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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 1883 |
| AccB1I | GGYRCC | 4 cut(s) | 1222, 1793, 1883, 2006 |
| AccBSI | CCGCTC | 3 cut(s) | 1087, 1435, 1564 |
| AccI | GTMKAC | 2 cut(s) | 854, 1610 |
| AccIII | TCCGGA | 1 cut(s) | 220 |
| AclWI | GGATC | 1 cut(s) | 899 |
| AcoI | YGGCCR | 3 cut(s) | 568, 1443, 1836 |
| AcsI | RAATTY | 1 cut(s) | 1721 |
| AcuI | CTGAAG | 5 cut(s) | 753, 1357, 1689, 1802, 2100 |
| AcvI | CACGTG | 1 cut(s) | 1970 |
| AcyI | GRCGYC | 4 cut(s) | 163, 621, 1223, 1794 |
| AfaI | GTAC | 3 cut(s) | 595, 1678, 1885 |
| AfeI | AGCGCT | 1 cut(s) | 1063 |
| AflIII | ACRYGT | 3 cut(s) | 1488, 1522, 1969 |
| AgsI | TTSAA | 1 cut(s) | 1708 |
| AjiI | CACGTC | 1 cut(s) | 530 |
| AjnI | CCWGG | 4 cut(s) | 181, 565, 1093, 1100 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1280, 1312 |
| Alw26I | GTCTC | 6 cut(s) | 430, 491, 769, 1531, 1850, 2009 |
| AlwI | GGATC | 1 cut(s) | 899 |
| Ama87I | CYCGRG | 2 cut(s) | 290, 1995 |
| Aor13HI | TCCGGA | 1 cut(s) | 220 |
| Aor51HI | AGCGCT | 1 cut(s) | 1063 |
| ApaI | GGGCCC | 1 cut(s) | 1762 |
| ApoI | RAATTY | 1 cut(s) | 1721 |
| Asp718I | GGTACC | 1 cut(s) | 1883 |
| AspS9I | GGNCC | 8 cut(s) | 157, 1097, 1109, 1180, 1758, 1759, 1929, 2040 |
| AsuC2I | CCSGG | 7 cut(s) | 204, 636, 770, 1466, 1756, 1996, 1997 |
| AsuHPI | GGTGA | 8 cut(s) | 41, 290, 532, 800, 820, 1183, 1292, 1658 |
| AsuII | TTCGAA | 1 cut(s) | 1196 |
| AvaI | CYCGRG | 2 cut(s) | 290, 1995 |
| AvaII | GGWCC | 3 cut(s) | 157, 1929, 2040 |
| AxyI | CCTNAGG | 1 cut(s) | 1661 |
| BaeGI | GKGCMC | 1 cut(s) | 1762 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 799, 832 |
| BalI | TGGCCA | 1 cut(s) | 1445 |
| BanI | GGYRCC | 4 cut(s) | 1222, 1793, 1883, 2006 |
| BanII | GRGCYC | 1 cut(s) | 1762 |
| BbrPI | CACGTG | 1 cut(s) | 1970 |
| BbsI | GAAGAC | 1 cut(s) | 566 |
| BccI | CCATC | 9 cut(s) | 118, 218, 819, 1001, 1002, 1271, 1308, 1502, 1805 |
| BceAI | ACGGC | 4 cut(s) | 555, 1214, 1368, 1811 |
| BciT130I | CCWGG | 4 cut(s) | 183, 567, 1095, 1102 |
| BciVI | GTATCC | 1 cut(s) | 1856 |
| BcnI | CCSGG | 7 cut(s) | 204, 636, 770, 1466, 1756, 1996, 1997 |
| BcoDI | GTCTC | 6 cut(s) | 430, 491, 769, 1531, 1850, 2009 |
| BfmI | CTRYAG | 2 cut(s) | 1065, 1556 |
