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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 376, 1261 |
| Acc36I | ACCTGC | 1 cut(s) | 246 |
| AccB1I | GGYRCC | 3 cut(s) | 466, 1127, 1340 |
| AccBSI | CCGCTC | 5 cut(s) | 116, 529, 556, 769, 898 |
| AccI | GTMKAC | 3 cut(s) | 128, 234, 269 |
| AccII | CGCG | 6 cut(s) | 54, 139, 141, 589, 810, 1400 |
| AclWI | GGATC | 1 cut(s) | 846 |
| AcoI | YGGCCR | 2 cut(s) | 957, 1020 |
| AcsI | RAATTY | 1 cut(s) | 636 |
| AcuI | CTGAAG | 1 cut(s) | 601 |
| AcvI | CACGTG | 1 cut(s) | 1304 |
| AcyI | GRCGYC | 4 cut(s) | 467, 671, 804, 1148 |
| AfaI | GTAC | 2 cut(s) | 50, 1219 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 12, 336, 691, 834, 951, 1214, 1259, 1294 |
| AflIII | ACRYGT | 2 cut(s) | 856, 1110 |
| AgsI | TTSAA | 4 cut(s) | 1034, 1042, 1117, 1415 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1356 |
| AjiI | CACGTC | 1 cut(s) | 1162 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 518, 550 |
| AluBI | AGCT | 6 cut(s) | 434, 621, 700, 817, 922, 1001 |
| AluI | AGCT | 6 cut(s) | 434, 621, 700, 817, 922, 1001 |
| Alw21I | GWGCWC | 2 cut(s) | 702, 1013 |
| Alw26I | GTCTC | 7 cut(s) | 384, 393, 725, 745, 865, 1184, 1343 |
| AlwI | GGATC | 1 cut(s) | 846 |
| Ama87I | CYCGRG | 4 cut(s) | 684, 1095, 1329, 1368 |
| AoxI | GGCC | 9 cut(s) | 74, 423, 957, 1020, 1092, 1098, 1243, 1332, 1425 |
| ApaI | GGGCCC | 1 cut(s) | 1096 |
| ApeKI | GCWGC | 9 cut(s) | 434, 679, 886, 889, 934, 1016, 1025, 1230, 1418 |
| ApoI | RAATTY | 1 cut(s) | 636 |
| AspLEI | GCGC | 7 cut(s) | 56, 141, 366, 469, 508, 591, 1402 |
| AsuC2I | CCSGG | 6 cut(s) | 685, 686, 1096, 1097, 1330, 1331 |
| AsuHPI | GGTGA | 1 cut(s) | 992 |
| AvaI | CYCGRG | 4 cut(s) | 684, 1095, 1329, 1368 |
| AvaII | GGWCC | 5 cut(s) | 18, 327, 406, 1263, 1374 |
| BaeGI | GKGCMC | 2 cut(s) | 1096, 1203 |
| BalI | TGGCCA | 1 cut(s) | 959 |
| BanI | GGYRCC | 3 cut(s) | 466, 1127, 1340 |
| BanII | GRGCYC | 2 cut(s) | 702, 1096 |
| BbrPI | CACGTG | 1 cut(s) | 1304 |
| BbsI | GAAGAC | 1 cut(s) | 1070 |
| Bbv12I | GWGCWC | 2 cut(s) | 702, 1013 |
| BbvCI | CCTCAGC | 1 cut(s) | 1002 |
| BbvI | GCAGC | 9 cut(s) | 421, 691, 873, 876, 946, 1003, 1012, 1242, 1430 |
| BccI | CCATC | 2 cut(s) | 836, 949 |
| BceAI | ACGGC | 6 cut(s) | 61, 387, 458, 612, 1354, 1394 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1378, 1412 |
| BcnI | CCSGG | 6 cut(s) | 685, 686, 1096, 1097, 1330, 1331 |
