No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 682 |
| Acc36I | ACCTGC | 1 cut(s) | 240 |
| AccB1I | GGYRCC | 2 cut(s) | 787, 1538 |
| AccBSI | CCGCTC | 5 cut(s) | 850, 877, 1090, 1219, 1309 |
| AccI | GTMKAC | 1 cut(s) | 228 |
| AccII | CGCG | 6 cut(s) | 150, 244, 282, 827, 910, 1131 |
| AclWI | GGATC | 2 cut(s) | 167, 507 |
| AcoI | YGGCCR | 5 cut(s) | 489, 1146, 1278, 1368, 1431 |
| AcuI | CTGAAG | 2 cut(s) | 922, 1434 |
| AcyI | GRCGYC | 5 cut(s) | 788, 992, 1125, 1539, 1559 |
| AfaI | GTAC | 3 cut(s) | 50, 824, 1423 |
| AfeI | AGCGCT | 1 cut(s) | 514 |
| AflIII | ACRYGT | 2 cut(s) | 1177, 1521 |
| AgsI | TTSAA | 5 cut(s) | 254, 1378, 1445, 1453, 1528 |
| AjiI | CACGTC | 2 cut(s) | 1180, 1573 |
| AleI | CACNNNNGTG | 2 cut(s) | 447, 555 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 839, 871 |
| AluBI | AGCT | 5 cut(s) | 805, 942, 1021, 1138, 1625 |
| AluI | AGCT | 5 cut(s) | 805, 942, 1021, 1138, 1625 |
| Alw21I | GWGCWC | 1 cut(s) | 1023 |
| Alw26I | GTCTC | 9 cut(s) | 377, 444, 640, 690, 699, 1046, 1066, 1186, 1595 |
| AlwI | GGATC | 2 cut(s) | 167, 507 |
| Ama87I | CYCGRG | 1 cut(s) | 1506 |
| Aor51HI | AGCGCT | 1 cut(s) | 514 |
| ApaI | GGGCCC | 2 cut(s) | 730, 1507 |
| ApeKI | GCWGC | 8 cut(s) | 515, 1000, 1210, 1255, 1345, 1427, 1616, 1625 |
| AspLEI | GCGC | 7 cut(s) | 515, 658, 672, 790, 829, 912, 1541 |
| AsuC2I | CCSGG | 8 cut(s) | 264, 308, 380, 496, 1006, 1507, 1508, 1642 |
| AsuHPI | GGTGA | 7 cut(s) | 385, 430, 434, 461, 637, 1313, 1403 |
| AvaI | CYCGRG | 1 cut(s) | 1506 |
| AvaII | GGWCC | 4 cut(s) | 18, 246, 304, 560 |
| AxyI | CCTNAGG | 1 cut(s) | 1406 |
| BaeGI | GKGCMC | 2 cut(s) | 730, 1507 |
| BanI | GGYRCC | 2 cut(s) | 787, 1538 |
| BanII | GRGCYC | 3 cut(s) | 730, 1023, 1507 |
| BbsI | GAAGAC | 2 cut(s) | 770, 1481 |
| Bbv12I | GWGCWC | 1 cut(s) | 1023 |
| BbvI | GCAGC | 8 cut(s) | 502, 1012, 1197, 1267, 1357, 1414, 1603, 1612 |
| BccI | CCATC | 6 cut(s) | 295, 449, 873, 1157, 1270, 1360 |
| BceAI | ACGGC | 7 cut(s) | 504, 620, 693, 717, 779, 933, 1421 |
| BcnI | CCSGG | 8 cut(s) | 264, 308, 380, 496, 1006, 1507, 1508, 1642 |
| BcoDI | GTCTC | 9 cut(s) | 377, 444, 640, 690, 699, 1046, 1066, 1186, 1595 |
| BfaI | CTAG | 1 cut(s) | 806 |
| BfmI | CTRYAG | 3 cut(s) | 33, 1211, 1626 |
| BfoI | RGCGCY | 5 cut(s) | 516, 659, 673, 791, 1542 |
