Plant mobile domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1598 |
| AatII | GACGTC | 1 cut(s) | 725 |
| Acc36I | ACCTGC | 1 cut(s) | 147 |
| AccB1I | GGYRCC | 1 cut(s) | 1891 |
| AccB7I | CCANNNNNTGG | 4 cut(s) | 201, 753, 1426, 1801 |
| AccI | GTMKAC | 1 cut(s) | 743 |
| AccIII | TCCGGA | 1 cut(s) | 1435 |
| AclWI | GGATC | 7 cut(s) | 666, 679, 681, 694, 979, 1734, 1900 |
| AcoI | YGGCCR | 5 cut(s) | 78, 567, 1268, 1428, 1868 |
| AcsI | RAATTY | 1 cut(s) | 496 |
| AcuI | CTGAAG | 2 cut(s) | 1352, 1619 |
| AcyI | GRCGYC | 4 cut(s) | 722, 1178, 1363, 1892 |
| AdeI | CACNNNGTG | 1 cut(s) | 201 |
| AfaI | GTAC | 8 cut(s) | 42, 534, 615, 784, 823, 1063, 1727, 1886 |
| AgeI | ACCGGT | 2 cut(s) | 591, 738 |
| AgsI | TTSAA | 3 cut(s) | 886, 1287, 1474 |
| AjnI | CCWGG | 6 cut(s) | 674, 900, 1069, 1365, 1371, 1931 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 683, 715 |
| AluBI | AGCT | 3 cut(s) | 54, 999, 1621 |
| AluI | AGCT | 3 cut(s) | 54, 999, 1621 |
| Alw21I | GWGCWC | 2 cut(s) | 406, 453 |
| Alw26I | GTCTC | 2 cut(s) | 55, 640 |
| AlwI | GGATC | 7 cut(s) | 666, 679, 681, 694, 979, 1734, 1900 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1426, 1642 |
| Aor13HI | TCCGGA | 1 cut(s) | 1435 |
| ApeKI | GCWGC | 4 cut(s) | 153, 222, 729, 1300 |
| ApoI | RAATTY | 1 cut(s) | 496 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1246, 1278 |
| AsiGI | ACCGGT | 2 cut(s) | 591, 738 |
| AsiSI | GCGATCGC | 1 cut(s) | 924 |
| AspLEI | GCGC | 8 cut(s) | 907, 928, 930, 932, 1535, 1677, 1788, 1894 |
| AspS9I | GGNCC | 9 cut(s) | 386, 458, 555, 661, 1369, 1524, 1545, 1607, 1668 |
| AsuC2I | CCSGG | 1 cut(s) | 96 |
| AsuHPI | GGTGA | 9 cut(s) | 281, 359, 365, 1464, 1503, 1657, 1816, 1927, 1938 |
| AvaII | GGWCC | 3 cut(s) | 458, 1545, 1607 |
| BalI | TGGCCA | 1 cut(s) | 1430 |
| BamHI | GGATCC | 2 cut(s) | 671, 686 |
| BanI | GGYRCC | 1 cut(s) | 1891 |
| BanII | GRGCYC | 3 cut(s) | 606, 1398, 1940 |
| BbsI | GAAGAC | 2 cut(s) | 1453, 1925 |
| Bbv12I | GWGCWC | 2 cut(s) | 406, 453 |
| BbvI | GCAGC | 4 cut(s) | 165, 209, 716, 1287 |
| BccI | CCATC | 8 cut(s) | 96, 318, 324, 333, 546, 1180, 1279, 1408 |
| BceAI | ACGGC | 4 cut(s) | 65, 582, 1165, 1855 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1300, 1334, 1728, 1762 |
| BciT130I | CCWGG | 6 cut(s) | 676, 902, 1071, 1367, 1373, 1933 |
| BciVI | GTATCC | 1 cut(s) | 1195 |
| BcnI | CCSGG | 1 cut(s) | 96 |
| BcoDI | GTCTC | 2 cut(s) | 55, 640 |
| BfaI | CTAG | 6 cut(s) | 44, 65, 583, 598, 650, 1774 |
| BfmI | CTRYAG | 2 cut(s) | 915, 1901 |
