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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 779 |
| Acc65I | GGTACC | 1 cut(s) | 1847 |
| AccB1I | GGYRCC | 5 cut(s) | 422, 1323, 1595, 1724, 1847 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1111, 1641 |
| AccBSI | CCGCTC | 3 cut(s) | 1321, 1731, 1749 |
| AccI | GTMKAC | 2 cut(s) | 729, 906 |
| AccII | CGCG | 4 cut(s) | 377, 487, 663, 1694 |
| AclWI | GGATC | 4 cut(s) | 508, 521, 1407, 1426 |
| AcoI | YGGCCR | 2 cut(s) | 204, 1602 |
| AcsI | RAATTY | 1 cut(s) | 1061 |
| AcuI | CTGAAG | 4 cut(s) | 1259, 1415, 1421, 1485 |
| AcyI | GRCGYC | 3 cut(s) | 1324, 1632, 1859 |
| AfaI | GTAC | 2 cut(s) | 169, 1849 |
| AgsI | TTSAA | 3 cut(s) | 446, 1157, 1621 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 455, 1896 |
| AjnI | CCWGG | 9 cut(s) | 149, 241, 415, 462, 504, 636, 681, 1236, 1497 |
| AleI | CACNNNNGTG | 1 cut(s) | 1836 |
| Alw21I | GWGCWC | 1 cut(s) | 1145 |
| Alw26I | GTCTC | 4 cut(s) | 483, 941, 1286, 1810 |
| AlwI | GGATC | 4 cut(s) | 508, 521, 1407, 1426 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1111, 1237 |
| Ama87I | CYCGRG | 2 cut(s) | 1359, 1922 |
| ApeKI | GCWGC | 8 cut(s) | 50, 92, 256, 355, 857, 1220, 1231, 1349 |
| ApoI | RAATTY | 1 cut(s) | 1061 |
| AscI | GGCGCGCC | 1 cut(s) | 375 |
| Asp718I | GGTACC | 1 cut(s) | 1847 |
| AspLEI | GCGC | 8 cut(s) | 92, 377, 379, 665, 780, 862, 1326, 1687 |
| AspS9I | GGNCC | 9 cut(s) | 191, 472, 658, 712, 803, 958, 985, 1013, 1881 |
| AsuC2I | CCSGG | 1 cut(s) | 935 |
| AsuHPI | GGTGA | 3 cut(s) | 127, 402, 451 |
| AsuNHI | GCTAGC | 1 cut(s) | 95 |
| AvaI | CYCGRG | 2 cut(s) | 1359, 1922 |
| AvaII | GGWCC | 4 cut(s) | 472, 658, 958, 1881 |
| BaeGI | GKGCMC | 3 cut(s) | 427, 1188, 1729 |
| BalI | TGGCCA | 1 cut(s) | 1604 |
| BamHI | GGATCC | 1 cut(s) | 513 |
| BanI | GGYRCC | 5 cut(s) | 422, 1323, 1595, 1724, 1847 |
| BanII | GRGCYC | 3 cut(s) | 604, 1203, 1868 |
| BauI | CACGAG | 2 cut(s) | 317, 597 |
| BbsI | GAAGAC | 2 cut(s) | 725, 1265 |
| Bbv12I | GWGCWC | 1 cut(s) | 1145 |
| BbvCI | CCTCAGC | 3 cut(s) | 1506, 1719, 1950 |
| BbvI | GCAGC | 8 cut(s) | 37, 104, 243, 342, 844, 1232, 1243, 1336 |
| BccI | CCATC | 4 cut(s) | 104, 763, 1643, 1838 |
| BceAI | ACGGC | 3 cut(s) | 194, 826, 1881 |
| BciT130I | CCWGG | 9 cut(s) | 151, 243, 417, 464, 506, 638, 683, 1238, 1499 |