| BfuAI | ACCTGC | 1 cut(s) | 240 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 752 |
| Bme1390I | CCNGG | 8 cut(s) | 264, 308, 380, 496, 1006, 1507, 1508, 1642 |
| Bme18I | GGWCC | 4 cut(s) | 18, 246, 304, 560 |
| BmeT110I | CYCGRG | 1 cut(s) | 1506 |
| BmgBI | CACGTC | 2 cut(s) | 1180, 1573 |
| BmrFI | CCNGG | 8 cut(s) | 264, 308, 380, 496, 1006, 1507, 1508, 1642 |
| BmsI | GCATC | 3 cut(s) | 411, 417, 531 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1607 |
| BpiI | GAAGAC | 2 cut(s) | 770, 1481 |
| BpmI | CTGGAG | 2 cut(s) | 680, 1028 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1022, 1230, 1320 |
| BpuMI | CCSGG | 8 cut(s) | 264, 308, 380, 496, 1006, 1507, 1508, 1642 |
| BsaHI | GRCGYC | 5 cut(s) | 788, 992, 1125, 1539, 1559 |
| BsaI | GGTCTC | 1 cut(s) | 1046 |
| BsaJI | CCNNGG | 8 cut(s) | 345, 405, 445, 495, 1004, 1101, 1129, 1506 |
| BsaWI | WCCGGW | 2 cut(s) | 124, 412 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 323, 353, 696, 726, 839, 869 |
| Bse118I | RCCGGY | 1 cut(s) | 1579 |
| Bse1I | ACTGG | 6 cut(s) | 14, 214, 400, 436, 617, 1080 |
| Bse21I | CCTNAGG | 1 cut(s) | 1406 |
| BseDI | CCNNGG | 8 cut(s) | 345, 405, 445, 495, 1004, 1101, 1129, 1506 |
| BseGI | GGATG | 5 cut(s) | 460, 546, 1149, 1281, 1371 |
| BseMII | CTCAG | 5 cut(s) | 497, 672, 1036, 1397, 1575 |
| BseNI | ACTGG | 6 cut(s) | 14, 214, 400, 436, 617, 1080 |
| BseRI | GAGGAG | 1 cut(s) | 1319 |
| BseSI | GKGCMC | 2 cut(s) | 730, 1507 |
| BseX3I | CGGCCG | 2 cut(s) | 489, 1431 |
| BseXI | GCAGC | 8 cut(s) | 502, 1012, 1197, 1267, 1357, 1414, 1603, 1612 |
| BseYI | CCCAGC | 7 cut(s) | 360, 641, 880, 1294, 1384, 1499, 1621 |
| BsgI | GTGCAG | 3 cut(s) | 491, 1274, 1364 |
| Bsh1236I | CGCG | 6 cut(s) | 150, 244, 282, 827, 910, 1131 |
| Bsh1285I | CGRYCG | 2 cut(s) | 492, 1434 |
| BshNI | GGYRCC | 2 cut(s) | 787, 1538 |
| BsiEI | CGRYCG | 2 cut(s) | 492, 1434 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1023 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1506 |
| BslFI | GGGAC | 4 cut(s) | 31, 493, 1172, 1577 |
| BsmAI | GTCTC | 9 cut(s) | 377, 444, 640, 690, 699, 1046, 1066, 1186, 1595 |
| BsmBI | CGTCTC | 3 cut(s) | 377, 699, 1186 |
| BsmFI | GGGAC | 4 cut(s) | 31, 493, 1172, 1577 |
| BsmI | GAATGC | 1 cut(s) | 287 |
| Bso31I | GGTCTC | 1 cut(s) | 1046 |
| BsoBI | CYCGRG | 1 cut(s) | 1506 |