| BfoI | RGCGCY | 4 cut(s) | 908, 1678, 1789, 1895 |
| BfuAI | ACCTGC | 1 cut(s) | 147 |
| BfuI | GTATCC | 1 cut(s) | 1195 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 1031, 1666 |
| BglII | AGATCT | 1 cut(s) | 134 |
| BmcAI | AGTACT | 2 cut(s) | 42, 1886 |
| Bme1390I | CCNGG | 7 cut(s) | 96, 676, 902, 1071, 1367, 1373, 1933 |
| Bme18I | GGWCC | 3 cut(s) | 458, 1545, 1607 |
| BmgT120I | GGNCC | 9 cut(s) | 386, 458, 555, 661, 1369, 1524, 1545, 1607, 1668 |
| BmiI | GGNNCC | 9 cut(s) | 415, 603, 663, 673, 688, 1547, 1754, 1893, 1937 |
| BmrFI | CCNGG | 7 cut(s) | 96, 676, 902, 1071, 1367, 1373, 1933 |
| BmrI | ACTGGG | 1 cut(s) | 1738 |
| BmsI | GCATC | 5 cut(s) | 645, 1056, 1326, 1506, 1721 |
| BmuI | ACTGGG | 1 cut(s) | 1738 |
| BpiI | GAAGAC | 2 cut(s) | 1453, 1925 |
| BpmI | CTGGAG | 4 cut(s) | 1260, 1403, 1596, 1936 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1166 |
| BpuEI | CTTGAG | 1 cut(s) | 1799 |
| BpuMI | CCSGG | 1 cut(s) | 96 |
| Bsa29I | ATCGAT | 2 cut(s) | 175, 328 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1496, 1834 |
| BsaHI | GRCGYC | 4 cut(s) | 722, 1178, 1363, 1892 |
| BsaJI | CCNNGG | 7 cut(s) | 382, 422, 1181, 1371, 1520, 1561, 1679 |
| BsaWI | WCCGGW | 4 cut(s) | 591, 738, 1435, 1851 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 398, 428 |
| Bse118I | RCCGGY | 5 cut(s) | 460, 591, 738, 1122, 1604 |
| Bse1I | ACTGG | 8 cut(s) | 81, 534, 1148, 1431, 1431, 1598, 1744, 1909 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1496, 1834 |
| BseAI | TCCGGA | 1 cut(s) | 1435 |
| BseBI | CCWGG | 6 cut(s) | 676, 902, 1071, 1367, 1373, 1933 |
| BseCI | ATCGAT | 2 cut(s) | 175, 328 |
| BseDI | CCNNGG | 7 cut(s) | 382, 422, 1181, 1371, 1520, 1561, 1679 |
| BseGI | GGATG | 5 cut(s) | 636, 837, 1191, 1257, 1767 |
| BseJI | GATNNNNATC | 2 cut(s) | 1496, 1834 |
| BseMII | CTCAG | 3 cut(s) | 69, 1184, 1372 |
| BseNI | ACTGG | 8 cut(s) | 81, 534, 1148, 1431, 1431, 1598, 1744, 1909 |
| BsePI | GCGCGC | 2 cut(s) | 926, 928 |
| BseRI | GAGGAG | 2 cut(s) | 782, 1346 |
| BseX3I | CGGCCG | 2 cut(s) | 567, 1868 |
| BseXI | GCAGC | 4 cut(s) | 165, 209, 716, 1287 |
| BseYI | CCCAGC | 1 cut(s) | 1621 |
| BsgI | GTGCAG | 2 cut(s) | 492, 1768 |
| Bsh1285I | CGRYCG | 4 cut(s) | 570, 743, 924, 1871 |
| BshNI | GGYRCC | 1 cut(s) | 1891 |
| BshTI | ACCGGT | 2 cut(s) | 591, 738 |
| BshVI | ATCGAT | 2 cut(s) | 175, 328 |
| BsiEI | CGRYCG | 4 cut(s) | 570, 743, 924, 1871 |
| BsiHKAI | GWGCWC | 2 cut(s) | 406, 453 |
