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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 231, 822 |
| Acc16I | TGCGCA | 1 cut(s) | 981 |
| Acc36I | ACCTGC | 2 cut(s) | 373, 502 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 26, 59 |
| AccII | CGCG | 4 cut(s) | 36, 759, 888, 1230 |
| AciI | CCGC | 7 cut(s) | 134, 147, 160, 482, 528, 705, 1257 |
| AclI | AACGTT | 1 cut(s) | 648 |
| AclWI | GGATC | 3 cut(s) | 185, 320, 647 |
| AcoI | YGGCCR | 2 cut(s) | 61, 525 |
| AcuI | CTGAAG | 4 cut(s) | 120, 426, 648, 1106 |
| AcyI | GRCGYC | 1 cut(s) | 1266 |
| AfaI | GTAC | 4 cut(s) | 68, 558, 729, 762 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 26, 59, 382, 473, 632, 1008, 1009 |
| AflII | CTTAAG | 1 cut(s) | 689 |
| AgsI | TTSAA | 3 cut(s) | 107, 322, 488 |
| AjiI | CACGTC | 1 cut(s) | 595 |
| AjnI | CCWGG | 6 cut(s) | 6, 444, 472, 797, 841, 996 |
| AluBI | AGCT | 8 cut(s) | 272, 399, 542, 584, 755, 971, 1220, 1226 |
| AluI | AGCT | 8 cut(s) | 272, 399, 542, 584, 755, 971, 1220, 1226 |
| Alw26I | GTCTC | 1 cut(s) | 1068 |
| AlwI | GGATC | 3 cut(s) | 185, 320, 647 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 59, 275, 674 |
| ApeKI | GCWGC | 6 cut(s) | 272, 707, 838, 968, 971, 1259 |
| AseI | ATTAAT | 1 cut(s) | 957 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 911 |
| AspLEI | GCGC | 6 cut(s) | 38, 168, 415, 499, 566, 982 |
| AsuC2I | CCSGG | 2 cut(s) | 780, 1150 |
| AsuHPI | GGTGA | 5 cut(s) | 290, 599, 655, 781, 803 |
| AsuNHI | GCTAGC | 1 cut(s) | 751 |
| AvaII | GGWCC | 3 cut(s) | 240, 1104, 1237 |
| BaeGI | GKGCMC | 1 cut(s) | 312 |
| BalI | TGGCCA | 1 cut(s) | 63 |
| BanII | GRGCYC | 2 cut(s) | 687, 862 |
| BauI | CACGAG | 1 cut(s) | 1038 |
| BbsI | GAAGAC | 1 cut(s) | 597 |
| BbvCI | CCTCAGC | 1 cut(s) | 268 |
| BbvI | GCAGC | 6 cut(s) | 259, 694, 825, 958, 980, 1246 |
| BccI | CCATC | 2 cut(s) | 860, 1104 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 340, 374 |
| BciT130I | CCWGG | 6 cut(s) | 8, 446, 474, 799, 843, 998 |
| BcnI | CCSGG | 2 cut(s) | 780, 1150 |
| BcoDI | GTCTC | 1 cut(s) | 1068 |
| BfaI | CTAG | 3 cut(s) | 752, 1187, 1217 |
| BfmI | CTRYAG | 1 cut(s) | 935 |
| BfoI | RGCGCY | 1 cut(s) | 567 |
| BfrI | CTTAAG | 1 cut(s) | 689 |
| BfuAI | ACCTGC | 2 cut(s) | 373, 502 |
| BglII | AGATCT | 1 cut(s) | 1117 |
| BisI | GCNGC | 9 cut(s) | 147, 273, 528, 708, 839, 969, 972, 1257, 1260 |
| BlsI | GCNGC | 9 cut(s) | 148, 274, 529, 709, 840, 970, 973, 1258, 1261 |
| BmcAI | AGTACT | 1 cut(s) | 558 |
