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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 2697, 2742 |
| AarI | CACCTGC | 2 cut(s) | 2025, 2080 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 2808, 3161 |
| AatII | GACGTC | 2 cut(s) | 644, 1800 |
| Acc16I | TGCGCA | 1 cut(s) | 2013 |
| Acc36I | ACCTGC | 5 cut(s) | 777, 822, 2025, 2080, 2258 |
| AccB1I | GGYRCC | 4 cut(s) | 1420, 1630, 2918, 2942 |
| AccBSI | CCGCTC | 2 cut(s) | 1693, 1720 |
| AccI | GTMKAC | 3 cut(s) | 704, 810, 2599 |
| AclWI | GGATC | 8 cut(s) | 749, 894, 1014, 1839, 2231, 2416, 2429, 3040 |
| AcoI | YGGCCR | 1 cut(s) | 1818 |
| AcsI | RAATTY | 3 cut(s) | 904, 1024, 2332 |
| AcuI | CTGAAG | 2 cut(s) | 690, 1765 |
| AcyI | GRCGYC | 5 cut(s) | 641, 1631, 1797, 2509, 2943 |
| AfaI | GTAC | 7 cut(s) | 625, 679, 1043, 1667, 2005, 2470, 2985 |
| AflII | CTTAAG | 1 cut(s) | 3106 |
| AflIII | ACRYGT | 4 cut(s) | 867, 1849, 2803, 3015 |
| AgsI | TTSAA | 8 cut(s) | 836, 1428, 2361, 2438, 2668, 2703, 3052, 3187 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2606 |
| AjiI | CACGTC | 1 cut(s) | 1852 |
| AleI | CACNNNNGTG | 2 cut(s) | 2142, 3176 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1682, 1714 |
| Alw21I | GWGCWC | 3 cut(s) | 660, 2149, 3207 |
| Alw44I | GTGCAC | 3 cut(s) | 656, 2145, 3203 |
| AlwI | GGATC | 8 cut(s) | 749, 894, 1014, 1839, 2231, 2416, 2429, 3040 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2108 |
| ApaLI | GTGCAC | 3 cut(s) | 656, 2145, 3203 |
| ApoI | RAATTY | 3 cut(s) | 904, 1024, 2332 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1213, 1360 |
| AsuC2I | CCSGG | 7 cut(s) | 633, 846, 990, 1082, 1222, 1369, 2312 |
| AsuHPI | GGTGA | 8 cut(s) | 643, 1136, 1319, 1466, 1483, 1985, 2089, 3161 |
| AsuNHI | GCTAGC | 3 cut(s) | 2111, 2182, 2191 |
| AvaII | GGWCC | 5 cut(s) | 1256, 1403, 1479, 1570, 2810 |
| BaeGI | GKGCMC | 3 cut(s) | 660, 2149, 3207 |
| BalI | TGGCCA | 1 cut(s) | 1820 |
| BamHI | GGATCC | 1 cut(s) | 2421 |
| BanI | GGYRCC | 4 cut(s) | 1420, 1630, 2918, 2942 |
| BauI | CACGAG | 2 cut(s) | 2293, 2729 |
| BbsI | GAAGAC | 1 cut(s) | 1421 |
| Bbv12I | GWGCWC | 3 cut(s) | 660, 2149, 3207 |
| BccI | CCATC | 9 cut(s) | 1117, 1151, 1187, 1334, 1646, 1716, 1829, 2412, 3140 |
| BceAI | ACGGC | 2 cut(s) | 1776, 2487 |
| BcnI | CCSGG | 7 cut(s) | 633, 846, 990, 1082, 1222, 1369, 2312 |