| BcnI | CCSGG | 1 cut(s) | 935 |
| BcoDI | GTCTC | 4 cut(s) | 483, 941, 1286, 1810 |
| BfaI | CTAG | 5 cut(s) | 30, 96, 545, 702, 1373 |
| BfmI | CTRYAG | 1 cut(s) | 1168 |
| BfoI | RGCGCY | 1 cut(s) | 1327 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 301, 810, 1535 |
| BglII | AGATCT | 1 cut(s) | 547 |
| Bme18I | GGWCC | 4 cut(s) | 472, 658, 958, 1881 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 455, 1896 |
| BmeT110I | CYCGRG | 2 cut(s) | 1359, 1922 |
| BmgT120I | GGNCC | 9 cut(s) | 191, 472, 658, 712, 803, 958, 985, 1013, 1881 |
| BmrI | ACTGGG | 1 cut(s) | 1020 |
| BmsI | GCATC | 9 cut(s) | 34, 375, 796, 1303, 1336, 1345, 1359, 1661, 1933 |
| BmtI | GCTAGC | 1 cut(s) | 99 |
| BmuI | ACTGGG | 1 cut(s) | 1020 |
| BoxI | GACNNNNGTC | 1 cut(s) | 725 |
| BpiI | GAAGAC | 2 cut(s) | 725, 1265 |
| BpmI | CTGGAG | 2 cut(s) | 704, 947 |
| Bpu10I | CCTNAGC | 6 cut(s) | 1077, 1253, 1506, 1719, 1732, 1950 |
| BpuMI | CCSGG | 1 cut(s) | 935 |
| Bsa29I | ATCGAT | 1 cut(s) | 1638 |
| BsaHI | GRCGYC | 3 cut(s) | 1324, 1632, 1859 |
| BsaI | GGTCTC | 3 cut(s) | 941, 1286, 1810 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 677, 707, 1771, 1801 |
| Bse118I | RCCGGY | 1 cut(s) | 563 |
| Bse1I | ACTGG | 3 cut(s) | 747, 863, 1015 |
| Bse3DI | GCAATG | 2 cut(s) | 350, 853 |
| BseBI | CCWGG | 9 cut(s) | 151, 243, 417, 464, 506, 638, 683, 1238, 1499 |
| BseCI | ATCGAT | 1 cut(s) | 1638 |
| BseGI | GGATG | 4 cut(s) | 49, 390, 1318, 1705 |
| BseMI | GCAATG | 2 cut(s) | 350, 853 |
| BseNI | ACTGG | 3 cut(s) | 747, 863, 1015 |
| BsePI | GCGCGC | 1 cut(s) | 375 |
| BseRI | GAGGAG | 1 cut(s) | 1274 |
| BseSI | GKGCMC | 3 cut(s) | 427, 1188, 1729 |
| BseXI | GCAGC | 8 cut(s) | 37, 104, 243, 342, 844, 1232, 1243, 1336 |
| BseYI | CCCAGC | 2 cut(s) | 567, 1111 |
| BsgI | GTGCAG | 1 cut(s) | 845 |
| Bsh1236I | CGCG | 4 cut(s) | 377, 487, 663, 1694 |
| Bsh1285I | CGRYCG | 2 cut(s) | 263, 1032 |
| BshNI | GGYRCC | 5 cut(s) | 422, 1323, 1595, 1724, 1847 |
| BshVI | ATCGAT | 1 cut(s) | 1638 |
| BsiEI | CGRYCG | 2 cut(s) | 263, 1032 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1145 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1359, 1922 |
| BsiSI | CCGG | 4 cut(s) | 564, 935, 1016, 1760 |
| BslFI | GGGAC | 2 cut(s) | 485, 836 |
| BsmAI | GTCTC | 4 cut(s) | 483, 941, 1286, 1810 |
