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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 518 |
| AccB1I | GGYRCC | 1 cut(s) | 638 |
| AccBSI | CCGCTC | 2 cut(s) | 701, 1007 |
| AccII | CGCG | 6 cut(s) | 130, 157, 159, 761, 982, 1030 |
| AclWI | GGATC | 1 cut(s) | 15 |
| AcoI | YGGCCR | 2 cut(s) | 949, 997 |
| AcyI | GRCGYC | 1 cut(s) | 843 |
| AfaI | GTAC | 1 cut(s) | 189 |
| AfeI | AGCGCT | 1 cut(s) | 359 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 254, 413, 493, 863, 1006 |
| AflIII | ACRYGT | 2 cut(s) | 829, 1028 |
| AgeI | ACCGGT | 1 cut(s) | 57 |
| AgsI | TTSAA | 2 cut(s) | 102, 141 |
| AjiI | CACGTC | 1 cut(s) | 563 |
| AjnI | CCWGG | 1 cut(s) | 856 |
| AluBI | AGCT | 5 cut(s) | 606, 656, 793, 872, 989 |
| AluI | AGCT | 5 cut(s) | 606, 656, 793, 872, 989 |
| Alw21I | GWGCWC | 1 cut(s) | 874 |
| Alw26I | GTCTC | 6 cut(s) | 174, 225, 432, 897, 917, 1037 |
| AlwI | GGATC | 1 cut(s) | 15 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 857 |
| Ama87I | CYCGRG | 1 cut(s) | 228 |
| Aor51HI | AGCGCT | 1 cut(s) | 359 |
| AoxI | GGCC | 8 cut(s) | 205, 328, 404, 577, 595, 743, 949, 997 |
| ApaI | GGGCCC | 1 cut(s) | 581 |
| ApeKI | GCWGC | 4 cut(s) | 269, 360, 371, 851 |
| AsiGI | ACCGGT | 1 cut(s) | 57 |
| AspLEI | GCGC | 4 cut(s) | 159, 360, 509, 763 |
| AspS9I | GGNCC | 8 cut(s) | 152, 206, 329, 405, 484, 577, 578, 596 |
| AsuC2I | CCSGG | 2 cut(s) | 112, 494 |
| AsuHPI | GGTGA | 5 cut(s) | 233, 273, 309, 482, 708 |
| AvaI | CYCGRG | 1 cut(s) | 228 |
| AvaII | GGWCC | 2 cut(s) | 152, 484 |
| BaeGI | GKGCMC | 2 cut(s) | 581, 643 |
| BalI | TGGCCA | 2 cut(s) | 951, 999 |
| BanI | GGYRCC | 1 cut(s) | 638 |
| BanII | GRGCYC | 2 cut(s) | 581, 874 |
| Bbv12I | GWGCWC | 1 cut(s) | 874 |
| BbvI | GCAGC | 4 cut(s) | 256, 347, 383, 863 |
| BccI | CCATC | 5 cut(s) | 297, 298, 687, 724, 1008 |
| BceAI | ACGGC | 3 cut(s) | 99, 449, 630 |
| BciT130I | CCWGG | 1 cut(s) | 858 |
| BcnI | CCSGG | 2 cut(s) | 112, 494 |
| BcoDI | GTCTC | 6 cut(s) | 174, 225, 432, 897, 917, 1037 |
| BfaI | CTAG | 1 cut(s) | 747 |
| BfmI | CTRYAG | 1 cut(s) | 361 |
| BfoI | RGCGCY | 2 cut(s) | 361, 510 |
| BfuAI | ACCTGC | 1 cut(s) | 518 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 336 |
| BisI | GCNGC | 9 cut(s) | 205, 270, 361, 372, 490, 679, 743, 852, 945 |
| BlsI | GCNGC | 9 cut(s) | 206, 271, 362, 373, 491, 680, 744, 853, 946 |
| Bme1390I | CCNGG | 3 cut(s) | 112, 494, 858 |
| Bme18I | GGWCC | 2 cut(s) | 152, 484 |
| BmeT110I | CYCGRG | 1 cut(s) | 228 |
