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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 763 |
| AatII | GACGTC | 1 cut(s) | 1215 |
| Acc36I | ACCTGC | 1 cut(s) | 1616 |
| AccB1I | GGYRCC | 1 cut(s) | 1535 |
| AccBSI | CCGCTC | 6 cut(s) | 278, 619, 937, 1177, 1255, 1306 |
| AccI | GTMKAC | 2 cut(s) | 290, 1515 |
| AccII | CGCG | 8 cut(s) | 301, 303, 318, 363, 599, 776, 997, 1266 |
| AclWI | GGATC | 1 cut(s) | 588 |
| AcoI | YGGCCR | 5 cut(s) | 570, 1185, 1233, 1365, 1428 |
| AcuI | CTGAAG | 2 cut(s) | 1009, 1431 |
| AcvI | CACGTG | 1 cut(s) | 1715 |
| AcyI | GRCGYC | 4 cut(s) | 1079, 1212, 1536, 1556 |
| AfaI | GTAC | 4 cut(s) | 212, 1420, 1572, 1630 |
| AflIII | ACRYGT | 2 cut(s) | 1264, 1518 |
| AgsI | TTSAA | 5 cut(s) | 374, 659, 1442, 1450, 1525 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 163 |
| AleI | CACNNNNGTG | 1 cut(s) | 636 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 926, 958 |
| AluBI | AGCT | 6 cut(s) | 139, 892, 1108, 1225, 1409, 1622 |
| AluI | AGCT | 6 cut(s) | 139, 892, 1108, 1225, 1409, 1622 |
| Alw21I | GWGCWC | 1 cut(s) | 1110 |
| Alw26I | GTCTC | 7 cut(s) | 458, 525, 771, 1133, 1153, 1273, 1592 |
| AlwI | GGATC | 1 cut(s) | 588 |
| Ama87I | CYCGRG | 5 cut(s) | 461, 602, 1503, 1636, 1740 |
| ApaI | GGGCCC | 2 cut(s) | 811, 1504 |
| ApeKI | GCWGC | 8 cut(s) | 93, 836, 1087, 1297, 1342, 1424, 1622, 1641 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 54 |
| AsuHPI | GGTGA | 4 cut(s) | 466, 542, 718, 1400 |
| AsuII | TTCGAA | 1 cut(s) | 842 |
| AvaI | CYCGRG | 5 cut(s) | 461, 602, 1503, 1636, 1740 |
| AvaII | GGWCC | 6 cut(s) | 180, 385, 561, 641, 714, 1674 |
| BaeGI | GKGCMC | 2 cut(s) | 811, 1504 |
| BalI | TGGCCA | 2 cut(s) | 1187, 1235 |
| BanI | GGYRCC | 1 cut(s) | 1535 |
| BanII | GRGCYC | 3 cut(s) | 811, 1110, 1504 |
| BbrPI | CACGTG | 1 cut(s) | 1715 |
| BbsI | GAAGAC | 2 cut(s) | 851, 1478 |
| Bbv12I | GWGCWC | 1 cut(s) | 1110 |
| BbvI | GCAGC | 8 cut(s) | 80, 823, 1099, 1284, 1354, 1411, 1609, 1653 |
| BccI | CCATC | 4 cut(s) | 530, 960, 1244, 1357 |
| BceAI | ACGGC | 4 cut(s) | 585, 774, 798, 1020 |
| BcoDI | GTCTC | 7 cut(s) | 458, 525, 771, 1133, 1153, 1273, 1592 |
| BfaI | CTAG | 1 cut(s) | 893 |
| BfmI | CTRYAG | 3 cut(s) | 195, 1298, 1623 |
| BfoI | RGCGCY | 7 cut(s) | 88, 134, 476, 597, 754, 1317, 1539 |