| BsiSI | CCGG | 8 cut(s) | 96, 461, 592, 739, 1123, 1436, 1605, 1852 |
| BslFI | GGGAC | 2 cut(s) | 709, 1203 |
| BsmAI | GTCTC | 2 cut(s) | 55, 640 |
| BsmFI | GGGAC | 2 cut(s) | 709, 1203 |
| Bsp1286I | GDGCHC | 5 cut(s) | 406, 453, 606, 1398, 1940 |
| Bsp13I | TCCGGA | 1 cut(s) | 1435 |
| Bsp19I | CCATGG | 2 cut(s) | 382, 1561 |
| Bsp68I | TCGCGA | 3 cut(s) | 118, 1392, 1717 |
| BspCNI | CTCAG | 3 cut(s) | 68, 1185, 1371 |
| BspDI | ATCGAT | 2 cut(s) | 175, 328 |
| BspEI | TCCGGA | 1 cut(s) | 1435 |
| BspLI | GGNNCC | 9 cut(s) | 415, 603, 663, 673, 688, 1547, 1754, 1893, 1937 |
| BspMAI | CTGCAG | 1 cut(s) | 1905 |
| BspMI | ACCTGC | 1 cut(s) | 147 |
| BspPI | GGATC | 7 cut(s) | 666, 679, 681, 694, 979, 1734, 1900 |
| BspQI | GCTCTTC | 1 cut(s) | 1638 |
| BspT107I | GGYRCC | 1 cut(s) | 1891 |
| BsrFI | RCCGGY | 5 cut(s) | 460, 591, 738, 1122, 1604 |
| BsrI | ACTGG | 8 cut(s) | 81, 534, 1148, 1431, 1431, 1598, 1744, 1909 |
| BssAI | RCCGGY | 5 cut(s) | 460, 591, 738, 1122, 1604 |
| BssECI | CCNNGG | 7 cut(s) | 382, 422, 1181, 1371, 1520, 1561, 1679 |
| BssHII | GCGCGC | 2 cut(s) | 926, 928 |
| BssNI | GRCGYC | 4 cut(s) | 722, 1178, 1363, 1892 |
| BssT1I | CCWWGG | 3 cut(s) | 382, 1520, 1561 |
| Bst2UI | CCWGG | 6 cut(s) | 676, 902, 1071, 1367, 1373, 1933 |
| Bst4CI | ACNGT | 5 cut(s) | 410, 426, 443, 1452, 1509 |
| Bst6I | CTCTTC | 4 cut(s) | 548, 1267, 1488, 1638 |
| BstACI | GRCGYC | 4 cut(s) | 722, 1178, 1363, 1892 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 594 |
| BstDEI | CTNAG | 5 cut(s) | 55, 988, 1166, 1193, 1358 |
| BstDSI | CCRYGG | 5 cut(s) | 382, 422, 1181, 1561, 1679 |
| BstEII | GGTNACC | 1 cut(s) | 1509 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 519 |
| BstF5I | GGATG | 5 cut(s) | 636, 837, 1191, 1257, 1767 |
| BstH2I | RGCGCY | 4 cut(s) | 908, 1678, 1789, 1895 |
| BstHHI | GCGC | 8 cut(s) | 907, 928, 930, 932, 1535, 1677, 1788, 1894 |
| BstMAI | GTCTC | 2 cut(s) | 55, 640 |
| BstMCI | CGRYCG | 4 cut(s) | 570, 743, 924, 1871 |
| BstNI | CCWGG | 6 cut(s) | 676, 902, 1071, 1367, 1373, 1933 |
| BstNSI | RCATGY | 1 cut(s) | 809 |
| BstPI | GGTNACC | 1 cut(s) | 1509 |
| BstSCI | CCNGG | 7 cut(s) | 94, 674, 900, 1069, 1365, 1371, 1931 |
| BstSFI | CTRYAG | 2 cut(s) | 915, 1901 |
| BstV1I | GCAGC | 4 cut(s) | 165, 209, 716, 1287 |
| BstV2I | GAAGAC | 2 cut(s) | 1453, 1925 |
| BstX2I | RGATCY | 4 cut(s) | 134, 671, 686, 1905 |
| BstYI | RGATCY | 4 cut(s) | 134, 671, 686, 1905 |
| BstZI | CGGCCG | 2 cut(s) | 567, 1868 |
| Bsu15I | ATCGAT | 2 cut(s) | 175, 328 |