| BcoDI | GTCTC | 7 cut(s) | 384, 393, 725, 745, 865, 1184, 1343 |
| BfaI | CTAG | 2 cut(s) | 57, 974 |
| BfmI | CTRYAG | 3 cut(s) | 33, 890, 1212 |
| BfoI | RGCGCY | 2 cut(s) | 367, 470 |
| BfuAI | ACCTGC | 1 cut(s) | 246 |
| Bme1390I | CCNGG | 6 cut(s) | 685, 686, 1096, 1097, 1330, 1331 |
| Bme18I | GGWCC | 5 cut(s) | 18, 327, 406, 1263, 1374 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1356 |
| BmeT110I | CYCGRG | 4 cut(s) | 684, 1095, 1329, 1368 |
| BmgBI | CACGTC | 1 cut(s) | 1162 |
| BmrFI | CCNGG | 6 cut(s) | 685, 686, 1096, 1097, 1330, 1331 |
| BmrI | ACTGGG | 1 cut(s) | 1350 |
| BmuI | ACTGGG | 1 cut(s) | 1350 |
| BpiI | GAAGAC | 1 cut(s) | 1070 |
| BpmI | CTGGAG | 5 cut(s) | 374, 707, 863, 1208, 1337 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1002 |
| BpuEI | CTTGAG | 1 cut(s) | 686 |
| BpuMI | CCSGG | 6 cut(s) | 685, 686, 1096, 1097, 1330, 1331 |
| BsaAI | YACGTR | 1 cut(s) | 1304 |
| BsaHI | GRCGYC | 4 cut(s) | 467, 671, 804, 1148 |
| BsaI | GGTCTC | 2 cut(s) | 725, 1343 |
| BsaJI | CCNNGG | 6 cut(s) | 683, 684, 808, 1095, 1329, 1369 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 390, 420, 518, 548 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 12, 336, 691, 834, 951, 1214, 1259, 1294 |
| Bse118I | RCCGGY | 1 cut(s) | 1168 |
| Bse1I | ACTGG | 6 cut(s) | 220, 234, 311, 759, 780, 1356 |
| BseDI | CCNNGG | 6 cut(s) | 683, 684, 808, 1095, 1329, 1369 |
| BseGI | GGATG | 3 cut(s) | 828, 960, 1249 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 12, 336, 691, 834, 951, 1214, 1259, 1294 |
| BseMII | CTCAG | 4 cut(s) | 87, 366, 986, 993 |
| BseNI | ACTGG | 6 cut(s) | 220, 234, 311, 759, 780, 1356 |
| BseSI | GKGCMC | 2 cut(s) | 1096, 1203 |
| BseX3I | CGGCCG | 1 cut(s) | 1020 |
| BseXI | GCAGC | 9 cut(s) | 421, 691, 873, 876, 946, 1003, 1012, 1242, 1430 |
| BseYI | CCCAGC | 4 cut(s) | 335, 559, 1266, 1278 |
| BsgI | GTGCAG | 1 cut(s) | 953 |
| Bsh1236I | CGCG | 6 cut(s) | 54, 139, 141, 589, 810, 1400 |
| Bsh1285I | CGRYCG | 3 cut(s) | 1023, 1173, 1398 |
| BshFI | GGCC | 9 cut(s) | 76, 425, 959, 1022, 1094, 1100, 1245, 1334, 1427 |
| BshNI | GGYRCC | 3 cut(s) | 466, 1127, 1340 |
| BsiEI | CGRYCG | 3 cut(s) | 1023, 1173, 1398 |
| BsiHKAI | GWGCWC | 2 cut(s) | 702, 1013 |
| BsiHKCI | CYCGRG | 4 cut(s) | 684, 1095, 1329, 1368 |
| BsiSI | CCGG | 7 cut(s) | 367, 685, 832, 1096, 1169, 1308, 1330 |