| BfoI | RGCGCY | 4 cut(s) | 1065, 1226, 1692, 1797 |
| BfuI | GTATCC | 1 cut(s) | 1856 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 115, 183 |
| BlpI | GCTNAGC | 1 cut(s) | 490 |
| BmcAI | AGTACT | 1 cut(s) | 595 |
| Bme18I | GGWCC | 3 cut(s) | 157, 1929, 2040 |
| BmeT110I | CYCGRG | 2 cut(s) | 290, 1995 |
| BmgBI | CACGTC | 1 cut(s) | 530 |
| BmgT120I | GGNCC | 8 cut(s) | 157, 1097, 1109, 1180, 1758, 1759, 1929, 2040 |
| BmrI | ACTGGG | 1 cut(s) | 82 |
| BmsI | GCATC | 3 cut(s) | 96, 1080, 1823 |
| BmuI | ACTGGG | 1 cut(s) | 82 |
| BoxI | GACNNNNGTC | 1 cut(s) | 126 |
| BpiI | GAAGAC | 1 cut(s) | 566 |
| BpmI | CTGGAG | 4 cut(s) | 170, 1307, 1661, 1899 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 490 |
| Bpu14I | TTCGAA | 1 cut(s) | 1196 |
| BpuEI | CTTGAG | 1 cut(s) | 1020 |
| BpuMI | CCSGG | 7 cut(s) | 204, 636, 770, 1466, 1756, 1996, 1997 |
| Bsa29I | ATCGAT | 1 cut(s) | 2060 |
| BsaAI | YACGTR | 1 cut(s) | 1970 |
| BsaHI | GRCGYC | 4 cut(s) | 163, 621, 1223, 1794 |
| BsaI | GGTCTC | 1 cut(s) | 2009 |
| BsaWI | WCCGGW | 2 cut(s) | 220, 964 |
| Bse118I | RCCGGY | 5 cut(s) | 1015, 1043, 1132, 1168, 1834 |
| Bse1I | ACTGG | 3 cut(s) | 88, 946, 988 |
| Bse21I | CCTNAGG | 1 cut(s) | 1661 |
| BseAI | TCCGGA | 1 cut(s) | 220 |
| BseBI | CCWGG | 4 cut(s) | 183, 567, 1095, 1102 |
| BseCI | ATCGAT | 1 cut(s) | 2060 |
| BseGI | GGATG | 8 cut(s) | 229, 399, 460, 692, 1012, 1095, 1814, 1915 |
| BseMII | CTCAG | 5 cut(s) | 443, 504, 1652, 1731, 1905 |
| BseNI | ACTGG | 3 cut(s) | 88, 946, 988 |
| BseRI | GAGGAG | 2 cut(s) | 19, 26 |
| BseSI | GKGCMC | 1 cut(s) | 1762 |
| BseX3I | CGGCCG | 1 cut(s) | 1836 |
| BseYI | CCCAGC | 6 cut(s) | 322, 659, 1070, 1818, 1932, 2021 |
| BsgI | GTGCAG | 1 cut(s) | 1619 |
| Bsh1285I | CGRYCG | 3 cut(s) | 269, 1839, 2060 |
| BshNI | GGYRCC | 4 cut(s) | 1222, 1793, 1883, 2006 |
| BshVI | ATCGAT | 1 cut(s) | 2060 |
| BsiEI | CGRYCG | 3 cut(s) | 269, 1839, 2060 |
| BsiHKCI | CYCGRG | 2 cut(s) | 290, 1995 |
| BslFI | GGGAC | 6 cut(s) | 81, 177, 541, 976, 1517, 1915 |
| BsmAI | GTCTC | 6 cut(s) | 430, 491, 769, 1531, 1850, 2009 |
| BsmBI | CGTCTC | 2 cut(s) | 769, 1531 |
| BsmFI | GGGAC | 6 cut(s) | 81, 177, 541, 976, 1517, 1915 |
| Bso31I | GGTCTC | 1 cut(s) | 2009 |
| BsoBI | CYCGRG | 2 cut(s) | 290, 1995 |
| Bsp119I | TTCGAA | 1 cut(s) | 1196 |