| BmgBI | CACGTC | 1 cut(s) | 563 |
| BmgT120I | GGNCC | 8 cut(s) | 152, 206, 329, 405, 484, 577, 578, 596 |
| BmiI | GGNNCC | 7 cut(s) | 27, 259, 463, 498, 578, 579, 640 |
| BmrFI | CCNGG | 3 cut(s) | 112, 494, 858 |
| BmsI | GCATC | 1 cut(s) | 376 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 645, 677 |
| BpmI | CTGGAG | 3 cut(s) | 198, 531, 879 |
| Bpu10I | CCTNAGC | 1 cut(s) | 873 |
| BpuMI | CCSGG | 2 cut(s) | 112, 494 |
| BsaHI | GRCGYC | 1 cut(s) | 843 |
| BsaI | GGTCTC | 2 cut(s) | 174, 897 |
| BsaJI | CCNNGG | 8 cut(s) | 193, 253, 544, 766, 856, 887, 952, 980 |
| BsaWI | WCCGGW | 2 cut(s) | 57, 260 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 171, 201, 450, 480 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 254, 413, 493, 863, 1006 |
| Bse118I | RCCGGY | 3 cut(s) | 57, 311, 431 |
| Bse1I | ACTGG | 4 cut(s) | 76, 241, 248, 931 |
| BseBI | CCWGG | 1 cut(s) | 858 |
| BseDI | CCNNGG | 8 cut(s) | 193, 253, 544, 766, 856, 887, 952, 980 |
| BseGI | GGATG | 2 cut(s) | 308, 481 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 254, 413, 493, 863, 1006 |
| BseMII | CTCAG | 2 cut(s) | 234, 887 |
| BseNI | ACTGG | 4 cut(s) | 76, 241, 248, 931 |
| BseRI | GAGGAG | 1 cut(s) | 474 |
| BseSI | GKGCMC | 2 cut(s) | 581, 643 |
| BseXI | GCAGC | 4 cut(s) | 256, 347, 383, 863 |
| BsgI | GTGCAG | 1 cut(s) | 339 |
| Bsh1236I | CGCG | 6 cut(s) | 130, 157, 159, 761, 982, 1030 |
| BshFI | GGCC | 8 cut(s) | 207, 330, 406, 579, 597, 745, 951, 999 |
| BshNI | GGYRCC | 1 cut(s) | 638 |
| BshTI | ACCGGT | 1 cut(s) | 57 |
| BsiHKAI | GWGCWC | 1 cut(s) | 874 |
| BsiHKCI | CYCGRG | 1 cut(s) | 228 |
| BsiSI | CCGG | 6 cut(s) | 58, 111, 261, 312, 432, 493 |
| BslFI | GGGAC | 1 cut(s) | 1023 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 254, 413, 493, 863, 1006 |
| BsmAI | GTCTC | 6 cut(s) | 174, 225, 432, 897, 917, 1037 |
| BsmBI | CGTCTC | 1 cut(s) | 1037 |
| BsmFI | GGGAC | 1 cut(s) | 1023 |
| BsnI | GGCC | 8 cut(s) | 207, 330, 406, 579, 597, 745, 951, 999 |
| Bso31I | GGTCTC | 2 cut(s) | 174, 897 |
| BsoBI | CYCGRG | 1 cut(s) | 228 |
| Bsp120I | GGGCCC | 1 cut(s) | 577 |
| Bsp1286I | GDGCHC | 3 cut(s) | 581, 643, 874 |
| Bsp1407I | TGTACA | 1 cut(s) | 187 |
| Bsp143I | GATC | 2 cut(s) | 7, 938 |
| Bsp68I | TCGCGA | 1 cut(s) | 130 |
| BspANI | GGCC | 8 cut(s) | 207, 330, 406, 579, 597, 745, 951, 999 |
| BspCNI | CTCAG | 2 cut(s) | 235, 886 |
| BspFNI | CGCG | 6 cut(s) | 130, 157, 159, 761, 982, 1030 |
