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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 95 |
| AccB1I | GGYRCC | 3 cut(s) | 56, 95, 157 |
| AccBSI | CCGCTC | 2 cut(s) | 496, 631 |
| AccII | CGCG | 2 cut(s) | 163, 471 |
| AclWI | GGATC | 1 cut(s) | 564 |
| AcuI | CTGAAG | 1 cut(s) | 262 |
| AcyI | GRCGYC | 2 cut(s) | 158, 332 |
| AfaI | GTAC | 2 cut(s) | 40, 97 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 346, 442, 495, 693 |
| AgsI | TTSAA | 2 cut(s) | 481, 703 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 520 |
| AjnI | CCWGG | 1 cut(s) | 324 |
| AluBI | AGCT | 7 cut(s) | 282, 310, 361, 415, 508, 529, 545 |
| AluI | AGCT | 7 cut(s) | 282, 310, 361, 415, 508, 529, 545 |
| Alw26I | GTCTC | 2 cut(s) | 217, 386 |
| AlwI | GGATC | 1 cut(s) | 564 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 361, 436 |
| Ama87I | CYCGRG | 1 cut(s) | 345 |
| AoxI | GGCC | 6 cut(s) | 75, 125, 149, 402, 433, 681 |
| ApaI | GGGCCC | 1 cut(s) | 79 |
| ApeKI | GCWGC | 5 cut(s) | 6, 165, 355, 358, 672 |
| Asp718I | GGTACC | 1 cut(s) | 95 |
| AspLEI | GCGC | 3 cut(s) | 160, 165, 252 |
| AspS9I | GGNCC | 5 cut(s) | 33, 75, 76, 433, 499 |
| AsuC2I | CCSGG | 3 cut(s) | 346, 347, 652 |
| AvaI | CYCGRG | 1 cut(s) | 345 |
| AvaII | GGWCC | 2 cut(s) | 33, 499 |
| BaeGI | GKGCMC | 1 cut(s) | 79 |
| BanI | GGYRCC | 3 cut(s) | 56, 95, 157 |
| BanII | GRGCYC | 1 cut(s) | 79 |
| BbvCI | CCTCAGC | 2 cut(s) | 362, 459 |
| BbvI | GCAGC | 4 cut(s) | 152, 367, 370, 684 |
| BccI | CCATC | 1 cut(s) | 497 |
| BciT130I | CCWGG | 1 cut(s) | 326 |
| BcnI | CCSGG | 3 cut(s) | 346, 347, 652 |
| BcoDI | GTCTC | 2 cut(s) | 217, 386 |
| BfaI | CTAG | 2 cut(s) | 336, 395 |
| BfoI | RGCGCY | 1 cut(s) | 161 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 656 |
| BglII | AGATCT | 1 cut(s) | 588 |
| BlpI | GCTNAGC | 1 cut(s) | 351 |
| Bme1390I | CCNGG | 4 cut(s) | 326, 346, 347, 652 |
| Bme18I | GGWCC | 2 cut(s) | 33, 499 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 520 |
| BmeT110I | CYCGRG | 1 cut(s) | 345 |
| BmgT120I | GGNCC | 5 cut(s) | 33, 75, 76, 433, 499 |
| BmiI | GGNNCC | 8 cut(s) | 58, 77, 78, 97, 109, 132, 159, 500 |
| BmrFI | CCNGG | 4 cut(s) | 326, 346, 347, 652 |
| BmsI | GCATC | 1 cut(s) | 454 |
| BpmI | CTGGAG | 3 cut(s) | 347, 368, 551 |
| Bpu10I | CCTNAGC | 2 cut(s) | 362, 459 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 351 |
| BpuEI | CTTGAG | 1 cut(s) | 488 |
| BpuMI | CCSGG | 3 cut(s) | 346, 347, 652 |
