Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1240 |
| Acc36I | ACCTGC | 1 cut(s) | 1240 |
| Acc65I | GGTACC | 1 cut(s) | 299 |
| AccB1I | GGYRCC | 4 cut(s) | 299, 749, 1057, 1243 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 552 |
| AccBSI | CCGCTC | 3 cut(s) | 1336, 1396, 1549 |
| AccI | GTMKAC | 1 cut(s) | 903 |
| AccII | CGCG | 3 cut(s) | 125, 172, 690 |
| AccIII | TCCGGA | 1 cut(s) | 593 |
| AclWI | GGATC | 2 cut(s) | 496, 987 |
| AcoI | YGGCCR | 3 cut(s) | 691, 1077, 1378 |
| AcsI | RAATTY | 1 cut(s) | 515 |
| AcuI | CTGAAG | 1 cut(s) | 482 |
| AcyI | GRCGYC | 4 cut(s) | 151, 1058, 1170, 1244 |
| AfaI | GTAC | 3 cut(s) | 301, 437, 647 |
| AfeI | AGCGCT | 2 cut(s) | 226, 1475 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 195, 552, 585, 845, 875, 1086, 1387, 1536, 1594 |
| AflIII | ACRYGT | 1 cut(s) | 917 |
| AgsI | TTSAA | 1 cut(s) | 836 |
| AjiI | CACGTC | 1 cut(s) | 622 |
| AjnI | CCWGG | 8 cut(s) | 261, 281, 504, 693, 740, 1002, 1282, 1425 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1325, 1357 |
| AluBI | AGCT | 6 cut(s) | 358, 640, 709, 1402, 1435, 1519 |
| AluI | AGCT | 6 cut(s) | 358, 640, 709, 1402, 1435, 1519 |
| Alw21I | GWGCWC | 1 cut(s) | 1146 |
| Alw26I | GTCTC | 2 cut(s) | 1427, 1567 |
| AlwI | GGATC | 2 cut(s) | 496, 987 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 505 |
| Ama87I | CYCGRG | 3 cut(s) | 303, 1134, 1386 |
| Aor13HI | TCCGGA | 1 cut(s) | 593 |
| Aor51HI | AGCGCT | 2 cut(s) | 226, 1475 |
| ApaI | GGGCCC | 1 cut(s) | 1014 |
| ApoI | RAATTY | 1 cut(s) | 515 |
| Asp718I | GGTACC | 1 cut(s) | 299 |
| AspLEI | GCGC | 5 cut(s) | 172, 227, 1060, 1246, 1476 |
| AspS9I | GGNCC | 9 cut(s) | 286, 323, 605, 702, 803, 1010, 1011, 1037, 1113 |
| AsuC2I | CCSGG | 3 cut(s) | 1135, 1136, 1270 |
| AsuHPI | GGTGA | 2 cut(s) | 805, 1264 |
| AsuNHI | GCTAGC | 1 cut(s) | 211 |
| AvaI | CYCGRG | 3 cut(s) | 303, 1134, 1386 |
| AvaII | GGWCC | 3 cut(s) | 286, 605, 803 |
| BaeGI | GKGCMC | 1 cut(s) | 1014 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 291, 324 |
| BalI | TGGCCA | 2 cut(s) | 1079, 1380 |
| BanI | GGYRCC | 4 cut(s) | 299, 749, 1057, 1243 |
| BanII | GRGCYC | 1 cut(s) | 1014 |
| BauI | CACGAG | 2 cut(s) | 449, 1509 |
| Bbv12I | GWGCWC | 1 cut(s) | 1146 |
| BbvCI | CCTCAGC | 1 cut(s) | 1403 |
| BccI | CCATC | 4 cut(s) | 290, 1124, 1169, 1564 |
| BceAI | ACGGC | 1 cut(s) | 1514 |