| BsuI | GTATCC | 1 cut(s) | 1195 |
| BsuTUI | ATCGAT | 2 cut(s) | 175, 328 |
| BtgI | CCRYGG | 5 cut(s) | 382, 422, 1181, 1561, 1679 |
| BtgZI | GCGATG | 2 cut(s) | 565, 1698 |
| BtsCI | GGATG | 5 cut(s) | 636, 837, 1191, 1257, 1767 |
| BtsI | GCAGTG | 2 cut(s) | 406, 579 |
| BtsIMutI | CAGTG | 7 cut(s) | 74, 406, 415, 579, 1141, 1424, 1751 |
| BtuMI | TCGCGA | 3 cut(s) | 118, 1392, 1717 |
| BveI | ACCTGC | 1 cut(s) | 147 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1426, 1642 |
| CfoI | GCGC | 8 cut(s) | 907, 928, 930, 932, 1535, 1677, 1788, 1894 |
| Cfr10I | RCCGGY | 5 cut(s) | 460, 591, 738, 1122, 1604 |
| Cfr13I | GGNCC | 9 cut(s) | 386, 458, 555, 661, 1369, 1524, 1545, 1607, 1668 |
| ClaI | ATCGAT | 2 cut(s) | 175, 328 |
| CseI | GACGC | 3 cut(s) | 137, 1186, 1371 |
| Csp6I | GTAC | 8 cut(s) | 41, 533, 614, 783, 822, 1062, 1726, 1885 |
| CspAI | ACCGGT | 2 cut(s) | 591, 738 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 411, 446 |
| CviAII | CATG | 7 cut(s) | 348, 383, 806, 1465, 1562, 1923, 1947 |
| CviQI | GTAC | 8 cut(s) | 41, 533, 614, 783, 822, 1062, 1726, 1885 |
| DdeI | CTNAG | 5 cut(s) | 55, 988, 1166, 1193, 1358 |
| DinI | GGCGCC | 1 cut(s) | 1893 |
| DraIII | CACNNNGTG | 1 cut(s) | 201 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1598 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1598 |
| EaeI | YGGCCR | 5 cut(s) | 78, 567, 1268, 1428, 1868 |
| EagI | CGGCCG | 2 cut(s) | 567, 1868 |
| Eam1104I | CTCTTC | 4 cut(s) | 548, 1267, 1488, 1638 |
| EarI | CTCTTC | 4 cut(s) | 548, 1267, 1488, 1638 |
| EciI | GGCGGA | 1 cut(s) | 479 |
| EclXI | CGGCCG | 2 cut(s) | 567, 1868 |
| Eco130I | CCWWGG | 3 cut(s) | 382, 1520, 1561 |
| Eco147I | AGGCCT | 1 cut(s) | 525 |
| Eco24I | GRGCYC | 3 cut(s) | 606, 1398, 1940 |
| Eco47I | GGWCC | 3 cut(s) | 458, 1545, 1607 |
| Eco52I | CGGCCG | 2 cut(s) | 567, 1868 |
| Eco57I | CTGAAG | 2 cut(s) | 1352, 1619 |
| Eco91I | GGTNACC | 1 cut(s) | 1509 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 519 |
| EcoO65I | GGTNACC | 1 cut(s) | 1509 |
| EcoRII | CCWGG | 6 cut(s) | 674, 900, 1069, 1365, 1371, 1931 |
| EcoT14I | CCWWGG | 3 cut(s) | 382, 1520, 1561 |
| EcoT22I | ATGCAT | 1 cut(s) | 638 |
| EcoT38I | GRGCYC | 3 cut(s) | 606, 1398, 1940 |
| EgeI | GGCGCC | 1 cut(s) | 1893 |
| EheI | GGCGCC | 1 cut(s) | 1893 |
| ErhI | CCWWGG | 3 cut(s) | 382, 1520, 1561 |
| FaeI | CATG | 7 cut(s) | 351, 386, 809, 1468, 1565, 1926, 1950 |
| FaqI | GGGAC | 2 cut(s) | 709, 1203 |
| FatI | CATG | 7 cut(s) | 347, 382, 805, 1464, 1561, 1922, 1946 |
