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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 594 |
| AccB1I | GGYRCC | 1 cut(s) | 458 |
| AccII | CGCG | 6 cut(s) | 89, 206, 490, 540, 542, 670 |
| AclWI | GGATC | 1 cut(s) | 635 |
| AcoI | YGGCCR | 2 cut(s) | 225, 356 |
| AcsI | RAATTY | 1 cut(s) | 648 |
| AcuI | CTGAAG | 1 cut(s) | 458 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 11, 188, 200, 218, 519, 601 |
| AgeI | ACCGGT | 1 cut(s) | 455 |
| AgsI | TTSAA | 1 cut(s) | 698 |
| AjnI | CCWGG | 1 cut(s) | 353 |
| AluBI | AGCT | 3 cut(s) | 82, 136, 536 |
| AluI | AGCT | 3 cut(s) | 82, 136, 536 |
| AlwI | GGATC | 1 cut(s) | 635 |
| Ama87I | CYCGRG | 3 cut(s) | 252, 545, 559 |
| AoxI | GGCC | 8 cut(s) | 190, 220, 225, 356, 365, 521, 576, 640 |
| ApoI | RAATTY | 1 cut(s) | 648 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 68, 100 |
| AsiGI | ACCGGT | 1 cut(s) | 455 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 693 |
| AspLEI | GCGC | 4 cut(s) | 206, 492, 542, 544 |
| AspS9I | GGNCC | 7 cut(s) | 14, 191, 221, 366, 530, 605, 641 |
| AsuC2I | CCSGG | 6 cut(s) | 224, 369, 560, 561, 580, 645 |
| AsuHPI | GGTGA | 4 cut(s) | 383, 439, 562, 584 |
| AvaI | CYCGRG | 3 cut(s) | 252, 545, 559 |
| AvaII | GGWCC | 3 cut(s) | 14, 530, 605 |
| BanI | GGYRCC | 1 cut(s) | 458 |
| BanII | GRGCYC | 1 cut(s) | 212 |
| BccI | CCATC | 2 cut(s) | 4, 578 |
| BceAI | ACGGC | 1 cut(s) | 446 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 122, 156 |
| BciT130I | CCWGG | 1 cut(s) | 355 |
| BcnI | CCSGG | 6 cut(s) | 224, 369, 560, 561, 580, 645 |
| BfaI | CTAG | 1 cut(s) | 519 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 364 |
| BisI | GCNGC | 5 cut(s) | 340, 343, 346, 485, 488 |
| BlsI | GCNGC | 5 cut(s) | 341, 344, 347, 486, 489 |
| Bme1390I | CCNGG | 7 cut(s) | 224, 355, 369, 560, 561, 580, 645 |
| Bme18I | GGWCC | 3 cut(s) | 14, 530, 605 |
| BmeT110I | CYCGRG | 3 cut(s) | 252, 545, 559 |
| BmgT120I | GGNCC | 7 cut(s) | 14, 191, 221, 366, 530, 605, 641 |
| BmiI | GGNNCC | 9 cut(s) | 15, 65, 105, 193, 460, 468, 606, 607, 643 |
| BmrFI | CCNGG | 7 cut(s) | 224, 355, 369, 560, 561, 580, 645 |
| BmsI | GCATC | 2 cut(s) | 102, 148 |
| BpuMI | CCSGG | 6 cut(s) | 224, 369, 560, 561, 580, 645 |
| BsaJI | CCNNGG | 6 cut(s) | 5, 462, 558, 559, 579, 643 |
| BsaWI | WCCGGW | 1 cut(s) | 455 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 11, 188, 200, 218, 519, 601 |