| BciT130I | CCWGG | 8 cut(s) | 263, 283, 506, 695, 742, 1004, 1284, 1427 |
| BcnI | CCSGG | 3 cut(s) | 1135, 1136, 1270 |
| BcoDI | GTCTC | 2 cut(s) | 1427, 1567 |
| BfaI | CTAG | 6 cut(s) | 95, 165, 212, 375, 983, 1502 |
| BfmI | CTRYAG | 2 cut(s) | 110, 1436 |
| BfoI | RGCGCY | 4 cut(s) | 228, 1061, 1247, 1477 |
| BfuAI | ACCTGC | 1 cut(s) | 1240 |
| Bme18I | GGWCC | 3 cut(s) | 286, 605, 803 |
| BmeT110I | CYCGRG | 3 cut(s) | 303, 1134, 1386 |
| BmgBI | CACGTC | 1 cut(s) | 622 |
| BmgT120I | GGNCC | 9 cut(s) | 286, 323, 605, 702, 803, 1010, 1011, 1037, 1113 |
| BmsI | GCATC | 5 cut(s) | 425, 587, 981, 1486, 1495 |
| BmtI | GCTAGC | 1 cut(s) | 215 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1547, 1579 |
| BpmI | CTGGAG | 5 cut(s) | 284, 1025, 1056, 1112, 1409 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1403 |
| BpuEI | CTTGAG | 1 cut(s) | 221 |
| BpuMI | CCSGG | 3 cut(s) | 1135, 1136, 1270 |
| BsaAI | YACGTR | 1 cut(s) | 649 |
| BsaHI | GRCGYC | 4 cut(s) | 151, 1058, 1170, 1244 |
| BsaI | GGTCTC | 1 cut(s) | 1427 |
| BsaWI | WCCGGW | 1 cut(s) | 593 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1054, 1084, 1325, 1355 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 195, 552, 585, 845, 875, 1086, 1387, 1536, 1594 |
| Bse118I | RCCGGY | 1 cut(s) | 242 |
| Bse1I | ACTGG | 2 cut(s) | 951, 1073 |
| BseAI | TCCGGA | 1 cut(s) | 593 |
| BseBI | CCWGG | 8 cut(s) | 263, 283, 506, 695, 742, 1004, 1284, 1427 |
| BseGI | GGATG | 4 cut(s) | 416, 602, 1468, 1585 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 195, 552, 585, 845, 875, 1086, 1387, 1536, 1594 |
| BseMII | CTCAG | 1 cut(s) | 1394 |
| BseNI | ACTGG | 2 cut(s) | 951, 1073 |
| BseRI | GAGGAG | 4 cut(s) | 218, 266, 422, 1424 |
| BseSI | GKGCMC | 1 cut(s) | 1014 |
| BseYI | CCCAGC | 3 cut(s) | 146, 431, 937 |
| BsgI | GTGCAG | 3 cut(s) | 416, 611, 685 |
| Bsh1236I | CGCG | 3 cut(s) | 125, 172, 690 |
| Bsh1285I | CGRYCG | 1 cut(s) | 612 |
| BshNI | GGYRCC | 4 cut(s) | 299, 749, 1057, 1243 |
| BsiEI | CGRYCG | 1 cut(s) | 612 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1146 |
| BsiHKCI | CYCGRG | 3 cut(s) | 303, 1134, 1386 |
| BsiSI | CCGG | 4 cut(s) | 243, 594, 1135, 1269 |
| BslFI | GGGAC | 1 cut(s) | 1553 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 195, 552, 585, 845, 875, 1086, 1387, 1536, 1594 |
| BsmAI | GTCTC | 2 cut(s) | 1427, 1567 |
