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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 319 |
| Acc36I | ACCTGC | 1 cut(s) | 479 |
| AccBSI | CCGCTC | 1 cut(s) | 221 |
| AccII | CGCG | 9 cut(s) | 150, 256, 345, 347, 385, 471, 477, 538, 748 |
| AclWI | GGATC | 1 cut(s) | 45 |
| AfaI | GTAC | 1 cut(s) | 259 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 185, 569 |
| AgsI | TTSAA | 1 cut(s) | 111 |
| AjnI | CCWGG | 2 cut(s) | 234, 275 |
| AluBI | AGCT | 8 cut(s) | 39, 120, 357, 428, 483, 558, 617, 774 |
| AluI | AGCT | 8 cut(s) | 39, 120, 357, 428, 483, 558, 617, 774 |
| Alw21I | GWGCWC | 1 cut(s) | 359 |
| Alw26I | GTCTC | 2 cut(s) | 522, 730 |
| AlwI | GGATC | 1 cut(s) | 45 |
| Ama87I | CYCGRG | 1 cut(s) | 222 |
| AoxI | GGCC | 7 cut(s) | 151, 403, 509, 622, 643, 664, 830 |
| ApeKI | GCWGC | 3 cut(s) | 183, 189, 774 |
| AseI | ATTAAT | 1 cut(s) | 454 |
| AspLEI | GCGC | 3 cut(s) | 345, 347, 479 |
| AspS9I | GGNCC | 6 cut(s) | 152, 321, 601, 623, 644, 665 |
| AsuHPI | GGTGA | 3 cut(s) | 389, 404, 671 |
| AsuII | TTCGAA | 1 cut(s) | 715 |
| AvaI | CYCGRG | 1 cut(s) | 222 |
| AvaII | GGWCC | 2 cut(s) | 321, 601 |
| BanII | GRGCYC | 1 cut(s) | 359 |
| BauI | CACGAG | 1 cut(s) | 612 |
| Bbv12I | GWGCWC | 1 cut(s) | 359 |
| BbvI | GCAGC | 3 cut(s) | 170, 176, 761 |
| BccI | CCATC | 4 cut(s) | 165, 294, 365, 402 |
| BceAI | ACGGC | 1 cut(s) | 679 |
| BciT130I | CCWGG | 2 cut(s) | 236, 277 |
| BcoDI | GTCTC | 2 cut(s) | 522, 730 |
| BfaI | CTAG | 5 cut(s) | 63, 339, 484, 693, 787 |
| BfmI | CTRYAG | 1 cut(s) | 306 |
| BfuAI | ACCTGC | 1 cut(s) | 479 |
| BisI | GCNGC | 7 cut(s) | 148, 151, 184, 187, 190, 383, 775 |
| BlsI | GCNGC | 7 cut(s) | 149, 152, 185, 188, 191, 384, 776 |
| Bme1390I | CCNGG | 2 cut(s) | 236, 277 |
| Bme18I | GGWCC | 2 cut(s) | 321, 601 |
| BmeT110I | CYCGRG | 1 cut(s) | 222 |
| BmgT120I | GGNCC | 6 cut(s) | 152, 321, 601, 623, 644, 665 |
| BmiI | GGNNCC | 4 cut(s) | 142, 154, 322, 685 |
| BmrFI | CCNGG | 2 cut(s) | 236, 277 |
| BmrI | ACTGGG | 1 cut(s) | 641 |
| BmsI | GCATC | 1 cut(s) | 194 |
| BmuI | ACTGGG | 1 cut(s) | 641 |
| BpmI | CTGGAG | 1 cut(s) | 261 |
| Bpu10I | CCTNAGC | 1 cut(s) | 358 |
| Bpu14I | TTCGAA | 1 cut(s) | 715 |
| BpuEI | CTTGAG | 4 cut(s) | 70, 154, 693, 724 |
| BsaJI | CCNNGG | 3 cut(s) | 148, 400, 512 |
| BsaWI | WCCGGW | 1 cut(s) | 137 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 185, 569 |
| Bse1I | ACTGG | 1 cut(s) | 647 |
