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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 865 |
| Acc36I | ACCTGC | 2 cut(s) | 750, 1975 |
| Acc65I | GGTACC | 1 cut(s) | 1214 |
| AccB1I | GGYRCC | 5 cut(s) | 652, 1144, 1214, 1581, 2093 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 159, 457, 1947 |
| AccBSI | CCGCTC | 6 cut(s) | 834, 1371, 1420, 1543, 1579, 1645 |
| AccI | GTMKAC | 1 cut(s) | 597 |
| AccII | CGCG | 3 cut(s) | 246, 801, 1620 |
| AccIII | TCCGGA | 2 cut(s) | 190, 2005 |
| AclWI | GGATC | 5 cut(s) | 551, 564, 926, 1713, 2128 |
| AcoI | YGGCCR | 3 cut(s) | 184, 1635, 2070 |
| AcsI | RAATTY | 5 cut(s) | 453, 670, 1400, 1424, 1496 |
| AcuI | CTGAAG | 6 cut(s) | 374, 1217, 1352, 1532, 1721, 1727 |
| AcyI | GRCGYC | 1 cut(s) | 1582 |
| AfaI | GTAC | 3 cut(s) | 253, 896, 1216 |
| AflIII | ACRYGT | 2 cut(s) | 48, 1920 |
| AgeI | ACCGGT | 1 cut(s) | 764 |
| AgsI | TTSAA | 5 cut(s) | 307, 676, 1927, 2042, 2168 |
| AjiI | CACGTC | 1 cut(s) | 49 |
| AjnI | CCWGG | 6 cut(s) | 325, 417, 645, 906, 1970, 2116 |
| AluBI | AGCT | 7 cut(s) | 340, 775, 1564, 1627, 1694, 1898, 2141 |
| AluI | AGCT | 7 cut(s) | 340, 775, 1564, 1627, 1694, 1898, 2141 |
| Alw21I | GWGCWC | 1 cut(s) | 402 |
| Alw26I | GTCTC | 4 cut(s) | 693, 1280, 1535, 1544 |
| Alw44I | GTGCAC | 1 cut(s) | 398 |
| AlwI | GGATC | 5 cut(s) | 551, 564, 926, 1713, 2128 |
| Ama87I | CYCGRG | 4 cut(s) | 22, 1652, 2082, 2186 |
| Aor13HI | TCCGGA | 2 cut(s) | 190, 2005 |
| ApaI | GGGCCC | 2 cut(s) | 1128, 1300 |
| ApaLI | GTGCAC | 1 cut(s) | 398 |
| ApeKI | GCWGC | 8 cut(s) | 322, 340, 577, 973, 976, 1079, 1904, 2180 |
| ApoI | RAATTY | 5 cut(s) | 453, 670, 1400, 1424, 1496 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1041, 1073 |
| AseI | ATTAAT | 1 cut(s) | 873 |
| AsiGI | ACCGGT | 1 cut(s) | 764 |
| Asp718I | GGTACC | 1 cut(s) | 1214 |
| AspLEI | GCGC | 5 cut(s) | 523, 803, 866, 1084, 1584 |
| AsuC2I | CCSGG | 3 cut(s) | 40, 819, 1801 |
| AsuHPI | GGTGA | 7 cut(s) | 640, 754, 1348, 1802, 1903, 1963, 2009 |
| AsuNHI | GCTAGC | 2 cut(s) | 5, 902 |
| AvaI | CYCGRG | 4 cut(s) | 22, 1652, 2082, 2186 |
| AvaII | GGWCC | 4 cut(s) | 275, 701, 1072, 1648 |
| BaeGI | GKGCMC | 5 cut(s) | 402, 657, 1128, 1149, 1300 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 243, 276 |
| BalI | TGGCCA | 1 cut(s) | 1637 |
| BamHI | GGATCC | 1 cut(s) | 556 |
| BanI | GGYRCC | 5 cut(s) | 652, 1144, 1214, 1581, 2093 |
