Ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 308 |
| AccB1I | GGYRCC | 1 cut(s) | 751 |
| AccI | GTMKAC | 1 cut(s) | 528 |
| AccII | CGCG | 7 cut(s) | 33, 106, 279, 286, 292, 360, 866 |
| AccIII | TCCGGA | 1 cut(s) | 808 |
| AclWI | GGATC | 3 cut(s) | 831, 844, 971 |
| AcoI | YGGCCR | 3 cut(s) | 239, 717, 890 |
| AcsI | RAATTY | 1 cut(s) | 659 |
| AcuI | CTGAAG | 1 cut(s) | 412 |
| AcyI | GRCGYC | 1 cut(s) | 752 |
| AfaI | GTAC | 3 cut(s) | 140, 772, 1101 |
| AfeI | AGCGCT | 1 cut(s) | 565 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 231, 362, 491, 748 |
| AflIII | ACRYGT | 1 cut(s) | 31 |
| AgeI | ACCGGT | 1 cut(s) | 741 |
| AgsI | TTSAA | 2 cut(s) | 14, 644 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 742 |
| AluBI | AGCT | 2 cut(s) | 1044, 1051 |
| AluI | AGCT | 2 cut(s) | 1044, 1051 |
| Alw21I | GWGCWC | 2 cut(s) | 259, 1053 |
| Alw26I | GTCTC | 4 cut(s) | 32, 183, 257, 1009 |
| Alw44I | GTGCAC | 1 cut(s) | 255 |
| AlwI | GGATC | 3 cut(s) | 831, 844, 971 |
| Aor13HI | TCCGGA | 1 cut(s) | 808 |
| Aor51HI | AGCGCT | 1 cut(s) | 565 |
| AoxI | GGCC | 8 cut(s) | 94, 158, 239, 301, 478, 717, 890, 1084 |
| ApaLI | GTGCAC | 1 cut(s) | 255 |
| ApeKI | GCWGC | 3 cut(s) | 314, 383, 561 |
| ApoI | RAATTY | 1 cut(s) | 659 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 416, 448 |
| AsiGI | ACCGGT | 1 cut(s) | 741 |
| AspLEI | GCGC | 4 cut(s) | 286, 292, 566, 754 |
| AspS9I | GGNCC | 4 cut(s) | 95, 159, 702, 811 |
| AsuC2I | CCSGG | 3 cut(s) | 88, 186, 608 |
| AsuHPI | GGTGA | 2 cut(s) | 41, 316 |
| AvaII | GGWCC | 2 cut(s) | 702, 811 |
| BaeGI | GKGCMC | 1 cut(s) | 259 |
| BalI | TGGCCA | 1 cut(s) | 241 |
| BamHI | GGATCC | 1 cut(s) | 836 |
| BanI | GGYRCC | 1 cut(s) | 751 |
| BanII | GRGCYC | 2 cut(s) | 734, 1053 |
| BbsI | GAAGAC | 1 cut(s) | 137 |
| Bbv12I | GWGCWC | 2 cut(s) | 259, 1053 |
| BbvI | GCAGC | 3 cut(s) | 301, 370, 573 |
| BceAI | ACGGC | 5 cut(s) | 173, 484, 732, 981, 986 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 980, 1014 |
| BcnI | CCSGG | 3 cut(s) | 88, 186, 608 |
| BcoDI | GTCTC | 4 cut(s) | 32, 183, 257, 1009 |
| BfaI | CTAG | 2 cut(s) | 167, 299 |
| BfmI | CTRYAG | 1 cut(s) | 315 |
| BfoI | RGCGCY | 2 cut(s) | 567, 755 |
| BfuAI | ACCTGC | 1 cut(s) | 308 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1083 |
| BglII | AGATCT | 1 cut(s) | 295 |
| BisI | GCNGC | 7 cut(s) | 199, 315, 384, 562, 720, 864, 1029 |
| BlpI | GCTNAGC | 1 cut(s) | 549 |
| BlsI | GCNGC | 7 cut(s) | 200, 316, 385, 563, 721, 865, 1030 |
| Bme1390I | CCNGG | 3 cut(s) | 88, 186, 608 |
| Bme18I | GGWCC | 2 cut(s) | 702, 811 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 742 |