| BfaI | CTAG | 6 cut(s) | 2112, 2121, 2183, 2192, 2234, 2955 |
| BfmI | CTRYAG | 4 cut(s) | 1883, 2021, 2161, 2596 |
| BfoI | RGCGCY | 5 cut(s) | 1505, 1519, 1634, 2108, 2946 |
| BfrI | CTTAAG | 1 cut(s) | 3106 |
| BfuAI | ACCTGC | 5 cut(s) | 777, 822, 2025, 2080, 2258 |
| BlpI | GCTNAGC | 8 cut(s) | 77, 138, 219, 300, 381, 442, 485, 528 |
| Bme18I | GGWCC | 5 cut(s) | 1256, 1403, 1479, 1570, 2810 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2606 |
| BmgBI | CACGTC | 1 cut(s) | 1852 |
| BmiI | GGNNCC | 9 cut(s) | 761, 1113, 1422, 1571, 1632, 2227, 2423, 2920, 2944 |
| BmsI | GCATC | 7 cut(s) | 1227, 1374, 1610, 2103, 2175, 2951, 3202 |
| BmtI | GCTAGC | 3 cut(s) | 2115, 2186, 2195 |
| BoxI | GACNNNNGTC | 1 cut(s) | 3018 |
| BpiI | GAAGAC | 1 cut(s) | 1421 |
| BpmI | CTGGAG | 2 cut(s) | 1526, 1856 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2451 |
| Bpu1102I | GCTNAGC | 8 cut(s) | 77, 138, 219, 300, 381, 442, 485, 528 |
| BpuMI | CCSGG | 7 cut(s) | 633, 846, 990, 1082, 1222, 1369, 2312 |
| Bsa29I | ATCGAT | 1 cut(s) | 3025 |
| BsaBI | GATNNNNATC | 1 cut(s) | 3031 |
| BsaHI | GRCGYC | 5 cut(s) | 641, 1631, 1797, 2509, 2943 |
| BsaWI | WCCGGW | 1 cut(s) | 1114 |
| Bse118I | RCCGGY | 1 cut(s) | 1518 |
| Bse1I | ACTGG | 5 cut(s) | 796, 1138, 1316, 1953, 2368 |
| Bse3DI | GCAATG | 3 cut(s) | 1134, 3062, 3067 |
| Bse8I | GATNNNNATC | 1 cut(s) | 3031 |
| BseCI | ATCGAT | 1 cut(s) | 3025 |
| BseGI | GGATG | 8 cut(s) | 1198, 1242, 1345, 1389, 1821, 1957, 2679, 3217 |
| BseJI | GATNNNNATC | 1 cut(s) | 3031 |
| BseMI | GCAATG | 3 cut(s) | 1134, 3062, 3067 |
| BseNI | ACTGG | 5 cut(s) | 796, 1138, 1316, 1953, 2368 |
| BseRI | GAGGAG | 1 cut(s) | 2904 |
| BseSI | GKGCMC | 3 cut(s) | 660, 2149, 3207 |
| BseYI | CCCAGC | 6 cut(s) | 942, 946, 1062, 1723, 1966, 2178 |
| BsgI | GTGCAG | 4 cut(s) | 1193, 1295, 1946, 3066 |
| Bsh1285I | CGRYCG | 1 cut(s) | 664 |
| BshNI | GGYRCC | 4 cut(s) | 1420, 1630, 2918, 2942 |
| BshVI | ATCGAT | 1 cut(s) | 3025 |
| BsiEI | CGRYCG | 1 cut(s) | 664 |
| BsiHKAI | GWGCWC | 3 cut(s) | 660, 2149, 3207 |
| BsiWI | CGTACG | 1 cut(s) | 2468 |
| BslFI | GGGAC | 8 cut(s) | 37, 528, 708, 963, 1195, 1342, 2805, 3116 |
| BsmFI | GGGAC | 8 cut(s) | 37, 528, 708, 963, 1195, 1342, 2805, 3116 |
| Bsp1286I | GDGCHC | 3 cut(s) | 660, 2149, 3207 |