| BsaHI | GRCGYC | 2 cut(s) | 158, 332 |
| BsaI | GGTCTC | 2 cut(s) | 217, 386 |
| BsaJI | CCNNGG | 6 cut(s) | 344, 345, 441, 469, 618, 678 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 346, 442, 495, 693 |
| Bse1I | ACTGG | 3 cut(s) | 35, 111, 268 |
| BseBI | CCWGG | 1 cut(s) | 326 |
| BseDI | CCNNGG | 6 cut(s) | 344, 345, 441, 469, 618, 678 |
| BseGI | GGATG | 2 cut(s) | 427, 469 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 346, 442, 495, 693 |
| BseMII | CTCAG | 3 cut(s) | 342, 353, 450 |
| BseNI | ACTGG | 3 cut(s) | 35, 111, 268 |
| BseSI | GKGCMC | 1 cut(s) | 79 |
| BseXI | GCAGC | 4 cut(s) | 152, 367, 370, 684 |
| BseYI | CCCAGC | 1 cut(s) | 525 |
| Bsh1236I | CGCG | 2 cut(s) | 163, 471 |
| BshFI | GGCC | 6 cut(s) | 77, 127, 151, 404, 435, 683 |
| BshNI | GGYRCC | 3 cut(s) | 56, 95, 157 |
| BsiHKCI | CYCGRG | 1 cut(s) | 345 |
| BsiSI | CCGG | 3 cut(s) | 128, 346, 652 |
| BslFI | GGGAC | 2 cut(s) | 508, 512 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 346, 442, 495, 693 |
| BsmAI | GTCTC | 2 cut(s) | 217, 386 |
| BsmFI | GGGAC | 2 cut(s) | 508, 512 |
| BsnI | GGCC | 6 cut(s) | 77, 127, 151, 404, 435, 683 |
| Bso31I | GGTCTC | 2 cut(s) | 217, 386 |
| BsoBI | CYCGRG | 1 cut(s) | 345 |
| Bsp120I | GGGCCC | 1 cut(s) | 75 |
| Bsp1286I | GDGCHC | 1 cut(s) | 79 |
| Bsp1407I | TGTACA | 1 cut(s) | 38 |
| Bsp143I | GATC | 2 cut(s) | 556, 588 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 351 |
| BspANI | GGCC | 6 cut(s) | 77, 127, 151, 404, 435, 683 |
| BspCNI | CTCAG | 3 cut(s) | 343, 354, 451 |
| BspFNI | CGCG | 2 cut(s) | 163, 471 |
| BspLI | GGNNCC | 8 cut(s) | 58, 77, 78, 97, 109, 132, 159, 500 |
| BspPI | GGATC | 1 cut(s) | 564 |
| BspT107I | GGYRCC | 3 cut(s) | 56, 95, 157 |
| BspTNI | GGTCTC | 2 cut(s) | 217, 386 |
| BsrBI | CCGCTC | 2 cut(s) | 496, 631 |
| BsrGI | TGTACA | 1 cut(s) | 38 |
| BsrI | ACTGG | 3 cut(s) | 35, 111, 268 |
| BssECI | CCNNGG | 6 cut(s) | 344, 345, 441, 469, 618, 678 |
| BssMI | GATC | 2 cut(s) | 556, 588 |
| BssNI | GRCGYC | 2 cut(s) | 158, 332 |
| BssT1I | CCWWGG | 1 cut(s) | 441 |
| Bst2UI | CCWGG | 1 cut(s) | 326 |
| Bst4CI | ACNGT | 3 cut(s) | 380, 518, 691 |
| Bst6I | CTCTTC | 1 cut(s) | 363 |
| BstACI | GRCGYC | 2 cut(s) | 158, 332 |
| BstAUI | TGTACA | 1 cut(s) | 38 |
| BstC8I | GCNNGC | 5 cut(s) | 11, 61, 125, 341, 469 |
| BstDEI | CTNAG | 3 cut(s) | 351, 362, 459 |
| BstDSI | CCRYGG | 2 cut(s) | 469, 618 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 440 |
| BstF5I | GGATG | 2 cut(s) | 427, 469 |