| BslFI | GGGAC | 5 cut(s) | 31, 132, 786, 1166, 1249 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 12, 336, 691, 834, 951, 1214, 1259, 1294 |
| BsmAI | GTCTC | 7 cut(s) | 384, 393, 725, 745, 865, 1184, 1343 |
| BsmBI | CGTCTC | 1 cut(s) | 393 |
| BsmFI | GGGAC | 5 cut(s) | 31, 132, 786, 1166, 1249 |
| BsmI | GAATGC | 1 cut(s) | 1037 |
| BsnI | GGCC | 9 cut(s) | 76, 425, 959, 1022, 1094, 1100, 1245, 1334, 1427 |
| Bso31I | GGTCTC | 2 cut(s) | 725, 1343 |
| BsoBI | CYCGRG | 4 cut(s) | 684, 1095, 1329, 1368 |
| Bsp120I | GGGCCC | 1 cut(s) | 1092 |
| Bsp1286I | GDGCHC | 4 cut(s) | 702, 1013, 1096, 1203 |
| Bsp143I | GATC | 6 cut(s) | 481, 762, 838, 879, 1378, 1391 |
| BspANI | GGCC | 9 cut(s) | 76, 425, 959, 1022, 1094, 1100, 1245, 1334, 1427 |
| BspCNI | CTCAG | 4 cut(s) | 88, 367, 987, 994 |
| BspFNI | CGCG | 6 cut(s) | 54, 139, 141, 589, 810, 1400 |
| BspHI | TCATGA | 1 cut(s) | 513 |
| BspMAI | CTGCAG | 1 cut(s) | 894 |
| BspMI | ACCTGC | 1 cut(s) | 246 |
| BspPI | GGATC | 1 cut(s) | 846 |
| BspQI | GCTCTTC | 1 cut(s) | 690 |
| BspT107I | GGYRCC | 3 cut(s) | 466, 1127, 1340 |
| BspTNI | GGTCTC | 2 cut(s) | 725, 1343 |
| BsrBI | CCGCTC | 5 cut(s) | 116, 529, 556, 769, 898 |
| BsrFI | RCCGGY | 1 cut(s) | 1168 |
| BsrI | ACTGG | 6 cut(s) | 220, 234, 311, 759, 780, 1356 |
| BssAI | RCCGGY | 1 cut(s) | 1168 |
| BssECI | CCNNGG | 6 cut(s) | 683, 684, 808, 1095, 1329, 1369 |
| BssMI | GATC | 6 cut(s) | 481, 762, 838, 879, 1378, 1391 |
| BssNAI | GTATAC | 1 cut(s) | 129 |
| BssNI | GRCGYC | 4 cut(s) | 467, 671, 804, 1148 |
| Bst1107I | GTATAC | 1 cut(s) | 129 |
| Bst6I | CTCTTC | 3 cut(s) | 141, 387, 690 |
| BstACI | GRCGYC | 4 cut(s) | 467, 671, 804, 1148 |
| BstBAI | YACGTR | 1 cut(s) | 1304 |
| BstDEI | CTNAG | 4 cut(s) | 96, 375, 995, 1002 |
| BstDSI | CCRYGG | 1 cut(s) | 808 |
| BstEII | GGTNACC | 1 cut(s) | 985 |
| BstF5I | GGATG | 3 cut(s) | 828, 960, 1249 |
| BstFNI | CGCG | 6 cut(s) | 54, 139, 141, 589, 810, 1400 |
| BstH2I | RGCGCY | 2 cut(s) | 367, 470 |
| BstHHI | GCGC | 7 cut(s) | 56, 141, 366, 469, 508, 591, 1402 |
| BstKTI | GATC | 6 cut(s) | 484, 765, 841, 882, 1381, 1394 |
| BstMAI | GTCTC | 7 cut(s) | 384, 393, 725, 745, 865, 1184, 1343 |
| BstMBI | GATC | 6 cut(s) | 481, 762, 838, 879, 1378, 1391 |
| BstMCI | CGRYCG | 3 cut(s) | 1023, 1173, 1398 |
| BstNSI | RCATGY | 2 cut(s) | 860, 1114 |
| BstPI | GGTNACC | 1 cut(s) | 985 |
| BstSCI | CCNGG | 6 cut(s) | 683, 684, 1094, 1095, 1328, 1329 |