| Bsp120I | GGGCCC | 1 cut(s) | 1758 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1762 |
| Bsp13I | TCCGGA | 1 cut(s) | 220 |
| Bsp143I | GATC | 6 cut(s) | 43, 145, 891, 1545, 2044, 2057 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 490 |
| BspCNI | CTCAG | 5 cut(s) | 442, 503, 1653, 1732, 1904 |
| BspDI | ATCGAT | 1 cut(s) | 2060 |
| BspEI | TCCGGA | 1 cut(s) | 220 |
| BspHI | TCATGA | 1 cut(s) | 1269 |
| BspMAI | CTGCAG | 2 cut(s) | 1069, 1560 |
| BspPI | GGATC | 1 cut(s) | 899 |
| BspT104I | TTCGAA | 1 cut(s) | 1196 |
| BspT107I | GGYRCC | 4 cut(s) | 1222, 1793, 1883, 2006 |
| BspTNI | GGTCTC | 1 cut(s) | 2009 |
| BsrBI | CCGCTC | 3 cut(s) | 1087, 1435, 1564 |
| BsrFI | RCCGGY | 5 cut(s) | 1015, 1043, 1132, 1168, 1834 |
| BsrI | ACTGG | 3 cut(s) | 88, 946, 988 |
| BssAI | RCCGGY | 5 cut(s) | 1015, 1043, 1132, 1168, 1834 |
| BssMI | GATC | 6 cut(s) | 43, 145, 891, 1545, 2044, 2057 |
| BssNAI | GTATAC | 1 cut(s) | 855 |
| BssNI | GRCGYC | 4 cut(s) | 163, 621, 1223, 1794 |
| BssT1I | CCWWGG | 2 cut(s) | 1446, 1575 |
| Bst1107I | GTATAC | 1 cut(s) | 855 |
| Bst2UI | CCWGG | 4 cut(s) | 183, 567, 1095, 1102 |
| Bst4CI | ACNGT | 9 cut(s) | 243, 251, 302, 593, 700, 765, 1150, 1204, 1314 |
| Bst6I | CTCTTC | 3 cut(s) | 30, 867, 1665 |
| BstACI | GRCGYC | 4 cut(s) | 163, 621, 1223, 1794 |
| BstBAI | YACGTR | 1 cut(s) | 1970 |
| BstBI | TTCGAA | 1 cut(s) | 1196 |
| BstDEI | CTNAG | 7 cut(s) | 52, 429, 490, 795, 1661, 1740, 1891 |
| BstDSI | CCRYGG | 3 cut(s) | 370, 1350, 1680 |
| BstEII | GGTNACC | 1 cut(s) | 1651 |
| BstF5I | GGATG | 8 cut(s) | 229, 399, 460, 692, 1012, 1095, 1814, 1915 |
| BstH2I | RGCGCY | 4 cut(s) | 1065, 1226, 1692, 1797 |
| BstKTI | GATC | 6 cut(s) | 46, 148, 894, 1548, 2047, 2060 |
| BstMAI | GTCTC | 6 cut(s) | 430, 491, 769, 1531, 1850, 2009 |
| BstMBI | GATC | 6 cut(s) | 43, 145, 891, 1545, 2044, 2057 |
| BstMCI | CGRYCG | 3 cut(s) | 269, 1839, 2060 |
| BstNI | CCWGG | 4 cut(s) | 183, 567, 1095, 1102 |
| BstNSI | RCATGY | 1 cut(s) | 78 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 126 |
| BstPI | GGTNACC | 1 cut(s) | 1651 |
| BstSFI | CTRYAG | 2 cut(s) | 1065, 1556 |
| BstSLI | GKGCMC | 1 cut(s) | 1762 |
| BstV2I | GAAGAC | 1 cut(s) | 566 |
| BstZ17I | GTATAC | 1 cut(s) | 855 |
| BstZI | CGGCCG | 1 cut(s) | 1836 |