| FauI | CCCGC | 4 cut(s) | 734, 954, 1241, 1947 |
| FblI | GTMKAC | 1 cut(s) | 743 |
| FokI | GGATG | 5 cut(s) | 623, 844, 1198, 1264, 1754 |
| FriOI | GRGCYC | 3 cut(s) | 606, 1398, 1940 |
| FspBI | CTAG | 6 cut(s) | 44, 65, 583, 598, 650, 1774 |
| GlaI | GCGC | 8 cut(s) | 906, 927, 929, 931, 1534, 1676, 1787, 1893 |
| GsaI | CCCAGC | 1 cut(s) | 1625 |
| GsuI | CTGGAG | 4 cut(s) | 1260, 1403, 1596, 1936 |
| HaeII | RGCGCY | 4 cut(s) | 908, 1678, 1789, 1895 |
| HapII | CCGG | 8 cut(s) | 96, 461, 592, 739, 1123, 1436, 1605, 1852 |
| HgaI | GACGC | 3 cut(s) | 137, 1186, 1371 |
| HhaI | GCGC | 8 cut(s) | 907, 928, 930, 932, 1535, 1677, 1788, 1894 |
| Hin1I | GRCGYC | 4 cut(s) | 722, 1178, 1363, 1892 |
| Hin1II | CATG | 7 cut(s) | 351, 386, 809, 1468, 1565, 1926, 1950 |
| Hin6I | GCGC | 8 cut(s) | 905, 926, 928, 930, 1533, 1675, 1786, 1892 |
| HinP1I | GCGC | 8 cut(s) | 905, 926, 928, 930, 1533, 1675, 1786, 1892 |
| HincII | GTYRAC | 1 cut(s) | 744 |
| HindII | GTYRAC | 1 cut(s) | 744 |
| HinfI | GANTC | 8 cut(s) | 360, 540, 869, 893, 1097, 1242, 1477, 1684 |
| HpaII | CCGG | 8 cut(s) | 96, 461, 592, 739, 1123, 1436, 1605, 1852 |
| HphI | GGTGA | 9 cut(s) | 281, 359, 365, 1464, 1503, 1657, 1816, 1927, 1938 |
| Hpy166II | GTNNAC | 2 cut(s) | 744, 1929 |
| Hpy188I | TCNGA | 8 cut(s) | 58, 963, 1113, 1332, 1361, 1405, 1488, 1545 |
| Hpy8I | GTNNAC | 2 cut(s) | 744, 1929 |
| Hpy99I | CGWCG | 6 cut(s) | 716, 1180, 1492, 1604, 1726, 1876 |
| HpyAV | CCTTC | 9 cut(s) | 380, 835, 907, 1118, 1192, 1621, 1642, 1849, 1959 |
| HpyCH4III | ACNGT | 5 cut(s) | 410, 426, 443, 1452, 1509 |
| HpyCH4IV | ACGT | 2 cut(s) | 722, 1724 |
| HpyCH4V | TGCA | 5 cut(s) | 509, 521, 636, 1749, 1903 |
| HpyF3I | CTNAG | 5 cut(s) | 55, 988, 1166, 1193, 1358 |
| HpySE526I | ACGT | 2 cut(s) | 722, 1724 |
| Hsp92I | GRCGYC | 4 cut(s) | 722, 1178, 1363, 1892 |
| Hsp92II | CATG | 7 cut(s) | 351, 386, 809, 1468, 1565, 1926, 1950 |
| HspAI | GCGC | 8 cut(s) | 905, 926, 928, 930, 1533, 1675, 1786, 1892 |
| KasI | GGCGCC | 1 cut(s) | 1891 |
| Kpn2I | TCCGGA | 1 cut(s) | 1435 |
| KroI | GCCGGC | 1 cut(s) | 1122 |
| KroNI | GCCGGC | 1 cut(s) | 1124 |
| LguI | GCTCTTC | 1 cut(s) | 1638 |
| LmnI | GCTCC | 7 cut(s) | 419, 448, 601, 1011, 1461, 1935, 1955 |
| Lsp1109I | GCAGC | 4 cut(s) | 165, 209, 716, 1287 |
| LweI | GCATC | 5 cut(s) | 645, 1056, 1326, 1506, 1721 |
| MaeI | CTAG | 6 cut(s) | 44, 65, 583, 598, 650, 1774 |
| MaeII | ACGT | 2 cut(s) | 722, 1724 |
| MaeIII | GTNAC | 3 cut(s) | 365, 991, 1509 |
| MauBI | CGCGCGCG | 1 cut(s) | 926 |
