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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 724 |
| AatII | GACGTC | 1 cut(s) | 435 |
| Acc36I | ACCTGC | 1 cut(s) | 668 |
| Acc65I | GGTACC | 2 cut(s) | 680, 803 |
| AccB1I | GGYRCC | 2 cut(s) | 680, 803 |
| AccBSI | CCGCTC | 4 cut(s) | 156, 183, 397, 526 |
| AccII | CGCG | 4 cut(s) | 216, 438, 486, 832 |
| AcoI | YGGCCR | 2 cut(s) | 104, 453 |
| AcuI | CTGAAG | 1 cut(s) | 228 |
| AcvI | CACGTG | 1 cut(s) | 767 |
| AcyI | GRCGYC | 2 cut(s) | 299, 432 |
| AfaI | GTAC | 2 cut(s) | 682, 805 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 72, 319, 462, 649, 677, 722, 757 |
| AflIII | ACRYGT | 1 cut(s) | 484 |
| AgsI | TTSAA | 2 cut(s) | 878, 893 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 819 |
| AjnI | CCWGG | 1 cut(s) | 843 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 145, 177 |
| AluBI | AGCT | 5 cut(s) | 248, 328, 361, 445, 674 |
| AluI | AGCT | 5 cut(s) | 248, 328, 361, 445, 674 |
| Alw26I | GTCTC | 4 cut(s) | 353, 493, 514, 806 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 313 |
| Ama87I | CYCGRG | 1 cut(s) | 792 |
| AoxI | GGCC | 3 cut(s) | 104, 453, 706 |
| ApeKI | GCWGC | 4 cut(s) | 307, 514, 517, 674 |
| Asp718I | GGTACC | 2 cut(s) | 680, 803 |
| AspLEI | GCGC | 4 cut(s) | 88, 218, 787, 832 |
| AspS9I | GGNCC | 4 cut(s) | 63, 707, 726, 837 |
| AsuC2I | CCSGG | 3 cut(s) | 73, 793, 794 |
| AsuHPI | GGTGA | 1 cut(s) | 67 |
| AvaI | CYCGRG | 1 cut(s) | 792 |
| AvaII | GGWCC | 3 cut(s) | 63, 726, 837 |
| BaeGI | GKGCMC | 1 cut(s) | 617 |
| BalI | TGGCCA | 1 cut(s) | 455 |
| BanI | GGYRCC | 2 cut(s) | 680, 803 |
| BbrPI | CACGTG | 1 cut(s) | 767 |
| BbvCI | CCTCAGC | 1 cut(s) | 329 |
| BbvI | GCAGC | 4 cut(s) | 319, 501, 504, 661 |
| BccI | CCATC | 2 cut(s) | 179, 597 |
| BceAI | ACGGC | 1 cut(s) | 843 |
| BciT130I | CCWGG | 1 cut(s) | 845 |
| BciVI | GTATCC | 1 cut(s) | 601 |
| BcnI | CCSGG | 3 cut(s) | 73, 793, 794 |
| BcoDI | GTCTC | 4 cut(s) | 353, 493, 514, 806 |
| BfaI | CTAG | 1 cut(s) | 849 |
| BfmI | CTRYAG | 2 cut(s) | 518, 675 |
| BfoI | RGCGCY | 1 cut(s) | 89 |
| BfuAI | ACCTGC | 1 cut(s) | 668 |
| BfuI | GTATCC | 1 cut(s) | 601 |
| Bme1390I | CCNGG | 4 cut(s) | 73, 793, 794, 845 |
| Bme18I | GGWCC | 3 cut(s) | 63, 726, 837 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 819 |
| BmeT110I | CYCGRG | 1 cut(s) | 792 |