| BfuAI | ACCTGC | 1 cut(s) | 1616 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 833 |
| BlpI | GCTNAGC | 1 cut(s) | 476 |
| Bme18I | GGWCC | 6 cut(s) | 180, 385, 561, 641, 714, 1674 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 163 |
| BmeT110I | CYCGRG | 5 cut(s) | 461, 602, 1503, 1636, 1740 |
| BmsI | GCATC | 4 cut(s) | 492, 498, 612, 862 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1604 |
| BpiI | GAAGAC | 2 cut(s) | 851, 1478 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 881, 913 |
| BpmI | CTGGAG | 3 cut(s) | 761, 1115, 1748 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1109, 1317 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 476 |
| Bpu14I | TTCGAA | 1 cut(s) | 842 |
| BpuEI | CTTGAG | 1 cut(s) | 133 |
| BsaAI | YACGTR | 1 cut(s) | 1715 |
| BsaHI | GRCGYC | 4 cut(s) | 1079, 1212, 1536, 1556 |
| BsaI | GGTCTC | 1 cut(s) | 1133 |
| BsaWI | WCCGGW | 1 cut(s) | 493 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 404, 434, 777, 807, 926, 956 |
| Bse118I | RCCGGY | 3 cut(s) | 120, 572, 1576 |
| Bse1I | ACTGG | 5 cut(s) | 176, 481, 517, 698, 1167 |
| BseGI | GGATG | 4 cut(s) | 541, 627, 1368, 1660 |
| BseMII | CTCAG | 7 cut(s) | 143, 249, 659, 753, 1123, 1394, 1572 |
| BseNI | ACTGG | 5 cut(s) | 176, 481, 517, 698, 1167 |
| BseSI | GKGCMC | 2 cut(s) | 811, 1504 |
| BseX3I | CGGCCG | 2 cut(s) | 570, 1428 |
| BseXI | GCAGC | 8 cut(s) | 80, 823, 1099, 1284, 1354, 1411, 1609, 1653 |
| BseYI | CCCAGC | 7 cut(s) | 441, 722, 967, 1381, 1496, 1618, 1677 |
| BsgI | GTGCAG | 2 cut(s) | 572, 1361 |
| Bsh1236I | CGCG | 8 cut(s) | 301, 303, 318, 363, 599, 776, 997, 1266 |
| Bsh1285I | CGRYCG | 3 cut(s) | 242, 573, 1431 |
| BshNI | GGYRCC | 1 cut(s) | 1535 |
| BsiEI | CGRYCG | 3 cut(s) | 242, 573, 1431 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1110 |
| BsiHKCI | CYCGRG | 5 cut(s) | 461, 602, 1503, 1636, 1740 |
| BslFI | GGGAC | 8 cut(s) | 170, 193, 294, 574, 1068, 1199, 1574, 1660 |
| BsmAI | GTCTC | 7 cut(s) | 458, 525, 771, 1133, 1153, 1273, 1592 |
| BsmBI | CGTCTC | 2 cut(s) | 458, 1273 |
| BsmFI | GGGAC | 8 cut(s) | 170, 193, 294, 574, 1068, 1199, 1574, 1660 |
| Bso31I | GGTCTC | 1 cut(s) | 1133 |
| BsoBI | CYCGRG | 5 cut(s) | 461, 602, 1503, 1636, 1740 |
| Bsp119I | TTCGAA | 1 cut(s) | 842 |
| Bsp120I | GGGCCC | 2 cut(s) | 807, 1500 |