| MfeI | CAATTG | 1 cut(s) | 1233 |
| MflI | RGATCY | 4 cut(s) | 134, 671, 686, 1905 |
| MhlI | GDGCHC | 5 cut(s) | 406, 453, 606, 1398, 1940 |
| MlsI | TGGCCA | 1 cut(s) | 1430 |
| MluNI | TGGCCA | 1 cut(s) | 1430 |
| Mly113I | GGCGCC | 1 cut(s) | 1892 |
| MlyI | GAGTC | 3 cut(s) | 354, 534, 1251 |
| MmeI | TCCRAC | 1 cut(s) | 1511 |
| Mox20I | TGGCCA | 1 cut(s) | 1430 |
| Mph1103I | ATGCAT | 1 cut(s) | 638 |
| MroI | TCCGGA | 1 cut(s) | 1435 |
| MroNI | GCCGGC | 1 cut(s) | 1122 |
| MscI | TGGCCA | 1 cut(s) | 1430 |
| Msp20I | TGGCCA | 1 cut(s) | 1430 |
| MspA1I | CMGCKG | 3 cut(s) | 729, 1248, 1642 |
| MspI | CCGG | 8 cut(s) | 96, 461, 592, 739, 1123, 1436, 1605, 1852 |
| MspR9I | CCNGG | 7 cut(s) | 96, 676, 902, 1071, 1367, 1373, 1933 |
| MunI | CAATTG | 1 cut(s) | 1233 |
| MvaI | CCWGG | 6 cut(s) | 676, 902, 1071, 1367, 1373, 1933 |
| NaeI | GCCGGC | 1 cut(s) | 1124 |
| NarI | GGCGCC | 1 cut(s) | 1892 |
| NciI | CCSGG | 1 cut(s) | 96 |
| NcoI | CCATGG | 2 cut(s) | 382, 1561 |
| NgoMIV | GCCGGC | 1 cut(s) | 1122 |
| NlaIII | CATG | 7 cut(s) | 351, 386, 809, 1468, 1565, 1926, 1950 |
| NlaIV | GGNNCC | 9 cut(s) | 415, 603, 663, 673, 688, 1547, 1754, 1893, 1937 |
| NmuCI | GTSAC | 2 cut(s) | 365, 1509 |
| NruI | TCGCGA | 3 cut(s) | 118, 1392, 1717 |
| NsiI | ATGCAT | 1 cut(s) | 638 |
| NspI | RCATGY | 1 cut(s) | 809 |
| PauI | GCGCGC | 2 cut(s) | 926, 928 |
| PceI | AGGCCT | 1 cut(s) | 525 |
| PciSI | GCTCTTC | 1 cut(s) | 1638 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 1389, 1596, 1721 |
| PdiI | GCCGGC | 1 cut(s) | 1124 |
| PfeI | GAWTC | 5 cut(s) | 869, 893, 1097, 1477, 1684 |
| PflFI | GACNNNGTC | 1 cut(s) | 363 |
| PflMI | CCANNNNNTGG | 4 cut(s) | 201, 753, 1426, 1801 |
| PfoI | TCCNGGA | 1 cut(s) | 674 |
| PinAI | ACCGGT | 2 cut(s) | 591, 738 |
| Ple19I | CGATCG | 1 cut(s) | 924 |
| PleI | GAGTC | 3 cut(s) | 354, 534, 1250 |
| PluTI | GGCGCC | 1 cut(s) | 1895 |
| PpsI | GAGTC | 3 cut(s) | 354, 534, 1250 |
| Psp6I | CCWGG | 6 cut(s) | 674, 900, 1069, 1365, 1371, 1931 |
| PspEI | GGTNACC | 1 cut(s) | 1509 |
| PspFI | CCCAGC | 1 cut(s) | 1621 |
| PspGI | CCWGG | 6 cut(s) | 674, 900, 1069, 1365, 1371, 1931 |
| PspN4I | GGNNCC | 9 cut(s) | 415, 603, 663, 673, 688, 1547, 1754, 1893, 1937 |
| PspPI | GGNCC | 9 cut(s) | 386, 458, 555, 661, 1369, 1524, 1545, 1607, 1668 |
| PstI | CTGCAG | 1 cut(s) | 1905 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1426, 1642 |
| PsuI | RGATCY | 4 cut(s) | 134, 671, 686, 1905 |
| PsyI | GACNNNGTC | 1 cut(s) | 363 |