| BmgT120I | GGNCC | 4 cut(s) | 63, 707, 726, 837 |
| BmiI | GGNNCC | 7 cut(s) | 42, 77, 682, 727, 728, 805, 838 |
| BmrFI | CCNGG | 4 cut(s) | 73, 793, 794, 845 |
| BmrI | ACTGGG | 1 cut(s) | 813 |
| BmuI | ACTGGG | 1 cut(s) | 813 |
| BpmI | CTGGAG | 3 cut(s) | 110, 335, 800 |
| Bpu10I | CCTNAGC | 1 cut(s) | 329 |
| BpuMI | CCSGG | 3 cut(s) | 73, 793, 794 |
| Bsa29I | ATCGAT | 1 cut(s) | 857 |
| BsaAI | YACGTR | 1 cut(s) | 767 |
| BsaHI | GRCGYC | 2 cut(s) | 299, 432 |
| BsaI | GGTCTC | 3 cut(s) | 353, 514, 806 |
| BsaJI | CCNNGG | 3 cut(s) | 343, 436, 792 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 126, 145, 156, 175 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 72, 319, 462, 649, 677, 722, 757 |
| Bse118I | RCCGGY | 4 cut(s) | 10, 106, 579, 628 |
| Bse1I | ACTGG | 3 cut(s) | 47, 387, 819 |
| BseBI | CCWGG | 1 cut(s) | 845 |
| BseCI | ATCGAT | 1 cut(s) | 857 |
| BseDI | CCNNGG | 3 cut(s) | 343, 436, 792 |
| BseGI | GGATG | 1 cut(s) | 712 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 72, 319, 462, 649, 677, 722, 757 |
| BseMII | CTCAG | 2 cut(s) | 320, 624 |
| BseNI | ACTGG | 3 cut(s) | 47, 387, 819 |
| BseRI | GAGGAG | 1 cut(s) | 53 |
| BseSI | GKGCMC | 1 cut(s) | 617 |
| BseX3I | CGGCCG | 1 cut(s) | 104 |
| BseXI | GCAGC | 4 cut(s) | 319, 501, 504, 661 |
| BseYI | CCCAGC | 4 cut(s) | 186, 670, 729, 741 |
| Bsh1236I | CGCG | 4 cut(s) | 216, 438, 486, 832 |
| Bsh1285I | CGRYCG | 1 cut(s) | 107 |
| BshFI | GGCC | 3 cut(s) | 106, 455, 708 |
| BshNI | GGYRCC | 2 cut(s) | 680, 803 |
| BshVI | ATCGAT | 1 cut(s) | 857 |
| BsiEI | CGRYCG | 1 cut(s) | 107 |
| BsiHKCI | CYCGRG | 1 cut(s) | 792 |
| BsiSI | CCGG | 9 cut(s) | 11, 72, 107, 580, 598, 629, 647, 771, 793 |
| BslFI | GGGAC | 3 cut(s) | 479, 626, 712 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 72, 319, 462, 649, 677, 722, 757 |
| BsmAI | GTCTC | 4 cut(s) | 353, 493, 514, 806 |
| BsmBI | CGTCTC | 1 cut(s) | 493 |
| BsmFI | GGGAC | 3 cut(s) | 479, 626, 712 |
| BsnI | GGCC | 3 cut(s) | 106, 455, 708 |
| Bso31I | GGTCTC | 3 cut(s) | 353, 514, 806 |
| BsoBI | CYCGRG | 1 cut(s) | 792 |
| Bsp1286I | GDGCHC | 1 cut(s) | 617 |
| Bsp143I | GATC | 1 cut(s) | 854 |
| BspANI | GGCC | 3 cut(s) | 106, 455, 708 |
| BspCNI | CTCAG | 2 cut(s) | 321, 625 |
| BspDI | ATCGAT | 1 cut(s) | 857 |
| BspFNI | CGCG | 4 cut(s) | 216, 438, 486, 832 |