| Bsp1286I | GDGCHC | 3 cut(s) | 811, 1110, 1504 |
| Bsp143I | GATC | 4 cut(s) | 141, 221, 580, 1287 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 476 |
| Bsp19I | CCATGG | 1 cut(s) | 381 |
| Bsp68I | TCGCGA | 1 cut(s) | 363 |
| BspCNI | CTCAG | 7 cut(s) | 144, 250, 658, 754, 1122, 1395, 1573 |
| BspFNI | CGCG | 8 cut(s) | 301, 303, 318, 363, 599, 776, 997, 1266 |
| BspMAI | CTGCAG | 2 cut(s) | 1302, 1627 |
| BspMI | ACCTGC | 1 cut(s) | 1616 |
| BspPI | GGATC | 1 cut(s) | 588 |
| BspT104I | TTCGAA | 1 cut(s) | 842 |
| BspT107I | GGYRCC | 1 cut(s) | 1535 |
| BspTNI | GGTCTC | 1 cut(s) | 1133 |
| BsrBI | CCGCTC | 6 cut(s) | 278, 619, 937, 1177, 1255, 1306 |
| BsrFI | RCCGGY | 3 cut(s) | 120, 572, 1576 |
| BsrI | ACTGG | 5 cut(s) | 176, 481, 517, 698, 1167 |
| BssAI | RCCGGY | 3 cut(s) | 120, 572, 1576 |
| BssMI | GATC | 4 cut(s) | 141, 221, 580, 1287 |
| BssNAI | GTATAC | 1 cut(s) | 291 |
| BssNI | GRCGYC | 4 cut(s) | 1079, 1212, 1536, 1556 |
| BssT1I | CCWWGG | 2 cut(s) | 381, 1188 |
| Bst1107I | GTATAC | 1 cut(s) | 291 |
| Bst4CI | ACNGT | 6 cut(s) | 43, 203, 451, 550, 663, 918 |
| Bst6I | CTCTTC | 3 cut(s) | 303, 774, 953 |
| BstACI | GRCGYC | 4 cut(s) | 1079, 1212, 1536, 1556 |
| BstBAI | YACGTR | 1 cut(s) | 1715 |
| BstBI | TTCGAA | 1 cut(s) | 842 |
| BstDEI | CTNAG | 9 cut(s) | 152, 258, 476, 645, 762, 1109, 1317, 1403, 1581 |
| BstDSI | CCRYGG | 1 cut(s) | 381 |
| BstEII | GGTNACC | 1 cut(s) | 1393 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1097, 1668 |
| BstF5I | GGATG | 4 cut(s) | 541, 627, 1368, 1660 |
| BstFNI | CGCG | 8 cut(s) | 301, 303, 318, 363, 599, 776, 997, 1266 |
| BstH2I | RGCGCY | 7 cut(s) | 88, 134, 476, 597, 754, 1317, 1539 |
| BstKTI | GATC | 4 cut(s) | 144, 224, 583, 1290 |
| BstMAI | GTCTC | 7 cut(s) | 458, 525, 771, 1133, 1153, 1273, 1592 |
| BstMBI | GATC | 4 cut(s) | 141, 221, 580, 1287 |
| BstMCI | CGRYCG | 3 cut(s) | 242, 573, 1431 |
| BstNSI | RCATGY | 1 cut(s) | 1522 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1604 |
| BstPI | GGTNACC | 1 cut(s) | 1393 |
| BstSFI | CTRYAG | 3 cut(s) | 195, 1298, 1623 |
| BstSLI | GKGCMC | 2 cut(s) | 811, 1504 |
| BstUI | CGCG | 8 cut(s) | 301, 303, 318, 363, 599, 776, 997, 1266 |
| BstV1I | GCAGC | 8 cut(s) | 80, 823, 1099, 1284, 1354, 1411, 1609, 1653 |
| BstV2I | GAAGAC | 2 cut(s) | 851, 1478 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 638 |