| BanII | GRGCYC | 4 cut(s) | 1128, 1300, 1462, 2108 |
| BauI | CACGAG | 2 cut(s) | 401, 717 |
| BbsI | GAAGAC | 1 cut(s) | 749 |
| Bbv12I | GWGCWC | 1 cut(s) | 402 |
| BbvI | GCAGC | 8 cut(s) | 309, 327, 564, 960, 963, 1066, 1891, 2167 |
| BccI | CCATC | 6 cut(s) | 242, 511, 919, 1876, 1949, 2084 |
| BceAI | ACGGC | 5 cut(s) | 171, 278, 380, 1211, 2057 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 827, 861 |
| BciT130I | CCWGG | 6 cut(s) | 327, 419, 647, 908, 1972, 2118 |
| BciVI | GTATCC | 1 cut(s) | 208 |
| BclI | TGATCA | 1 cut(s) | 2125 |
| BcnI | CCSGG | 3 cut(s) | 40, 819, 1801 |
| BcoDI | GTCTC | 4 cut(s) | 693, 1280, 1535, 1544 |
| BfaI | CTAG | 6 cut(s) | 6, 131, 201, 903, 1679, 2138 |
| BfmI | CTRYAG | 3 cut(s) | 146, 1839, 1860 |
| BfoI | RGCGCY | 2 cut(s) | 524, 1585 |
| BfuAI | ACCTGC | 2 cut(s) | 750, 1975 |
| BfuI | GTATCC | 1 cut(s) | 208 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1052 |
| BglII | AGATCT | 1 cut(s) | 18 |
| BlpI | GCTNAGC | 1 cut(s) | 915 |
| Bme1390I | CCNGG | 9 cut(s) | 40, 327, 419, 647, 819, 908, 1801, 1972, 2118 |
| Bme18I | GGWCC | 4 cut(s) | 275, 701, 1072, 1648 |
| BmeT110I | CYCGRG | 4 cut(s) | 22, 1652, 2082, 2186 |
| BmgBI | CACGTC | 1 cut(s) | 49 |
| BmrFI | CCNGG | 9 cut(s) | 40, 327, 419, 647, 819, 908, 1801, 1972, 2118 |
| BmrI | ACTGGG | 2 cut(s) | 962, 1957 |
| BmsI | GCATC | 5 cut(s) | 363, 829, 972, 1179, 1603 |
| BmtI | GCTAGC | 2 cut(s) | 9, 906 |
| BmuI | ACTGGG | 2 cut(s) | 962, 1957 |
| BoxI | GACNNNNGTC | 2 cut(s) | 753, 926 |
| BpiI | GAAGAC | 1 cut(s) | 749 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1589, 1621 |
| BpmI | CTGGAG | 6 cut(s) | 291, 905, 1199, 1517, 1826, 2180 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1416 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 915 |
| BpuEI | CTTGAG | 2 cut(s) | 655, 1446 |
| BpuMI | CCSGG | 3 cut(s) | 40, 819, 1801 |
| BsaAI | YACGTR | 1 cut(s) | 601 |
| BsaHI | GRCGYC | 1 cut(s) | 1582 |
| BsaI | GGTCTC | 1 cut(s) | 1535 |
| BsaWI | WCCGGW | 4 cut(s) | 190, 545, 764, 2005 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 878, 908, 1655, 1685, 2017, 2047 |
| Bse118I | RCCGGY | 2 cut(s) | 182, 764 |
| Bse1I | ACTGG | 3 cut(s) | 968, 1160, 1952 |
| Bse3DI | GCAATG | 1 cut(s) | 177 |
| BseAI | TCCGGA | 2 cut(s) | 190, 2005 |
| BseBI | CCWGG | 6 cut(s) | 327, 419, 647, 908, 1972, 2118 |