| Bsp120I | GGGCCC | 2 cut(s) | 726, 1503 |
| Bsp1286I | GDGCHC | 3 cut(s) | 730, 1023, 1507 |
| Bsp143I | GATC | 4 cut(s) | 59, 159, 499, 1200 |
| Bsp68I | TCGCGA | 1 cut(s) | 282 |
| BspCNI | CTCAG | 5 cut(s) | 496, 673, 1035, 1398, 1576 |
| BspFNI | CGCG | 6 cut(s) | 150, 244, 282, 827, 910, 1131 |
| BspMAI | CTGCAG | 2 cut(s) | 1215, 1630 |
| BspMI | ACCTGC | 1 cut(s) | 240 |
| BspPI | GGATC | 2 cut(s) | 167, 507 |
| BspQI | GCTCTTC | 1 cut(s) | 1011 |
| BspT107I | GGYRCC | 2 cut(s) | 787, 1538 |
| BspTNI | GGTCTC | 1 cut(s) | 1046 |
| BsrBI | CCGCTC | 5 cut(s) | 850, 877, 1090, 1219, 1309 |
| BsrFI | RCCGGY | 1 cut(s) | 1579 |
| BsrI | ACTGG | 6 cut(s) | 14, 214, 400, 436, 617, 1080 |
| BssAI | RCCGGY | 1 cut(s) | 1579 |
| BssECI | CCNNGG | 8 cut(s) | 345, 405, 445, 495, 1004, 1101, 1129, 1506 |
| BssMI | GATC | 4 cut(s) | 59, 159, 499, 1200 |
| BssNI | GRCGYC | 5 cut(s) | 788, 992, 1125, 1539, 1559 |
| BssT1I | CCWWGG | 2 cut(s) | 445, 1101 |
| Bst4CI | ACNGT | 3 cut(s) | 41, 370, 469 |
| Bst6I | CTCTTC | 3 cut(s) | 693, 866, 1011 |
| BstACI | GRCGYC | 5 cut(s) | 788, 992, 1125, 1539, 1559 |
| BstDEI | CTNAG | 7 cut(s) | 483, 681, 1022, 1230, 1320, 1406, 1584 |
| BstDSI | CCRYGG | 1 cut(s) | 1129 |
| BstEII | GGTNACC | 1 cut(s) | 1396 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 449 |
| BstF5I | GGATG | 5 cut(s) | 460, 546, 1149, 1281, 1371 |
| BstFNI | CGCG | 6 cut(s) | 150, 244, 282, 827, 910, 1131 |
| BstH2I | RGCGCY | 5 cut(s) | 516, 659, 673, 791, 1542 |
| BstHHI | GCGC | 7 cut(s) | 515, 658, 672, 790, 829, 912, 1541 |
| BstKTI | GATC | 4 cut(s) | 62, 162, 502, 1203 |
| BstMAI | GTCTC | 9 cut(s) | 377, 444, 640, 690, 699, 1046, 1066, 1186, 1595 |
| BstMBI | GATC | 4 cut(s) | 59, 159, 499, 1200 |
| BstMCI | CGRYCG | 2 cut(s) | 492, 1434 |
| BstNSI | RCATGY | 1 cut(s) | 1525 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1607 |
| BstPI | GGTNACC | 1 cut(s) | 1396 |
| BstSCI | CCNGG | 8 cut(s) | 262, 306, 378, 494, 1004, 1505, 1506, 1640 |
| BstSFI | CTRYAG | 3 cut(s) | 33, 1211, 1626 |
| BstSLI | GKGCMC | 2 cut(s) | 730, 1507 |
| BstUI | CGCG | 6 cut(s) | 150, 244, 282, 827, 910, 1131 |
| BstV1I | GCAGC | 8 cut(s) | 502, 1012, 1197, 1267, 1357, 1414, 1603, 1612 |
| BstV2I | GAAGAC | 2 cut(s) | 770, 1481 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 557 |