| Bme1390I | CCNGG | 8 cut(s) | 8, 446, 474, 780, 799, 843, 998, 1150 |
| Bme18I | GGWCC | 3 cut(s) | 240, 1104, 1237 |
| BmgBI | CACGTC | 1 cut(s) | 595 |
| BmrFI | CCNGG | 8 cut(s) | 8, 446, 474, 780, 799, 843, 998, 1150 |
| BmrI | ACTGGG | 3 cut(s) | 264, 371, 943 |
| BmsI | GCATC | 1 cut(s) | 400 |
| BmtI | GCTAGC | 1 cut(s) | 755 |
| BmuI | ACTGGG | 3 cut(s) | 264, 371, 943 |
| BpiI | GAAGAC | 1 cut(s) | 597 |
| BpmI | CTGGAG | 2 cut(s) | 683, 1196 |
| Bpu10I | CCTNAGC | 1 cut(s) | 268 |
| BpuEI | CTTGAG | 5 cut(s) | 229, 785, 892, 1175, 1206 |
| BpuMI | CCSGG | 2 cut(s) | 780, 1150 |
| BsaBI | GATNNNNATC | 1 cut(s) | 506 |
| BsaHI | GRCGYC | 1 cut(s) | 1266 |
| BsaJI | CCNNGG | 4 cut(s) | 7, 194, 798, 996 |
| BsaWI | WCCGGW | 1 cut(s) | 1101 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 277, 307, 627, 657, 927, 957 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 26, 59, 382, 473, 632, 1008, 1009 |
| Bse8I | GATNNNNATC | 1 cut(s) | 506 |
| BseBI | CCWGG | 6 cut(s) | 8, 446, 474, 799, 843, 998 |
| BseDI | CCNNGG | 4 cut(s) | 7, 194, 798, 996 |
| BseGI | GGATG | 1 cut(s) | 848 |
| BseJI | GATNNNNATC | 1 cut(s) | 506 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 26, 59, 382, 473, 632, 1008, 1009 |
| BseMII | CTCAG | 4 cut(s) | 282, 894, 1209, 1223 |
| BseRI | GAGGAG | 2 cut(s) | 765, 1024 |
| BseSI | GKGCMC | 1 cut(s) | 312 |
| BseX3I | CGGCCG | 1 cut(s) | 525 |
| BseXI | GCAGC | 6 cut(s) | 259, 694, 825, 958, 980, 1246 |
| BseYI | CCCAGC | 2 cut(s) | 52, 580 |
| BsgI | GTGCAG | 1 cut(s) | 401 |
| Bsh1236I | CGCG | 4 cut(s) | 36, 759, 888, 1230 |
| Bsh1285I | CGRYCG | 1 cut(s) | 528 |
| BsiEI | CGRYCG | 1 cut(s) | 528 |
| BsiSI | CCGG | 4 cut(s) | 524, 780, 1102, 1150 |
| BslFI | GGGAC | 3 cut(s) | 810, 815, 1090 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 26, 59, 382, 473, 632, 1008, 1009 |
| BsmAI | GTCTC | 1 cut(s) | 1068 |
| BsmFI | GGGAC | 3 cut(s) | 810, 815, 1090 |
| Bsp1286I | GDGCHC | 3 cut(s) | 312, 687, 862 |
| Bsp143I | GATC | 5 cut(s) | 190, 220, 325, 652, 1117 |
| Bsp68I | TCGCGA | 2 cut(s) | 888, 1230 |
| BspACI | CCGC | 7 cut(s) | 134, 147, 160, 482, 528, 705, 1257 |
| BspCNI | CTCAG | 4 cut(s) | 281, 895, 1208, 1222 |
| BspFNI | CGCG | 4 cut(s) | 36, 759, 888, 1230 |
| BspHI | TCATGA | 1 cut(s) | 418 |
| BspMI | ACCTGC | 2 cut(s) | 373, 502 |
| BspOI | GCTAGC | 1 cut(s) | 755 |
| BspPI | GGATC | 3 cut(s) | 185, 320, 647 |
| BspTI | CTTAAG | 1 cut(s) | 689 |
| BssECI | CCNNGG | 4 cut(s) | 7, 194, 798, 996 |