| PteI | GCGCGC | 2 cut(s) | 926, 928 |
| PvuI | CGATCG | 1 cut(s) | 924 |
| RgaI | GCGATCGC | 1 cut(s) | 924 |
| RruI | TCGCGA | 3 cut(s) | 118, 1392, 1717 |
| RsaI | GTAC | 8 cut(s) | 42, 534, 615, 784, 823, 1063, 1727, 1886 |
| RsaNI | GTAC | 8 cut(s) | 41, 533, 614, 783, 822, 1062, 1726, 1885 |
| SalI | GTCGAC | 1 cut(s) | 742 |
| SapI | GCTCTTC | 1 cut(s) | 1638 |
| Sau96I | GGNCC | 9 cut(s) | 386, 458, 555, 661, 1369, 1524, 1545, 1607, 1668 |
| ScaI | AGTACT | 2 cut(s) | 42, 1886 |
| SchI | GAGTC | 3 cut(s) | 354, 534, 1251 |
| ScrFI | CCNGG | 7 cut(s) | 96, 676, 902, 1071, 1367, 1373, 1933 |
| SduI | GDGCHC | 5 cut(s) | 406, 453, 606, 1398, 1940 |
| SfaAI | GCGATCGC | 1 cut(s) | 924 |
| SfaNI | GCATC | 5 cut(s) | 645, 1056, 1326, 1506, 1721 |
| SfcI | CTRYAG | 2 cut(s) | 915, 1901 |
| SfoI | GGCGCC | 1 cut(s) | 1893 |
| SgfI | GCGATCGC | 1 cut(s) | 924 |
| SinI | GGWCC | 3 cut(s) | 458, 1545, 1607 |
| SmlI | CTYRAG | 1 cut(s) | 1814 |
| SmoI | CTYRAG | 1 cut(s) | 1814 |
| SseBI | AGGCCT | 1 cut(s) | 525 |
| SspDI | GGCGCC | 1 cut(s) | 1891 |
| SspMI | CTAG | 6 cut(s) | 44, 65, 583, 598, 650, 1774 |
| StuI | AGGCCT | 1 cut(s) | 525 |
| StyD4I | CCNGG | 7 cut(s) | 94, 674, 900, 1069, 1365, 1371, 1931 |
| StyI | CCWWGG | 3 cut(s) | 382, 1520, 1561 |
| TaaI | ACNGT | 5 cut(s) | 410, 426, 443, 1452, 1509 |
| TaiI | ACGT | 2 cut(s) | 725, 1727 |
| TaqI | TCGA | 9 cut(s) | 175, 328, 378, 714, 743, 1175, 1310, 1551, 1590 |
| TatI | WGTACW | 2 cut(s) | 40, 1884 |
| TauI | GCSGC | 8 cut(s) | 572, 773, 1270, 1342, 1642, 1765, 1813, 1870 |
| TfiI | GAWTC | 5 cut(s) | 869, 893, 1097, 1477, 1684 |
| TscAI | CASTG | 7 cut(s) | 81, 406, 415, 579, 1148, 1431, 1751 |
| TseFI | GTSAC | 2 cut(s) | 365, 1509 |
| TseI | GCWGC | 4 cut(s) | 153, 222, 729, 1300 |
| Tsp45I | GTSAC | 2 cut(s) | 365, 1509 |
| TspDTI | ATGAA | 4 cut(s) | 177, 489, 1481, 1589 |
| TspGWI | ACGGA | 4 cut(s) | 171, 905, 1218, 1696 |
| TspRI | CASTG | 7 cut(s) | 81, 406, 415, 579, 1148, 1431, 1751 |
| Tth111I | GACNNNGTC | 1 cut(s) | 363 |
| Van91I | CCANNNNNTGG | 4 cut(s) | 201, 753, 1426, 1801 |
| VpaK11BI | GGWCC | 3 cut(s) | 458, 1545, 1607 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 519 |
| XapI | RAATTY | 1 cut(s) | 496 |
| XceI | RCATGY | 1 cut(s) | 809 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1373 |
| XmiI | GTMKAC | 1 cut(s) | 743 |
| XspI | CTAG | 6 cut(s) | 44, 65, 583, 598, 650, 1774 |
| ZraI | GACGTC | 1 cut(s) | 723 |
| ZrmI | AGTACT | 2 cut(s) | 42, 1886 |
| Zsp2I | ATGCAT | 1 cut(s) | 638 |