| Bse3DI | GCAATG | 1 cut(s) | 727 |
| BseBI | CCWGG | 2 cut(s) | 236, 277 |
| BseDI | CCNNGG | 3 cut(s) | 148, 400, 512 |
| BseGI | GGATG | 3 cut(s) | 272, 305, 806 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 185, 569 |
| BseMI | GCAATG | 1 cut(s) | 727 |
| BseMII | CTCAG | 3 cut(s) | 4, 372, 741 |
| BseNI | ACTGG | 1 cut(s) | 647 |
| BsePI | GCGCGC | 1 cut(s) | 343 |
| BseXI | GCAGC | 3 cut(s) | 170, 176, 761 |
| BseYI | CCCAGC | 1 cut(s) | 120 |
| Bsh1236I | CGCG | 9 cut(s) | 150, 256, 345, 347, 385, 471, 477, 538, 748 |
| Bsh1285I | CGRYCG | 2 cut(s) | 449, 475 |
| BshFI | GGCC | 7 cut(s) | 153, 405, 511, 624, 645, 666, 832 |
| BsiEI | CGRYCG | 2 cut(s) | 449, 475 |
| BsiHKAI | GWGCWC | 1 cut(s) | 359 |
| BsiHKCI | CYCGRG | 1 cut(s) | 222 |
| BsiSI | CCGG | 1 cut(s) | 138 |
| BslFI | GGGAC | 1 cut(s) | 585 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 185, 569 |
| BsmAI | GTCTC | 2 cut(s) | 522, 730 |
| BsmFI | GGGAC | 1 cut(s) | 585 |
| BsnI | GGCC | 7 cut(s) | 153, 405, 511, 624, 645, 666, 832 |
| BsoBI | CYCGRG | 1 cut(s) | 222 |
| Bsp119I | TTCGAA | 1 cut(s) | 715 |
| Bsp1286I | GDGCHC | 1 cut(s) | 359 |
| Bsp143I | GATC | 3 cut(s) | 50, 59, 446 |
| Bsp68I | TCGCGA | 1 cut(s) | 538 |
| BspANI | GGCC | 7 cut(s) | 153, 405, 511, 624, 645, 666, 832 |
| BspCNI | CTCAG | 3 cut(s) | 5, 371, 740 |
| BspFNI | CGCG | 9 cut(s) | 150, 256, 345, 347, 385, 471, 477, 538, 748 |
| BspLI | GGNNCC | 4 cut(s) | 142, 154, 322, 685 |
| BspMI | ACCTGC | 1 cut(s) | 479 |
| BspPI | GGATC | 1 cut(s) | 45 |
| BspT104I | TTCGAA | 1 cut(s) | 715 |
| BsrBI | CCGCTC | 1 cut(s) | 221 |
| BsrDI | GCAATG | 1 cut(s) | 727 |
| BsrI | ACTGG | 1 cut(s) | 647 |
| BssECI | CCNNGG | 3 cut(s) | 148, 400, 512 |
| BssHII | GCGCGC | 1 cut(s) | 343 |
| BssMI | GATC | 3 cut(s) | 50, 59, 446 |
| BssSI | CACGAG | 1 cut(s) | 612 |
| BssT1I | CCWWGG | 1 cut(s) | 512 |
| Bst2BI | CACGAG | 1 cut(s) | 612 |
| Bst2UI | CCWGG | 2 cut(s) | 236, 277 |
| Bst4CI | ACNGT | 1 cut(s) | 32 |
| BstBI | TTCGAA | 1 cut(s) | 715 |
| BstC8I | GCNNGC | 8 cut(s) | 94, 145, 345, 349, 481, 746, 772, 830 |
| BstDEI | CTNAG | 3 cut(s) | 13, 358, 727 |
| BstDSI | CCRYGG | 1 cut(s) | 148 |
| BstF5I | GGATG | 3 cut(s) | 272, 305, 806 |
| BstFNI | CGCG | 9 cut(s) | 150, 256, 345, 347, 385, 471, 477, 538, 748 |
| BstHHI | GCGC | 3 cut(s) | 345, 347, 479 |
| BstKTI | GATC | 3 cut(s) | 53, 62, 449 |
| BstMAI | GTCTC | 2 cut(s) | 522, 730 |