| BssMI | GATC | 5 cut(s) | 190, 220, 325, 652, 1117 |
| BssNI | GRCGYC | 1 cut(s) | 1266 |
| BssSI | CACGAG | 1 cut(s) | 1038 |
| Bst2BI | CACGAG | 1 cut(s) | 1038 |
| Bst2UI | CCWGG | 6 cut(s) | 8, 446, 474, 799, 843, 998 |
| Bst4CI | ACNGT | 1 cut(s) | 85 |
| Bst6I | CTCTTC | 1 cut(s) | 121 |
| BstACI | GRCGYC | 1 cut(s) | 1266 |
| BstAFI | CTTAAG | 1 cut(s) | 689 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 44 |
| BstDEI | CTNAG | 5 cut(s) | 154, 268, 903, 1195, 1209 |
| BstDSI | CCRYGG | 1 cut(s) | 194 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 630 |
| BstF5I | GGATG | 1 cut(s) | 848 |
| BstFNI | CGCG | 4 cut(s) | 36, 759, 888, 1230 |
| BstH2I | RGCGCY | 1 cut(s) | 567 |
| BstHHI | GCGC | 6 cut(s) | 38, 168, 415, 499, 566, 982 |
| BstKTI | GATC | 5 cut(s) | 193, 223, 328, 655, 1120 |
| BstMAI | GTCTC | 1 cut(s) | 1068 |
| BstMBI | GATC | 5 cut(s) | 190, 220, 325, 652, 1117 |
| BstMCI | CGRYCG | 1 cut(s) | 528 |
| BstNI | CCWGG | 6 cut(s) | 8, 446, 474, 799, 843, 998 |
| BstNSI | RCATGY | 1 cut(s) | 728 |
| BstSCI | CCNGG | 8 cut(s) | 6, 444, 472, 778, 797, 841, 996, 1148 |
| BstSFI | CTRYAG | 1 cut(s) | 935 |
| BstSLI | GKGCMC | 1 cut(s) | 312 |
| BstUI | CGCG | 4 cut(s) | 36, 759, 888, 1230 |
| BstV1I | GCAGC | 6 cut(s) | 259, 694, 825, 958, 980, 1246 |
| BstV2I | GAAGAC | 1 cut(s) | 597 |
| BstX2I | RGATCY | 1 cut(s) | 1117 |
| BstYI | RGATCY | 1 cut(s) | 1117 |
| BstZI | CGGCCG | 1 cut(s) | 525 |
| BtgI | CCRYGG | 1 cut(s) | 194 |
| BtrI | CACGTC | 1 cut(s) | 595 |
| BtsCI | GGATG | 1 cut(s) | 848 |
| BtsI | GCAGTG | 1 cut(s) | 719 |
| BtsIMutI | CAGTG | 6 cut(s) | 57, 312, 384, 663, 719, 956 |
| BtuMI | TCGCGA | 2 cut(s) | 888, 1230 |
| BveI | ACCTGC | 2 cut(s) | 373, 502 |
| CaiI | CAGNNNCTG | 3 cut(s) | 59, 275, 674 |
| CciI | TCATGA | 1 cut(s) | 418 |
| CfoI | GCGC | 6 cut(s) | 38, 168, 415, 499, 566, 982 |
| CseI | GACGC | 1 cut(s) | 1274 |
| Csp6I | GTAC | 4 cut(s) | 67, 557, 728, 761 |
| CviAII | CATG | 5 cut(s) | 419, 620, 725, 1160, 1307 |
| CviQI | GTAC | 4 cut(s) | 67, 557, 728, 761 |
| DdeI | CTNAG | 5 cut(s) | 154, 268, 903, 1195, 1209 |
| DpnI | GATC | 5 cut(s) | 192, 222, 327, 654, 1119 |
| DpnII | GATC | 5 cut(s) | 190, 220, 325, 652, 1117 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 231, 822 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 231, 822 |
| EaeI | YGGCCR | 2 cut(s) | 61, 525 |
| EagI | CGGCCG | 1 cut(s) | 525 |
| Eam1104I | CTCTTC | 1 cut(s) | 121 |