| Bse118I | RCCGGY | 5 cut(s) | 66, 188, 200, 227, 455 |
| BseBI | CCWGG | 1 cut(s) | 355 |
| BseDI | CCNNGG | 6 cut(s) | 5, 462, 558, 559, 579, 643 |
| BseGI | GGATG | 2 cut(s) | 15, 589 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 11, 188, 200, 218, 519, 601 |
| BsePI | GCGCGC | 1 cut(s) | 540 |
| BseX3I | CGGCCG | 1 cut(s) | 225 |
| Bsh1236I | CGCG | 6 cut(s) | 89, 206, 490, 540, 542, 670 |
| Bsh1285I | CGRYCG | 2 cut(s) | 228, 456 |
| BshFI | GGCC | 8 cut(s) | 192, 222, 227, 358, 367, 523, 578, 642 |
| BshNI | GGYRCC | 1 cut(s) | 458 |
| BshTI | ACCGGT | 1 cut(s) | 455 |
| BsiEI | CGRYCG | 2 cut(s) | 228, 456 |
| BsiHKCI | CYCGRG | 3 cut(s) | 252, 545, 559 |
| BslFI | GGGAC | 2 cut(s) | 27, 591 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 11, 188, 200, 218, 519, 601 |
| BsmFI | GGGAC | 2 cut(s) | 27, 591 |
| BsnI | GGCC | 8 cut(s) | 192, 222, 227, 358, 367, 523, 578, 642 |
| BsoBI | CYCGRG | 3 cut(s) | 252, 545, 559 |
| Bsp1286I | GDGCHC | 1 cut(s) | 212 |
| Bsp143I | GATC | 1 cut(s) | 627 |
| Bsp68I | TCGCGA | 2 cut(s) | 89, 670 |
| BspANI | GGCC | 8 cut(s) | 192, 222, 227, 358, 367, 523, 578, 642 |
| BspFNI | CGCG | 6 cut(s) | 89, 206, 490, 540, 542, 670 |
| BspLI | GGNNCC | 9 cut(s) | 15, 65, 105, 193, 460, 468, 606, 607, 643 |
| BspPI | GGATC | 1 cut(s) | 635 |
| BspT107I | GGYRCC | 1 cut(s) | 458 |
| BsrFI | RCCGGY | 5 cut(s) | 66, 188, 200, 227, 455 |
| BssAI | RCCGGY | 5 cut(s) | 66, 188, 200, 227, 455 |
| BssECI | CCNNGG | 6 cut(s) | 5, 462, 558, 559, 579, 643 |
| BssHII | GCGCGC | 1 cut(s) | 540 |
| BssMI | GATC | 1 cut(s) | 627 |
| Bst2UI | CCWGG | 1 cut(s) | 355 |
| Bst4CI | ACNGT | 1 cut(s) | 596 |
| Bst6I | CTCTTC | 1 cut(s) | 477 |
| BstC8I | GCNNGC | 9 cut(s) | 68, 91, 145, 208, 538, 542, 621, 672, 676 |
| BstDSI | CCRYGG | 2 cut(s) | 5, 462 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 517 |
| BstF5I | GGATG | 2 cut(s) | 15, 589 |
| BstFNI | CGCG | 6 cut(s) | 89, 206, 490, 540, 542, 670 |
| BstHHI | GCGC | 4 cut(s) | 206, 492, 542, 544 |
| BstKTI | GATC | 1 cut(s) | 630 |
| BstMBI | GATC | 1 cut(s) | 627 |
| BstMCI | CGRYCG | 2 cut(s) | 228, 456 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 203, 345, 364, 458, 475, 484, 629 |
| BstNI | CCWGG | 1 cut(s) | 355 |
| BstNSI | RCATGY | 1 cut(s) | 678 |
| BstSCI | CCNGG | 7 cut(s) | 222, 353, 367, 558, 559, 578, 643 |