| BseGI | GGATG | 2 cut(s) | 364, 1618 |
| BseMI | GCAATG | 1 cut(s) | 177 |
| BseMII | CTCAG | 7 cut(s) | 929, 1235, 1407, 1465, 1803, 1818, 2033 |
| BseNI | ACTGG | 3 cut(s) | 968, 1160, 1952 |
| BseSI | GKGCMC | 5 cut(s) | 402, 657, 1128, 1149, 1300 |
| BseX3I | CGGCCG | 1 cut(s) | 184 |
| BseXI | GCAGC | 8 cut(s) | 309, 327, 564, 960, 963, 1066, 1891, 2167 |
| BseYI | CCCAGC | 2 cut(s) | 1116, 2141 |
| BsgI | GTGCAG | 1 cut(s) | 563 |
| Bsh1236I | CGCG | 3 cut(s) | 246, 801, 1620 |
| Bsh1285I | CGRYCG | 1 cut(s) | 187 |
| BshNI | GGYRCC | 5 cut(s) | 652, 1144, 1214, 1581, 2093 |
| BshTI | ACCGGT | 1 cut(s) | 764 |
| BsiEI | CGRYCG | 1 cut(s) | 187 |
| BsiHKAI | GWGCWC | 1 cut(s) | 402 |
| BsiHKCI | CYCGRG | 4 cut(s) | 22, 1652, 2082, 2186 |
| BsiSI | CCGG | 9 cut(s) | 39, 183, 191, 319, 546, 765, 818, 1801, 2006 |
| BslFI | GGGAC | 4 cut(s) | 714, 1058, 1661, 1945 |
| BsmAI | GTCTC | 4 cut(s) | 693, 1280, 1535, 1544 |
| BsmFI | GGGAC | 4 cut(s) | 714, 1058, 1661, 1945 |
| Bso31I | GGTCTC | 1 cut(s) | 1535 |
| BsoBI | CYCGRG | 4 cut(s) | 22, 1652, 2082, 2186 |
| Bsp120I | GGGCCC | 2 cut(s) | 1124, 1296 |
| Bsp1286I | GDGCHC | 7 cut(s) | 402, 657, 1128, 1149, 1300, 1462, 2108 |
| Bsp13I | TCCGGA | 2 cut(s) | 190, 2005 |
| Bsp143I | GATC | 8 cut(s) | 18, 408, 556, 784, 931, 1705, 2120, 2125 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 915 |
| Bsp19I | CCATGG | 1 cut(s) | 1608 |
| BspCNI | CTCAG | 7 cut(s) | 928, 1234, 1408, 1464, 1804, 1819, 2032 |
| BspEI | TCCGGA | 2 cut(s) | 190, 2005 |
| BspFNI | CGCG | 3 cut(s) | 246, 801, 1620 |
| BspMI | ACCTGC | 2 cut(s) | 750, 1975 |
| BspOI | GCTAGC | 2 cut(s) | 9, 906 |
| BspPI | GGATC | 5 cut(s) | 551, 564, 926, 1713, 2128 |
| BspQI | GCTCTTC | 1 cut(s) | 1811 |
| BspT107I | GGYRCC | 5 cut(s) | 652, 1144, 1214, 1581, 2093 |
| BspTNI | GGTCTC | 1 cut(s) | 1535 |
| BsrBI | CCGCTC | 6 cut(s) | 834, 1371, 1420, 1543, 1579, 1645 |
| BsrDI | GCAATG | 1 cut(s) | 177 |
| BsrFI | RCCGGY | 2 cut(s) | 182, 764 |
| BsrI | ACTGG | 3 cut(s) | 968, 1160, 1952 |
| BssAI | RCCGGY | 2 cut(s) | 182, 764 |
| BssMI | GATC | 8 cut(s) | 18, 408, 556, 784, 931, 1705, 2120, 2125 |
| BssNI | GRCGYC | 1 cut(s) | 1582 |
| BssSI | CACGAG | 2 cut(s) | 401, 717 |
| BssT1I | CCWWGG | 7 cut(s) | 158, 610, 692, 1094, 1152, 1590, 1608 |
| Bst2BI | CACGAG | 2 cut(s) | 401, 717 |
| Bst2UI | CCWGG | 6 cut(s) | 327, 419, 647, 908, 1972, 2118 |