| BstMBI | GATC | 3 cut(s) | 50, 59, 446 |
| BstMCI | CGRYCG | 2 cut(s) | 449, 475 |
| BstNI | CCWGG | 2 cut(s) | 236, 277 |
| BstSCI | CCNGG | 2 cut(s) | 234, 275 |
| BstSFI | CTRYAG | 1 cut(s) | 306 |
| BstUI | CGCG | 9 cut(s) | 150, 256, 345, 347, 385, 471, 477, 538, 748 |
| BstV1I | GCAGC | 3 cut(s) | 170, 176, 761 |
| BsuRI | GGCC | 7 cut(s) | 153, 405, 511, 624, 645, 666, 832 |
| BtgI | CCRYGG | 1 cut(s) | 148 |
| BtsCI | GGATG | 3 cut(s) | 272, 305, 806 |
| BtsIMutI | CAGTG | 1 cut(s) | 199 |
| BtuMI | TCGCGA | 1 cut(s) | 538 |
| BveI | ACCTGC | 1 cut(s) | 479 |
| Cac8I | GCNNGC | 8 cut(s) | 94, 145, 345, 349, 481, 746, 772, 830 |
| CfoI | GCGC | 3 cut(s) | 345, 347, 479 |
| Cfr13I | GGNCC | 6 cut(s) | 152, 321, 601, 623, 644, 665 |
| Cfr42I | CCGCGG | 1 cut(s) | 151 |
| CpoI | CGGWCCG | 1 cut(s) | 601 |
| CseI | GACGC | 1 cut(s) | 792 |
| Csp6I | GTAC | 1 cut(s) | 258 |
| CspI | CGGWCCG | 1 cut(s) | 601 |
| CviAII | CATG | 1 cut(s) | 825 |
| CviQI | GTAC | 1 cut(s) | 258 |
| DdeI | CTNAG | 3 cut(s) | 13, 358, 727 |
| DpnI | GATC | 3 cut(s) | 52, 61, 448 |
| DpnII | GATC | 3 cut(s) | 50, 59, 446 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 319 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 319 |
| Ecl136II | GAGCTC | 1 cut(s) | 357 |
| Eco130I | CCWWGG | 1 cut(s) | 512 |
| Eco147I | AGGCCT | 1 cut(s) | 511 |
| Eco24I | GRGCYC | 1 cut(s) | 359 |
| Eco47I | GGWCC | 2 cut(s) | 321, 601 |
| Eco53kI | GAGCTC | 1 cut(s) | 357 |
| Eco88I | CYCGRG | 1 cut(s) | 222 |
| EcoICRI | GAGCTC | 1 cut(s) | 357 |
| EcoO109I | RGGNCCY | 1 cut(s) | 321 |
| EcoRII | CCWGG | 2 cut(s) | 234, 275 |
| EcoT14I | CCWWGG | 1 cut(s) | 512 |
| EcoT38I | GRGCYC | 1 cut(s) | 359 |
| ErhI | CCWWGG | 1 cut(s) | 512 |
| FaeI | CATG | 1 cut(s) | 828 |
| FaiI | YATR | 4 cut(s) | 285, 422, 424, 826 |
| FaqI | GGGAC | 1 cut(s) | 585 |
| FatI | CATG | 1 cut(s) | 824 |
| FauI | CCCGC | 2 cut(s) | 226, 753 |
| Fnu4HI | GCNGC | 7 cut(s) | 148, 151, 184, 187, 190, 383, 775 |
| FokI | GGATG | 3 cut(s) | 259, 312, 813 |
| FriOI | GRGCYC | 1 cut(s) | 359 |
| Fsp4HI | GCNGC | 7 cut(s) | 148, 151, 184, 187, 190, 383, 775 |
| FspBI | CTAG | 5 cut(s) | 63, 339, 484, 693, 787 |
| GlaI | GCGC | 3 cut(s) | 344, 346, 478 |
| GluI | GCNGC | 7 cut(s) | 148, 151, 184, 187, 190, 383, 775 |
| GsaI | CCCAGC | 1 cut(s) | 124 |
| GsuI | CTGGAG | 1 cut(s) | 261 |
| HaeIII | GGCC | 7 cut(s) | 153, 405, 511, 624, 645, 666, 832 |