| Bsp143I | GATC | 9 cut(s) | 741, 886, 1006, 1645, 1831, 1872, 2236, 2421, 3032 |
| Bsp1720I | GCTNAGC | 8 cut(s) | 77, 138, 219, 300, 381, 442, 485, 528 |
| Bsp19I | CCATGG | 1 cut(s) | 882 |
| Bsp68I | TCGCGA | 1 cut(s) | 864 |
| BspDI | ATCGAT | 1 cut(s) | 3025 |
| BspHI | TCATGA | 2 cut(s) | 1677, 2685 |
| BspLI | GGNNCC | 9 cut(s) | 761, 1113, 1422, 1571, 1632, 2227, 2423, 2920, 2944 |
| BspMAI | CTGCAG | 2 cut(s) | 1887, 2025 |
| BspMI | ACCTGC | 5 cut(s) | 777, 822, 2025, 2080, 2258 |
| BspOI | GCTAGC | 3 cut(s) | 2115, 2186, 2195 |
| BspPI | GGATC | 8 cut(s) | 749, 894, 1014, 1839, 2231, 2416, 2429, 3040 |
| BspT107I | GGYRCC | 4 cut(s) | 1420, 1630, 2918, 2942 |
| BspTI | CTTAAG | 1 cut(s) | 3106 |
| BsrBI | CCGCTC | 2 cut(s) | 1693, 1720 |
| BsrDI | GCAATG | 3 cut(s) | 1134, 3062, 3067 |
| BsrFI | RCCGGY | 1 cut(s) | 1518 |
| BsrI | ACTGG | 5 cut(s) | 796, 1138, 1316, 1953, 2368 |
| BssAI | RCCGGY | 1 cut(s) | 1518 |
| BssMI | GATC | 9 cut(s) | 741, 886, 1006, 1645, 1831, 1872, 2236, 2421, 3032 |
| BssNAI | GTATAC | 1 cut(s) | 705 |
| BssNI | GRCGYC | 5 cut(s) | 641, 1631, 1797, 2509, 2943 |
| BssSI | CACGAG | 2 cut(s) | 2293, 2729 |
| BssT1I | CCWWGG | 4 cut(s) | 546, 882, 1147, 2323 |
| Bst1107I | GTATAC | 1 cut(s) | 705 |
| Bst2BI | CACGAG | 2 cut(s) | 2293, 2729 |
| Bst6I | CTCTTC | 3 cut(s) | 717, 1539, 1548 |
| BstACI | GRCGYC | 5 cut(s) | 641, 1631, 1797, 2509, 2943 |
| BstAFI | CTTAAG | 1 cut(s) | 3106 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 2020, 2108, 2259 |
| BstDSI | CCRYGG | 2 cut(s) | 882, 1801 |
| BstEII | GGTNACC | 1 cut(s) | 2837 |
| BstF5I | GGATG | 8 cut(s) | 1198, 1242, 1345, 1389, 1821, 1957, 2679, 3217 |
| BstH2I | RGCGCY | 5 cut(s) | 1505, 1519, 1634, 2108, 2946 |
| BstKTI | GATC | 9 cut(s) | 744, 889, 1009, 1648, 1834, 1875, 2239, 2424, 3035 |
| BstMBI | GATC | 9 cut(s) | 741, 886, 1006, 1645, 1831, 1872, 2236, 2421, 3032 |
| BstMCI | CGRYCG | 1 cut(s) | 664 |
| BstNSI | RCATGY | 3 cut(s) | 2711, 2807, 3019 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 3018 |
| BstPI | GGTNACC | 1 cut(s) | 2837 |
| BstSFI | CTRYAG | 4 cut(s) | 1883, 2021, 2161, 2596 |
| BstSLI | GKGCMC | 3 cut(s) | 660, 2149, 3207 |
| BstV2I | GAAGAC | 1 cut(s) | 1421 |
| BstX2I | RGATCY | 3 cut(s) | 1831, 2236, 2421 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2933 |