| BspLI | GGNNCC | 7 cut(s) | 42, 77, 682, 727, 728, 805, 838 |
| BspMAI | CTGCAG | 2 cut(s) | 522, 679 |
| BspMI | ACCTGC | 1 cut(s) | 668 |
| BspT107I | GGYRCC | 2 cut(s) | 680, 803 |
| BspTNI | GGTCTC | 3 cut(s) | 353, 514, 806 |
| BsrBI | CCGCTC | 4 cut(s) | 156, 183, 397, 526 |
| BsrFI | RCCGGY | 4 cut(s) | 10, 106, 579, 628 |
| BsrI | ACTGG | 3 cut(s) | 47, 387, 819 |
| BssAI | RCCGGY | 4 cut(s) | 10, 106, 579, 628 |
| BssECI | CCNNGG | 3 cut(s) | 343, 436, 792 |
| BssMI | GATC | 1 cut(s) | 854 |
| BssNI | GRCGYC | 2 cut(s) | 299, 432 |
| BssT1I | CCWWGG | 1 cut(s) | 343 |
| Bst2UI | CCWGG | 1 cut(s) | 845 |
| Bst4CI | ACNGT | 1 cut(s) | 803 |
| Bst6I | CTCTTC | 2 cut(s) | 123, 172 |
| BstACI | GRCGYC | 2 cut(s) | 299, 432 |
| BstBAI | YACGTR | 1 cut(s) | 767 |
| BstC8I | GCNNGC | 8 cut(s) | 84, 108, 181, 363, 443, 617, 630, 666 |
| BstDEI | CTNAG | 2 cut(s) | 329, 633 |
| BstDSI | CCRYGG | 1 cut(s) | 436 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 317 |
| BstF5I | GGATG | 1 cut(s) | 712 |
| BstFNI | CGCG | 4 cut(s) | 216, 438, 486, 832 |
| BstH2I | RGCGCY | 1 cut(s) | 89 |
| BstHHI | GCGC | 4 cut(s) | 88, 218, 787, 832 |
| BstKTI | GATC | 1 cut(s) | 857 |
| BstMAI | GTCTC | 4 cut(s) | 353, 493, 514, 806 |
| BstMBI | GATC | 1 cut(s) | 854 |
| BstMCI | CGRYCG | 1 cut(s) | 107 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 523, 625, 674 |
| BstNI | CCWGG | 1 cut(s) | 845 |
| BstSCI | CCNGG | 4 cut(s) | 71, 791, 792, 843 |
| BstSFI | CTRYAG | 2 cut(s) | 518, 675 |
| BstSLI | GKGCMC | 1 cut(s) | 617 |
| BstUI | CGCG | 4 cut(s) | 216, 438, 486, 832 |
| BstV1I | GCAGC | 4 cut(s) | 319, 501, 504, 661 |
| BstZI | CGGCCG | 1 cut(s) | 104 |
| Bsu15I | ATCGAT | 1 cut(s) | 857 |
| BsuI | GTATCC | 1 cut(s) | 601 |
| BsuRI | GGCC | 3 cut(s) | 106, 455, 708 |
| BsuTUI | ATCGAT | 1 cut(s) | 857 |
| BtgI | CCRYGG | 1 cut(s) | 436 |
| BtsCI | GGATG | 1 cut(s) | 712 |
| BtsIMutI | CAGTG | 1 cut(s) | 54 |
| BveI | ACCTGC | 1 cut(s) | 668 |
| Cac8I | GCNNGC | 8 cut(s) | 84, 108, 181, 363, 443, 617, 630, 666 |
| CaiI | CAGNNNCTG | 1 cut(s) | 313 |
| CfoI | GCGC | 4 cut(s) | 88, 218, 787, 832 |
| Cfr10I | RCCGGY | 4 cut(s) | 10, 106, 579, 628 |
| Cfr13I | GGNCC | 4 cut(s) | 63, 707, 726, 837 |
| Cfr42I | CCGCGG | 1 cut(s) | 439 |
| Cfr9I | CCCGGG | 1 cut(s) | 792 |
| ClaI | ATCGAT | 1 cut(s) | 857 |
| CseI | GACGC | 3 cut(s) | 288, 475, 580 |