| BspHI | TCATGA | 1 cut(s) | 685 |
| BspLI | GGNNCC | 7 cut(s) | 27, 259, 463, 498, 578, 579, 640 |
| BspMAI | CTGCAG | 1 cut(s) | 365 |
| BspMI | ACCTGC | 1 cut(s) | 518 |
| BspPI | GGATC | 1 cut(s) | 15 |
| BspT107I | GGYRCC | 1 cut(s) | 638 |
| BspTNI | GGTCTC | 2 cut(s) | 174, 897 |
| BsrBI | CCGCTC | 2 cut(s) | 701, 1007 |
| BsrFI | RCCGGY | 3 cut(s) | 57, 311, 431 |
| BsrGI | TGTACA | 1 cut(s) | 187 |
| BsrI | ACTGG | 4 cut(s) | 76, 241, 248, 931 |
| BssAI | RCCGGY | 3 cut(s) | 57, 311, 431 |
| BssECI | CCNNGG | 8 cut(s) | 193, 253, 544, 766, 856, 887, 952, 980 |
| BssMI | GATC | 2 cut(s) | 7, 938 |
| BssNI | GRCGYC | 1 cut(s) | 843 |
| BssT1I | CCWWGG | 2 cut(s) | 887, 952 |
| Bst2UI | CCWGG | 1 cut(s) | 858 |
| Bst4CI | ACNGT | 3 cut(s) | 443, 620, 730 |
| BstACI | GRCGYC | 1 cut(s) | 843 |
| BstAUI | TGTACA | 1 cut(s) | 187 |
| BstC8I | GCNNGC | 7 cut(s) | 21, 202, 322, 391, 581, 643, 949 |
| BstDEI | CTNAG | 2 cut(s) | 243, 873 |
| BstDSI | CCRYGG | 2 cut(s) | 766, 980 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 861 |
| BstF5I | GGATG | 2 cut(s) | 308, 481 |
| BstFNI | CGCG | 6 cut(s) | 130, 157, 159, 761, 982, 1030 |
| BstH2I | RGCGCY | 2 cut(s) | 361, 510 |
| BstHHI | GCGC | 4 cut(s) | 159, 360, 509, 763 |
| BstKTI | GATC | 2 cut(s) | 10, 941 |
| BstMAI | GTCTC | 6 cut(s) | 174, 225, 432, 897, 917, 1037 |
| BstMBI | GATC | 2 cut(s) | 7, 938 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 275, 317, 336, 380, 603, 675 |
| BstNI | CCWGG | 1 cut(s) | 858 |
| BstNSI | RCATGY | 1 cut(s) | 833 |
| BstSCI | CCNGG | 3 cut(s) | 110, 492, 856 |
| BstSFI | CTRYAG | 1 cut(s) | 361 |
| BstSLI | GKGCMC | 2 cut(s) | 581, 643 |
| BstUI | CGCG | 6 cut(s) | 130, 157, 159, 761, 982, 1030 |
| BstV1I | GCAGC | 4 cut(s) | 256, 347, 383, 863 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 339 |
| BsuRI | GGCC | 8 cut(s) | 207, 330, 406, 579, 597, 745, 951, 999 |
| BtgI | CCRYGG | 2 cut(s) | 766, 980 |
| BtrI | CACGTC | 1 cut(s) | 563 |
| BtsCI | GGATG | 2 cut(s) | 308, 481 |
| BtsI | GCAGTG | 1 cut(s) | 154 |
| BtsIMutI | CAGTG | 3 cut(s) | 154, 234, 240 |
| BtuMI | TCGCGA | 1 cut(s) | 130 |
| BveI | ACCTGC | 1 cut(s) | 518 |
| Cac8I | GCNNGC | 7 cut(s) | 21, 202, 322, 391, 581, 643, 949 |
| CaiI | CAGNNNCTG | 1 cut(s) | 857 |
| CciI | TCATGA | 1 cut(s) | 685 |
| CfoI | GCGC | 4 cut(s) | 159, 360, 509, 763 |
| Cfr10I | RCCGGY | 3 cut(s) | 57, 311, 431 |
| Cfr13I | GGNCC | 8 cut(s) | 152, 206, 329, 405, 484, 577, 578, 596 |
| Cfr42I | CCGCGG | 1 cut(s) | 983 |