| BsmFI | GGGAC | 1 cut(s) | 1553 |
| BsmI | GAATGC | 1 cut(s) | 1447 |
| Bso31I | GGTCTC | 1 cut(s) | 1427 |
| BsoBI | CYCGRG | 3 cut(s) | 303, 1134, 1386 |
| Bsp120I | GGGCCC | 1 cut(s) | 1010 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1014, 1146 |
| Bsp13I | TCCGGA | 1 cut(s) | 593 |
| Bsp143I | GATC | 6 cut(s) | 501, 670, 711, 857, 979, 1413 |
| Bsp19I | CCATGG | 1 cut(s) | 975 |
| BspCNI | CTCAG | 1 cut(s) | 1395 |
| BspEI | TCCGGA | 1 cut(s) | 593 |
| BspFNI | CGCG | 3 cut(s) | 125, 172, 690 |
| BspMAI | CTGCAG | 1 cut(s) | 1440 |
| BspMI | ACCTGC | 1 cut(s) | 1240 |
| BspOI | GCTAGC | 1 cut(s) | 215 |
| BspPI | GGATC | 2 cut(s) | 496, 987 |
| BspT107I | GGYRCC | 4 cut(s) | 299, 749, 1057, 1243 |
| BspTNI | GGTCTC | 1 cut(s) | 1427 |
| BsrBI | CCGCTC | 3 cut(s) | 1336, 1396, 1549 |
| BsrFI | RCCGGY | 1 cut(s) | 242 |
| BsrI | ACTGG | 2 cut(s) | 951, 1073 |
| BssAI | RCCGGY | 1 cut(s) | 242 |
| BssMI | GATC | 6 cut(s) | 501, 670, 711, 857, 979, 1413 |
| BssNI | GRCGYC | 4 cut(s) | 151, 1058, 1170, 1244 |
| BssSI | CACGAG | 2 cut(s) | 449, 1509 |
| BssT1I | CCWWGG | 3 cut(s) | 466, 975, 1482 |
| Bst2BI | CACGAG | 2 cut(s) | 449, 1509 |
| Bst2UI | CCWGG | 8 cut(s) | 263, 283, 506, 695, 742, 1004, 1284, 1427 |
| Bst4CI | ACNGT | 4 cut(s) | 84, 334, 613, 1258 |
| Bst6I | CTCTTC | 1 cut(s) | 1352 |
| BstACI | GRCGYC | 4 cut(s) | 151, 1058, 1170, 1244 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1310 |
| BstBAI | YACGTR | 1 cut(s) | 649 |
| BstC8I | GCNNGC | 9 cut(s) | 213, 244, 259, 319, 323, 399, 429, 1216, 1517 |
| BstDEI | CTNAG | 2 cut(s) | 20, 1403 |
| BstDSI | CCRYGG | 4 cut(s) | 123, 612, 975, 1257 |
| BstEII | GGTNACC | 1 cut(s) | 1252 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1592 |
| BstF5I | GGATG | 4 cut(s) | 416, 602, 1468, 1585 |
| BstFNI | CGCG | 3 cut(s) | 125, 172, 690 |
| BstH2I | RGCGCY | 4 cut(s) | 228, 1061, 1247, 1477 |
| BstHHI | GCGC | 5 cut(s) | 172, 227, 1060, 1246, 1476 |
| BstKTI | GATC | 6 cut(s) | 504, 673, 714, 860, 982, 1416 |
| BstMAI | GTCTC | 2 cut(s) | 1427, 1567 |
| BstMBI | GATC | 6 cut(s) | 501, 670, 711, 857, 979, 1413 |
| BstMCI | CGRYCG | 1 cut(s) | 612 |
| BstNI | CCWGG | 8 cut(s) | 263, 283, 506, 695, 742, 1004, 1284, 1427 |
| BstPI | GGTNACC | 1 cut(s) | 1252 |
| BstSFI | CTRYAG | 2 cut(s) | 110, 1436 |
| BstSLI | GKGCMC | 1 cut(s) | 1014 |