| Bst4CI | ACNGT | 6 cut(s) | 120, 286, 565, 792, 1013, 1840 |
| Bst6I | CTCTTC | 5 cut(s) | 1263, 1304, 1436, 1811, 2163 |
| BstACI | GRCGYC | 1 cut(s) | 1582 |
| BstBAI | YACGTR | 1 cut(s) | 601 |
| BstDEI | CTNAG | 7 cut(s) | 915, 1221, 1416, 1451, 1812, 1827, 2019 |
| BstDSI | CCRYGG | 3 cut(s) | 564, 1608, 1618 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1589, 1826 |
| BstF5I | GGATG | 2 cut(s) | 364, 1618 |
| BstFNI | CGCG | 3 cut(s) | 246, 801, 1620 |
| BstH2I | RGCGCY | 2 cut(s) | 524, 1585 |
| BstHHI | GCGC | 5 cut(s) | 523, 803, 866, 1084, 1584 |
| BstKTI | GATC | 8 cut(s) | 21, 411, 559, 787, 934, 1708, 2123, 2128 |
| BstMAI | GTCTC | 4 cut(s) | 693, 1280, 1535, 1544 |
| BstMBI | GATC | 8 cut(s) | 18, 408, 556, 784, 931, 1705, 2120, 2125 |
| BstMCI | CGRYCG | 1 cut(s) | 187 |
| BstNI | CCWGG | 6 cut(s) | 327, 419, 647, 908, 1972, 2118 |
| BstNSI | RCATGY | 3 cut(s) | 392, 895, 1924 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 753, 926 |
| BstSCI | CCNGG | 9 cut(s) | 38, 325, 417, 645, 817, 906, 1799, 1970, 2116 |
| BstSFI | CTRYAG | 3 cut(s) | 146, 1839, 1860 |
| BstSLI | GKGCMC | 5 cut(s) | 402, 657, 1128, 1149, 1300 |
| BstUI | CGCG | 3 cut(s) | 246, 801, 1620 |
| BstV1I | GCAGC | 8 cut(s) | 309, 327, 564, 960, 963, 1066, 1891, 2167 |
| BstV2I | GAAGAC | 1 cut(s) | 749 |
| BstX2I | RGATCY | 3 cut(s) | 18, 556, 2120 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 617, 1768 |
| BstYI | RGATCY | 3 cut(s) | 18, 556, 2120 |
| BstZI | CGGCCG | 1 cut(s) | 184 |
| BsuI | GTATCC | 1 cut(s) | 208 |
| BtgI | CCRYGG | 3 cut(s) | 564, 1608, 1618 |
| BtgZI | GCGATG | 2 cut(s) | 261, 852 |
| BtrI | CACGTC | 1 cut(s) | 49 |
| BtsCI | GGATG | 2 cut(s) | 364, 1618 |
| BtsI | GCAGTG | 1 cut(s) | 34 |
| BtsIMutI | CAGTG | 7 cut(s) | 34, 116, 975, 1167, 1328, 1459, 1836 |
| BveI | ACCTGC | 2 cut(s) | 750, 1975 |
| CfoI | GCGC | 5 cut(s) | 523, 803, 866, 1084, 1584 |
| Cfr10I | RCCGGY | 2 cut(s) | 182, 764 |
| Cfr42I | CCGCGG | 1 cut(s) | 1621 |
| CseI | GACGC | 4 cut(s) | 332, 357, 1947, 1991 |
| Csp6I | GTAC | 3 cut(s) | 252, 895, 1215 |
| CspAI | ACCGGT | 1 cut(s) | 764 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 924, 959 |
| CviQI | GTAC | 3 cut(s) | 252, 895, 1215 |
| DdeI | CTNAG | 7 cut(s) | 915, 1221, 1416, 1451, 1812, 1827, 2019 |
| DinI | GGCGCC | 1 cut(s) | 1583 |
| DpnI | GATC | 8 cut(s) | 20, 410, 558, 786, 933, 1707, 2122, 2127 |