| Csp6I | GTAC | 2 cut(s) | 681, 804 |
| CviAII | CATG | 1 cut(s) | 281 |
| CviQI | GTAC | 2 cut(s) | 681, 804 |
| DdeI | CTNAG | 2 cut(s) | 329, 633 |
| DpnI | GATC | 1 cut(s) | 856 |
| DpnII | GATC | 1 cut(s) | 854 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 724 |
| DriI | GACNNNNNGTC | 1 cut(s) | 819 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 724 |
| EaeI | YGGCCR | 2 cut(s) | 104, 453 |
| EagI | CGGCCG | 1 cut(s) | 104 |
| Eam1104I | CTCTTC | 2 cut(s) | 123, 172 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 819 |
| EarI | CTCTTC | 2 cut(s) | 123, 172 |
| EciI | GGCGGA | 5 cut(s) | 29, 380, 401, 428, 812 |
| EclXI | CGGCCG | 1 cut(s) | 104 |
| Eco130I | CCWWGG | 1 cut(s) | 343 |
| Eco31I | GGTCTC | 3 cut(s) | 353, 514, 806 |
| Eco47I | GGWCC | 3 cut(s) | 63, 726, 837 |
| Eco52I | CGGCCG | 1 cut(s) | 104 |
| Eco57I | CTGAAG | 1 cut(s) | 228 |
| Eco72I | CACGTG | 1 cut(s) | 767 |
| Eco88I | CYCGRG | 1 cut(s) | 792 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 317 |
| EcoO109I | RGGNCCY | 1 cut(s) | 726 |
| EcoRII | CCWGG | 1 cut(s) | 843 |
| EcoT14I | CCWWGG | 1 cut(s) | 343 |
| ErhI | CCWWGG | 1 cut(s) | 343 |
| Esp3I | CGTCTC | 1 cut(s) | 493 |
| FaeI | CATG | 1 cut(s) | 284 |
| FaiI | YATR | 8 cut(s) | 177, 211, 282, 287, 426, 557, 623, 809 |
| FaqI | GGGAC | 3 cut(s) | 479, 626, 712 |
| FatI | CATG | 1 cut(s) | 280 |
| FauI | CCCGC | 8 cut(s) | 75, 149, 198, 265, 456, 624, 651, 689 |
| FokI | GGATG | 1 cut(s) | 699 |
| FspBI | CTAG | 1 cut(s) | 849 |
| GlaI | GCGC | 4 cut(s) | 87, 217, 786, 831 |
| GsaI | CCCAGC | 4 cut(s) | 190, 674, 733, 745 |
| GsuI | CTGGAG | 3 cut(s) | 110, 335, 800 |
| HaeII | RGCGCY | 1 cut(s) | 89 |
| HaeIII | GGCC | 3 cut(s) | 106, 455, 708 |
| HapII | CCGG | 9 cut(s) | 11, 72, 107, 580, 598, 629, 647, 771, 793 |
| HgaI | GACGC | 3 cut(s) | 288, 475, 580 |
| HhaI | GCGC | 4 cut(s) | 88, 218, 787, 832 |
| Hin1I | GRCGYC | 2 cut(s) | 299, 432 |
| Hin1II | CATG | 1 cut(s) | 284 |
| Hin6I | GCGC | 4 cut(s) | 86, 216, 785, 830 |
| HinP1I | GCGC | 4 cut(s) | 86, 216, 785, 830 |
| HinfI | GANTC | 2 cut(s) | 113, 736 |
| HpaII | CCGG | 9 cut(s) | 11, 72, 107, 580, 598, 629, 647, 771, 793 |
| HphI | GGTGA | 1 cut(s) | 67 |
| Hpy188I | TCNGA | 4 cut(s) | 717, 757, 825, 841 |
| Hpy188III | TCNNGA | 3 cut(s) | 119, 352, 779 |
| Hpy99I | CGWCG | 6 cut(s) | 105, 225, 304, 721, 757, 829 |