| BstYI | RGATCY | 3 cut(s) | 1831, 2236, 2421 |
| BstZ17I | GTATAC | 1 cut(s) | 705 |
| Bsu15I | ATCGAT | 1 cut(s) | 3025 |
| BsuTUI | ATCGAT | 1 cut(s) | 3025 |
| BtgI | CCRYGG | 2 cut(s) | 882, 1801 |
| BtrI | CACGTC | 1 cut(s) | 1852 |
| BtsCI | GGATG | 8 cut(s) | 1198, 1242, 1345, 1389, 1821, 1957, 2679, 3217 |
| BtsI | GCAGTG | 2 cut(s) | 1620, 2487 |
| BtsIMutI | CAGTG | 4 cut(s) | 1323, 1620, 1648, 2487 |
| BtuMI | TCGCGA | 1 cut(s) | 864 |
| BveI | ACCTGC | 5 cut(s) | 777, 822, 2025, 2080, 2258 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2108 |
| CciI | TCATGA | 2 cut(s) | 1677, 2685 |
| Cfr10I | RCCGGY | 1 cut(s) | 1518 |
| Cfr42I | CCGCGG | 1 cut(s) | 1804 |
| ClaI | ATCGAT | 1 cut(s) | 3025 |
| CseI | GACGC | 3 cut(s) | 721, 875, 2517 |
| Csp6I | GTAC | 7 cut(s) | 624, 678, 1042, 1666, 2004, 2469, 2984 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 670, 705 |
| CviQI | GTAC | 7 cut(s) | 624, 678, 1042, 1666, 2004, 2469, 2984 |
| DinI | GGCGCC | 2 cut(s) | 1632, 2944 |
| DpnI | GATC | 9 cut(s) | 743, 888, 1008, 1647, 1833, 1874, 2238, 2423, 3034 |
| DpnII | GATC | 9 cut(s) | 741, 886, 1006, 1645, 1831, 1872, 2236, 2421, 3032 |
| DraI | TTTAAA | 1 cut(s) | 2415 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 2808, 3161 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2606 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 2808, 3161 |
| EaeI | YGGCCR | 1 cut(s) | 1818 |
| Eam1104I | CTCTTC | 3 cut(s) | 717, 1539, 1548 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2606 |
| EarI | CTCTTC | 3 cut(s) | 717, 1539, 1548 |
| EciI | GGCGGA | 8 cut(s) | 772, 831, 908, 1028, 1205, 1352, 1562, 1634 |
| Eco130I | CCWWGG | 4 cut(s) | 546, 882, 1147, 2323 |
| Eco47I | GGWCC | 5 cut(s) | 1256, 1403, 1479, 1570, 2810 |
| Eco57I | CTGAAG | 2 cut(s) | 690, 1765 |
| Eco91I | GGTNACC | 1 cut(s) | 2837 |
| EcoO65I | GGTNACC | 1 cut(s) | 2837 |
| EcoT14I | CCWWGG | 4 cut(s) | 546, 882, 1147, 2323 |
| EgeI | GGCGCC | 2 cut(s) | 1632, 2944 |
| EheI | GGCGCC | 2 cut(s) | 1632, 2944 |
| ErhI | CCWWGG | 4 cut(s) | 546, 882, 1147, 2323 |
| FaqI | GGGAC | 8 cut(s) | 37, 528, 708, 963, 1195, 1342, 2805, 3116 |
| FblI | GTMKAC | 3 cut(s) | 704, 810, 2599 |
| FokI | GGATG | 8 cut(s) | 1205, 1249, 1352, 1396, 1808, 1964, 2666, 3224 |