| BmgT120I | GGNCC | 4 cut(s) | 95, 159, 702, 811 |
| BmiI | GGNNCC | 3 cut(s) | 753, 813, 838 |
| BmrFI | CCNGG | 3 cut(s) | 88, 186, 608 |
| BmsI | GCATC | 3 cut(s) | 115, 773, 795 |
| BpiI | GAAGAC | 1 cut(s) | 137 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 436, 468 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 549 |
| BpuMI | CCSGG | 3 cut(s) | 88, 186, 608 |
| Bsa29I | ATCGAT | 1 cut(s) | 489 |
| BsaHI | GRCGYC | 1 cut(s) | 752 |
| BsaI | GGTCTC | 1 cut(s) | 32 |
| BsaJI | CCNNGG | 2 cut(s) | 543, 1032 |
| BsaWI | WCCGGW | 3 cut(s) | 623, 741, 808 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 45, 75 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 231, 362, 491, 748 |
| Bse118I | RCCGGY | 2 cut(s) | 741, 888 |
| Bse1I | ACTGG | 3 cut(s) | 311, 481, 697 |
| BseAI | TCCGGA | 1 cut(s) | 808 |
| BseCI | ATCGAT | 1 cut(s) | 489 |
| BseDI | CCNNGG | 2 cut(s) | 543, 1032 |
| BseGI | GGATG | 4 cut(s) | 242, 462, 786, 1017 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 231, 362, 491, 748 |
| BseMII | CTCAG | 2 cut(s) | 563, 1054 |
| BseNI | ACTGG | 3 cut(s) | 311, 481, 697 |
| BseSI | GKGCMC | 1 cut(s) | 259 |
| BseX3I | CGGCCG | 2 cut(s) | 717, 890 |
| BseXI | GCAGC | 3 cut(s) | 301, 370, 573 |
| BseYI | CCCAGC | 2 cut(s) | 97, 201 |
| BsgI | GTGCAG | 1 cut(s) | 655 |
| Bsh1236I | CGCG | 7 cut(s) | 33, 106, 279, 286, 292, 360, 866 |
| Bsh1285I | CGRYCG | 4 cut(s) | 624, 720, 893, 994 |
| BshFI | GGCC | 8 cut(s) | 96, 160, 241, 303, 480, 719, 892, 1086 |
| BshNI | GGYRCC | 1 cut(s) | 751 |
| BshTI | ACCGGT | 1 cut(s) | 741 |
| BshVI | ATCGAT | 1 cut(s) | 489 |
| BsiEI | CGRYCG | 4 cut(s) | 624, 720, 893, 994 |
| BsiHKAI | GWGCWC | 2 cut(s) | 259, 1053 |
| BsiSI | CCGG | 7 cut(s) | 87, 186, 607, 624, 742, 809, 889 |
| BslFI | GGGAC | 1 cut(s) | 32 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 231, 362, 491, 748 |
| BsmAI | GTCTC | 4 cut(s) | 32, 183, 257, 1009 |
| BsmBI | CGTCTC | 1 cut(s) | 1009 |
| BsmFI | GGGAC | 1 cut(s) | 32 |
| BsmI | GAATGC | 1 cut(s) | 316 |
| BsnI | GGCC | 8 cut(s) | 96, 160, 241, 303, 480, 719, 892, 1086 |
| Bso31I | GGTCTC | 1 cut(s) | 32 |
| Bsp1286I | GDGCHC | 3 cut(s) | 259, 734, 1053 |
| Bsp13I | TCCGGA | 1 cut(s) | 808 |
| Bsp143I | GATC | 6 cut(s) | 295, 337, 797, 836, 894, 976 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 549 |
| Bsp19I | CCATGG | 1 cut(s) | 543 |
| Bsp68I | TCGCGA | 1 cut(s) | 279 |
| BspANI | GGCC | 8 cut(s) | 96, 160, 241, 303, 480, 719, 892, 1086 |
| BspCNI | CTCAG | 2 cut(s) | 562, 1053 |
| BspDI | ATCGAT | 1 cut(s) | 489 |
| BspEI | TCCGGA | 1 cut(s) | 808 |