| Bsu15I | ATCGAT | 1 cut(s) | 2060 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1661 |
| BsuI | GTATCC | 1 cut(s) | 1856 |
| BsuTUI | ATCGAT | 1 cut(s) | 2060 |
| BtgI | CCRYGG | 3 cut(s) | 370, 1350, 1680 |
| BtgZI | GCGATG | 1 cut(s) | 1748 |
| BtrI | CACGTC | 1 cut(s) | 530 |
| BtsCI | GGATG | 8 cut(s) | 229, 399, 460, 692, 1012, 1095, 1814, 1915 |
| BtsIMutI | CAGTG | 2 cut(s) | 282, 1146 |
| CciI | TCATGA | 1 cut(s) | 1269 |
| Cfr10I | RCCGGY | 5 cut(s) | 1015, 1043, 1132, 1168, 1834 |
| Cfr13I | GGNCC | 8 cut(s) | 157, 1097, 1109, 1180, 1758, 1759, 1929, 2040 |
| Cfr42I | CCGCGG | 2 cut(s) | 373, 1683 |
| Cfr9I | CCCGGG | 1 cut(s) | 1995 |
| ClaI | ATCGAT | 1 cut(s) | 2060 |
| CseI | GACGC | 7 cut(s) | 116, 124, 131, 171, 331, 629, 1513 |
| Csp6I | GTAC | 3 cut(s) | 594, 1677, 1884 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 820, 855, 1339, 1374 |
| CviAII | CATG | 2 cut(s) | 75, 1270 |
| CviQI | GTAC | 3 cut(s) | 594, 1677, 1884 |
| DdeI | CTNAG | 7 cut(s) | 52, 429, 490, 795, 1661, 1740, 1891 |
| DinI | GGCGCC | 2 cut(s) | 1224, 1795 |
| DpnI | GATC | 6 cut(s) | 45, 147, 893, 1547, 2046, 2059 |
| DpnII | GATC | 6 cut(s) | 43, 145, 891, 1545, 2044, 2057 |
| EaeI | YGGCCR | 3 cut(s) | 568, 1443, 1836 |
| EagI | CGGCCG | 1 cut(s) | 1836 |
| Eam1104I | CTCTTC | 3 cut(s) | 30, 867, 1665 |
| EarI | CTCTTC | 3 cut(s) | 30, 867, 1665 |
| EciI | GGCGGA | 7 cut(s) | 323, 922, 1151, 1418, 1439, 1466, 1733 |
| EclXI | CGGCCG | 1 cut(s) | 1836 |
| Eco130I | CCWWGG | 2 cut(s) | 1446, 1575 |
| Eco24I | GRGCYC | 1 cut(s) | 1762 |
| Eco31I | GGTCTC | 1 cut(s) | 2009 |
| Eco32I | GATATC | 1 cut(s) | 313 |
| Eco47I | GGWCC | 3 cut(s) | 157, 1929, 2040 |
| Eco47III | AGCGCT | 1 cut(s) | 1063 |
| Eco52I | CGGCCG | 1 cut(s) | 1836 |
| Eco57I | CTGAAG | 5 cut(s) | 753, 1357, 1689, 1802, 2100 |
| Eco72I | CACGTG | 1 cut(s) | 1970 |
| Eco81I | CCTNAGG | 1 cut(s) | 1661 |
| Eco88I | CYCGRG | 2 cut(s) | 290, 1995 |
| Eco91I | GGTNACC | 1 cut(s) | 1651 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1758, 1929 |
| EcoO65I | GGTNACC | 1 cut(s) | 1651 |
| EcoRII | CCWGG | 4 cut(s) | 181, 565, 1093, 1100 |
| EcoRV | GATATC | 1 cut(s) | 313 |
| EcoT14I | CCWWGG | 2 cut(s) | 1446, 1575 |
| EcoT38I | GRGCYC | 1 cut(s) | 1762 |
| EgeI | GGCGCC | 2 cut(s) | 1224, 1795 |
| EheI | GGCGCC | 2 cut(s) | 1224, 1795 |