| BstZ17I | GTATAC | 1 cut(s) | 291 |
| BstZI | CGGCCG | 2 cut(s) | 570, 1428 |
| BtgI | CCRYGG | 1 cut(s) | 381 |
| BtgZI | GCGATG | 1 cut(s) | 431 |
| BtsCI | GGATG | 4 cut(s) | 541, 627, 1368, 1660 |
| BtsIMutI | CAGTG | 2 cut(s) | 705, 1475 |
| BtuMI | TCGCGA | 1 cut(s) | 363 |
| BveI | ACCTGC | 1 cut(s) | 1616 |
| CciNI | GCGGCCGC | 1 cut(s) | 1428 |
| Cfr10I | RCCGGY | 3 cut(s) | 120, 572, 1576 |
| Cfr9I | CCCGGG | 3 cut(s) | 602, 1503, 1740 |
| CseI | GACGC | 7 cut(s) | 178, 307, 374, 782, 1068, 1255, 1545 |
| Csp6I | GTAC | 4 cut(s) | 211, 1419, 1571, 1629 |
| CviAII | CATG | 3 cut(s) | 163, 382, 1519 |
| CviQI | GTAC | 4 cut(s) | 211, 1419, 1571, 1629 |
| DdeI | CTNAG | 9 cut(s) | 152, 258, 476, 645, 762, 1109, 1317, 1403, 1581 |
| DinI | GGCGCC | 1 cut(s) | 1537 |
| DpnI | GATC | 4 cut(s) | 143, 223, 582, 1289 |
| DpnII | GATC | 4 cut(s) | 141, 221, 580, 1287 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 763 |
| DriI | GACNNNNNGTC | 1 cut(s) | 163 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 763 |
| EaeI | YGGCCR | 5 cut(s) | 570, 1185, 1233, 1365, 1428 |
| EagI | CGGCCG | 2 cut(s) | 570, 1428 |
| Eam1104I | CTCTTC | 3 cut(s) | 303, 774, 953 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 163 |
| EarI | CTCTTC | 3 cut(s) | 303, 774, 953 |
| EciI | GGCGGA | 6 cut(s) | 62, 330, 407, 1160, 1208, 1342 |
| Ecl136II | GAGCTC | 1 cut(s) | 1108 |
| EclXI | CGGCCG | 2 cut(s) | 570, 1428 |
| Eco130I | CCWWGG | 2 cut(s) | 381, 1188 |
| Eco24I | GRGCYC | 3 cut(s) | 811, 1110, 1504 |
| Eco31I | GGTCTC | 1 cut(s) | 1133 |
| Eco32I | GATATC | 1 cut(s) | 1120 |
| Eco47I | GGWCC | 6 cut(s) | 180, 385, 561, 641, 714, 1674 |
| Eco52I | CGGCCG | 2 cut(s) | 570, 1428 |
| Eco53kI | GAGCTC | 1 cut(s) | 1108 |
| Eco57I | CTGAAG | 2 cut(s) | 1009, 1431 |
| Eco72I | CACGTG | 1 cut(s) | 1715 |
| Eco88I | CYCGRG | 5 cut(s) | 461, 602, 1503, 1636, 1740 |
| Eco91I | GGTNACC | 1 cut(s) | 1393 |
| EcoICRI | GAGCTC | 1 cut(s) | 1108 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1097, 1668 |
| EcoO109I | RGGNCCY | 6 cut(s) | 35, 180, 561, 807, 1500, 1674 |
| EcoO65I | GGTNACC | 1 cut(s) | 1393 |
| EcoRV | GATATC | 1 cut(s) | 1120 |
| EcoT14I | CCWWGG | 2 cut(s) | 381, 1188 |
| EcoT38I | GRGCYC | 3 cut(s) | 811, 1110, 1504 |
| EgeI | GGCGCC | 1 cut(s) | 1537 |