| BstUI | CGCG | 3 cut(s) | 125, 172, 690 |
| BstX2I | RGATCY | 2 cut(s) | 501, 979 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 296 |
| BstYI | RGATCY | 2 cut(s) | 501, 979 |
| BtgI | CCRYGG | 4 cut(s) | 123, 612, 975, 1257 |
| BtgZI | GCGATG | 1 cut(s) | 1102 |
| BtrI | CACGTC | 1 cut(s) | 622 |
| BtsCI | GGATG | 4 cut(s) | 416, 602, 1468, 1585 |
| BtsI | GCAGTG | 1 cut(s) | 1080 |
| BtsIMutI | CAGTG | 2 cut(s) | 1080, 1080 |
| BveI | ACCTGC | 1 cut(s) | 1240 |
| Cac8I | GCNNGC | 9 cut(s) | 213, 244, 259, 319, 323, 399, 429, 1216, 1517 |
| CaiI | CAGNNNCTG | 1 cut(s) | 505 |
| CfoI | GCGC | 5 cut(s) | 172, 227, 1060, 1246, 1476 |
| Cfr10I | RCCGGY | 1 cut(s) | 242 |
| Cfr13I | GGNCC | 9 cut(s) | 286, 323, 605, 702, 803, 1010, 1011, 1037, 1113 |
| Cfr42I | CCGCGG | 1 cut(s) | 126 |
| Cfr9I | CCCGGG | 1 cut(s) | 1134 |
| CseI | GACGC | 6 cut(s) | 44, 140, 188, 820, 968, 1178 |
| Csp6I | GTAC | 3 cut(s) | 300, 436, 646 |
| CviAII | CATG | 2 cut(s) | 733, 976 |
| CviQI | GTAC | 3 cut(s) | 300, 436, 646 |
| DdeI | CTNAG | 2 cut(s) | 20, 1403 |
| DinI | GGCGCC | 2 cut(s) | 1059, 1245 |
| DpnI | GATC | 6 cut(s) | 503, 672, 713, 859, 981, 1415 |
| DpnII | GATC | 6 cut(s) | 501, 670, 711, 857, 979, 1413 |
| EaeI | YGGCCR | 3 cut(s) | 691, 1077, 1378 |
| Eam1104I | CTCTTC | 1 cut(s) | 1352 |
| EarI | CTCTTC | 1 cut(s) | 1352 |
| EciI | GGCGGA | 8 cut(s) | 44, 356, 452, 632, 727, 795, 894, 1475 |
| Eco130I | CCWWGG | 3 cut(s) | 466, 975, 1482 |
| Eco24I | GRGCYC | 1 cut(s) | 1014 |
| Eco31I | GGTCTC | 1 cut(s) | 1427 |
| Eco47I | GGWCC | 3 cut(s) | 286, 605, 803 |
| Eco47III | AGCGCT | 2 cut(s) | 226, 1475 |
| Eco57I | CTGAAG | 1 cut(s) | 482 |
| Eco88I | CYCGRG | 3 cut(s) | 303, 1134, 1386 |
| Eco91I | GGTNACC | 1 cut(s) | 1252 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1592 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1010, 1011 |
| EcoO65I | GGTNACC | 1 cut(s) | 1252 |
| EcoRI | GAATTC | 1 cut(s) | 515 |
| EcoRII | CCWGG | 8 cut(s) | 261, 281, 504, 693, 740, 1002, 1282, 1425 |
| EcoT14I | CCWWGG | 3 cut(s) | 466, 975, 1482 |
| EcoT38I | GRGCYC | 1 cut(s) | 1014 |
| EgeI | GGCGCC | 2 cut(s) | 1059, 1245 |
| EheI | GGCGCC | 2 cut(s) | 1059, 1245 |
| ErhI | CCWWGG | 3 cut(s) | 466, 975, 1482 |
| FaeI | CATG | 2 cut(s) | 736, 979 |
| FaqI | GGGAC | 1 cut(s) | 1553 |
| FatI | CATG | 2 cut(s) | 732, 975 |