| BspFNI | CGCG | 7 cut(s) | 33, 106, 279, 286, 292, 360, 866 |
| BspLI | GGNNCC | 3 cut(s) | 753, 813, 838 |
| BspMAI | CTGCAG | 1 cut(s) | 319 |
| BspMI | ACCTGC | 1 cut(s) | 308 |
| BspPI | GGATC | 3 cut(s) | 831, 844, 971 |
| BspT107I | GGYRCC | 1 cut(s) | 751 |
| BspTNI | GGTCTC | 1 cut(s) | 32 |
| BsrFI | RCCGGY | 2 cut(s) | 741, 888 |
| BsrI | ACTGG | 3 cut(s) | 311, 481, 697 |
| BssAI | RCCGGY | 2 cut(s) | 741, 888 |
| BssECI | CCNNGG | 2 cut(s) | 543, 1032 |
| BssMI | GATC | 6 cut(s) | 295, 337, 797, 836, 894, 976 |
| BssNI | GRCGYC | 1 cut(s) | 752 |
| BssT1I | CCWWGG | 1 cut(s) | 543 |
| Bst4CI | ACNGT | 2 cut(s) | 143, 917 |
| BstACI | GRCGYC | 1 cut(s) | 752 |
| BstC8I | GCNNGC | 7 cut(s) | 288, 374, 497, 594, 686, 890, 1075 |
| BstDEI | CTNAG | 3 cut(s) | 22, 549, 1040 |
| BstDSI | CCRYGG | 1 cut(s) | 543 |
| BstF5I | GGATG | 4 cut(s) | 242, 462, 786, 1017 |
| BstFNI | CGCG | 7 cut(s) | 33, 106, 279, 286, 292, 360, 866 |
| BstH2I | RGCGCY | 2 cut(s) | 567, 755 |
| BstHHI | GCGC | 4 cut(s) | 286, 292, 566, 754 |
| BstKTI | GATC | 6 cut(s) | 298, 340, 800, 839, 897, 979 |
| BstMAI | GTCTC | 4 cut(s) | 32, 183, 257, 1009 |
| BstMBI | GATC | 6 cut(s) | 295, 337, 797, 836, 894, 976 |
| BstMCI | CGRYCG | 4 cut(s) | 624, 720, 893, 994 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 505, 719, 885, 1083 |
| BstNSI | RCATGY | 2 cut(s) | 768, 910 |
| BstSCI | CCNGG | 3 cut(s) | 86, 184, 606 |
| BstSFI | CTRYAG | 1 cut(s) | 315 |
| BstSLI | GKGCMC | 1 cut(s) | 259 |
| BstUI | CGCG | 7 cut(s) | 33, 106, 279, 286, 292, 360, 866 |
| BstV1I | GCAGC | 3 cut(s) | 301, 370, 573 |
| BstV2I | GAAGAC | 1 cut(s) | 137 |
| BstX2I | RGATCY | 2 cut(s) | 295, 836 |
| BstYI | RGATCY | 2 cut(s) | 295, 836 |
| BstZI | CGGCCG | 2 cut(s) | 717, 890 |
| Bsu15I | ATCGAT | 1 cut(s) | 489 |
| BsuRI | GGCC | 8 cut(s) | 96, 160, 241, 303, 480, 719, 892, 1086 |
| BsuTUI | ATCGAT | 1 cut(s) | 489 |
| BtgI | CCRYGG | 1 cut(s) | 543 |
| BtgZI | GCGATG | 1 cut(s) | 115 |
| BtsCI | GGATG | 4 cut(s) | 242, 462, 786, 1017 |
| BtsIMutI | CAGTG | 3 cut(s) | 304, 474, 690 |
| BtuMI | TCGCGA | 1 cut(s) | 279 |
| BveI | ACCTGC | 1 cut(s) | 308 |
| Cac8I | GCNNGC | 7 cut(s) | 288, 374, 497, 594, 686, 890, 1075 |
| CfoI | GCGC | 4 cut(s) | 286, 292, 566, 754 |
| Cfr10I | RCCGGY | 2 cut(s) | 741, 888 |
| Cfr13I | GGNCC | 4 cut(s) | 95, 159, 702, 811 |
| ClaI | ATCGAT | 1 cut(s) | 489 |
| CseI | GACGC | 1 cut(s) | 39 |
| Csp6I | GTAC | 3 cut(s) | 139, 771, 1100 |
| CspAI | ACCGGT | 1 cut(s) | 741 |
| CviAII | CATG | 6 cut(s) | 136, 509, 544, 699, 765, 907 |
| CviQI | GTAC | 3 cut(s) | 139, 771, 1100 |
| DdeI | CTNAG | 3 cut(s) | 22, 549, 1040 |