| HpyAV | CCTTC | 3 cut(s) | 142, 252, 716 |
| HpyCH4III | ACNGT | 1 cut(s) | 803 |
| HpyCH4IV | ACGT | 6 cut(s) | 100, 432, 450, 559, 766, 888 |
| HpyCH4V | TGCA | 3 cut(s) | 7, 520, 677 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 523, 625, 674 |
| HpyF3I | CTNAG | 2 cut(s) | 329, 633 |
| HpySE526I | ACGT | 6 cut(s) | 100, 432, 450, 559, 766, 888 |
| Hsp92I | GRCGYC | 2 cut(s) | 299, 432 |
| Hsp92II | CATG | 1 cut(s) | 284 |
| HspAI | GCGC | 4 cut(s) | 86, 216, 785, 830 |
| KflI | GGGWCCC | 1 cut(s) | 726 |
| KpnI | GGTACC | 2 cut(s) | 684, 807 |
| KroI | GCCGGC | 2 cut(s) | 106, 628 |
| KroNI | GCCGGC | 2 cut(s) | 108, 630 |
| KspI | CCGCGG | 1 cut(s) | 439 |
| Kzo9I | GATC | 1 cut(s) | 854 |
| LmnI | GCTCC | 6 cut(s) | 40, 153, 325, 358, 499, 523 |
| Lsp1109I | GCAGC | 4 cut(s) | 319, 501, 504, 661 |
| MaeI | CTAG | 1 cut(s) | 849 |
| MaeII | ACGT | 6 cut(s) | 100, 432, 450, 559, 766, 888 |
| MaeIII | GTNAC | 5 cut(s) | 28, 55, 419, 560, 762 |
| MalI | GATC | 1 cut(s) | 856 |
| MbiI | CCGCTC | 4 cut(s) | 156, 183, 397, 526 |
| MboI | GATC | 1 cut(s) | 854 |
| MboII | GAAGA | 3 cut(s) | 140, 159, 347 |
| MfeI | CAATTG | 1 cut(s) | 227 |
| MhlI | GDGCHC | 1 cut(s) | 617 |
| MlsI | TGGCCA | 1 cut(s) | 455 |
| MluCI | AATT | 1 cut(s) | 227 |
| MluI | ACGCGT | 1 cut(s) | 484 |
| MluNI | TGGCCA | 1 cut(s) | 455 |
| MlyI | GAGTC | 1 cut(s) | 730 |
| MmeI | TCCRAC | 5 cut(s) | 477, 735, 740, 848, 864 |
| Mox20I | TGGCCA | 1 cut(s) | 455 |
| MroNI | GCCGGC | 2 cut(s) | 106, 628 |
| MscI | TGGCCA | 1 cut(s) | 455 |
| Msp20I | TGGCCA | 1 cut(s) | 455 |
| MspA1I | CMGCKG | 4 cut(s) | 82, 438, 674, 696 |
| MspI | CCGG | 9 cut(s) | 11, 72, 107, 580, 598, 629, 647, 771, 793 |
| MspR9I | CCNGG | 4 cut(s) | 73, 793, 794, 845 |
| MunI | CAATTG | 1 cut(s) | 227 |
| MvaI | CCWGG | 1 cut(s) | 845 |
| MvnI | CGCG | 4 cut(s) | 216, 438, 486, 832 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 523, 625, 674 |
| NaeI | GCCGGC | 2 cut(s) | 108, 630 |
| NciI | CCSGG | 3 cut(s) | 73, 793, 794 |
| NdeII | GATC | 1 cut(s) | 854 |
| NgoMIV | GCCGGC | 2 cut(s) | 106, 628 |
| NlaIII | CATG | 1 cut(s) | 284 |
| NlaIV | GGNNCC | 7 cut(s) | 42, 77, 682, 727, 728, 805, 838 |
| NmeAIII | GCCGAG | 1 cut(s) | 229 |
| NmuCI | GTSAC | 5 cut(s) | 28, 55, 419, 560, 762 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 220 |