| EheI | GGCGCC | 1 cut(s) | 1537 |
| ErhI | CCWWGG | 2 cut(s) | 381, 1188 |
| Esp3I | CGTCTC | 2 cut(s) | 458, 1273 |
| FaeI | CATG | 3 cut(s) | 166, 385, 1522 |
| FaqI | GGGAC | 8 cut(s) | 170, 193, 294, 574, 1068, 1199, 1574, 1660 |
| FatI | CATG | 3 cut(s) | 162, 381, 1518 |
| FblI | GTMKAC | 2 cut(s) | 290, 1515 |
| FokI | GGATG | 4 cut(s) | 548, 634, 1375, 1647 |
| FriOI | GRGCYC | 3 cut(s) | 811, 1110, 1504 |
| FspBI | CTAG | 1 cut(s) | 893 |
| GsaI | CCCAGC | 7 cut(s) | 445, 726, 971, 1385, 1500, 1622, 1681 |
| GsuI | CTGGAG | 3 cut(s) | 761, 1115, 1748 |
| HaeII | RGCGCY | 7 cut(s) | 88, 134, 476, 597, 754, 1317, 1539 |
| HgaI | GACGC | 7 cut(s) | 178, 307, 374, 782, 1068, 1255, 1545 |
| Hin1I | GRCGYC | 4 cut(s) | 1079, 1212, 1536, 1556 |
| Hin1II | CATG | 3 cut(s) | 166, 385, 1522 |
| HinfI | GANTC | 7 cut(s) | 115, 148, 158, 358, 764, 896, 1349 |
| HphI | GGTGA | 4 cut(s) | 466, 542, 718, 1400 |
| Hpy166II | GTNNAC | 4 cut(s) | 109, 291, 457, 1516 |
| Hpy188III | TCNNGA | 5 cut(s) | 145, 362, 770, 1132, 1727 |
| Hpy8I | GTNNAC | 4 cut(s) | 109, 291, 457, 1516 |
| Hpy99I | CGWCG | 6 cut(s) | 194, 368, 667, 1006, 1084, 1705 |
| HpyCH4III | ACNGT | 6 cut(s) | 43, 203, 451, 550, 663, 918 |
| HpyCH4IV | ACGT | 4 cut(s) | 467, 1212, 1569, 1714 |
| HpyCH4V | TGCA | 8 cut(s) | 96, 553, 914, 960, 1208, 1300, 1342, 1625 |
| HpyF3I | CTNAG | 9 cut(s) | 152, 258, 476, 645, 762, 1109, 1317, 1403, 1581 |
| HpySE526I | ACGT | 4 cut(s) | 467, 1212, 1569, 1714 |
| Hsp92I | GRCGYC | 4 cut(s) | 1079, 1212, 1536, 1556 |
| Hsp92II | CATG | 3 cut(s) | 166, 385, 1522 |
| KasI | GGCGCC | 1 cut(s) | 1535 |
| KflI | GGGWCCC | 2 cut(s) | 561, 1674 |
| KroI | GCCGGC | 3 cut(s) | 120, 572, 1576 |
| KroNI | GCCGGC | 3 cut(s) | 122, 574, 1578 |
| Kzo9I | GATC | 4 cut(s) | 141, 221, 580, 1287 |
| LmnI | GCTCC | 6 cut(s) | 128, 616, 934, 1252, 1279, 1303 |
| Lsp1109I | GCAGC | 8 cut(s) | 80, 823, 1099, 1284, 1354, 1411, 1609, 1653 |
| LweI | GCATC | 4 cut(s) | 492, 498, 612, 862 |
| MaeI | CTAG | 1 cut(s) | 893 |
| MaeII | ACGT | 4 cut(s) | 467, 1212, 1569, 1714 |
| MaeIII | GTNAC | 7 cut(s) | 679, 706, 1199, 1244, 1393, 1557, 1710 |
| MalI | GATC | 4 cut(s) | 143, 223, 582, 1289 |
| MbiI | CCGCTC | 6 cut(s) | 278, 619, 937, 1177, 1255, 1306 |
| MboI | GATC | 4 cut(s) | 141, 221, 580, 1287 |