| BsmBI | CGTCTC | 1 cut(s) | 483 |
| BsmFI | GGGAC | 2 cut(s) | 485, 836 |
| BsmI | GAATGC | 1 cut(s) | 1687 |
| Bso31I | GGTCTC | 3 cut(s) | 941, 1286, 1810 |
| BsoBI | CYCGRG | 2 cut(s) | 1359, 1922 |
| Bsp1286I | GDGCHC | 7 cut(s) | 427, 604, 1145, 1188, 1203, 1729, 1868 |
| Bsp19I | CCATGG | 2 cut(s) | 654, 1599 |
| BspDI | ATCGAT | 1 cut(s) | 1638 |
| BspFNI | CGCG | 4 cut(s) | 377, 487, 663, 1694 |
| BspOI | GCTAGC | 1 cut(s) | 99 |
| BspPI | GGATC | 4 cut(s) | 508, 521, 1407, 1426 |
| BspT107I | GGYRCC | 5 cut(s) | 422, 1323, 1595, 1724, 1847 |
| BspTNI | GGTCTC | 3 cut(s) | 941, 1286, 1810 |
| BsrBI | CCGCTC | 3 cut(s) | 1321, 1731, 1749 |
| BsrDI | GCAATG | 2 cut(s) | 350, 853 |
| BsrFI | RCCGGY | 1 cut(s) | 563 |
| BsrI | ACTGG | 3 cut(s) | 747, 863, 1015 |
| BssAI | RCCGGY | 1 cut(s) | 563 |
| BssHII | GCGCGC | 1 cut(s) | 375 |
| BssNAI | GTATAC | 1 cut(s) | 907 |
| BssNI | GRCGYC | 3 cut(s) | 1324, 1632, 1859 |
| BssSI | CACGAG | 2 cut(s) | 317, 597 |
| BssT1I | CCWWGG | 5 cut(s) | 380, 654, 1296, 1332, 1599 |
| Bst1107I | GTATAC | 1 cut(s) | 907 |
| Bst2BI | CACGAG | 2 cut(s) | 317, 597 |
| Bst2UI | CCWGG | 9 cut(s) | 151, 243, 417, 464, 506, 638, 683, 1238, 1499 |
| Bst4CI | ACNGT | 7 cut(s) | 167, 202, 576, 1003, 1161, 1304, 1617 |
| Bst6I | CTCTTC | 1 cut(s) | 489 |
| BstACI | GRCGYC | 3 cut(s) | 1324, 1632, 1859 |
| BstDSI | CCRYGG | 5 cut(s) | 517, 654, 768, 819, 1599 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1331, 1394 |
| BstF5I | GGATG | 4 cut(s) | 49, 390, 1318, 1705 |
| BstFNI | CGCG | 4 cut(s) | 377, 487, 663, 1694 |
| BstH2I | RGCGCY | 1 cut(s) | 1327 |
| BstHHI | GCGC | 8 cut(s) | 92, 377, 379, 665, 780, 862, 1326, 1687 |
| BstMAI | GTCTC | 4 cut(s) | 483, 941, 1286, 1810 |
| BstMCI | CGRYCG | 2 cut(s) | 263, 1032 |
| BstNI | CCWGG | 9 cut(s) | 151, 243, 417, 464, 506, 638, 683, 1238, 1499 |
| BstNSI | RCATGY | 1 cut(s) | 308 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 725 |
| BstSFI | CTRYAG | 1 cut(s) | 1168 |
| BstSLI | GKGCMC | 3 cut(s) | 427, 1188, 1729 |
| BstUI | CGCG | 4 cut(s) | 377, 487, 663, 1694 |
| BstV1I | GCAGC | 8 cut(s) | 37, 104, 243, 342, 844, 1232, 1243, 1336 |
| BstV2I | GAAGAC | 2 cut(s) | 725, 1265 |
| BstX2I | RGATCY | 3 cut(s) | 513, 547, 1431 |
| BstYI | RGATCY | 3 cut(s) | 513, 547, 1431 |
| BstZ17I | GTATAC | 1 cut(s) | 907 |