| EarI | CTCTTC | 1 cut(s) | 121 |
| EclXI | CGGCCG | 1 cut(s) | 525 |
| Eco24I | GRGCYC | 2 cut(s) | 687, 862 |
| Eco32I | GATATC | 1 cut(s) | 832 |
| Eco47I | GGWCC | 3 cut(s) | 240, 1104, 1237 |
| Eco52I | CGGCCG | 1 cut(s) | 525 |
| Eco57I | CTGAAG | 4 cut(s) | 120, 426, 648, 1106 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 630 |
| EcoO109I | RGGNCCY | 4 cut(s) | 240, 263, 458, 1012 |
| EcoRII | CCWGG | 6 cut(s) | 6, 444, 472, 797, 841, 996 |
| EcoRV | GATATC | 1 cut(s) | 832 |
| EcoT38I | GRGCYC | 2 cut(s) | 687, 862 |
| FaeI | CATG | 5 cut(s) | 422, 623, 728, 1163, 1310 |
| FaiI | YATR | 9 cut(s) | 237, 359, 389, 420, 437, 621, 726, 1161, 1308 |
| FaqI | GGGAC | 3 cut(s) | 810, 815, 1090 |
| FatI | CATG | 5 cut(s) | 418, 619, 724, 1159, 1306 |
| FauI | CCCGC | 2 cut(s) | 141, 167 |
| Fnu4HI | GCNGC | 9 cut(s) | 147, 273, 528, 708, 839, 969, 972, 1257, 1260 |
| FokI | GGATG | 1 cut(s) | 835 |
| FriOI | GRGCYC | 2 cut(s) | 687, 862 |
| Fsp4HI | GCNGC | 9 cut(s) | 147, 273, 528, 708, 839, 969, 972, 1257, 1260 |
| FspBI | CTAG | 3 cut(s) | 752, 1187, 1217 |
| FspI | TGCGCA | 1 cut(s) | 981 |
| GlaI | GCGC | 6 cut(s) | 37, 167, 414, 498, 565, 981 |
| GluI | GCNGC | 9 cut(s) | 147, 273, 528, 708, 839, 969, 972, 1257, 1260 |
| GsaI | CCCAGC | 2 cut(s) | 56, 584 |
| GsuI | CTGGAG | 2 cut(s) | 683, 1196 |
| HaeII | RGCGCY | 1 cut(s) | 567 |
| HapII | CCGG | 4 cut(s) | 524, 780, 1102, 1150 |
| HgaI | GACGC | 1 cut(s) | 1274 |
| HhaI | GCGC | 6 cut(s) | 38, 168, 415, 499, 566, 982 |
| Hin1I | GRCGYC | 1 cut(s) | 1266 |
| Hin1II | CATG | 5 cut(s) | 422, 623, 728, 1163, 1310 |
| Hin6I | GCGC | 6 cut(s) | 36, 166, 413, 497, 564, 980 |
| HinP1I | GCGC | 6 cut(s) | 36, 166, 413, 497, 564, 980 |
| HinfI | GANTC | 5 cut(s) | 110, 469, 772, 940, 1031 |
| HpaII | CCGG | 4 cut(s) | 524, 780, 1102, 1150 |
| HphI | GGTGA | 5 cut(s) | 290, 599, 655, 781, 803 |
| Hpy166II | GTNNAC | 1 cut(s) | 646 |
| Hpy188I | TCNGA | 6 cut(s) | 121, 178, 1083, 1198, 1212, 1241 |
| Hpy188III | TCNNGA | 9 cut(s) | 419, 488, 626, 738, 827, 863, 887, 1040, 1229 |
| Hpy8I | GTNNAC | 1 cut(s) | 646 |
| Hpy99I | CGWCG | 3 cut(s) | 125, 888, 1268 |
| HpyAV | CCTTC | 5 cut(s) | 254, 275, 521, 632, 744 |
| HpyCH4III | ACNGT | 1 cut(s) | 85 |
| HpyCH4IV | ACGT | 2 cut(s) | 594, 648 |
| HpyCH4V | TGCA | 5 cut(s) | 4, 361, 382, 724, 1310 |
| HpyF3I | CTNAG | 5 cut(s) | 154, 268, 903, 1195, 1209 |
| HpySE526I | ACGT | 2 cut(s) | 594, 648 |
| Hsp92I | GRCGYC | 1 cut(s) | 1266 |