| CseI | GACGC | 3 cut(s) | 141, 832, 1019 |
| Csp6I | GTAC | 1 cut(s) | 188 |
| CspAI | ACCGGT | 1 cut(s) | 57 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 383, 418, 755, 790 |
| CviAII | CATG | 3 cut(s) | 185, 686, 830 |
| CviQI | GTAC | 1 cut(s) | 188 |
| DdeI | CTNAG | 2 cut(s) | 243, 873 |
| DpnI | GATC | 2 cut(s) | 9, 940 |
| DpnII | GATC | 2 cut(s) | 7, 938 |
| EaeI | YGGCCR | 2 cut(s) | 949, 997 |
| EciI | GGCGGA | 4 cut(s) | 38, 450, 924, 972 |
| Ecl136II | GAGCTC | 1 cut(s) | 872 |
| Eco130I | CCWWGG | 2 cut(s) | 887, 952 |
| Eco24I | GRGCYC | 2 cut(s) | 581, 874 |
| Eco31I | GGTCTC | 2 cut(s) | 174, 897 |
| Eco47I | GGWCC | 2 cut(s) | 152, 484 |
| Eco47III | AGCGCT | 1 cut(s) | 359 |
| Eco53kI | GAGCTC | 1 cut(s) | 872 |
| Eco88I | CYCGRG | 1 cut(s) | 228 |
| EcoICRI | GAGCTC | 1 cut(s) | 872 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 861 |
| EcoO109I | RGGNCCY | 1 cut(s) | 577 |
| EcoRII | CCWGG | 1 cut(s) | 856 |
| EcoT14I | CCWWGG | 2 cut(s) | 887, 952 |
| EcoT38I | GRGCYC | 2 cut(s) | 581, 874 |
| ErhI | CCWWGG | 2 cut(s) | 887, 952 |
| Esp3I | CGTCTC | 1 cut(s) | 1037 |
| FaeI | CATG | 3 cut(s) | 188, 689, 833 |
| FaiI | YATR | 6 cut(s) | 186, 676, 687, 722, 831, 970 |
| FaqI | GGGAC | 1 cut(s) | 1023 |
| FatI | CATG | 3 cut(s) | 184, 685, 829 |
| FauI | CCCGC | 7 cut(s) | 115, 588, 650, 694, 743, 809, 1000 |
| Fnu4HI | GCNGC | 9 cut(s) | 205, 270, 361, 372, 490, 679, 743, 852, 945 |
| FokI | GGATG | 2 cut(s) | 315, 488 |
| FriOI | GRGCYC | 2 cut(s) | 581, 874 |
| Fsp4HI | GCNGC | 9 cut(s) | 205, 270, 361, 372, 490, 679, 743, 852, 945 |
| FspBI | CTAG | 1 cut(s) | 747 |
| GlaI | GCGC | 4 cut(s) | 158, 359, 508, 762 |
| GluI | GCNGC | 9 cut(s) | 205, 270, 361, 372, 490, 679, 743, 852, 945 |
| GsuI | CTGGAG | 3 cut(s) | 198, 531, 879 |
| HaeII | RGCGCY | 2 cut(s) | 361, 510 |
| HaeIII | GGCC | 8 cut(s) | 207, 330, 406, 579, 597, 745, 951, 999 |
| HapII | CCGG | 6 cut(s) | 58, 111, 261, 312, 432, 493 |
| HgaI | GACGC | 3 cut(s) | 141, 832, 1019 |
| HhaI | GCGC | 4 cut(s) | 159, 360, 509, 763 |
| Hin1I | GRCGYC | 1 cut(s) | 843 |
| Hin1II | CATG | 3 cut(s) | 188, 689, 833 |
| Hin6I | GCGC | 4 cut(s) | 157, 358, 507, 761 |
| HinP1I | GCGC | 4 cut(s) | 157, 358, 507, 761 |
| HincII | GTYRAC | 1 cut(s) | 533 |
| HindII | GTYRAC | 1 cut(s) | 533 |
| HinfI | GANTC | 4 cut(s) | 125, 215, 534, 660 |
| HpaII | CCGG | 6 cut(s) | 58, 111, 261, 312, 432, 493 |
| HphI | GGTGA | 5 cut(s) | 233, 273, 309, 482, 708 |