| Bsu15I | ATCGAT | 1 cut(s) | 1638 |
| BsuTUI | ATCGAT | 1 cut(s) | 1638 |
| BtgI | CCRYGG | 5 cut(s) | 517, 654, 768, 819, 1599 |
| BtsCI | GGATG | 4 cut(s) | 49, 390, 1318, 1705 |
| BtsI | GCAGTG | 1 cut(s) | 1916 |
| BtsIMutI | CAGTG | 2 cut(s) | 1008, 1916 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1111, 1237 |
| CfoI | GCGC | 8 cut(s) | 92, 377, 379, 665, 780, 862, 1326, 1687 |
| Cfr10I | RCCGGY | 1 cut(s) | 563 |
| Cfr13I | GGNCC | 9 cut(s) | 191, 472, 658, 712, 803, 958, 985, 1013, 1881 |
| ClaI | ATCGAT | 1 cut(s) | 1638 |
| CseI | GACGC | 6 cut(s) | 273, 493, 793, 1640, 1745, 1867 |
| Csp6I | GTAC | 2 cut(s) | 168, 1848 |
| CviAII | CATG | 9 cut(s) | 305, 455, 655, 781, 1205, 1573, 1600, 1811, 1956 |
| CviQI | GTAC | 2 cut(s) | 168, 1848 |
| DinI | GGCGCC | 1 cut(s) | 1325 |
| DriI | GACNNNNNGTC | 2 cut(s) | 455, 1896 |
| EaeI | YGGCCR | 2 cut(s) | 204, 1602 |
| Eam1104I | CTCTTC | 1 cut(s) | 489 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 455, 1896 |
| EarI | CTCTTC | 1 cut(s) | 489 |
| EciI | GGCGGA | 4 cut(s) | 224, 542, 1304, 1778 |
| Eco130I | CCWWGG | 5 cut(s) | 380, 654, 1296, 1332, 1599 |
| Eco147I | AGGCCT | 1 cut(s) | 1389 |
| Eco24I | GRGCYC | 3 cut(s) | 604, 1203, 1868 |
| Eco31I | GGTCTC | 3 cut(s) | 941, 1286, 1810 |
| Eco47I | GGWCC | 4 cut(s) | 472, 658, 958, 1881 |
| Eco57I | CTGAAG | 4 cut(s) | 1259, 1415, 1421, 1485 |
| Eco88I | CYCGRG | 2 cut(s) | 1359, 1922 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1331, 1394 |
| EcoO109I | RGGNCCY | 3 cut(s) | 472, 958, 985 |
| EcoRI | GAATTC | 1 cut(s) | 1061 |
| EcoRII | CCWGG | 9 cut(s) | 149, 241, 415, 462, 504, 636, 681, 1236, 1497 |
| EcoT14I | CCWWGG | 5 cut(s) | 380, 654, 1296, 1332, 1599 |
| EcoT38I | GRGCYC | 3 cut(s) | 604, 1203, 1868 |
| EgeI | GGCGCC | 1 cut(s) | 1325 |
| EheI | GGCGCC | 1 cut(s) | 1325 |
| ErhI | CCWWGG | 5 cut(s) | 380, 654, 1296, 1332, 1599 |
| Esp3I | CGTCTC | 1 cut(s) | 483 |
| FaeI | CATG | 9 cut(s) | 308, 458, 658, 784, 1208, 1576, 1603, 1814, 1959 |
| FaqI | GGGAC | 2 cut(s) | 485, 836 |
| FatI | CATG | 9 cut(s) | 304, 454, 654, 780, 1204, 1572, 1599, 1810, 1955 |
| FauI | CCCGC | 8 cut(s) | 57, 178, 292, 823, 1434, 1669, 1699, 1736 |
| FauNDI | CATATG | 1 cut(s) | 1054 |
| FblI | GTMKAC | 2 cut(s) | 729, 906 |
| FokI | GGATG | 4 cut(s) | 56, 397, 1325, 1712 |