| BstZI | CGGCCG | 2 cut(s) | 489, 1431 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1406 |
| BtgI | CCRYGG | 1 cut(s) | 1129 |
| BtgZI | GCGATG | 1 cut(s) | 314 |
| BtrI | CACGTC | 2 cut(s) | 1180, 1573 |
| BtsCI | GGATG | 5 cut(s) | 460, 546, 1149, 1281, 1371 |
| BtsI | GCAGTG | 1 cut(s) | 594 |
| BtsIMutI | CAGTG | 3 cut(s) | 594, 624, 1478 |
| BtuMI | TCGCGA | 1 cut(s) | 282 |
| BveI | ACCTGC | 1 cut(s) | 240 |
| CciNI | GCGGCCGC | 1 cut(s) | 1431 |
| CfoI | GCGC | 7 cut(s) | 515, 658, 672, 790, 829, 912, 1541 |
| Cfr10I | RCCGGY | 1 cut(s) | 1579 |
| Cfr42I | CCGCGG | 1 cut(s) | 1132 |
| Cfr9I | CCCGGG | 1 cut(s) | 1506 |
| CseI | GACGC | 5 cut(s) | 16, 293, 981, 1133, 1548 |
| Csp6I | GTAC | 3 cut(s) | 49, 823, 1422 |
| CviAII | CATG | 3 cut(s) | 337, 974, 1522 |
| CviQI | GTAC | 3 cut(s) | 49, 823, 1422 |
| DdeI | CTNAG | 7 cut(s) | 483, 681, 1022, 1230, 1320, 1406, 1584 |
| DinI | GGCGCC | 2 cut(s) | 789, 1540 |
| DpnI | GATC | 4 cut(s) | 61, 161, 501, 1202 |
| DpnII | GATC | 4 cut(s) | 59, 159, 499, 1200 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 682 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 682 |
| EaeI | YGGCCR | 5 cut(s) | 489, 1146, 1278, 1368, 1431 |
| EagI | CGGCCG | 2 cut(s) | 489, 1431 |
| Eam1104I | CTCTTC | 3 cut(s) | 693, 866, 1011 |
| EarI | CTCTTC | 3 cut(s) | 693, 866, 1011 |
| EciI | GGCGGA | 7 cut(s) | 190, 326, 599, 1094, 1121, 1255, 1345 |
| Ecl136II | GAGCTC | 1 cut(s) | 1021 |
| EclXI | CGGCCG | 2 cut(s) | 489, 1431 |
| Eco130I | CCWWGG | 2 cut(s) | 445, 1101 |
| Eco24I | GRGCYC | 3 cut(s) | 730, 1023, 1507 |
| Eco31I | GGTCTC | 1 cut(s) | 1046 |
| Eco47I | GGWCC | 4 cut(s) | 18, 246, 304, 560 |
| Eco47III | AGCGCT | 1 cut(s) | 514 |
| Eco52I | CGGCCG | 2 cut(s) | 489, 1431 |
| Eco53kI | GAGCTC | 1 cut(s) | 1021 |
| Eco57I | CTGAAG | 2 cut(s) | 922, 1434 |
| Eco81I | CCTNAGG | 1 cut(s) | 1406 |
| Eco88I | CYCGRG | 1 cut(s) | 1506 |
| Eco91I | GGTNACC | 1 cut(s) | 1396 |
| EcoICRI | GAGCTC | 1 cut(s) | 1021 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 449 |
| EcoO109I | RGGNCCY | 3 cut(s) | 18, 726, 1503 |
| EcoO65I | GGTNACC | 1 cut(s) | 1396 |
| EcoT14I | CCWWGG | 2 cut(s) | 445, 1101 |
| EcoT38I | GRGCYC | 3 cut(s) | 730, 1023, 1507 |
| EgeI | GGCGCC | 2 cut(s) | 789, 1540 |
| EheI | GGCGCC | 2 cut(s) | 789, 1540 |
| ErhI | CCWWGG | 2 cut(s) | 445, 1101 |