| BstUI | CGCG | 6 cut(s) | 89, 206, 490, 540, 542, 670 |
| BstZI | CGGCCG | 1 cut(s) | 225 |
| BsuRI | GGCC | 8 cut(s) | 192, 222, 227, 358, 367, 523, 578, 642 |
| BtgI | CCRYGG | 2 cut(s) | 5, 462 |
| BtsCI | GGATG | 2 cut(s) | 15, 589 |
| BtuMI | TCGCGA | 2 cut(s) | 89, 670 |
| Cac8I | GCNNGC | 9 cut(s) | 68, 91, 145, 208, 538, 542, 621, 672, 676 |
| CfoI | GCGC | 4 cut(s) | 206, 492, 542, 544 |
| Cfr10I | RCCGGY | 5 cut(s) | 66, 188, 200, 227, 455 |
| Cfr13I | GGNCC | 7 cut(s) | 14, 191, 221, 366, 530, 605, 641 |
| Cfr9I | CCCGGG | 1 cut(s) | 559 |
| CspAI | ACCGGT | 1 cut(s) | 455 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 249, 284 |
| CviAII | CATG | 1 cut(s) | 675 |
| DpnI | GATC | 1 cut(s) | 629 |
| DpnII | GATC | 1 cut(s) | 627 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 594 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 594 |
| EaeI | YGGCCR | 2 cut(s) | 225, 356 |
| EagI | CGGCCG | 1 cut(s) | 225 |
| Eam1104I | CTCTTC | 1 cut(s) | 477 |
| EarI | CTCTTC | 1 cut(s) | 477 |
| EclXI | CGGCCG | 1 cut(s) | 225 |
| Eco24I | GRGCYC | 1 cut(s) | 212 |
| Eco32I | GATATC | 1 cut(s) | 613 |
| Eco47I | GGWCC | 3 cut(s) | 14, 530, 605 |
| Eco52I | CGGCCG | 1 cut(s) | 225 |
| Eco57I | CTGAAG | 1 cut(s) | 458 |
| Eco88I | CYCGRG | 3 cut(s) | 252, 545, 559 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 517 |
| EcoO109I | RGGNCCY | 1 cut(s) | 605 |
| EcoRII | CCWGG | 1 cut(s) | 353 |
| EcoRV | GATATC | 1 cut(s) | 613 |
| EcoT38I | GRGCYC | 1 cut(s) | 212 |
| FaeI | CATG | 1 cut(s) | 678 |
| FaiI | YATR | 5 cut(s) | 29, 75, 120, 233, 676 |
| FaqI | GGGAC | 2 cut(s) | 27, 591 |
| FatI | CATG | 1 cut(s) | 674 |
| FauI | CCCGC | 4 cut(s) | 180, 202, 489, 649 |
| Fnu4HI | GCNGC | 5 cut(s) | 340, 343, 346, 485, 488 |
| FokI | GGATG | 2 cut(s) | 22, 596 |
| FriOI | GRGCYC | 1 cut(s) | 212 |
| Fsp4HI | GCNGC | 5 cut(s) | 340, 343, 346, 485, 488 |
| FspBI | CTAG | 1 cut(s) | 519 |
| GlaI | GCGC | 4 cut(s) | 205, 491, 541, 543 |
| GluI | GCNGC | 5 cut(s) | 340, 343, 346, 485, 488 |
| HaeIII | GGCC | 8 cut(s) | 192, 222, 227, 358, 367, 523, 578, 642 |
| HhaI | GCGC | 4 cut(s) | 206, 492, 542, 544 |
| Hin1II | CATG | 1 cut(s) | 678 |
| Hin6I | GCGC | 4 cut(s) | 204, 490, 540, 542 |
| HinP1I | GCGC | 4 cut(s) | 204, 490, 540, 542 |
| HincII | GTYRAC | 1 cut(s) | 55 |
| HindII | GTYRAC | 1 cut(s) | 55 |