| BstSFI | CTRYAG | 3 cut(s) | 33, 890, 1212 |
| BstSLI | GKGCMC | 2 cut(s) | 1096, 1203 |
| BstUI | CGCG | 6 cut(s) | 54, 139, 141, 589, 810, 1400 |
| BstV1I | GCAGC | 9 cut(s) | 421, 691, 873, 876, 946, 1003, 1012, 1242, 1430 |
| BstV2I | GAAGAC | 1 cut(s) | 1070 |
| BstX2I | RGATCY | 1 cut(s) | 838 |
| BstYI | RGATCY | 1 cut(s) | 838 |
| BstZ17I | GTATAC | 1 cut(s) | 129 |
| BstZI | CGGCCG | 1 cut(s) | 1020 |
| BsuRI | GGCC | 9 cut(s) | 76, 425, 959, 1022, 1094, 1100, 1245, 1334, 1427 |
| BtgI | CCRYGG | 1 cut(s) | 808 |
| BtrI | CACGTC | 1 cut(s) | 1162 |
| BtsCI | GGATG | 3 cut(s) | 828, 960, 1249 |
| BtsI | GCAGTG | 1 cut(s) | 456 |
| BtsIMutI | CAGTG | 3 cut(s) | 318, 456, 484 |
| BveI | ACCTGC | 1 cut(s) | 246 |
| CciI | TCATGA | 1 cut(s) | 513 |
| CciNI | GCGGCCGC | 1 cut(s) | 1020 |
| CfoI | GCGC | 7 cut(s) | 56, 141, 366, 469, 508, 591, 1402 |
| Cfr10I | RCCGGY | 1 cut(s) | 1168 |
| Cfr42I | CCGCGG | 1 cut(s) | 811 |
| Cfr9I | CCCGGG | 3 cut(s) | 684, 1095, 1329 |
| CseI | GACGC | 7 cut(s) | 16, 145, 426, 660, 812, 1075, 1137 |
| Csp6I | GTAC | 2 cut(s) | 49, 1218 |
| CviAII | CATG | 4 cut(s) | 514, 653, 857, 1111 |
| CviQI | GTAC | 2 cut(s) | 49, 1218 |
| DdeI | CTNAG | 4 cut(s) | 96, 375, 995, 1002 |
| DinI | GGCGCC | 1 cut(s) | 468 |
| DpnI | GATC | 6 cut(s) | 483, 764, 840, 881, 1380, 1393 |
| DpnII | GATC | 6 cut(s) | 481, 762, 838, 879, 1378, 1391 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 376, 1261 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1356 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 376, 1261 |
| EaeI | YGGCCR | 2 cut(s) | 957, 1020 |
| EagI | CGGCCG | 1 cut(s) | 1020 |
| Eam1104I | CTCTTC | 3 cut(s) | 141, 387, 690 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1356 |
| EarI | CTCTTC | 3 cut(s) | 141, 387, 690 |
| EciI | GGCGGA | 9 cut(s) | 194, 196, 398, 470, 752, 773, 800, 934, 1067 |
| Ecl136II | GAGCTC | 1 cut(s) | 700 |
| EclXI | CGGCCG | 1 cut(s) | 1020 |
| Eco24I | GRGCYC | 2 cut(s) | 702, 1096 |
| Eco31I | GGTCTC | 2 cut(s) | 725, 1343 |
| Eco47I | GGWCC | 5 cut(s) | 18, 327, 406, 1263, 1374 |
| Eco52I | CGGCCG | 1 cut(s) | 1020 |
| Eco53kI | GAGCTC | 1 cut(s) | 700 |
| Eco57I | CTGAAG | 1 cut(s) | 601 |
| Eco72I | CACGTG | 1 cut(s) | 1304 |
| Eco88I | CYCGRG | 4 cut(s) | 684, 1095, 1329, 1368 |
| Eco91I | GGTNACC | 1 cut(s) | 985 |
| EcoICRI | GAGCTC | 1 cut(s) | 700 |