| BstFNI | CGCG | 2 cut(s) | 163, 471 |
| BstH2I | RGCGCY | 1 cut(s) | 161 |
| BstHHI | GCGC | 3 cut(s) | 160, 165, 252 |
| BstKTI | GATC | 2 cut(s) | 559, 591 |
| BstMAI | GTCTC | 2 cut(s) | 217, 386 |
| BstMBI | GATC | 2 cut(s) | 556, 588 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 133, 157, 174, 464, 656 |
| BstNI | CCWGG | 1 cut(s) | 326 |
| BstSCI | CCNGG | 4 cut(s) | 324, 344, 345, 650 |
| BstSLI | GKGCMC | 1 cut(s) | 79 |
| BstUI | CGCG | 2 cut(s) | 163, 471 |
| BstV1I | GCAGC | 4 cut(s) | 152, 367, 370, 684 |
| BstX2I | RGATCY | 1 cut(s) | 588 |
| BstYI | RGATCY | 1 cut(s) | 588 |
| BsuRI | GGCC | 6 cut(s) | 77, 127, 151, 404, 435, 683 |
| BtgI | CCRYGG | 2 cut(s) | 469, 618 |
| BtsCI | GGATG | 2 cut(s) | 427, 469 |
| BtsIMutI | CAGTG | 1 cut(s) | 696 |
| Cac8I | GCNNGC | 5 cut(s) | 11, 61, 125, 341, 469 |
| CaiI | CAGNNNCTG | 2 cut(s) | 361, 436 |
| CfoI | GCGC | 3 cut(s) | 160, 165, 252 |
| Cfr13I | GGNCC | 5 cut(s) | 33, 75, 76, 433, 499 |
| Cfr42I | CCGCGG | 1 cut(s) | 472 |
| Cfr9I | CCCGGG | 1 cut(s) | 345 |
| CseI | GACGC | 2 cut(s) | 321, 464 |
| Csp6I | GTAC | 2 cut(s) | 39, 96 |
| CviAII | CATG | 1 cut(s) | 314 |
| CviQI | GTAC | 2 cut(s) | 39, 96 |
| DdeI | CTNAG | 3 cut(s) | 351, 362, 459 |
| DinI | GGCGCC | 1 cut(s) | 159 |
| DpnI | GATC | 2 cut(s) | 558, 590 |
| DpnII | GATC | 2 cut(s) | 556, 588 |
| DriI | GACNNNNNGTC | 1 cut(s) | 520 |
| Eam1104I | CTCTTC | 1 cut(s) | 363 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 520 |
| EarI | CTCTTC | 1 cut(s) | 363 |
| Eco130I | CCWWGG | 1 cut(s) | 441 |
| Eco147I | AGGCCT | 1 cut(s) | 151 |
| Eco24I | GRGCYC | 1 cut(s) | 79 |
| Eco31I | GGTCTC | 2 cut(s) | 217, 386 |
| Eco47I | GGWCC | 2 cut(s) | 33, 499 |
| Eco57I | CTGAAG | 1 cut(s) | 262 |
| Eco88I | CYCGRG | 1 cut(s) | 345 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 440 |
| EcoO109I | RGGNCCY | 3 cut(s) | 76, 433, 499 |
| EcoRII | CCWGG | 1 cut(s) | 324 |
| EcoT14I | CCWWGG | 1 cut(s) | 441 |
| EcoT38I | GRGCYC | 1 cut(s) | 79 |
| EgeI | GGCGCC | 1 cut(s) | 159 |
| EheI | GGCGCC | 1 cut(s) | 159 |
| ErhI | CCWWGG | 1 cut(s) | 441 |
| FaeI | CATG | 1 cut(s) | 317 |
| FaiI | YATR | 3 cut(s) | 155, 315, 542 |
| FaqI | GGGAC | 2 cut(s) | 508, 512 |
| FatI | CATG | 1 cut(s) | 313 |
| FauI | CCCGC | 4 cut(s) | 6, 476, 489, 591 |
| FokI | GGATG | 2 cut(s) | 434, 476 |
| FriOI | GRGCYC | 1 cut(s) | 79 |
| FspBI | CTAG | 2 cut(s) | 336, 395 |
| GlaI | GCGC | 3 cut(s) | 159, 164, 251 |