| HinfI | GANTC | 6 cut(s) | 238, 264, 314, 361, 392, 553 |
| HphI | GGTGA | 4 cut(s) | 383, 439, 562, 584 |
| Hpy166II | GTNNAC | 3 cut(s) | 55, 245, 430 |
| Hpy188I | TCNGA | 1 cut(s) | 627 |
| Hpy188III | TCNNGA | 7 cut(s) | 88, 152, 242, 254, 389, 608, 669 |
| Hpy8I | GTNNAC | 3 cut(s) | 55, 245, 430 |
| Hpy99I | CGWCG | 4 cut(s) | 294, 437, 639, 670 |
| HpyAV | CCTTC | 2 cut(s) | 16, 416 |
| HpyCH4III | ACNGT | 1 cut(s) | 596 |
| HpyCH4IV | ACGT | 1 cut(s) | 432 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 203, 345, 364, 458, 475, 484, 629 |
| HpySE526I | ACGT | 1 cut(s) | 432 |
| Hsp92II | CATG | 1 cut(s) | 678 |
| HspAI | GCGC | 4 cut(s) | 204, 490, 540, 542 |
| KflI | GGGWCCC | 1 cut(s) | 605 |
| KroI | GCCGGC | 1 cut(s) | 66 |
| KroNI | GCCGGC | 1 cut(s) | 68 |
| Kzo9I | GATC | 1 cut(s) | 627 |
| LmnI | GCTCC | 2 cut(s) | 63, 466 |
| LweI | GCATC | 2 cut(s) | 102, 148 |
| MaeI | CTAG | 1 cut(s) | 519 |
| MaeII | ACGT | 1 cut(s) | 432 |
| MaeIII | GTNAC | 1 cut(s) | 550 |
| MalI | GATC | 1 cut(s) | 629 |
| MboI | GATC | 1 cut(s) | 627 |
| MboII | GAAGA | 8 cut(s) | 31, 43, 167, 290, 345, 386, 464, 654 |
| MhlI | GDGCHC | 1 cut(s) | 212 |
| MluCI | AATT | 1 cut(s) | 648 |
| MlyI | GAGTC | 3 cut(s) | 355, 401, 547 |
| MnlI | CCTC | 6 cut(s) | 189, 261, 480, 523, 541, 568 |
| MroNI | GCCGGC | 1 cut(s) | 66 |
| MroXI | GAANNNNTTC | 1 cut(s) | 693 |
| MseI | TTAA | 1 cut(s) | 303 |
| MspR9I | CCNGG | 7 cut(s) | 224, 355, 369, 560, 561, 580, 645 |
| MvaI | CCWGG | 1 cut(s) | 355 |
| MvnI | CGCG | 6 cut(s) | 89, 206, 490, 540, 542, 670 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 203, 345, 364, 458, 475, 484, 629 |
| NaeI | GCCGGC | 1 cut(s) | 68 |
| NciI | CCSGG | 6 cut(s) | 224, 369, 560, 561, 580, 645 |
| NdeII | GATC | 1 cut(s) | 627 |
| NgoMIV | GCCGGC | 1 cut(s) | 66 |
| NlaIII | CATG | 1 cut(s) | 678 |
| NlaIV | GGNNCC | 9 cut(s) | 15, 65, 105, 193, 460, 468, 606, 607, 643 |
| NmeAIII | GCCGAG | 1 cut(s) | 343 |
| NmuCI | GTSAC | 1 cut(s) | 550 |
| NruI | TCGCGA | 2 cut(s) | 89, 670 |
| NspI | RCATGY | 1 cut(s) | 678 |
| PaeI | GCATGC | 1 cut(s) | 678 |
| PaeR7I | CTCGAG | 2 cut(s) | 252, 545 |
| PauI | GCGCGC | 1 cut(s) | 540 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 259, 449, 631 |
| PdiI | GCCGGC | 1 cut(s) | 68 |
| PdmI | GAANNNNTTC | 1 cut(s) | 693 |
| PfeI | GAWTC | 3 cut(s) | 238, 264, 314 |