| MfeI | CAATTG | 1 cut(s) | 1008 |
| MhlI | GDGCHC | 3 cut(s) | 811, 1110, 1504 |
| MlsI | TGGCCA | 2 cut(s) | 1187, 1235 |
| MluCI | AATT | 3 cut(s) | 403, 1008, 1095 |
| MluI | ACGCGT | 1 cut(s) | 1264 |
| MluNI | TGGCCA | 2 cut(s) | 1187, 1235 |
| Mly113I | GGCGCC | 1 cut(s) | 1536 |
| MlyI | GAGTC | 6 cut(s) | 124, 152, 367, 773, 890, 1358 |
| MmeI | TCCRAC | 4 cut(s) | 289, 645, 1257, 1683 |
| Mox20I | TGGCCA | 2 cut(s) | 1187, 1235 |
| MroNI | GCCGGC | 3 cut(s) | 120, 572, 1576 |
| MroXI | GAANNNNTTC | 1 cut(s) | 54 |
| MscI | TGGCCA | 2 cut(s) | 1187, 1235 |
| MseI | TTAA | 2 cut(s) | 252, 1467 |
| MslI | CAYNNNNRTG | 3 cut(s) | 528, 636, 1337 |
| Msp20I | TGGCCA | 2 cut(s) | 1187, 1235 |
| MspA1I | CMGCKG | 6 cut(s) | 722, 1345, 1424, 1436, 1622, 1644 |
| MteI | GCGCNGCGC | 1 cut(s) | 597 |
| MunI | CAATTG | 1 cut(s) | 1008 |
| MvnI | CGCG | 8 cut(s) | 301, 303, 318, 363, 599, 776, 997, 1266 |
| NaeI | GCCGGC | 3 cut(s) | 122, 574, 1578 |
| NarI | GGCGCC | 1 cut(s) | 1536 |
| NcoI | CCATGG | 1 cut(s) | 381 |
| NdeII | GATC | 4 cut(s) | 141, 221, 580, 1287 |
| NgoMIV | GCCGGC | 3 cut(s) | 120, 572, 1576 |
| NlaIII | CATG | 3 cut(s) | 166, 385, 1522 |
| NmeAIII | GCCGAG | 3 cut(s) | 113, 451, 931 |
| NmuCI | GTSAC | 6 cut(s) | 679, 706, 1199, 1244, 1557, 1710 |
| NotI | GCGGCCGC | 1 cut(s) | 1428 |
| NruI | TCGCGA | 1 cut(s) | 363 |
| NspI | RCATGY | 1 cut(s) | 1522 |
| NspV | TTCGAA | 1 cut(s) | 842 |
| OliI | CACNNNNGTG | 1 cut(s) | 636 |
| PciI | ACATGT | 1 cut(s) | 1518 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1068 |
| PdiI | GCCGGC | 3 cut(s) | 122, 574, 1578 |
| PdmI | GAANNNNTTC | 1 cut(s) | 54 |
| PfeI | GAWTC | 1 cut(s) | 148 |
| PleI | GAGTC | 6 cut(s) | 123, 152, 366, 772, 890, 1357 |
| PluTI | GGCGCC | 1 cut(s) | 1539 |
| PmaCI | CACGTG | 1 cut(s) | 1715 |
| PmlI | CACGTG | 1 cut(s) | 1715 |
| PpsI | GAGTC | 6 cut(s) | 123, 152, 366, 772, 890, 1357 |
| Ppu21I | YACGTR | 1 cut(s) | 1715 |
| PpuMI | RGGWCCY | 3 cut(s) | 180, 561, 1674 |
| PscI | ACATGT | 1 cut(s) | 1518 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1604 |
| Psp124BI | GAGCTC | 1 cut(s) | 1110 |
| Psp5II | RGGWCCY | 3 cut(s) | 180, 561, 1674 |
| PspCI | CACGTG | 1 cut(s) | 1715 |
| PspEI | GGTNACC | 1 cut(s) | 1393 |
| PspFI | CCCAGC | 7 cut(s) | 441, 722, 967, 1381, 1496, 1618, 1677 |