| Esp3I | CGTCTC | 3 cut(s) | 377, 699, 1186 |
| FaeI | CATG | 3 cut(s) | 340, 977, 1525 |
| FaqI | GGGAC | 4 cut(s) | 31, 493, 1172, 1577 |
| FatI | CATG | 3 cut(s) | 336, 973, 1521 |
| FblI | GTMKAC | 1 cut(s) | 228 |
| FokI | GGATG | 5 cut(s) | 467, 553, 1136, 1288, 1378 |
| FriOI | GRGCYC | 3 cut(s) | 730, 1023, 1507 |
| FspBI | CTAG | 1 cut(s) | 806 |
| GlaI | GCGC | 7 cut(s) | 514, 657, 671, 789, 828, 911, 1540 |
| GsaI | CCCAGC | 7 cut(s) | 364, 645, 884, 1298, 1388, 1503, 1625 |
| GsuI | CTGGAG | 2 cut(s) | 680, 1028 |
| HaeII | RGCGCY | 5 cut(s) | 516, 659, 673, 791, 1542 |
| HgaI | GACGC | 5 cut(s) | 16, 293, 981, 1133, 1548 |
| HhaI | GCGC | 7 cut(s) | 515, 658, 672, 790, 829, 912, 1541 |
| Hin1I | GRCGYC | 5 cut(s) | 788, 992, 1125, 1539, 1559 |
| Hin1II | CATG | 3 cut(s) | 340, 977, 1525 |
| Hin6I | GCGC | 7 cut(s) | 513, 656, 670, 788, 827, 910, 1539 |
| HinP1I | GCGC | 7 cut(s) | 513, 656, 670, 788, 827, 910, 1539 |
| HincII | GTYRAC | 1 cut(s) | 229 |
| HindII | GTYRAC | 1 cut(s) | 229 |
| HinfI | GANTC | 8 cut(s) | 277, 332, 481, 683, 809, 885, 1262, 1352 |
| HphI | GGTGA | 7 cut(s) | 385, 430, 434, 461, 637, 1313, 1403 |
| Hpy166II | GTNNAC | 2 cut(s) | 229, 376 |
| Hpy188I | TCNGA | 6 cut(s) | 97, 406, 486, 504, 738, 1037 |
| Hpy188III | TCNNGA | 4 cut(s) | 281, 292, 689, 1045 |
| Hpy8I | GTNNAC | 2 cut(s) | 229, 376 |
| Hpy99I | CGWCG | 7 cut(s) | 32, 82, 287, 641, 800, 919, 997 |
| HpyCH4III | ACNGT | 3 cut(s) | 41, 370, 469 |
| HpyCH4IV | ACGT | 4 cut(s) | 80, 386, 1179, 1572 |
| HpyCH4V | TGCA | 7 cut(s) | 472, 577, 1213, 1255, 1345, 1553, 1628 |
| HpyF3I | CTNAG | 7 cut(s) | 483, 681, 1022, 1230, 1320, 1406, 1584 |
| HpySE526I | ACGT | 4 cut(s) | 80, 386, 1179, 1572 |
| Hsp92I | GRCGYC | 5 cut(s) | 788, 992, 1125, 1539, 1559 |
| Hsp92II | CATG | 3 cut(s) | 340, 977, 1525 |
| HspAI | GCGC | 7 cut(s) | 513, 656, 670, 788, 827, 910, 1539 |
| KasI | GGCGCC | 2 cut(s) | 787, 1538 |
| KroI | GCCGGC | 1 cut(s) | 1579 |
| KroNI | GCCGGC | 1 cut(s) | 1581 |
| KspI | CCGCGG | 1 cut(s) | 1132 |
| Kzo9I | GATC | 4 cut(s) | 59, 159, 499, 1200 |
| LguI | GCTCTTC | 1 cut(s) | 1011 |
| LmnI | GCTCC | 6 cut(s) | 760, 847, 1165, 1192, 1216, 1306 |
| Lsp1109I | GCAGC | 8 cut(s) | 502, 1012, 1197, 1267, 1357, 1414, 1603, 1612 |
| LweI | GCATC | 3 cut(s) | 411, 417, 531 |