| FriOI | GRGCYC | 3 cut(s) | 604, 1203, 1868 |
| FspBI | CTAG | 5 cut(s) | 30, 96, 545, 702, 1373 |
| FspI | TGCGCA | 1 cut(s) | 779 |
| GlaI | GCGC | 8 cut(s) | 91, 376, 378, 664, 779, 861, 1325, 1686 |
| GsaI | CCCAGC | 2 cut(s) | 571, 1115 |
| GsuI | CTGGAG | 2 cut(s) | 704, 947 |
| HaeII | RGCGCY | 1 cut(s) | 1327 |
| HapII | CCGG | 4 cut(s) | 564, 935, 1016, 1760 |
| HgaI | GACGC | 6 cut(s) | 273, 493, 793, 1640, 1745, 1867 |
| HhaI | GCGC | 8 cut(s) | 92, 377, 379, 665, 780, 862, 1326, 1687 |
| Hin1I | GRCGYC | 3 cut(s) | 1324, 1632, 1859 |
| Hin1II | CATG | 9 cut(s) | 308, 458, 658, 784, 1208, 1576, 1603, 1814, 1959 |
| Hin6I | GCGC | 8 cut(s) | 90, 375, 377, 663, 778, 860, 1324, 1685 |
| HinP1I | GCGC | 8 cut(s) | 90, 375, 377, 663, 778, 860, 1324, 1685 |
| HincII | GTYRAC | 3 cut(s) | 214, 730, 940 |
| HindII | GTYRAC | 3 cut(s) | 214, 730, 940 |
| HinfI | GANTC | 8 cut(s) | 143, 401, 407, 450, 608, 1040, 1134, 1667 |
| HpaII | CCGG | 4 cut(s) | 564, 935, 1016, 1760 |
| HphI | GGTGA | 3 cut(s) | 127, 402, 451 |
| Hpy166II | GTNNAC | 4 cut(s) | 214, 730, 907, 940 |
| Hpy188III | TCNNGA | 6 cut(s) | 20, 220, 437, 481, 618, 1066 |
| Hpy8I | GTNNAC | 4 cut(s) | 214, 730, 907, 940 |
| Hpy99I | CGWCG | 6 cut(s) | 221, 619, 731, 812, 884, 924 |
| HpyCH4III | ACNGT | 7 cut(s) | 167, 202, 576, 1003, 1161, 1304, 1617 |
| HpyCH4IV | ACGT | 2 cut(s) | 216, 726 |
| HpyCH4V | TGCA | 4 cut(s) | 316, 388, 826, 1024 |
| HpySE526I | ACGT | 2 cut(s) | 216, 726 |
| Hsp92I | GRCGYC | 3 cut(s) | 1324, 1632, 1859 |
| Hsp92II | CATG | 9 cut(s) | 308, 458, 658, 784, 1208, 1576, 1603, 1814, 1959 |
| HspAI | GCGC | 8 cut(s) | 90, 375, 377, 663, 778, 860, 1324, 1685 |
| KasI | GGCGCC | 1 cut(s) | 1323 |
| KpnI | GGTACC | 1 cut(s) | 1851 |
| LmnI | GCTCC | 6 cut(s) | 107, 833, 1072, 1206, 1282, 1318 |
| Lsp1109I | GCAGC | 8 cut(s) | 37, 104, 243, 342, 844, 1232, 1243, 1336 |
| LweI | GCATC | 9 cut(s) | 34, 375, 796, 1303, 1336, 1345, 1359, 1661, 1933 |
| MaeI | CTAG | 5 cut(s) | 30, 96, 545, 702, 1373 |
| MaeII | ACGT | 2 cut(s) | 216, 726 |
| MaeIII | GTNAC | 7 cut(s) | 161, 340, 408, 457, 892, 997, 1003 |
| MbiI | CCGCTC | 3 cut(s) | 1321, 1731, 1749 |
| MboII | GAAGA | 6 cut(s) | 506, 730, 982, 1084, 1265, 1483 |
| MfeI | CAATTG | 1 cut(s) | 590 |
| MflI | RGATCY | 3 cut(s) | 513, 547, 1431 |