| Hsp92II | CATG | 5 cut(s) | 422, 623, 728, 1163, 1310 |
| HspAI | GCGC | 6 cut(s) | 36, 166, 413, 497, 564, 980 |
| Kzo9I | GATC | 5 cut(s) | 190, 220, 325, 652, 1117 |
| LmnI | GCTCC | 6 cut(s) | 94, 298, 628, 682, 700, 1177 |
| Lsp1109I | GCAGC | 6 cut(s) | 259, 694, 825, 958, 980, 1246 |
| LweI | GCATC | 1 cut(s) | 400 |
| MaeI | CTAG | 3 cut(s) | 752, 1187, 1217 |
| MaeII | ACGT | 2 cut(s) | 594, 648 |
| MaeIII | GTNAC | 3 cut(s) | 769, 1040, 1141 |
| MalI | GATC | 5 cut(s) | 192, 222, 327, 654, 1119 |
| MboI | GATC | 5 cut(s) | 190, 220, 325, 652, 1117 |
| MboII | GAAGA | 7 cut(s) | 108, 119, 215, 419, 602, 872, 1112 |
| MfeI | CAATTG | 3 cut(s) | 141, 362, 1130 |
| MflI | RGATCY | 1 cut(s) | 1117 |
| MhlI | GDGCHC | 3 cut(s) | 312, 687, 862 |
| MlsI | TGGCCA | 1 cut(s) | 63 |
| MluCI | AATT | 3 cut(s) | 141, 362, 1130 |
| MluNI | TGGCCA | 1 cut(s) | 63 |
| MlyI | GAGTC | 2 cut(s) | 766, 1025 |
| MmeI | TCCRAC | 2 cut(s) | 144, 1264 |
| Mox20I | TGGCCA | 1 cut(s) | 63 |
| MroXI | GAANNNNTTC | 1 cut(s) | 911 |
| MscI | TGGCCA | 1 cut(s) | 63 |
| MseI | TTAA | 5 cut(s) | 42, 690, 821, 957, 1301 |
| Msp20I | TGGCCA | 1 cut(s) | 63 |
| MspA1I | CMGCKG | 5 cut(s) | 272, 584, 707, 971, 1259 |
| MspCI | CTTAAG | 1 cut(s) | 689 |
| MspI | CCGG | 4 cut(s) | 524, 780, 1102, 1150 |
| MspR9I | CCNGG | 8 cut(s) | 8, 446, 474, 780, 799, 843, 998, 1150 |
| MunI | CAATTG | 3 cut(s) | 141, 362, 1130 |
| MvaI | CCWGG | 6 cut(s) | 8, 446, 474, 799, 843, 998 |
| MvnI | CGCG | 4 cut(s) | 36, 759, 888, 1230 |
| NciI | CCSGG | 2 cut(s) | 780, 1150 |
| NdeII | GATC | 5 cut(s) | 190, 220, 325, 652, 1117 |
| NheI | GCTAGC | 1 cut(s) | 751 |
| NlaIII | CATG | 5 cut(s) | 422, 623, 728, 1163, 1310 |
| NmuCI | GTSAC | 3 cut(s) | 769, 1040, 1141 |
| NruI | TCGCGA | 2 cut(s) | 888, 1230 |
| NsbI | TGCGCA | 1 cut(s) | 981 |
| NspI | RCATGY | 1 cut(s) | 728 |
| PagI | TCATGA | 1 cut(s) | 418 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 229, 889 |
| PdmI | GAANNNNTTC | 1 cut(s) | 911 |
| PfeI | GAWTC | 3 cut(s) | 110, 469, 940 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 26, 59 |
| PfoI | TCCNGGA | 1 cut(s) | 778 |
| PkrI | GCNGC | 9 cut(s) | 148, 274, 529, 709, 840, 970, 973, 1258, 1261 |
| PleI | GAGTC | 2 cut(s) | 766, 1025 |
| PpsI | GAGTC | 2 cut(s) | 766, 1025 |
| PpuMI | RGGWCCY | 1 cut(s) | 240 |
| PshBI | ATTAAT | 1 cut(s) | 957 |
| Psp1406I | AACGTT | 1 cut(s) | 648 |
| Psp5II | RGGWCCY | 1 cut(s) | 240 |
| Psp6I | CCWGG | 6 cut(s) | 6, 444, 472, 797, 841, 996 |