| Hpy166II | GTNNAC | 4 cut(s) | 152, 190, 224, 533 |
| Hpy188I | TCNGA | 4 cut(s) | 12, 48, 55, 254 |
| Hpy188III | TCNNGA | 4 cut(s) | 129, 177, 686, 896 |
| Hpy8I | GTNNAC | 4 cut(s) | 152, 190, 224, 533 |
| Hpy99I | CGWCG | 4 cut(s) | 135, 470, 526, 614 |
| HpyAV | CCTTC | 4 cut(s) | 371, 413, 587, 911 |
| HpyCH4III | ACNGT | 3 cut(s) | 443, 620, 730 |
| HpyCH4IV | ACGT | 3 cut(s) | 521, 562, 994 |
| HpyCH4V | TGCA | 4 cut(s) | 320, 363, 428, 824 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 275, 317, 336, 380, 603, 675 |
| HpyF3I | CTNAG | 2 cut(s) | 243, 873 |
| HpySE526I | ACGT | 3 cut(s) | 521, 562, 994 |
| Hsp92I | GRCGYC | 1 cut(s) | 843 |
| Hsp92II | CATG | 3 cut(s) | 188, 689, 833 |
| HspAI | GCGC | 4 cut(s) | 157, 358, 507, 761 |
| KspI | CCGCGG | 1 cut(s) | 983 |
| Kzo9I | GATC | 2 cut(s) | 7, 938 |
| LmnI | GCTCC | 5 cut(s) | 380, 461, 611, 869, 1043 |
| Lsp1109I | GCAGC | 4 cut(s) | 256, 347, 383, 863 |
| LweI | GCATC | 1 cut(s) | 376 |
| MaeI | CTAG | 1 cut(s) | 747 |
| MaeII | ACGT | 3 cut(s) | 521, 562, 994 |
| MaeIII | GTNAC | 3 cut(s) | 67, 114, 963 |
| MalI | GATC | 2 cut(s) | 9, 940 |
| MbiI | CCGCTC | 2 cut(s) | 701, 1007 |
| MboI | GATC | 2 cut(s) | 7, 938 |
| MboII | GAAGA | 2 cut(s) | 224, 602 |
| MhlI | GDGCHC | 3 cut(s) | 581, 643, 874 |
| MlsI | TGGCCA | 2 cut(s) | 951, 999 |
| MluCI | AATT | 2 cut(s) | 32, 772 |
| MluI | ACGCGT | 1 cut(s) | 1028 |
| MluNI | TGGCCA | 2 cut(s) | 951, 999 |
| MlyI | GAGTC | 3 cut(s) | 134, 528, 654 |
| MmeI | TCCRAC | 2 cut(s) | 71, 1021 |
| Mox20I | TGGCCA | 2 cut(s) | 951, 999 |
| MscI | TGGCCA | 2 cut(s) | 951, 999 |
| MslI | CAYNNNNRTG | 2 cut(s) | 295, 834 |
| Msp20I | TGGCCA | 2 cut(s) | 951, 999 |
| MspA1I | CMGCKG | 1 cut(s) | 982 |
| MspI | CCGG | 6 cut(s) | 58, 111, 261, 312, 432, 493 |
| MspR9I | CCNGG | 3 cut(s) | 112, 494, 858 |
| MvaI | CCWGG | 1 cut(s) | 858 |
| MvnI | CGCG | 6 cut(s) | 130, 157, 159, 761, 982, 1030 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 275, 317, 336, 380, 603, 675 |
| NciI | CCSGG | 2 cut(s) | 112, 494 |
| NdeII | GATC | 2 cut(s) | 7, 938 |
| NlaIII | CATG | 3 cut(s) | 188, 689, 833 |
| NlaIV | GGNNCC | 7 cut(s) | 27, 259, 463, 498, 578, 579, 640 |
| NmeAIII | GCCGAG | 3 cut(s) | 569, 695, 774 |
| NmuCI | GTSAC | 1 cut(s) | 963 |
| NruI | TCGCGA | 1 cut(s) | 130 |
| NspI | RCATGY | 1 cut(s) | 833 |
| PagI | TCATGA | 1 cut(s) | 685 |
| PciI | ACATGT | 1 cut(s) | 829 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 832, 1000 |