| MhlI | GDGCHC | 7 cut(s) | 427, 604, 1145, 1188, 1203, 1729, 1868 |
| MlsI | TGGCCA | 1 cut(s) | 1604 |
| MluCI | AATT | 5 cut(s) | 538, 590, 676, 1061, 1768 |
| MluNI | TGGCCA | 1 cut(s) | 1604 |
| Mly113I | GGCGCC | 1 cut(s) | 1324 |
| MlyI | GAGTC | 5 cut(s) | 152, 416, 444, 1034, 1676 |
| MmeI | TCCRAC | 2 cut(s) | 847, 1658 |
| Mox20I | TGGCCA | 1 cut(s) | 1604 |
| MscI | TGGCCA | 1 cut(s) | 1604 |
| MseI | TTAA | 1 cut(s) | 1545 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1836 |
| Msp20I | TGGCCA | 1 cut(s) | 1604 |
| MspI | CCGG | 4 cut(s) | 564, 935, 1016, 1760 |
| MunI | CAATTG | 1 cut(s) | 590 |
| Mva1269I | GAATGC | 1 cut(s) | 1687 |
| MvaI | CCWGG | 9 cut(s) | 151, 243, 417, 464, 506, 638, 683, 1238, 1499 |
| MvnI | CGCG | 4 cut(s) | 377, 487, 663, 1694 |
| NarI | GGCGCC | 1 cut(s) | 1324 |
| NciI | CCSGG | 1 cut(s) | 935 |
| NcoI | CCATGG | 2 cut(s) | 654, 1599 |
| NdeI | CATATG | 1 cut(s) | 1054 |
| NheI | GCTAGC | 1 cut(s) | 95 |
| NlaIII | CATG | 9 cut(s) | 308, 458, 658, 784, 1208, 1576, 1603, 1814, 1959 |
| NmeAIII | GCCGAG | 5 cut(s) | 691, 780, 1154, 1690, 1887 |
| NmuCI | GTSAC | 6 cut(s) | 161, 340, 408, 457, 892, 1003 |
| NsbI | TGCGCA | 1 cut(s) | 779 |
| NspI | RCATGY | 1 cut(s) | 308 |
| OliI | CACNNNNGTG | 1 cut(s) | 1836 |
| PaeI | GCATGC | 1 cut(s) | 308 |
| PalAI | GGCGCGCC | 1 cut(s) | 375 |
| PauI | GCGCGC | 1 cut(s) | 375 |
| PceI | AGGCCT | 1 cut(s) | 1389 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 13, 816, 919 |
| PctI | GAATGC | 1 cut(s) | 1687 |
| PfeI | GAWTC | 3 cut(s) | 401, 608, 1134 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1111, 1641 |
| PfoI | TCCNGGA | 1 cut(s) | 681 |
| Ple19I | CGATCG | 1 cut(s) | 1032 |
| PleI | GAGTC | 5 cut(s) | 151, 415, 444, 1034, 1675 |
| PluTI | GGCGCC | 1 cut(s) | 1327 |
| PpsI | GAGTC | 5 cut(s) | 151, 415, 444, 1034, 1675 |
| PpuMI | RGGWCCY | 2 cut(s) | 472, 958 |
| PshAI | GACNNNNGTC | 1 cut(s) | 725 |
| Psp5II | RGGWCCY | 2 cut(s) | 472, 958 |
| Psp6I | CCWGG | 9 cut(s) | 149, 241, 415, 462, 504, 636, 681, 1236, 1497 |
| PspFI | CCCAGC | 2 cut(s) | 567, 1111 |
| PspGI | CCWGG | 9 cut(s) | 149, 241, 415, 462, 504, 636, 681, 1236, 1497 |
| PspPI | GGNCC | 9 cut(s) | 191, 472, 658, 712, 803, 958, 985, 1013, 1881 |
| PspPPI | RGGWCCY | 2 cut(s) | 472, 958 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1111, 1237 |