| FauI | CCCGC | 7 cut(s) | 130, 239, 314, 384, 763, 1030, 1329 |
| FauNDI | CATATG | 1 cut(s) | 551 |
| FblI | GTMKAC | 1 cut(s) | 903 |
| FokI | GGATG | 4 cut(s) | 403, 609, 1475, 1592 |
| FriOI | GRGCYC | 1 cut(s) | 1014 |
| FspBI | CTAG | 6 cut(s) | 95, 165, 212, 375, 983, 1502 |
| GlaI | GCGC | 5 cut(s) | 171, 226, 1059, 1245, 1475 |
| GsaI | CCCAGC | 3 cut(s) | 150, 435, 941 |
| GsuI | CTGGAG | 5 cut(s) | 284, 1025, 1056, 1112, 1409 |
| HaeII | RGCGCY | 4 cut(s) | 228, 1061, 1247, 1477 |
| HapII | CCGG | 4 cut(s) | 243, 594, 1135, 1269 |
| HgaI | GACGC | 6 cut(s) | 44, 140, 188, 820, 968, 1178 |
| HhaI | GCGC | 5 cut(s) | 172, 227, 1060, 1246, 1476 |
| Hin1I | GRCGYC | 4 cut(s) | 151, 1058, 1170, 1244 |
| Hin1II | CATG | 2 cut(s) | 736, 979 |
| Hin6I | GCGC | 5 cut(s) | 170, 225, 1058, 1244, 1474 |
| HinP1I | GCGC | 5 cut(s) | 170, 225, 1058, 1244, 1474 |
| HincII | GTYRAC | 1 cut(s) | 904 |
| HindII | GTYRAC | 1 cut(s) | 904 |
| HinfI | GANTC | 6 cut(s) | 47, 275, 728, 929, 1295, 1328 |
| HpaII | CCGG | 4 cut(s) | 243, 594, 1135, 1269 |
| HphI | GGTGA | 2 cut(s) | 805, 1264 |
| Hpy166II | GTNNAC | 5 cut(s) | 310, 346, 475, 646, 904 |
| Hpy188I | TCNGA | 7 cut(s) | 10, 501, 521, 609, 829, 845, 1333 |
| Hpy188III | TCNNGA | 5 cut(s) | 155, 594, 715, 1292, 1321 |
| Hpy8I | GTNNAC | 5 cut(s) | 310, 346, 475, 646, 904 |
| Hpy99I | CGWCG | 5 cut(s) | 814, 908, 962, 1035, 1211 |
| HpyAV | CCTTC | 7 cut(s) | 380, 506, 718, 1045, 1060, 1222, 1588 |
| HpyCH4III | ACNGT | 4 cut(s) | 84, 334, 613, 1258 |
| HpyCH4IV | ACGT | 7 cut(s) | 12, 129, 621, 648, 906, 919, 1542 |
| HpyCH4V | TGCA | 9 cut(s) | 71, 397, 628, 666, 764, 994, 1085, 1304, 1438 |
| HpyF3I | CTNAG | 2 cut(s) | 20, 1403 |
| HpySE526I | ACGT | 7 cut(s) | 12, 129, 621, 648, 906, 919, 1542 |
| Hsp92I | GRCGYC | 4 cut(s) | 151, 1058, 1170, 1244 |
| Hsp92II | CATG | 2 cut(s) | 736, 979 |
| HspAI | GCGC | 5 cut(s) | 170, 225, 1058, 1244, 1474 |
| KasI | GGCGCC | 2 cut(s) | 1057, 1243 |
| Kpn2I | TCCGGA | 1 cut(s) | 593 |
| KpnI | GGTACC | 1 cut(s) | 303 |
| KroI | GCCGGC | 1 cut(s) | 242 |
| KroNI | GCCGGC | 1 cut(s) | 244 |
| KspI | CCGCGG | 1 cut(s) | 126 |
| Kzo9I | GATC | 6 cut(s) | 501, 670, 711, 857, 979, 1413 |
| LmnI | GCTCC | 5 cut(s) | 1075, 1093, 1149, 1333, 1432 |
| LweI | GCATC | 5 cut(s) | 425, 587, 981, 1486, 1495 |
| MaeI | CTAG | 6 cut(s) | 95, 165, 212, 375, 983, 1502 |