| HapII | CCGG | 1 cut(s) | 138 |
| HgaI | GACGC | 1 cut(s) | 792 |
| HhaI | GCGC | 3 cut(s) | 345, 347, 479 |
| Hin1II | CATG | 1 cut(s) | 828 |
| Hin6I | GCGC | 3 cut(s) | 343, 345, 477 |
| HinP1I | GCGC | 3 cut(s) | 343, 345, 477 |
| HincII | GTYRAC | 2 cut(s) | 197, 652 |
| HindII | GTYRAC | 2 cut(s) | 197, 652 |
| HinfI | GANTC | 2 cut(s) | 737, 821 |
| HpaII | CCGG | 1 cut(s) | 138 |
| HphI | GGTGA | 3 cut(s) | 389, 404, 671 |
| Hpy166II | GTNNAC | 3 cut(s) | 197, 412, 652 |
| Hpy188I | TCNGA | 4 cut(s) | 451, 601, 730, 759 |
| Hpy188III | TCNNGA | 9 cut(s) | 87, 108, 171, 324, 360, 444, 537, 612, 798 |
| Hpy8I | GTNNAC | 3 cut(s) | 197, 412, 652 |
| HpyAV | CCTTC | 2 cut(s) | 606, 722 |
| HpyCH4III | ACNGT | 1 cut(s) | 32 |
| HpyCH4V | TGCA | 2 cut(s) | 132, 828 |
| HpyF3I | CTNAG | 3 cut(s) | 13, 358, 727 |
| Hsp92II | CATG | 1 cut(s) | 828 |
| HspAI | GCGC | 3 cut(s) | 343, 345, 477 |
| KspI | CCGCGG | 1 cut(s) | 151 |
| Kzo9I | GATC | 3 cut(s) | 50, 59, 446 |
| LmnI | GCTCC | 6 cut(s) | 11, 44, 117, 140, 218, 767 |
| Lsp1109I | GCAGC | 3 cut(s) | 170, 176, 761 |
| LweI | GCATC | 1 cut(s) | 194 |
| MaeI | CTAG | 5 cut(s) | 63, 339, 484, 693, 787 |
| MaeIII | GTNAC | 2 cut(s) | 26, 659 |
| MalI | GATC | 3 cut(s) | 52, 61, 448 |
| MbiI | CCGCTC | 1 cut(s) | 221 |
| MboI | GATC | 3 cut(s) | 50, 59, 446 |
| MboII | GAAGA | 1 cut(s) | 432 |
| MfeI | CAATTG | 1 cut(s) | 627 |
| MhlI | GDGCHC | 1 cut(s) | 359 |
| MluCI | AATT | 2 cut(s) | 580, 627 |
| MlyI | GAGTC | 2 cut(s) | 731, 815 |
| MseI | TTAA | 1 cut(s) | 454 |
| MspA1I | CMGCKG | 1 cut(s) | 150 |
| MspI | CCGG | 1 cut(s) | 138 |
| MspR9I | CCNGG | 2 cut(s) | 236, 277 |
| MunI | CAATTG | 1 cut(s) | 627 |
| MvaI | CCWGG | 2 cut(s) | 236, 277 |
| MvnI | CGCG | 9 cut(s) | 150, 256, 345, 347, 385, 471, 477, 538, 748 |
| NdeII | GATC | 3 cut(s) | 50, 59, 446 |
| NlaIII | CATG | 1 cut(s) | 828 |
| NlaIV | GGNNCC | 4 cut(s) | 142, 154, 322, 685 |
| NmeAIII | GCCGAG | 1 cut(s) | 431 |
| NmuCI | GTSAC | 1 cut(s) | 659 |
| NruI | TCGCGA | 1 cut(s) | 538 |
| NspV | TTCGAA | 1 cut(s) | 715 |
| PaeR7I | CTCGAG | 1 cut(s) | 222 |
| PauI | GCGCGC | 1 cut(s) | 343 |
| PceI | AGGCCT | 1 cut(s) | 511 |
| PfoI | TCCNGGA | 1 cut(s) | 275 |
| PkrI | GCNGC | 7 cut(s) | 149, 152, 185, 188, 191, 384, 776 |
| Ple19I | CGATCG | 1 cut(s) | 449 |
| PleI | GAGTC | 2 cut(s) | 731, 815 |
| PpsI | GAGTC | 2 cut(s) | 731, 815 |
| PpuMI | RGGWCCY | 1 cut(s) | 321 |