| PfeI | GAWTC | 1 cut(s) | 215 |
| PinAI | ACCGGT | 1 cut(s) | 57 |
| PkrI | GCNGC | 9 cut(s) | 206, 271, 362, 373, 491, 680, 744, 853, 946 |
| PleI | GAGTC | 3 cut(s) | 133, 528, 654 |
| PpsI | GAGTC | 3 cut(s) | 133, 528, 654 |
| PscI | ACATGT | 1 cut(s) | 829 |
| Psp124BI | GAGCTC | 1 cut(s) | 874 |
| Psp6I | CCWGG | 1 cut(s) | 856 |
| PspGI | CCWGG | 1 cut(s) | 856 |
| PspN4I | GGNNCC | 7 cut(s) | 27, 259, 463, 498, 578, 579, 640 |
| PspOMI | GGGCCC | 1 cut(s) | 577 |
| PspPI | GGNCC | 8 cut(s) | 152, 206, 329, 405, 484, 577, 578, 596 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 666, 698 |
| PstI | CTGCAG | 1 cut(s) | 365 |
| PstNI | CAGNNNCTG | 1 cut(s) | 857 |
| RruI | TCGCGA | 1 cut(s) | 130 |
| RsaI | GTAC | 1 cut(s) | 189 |
| RsaNI | GTAC | 1 cut(s) | 188 |
| RseI | CAYNNNNRTG | 2 cut(s) | 295, 834 |
| SacI | GAGCTC | 1 cut(s) | 874 |
| SacII | CCGCGG | 1 cut(s) | 983 |
| SatI | GCNGC | 9 cut(s) | 205, 270, 361, 372, 490, 679, 743, 852, 945 |
| Sau3AI | GATC | 2 cut(s) | 7, 938 |
| Sau96I | GGNCC | 8 cut(s) | 152, 206, 329, 405, 484, 577, 578, 596 |
| SchI | GAGTC | 3 cut(s) | 134, 528, 654 |
| ScrFI | CCNGG | 3 cut(s) | 112, 494, 858 |
| SduI | GDGCHC | 3 cut(s) | 581, 643, 874 |
| SfaNI | GCATC | 1 cut(s) | 376 |
| SfcI | CTRYAG | 1 cut(s) | 361 |
| Sfr303I | CCGCGG | 1 cut(s) | 983 |
| SgrBI | CCGCGG | 1 cut(s) | 983 |
| SinI | GGWCC | 2 cut(s) | 152, 484 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 295, 834 |
| Sse9I | AATT | 2 cut(s) | 32, 772 |
| SspMI | CTAG | 1 cut(s) | 747 |
| SstI | GAGCTC | 1 cut(s) | 874 |
| StyD4I | CCNGG | 3 cut(s) | 110, 492, 856 |
| StyI | CCWWGG | 2 cut(s) | 887, 952 |
| TaaI | ACNGT | 3 cut(s) | 443, 620, 730 |
| TaiI | ACGT | 3 cut(s) | 524, 565, 997 |
| TaqI | TCGA | 5 cut(s) | 76, 123, 612, 862, 1003 |
| TasI | AATT | 2 cut(s) | 32, 772 |
| TatI | WGTACW | 1 cut(s) | 187 |
| TauI | GCSGC | 5 cut(s) | 207, 492, 681, 745, 947 |
| TfiI | GAWTC | 1 cut(s) | 215 |
| TscAI | CASTG | 3 cut(s) | 154, 241, 247 |
| TseFI | GTSAC | 1 cut(s) | 963 |
| TseI | GCWGC | 4 cut(s) | 269, 360, 371, 851 |
| Tsp45I | GTSAC | 1 cut(s) | 963 |
| TspDTI | ATGAA | 2 cut(s) | 674, 1039 |
| TspGWI | ACGGA | 2 cut(s) | 598, 783 |
| TspRI | CASTG | 3 cut(s) | 154, 241, 247 |
| VpaK11BI | GGWCC | 2 cut(s) | 152, 484 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 861 |
| XceI | RCATGY | 1 cut(s) | 833 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 291, 339 |
| XspI | CTAG | 1 cut(s) | 747 |