| MaeII | ACGT | 7 cut(s) | 12, 129, 621, 648, 906, 919, 1542 |
| MaeIII | GTNAC | 1 cut(s) | 1252 |
| MalI | GATC | 6 cut(s) | 503, 672, 713, 859, 981, 1415 |
| MbiI | CCGCTC | 3 cut(s) | 1336, 1396, 1549 |
| MboI | GATC | 6 cut(s) | 501, 670, 711, 857, 979, 1413 |
| MboII | GAAGA | 7 cut(s) | 451, 1000, 1193, 1196, 1199, 1202, 1339 |
| MflI | RGATCY | 2 cut(s) | 501, 979 |
| MhlI | GDGCHC | 2 cut(s) | 1014, 1146 |
| MlsI | TGGCCA | 2 cut(s) | 1079, 1380 |
| MluCI | AATT | 3 cut(s) | 351, 515, 574 |
| MluNI | TGGCCA | 2 cut(s) | 1079, 1380 |
| Mly113I | GGCGCC | 2 cut(s) | 1058, 1244 |
| MlyI | GAGTC | 2 cut(s) | 284, 1322 |
| MmeI | TCCRAC | 2 cut(s) | 632, 807 |
| Mox20I | TGGCCA | 2 cut(s) | 1079, 1380 |
| MroI | TCCGGA | 1 cut(s) | 593 |
| MroNI | GCCGGC | 1 cut(s) | 242 |
| MscI | TGGCCA | 2 cut(s) | 1079, 1380 |
| MseI | TTAA | 5 cut(s) | 354, 573, 678, 822, 1600 |
| MslI | CAYNNNNRTG | 1 cut(s) | 294 |
| Msp20I | TGGCCA | 2 cut(s) | 1079, 1380 |
| MspA1I | CMGCKG | 7 cut(s) | 125, 321, 484, 770, 865, 937, 1402 |
| MspI | CCGG | 4 cut(s) | 243, 594, 1135, 1269 |
| Mva1269I | GAATGC | 1 cut(s) | 1447 |
| MvaI | CCWGG | 8 cut(s) | 263, 283, 506, 695, 742, 1004, 1284, 1427 |
| MvnI | CGCG | 3 cut(s) | 125, 172, 690 |
| NaeI | GCCGGC | 1 cut(s) | 244 |
| NarI | GGCGCC | 2 cut(s) | 1058, 1244 |
| NciI | CCSGG | 3 cut(s) | 1135, 1136, 1270 |
| NcoI | CCATGG | 1 cut(s) | 975 |
| NdeI | CATATG | 1 cut(s) | 551 |
| NdeII | GATC | 6 cut(s) | 501, 670, 711, 857, 979, 1413 |
| NgoMIV | GCCGGC | 1 cut(s) | 242 |
| NheI | GCTAGC | 1 cut(s) | 211 |
| NlaIII | CATG | 2 cut(s) | 736, 979 |
| NmeAIII | GCCGAG | 1 cut(s) | 1339 |
| NmuCI | GTSAC | 1 cut(s) | 1252 |
| PaqCI | CACCTGC | 1 cut(s) | 1240 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 148 |
| PctI | GAATGC | 1 cut(s) | 1447 |
| PdiI | GCCGGC | 1 cut(s) | 244 |
| PfeI | GAWTC | 4 cut(s) | 47, 728, 929, 1295 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 552 |
| PfoI | TCCNGGA | 1 cut(s) | 1002 |
| PleI | GAGTC | 2 cut(s) | 283, 1322 |
| PluTI | GGCGCC | 2 cut(s) | 1061, 1247 |
| PpsI | GAGTC | 2 cut(s) | 283, 1322 |
| Ppu21I | YACGTR | 1 cut(s) | 649 |
| Psp6I | CCWGG | 8 cut(s) | 261, 281, 504, 693, 740, 1002, 1282, 1425 |
| PspEI | GGTNACC | 1 cut(s) | 1252 |
| PspFI | CCCAGC | 3 cut(s) | 146, 431, 937 |
| PspGI | CCWGG | 8 cut(s) | 261, 281, 504, 693, 740, 1002, 1282, 1425 |
