 728, 772, 853, 1032, 1062 |
| PspN4I | GGNNCC | 8 cut(s) | 96, 112, 178, 232, 624, 953, 1014, 1459 |
| PspPI | GGNCC | 6 cut(s) | 214, 280, 421, 851, 951, 973 |
| PspXI | VCTCGAGB | 1 cut(s) | 1001 |
| PstI | CTGCAG | 1 cut(s) | 1337 |
| PstNI | CAGNNNCTG | 3 cut(s) | 59, 524, 635 |
| PsuI | RGATCY | 5 cut(s) | 152, 285, 964, 1161, 1329 |
| PsyI | GACNNNGTC | 1 cut(s) | 1199 |
| PvuII | CAGCTG | 3 cut(s) | 185, 632, 979 |
| RruI | TCGCGA | 2 cut(s) | 819, 1077 |
| RsaI | GTAC | 3 cut(s) | 660, 693, 1459 |
| RsaNI | GTAC | 3 cut(s) | 659, 692, 1458 |
| RseI | CAYNNNNRTG | 2 cut(s) | 485, 784 |
| SapI | GCTCTTC | 2 cut(s) | 354, 486 |
| SaqAI | TTAA | 1 cut(s) | 537 |
| SatI | GCNGC | 8 cut(s) | 20, 57, 335, 558, 633, 636, 639, 1038 |
| Sau3AI | GATC | 9 cut(s) | 152, 285, 400, 609, 930, 964, 1161, 1329, 1360 |
| Sau96I | GGNCC | 6 cut(s) | 214, 280, 421, 851, 951, 973 |
| SchI | GAGTC | 2 cut(s) | 554, 763 |
| ScrFI | CCNGG | 9 cut(s) | 8, 290, 383, 498, 730, 774, 855, 1034, 1064 |
| SduI | GDGCHC | 1 cut(s) | 793 |
| SfaNI | GCATC | 1 cut(s) | 788 |
| SfcI | CTRYAG | 2 cut(s) | 465, 1333 |
| Sfr274I | CTCGAG | 1 cut(s) | 1001 |
| SinI | GGWCC | 1 cut(s) | 951 |
| SlaI | CTCGAG | 1 cut(s) | 1001 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 485, 784 |
| SmlI | CTYRAG | 2 cut(s) | 1001, 1068 |
| SmoI | CTYRAG | 2 cut(s) | 1001, 1068 |
| Sse9I | AATT | 3 cut(s) | 957, 1143, 1174 |
| SspMI | CTAG | 2 cut(s) | 669, 1166 |
| StyD4I | CCNGG | 9 cut(s) | 6, 288, 381, 496, 728, 772, 853, 1032, 1062 |
| TaaI | ACNGT | 6 cut(s) | 66, 85, 142, 311, 746, 1351 |
| TaiI | ACGT | 2 cut(s) | 534, 591 |
| TaqI | TCGA | 6 cut(s) | 591, 1002, 1091, 1203, 1359, 1370 |
| TasI | AATT | 3 cut(s) | 957, 1143, 1174 |
| TatI | WGTACW | 1 cut(s) | 691 |
| TauI | GCSGC | 4 cut(s) | 22, 59, 337, 1040 |
| TfiI | GAWTC | 2 cut(s) | 878, 1447 |
| Tru1I | TTAA | 1 cut(s) | 537 |
| Tru9I | TTAA | 1 cut(s) | 537 |
| TscAI | CASTG | 3 cut(s) | 90, 196, 838 |
| TseI | GCWGC | 4 cut(s) | 557, 632, 635, 638 |
| TspDTI | ATGAA | 2 cut(s) | 134, 1141 |
| TspGWI | ACGGA | 3 cut(s) | 107, 750, 772 |
| TspRI | CASTG | 3 cut(s) | 90, 196, 838 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1199 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 59 |
| VpaK11BI | GGWCC | 1 cut(s) | 951 |
| XapI | RAATTY | 1 cut(s) | 957 |
| XbaI | TCTAGA | 1 cut(s) | 668 |
| XceI | RCATGY | 1 cut(s) | 659 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 38, 224 |
| XhoI | CTCGAG | 1 cut(s) | 1001 |
| XspI | CTAG | 2 cut(s) | 669, 1166 |
| Zsp2I | ATGCAT | 1 cut(s) | 657 |