| GsaI | CCCAGC | 1 cut(s) | 529 |
| GsuI | CTGGAG | 3 cut(s) | 347, 368, 551 |
| HaeII | RGCGCY | 1 cut(s) | 161 |
| HaeIII | GGCC | 6 cut(s) | 77, 127, 151, 404, 435, 683 |
| HapII | CCGG | 3 cut(s) | 128, 346, 652 |
| HgaI | GACGC | 2 cut(s) | 321, 464 |
| HhaI | GCGC | 3 cut(s) | 160, 165, 252 |
| Hin1I | GRCGYC | 2 cut(s) | 158, 332 |
| Hin1II | CATG | 1 cut(s) | 317 |
| Hin6I | GCGC | 3 cut(s) | 158, 163, 250 |
| HinP1I | GCGC | 3 cut(s) | 158, 163, 250 |
| HinfI | GANTC | 2 cut(s) | 217, 602 |
| HpaII | CCGG | 3 cut(s) | 128, 346, 652 |
| Hpy188I | TCNGA | 1 cut(s) | 222 |
| Hpy188III | TCNNGA | 1 cut(s) | 385 |
| Hpy99I | CGWCG | 1 cut(s) | 458 |
| HpyAV | CCTTC | 5 cut(s) | 77, 98, 286, 298, 649 |
| HpyCH4III | ACNGT | 3 cut(s) | 380, 518, 691 |
| HpyCH4V | TGCA | 2 cut(s) | 123, 168 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 133, 157, 174, 464, 656 |
| HpyF3I | CTNAG | 3 cut(s) | 351, 362, 459 |
| Hsp92I | GRCGYC | 2 cut(s) | 158, 332 |
| Hsp92II | CATG | 1 cut(s) | 317 |
| HspAI | GCGC | 3 cut(s) | 158, 163, 250 |
| KasI | GGCGCC | 1 cut(s) | 157 |
| KpnI | GGTACC | 1 cut(s) | 99 |
| KspI | CCGCGG | 1 cut(s) | 472 |
| Kzo9I | GATC | 2 cut(s) | 556, 588 |
| LmnI | GCTCC | 3 cut(s) | 16, 130, 532 |
| Lsp1109I | GCAGC | 4 cut(s) | 152, 367, 370, 684 |
| LweI | GCATC | 1 cut(s) | 454 |
| MaeI | CTAG | 2 cut(s) | 336, 395 |
| MaeIII | GTNAC | 1 cut(s) | 113 |
| MalI | GATC | 2 cut(s) | 558, 590 |
| MbiI | CCGCTC | 2 cut(s) | 496, 631 |
| MboI | GATC | 2 cut(s) | 556, 588 |
| MboII | GAAGA | 3 cut(s) | 60, 380, 597 |
| MfeI | CAATTG | 1 cut(s) | 560 |
| MflI | RGATCY | 1 cut(s) | 588 |
| MhlI | GDGCHC | 1 cut(s) | 79 |
| MluCI | AATT | 2 cut(s) | 560, 582 |
| Mly113I | GGCGCC | 1 cut(s) | 158 |
| MlyI | GAGTC | 1 cut(s) | 211 |
| MmeI | TCCRAC | 1 cut(s) | 615 |
| MseI | TTAA | 3 cut(s) | 375, 576, 594 |
| MspA1I | CMGCKG | 4 cut(s) | 13, 361, 471, 529 |
| MspI | CCGG | 3 cut(s) | 128, 346, 652 |
| MspR9I | CCNGG | 4 cut(s) | 326, 346, 347, 652 |
| MteI | GCGCNGCGC | 1 cut(s) | 161 |
| MunI | CAATTG | 1 cut(s) | 560 |
| MvaI | CCWGG | 1 cut(s) | 326 |
| MvnI | CGCG | 2 cut(s) | 163, 471 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 133, 157, 174, 464, 656 |
| NarI | GGCGCC | 1 cut(s) | 158 |
| NciI | CCSGG | 3 cut(s) | 346, 347, 652 |
| NdeII | GATC | 2 cut(s) | 556, 588 |
| NlaIII | CATG | 1 cut(s) | 317 |
| NlaIV | GGNNCC | 8 cut(s) | 58, 77, 78, 97, 109, 132, 159, 500 |
| PceI | AGGCCT | 1 cut(s) | 151 |
| PfeI | GAWTC | 1 cut(s) | 602 |