| DinI | GGCGCC | 1 cut(s) | 753 |
| DpnI | GATC | 6 cut(s) | 297, 339, 799, 838, 896, 978 |
| DpnII | GATC | 6 cut(s) | 295, 337, 797, 836, 894, 976 |
| DriI | GACNNNNNGTC | 1 cut(s) | 742 |
| EaeI | YGGCCR | 3 cut(s) | 239, 717, 890 |
| EagI | CGGCCG | 2 cut(s) | 717, 890 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 742 |
| EciI | GGCGGA | 1 cut(s) | 850 |
| Ecl136II | GAGCTC | 1 cut(s) | 1051 |
| EclXI | CGGCCG | 2 cut(s) | 717, 890 |
| Eco130I | CCWWGG | 1 cut(s) | 543 |
| Eco24I | GRGCYC | 2 cut(s) | 734, 1053 |
| Eco31I | GGTCTC | 1 cut(s) | 32 |
| Eco32I | GATATC | 1 cut(s) | 873 |
| Eco47I | GGWCC | 2 cut(s) | 702, 811 |
| Eco47III | AGCGCT | 1 cut(s) | 565 |
| Eco52I | CGGCCG | 2 cut(s) | 717, 890 |
| Eco53kI | GAGCTC | 1 cut(s) | 1051 |
| Eco57I | CTGAAG | 1 cut(s) | 412 |
| EcoICRI | GAGCTC | 1 cut(s) | 1051 |
| EcoRV | GATATC | 1 cut(s) | 873 |
| EcoT14I | CCWWGG | 1 cut(s) | 543 |
| EcoT22I | ATGCAT | 1 cut(s) | 788 |
| EcoT38I | GRGCYC | 2 cut(s) | 734, 1053 |
| EgeI | GGCGCC | 1 cut(s) | 753 |
| EheI | GGCGCC | 1 cut(s) | 753 |
| ErhI | CCWWGG | 1 cut(s) | 543 |
| Esp3I | CGTCTC | 1 cut(s) | 1009 |
| FaeI | CATG | 6 cut(s) | 139, 512, 547, 702, 768, 910 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 928, 960 |
| FaqI | GGGAC | 1 cut(s) | 32 |
| FatI | CATG | 6 cut(s) | 135, 508, 543, 698, 764, 906 |
| FauI | CCCGC | 4 cut(s) | 279, 884, 893, 1082 |
| FblI | GTMKAC | 1 cut(s) | 528 |
| Fnu4HI | GCNGC | 7 cut(s) | 199, 315, 384, 562, 720, 864, 1029 |
| FokI | GGATG | 4 cut(s) | 229, 449, 773, 1004 |
| FriOI | GRGCYC | 2 cut(s) | 734, 1053 |
| Fsp4HI | GCNGC | 7 cut(s) | 199, 315, 384, 562, 720, 864, 1029 |
| FspBI | CTAG | 2 cut(s) | 167, 299 |
| GlaI | GCGC | 4 cut(s) | 285, 291, 565, 753 |
| GluI | GCNGC | 7 cut(s) | 199, 315, 384, 562, 720, 864, 1029 |
| GsaI | CCCAGC | 2 cut(s) | 101, 205 |
| HaeII | RGCGCY | 2 cut(s) | 567, 755 |
| HaeIII | GGCC | 8 cut(s) | 96, 160, 241, 303, 480, 719, 892, 1086 |
| HapII | CCGG | 7 cut(s) | 87, 186, 607, 624, 742, 809, 889 |
| HgaI | GACGC | 1 cut(s) | 39 |
| HhaI | GCGC | 4 cut(s) | 286, 292, 566, 754 |
| Hin1I | GRCGYC | 1 cut(s) | 752 |
| Hin1II | CATG | 6 cut(s) | 139, 512, 547, 702, 768, 910 |
| Hin6I | GCGC | 4 cut(s) | 284, 290, 564, 752 |
| HinP1I | GCGC | 4 cut(s) | 284, 290, 564, 752 |
| HincII | GTYRAC | 1 cut(s) | 529 |
| HindII | GTYRAC | 1 cut(s) | 529 |
| HinfI | GANTC | 3 cut(s) | 181, 521, 939 |
| HpaII | CCGG | 7 cut(s) | 87, 186, 607, 624, 742, 809, 889 |
| HphI | GGTGA | 2 cut(s) | 41, 316 |
| Hpy166II | GTNNAC | 2 cut(s) | 257, 529 |
| Hpy188I | TCNGA | 6 cut(s) | 76, 155, 176, 431, 736, 1015 |