| DpnII | GATC | 8 cut(s) | 18, 408, 556, 784, 931, 1705, 2120, 2125 |
| DraI | TTTAAA | 1 cut(s) | 1852 |
| EaeI | YGGCCR | 3 cut(s) | 184, 1635, 2070 |
| EagI | CGGCCG | 1 cut(s) | 184 |
| Eam1104I | CTCTTC | 5 cut(s) | 1263, 1304, 1436, 1811, 2163 |
| EarI | CTCTTC | 5 cut(s) | 1263, 1304, 1436, 1811, 2163 |
| EciI | GGCGGA | 2 cut(s) | 336, 1099 |
| EclXI | CGGCCG | 1 cut(s) | 184 |
| Eco130I | CCWWGG | 7 cut(s) | 158, 610, 692, 1094, 1152, 1590, 1608 |
| Eco147I | AGGCCT | 1 cut(s) | 1201 |
| Eco24I | GRGCYC | 4 cut(s) | 1128, 1300, 1462, 2108 |
| Eco31I | GGTCTC | 1 cut(s) | 1535 |
| Eco47I | GGWCC | 4 cut(s) | 275, 701, 1072, 1648 |
| Eco52I | CGGCCG | 1 cut(s) | 184 |
| Eco57I | CTGAAG | 6 cut(s) | 374, 1217, 1352, 1532, 1721, 1727 |
| Eco88I | CYCGRG | 4 cut(s) | 22, 1652, 2082, 2186 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1589, 1826 |
| EcoO109I | RGGNCCY | 6 cut(s) | 701, 1072, 1125, 1296, 1324, 1648 |
| EcoRI | GAATTC | 1 cut(s) | 1400 |
| EcoRII | CCWGG | 6 cut(s) | 325, 417, 645, 906, 1970, 2116 |
| EcoT14I | CCWWGG | 7 cut(s) | 158, 610, 692, 1094, 1152, 1590, 1608 |
| EcoT22I | ATGCAT | 1 cut(s) | 844 |
| EcoT38I | GRGCYC | 4 cut(s) | 1128, 1300, 1462, 2108 |
| EgeI | GGCGCC | 1 cut(s) | 1583 |
| EheI | GGCGCC | 1 cut(s) | 1583 |
| ErhI | CCWWGG | 7 cut(s) | 158, 610, 692, 1094, 1152, 1590, 1608 |
| FaqI | GGGAC | 4 cut(s) | 714, 1058, 1661, 1945 |
| FauI | CCCGC | 9 cut(s) | 303, 376, 441, 1165, 1307, 1413, 1625, 1638, 1740 |
| FbaI | TGATCA | 1 cut(s) | 2125 |
| FblI | GTMKAC | 1 cut(s) | 597 |
| FokI | GGATG | 2 cut(s) | 371, 1625 |
| FriOI | GRGCYC | 4 cut(s) | 1128, 1300, 1462, 2108 |
| FspBI | CTAG | 6 cut(s) | 6, 131, 201, 903, 1679, 2138 |
| FspI | TGCGCA | 1 cut(s) | 865 |
| GlaI | GCGC | 5 cut(s) | 522, 802, 865, 1083, 1583 |
| GsaI | CCCAGC | 2 cut(s) | 1120, 2145 |
| GsuI | CTGGAG | 6 cut(s) | 291, 905, 1199, 1517, 1826, 2180 |
| HaeII | RGCGCY | 2 cut(s) | 524, 1585 |
| HapII | CCGG | 9 cut(s) | 39, 183, 191, 319, 546, 765, 818, 1801, 2006 |
| HgaI | GACGC | 4 cut(s) | 332, 357, 1947, 1991 |
| HhaI | GCGC | 5 cut(s) | 523, 803, 866, 1084, 1584 |
| Hin1I | GRCGYC | 1 cut(s) | 1582 |
| Hin6I | GCGC | 5 cut(s) | 521, 801, 864, 1082, 1582 |
| HinP1I | GCGC | 5 cut(s) | 521, 801, 864, 1082, 1582 |
| HincII | GTYRAC | 2 cut(s) | 262, 795 |
| HindII | GTYRAC | 2 cut(s) | 262, 795 |