| PshBI | ATTAAT | 1 cut(s) | 454 |
| Psp124BI | GAGCTC | 1 cut(s) | 359 |
| Psp5II | RGGWCCY | 1 cut(s) | 321 |
| Psp6I | CCWGG | 2 cut(s) | 234, 275 |
| PspFI | CCCAGC | 1 cut(s) | 120 |
| PspGI | CCWGG | 2 cut(s) | 234, 275 |
| PspN4I | GGNNCC | 4 cut(s) | 142, 154, 322, 685 |
| PspPI | GGNCC | 6 cut(s) | 152, 321, 601, 623, 644, 665 |
| PspPPI | RGGWCCY | 1 cut(s) | 321 |
| PspXI | VCTCGAGB | 1 cut(s) | 222 |
| PteI | GCGCGC | 1 cut(s) | 343 |
| PvuI | CGATCG | 1 cut(s) | 449 |
| RruI | TCGCGA | 1 cut(s) | 538 |
| RsaI | GTAC | 1 cut(s) | 259 |
| RsaNI | GTAC | 1 cut(s) | 258 |
| Rsr2I | CGGWCCG | 1 cut(s) | 601 |
| RsrII | CGGWCCG | 1 cut(s) | 601 |
| SacI | GAGCTC | 1 cut(s) | 359 |
| SacII | CCGCGG | 1 cut(s) | 151 |
| SaqAI | TTAA | 1 cut(s) | 454 |
| SatI | GCNGC | 7 cut(s) | 148, 151, 184, 187, 190, 383, 775 |
| Sau3AI | GATC | 3 cut(s) | 50, 59, 446 |
| Sau96I | GGNCC | 6 cut(s) | 152, 321, 601, 623, 644, 665 |
| SchI | GAGTC | 2 cut(s) | 731, 815 |
| ScrFI | CCNGG | 2 cut(s) | 236, 277 |
| SduI | GDGCHC | 1 cut(s) | 359 |
| SfaNI | GCATC | 1 cut(s) | 194 |
| SfcI | CTRYAG | 1 cut(s) | 306 |
| Sfr274I | CTCGAG | 1 cut(s) | 222 |
| Sfr303I | CCGCGG | 1 cut(s) | 151 |
| SfuI | TTCGAA | 1 cut(s) | 715 |
| SgrBI | CCGCGG | 1 cut(s) | 151 |
| SinI | GGWCC | 2 cut(s) | 321, 601 |
| SlaI | CTCGAG | 1 cut(s) | 222 |
| SmlI | CTYRAG | 5 cut(s) | 85, 169, 222, 672, 739 |
| SmoI | CTYRAG | 5 cut(s) | 85, 169, 222, 672, 739 |
| Sse9I | AATT | 2 cut(s) | 580, 627 |
| SseBI | AGGCCT | 1 cut(s) | 511 |
| SspI | AATATT | 1 cut(s) | 366 |
| SspMI | CTAG | 5 cut(s) | 63, 339, 484, 693, 787 |
| SstI | GAGCTC | 1 cut(s) | 359 |
| StuI | AGGCCT | 1 cut(s) | 511 |
| StyD4I | CCNGG | 2 cut(s) | 234, 275 |
| StyI | CCWWGG | 1 cut(s) | 512 |
| TaaI | ACNGT | 1 cut(s) | 32 |
| TaqI | TCGA | 6 cut(s) | 163, 223, 262, 506, 634, 715 |
| TaqII | GACCGA | 1 cut(s) | 618 |
| TasI | AATT | 2 cut(s) | 580, 627 |
| TauI | GCSGC | 4 cut(s) | 150, 153, 189, 385 |
| Tru1I | TTAA | 1 cut(s) | 454 |
| Tru9I | TTAA | 1 cut(s) | 454 |
| TscAI | CASTG | 1 cut(s) | 206 |
| TseFI | GTSAC | 1 cut(s) | 659 |
| TseI | GCWGC | 3 cut(s) | 183, 189, 774 |
| Tsp45I | GTSAC | 1 cut(s) | 659 |
| TspDTI | ATGAA | 3 cut(s) | 304, 472, 817 |
| TspRI | CASTG | 1 cut(s) | 206 |
| VpaK11BI | GGWCC | 2 cut(s) | 321, 601 |
| VspI | ATTAAT | 1 cut(s) | 454 |
| XhoI | CTCGAG | 1 cut(s) | 222 |
| XspI | CTAG | 5 cut(s) | 63, 339, 484, 693, 787 |