| EcoO109I | RGGNCCY | 3 cut(s) | 18, 1092, 1263 |
| EcoO65I | GGTNACC | 1 cut(s) | 985 |
| EcoT38I | GRGCYC | 2 cut(s) | 702, 1096 |
| EgeI | GGCGCC | 1 cut(s) | 468 |
| EheI | GGCGCC | 1 cut(s) | 468 |
| Esp3I | CGTCTC | 1 cut(s) | 393 |
| FaeI | CATG | 4 cut(s) | 517, 656, 860, 1114 |
| FaqI | GGGAC | 5 cut(s) | 31, 132, 786, 1166, 1249 |
| FatI | CATG | 4 cut(s) | 513, 652, 856, 1110 |
| FblI | GTMKAC | 3 cut(s) | 128, 234, 269 |
| FokI | GGATG | 3 cut(s) | 815, 967, 1236 |
| FriOI | GRGCYC | 2 cut(s) | 702, 1096 |
| FspBI | CTAG | 2 cut(s) | 57, 974 |
| GlaI | GCGC | 7 cut(s) | 55, 140, 365, 468, 507, 590, 1401 |
| GsaI | CCCAGC | 4 cut(s) | 339, 563, 1270, 1282 |
| GsuI | CTGGAG | 5 cut(s) | 374, 707, 863, 1208, 1337 |
| HaeII | RGCGCY | 2 cut(s) | 367, 470 |
| HaeIII | GGCC | 9 cut(s) | 76, 425, 959, 1022, 1094, 1100, 1245, 1334, 1427 |
| HapII | CCGG | 7 cut(s) | 367, 685, 832, 1096, 1169, 1308, 1330 |
| HgaI | GACGC | 7 cut(s) | 16, 145, 426, 660, 812, 1075, 1137 |
| HhaI | GCGC | 7 cut(s) | 56, 141, 366, 469, 508, 591, 1402 |
| Hin1I | GRCGYC | 4 cut(s) | 467, 671, 804, 1148 |
| Hin1II | CATG | 4 cut(s) | 517, 656, 860, 1114 |
| Hin6I | GCGC | 7 cut(s) | 54, 139, 364, 467, 506, 589, 1400 |
| HinP1I | GCGC | 7 cut(s) | 54, 139, 364, 467, 506, 589, 1400 |
| HincII | GTYRAC | 1 cut(s) | 235 |
| HindII | GTYRAC | 1 cut(s) | 235 |
| HindIII | AAGCTT | 2 cut(s) | 815, 920 |
| HinfI | GANTC | 5 cut(s) | 165, 266, 377, 488, 941 |
| HpaII | CCGG | 7 cut(s) | 367, 685, 832, 1096, 1169, 1308, 1330 |
| HphI | GGTGA | 1 cut(s) | 992 |
| Hpy166II | GTNNAC | 3 cut(s) | 129, 235, 270 |
| Hpy188I | TCNGA | 7 cut(s) | 97, 170, 716, 1254, 1294, 1362, 1378 |
| Hpy188III | TCNNGA | 6 cut(s) | 383, 485, 514, 724, 842, 1316 |
| Hpy8I | GTNNAC | 3 cut(s) | 129, 235, 270 |
| Hpy99I | CGWCG | 7 cut(s) | 32, 325, 442, 598, 1258, 1294, 1340 |
| HpyCH4IV | ACGT | 2 cut(s) | 1161, 1303 |
| HpyCH4V | TGCA | 3 cut(s) | 449, 892, 934 |
| HpyF3I | CTNAG | 4 cut(s) | 96, 375, 995, 1002 |
| HpySE526I | ACGT | 2 cut(s) | 1161, 1303 |
| Hsp92I | GRCGYC | 4 cut(s) | 467, 671, 804, 1148 |
| Hsp92II | CATG | 4 cut(s) | 517, 656, 860, 1114 |
| HspAI | GCGC | 7 cut(s) | 54, 139, 364, 467, 506, 589, 1400 |
| KasI | GGCGCC | 1 cut(s) | 466 |
| KflI | GGGWCCC | 1 cut(s) | 1263 |
| KspI | CCGCGG | 1 cut(s) | 811 |
| Kzo9I | GATC | 6 cut(s) | 481, 762, 838, 879, 1378, 1391 |
| LguI | GCTCTTC | 1 cut(s) | 690 |