| FspBI | CTAG | 6 cut(s) | 2112, 2121, 2183, 2192, 2234, 2955 |
| FspI | TGCGCA | 1 cut(s) | 2013 |
| GsaI | CCCAGC | 6 cut(s) | 946, 950, 1066, 1727, 1970, 2182 |
| GsuI | CTGGAG | 2 cut(s) | 1526, 1856 |
| HaeII | RGCGCY | 5 cut(s) | 1505, 1519, 1634, 2108, 2946 |
| HgaI | GACGC | 3 cut(s) | 721, 875, 2517 |
| Hin1I | GRCGYC | 5 cut(s) | 641, 1631, 1797, 2509, 2943 |
| HincII | GTYRAC | 2 cut(s) | 811, 3166 |
| HindII | GTYRAC | 2 cut(s) | 811, 3166 |
| HphI | GGTGA | 8 cut(s) | 643, 1136, 1319, 1466, 1483, 1985, 2089, 3161 |
| Hpy188I | TCNGA | 9 cut(s) | 746, 956, 1108, 2225, 2371, 2527, 2551, 2563, 2721 |
| Hpy99I | CGWCG | 4 cut(s) | 646, 869, 1762, 2511 |
| HpyCH4IV | ACGT | 5 cut(s) | 641, 1088, 1797, 1851, 2881 |
| HpySE526I | ACGT | 5 cut(s) | 641, 1088, 1797, 1851, 2881 |
| Hsp92I | GRCGYC | 5 cut(s) | 641, 1631, 1797, 2509, 2943 |
| KasI | GGCGCC | 2 cut(s) | 1630, 2942 |
| KroI | GCCGGC | 1 cut(s) | 1518 |
| KroNI | GCCGGC | 1 cut(s) | 1520 |
| KspI | CCGCGG | 1 cut(s) | 1804 |
| Kzo9I | GATC | 9 cut(s) | 741, 886, 1006, 1645, 1831, 1872, 2236, 2421, 3032 |
| LmnI | GCTCC | 5 cut(s) | 1675, 1690, 1888, 2225, 2770 |
| LweI | GCATC | 7 cut(s) | 1227, 1374, 1610, 2103, 2175, 2951, 3202 |
| MaeI | CTAG | 6 cut(s) | 2112, 2121, 2183, 2192, 2234, 2955 |
| MaeII | ACGT | 5 cut(s) | 641, 1088, 1797, 1851, 2881 |
| MaeIII | GTNAC | 9 cut(s) | 164, 245, 326, 649, 1100, 1471, 2386, 2608, 2837 |
| MalI | GATC | 9 cut(s) | 743, 888, 1008, 1647, 1833, 1874, 2238, 2423, 3034 |
| MbiI | CCGCTC | 2 cut(s) | 1693, 1720 |
| MboI | GATC | 9 cut(s) | 741, 886, 1006, 1645, 1831, 1872, 2236, 2421, 3032 |
| MfeI | CAATTG | 1 cut(s) | 1764 |
| MflI | RGATCY | 3 cut(s) | 1831, 2236, 2421 |
| MhlI | GDGCHC | 3 cut(s) | 660, 2149, 3207 |
| MlsI | TGGCCA | 1 cut(s) | 1820 |
| MluI | ACGCGT | 1 cut(s) | 867 |
| MluNI | TGGCCA | 1 cut(s) | 1820 |
| Mly113I | GGCGCC | 2 cut(s) | 1631, 2943 |
| MlyI | GAGTC | 9 cut(s) | 945, 1108, 1177, 1307, 1324, 1454, 1523, 1646, 1943 |
| MmeI | TCCRAC | 1 cut(s) | 2692 |
| Mox20I | TGGCCA | 1 cut(s) | 1820 |
| MroNI | GCCGGC | 1 cut(s) | 1518 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1213, 1360 |
| MscI | TGGCCA | 1 cut(s) | 1820 |
| MseI | TTAA | 5 cut(s) | 2414, 2501, 2538, 2993, 3107 |
| MslI | CAYNNNNRTG | 8 cut(s) | 1713, 1922, 2142, 2501, 2684, 3027, 3176, 3210 |