| ErhI | CCWWGG | 2 cut(s) | 1446, 1575 |
| Esp3I | CGTCTC | 2 cut(s) | 769, 1531 |
| FaeI | CATG | 2 cut(s) | 78, 1273 |
| FaqI | GGGAC | 6 cut(s) | 81, 177, 541, 976, 1517, 1915 |
| FatI | CATG | 2 cut(s) | 74, 1269 |
| FblI | GTMKAC | 2 cut(s) | 854, 1610 |
| FokI | GGATG | 8 cut(s) | 236, 406, 467, 679, 1019, 1102, 1801, 1902 |
| FriOI | GRGCYC | 1 cut(s) | 1762 |
| GsaI | CCCAGC | 6 cut(s) | 326, 663, 1074, 1822, 1936, 2025 |
| GsuI | CTGGAG | 4 cut(s) | 170, 1307, 1661, 1899 |
| HaeII | RGCGCY | 4 cut(s) | 1065, 1226, 1692, 1797 |
| HgaI | GACGC | 7 cut(s) | 116, 124, 131, 171, 331, 629, 1513 |
| Hin1I | GRCGYC | 4 cut(s) | 163, 621, 1223, 1794 |
| Hin1II | CATG | 2 cut(s) | 78, 1273 |
| HindIII | AAGCTT | 2 cut(s) | 98, 1586 |
| HinfI | GANTC | 8 cut(s) | 190, 295, 667, 1033, 1244, 1607, 1939, 2053 |
| HphI | GGTGA | 8 cut(s) | 41, 290, 532, 800, 820, 1183, 1292, 1658 |
| Hpy166II | GTNNAC | 3 cut(s) | 808, 855, 1611 |
| Hpy188I | TCNGA | 9 cut(s) | 96, 105, 161, 896, 932, 939, 958, 1960, 2044 |
| Hpy188III | TCNNGA | 4 cut(s) | 149, 221, 292, 1270 |
| Hpy8I | GTNNAC | 3 cut(s) | 808, 855, 1611 |
| Hpy99I | CGWCG | 6 cut(s) | 125, 132, 140, 165, 623, 783 |
| HpyCH4III | ACNGT | 9 cut(s) | 243, 251, 302, 593, 700, 765, 1150, 1204, 1314 |
| HpyCH4IV | ACGT | 4 cut(s) | 529, 1470, 1488, 1969 |
| HpyCH4V | TGCA | 6 cut(s) | 842, 1024, 1067, 1129, 1558, 1600 |
| HpyF3I | CTNAG | 7 cut(s) | 52, 429, 490, 795, 1661, 1740, 1891 |
| HpySE526I | ACGT | 4 cut(s) | 529, 1470, 1488, 1969 |
| Hsp92I | GRCGYC | 4 cut(s) | 163, 621, 1223, 1794 |
| Hsp92II | CATG | 2 cut(s) | 78, 1273 |
| KasI | GGCGCC | 2 cut(s) | 1222, 1793 |
| KflI | GGGWCCC | 1 cut(s) | 1929 |
| Kpn2I | TCCGGA | 1 cut(s) | 220 |
| KpnI | GGTACC | 1 cut(s) | 1887 |
| KroI | GCCGGC | 2 cut(s) | 1043, 1834 |
| KroNI | GCCGGC | 2 cut(s) | 1045, 1836 |
| KspI | CCGCGG | 2 cut(s) | 373, 1683 |
| Kzo9I | GATC | 6 cut(s) | 43, 145, 891, 1545, 2044, 2057 |
| LmnI | GCTCC | 6 cut(s) | 372, 750, 1084, 1195, 1537, 1561 |
| LweI | GCATC | 3 cut(s) | 96, 1080, 1823 |
| MaeII | ACGT | 4 cut(s) | 529, 1470, 1488, 1969 |
| MalI | GATC | 6 cut(s) | 45, 147, 893, 1547, 2046, 2059 |
| MbiI | CCGCTC | 3 cut(s) | 1087, 1435, 1564 |
| MboI | GATC | 6 cut(s) | 43, 145, 891, 1545, 2044, 2057 |
| MfeI | CAATTG | 1 cut(s) | 1356 |