| LmnI | GCTCC | 6 cut(s) | 257, 339, 526, 871, 895, 1008 |
| Lsp1109I | GCAGC | 9 cut(s) | 421, 691, 873, 876, 946, 1003, 1012, 1242, 1430 |
| MaeI | CTAG | 2 cut(s) | 57, 974 |
| MaeII | ACGT | 2 cut(s) | 1161, 1303 |
| MaeIII | GTNAC | 6 cut(s) | 225, 292, 648, 985, 1149, 1299 |
| MalI | GATC | 6 cut(s) | 483, 764, 840, 881, 1380, 1393 |
| MbiI | CCGCTC | 5 cut(s) | 116, 529, 556, 769, 898 |
| MboI | GATC | 6 cut(s) | 481, 762, 838, 879, 1378, 1391 |
| MboII | GAAGA | 6 cut(s) | 80, 155, 158, 404, 707, 1075 |
| MfeI | CAATTG | 1 cut(s) | 600 |
| MflI | RGATCY | 1 cut(s) | 838 |
| MhlI | GDGCHC | 4 cut(s) | 702, 1013, 1096, 1203 |
| MlsI | TGGCCA | 1 cut(s) | 959 |
| MluCI | AATT | 2 cut(s) | 600, 636 |
| MluNI | TGGCCA | 1 cut(s) | 959 |
| Mly113I | GGCGCC | 1 cut(s) | 467 |
| MlyI | GAGTC | 4 cut(s) | 275, 386, 482, 950 |
| MmeI | TCCRAC | 5 cut(s) | 127, 849, 1272, 1277, 1385 |
| Mox20I | TGGCCA | 1 cut(s) | 959 |
| MscI | TGGCCA | 1 cut(s) | 959 |
| MseI | TTAA | 3 cut(s) | 90, 819, 1059 |
| Msp20I | TGGCCA | 1 cut(s) | 959 |
| MspA1I | CMGCKG | 9 cut(s) | 335, 810, 937, 1016, 1025, 1186, 1211, 1233, 1440 |
| MspI | CCGG | 7 cut(s) | 367, 685, 832, 1096, 1169, 1308, 1330 |
| MspR9I | CCNGG | 6 cut(s) | 685, 686, 1096, 1097, 1330, 1331 |
| MunI | CAATTG | 1 cut(s) | 600 |
| Mva1269I | GAATGC | 1 cut(s) | 1037 |
| MvnI | CGCG | 6 cut(s) | 54, 139, 141, 589, 810, 1400 |
| NarI | GGCGCC | 1 cut(s) | 467 |
| NciI | CCSGG | 6 cut(s) | 685, 686, 1096, 1097, 1330, 1331 |
| NdeII | GATC | 6 cut(s) | 481, 762, 838, 879, 1378, 1391 |
| NlaIII | CATG | 4 cut(s) | 517, 656, 860, 1114 |
| NmeAIII | GCCGAG | 2 cut(s) | 523, 602 |
| NmuCI | GTSAC | 3 cut(s) | 292, 1149, 1299 |
| NotI | GCGGCCGC | 1 cut(s) | 1020 |
| NspI | RCATGY | 2 cut(s) | 860, 1114 |
| PagI | TCATGA | 1 cut(s) | 513 |
| PciI | ACATGT | 2 cut(s) | 856, 1110 |
| PciSI | GCTCTTC | 1 cut(s) | 690 |
| PctI | GAATGC | 1 cut(s) | 1037 |
| PfeI | GAWTC | 1 cut(s) | 165 |
| PflFI | GACNNNGTC | 2 cut(s) | 404, 1288 |
| PleI | GAGTC | 4 cut(s) | 274, 385, 482, 949 |
| PluTI | GGCGCC | 1 cut(s) | 470 |
| PmaCI | CACGTG | 1 cut(s) | 1304 |
| PmlI | CACGTG | 1 cut(s) | 1304 |
| PpsI | GAGTC | 4 cut(s) | 274, 385, 482, 949 |
| Ppu21I | YACGTR | 1 cut(s) | 1304 |
| PpuMI | RGGWCCY | 2 cut(s) | 18, 1263 |
| PscI | ACATGT | 2 cut(s) | 856, 1110 |
| Psp124BI | GAGCTC | 1 cut(s) | 702 |
| Psp5II | RGGWCCY | 2 cut(s) | 18, 1263 |