| PspOMI | GGGCCC | 1 cut(s) | 1010 |
| PspPI | GGNCC | 9 cut(s) | 286, 323, 605, 702, 803, 1010, 1011, 1037, 1113 |
| PstI | CTGCAG | 1 cut(s) | 1440 |
| PstNI | CAGNNNCTG | 1 cut(s) | 505 |
| PsuI | RGATCY | 2 cut(s) | 501, 979 |
| PvuII | CAGCTG | 1 cut(s) | 1402 |
| RsaI | GTAC | 3 cut(s) | 301, 437, 647 |
| RsaNI | GTAC | 3 cut(s) | 300, 436, 646 |
| RseI | CAYNNNNRTG | 1 cut(s) | 294 |
| SacII | CCGCGG | 1 cut(s) | 126 |
| SalI | GTCGAC | 1 cut(s) | 902 |
| SaqAI | TTAA | 5 cut(s) | 354, 573, 678, 822, 1600 |
| Sau3AI | GATC | 6 cut(s) | 501, 670, 711, 857, 979, 1413 |
| Sau96I | GGNCC | 9 cut(s) | 286, 323, 605, 702, 803, 1010, 1011, 1037, 1113 |
| SchI | GAGTC | 2 cut(s) | 284, 1322 |
| SduI | GDGCHC | 2 cut(s) | 1014, 1146 |
| SfaNI | GCATC | 5 cut(s) | 425, 587, 981, 1486, 1495 |
| SfcI | CTRYAG | 2 cut(s) | 110, 1436 |
| SfoI | GGCGCC | 2 cut(s) | 1059, 1245 |
| Sfr303I | CCGCGG | 1 cut(s) | 126 |
| SgrBI | CCGCGG | 1 cut(s) | 126 |
| SinI | GGWCC | 3 cut(s) | 286, 605, 803 |
| SmaI | CCCGGG | 1 cut(s) | 1136 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 294 |
| SmlI | CTYRAG | 1 cut(s) | 200 |
| SmoI | CTYRAG | 1 cut(s) | 200 |
| Sse9I | AATT | 3 cut(s) | 351, 515, 574 |
| SspDI | GGCGCC | 2 cut(s) | 1057, 1243 |
| SspMI | CTAG | 6 cut(s) | 95, 165, 212, 375, 983, 1502 |
| StyI | CCWWGG | 3 cut(s) | 466, 975, 1482 |
| TaaI | ACNGT | 4 cut(s) | 84, 334, 613, 1258 |
| TaiI | ACGT | 7 cut(s) | 15, 132, 624, 651, 909, 922, 1545 |
| TaqI | TCGA | 5 cut(s) | 102, 273, 714, 903, 1209 |
| TasI | AATT | 3 cut(s) | 351, 515, 574 |
| TfiI | GAWTC | 4 cut(s) | 47, 728, 929, 1295 |
| Tru1I | TTAA | 5 cut(s) | 354, 573, 678, 822, 1600 |
| Tru9I | TTAA | 5 cut(s) | 354, 573, 678, 822, 1600 |
| TscAI | CASTG | 2 cut(s) | 1080, 1087 |
| TseFI | GTSAC | 1 cut(s) | 1252 |
| Tsp45I | GTSAC | 1 cut(s) | 1252 |
| TspDTI | ATGAA | 2 cut(s) | 737, 1287 |
| TspGWI | ACGGA | 2 cut(s) | 48, 913 |
| TspMI | CCCGGG | 1 cut(s) | 1134 |
| TspRI | CASTG | 2 cut(s) | 1080, 1087 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 552 |
| VpaK11BI | GGWCC | 3 cut(s) | 286, 605, 803 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1592 |
| XapI | RAATTY | 1 cut(s) | 515 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1087, 1453 |
| XmaI | CCCGGG | 1 cut(s) | 1134 |
| XmiI | GTMKAC | 1 cut(s) | 903 |
| XspI | CTAG | 6 cut(s) | 95, 165, 212, 375, 983, 1502 |