| Msp20I | TGGCCA | 1 cut(s) | 1820 |
| MspCI | CTTAAG | 1 cut(s) | 3106 |
| MunI | CAATTG | 1 cut(s) | 1764 |
| NaeI | GCCGGC | 1 cut(s) | 1520 |
| NarI | GGCGCC | 2 cut(s) | 1631, 2943 |
| NciI | CCSGG | 7 cut(s) | 633, 846, 990, 1082, 1222, 1369, 2312 |
| NcoI | CCATGG | 1 cut(s) | 882 |
| NdeII | GATC | 9 cut(s) | 741, 886, 1006, 1645, 1831, 1872, 2236, 2421, 3032 |
| NgoMIV | GCCGGC | 1 cut(s) | 1518 |
| NheI | GCTAGC | 3 cut(s) | 2111, 2182, 2191 |
| NlaIV | GGNNCC | 9 cut(s) | 761, 1113, 1422, 1571, 1632, 2227, 2423, 2920, 2944 |
| NmeAIII | GCCGAG | 5 cut(s) | 952, 1072, 1564, 1687, 1766 |
| NmuCI | GTSAC | 8 cut(s) | 164, 245, 326, 649, 1100, 1471, 2386, 2608 |
| NruI | TCGCGA | 1 cut(s) | 864 |
| NsbI | TGCGCA | 1 cut(s) | 2013 |
| NspI | RCATGY | 3 cut(s) | 2711, 2807, 3019 |
| OliI | CACNNNNGTG | 2 cut(s) | 2142, 3176 |
| PagI | TCATGA | 2 cut(s) | 1677, 2685 |
| PaqCI | CACCTGC | 2 cut(s) | 2025, 2080 |
| PciI | ACATGT | 2 cut(s) | 2803, 3015 |
| PdiI | GCCGGC | 1 cut(s) | 1520 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1213, 1360 |
| PfeI | GAWTC | 2 cut(s) | 2357, 2518 |
| Pfl23II | CGTACG | 1 cut(s) | 2468 |
| PflFI | GACNNNGTC | 9 cut(s) | 28, 89, 150, 231, 312, 393, 454, 497, 540 |
| PfoI | TCCNGGA | 5 cut(s) | 156, 237, 318, 399, 631 |
| PleI | GAGTC | 9 cut(s) | 945, 1107, 1177, 1306, 1324, 1453, 1523, 1646, 1942 |
| PluTI | GGCGCC | 2 cut(s) | 1634, 2946 |
| PpsI | GAGTC | 9 cut(s) | 945, 1107, 1177, 1306, 1324, 1453, 1523, 1646, 1942 |
| PscI | ACATGT | 2 cut(s) | 2803, 3015 |
| PshAI | GACNNNNGTC | 1 cut(s) | 3018 |
| PsiI | TTATAA | 2 cut(s) | 2697, 2742 |
| PspEI | GGTNACC | 1 cut(s) | 2837 |
| PspFI | CCCAGC | 6 cut(s) | 942, 946, 1062, 1723, 1966, 2178 |
| PspLI | CGTACG | 1 cut(s) | 2468 |
| PspN4I | GGNNCC | 9 cut(s) | 761, 1113, 1422, 1571, 1632, 2227, 2423, 2920, 2944 |
| PsrI | GAACNNNNNNTAC | 4 cut(s) | 1201, 1233, 1348, 1380 |
| PstI | CTGCAG | 2 cut(s) | 1887, 2025 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2108 |
| PsuI | RGATCY | 3 cut(s) | 1831, 2236, 2421 |
| PsyI | GACNNNGTC | 9 cut(s) | 28, 89, 150, 231, 312, 393, 454, 497, 540 |
| PvuII | CAGCTG | 2 cut(s) | 946, 2026 |
| RruI | TCGCGA | 1 cut(s) | 864 |
| RsaI | GTAC | 7 cut(s) | 625, 679, 1043, 1667, 2005, 2470, 2985 |
| RsaNI | GTAC | 7 cut(s) | 624, 678, 1042, 1666, 2004, 2469, 2984 |