| PspCI | CACGTG | 1 cut(s) | 1304 |
| PspEI | GGTNACC | 1 cut(s) | 985 |
| PspFI | CCCAGC | 4 cut(s) | 335, 559, 1266, 1278 |
| PspOMI | GGGCCC | 1 cut(s) | 1092 |
| PspPPI | RGGWCCY | 2 cut(s) | 18, 1263 |
| PstI | CTGCAG | 1 cut(s) | 894 |
| PsuI | RGATCY | 1 cut(s) | 838 |
| PsyI | GACNNNGTC | 2 cut(s) | 404, 1288 |
| RsaI | GTAC | 2 cut(s) | 50, 1219 |
| RsaNI | GTAC | 2 cut(s) | 49, 1218 |
| SacI | GAGCTC | 1 cut(s) | 702 |
| SacII | CCGCGG | 1 cut(s) | 811 |
| SalI | GTCGAC | 1 cut(s) | 233 |
| SapI | GCTCTTC | 1 cut(s) | 690 |
| SaqAI | TTAA | 3 cut(s) | 90, 819, 1059 |
| Sau3AI | GATC | 6 cut(s) | 481, 762, 838, 879, 1378, 1391 |
| SchI | GAGTC | 4 cut(s) | 275, 386, 482, 950 |
| ScrFI | CCNGG | 6 cut(s) | 685, 686, 1096, 1097, 1330, 1331 |
| SduI | GDGCHC | 4 cut(s) | 702, 1013, 1096, 1203 |
| SfcI | CTRYAG | 3 cut(s) | 33, 890, 1212 |
| SfoI | GGCGCC | 1 cut(s) | 468 |
| Sfr303I | CCGCGG | 1 cut(s) | 811 |
| SgrBI | CCGCGG | 1 cut(s) | 811 |
| SinI | GGWCC | 5 cut(s) | 18, 327, 406, 1263, 1374 |
| SmaI | CCCGGG | 3 cut(s) | 686, 1097, 1331 |
| SmlI | CTYRAG | 1 cut(s) | 701 |
| SmoI | CTYRAG | 1 cut(s) | 701 |
| SrfI | GCCCGGGC | 1 cut(s) | 1097 |
| Sse9I | AATT | 2 cut(s) | 600, 636 |
| SspDI | GGCGCC | 1 cut(s) | 466 |
| SspMI | CTAG | 2 cut(s) | 57, 974 |
| SstI | GAGCTC | 1 cut(s) | 702 |
| StyD4I | CCNGG | 6 cut(s) | 683, 684, 1094, 1095, 1328, 1329 |
| TaiI | ACGT | 2 cut(s) | 1164, 1306 |
| TaqI | TCGA | 6 cut(s) | 62, 112, 234, 440, 765, 1173 |
| TaqII | GACCGA | 2 cut(s) | 1088, 1384 |
| TasI | AATT | 2 cut(s) | 600, 636 |
| TfiI | GAWTC | 1 cut(s) | 165 |
| Tru1I | TTAA | 3 cut(s) | 90, 819, 1059 |
| Tru9I | TTAA | 3 cut(s) | 90, 819, 1059 |
| TscAI | CASTG | 3 cut(s) | 318, 456, 484 |
| TseFI | GTSAC | 3 cut(s) | 292, 1149, 1299 |
| TseI | GCWGC | 9 cut(s) | 434, 679, 886, 889, 934, 1016, 1025, 1230, 1418 |
| Tsp45I | GTSAC | 3 cut(s) | 292, 1149, 1299 |
| TspDTI | ATGAA | 5 cut(s) | 81, 502, 533, 867, 980 |
| TspGWI | ACGGA | 1 cut(s) | 1271 |
| TspMI | CCCGGG | 3 cut(s) | 684, 1095, 1329 |
| TspRI | CASTG | 3 cut(s) | 318, 456, 484 |
| Tth111I | GACNNNGTC | 2 cut(s) | 404, 1288 |
| VpaK11BI | GGWCC | 5 cut(s) | 18, 327, 406, 1263, 1374 |
| XapI | RAATTY | 1 cut(s) | 636 |
| XceI | RCATGY | 2 cut(s) | 860, 1114 |
| XmaI | CCCGGG | 3 cut(s) | 684, 1095, 1329 |
| XmiI | GTMKAC | 3 cut(s) | 128, 234, 269 |
| XspI | CTAG | 2 cut(s) | 57, 974 |