| PflFI | GACNNNGTC | 1 cut(s) | 208 |
| PfoI | TCCNGGA | 1 cut(s) | 324 |
| PleI | GAGTC | 1 cut(s) | 211 |
| PluTI | GGCGCC | 1 cut(s) | 161 |
| PpsI | GAGTC | 1 cut(s) | 211 |
| PpuMI | RGGWCCY | 1 cut(s) | 499 |
| Psp5II | RGGWCCY | 1 cut(s) | 499 |
| Psp6I | CCWGG | 1 cut(s) | 324 |
| PspFI | CCCAGC | 1 cut(s) | 525 |
| PspGI | CCWGG | 1 cut(s) | 324 |
| PspN4I | GGNNCC | 8 cut(s) | 58, 77, 78, 97, 109, 132, 159, 500 |
| PspOMI | GGGCCC | 1 cut(s) | 75 |
| PspPI | GGNCC | 5 cut(s) | 33, 75, 76, 433, 499 |
| PspPPI | RGGWCCY | 1 cut(s) | 499 |
| PstNI | CAGNNNCTG | 2 cut(s) | 361, 436 |
| PsuI | RGATCY | 1 cut(s) | 588 |
| PsyI | GACNNNGTC | 1 cut(s) | 208 |
| PvuII | CAGCTG | 2 cut(s) | 361, 529 |
| RsaI | GTAC | 2 cut(s) | 40, 97 |
| RsaNI | GTAC | 2 cut(s) | 39, 96 |
| SacII | CCGCGG | 1 cut(s) | 472 |
| SaqAI | TTAA | 3 cut(s) | 375, 576, 594 |
| Sau3AI | GATC | 2 cut(s) | 556, 588 |
| Sau96I | GGNCC | 5 cut(s) | 33, 75, 76, 433, 499 |
| SchI | GAGTC | 1 cut(s) | 211 |
| ScrFI | CCNGG | 4 cut(s) | 326, 346, 347, 652 |
| SduI | GDGCHC | 1 cut(s) | 79 |
| SfaNI | GCATC | 1 cut(s) | 454 |
| SfoI | GGCGCC | 1 cut(s) | 159 |
| Sfr303I | CCGCGG | 1 cut(s) | 472 |
| SgrBI | CCGCGG | 1 cut(s) | 472 |
| SinI | GGWCC | 2 cut(s) | 33, 499 |
| SmaI | CCCGGG | 1 cut(s) | 347 |
| SmlI | CTYRAG | 1 cut(s) | 503 |
| SmoI | CTYRAG | 1 cut(s) | 503 |
| Sse9I | AATT | 2 cut(s) | 560, 582 |
| SseBI | AGGCCT | 1 cut(s) | 151 |
| SspDI | GGCGCC | 1 cut(s) | 157 |
| SspMI | CTAG | 2 cut(s) | 336, 395 |
| StuI | AGGCCT | 1 cut(s) | 151 |
| StyD4I | CCNGG | 4 cut(s) | 324, 344, 345, 650 |
| StyI | CCWWGG | 1 cut(s) | 441 |
| TaaI | ACNGT | 3 cut(s) | 380, 518, 691 |
| TaqI | TCGA | 5 cut(s) | 102, 146, 254, 291, 492 |
| TasI | AATT | 2 cut(s) | 560, 582 |
| TatI | WGTACW | 1 cut(s) | 38 |
| TauI | GCSGC | 5 cut(s) | 136, 163, 180, 474, 686 |
| TfiI | GAWTC | 1 cut(s) | 602 |
| Tru1I | TTAA | 3 cut(s) | 375, 576, 594 |
| Tru9I | TTAA | 3 cut(s) | 375, 576, 594 |
| TscAI | CASTG | 1 cut(s) | 696 |
| TseI | GCWGC | 5 cut(s) | 6, 165, 355, 358, 672 |
| TspDTI | ATGAA | 1 cut(s) | 155 |
| TspGWI | ACGGA | 1 cut(s) | 635 |
| TspMI | CCCGGG | 1 cut(s) | 345 |
| TspRI | CASTG | 1 cut(s) | 696 |
| Tth111I | GACNNNGTC | 1 cut(s) | 208 |
| VpaK11BI | GGWCC | 2 cut(s) | 33, 499 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 440 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 412 |
| XmaI | CCCGGG | 1 cut(s) | 345 |
| XspI | CTAG | 2 cut(s) | 336, 395 |