| HpaII | CCGG | 9 cut(s) | 39, 183, 191, 319, 546, 765, 818, 1801, 2006 |
| HphI | GGTGA | 7 cut(s) | 640, 754, 1348, 1802, 1903, 1963, 2009 |
| Hpy166II | GTNNAC | 5 cut(s) | 262, 400, 598, 631, 795 |
| Hpy188III | TCNNGA | 9 cut(s) | 22, 191, 884, 1376, 1405, 1463, 1534, 2006, 2186 |
| Hpy8I | GTNNAC | 5 cut(s) | 262, 400, 598, 631, 795 |
| Hpy99I | CGWCG | 3 cut(s) | 14, 373, 1013 |
| HpyAV | CCTTC | 9 cut(s) | 349, 714, 1126, 1327, 1507, 1520, 1696, 1880, 1927 |
| HpyCH4III | ACNGT | 6 cut(s) | 120, 286, 565, 792, 1013, 1840 |
| HpyCH4IV | ACGT | 2 cut(s) | 48, 600 |
| HpyCH4V | TGCA | 6 cut(s) | 107, 400, 580, 842, 1192, 2060 |
| HpyF3I | CTNAG | 7 cut(s) | 915, 1221, 1416, 1451, 1812, 1827, 2019 |
| HpySE526I | ACGT | 2 cut(s) | 48, 600 |
| Hsp92I | GRCGYC | 1 cut(s) | 1582 |
| HspAI | GCGC | 5 cut(s) | 521, 801, 864, 1082, 1582 |
| KasI | GGCGCC | 1 cut(s) | 1581 |
| Kpn2I | TCCGGA | 2 cut(s) | 190, 2005 |
| KpnI | GGTACC | 1 cut(s) | 1218 |
| KroI | GCCGGC | 1 cut(s) | 182 |
| KroNI | GCCGGC | 1 cut(s) | 184 |
| Ksp22I | TGATCA | 1 cut(s) | 2125 |
| KspI | CCGCGG | 1 cut(s) | 1621 |
| Kzo9I | GATC | 8 cut(s) | 18, 408, 556, 784, 931, 1705, 2120, 2125 |
| LguI | GCTCTTC | 1 cut(s) | 1811 |
| LmnI | GCTCC | 7 cut(s) | 772, 1010, 1065, 1180, 1540, 1937, 2188 |
| Lsp1109I | GCAGC | 8 cut(s) | 309, 327, 564, 960, 963, 1066, 1891, 2167 |
| LweI | GCATC | 5 cut(s) | 363, 829, 972, 1179, 1603 |
| MaeI | CTAG | 6 cut(s) | 6, 131, 201, 903, 1679, 2138 |
| MaeII | ACGT | 2 cut(s) | 48, 600 |
| MaeIII | GTNAC | 7 cut(s) | 295, 424, 737, 821, 1162, 1336, 1482 |
| MalI | GATC | 8 cut(s) | 20, 410, 558, 786, 933, 1707, 2122, 2127 |
| MbiI | CCGCTC | 6 cut(s) | 834, 1371, 1420, 1543, 1579, 1645 |
| MboI | GATC | 8 cut(s) | 18, 408, 556, 784, 931, 1705, 2120, 2125 |
| MfeI | CAATTG | 1 cut(s) | 1193 |
| MflI | RGATCY | 3 cut(s) | 18, 556, 2120 |
| MhlI | GDGCHC | 7 cut(s) | 402, 657, 1128, 1149, 1300, 1462, 2108 |
| MlsI | TGGCCA | 1 cut(s) | 1637 |
| MluCI | AATT | 9 cut(s) | 453, 590, 663, 670, 1193, 1400, 1424, 1496, 2191 |
| MluNI | TGGCCA | 1 cut(s) | 1637 |
| Mly113I | GGCGCC | 1 cut(s) | 1582 |
| MmeI | TCCRAC | 4 cut(s) | 584, 1032, 1764, 2070 |
| Mox20I | TGGCCA | 1 cut(s) | 1637 |
| Mph1103I | ATGCAT | 1 cut(s) | 844 |
| MroI | TCCGGA | 2 cut(s) | 190, 2005 |
| MroNI | GCCGGC | 1 cut(s) | 182 |
| MscI | TGGCCA | 1 cut(s) | 1637 |
| MseI | TTAA | 3 cut(s) | 662, 873, 1851 |