| MaeI | CTAG | 1 cut(s) | 806 |
| MaeII | ACGT | 4 cut(s) | 80, 386, 1179, 1572 |
| MaeIII | GTNAC | 4 cut(s) | 592, 625, 1396, 1560 |
| MalI | GATC | 4 cut(s) | 61, 161, 501, 1202 |
| MbiI | CCGCTC | 5 cut(s) | 850, 877, 1090, 1219, 1309 |
| MboI | GATC | 4 cut(s) | 59, 159, 499, 1200 |
| MboII | GAAGA | 8 cut(s) | 599, 710, 751, 775, 853, 1028, 1040, 1486 |
| MfeI | CAATTG | 1 cut(s) | 921 |
| MhlI | GDGCHC | 3 cut(s) | 730, 1023, 1507 |
| MluCI | AATT | 3 cut(s) | 322, 921, 1373 |
| Mly113I | GGCGCC | 2 cut(s) | 788, 1539 |
| MlyI | GAGTC | 7 cut(s) | 286, 341, 475, 692, 803, 1271, 1361 |
| MmeI | TCCRAC | 3 cut(s) | 127, 1170, 1612 |
| MroNI | GCCGGC | 1 cut(s) | 1579 |
| MseI | TTAA | 3 cut(s) | 90, 239, 1470 |
| MslI | CAYNNNNRTG | 3 cut(s) | 447, 555, 1250 |
| MspA1I | CMGCKG | 5 cut(s) | 1131, 1258, 1348, 1427, 1625 |
| MspR9I | CCNGG | 8 cut(s) | 264, 308, 380, 496, 1006, 1507, 1508, 1642 |
| MunI | CAATTG | 1 cut(s) | 921 |
| Mva1269I | GAATGC | 1 cut(s) | 287 |
| MvnI | CGCG | 6 cut(s) | 150, 244, 282, 827, 910, 1131 |
| NaeI | GCCGGC | 1 cut(s) | 1581 |
| NarI | GGCGCC | 2 cut(s) | 788, 1539 |
| NciI | CCSGG | 8 cut(s) | 264, 308, 380, 496, 1006, 1507, 1508, 1642 |
| NdeII | GATC | 4 cut(s) | 59, 159, 499, 1200 |
| NgoMIV | GCCGGC | 1 cut(s) | 1579 |
| NlaIII | CATG | 3 cut(s) | 340, 977, 1525 |
| NmeAIII | GCCGAG | 3 cut(s) | 370, 844, 923 |
| NmuCI | GTSAC | 3 cut(s) | 592, 625, 1560 |
| NotI | GCGGCCGC | 1 cut(s) | 1431 |
| NruI | TCGCGA | 1 cut(s) | 282 |
| NspI | RCATGY | 1 cut(s) | 1525 |
| OliI | CACNNNNGTG | 2 cut(s) | 447, 555 |
| PciI | ACATGT | 1 cut(s) | 1521 |
| PciSI | GCTCTTC | 1 cut(s) | 1011 |
| PctI | GAATGC | 1 cut(s) | 287 |
| PdiI | GCCGGC | 1 cut(s) | 1581 |
| PfeI | GAWTC | 1 cut(s) | 885 |
| PleI | GAGTC | 7 cut(s) | 285, 340, 475, 691, 803, 1270, 1360 |
| PluTI | GGCGCC | 2 cut(s) | 791, 1542 |
| PpsI | GAGTC | 7 cut(s) | 285, 340, 475, 691, 803, 1270, 1360 |
| PpuMI | RGGWCCY | 1 cut(s) | 18 |
| PscI | ACATGT | 1 cut(s) | 1521 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1607 |
| Psp124BI | GAGCTC | 1 cut(s) | 1023 |
| Psp5II | RGGWCCY | 1 cut(s) | 18 |
| PspEI | GGTNACC | 1 cut(s) | 1396 |
| PspFI | CCCAGC | 7 cut(s) | 360, 641, 880, 1294, 1384, 1499, 1621 |
| PspOMI | GGGCCC | 2 cut(s) | 726, 1503 |
| PspPPI | RGGWCCY | 1 cut(s) | 18 |
| PstI | CTGCAG | 2 cut(s) | 1215, 1630 |