| Hpy188III | TCNNGA | 8 cut(s) | 278, 400, 809, 819, 834, 840, 989, 1021 |
| Hpy8I | GTNNAC | 2 cut(s) | 257, 529 |
| Hpy99I | CGWCG | 3 cut(s) | 159, 783, 977 |
| HpyAV | CCTTC | 7 cut(s) | 8, 74, 171, 491, 646, 825, 954 |
| HpyCH4III | ACNGT | 2 cut(s) | 143, 917 |
| HpyCH4V | TGCA | 7 cut(s) | 4, 257, 317, 508, 672, 684, 786 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 505, 719, 885, 1083 |
| HpyF3I | CTNAG | 3 cut(s) | 22, 549, 1040 |
| Hsp92I | GRCGYC | 1 cut(s) | 752 |
| Hsp92II | CATG | 6 cut(s) | 139, 512, 547, 702, 768, 910 |
| HspAI | GCGC | 4 cut(s) | 284, 290, 564, 752 |
| KasI | GGCGCC | 1 cut(s) | 751 |
| Kpn2I | TCCGGA | 1 cut(s) | 808 |
| KroI | GCCGGC | 1 cut(s) | 888 |
| KroNI | GCCGGC | 1 cut(s) | 890 |
| Kzo9I | GATC | 6 cut(s) | 295, 337, 797, 836, 894, 976 |
| LmnI | GCTCC | 1 cut(s) | 1063 |
| Lsp1109I | GCAGC | 3 cut(s) | 301, 370, 573 |
| LweI | GCATC | 3 cut(s) | 115, 773, 795 |
| MaeI | CTAG | 2 cut(s) | 167, 299 |
| MalI | GATC | 6 cut(s) | 297, 339, 799, 838, 896, 978 |
| MboI | GATC | 6 cut(s) | 295, 337, 797, 836, 894, 976 |
| MboII | GAAGA | 6 cut(s) | 137, 344, 849, 880, 945, 948 |
| MfeI | CAATTG | 1 cut(s) | 1094 |
| MflI | RGATCY | 2 cut(s) | 295, 836 |
| MhlI | GDGCHC | 3 cut(s) | 259, 734, 1053 |
| MlsI | TGGCCA | 1 cut(s) | 241 |
| MluCI | AATT | 8 cut(s) | 235, 515, 630, 659, 898, 924, 1079, 1094 |
| MluI | ACGCGT | 1 cut(s) | 31 |
| MluNI | TGGCCA | 1 cut(s) | 241 |
| Mly113I | GGCGCC | 1 cut(s) | 752 |
| MlyI | GAGTC | 2 cut(s) | 175, 515 |
| MmeI | TCCRAC | 3 cut(s) | 99, 178, 438 |
| Mox20I | TGGCCA | 1 cut(s) | 241 |
| Mph1103I | ATGCAT | 1 cut(s) | 788 |
| MroI | TCCGGA | 1 cut(s) | 808 |
| MroNI | GCCGGC | 1 cut(s) | 888 |
| MscI | TGGCCA | 1 cut(s) | 241 |
| MseI | TTAA | 1 cut(s) | 923 |
| MslI | CAYNNNNRTG | 2 cut(s) | 134, 309 |
| Msp20I | TGGCCA | 1 cut(s) | 241 |
| MspI | CCGG | 7 cut(s) | 87, 186, 607, 624, 742, 809, 889 |
| MspR9I | CCNGG | 3 cut(s) | 88, 186, 608 |
| MunI | CAATTG | 1 cut(s) | 1094 |
| Mva1269I | GAATGC | 1 cut(s) | 316 |
| MvnI | CGCG | 7 cut(s) | 33, 106, 279, 286, 292, 360, 866 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 505, 719, 885, 1083 |
| NaeI | GCCGGC | 1 cut(s) | 890 |
| NarI | GGCGCC | 1 cut(s) | 752 |
| NciI | CCSGG | 3 cut(s) | 88, 186, 608 |
| NcoI | CCATGG | 1 cut(s) | 543 |
| NdeII | GATC | 6 cut(s) | 295, 337, 797, 836, 894, 976 |
| NgoMIV | GCCGGC | 1 cut(s) | 888 |
| NlaIII | CATG | 6 cut(s) | 139, 512, 547, 702, 768, 910 |
| NlaIV | GGNNCC | 3 cut(s) | 753, 813, 838 |
| NmeAIII | GCCGAG | 1 cut(s) | 1057 |
| NruI | TCGCGA | 1 cut(s) | 279 |
| NsiI | ATGCAT | 1 cut(s) | 788 |