| RseI | CAYNNNNRTG | 8 cut(s) | 1713, 1922, 2142, 2501, 2684, 3027, 3176, 3210 |
| SacII | CCGCGG | 1 cut(s) | 1804 |
| SalI | GTCGAC | 1 cut(s) | 809 |
| SaqAI | TTAA | 5 cut(s) | 2414, 2501, 2538, 2993, 3107 |
| Sau3AI | GATC | 9 cut(s) | 741, 886, 1006, 1645, 1831, 1872, 2236, 2421, 3032 |
| SchI | GAGTC | 9 cut(s) | 945, 1108, 1177, 1307, 1324, 1454, 1523, 1646, 1943 |
| SduI | GDGCHC | 3 cut(s) | 660, 2149, 3207 |
| SfaNI | GCATC | 7 cut(s) | 1227, 1374, 1610, 2103, 2175, 2951, 3202 |
| SfcI | CTRYAG | 4 cut(s) | 1883, 2021, 2161, 2596 |
| SfoI | GGCGCC | 2 cut(s) | 1632, 2944 |
| Sfr303I | CCGCGG | 1 cut(s) | 1804 |
| SgrBI | CCGCGG | 1 cut(s) | 1804 |
| SinI | GGWCC | 5 cut(s) | 1256, 1403, 1479, 1570, 2810 |
| SmiMI | CAYNNNNRTG | 8 cut(s) | 1713, 1922, 2142, 2501, 2684, 3027, 3176, 3210 |
| SmlI | CTYRAG | 1 cut(s) | 3106 |
| SmoI | CTYRAG | 1 cut(s) | 3106 |
| SspDI | GGCGCC | 2 cut(s) | 1630, 2942 |
| SspMI | CTAG | 6 cut(s) | 2112, 2121, 2183, 2192, 2234, 2955 |
| StyI | CCWWGG | 4 cut(s) | 546, 882, 1147, 2323 |
| TaiI | ACGT | 5 cut(s) | 644, 1091, 1800, 1854, 2884 |
| TaqI | TCGA | 6 cut(s) | 460, 503, 810, 1604, 2463, 3025 |
| TfiI | GAWTC | 2 cut(s) | 2357, 2518 |
| Tru1I | TTAA | 5 cut(s) | 2414, 2501, 2538, 2993, 3107 |
| Tru9I | TTAA | 5 cut(s) | 2414, 2501, 2538, 2993, 3107 |
| TscAI | CASTG | 4 cut(s) | 1323, 1620, 1648, 2494 |
| TseFI | GTSAC | 8 cut(s) | 164, 245, 326, 649, 1100, 1471, 2386, 2608 |
| Tsp45I | GTSAC | 8 cut(s) | 164, 245, 326, 649, 1100, 1471, 2386, 2608 |
| TspDTI | ATGAA | 5 cut(s) | 1666, 1860, 2531, 2702, 2865 |
| TspGWI | ACGGA | 5 cut(s) | 1249, 1298, 1396, 1445, 2814 |
| TspRI | CASTG | 4 cut(s) | 1323, 1620, 1648, 2494 |
| Tth111I | GACNNNGTC | 9 cut(s) | 28, 89, 150, 231, 312, 393, 454, 497, 540 |
| Vha464I | CTTAAG | 1 cut(s) | 3106 |
| VneI | GTGCAC | 3 cut(s) | 656, 2145, 3203 |
| VpaK11BI | GGWCC | 5 cut(s) | 1256, 1403, 1479, 1570, 2810 |
| XapI | RAATTY | 3 cut(s) | 904, 1024, 2332 |
| XceI | RCATGY | 3 cut(s) | 2711, 2807, 3019 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1145, 1645 |
| XmiI | GTMKAC | 3 cut(s) | 704, 810, 2599 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1213, 1360 |
| XspI | CTAG | 6 cut(s) | 2112, 2121, 2183, 2192, 2234, 2955 |
| ZraI | GACGTC | 2 cut(s) | 642, 1798 |