| PvuII | CAGCTG | 1 cut(s) | 1625 |
| RruI | TCGCGA | 1 cut(s) | 282 |
| RsaI | GTAC | 3 cut(s) | 50, 824, 1423 |
| RsaNI | GTAC | 3 cut(s) | 49, 823, 1422 |
| RseI | CAYNNNNRTG | 3 cut(s) | 447, 555, 1250 |
| SacI | GAGCTC | 1 cut(s) | 1023 |
| SacII | CCGCGG | 1 cut(s) | 1132 |
| SalI | GTCGAC | 1 cut(s) | 227 |
| SapI | GCTCTTC | 1 cut(s) | 1011 |
| SaqAI | TTAA | 3 cut(s) | 90, 239, 1470 |
| Sau3AI | GATC | 4 cut(s) | 59, 159, 499, 1200 |
| SchI | GAGTC | 7 cut(s) | 286, 341, 475, 692, 803, 1271, 1361 |
| ScrFI | CCNGG | 8 cut(s) | 264, 308, 380, 496, 1006, 1507, 1508, 1642 |
| SduI | GDGCHC | 3 cut(s) | 730, 1023, 1507 |
| SfaNI | GCATC | 3 cut(s) | 411, 417, 531 |
| SfcI | CTRYAG | 3 cut(s) | 33, 1211, 1626 |
| SfoI | GGCGCC | 2 cut(s) | 789, 1540 |
| Sfr303I | CCGCGG | 1 cut(s) | 1132 |
| SgrBI | CCGCGG | 1 cut(s) | 1132 |
| SinI | GGWCC | 4 cut(s) | 18, 246, 304, 560 |
| SmaI | CCCGGG | 1 cut(s) | 1508 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 447, 555, 1250 |
| SrfI | GCCCGGGC | 1 cut(s) | 1508 |
| Sse9I | AATT | 3 cut(s) | 322, 921, 1373 |
| SspDI | GGCGCC | 2 cut(s) | 787, 1538 |
| SspMI | CTAG | 1 cut(s) | 806 |
| SstI | GAGCTC | 1 cut(s) | 1023 |
| StyD4I | CCNGG | 8 cut(s) | 262, 306, 378, 494, 1004, 1505, 1506, 1640 |
| StyI | CCWWGG | 2 cut(s) | 445, 1101 |
| TaaI | ACNGT | 3 cut(s) | 41, 370, 469 |
| TaiI | ACGT | 4 cut(s) | 83, 389, 1182, 1575 |
| TaqI | TCGA | 8 cut(s) | 62, 112, 228, 275, 291, 761, 995, 1086 |
| TaqII | GACCGA | 1 cut(s) | 1499 |
| TasI | AATT | 3 cut(s) | 322, 921, 1373 |
| TfiI | GAWTC | 1 cut(s) | 885 |
| Tru1I | TTAA | 3 cut(s) | 90, 239, 1470 |
| Tru9I | TTAA | 3 cut(s) | 90, 239, 1470 |
| TscAI | CASTG | 3 cut(s) | 601, 624, 1485 |
| TseFI | GTSAC | 3 cut(s) | 592, 625, 1560 |
| TseI | GCWGC | 8 cut(s) | 515, 1000, 1210, 1255, 1345, 1427, 1616, 1625 |
| Tsp45I | GTSAC | 3 cut(s) | 592, 625, 1560 |
| TspDTI | ATGAA | 2 cut(s) | 1188, 1301 |
| TspMI | CCCGGG | 1 cut(s) | 1506 |
| TspRI | CASTG | 3 cut(s) | 601, 624, 1485 |
| VpaK11BI | GGWCC | 4 cut(s) | 18, 246, 304, 560 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 449 |
| XceI | RCATGY | 1 cut(s) | 1525 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 443 |
| XmaI | CCCGGG | 1 cut(s) | 1506 |
| XmiI | GTMKAC | 1 cut(s) | 228 |
| XspI | CTAG | 1 cut(s) | 806 |