| Msp20I | TGGCCA | 1 cut(s) | 1637 |
| MspA1I | CMGCKG | 8 cut(s) | 352, 383, 1211, 1620, 1747, 2023, 2158, 2180 |
| MspI | CCGG | 9 cut(s) | 39, 183, 191, 319, 546, 765, 818, 1801, 2006 |
| MspR9I | CCNGG | 9 cut(s) | 40, 327, 419, 647, 819, 908, 1801, 1972, 2118 |
| MssI | GTTTAAAC | 1 cut(s) | 1852 |
| MunI | CAATTG | 1 cut(s) | 1193 |
| MvaI | CCWGG | 6 cut(s) | 327, 419, 647, 908, 1972, 2118 |
| MvnI | CGCG | 3 cut(s) | 246, 801, 1620 |
| NaeI | GCCGGC | 1 cut(s) | 184 |
| NarI | GGCGCC | 1 cut(s) | 1582 |
| NciI | CCSGG | 3 cut(s) | 40, 819, 1801 |
| NcoI | CCATGG | 1 cut(s) | 1608 |
| NdeII | GATC | 8 cut(s) | 18, 408, 556, 784, 931, 1705, 2120, 2125 |
| NgoMIV | GCCGGC | 1 cut(s) | 182 |
| NheI | GCTAGC | 2 cut(s) | 5, 902 |
| NmeAIII | GCCGAG | 1 cut(s) | 2127 |
| NmuCI | GTSAC | 5 cut(s) | 295, 424, 1162, 1336, 1482 |
| NsbI | TGCGCA | 1 cut(s) | 865 |
| NsiI | ATGCAT | 1 cut(s) | 844 |
| NspI | RCATGY | 3 cut(s) | 392, 895, 1924 |
| PaeR7I | CTCGAG | 2 cut(s) | 22, 1652 |
| PceI | AGGCCT | 1 cut(s) | 1201 |
| PciI | ACATGT | 1 cut(s) | 1920 |
| PciSI | GCTCTTC | 1 cut(s) | 1811 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 92, 1017 |
| PdiI | GCCGGC | 1 cut(s) | 184 |
| PfeI | GAWTC | 6 cut(s) | 35, 680, 733, 809, 1738, 2015 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 159, 457, 1947 |
| PfoI | TCCNGGA | 1 cut(s) | 2116 |
| PinAI | ACCGGT | 1 cut(s) | 764 |
| PluTI | GGCGCC | 1 cut(s) | 1585 |
| PmeI | GTTTAAAC | 1 cut(s) | 1852 |
| Ppu21I | YACGTR | 1 cut(s) | 601 |
| PpuMI | RGGWCCY | 3 cut(s) | 701, 1072, 1648 |
| PscI | ACATGT | 1 cut(s) | 1920 |
| PshAI | GACNNNNGTC | 2 cut(s) | 753, 926 |
| PshBI | ATTAAT | 1 cut(s) | 873 |
| Psp5II | RGGWCCY | 3 cut(s) | 701, 1072, 1648 |
| Psp6I | CCWGG | 6 cut(s) | 325, 417, 645, 906, 1970, 2116 |
| PspFI | CCCAGC | 2 cut(s) | 1116, 2141 |
| PspGI | CCWGG | 6 cut(s) | 325, 417, 645, 906, 1970, 2116 |
| PspOMI | GGGCCC | 2 cut(s) | 1124, 1296 |
| PspPPI | RGGWCCY | 3 cut(s) | 701, 1072, 1648 |
| PspXI | VCTCGAGB | 1 cut(s) | 1652 |
| PsuI | RGATCY | 3 cut(s) | 18, 556, 2120 |
| RsaI | GTAC | 3 cut(s) | 253, 896, 1216 |
| RsaNI | GTAC | 3 cut(s) | 252, 895, 1215 |
| SacII | CCGCGG | 1 cut(s) | 1621 |
| SapI | GCTCTTC | 1 cut(s) | 1811 |
| SaqAI | TTAA | 3 cut(s) | 662, 873, 1851 |
| Sau3AI | GATC | 8 cut(s) | 18, 408, 556, 784, 931, 1705, 2120, 2125 |
| ScrFI | CCNGG | 9 cut(s) | 40, 327, 419, 647, 819, 908, 1801, 1972, 2118 |