| MhlI | GDGCHC | 1 cut(s) | 1762 |
| MlsI | TGGCCA | 1 cut(s) | 1445 |
| MluCI | AATT | 3 cut(s) | 234, 1356, 1721 |
| MluI | ACGCGT | 1 cut(s) | 1522 |
| MluNI | TGGCCA | 1 cut(s) | 1445 |
| Mly113I | GGCGCC | 2 cut(s) | 1223, 1794 |
| MlyI | GAGTC | 5 cut(s) | 199, 1027, 1616, 1933, 2062 |
| MmeI | TCCRAC | 4 cut(s) | 184, 955, 1515, 1954 |
| Mox20I | TGGCCA | 1 cut(s) | 1445 |
| MroI | TCCGGA | 1 cut(s) | 220 |
| MroNI | GCCGGC | 2 cut(s) | 1043, 1834 |
| MscI | TGGCCA | 1 cut(s) | 1445 |
| MseI | TTAA | 2 cut(s) | 630, 1725 |
| MslI | CAYNNNNRTG | 2 cut(s) | 999, 1775 |
| Msp20I | TGGCCA | 1 cut(s) | 1445 |
| MunI | CAATTG | 1 cut(s) | 1356 |
| MvaI | CCWGG | 4 cut(s) | 183, 567, 1095, 1102 |
| NaeI | GCCGGC | 2 cut(s) | 1045, 1836 |
| NarI | GGCGCC | 2 cut(s) | 1223, 1794 |
| NciI | CCSGG | 7 cut(s) | 204, 636, 770, 1466, 1756, 1996, 1997 |
| NdeII | GATC | 6 cut(s) | 43, 145, 891, 1545, 2044, 2057 |
| NgoMIV | GCCGGC | 2 cut(s) | 1043, 1834 |
| NlaIII | CATG | 2 cut(s) | 78, 1273 |
| NmeAIII | GCCGAG | 4 cut(s) | 191, 1279, 1358, 1864 |
| NmuCI | GTSAC | 7 cut(s) | 382, 443, 504, 525, 1144, 1457, 1965 |
| NspI | RCATGY | 1 cut(s) | 78 |
| NspV | TTCGAA | 1 cut(s) | 1196 |
| PagI | TCATGA | 1 cut(s) | 1269 |
| PdiI | GCCGGC | 2 cut(s) | 1045, 1836 |
| PfeI | GAWTC | 3 cut(s) | 295, 667, 1244 |
| PflFI | GACNNNGTC | 2 cut(s) | 441, 502 |
| PfoI | TCCNGGA | 1 cut(s) | 768 |
| Ple19I | CGATCG | 1 cut(s) | 2060 |
| PleI | GAGTC | 5 cut(s) | 198, 1027, 1615, 1933, 2061 |
| PluTI | GGCGCC | 2 cut(s) | 1226, 1797 |
| PmaCI | CACGTG | 1 cut(s) | 1970 |
| PmlI | CACGTG | 1 cut(s) | 1970 |
| PpsI | GAGTC | 5 cut(s) | 198, 1027, 1615, 1933, 2061 |
| Ppu21I | YACGTR | 1 cut(s) | 1970 |
| PpuMI | RGGWCCY | 1 cut(s) | 1929 |
| PshAI | GACNNNNGTC | 1 cut(s) | 126 |
| Psp5II | RGGWCCY | 1 cut(s) | 1929 |
| Psp6I | CCWGG | 4 cut(s) | 181, 565, 1093, 1100 |
| PspCI | CACGTG | 1 cut(s) | 1970 |
| PspEI | GGTNACC | 1 cut(s) | 1651 |
| PspFI | CCCAGC | 6 cut(s) | 322, 659, 1070, 1818, 1932, 2021 |
| PspGI | CCWGG | 4 cut(s) | 181, 565, 1093, 1100 |
| PspOMI | GGGCCC | 1 cut(s) | 1758 |
| PspPI | GGNCC | 8 cut(s) | 157, 1097, 1109, 1180, 1758, 1759, 1929, 2040 |
| PspPPI | RGGWCCY | 1 cut(s) | 1929 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1250, 1282 |
| PstI | CTGCAG | 2 cut(s) | 1069, 1560 |