| NspI | RCATGY | 2 cut(s) | 768, 910 |
| PctI | GAATGC | 1 cut(s) | 316 |
| PdiI | GCCGGC | 1 cut(s) | 890 |
| PfeI | GAWTC | 1 cut(s) | 939 |
| PflFI | GACNNNGTC | 1 cut(s) | 524 |
| PfoI | TCCNGGA | 1 cut(s) | 184 |
| PinAI | ACCGGT | 1 cut(s) | 741 |
| PkrI | GCNGC | 7 cut(s) | 200, 316, 385, 563, 721, 865, 1030 |
| PleI | GAGTC | 2 cut(s) | 175, 515 |
| PluTI | GGCGCC | 1 cut(s) | 755 |
| PpsI | GAGTC | 2 cut(s) | 175, 515 |
| Psp124BI | GAGCTC | 1 cut(s) | 1053 |
| PspFI | CCCAGC | 2 cut(s) | 97, 201 |
| PspN4I | GGNNCC | 3 cut(s) | 753, 813, 838 |
| PspPI | GGNCC | 4 cut(s) | 95, 159, 702, 811 |
| PstI | CTGCAG | 1 cut(s) | 319 |
| PsuI | RGATCY | 2 cut(s) | 295, 836 |
| PsyI | GACNNNGTC | 1 cut(s) | 524 |
| RruI | TCGCGA | 1 cut(s) | 279 |
| RsaI | GTAC | 3 cut(s) | 140, 772, 1101 |
| RsaNI | GTAC | 3 cut(s) | 139, 771, 1100 |
| RseI | CAYNNNNRTG | 2 cut(s) | 134, 309 |
| SacI | GAGCTC | 1 cut(s) | 1053 |
| SalI | GTCGAC | 1 cut(s) | 527 |
| SaqAI | TTAA | 1 cut(s) | 923 |
| SatI | GCNGC | 7 cut(s) | 199, 315, 384, 562, 720, 864, 1029 |
| Sau3AI | GATC | 6 cut(s) | 295, 337, 797, 836, 894, 976 |
| Sau96I | GGNCC | 4 cut(s) | 95, 159, 702, 811 |
| SchI | GAGTC | 2 cut(s) | 175, 515 |
| ScrFI | CCNGG | 3 cut(s) | 88, 186, 608 |
| SduI | GDGCHC | 3 cut(s) | 259, 734, 1053 |
| SetI | ASST | 7 cut(s) | 112, 322, 559, 707, 847, 1046, 1053 |
| SfaNI | GCATC | 3 cut(s) | 115, 773, 795 |
| SfcI | CTRYAG | 1 cut(s) | 315 |
| SfoI | GGCGCC | 1 cut(s) | 753 |
| SinI | GGWCC | 2 cut(s) | 702, 811 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 134, 309 |
| Sse9I | AATT | 8 cut(s) | 235, 515, 630, 659, 898, 924, 1079, 1094 |
| SspDI | GGCGCC | 1 cut(s) | 751 |
| SspI | AATATT | 1 cut(s) | 647 |
| SspMI | CTAG | 2 cut(s) | 167, 299 |
| SstI | GAGCTC | 1 cut(s) | 1053 |
| StyD4I | CCNGG | 3 cut(s) | 86, 184, 606 |
| StyI | CCWWGG | 1 cut(s) | 543 |
| TaaI | ACNGT | 2 cut(s) | 143, 917 |
| TaqI | TCGA | 4 cut(s) | 220, 489, 528, 827 |
| TasI | AATT | 8 cut(s) | 235, 515, 630, 659, 898, 924, 1079, 1094 |
| TauI | GCSGC | 4 cut(s) | 201, 722, 866, 1031 |
| TfiI | GAWTC | 1 cut(s) | 939 |
| Tru1I | TTAA | 1 cut(s) | 923 |
| Tru9I | TTAA | 1 cut(s) | 923 |
| TscAI | CASTG | 3 cut(s) | 311, 481, 697 |
| TseI | GCWGC | 3 cut(s) | 314, 383, 561 |
| TspDTI | ATGAA | 1 cut(s) | 567 |
| TspRI | CASTG | 3 cut(s) | 311, 481, 697 |
| Tth111I | GACNNNGTC | 1 cut(s) | 524 |
| VneI | GTGCAC | 1 cut(s) | 255 |
| VpaK11BI | GGWCC | 2 cut(s) | 702, 811 |
| XapI | RAATTY | 1 cut(s) | 659 |
| XceI | RCATGY | 2 cut(s) | 768, 910 |
| XmiI | GTMKAC | 1 cut(s) | 528 |
| XspI | CTAG | 2 cut(s) | 167, 299 |
| Zsp2I | ATGCAT | 1 cut(s) | 788 |