| PspOMI | GGGCCC | 2 cut(s) | 807, 1500 |
| PspPPI | RGGWCCY | 3 cut(s) | 180, 561, 1674 |
| PstI | CTGCAG | 2 cut(s) | 1302, 1627 |
| PvuII | CAGCTG | 1 cut(s) | 1622 |
| RruI | TCGCGA | 1 cut(s) | 363 |
| RsaI | GTAC | 4 cut(s) | 212, 1420, 1572, 1630 |
| RsaNI | GTAC | 4 cut(s) | 211, 1419, 1571, 1629 |
| RseI | CAYNNNNRTG | 3 cut(s) | 528, 636, 1337 |
| SacI | GAGCTC | 1 cut(s) | 1110 |
| SaqAI | TTAA | 2 cut(s) | 252, 1467 |
| Sau3AI | GATC | 4 cut(s) | 141, 221, 580, 1287 |
| SchI | GAGTC | 6 cut(s) | 124, 152, 367, 773, 890, 1358 |
| SduI | GDGCHC | 3 cut(s) | 811, 1110, 1504 |
| SfaNI | GCATC | 4 cut(s) | 492, 498, 612, 862 |
| SfcI | CTRYAG | 3 cut(s) | 195, 1298, 1623 |
| SfoI | GGCGCC | 1 cut(s) | 1537 |
| SfuI | TTCGAA | 1 cut(s) | 842 |
| SinI | GGWCC | 6 cut(s) | 180, 385, 561, 641, 714, 1674 |
| SmaI | CCCGGG | 3 cut(s) | 604, 1505, 1742 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 528, 636, 1337 |
| SmlI | CTYRAG | 1 cut(s) | 112 |
| SmoI | CTYRAG | 1 cut(s) | 112 |
| SrfI | GCCCGGGC | 2 cut(s) | 604, 1505 |
| Sse9I | AATT | 3 cut(s) | 403, 1008, 1095 |
| SspDI | GGCGCC | 1 cut(s) | 1535 |
| SspMI | CTAG | 1 cut(s) | 893 |
| SstI | GAGCTC | 1 cut(s) | 1110 |
| StyI | CCWWGG | 2 cut(s) | 381, 1188 |
| TaaI | ACNGT | 6 cut(s) | 43, 203, 451, 550, 663, 918 |
| TaiI | ACGT | 4 cut(s) | 470, 1215, 1572, 1717 |
| TaqI | TCGA | 6 cut(s) | 144, 224, 274, 356, 842, 1082 |
| TaqII | GACCGA | 1 cut(s) | 1496 |
| TasI | AATT | 3 cut(s) | 403, 1008, 1095 |
| TfiI | GAWTC | 1 cut(s) | 148 |
| Tru1I | TTAA | 2 cut(s) | 252, 1467 |
| Tru9I | TTAA | 2 cut(s) | 252, 1467 |
| TscAI | CASTG | 2 cut(s) | 705, 1482 |
| TseFI | GTSAC | 6 cut(s) | 679, 706, 1199, 1244, 1557, 1710 |
| TseI | GCWGC | 8 cut(s) | 93, 836, 1087, 1297, 1342, 1424, 1622, 1641 |
| Tsp45I | GTSAC | 6 cut(s) | 679, 706, 1199, 1244, 1557, 1710 |
| TspDTI | ATGAA | 4 cut(s) | 17, 243, 1275, 1388 |
| TspMI | CCCGGG | 3 cut(s) | 602, 1503, 1740 |
| TspRI | CASTG | 2 cut(s) | 705, 1482 |
| VpaK11BI | GGWCC | 6 cut(s) | 180, 385, 561, 641, 714, 1674 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1097, 1668 |
| XceI | RCATGY | 1 cut(s) | 1522 |
| XmaI | CCCGGG | 3 cut(s) | 602, 1503, 1740 |
| XmiI | GTMKAC | 2 cut(s) | 290, 1515 |
| XmnI | GAANNNNTTC | 1 cut(s) | 54 |
| XspI | CTAG | 1 cut(s) | 893 |
| ZraI | GACGTC | 1 cut(s) | 1213 |