| PsyI | GACNNNGTC | 2 cut(s) | 441, 502 |
| PvuI | CGATCG | 1 cut(s) | 2060 |
| PvuII | CAGCTG | 1 cut(s) | 1070 |
| RsaI | GTAC | 3 cut(s) | 595, 1678, 1885 |
| RsaNI | GTAC | 3 cut(s) | 594, 1677, 1884 |
| RseI | CAYNNNNRTG | 2 cut(s) | 999, 1775 |
| SacII | CCGCGG | 2 cut(s) | 373, 1683 |
| SaqAI | TTAA | 2 cut(s) | 630, 1725 |
| Sau3AI | GATC | 6 cut(s) | 43, 145, 891, 1545, 2044, 2057 |
| Sau96I | GGNCC | 8 cut(s) | 157, 1097, 1109, 1180, 1758, 1759, 1929, 2040 |
| ScaI | AGTACT | 1 cut(s) | 595 |
| SchI | GAGTC | 5 cut(s) | 199, 1027, 1616, 1933, 2062 |
| SduI | GDGCHC | 1 cut(s) | 1762 |
| SfaNI | GCATC | 3 cut(s) | 96, 1080, 1823 |
| SfcI | CTRYAG | 2 cut(s) | 1065, 1556 |
| SfoI | GGCGCC | 2 cut(s) | 1224, 1795 |
| Sfr303I | CCGCGG | 2 cut(s) | 373, 1683 |
| SfuI | TTCGAA | 1 cut(s) | 1196 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1168 |
| SgrBI | CCGCGG | 2 cut(s) | 373, 1683 |
| SinI | GGWCC | 3 cut(s) | 157, 1929, 2040 |
| SmaI | CCCGGG | 1 cut(s) | 1997 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 999, 1775 |
| SmlI | CTYRAG | 1 cut(s) | 1035 |
| SmoI | CTYRAG | 1 cut(s) | 1035 |
| Sse9I | AATT | 3 cut(s) | 234, 1356, 1721 |
| SspDI | GGCGCC | 2 cut(s) | 1222, 1793 |
| StyI | CCWWGG | 2 cut(s) | 1446, 1575 |
| TaaI | ACNGT | 9 cut(s) | 243, 251, 302, 593, 700, 765, 1150, 1204, 1314 |
| TaiI | ACGT | 4 cut(s) | 532, 1473, 1491, 1972 |
| TaqI | TCGA | 7 cut(s) | 60, 670, 1196, 1800, 2056, 2060, 2094 |
| TaqII | GACCGA | 1 cut(s) | 1640 |
| TasI | AATT | 3 cut(s) | 234, 1356, 1721 |
| TatI | WGTACW | 1 cut(s) | 593 |
| TfiI | GAWTC | 3 cut(s) | 295, 667, 1244 |
| Tru1I | TTAA | 2 cut(s) | 630, 1725 |
| Tru9I | TTAA | 2 cut(s) | 630, 1725 |
| TscAI | CASTG | 2 cut(s) | 282, 1153 |
| TseFI | GTSAC | 7 cut(s) | 382, 443, 504, 525, 1144, 1457, 1965 |
| Tsp45I | GTSAC | 7 cut(s) | 382, 443, 504, 525, 1144, 1457, 1965 |
| TspDTI | ATGAA | 2 cut(s) | 1258, 1533 |
| TspGWI | ACGGA | 1 cut(s) | 335 |
| TspMI | CCCGGG | 1 cut(s) | 1995 |
| TspRI | CASTG | 2 cut(s) | 282, 1153 |
| Tth111I | GACNNNGTC | 2 cut(s) | 441, 502 |
| VpaK11BI | GGWCC | 3 cut(s) | 157, 1929, 2040 |
| XapI | RAATTY | 1 cut(s) | 1721 |
| XceI | RCATGY | 1 cut(s) | 78 |
| XmaI | CCCGGG | 1 cut(s) | 1995 |
| XmiI | GTMKAC | 2 cut(s) | 854, 1610 |
| ZrmI | AGTACT | 1 cut(s) | 595 |