| SduI | GDGCHC | 7 cut(s) | 402, 657, 1128, 1149, 1300, 1462, 2108 |
| SfaNI | GCATC | 5 cut(s) | 363, 829, 972, 1179, 1603 |
| SfcI | CTRYAG | 3 cut(s) | 146, 1839, 1860 |
| SfoI | GGCGCC | 1 cut(s) | 1583 |
| Sfr274I | CTCGAG | 2 cut(s) | 22, 1652 |
| Sfr303I | CCGCGG | 1 cut(s) | 1621 |
| SgrBI | CCGCGG | 1 cut(s) | 1621 |
| SinI | GGWCC | 4 cut(s) | 275, 701, 1072, 1648 |
| SlaI | CTCGAG | 2 cut(s) | 22, 1652 |
| SmlI | CTYRAG | 4 cut(s) | 22, 634, 1461, 1652 |
| SmoI | CTYRAG | 4 cut(s) | 22, 634, 1461, 1652 |
| Sse9I | AATT | 9 cut(s) | 453, 590, 663, 670, 1193, 1400, 1424, 1496, 2191 |
| SseBI | AGGCCT | 1 cut(s) | 1201 |
| SspDI | GGCGCC | 1 cut(s) | 1581 |
| SspMI | CTAG | 6 cut(s) | 6, 131, 201, 903, 1679, 2138 |
| StuI | AGGCCT | 1 cut(s) | 1201 |
| StyD4I | CCNGG | 9 cut(s) | 38, 325, 417, 645, 817, 906, 1799, 1970, 2116 |
| StyI | CCWWGG | 7 cut(s) | 158, 610, 692, 1094, 1152, 1590, 1608 |
| TaaI | ACNGT | 6 cut(s) | 120, 286, 565, 792, 1013, 1840 |
| TaiI | ACGT | 2 cut(s) | 51, 603 |
| TaqI | TCGA | 8 cut(s) | 23, 371, 747, 783, 930, 1260, 1524, 1653 |
| TaqII | GACCGA | 2 cut(s) | 292, 1424 |
| TasI | AATT | 9 cut(s) | 453, 590, 663, 670, 1193, 1400, 1424, 1496, 2191 |
| TfiI | GAWTC | 6 cut(s) | 35, 680, 733, 809, 1738, 2015 |
| Tru1I | TTAA | 3 cut(s) | 662, 873, 1851 |
| Tru9I | TTAA | 3 cut(s) | 662, 873, 1851 |
| TscAI | CASTG | 7 cut(s) | 34, 123, 975, 1167, 1335, 1459, 1843 |
| TseFI | GTSAC | 5 cut(s) | 295, 424, 1162, 1336, 1482 |
| TseI | GCWGC | 8 cut(s) | 322, 340, 577, 973, 976, 1079, 1904, 2180 |
| Tsp45I | GTSAC | 5 cut(s) | 295, 424, 1162, 1336, 1482 |
| TspDTI | ATGAA | 3 cut(s) | 1278, 1790, 2056 |
| TspGWI | ACGGA | 2 cut(s) | 84, 743 |
| TspRI | CASTG | 7 cut(s) | 34, 123, 975, 1167, 1335, 1459, 1843 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 159, 457, 1947 |
| VneI | GTGCAC | 1 cut(s) | 398 |
| VpaK11BI | GGWCC | 4 cut(s) | 275, 701, 1072, 1648 |
| VspI | ATTAAT | 1 cut(s) | 873 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1589, 1826 |
| XapI | RAATTY | 5 cut(s) | 453, 670, 1400, 1424, 1496 |
| XceI | RCATGY | 3 cut(s) | 392, 895, 1924 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 617, 723 |
| XhoI | CTCGAG | 2 cut(s) | 22, 1652 |
| XmiI | GTMKAC | 1 cut(s) | 597 |
| XspI | CTAG | 6 cut(s) | 6, 131, 201, 903, 1679, 2138 |
| Zsp2I | ATGCAT | 1 cut(s) | 844 |