| PsuI | RGATCY | 3 cut(s) | 513, 547, 1431 |
| PteI | GCGCGC | 1 cut(s) | 375 |
| PvuI | CGATCG | 1 cut(s) | 1032 |
| PvuII | CAGCTG | 1 cut(s) | 1405 |
| RsaI | GTAC | 2 cut(s) | 169, 1849 |
| RsaNI | GTAC | 2 cut(s) | 168, 1848 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1836 |
| SalI | GTCGAC | 1 cut(s) | 728 |
| SaqAI | TTAA | 1 cut(s) | 1545 |
| Sau96I | GGNCC | 9 cut(s) | 191, 472, 658, 712, 803, 958, 985, 1013, 1881 |
| SchI | GAGTC | 5 cut(s) | 152, 416, 444, 1034, 1676 |
| SduI | GDGCHC | 7 cut(s) | 427, 604, 1145, 1188, 1203, 1729, 1868 |
| SfaNI | GCATC | 9 cut(s) | 34, 375, 796, 1303, 1336, 1345, 1359, 1661, 1933 |
| SfcI | CTRYAG | 1 cut(s) | 1168 |
| SfoI | GGCGCC | 1 cut(s) | 1325 |
| SgsI | GGCGCGCC | 1 cut(s) | 375 |
| SinI | GGWCC | 4 cut(s) | 472, 658, 958, 1881 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1836 |
| SphI | GCATGC | 1 cut(s) | 308 |
| Sse9I | AATT | 5 cut(s) | 538, 590, 676, 1061, 1768 |
| SseBI | AGGCCT | 1 cut(s) | 1389 |
| SspDI | GGCGCC | 1 cut(s) | 1323 |
| SspMI | CTAG | 5 cut(s) | 30, 96, 545, 702, 1373 |
| StuI | AGGCCT | 1 cut(s) | 1389 |
| StyI | CCWWGG | 5 cut(s) | 380, 654, 1296, 1332, 1599 |
| TaaI | ACNGT | 7 cut(s) | 167, 202, 576, 1003, 1161, 1304, 1617 |
| TaiI | ACGT | 2 cut(s) | 219, 729 |
| TaqI | TCGA | 8 cut(s) | 436, 582, 729, 992, 1032, 1095, 1266, 1638 |
| TasI | AATT | 5 cut(s) | 538, 590, 676, 1061, 1768 |
| TfiI | GAWTC | 3 cut(s) | 401, 608, 1134 |
| Tru1I | TTAA | 1 cut(s) | 1545 |
| Tru9I | TTAA | 1 cut(s) | 1545 |
| TscAI | CASTG | 2 cut(s) | 1015, 1923 |
| TseFI | GTSAC | 6 cut(s) | 161, 340, 408, 457, 892, 1003 |
| TseI | GCWGC | 8 cut(s) | 50, 92, 256, 355, 857, 1220, 1231, 1349 |
| Tsp45I | GTSAC | 6 cut(s) | 161, 340, 408, 457, 892, 1003 |
| TspDTI | ATGAA | 2 cut(s) | 266, 1041 |
| TspGWI | ACGGA | 8 cut(s) | 149, 406, 506, 603, 928, 1085, 1325, 1861 |
| TspRI | CASTG | 2 cut(s) | 1015, 1923 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1111, 1641 |
| VpaK11BI | GGWCC | 4 cut(s) | 472, 658, 958, 1881 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1331, 1394 |
| XapI | RAATTY | 1 cut(s) | 1061 |
| XceI | RCATGY | 1 cut(s) | 308 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1234, 1606 |
| XmiI | GTMKAC | 2 cut(s) | 729, 906 |
| XspI | CTAG | 5 cut(s) | 30, 96, 545, 702, 1373 |