| PdiI | GCCGGC | 2 cut(s) | 108, 630 |
| PfeI | GAWTC | 1 cut(s) | 113 |
| PflFI | GACNNNGTC | 2 cut(s) | 20, 751 |
| PleI | GAGTC | 1 cut(s) | 730 |
| PmaCI | CACGTG | 1 cut(s) | 767 |
| PmlI | CACGTG | 1 cut(s) | 767 |
| PpsI | GAGTC | 1 cut(s) | 730 |
| Ppu21I | YACGTR | 1 cut(s) | 767 |
| PpuMI | RGGWCCY | 1 cut(s) | 726 |
| Psp5II | RGGWCCY | 1 cut(s) | 726 |
| Psp6I | CCWGG | 1 cut(s) | 843 |
| PspCI | CACGTG | 1 cut(s) | 767 |
| PspFI | CCCAGC | 4 cut(s) | 186, 670, 729, 741 |
| PspGI | CCWGG | 1 cut(s) | 843 |
| PspN4I | GGNNCC | 7 cut(s) | 42, 77, 682, 727, 728, 805, 838 |
| PspPI | GGNCC | 4 cut(s) | 63, 707, 726, 837 |
| PspPPI | RGGWCCY | 1 cut(s) | 726 |
| PstI | CTGCAG | 2 cut(s) | 522, 679 |
| PstNI | CAGNNNCTG | 1 cut(s) | 313 |
| PsyI | GACNNNGTC | 2 cut(s) | 20, 751 |
| PvuII | CAGCTG | 1 cut(s) | 674 |
| RsaI | GTAC | 2 cut(s) | 682, 805 |
| RsaNI | GTAC | 2 cut(s) | 681, 804 |
| SacII | CCGCGG | 1 cut(s) | 439 |
| Sau3AI | GATC | 1 cut(s) | 854 |
| Sau96I | GGNCC | 4 cut(s) | 63, 707, 726, 837 |
| SchI | GAGTC | 1 cut(s) | 730 |
| ScrFI | CCNGG | 4 cut(s) | 73, 793, 794, 845 |
| SduI | GDGCHC | 1 cut(s) | 617 |
| SfcI | CTRYAG | 2 cut(s) | 518, 675 |
| Sfr303I | CCGCGG | 1 cut(s) | 439 |
| SgrBI | CCGCGG | 1 cut(s) | 439 |
| SinI | GGWCC | 3 cut(s) | 63, 726, 837 |
| SmaI | CCCGGG | 1 cut(s) | 794 |
| Sse9I | AATT | 1 cut(s) | 227 |
| SspMI | CTAG | 1 cut(s) | 849 |
| StyD4I | CCNGG | 4 cut(s) | 71, 791, 792, 843 |
| StyI | CCWWGG | 1 cut(s) | 343 |
| TaaI | ACNGT | 1 cut(s) | 803 |
| TaiI | ACGT | 6 cut(s) | 103, 435, 453, 562, 769, 891 |
| TaqI | TCGA | 3 cut(s) | 302, 393, 857 |
| TaqII | GACCGA | 1 cut(s) | 679 |
| TasI | AATT | 1 cut(s) | 227 |
| TauI | GCSGC | 8 cut(s) | 46, 71, 186, 299, 441, 536, 868, 871 |
| TfiI | GAWTC | 1 cut(s) | 113 |
| TscAI | CASTG | 1 cut(s) | 54 |
| TseFI | GTSAC | 5 cut(s) | 28, 55, 419, 560, 762 |
| TseI | GCWGC | 4 cut(s) | 307, 514, 517, 674 |
| Tsp45I | GTSAC | 5 cut(s) | 28, 55, 419, 560, 762 |
| TspDTI | ATGAA | 1 cut(s) | 495 |
| TspGWI | ACGGA | 1 cut(s) | 734 |
| TspMI | CCCGGG | 1 cut(s) | 792 |
| TspRI | CASTG | 1 cut(s) | 54 |
| Tth111I | GACNNNGTC | 2 cut(s) | 20, 751 |
| VpaK11BI | GGWCC | 3 cut(s) | 63, 726, 837 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 317 |
| XmaI | CCCGGG | 1 cut(s) | 792 |
| XspI | CTAG | 1 cut(s) | 849 |
| ZraI | GACGTC | 1 cut(s) | 433 |