| PinAI | ACCGGT | 1 cut(s) | 455 |
| PkrI | GCNGC | 5 cut(s) | 341, 344, 347, 486, 489 |
| PleI | GAGTC | 3 cut(s) | 355, 400, 547 |
| PpsI | GAGTC | 3 cut(s) | 355, 400, 547 |
| PpuMI | RGGWCCY | 1 cut(s) | 605 |
| Psp5II | RGGWCCY | 1 cut(s) | 605 |
| Psp6I | CCWGG | 1 cut(s) | 353 |
| PspGI | CCWGG | 1 cut(s) | 353 |
| PspN4I | GGNNCC | 9 cut(s) | 15, 65, 105, 193, 460, 468, 606, 607, 643 |
| PspPI | GGNCC | 7 cut(s) | 14, 191, 221, 366, 530, 605, 641 |
| PspPPI | RGGWCCY | 1 cut(s) | 605 |
| PspXI | VCTCGAGB | 1 cut(s) | 545 |
| PteI | GCGCGC | 1 cut(s) | 540 |
| RruI | TCGCGA | 2 cut(s) | 89, 670 |
| SaqAI | TTAA | 1 cut(s) | 303 |
| SatI | GCNGC | 5 cut(s) | 340, 343, 346, 485, 488 |
| Sau3AI | GATC | 1 cut(s) | 627 |
| Sau96I | GGNCC | 7 cut(s) | 14, 191, 221, 366, 530, 605, 641 |
| SchI | GAGTC | 3 cut(s) | 355, 401, 547 |
| ScrFI | CCNGG | 7 cut(s) | 224, 355, 369, 560, 561, 580, 645 |
| SduI | GDGCHC | 1 cut(s) | 212 |
| SetI | ASST | 5 cut(s) | 84, 138, 435, 538, 552 |
| SfaNI | GCATC | 2 cut(s) | 102, 148 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 364 |
| Sfr274I | CTCGAG | 2 cut(s) | 252, 545 |
| SinI | GGWCC | 3 cut(s) | 14, 530, 605 |
| SlaI | CTCGAG | 2 cut(s) | 252, 545 |
| SmaI | CCCGGG | 1 cut(s) | 561 |
| SmlI | CTYRAG | 2 cut(s) | 252, 545 |
| SmoI | CTYRAG | 2 cut(s) | 252, 545 |
| SphI | GCATGC | 1 cut(s) | 678 |
| Sse9I | AATT | 1 cut(s) | 648 |
| SspMI | CTAG | 1 cut(s) | 519 |
| StyD4I | CCNGG | 7 cut(s) | 222, 353, 367, 558, 559, 578, 643 |
| TaaI | ACNGT | 1 cut(s) | 596 |
| TaiI | ACGT | 1 cut(s) | 435 |
| TaqI | TCGA | 5 cut(s) | 132, 253, 289, 435, 546 |
| TaqII | GACCGA | 2 cut(s) | 31, 374 |
| TasI | AATT | 1 cut(s) | 648 |
| TauI | GCSGC | 5 cut(s) | 342, 345, 348, 487, 490 |
| TfiI | GAWTC | 3 cut(s) | 238, 264, 314 |
| Tru1I | TTAA | 1 cut(s) | 303 |
| Tru9I | TTAA | 1 cut(s) | 303 |
| TseFI | GTSAC | 1 cut(s) | 550 |
| Tsp45I | GTSAC | 1 cut(s) | 550 |
| TspDTI | ATGAA | 1 cut(s) | 44 |
| TspGWI | ACGGA | 1 cut(s) | 543 |
| TspMI | CCCGGG | 1 cut(s) | 559 |
| VpaK11BI | GGWCC | 3 cut(s) | 14, 530, 605 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 517 |
| XapI | RAATTY | 1 cut(s) | 648 |
| XceI | RCATGY | 1 cut(s) | 678 |
| XhoI | CTCGAG | 2 cut(s) | 252, 545 |
| XmaI | CCCGGG | 1 cut(s) | 559 |
| XmnI | GAANNNNTTC | 1 cut(s) | 693 |
| XspI | CTAG | 1 cut(s) | 519 |