| PspFI | CCCAGC | 2 cut(s) | 52, 580 |
| PspGI | CCWGG | 6 cut(s) | 6, 444, 472, 797, 841, 996 |
| PspPPI | RGGWCCY | 1 cut(s) | 240 |
| PstNI | CAGNNNCTG | 3 cut(s) | 59, 275, 674 |
| PsuI | RGATCY | 1 cut(s) | 1117 |
| PvuII | CAGCTG | 3 cut(s) | 272, 584, 971 |
| RruI | TCGCGA | 2 cut(s) | 888, 1230 |
| RsaI | GTAC | 4 cut(s) | 68, 558, 729, 762 |
| RsaNI | GTAC | 4 cut(s) | 67, 557, 728, 761 |
| SaqAI | TTAA | 5 cut(s) | 42, 690, 821, 957, 1301 |
| SatI | GCNGC | 9 cut(s) | 147, 273, 528, 708, 839, 969, 972, 1257, 1260 |
| Sau3AI | GATC | 5 cut(s) | 190, 220, 325, 652, 1117 |
| ScaI | AGTACT | 1 cut(s) | 558 |
| SchI | GAGTC | 2 cut(s) | 766, 1025 |
| ScrFI | CCNGG | 8 cut(s) | 8, 446, 474, 780, 799, 843, 998, 1150 |
| SduI | GDGCHC | 3 cut(s) | 312, 687, 862 |
| SfaNI | GCATC | 1 cut(s) | 400 |
| SfcI | CTRYAG | 1 cut(s) | 935 |
| SinI | GGWCC | 3 cut(s) | 240, 1104, 1237 |
| SmlI | CTYRAG | 6 cut(s) | 244, 689, 764, 871, 1154, 1221 |
| SmoI | CTYRAG | 6 cut(s) | 244, 689, 764, 871, 1154, 1221 |
| Sse9I | AATT | 3 cut(s) | 141, 362, 1130 |
| SsiI | CCGC | 7 cut(s) | 134, 147, 160, 482, 528, 705, 1257 |
| SspI | AATATT | 1 cut(s) | 1203 |
| SspMI | CTAG | 3 cut(s) | 752, 1187, 1217 |
| StyD4I | CCNGG | 8 cut(s) | 6, 444, 472, 778, 797, 841, 996, 1148 |
| TaaI | ACNGT | 1 cut(s) | 85 |
| TaiI | ACGT | 2 cut(s) | 597, 651 |
| TaqI | TCGA | 2 cut(s) | 184, 223 |
| TasI | AATT | 3 cut(s) | 141, 362, 1130 |
| TatI | WGTACW | 2 cut(s) | 66, 556 |
| TauI | GCSGC | 3 cut(s) | 149, 530, 1259 |
| TfiI | GAWTC | 3 cut(s) | 110, 469, 940 |
| Tru1I | TTAA | 5 cut(s) | 42, 690, 821, 957, 1301 |
| Tru9I | TTAA | 5 cut(s) | 42, 690, 821, 957, 1301 |
| TscAI | CASTG | 6 cut(s) | 64, 319, 384, 663, 726, 956 |
| TseFI | GTSAC | 3 cut(s) | 769, 1040, 1141 |
| TseI | GCWGC | 6 cut(s) | 272, 707, 838, 968, 971, 1259 |
| Tsp45I | GTSAC | 3 cut(s) | 769, 1040, 1141 |
| TspDTI | ATGAA | 4 cut(s) | 222, 807, 904, 975 |
| TspGWI | ACGGA | 3 cut(s) | 107, 183, 819 |
| TspRI | CASTG | 6 cut(s) | 64, 319, 384, 663, 726, 956 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 26, 59 |
| Vha464I | CTTAAG | 1 cut(s) | 689 |
| VpaK11BI | GGWCC | 3 cut(s) | 240, 1104, 1237 |
| VspI | ATTAAT | 1 cut(s) | 957 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 630 |
| XceI | RCATGY | 1 cut(s) | 728 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 311, 442, 859, 1276 |
| XmnI | GAANNNNTTC | 1 cut(s) | 911 |
| XspI | CTAG | 3 cut(s) | 752, 1187, 1217 |
| ZrmI | AGTACT | 1 cut(s) | 558 |