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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 673 |
| AccBSI | CCGCTC | 2 cut(s) | 749, 964 |
| AccII | CGCG | 4 cut(s) | 450, 559, 582, 777 |
| AccIII | TCCGGA | 1 cut(s) | 19 |
| AclWI | GGATC | 4 cut(s) | 469, 482, 586, 599 |
| AcoI | YGGCCR | 1 cut(s) | 729 |
| AcsI | RAATTY | 3 cut(s) | 305, 414, 803 |
| AcyI | GRCGYC | 1 cut(s) | 620 |
| AfaI | GTAC | 2 cut(s) | 234, 607 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 111, 284, 360, 401, 429, 434, 713, 799 |
| AgeI | ACCGGT | 1 cut(s) | 876 |
| AgsI | TTSAA | 3 cut(s) | 293, 311, 889 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 190 |
| AleI | CACNNNNGTG | 1 cut(s) | 600 |
| AluBI | AGCT | 4 cut(s) | 99, 296, 535, 970 |
| AluI | AGCT | 4 cut(s) | 99, 296, 535, 970 |
| Alw26I | GTCTC | 2 cut(s) | 330, 659 |
| AlwI | GGATC | 4 cut(s) | 469, 482, 586, 599 |
| Ama87I | CYCGRG | 3 cut(s) | 710, 830, 954 |
| Aor13HI | TCCGGA | 1 cut(s) | 19 |
| AoxI | GGCC | 7 cut(s) | 74, 286, 729, 841, 894, 957, 988 |
| ApeKI | GCWGC | 3 cut(s) | 50, 253, 919 |
| ApoI | RAATTY | 3 cut(s) | 305, 414, 803 |
| AsiGI | ACCGGT | 1 cut(s) | 876 |
| AspLEI | GCGC | 3 cut(s) | 92, 244, 584 |
| AspS9I | GGNCC | 8 cut(s) | 184, 192, 209, 286, 675, 842, 957, 988 |
| AsuC2I | CCSGG | 7 cut(s) | 282, 526, 733, 845, 935, 955, 956 |
| AsuHPI | GGTGA | 4 cut(s) | 127, 267, 316, 873 |
| AsuNHI | GCTAGC | 1 cut(s) | 95 |
| AvaI | CYCGRG | 3 cut(s) | 710, 830, 954 |
| AvaII | GGWCC | 4 cut(s) | 184, 192, 209, 675 |
| BaeGI | GKGCMC | 1 cut(s) | 696 |
| BamHI | GGATCC | 2 cut(s) | 474, 591 |
| BbvI | GCAGC | 3 cut(s) | 37, 265, 906 |
| BccI | CCATC | 5 cut(s) | 104, 183, 354, 380, 632 |
| BceAI | ACGGC | 4 cut(s) | 174, 483, 842, 882 |
| BcnI | CCSGG | 7 cut(s) | 282, 526, 733, 845, 935, 955, 956 |
| BcoDI | GTCTC | 2 cut(s) | 330, 659 |
| BfaI | CTAG | 4 cut(s) | 30, 96, 506, 994 |
| BfoI | RGCGCY | 2 cut(s) | 93, 245 |
| BglII | AGATCT | 1 cut(s) | 508 |
| BisI | GCNGC | 5 cut(s) | 51, 93, 254, 769, 920 |
| BlsI | GCNGC | 5 cut(s) | 52, 94, 255, 770, 921 |
| Bme1390I | CCNGG | 7 cut(s) | 282, 526, 733, 845, 935, 955, 956 |
| Bme18I | GGWCC | 4 cut(s) | 184, 192, 209, 675 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 190 |
| BmeT110I | CYCGRG | 3 cut(s) | 710, 830, 954 |
| BmgT120I | GGNCC | 8 cut(s) | 184, 192, 209, 286, 675, 842, 957, 988 |
| BmiI | GGNNCC | 6 cut(s) | 287, 476, 593, 676, 677, 705 |
| BmrFI | CCNGG | 7 cut(s) | 282, 526, 733, 845, 935, 955, 956 |
| BmsI | GCATC | 1 cut(s) | 649 |
| BmtI | GCTAGC | 1 cut(s) | 99 |
| BoxI | GACNNNNGTC | 1 cut(s) | 560 |
| Bpu10I | CCTNAGC | 1 cut(s) | 971 |
| BpuMI | CCSGG | 7 cut(s) | 282, 526, 733, 845, 935, 955, 956 |
| BsaAI | YACGTR | 1 cut(s) | 236 |
| BsaHI | GRCGYC | 1 cut(s) | 620 |
| BsaI | GGTCTC | 2 cut(s) | 330, 659 |
| BsaJI | CCNNGG | 9 cut(s) | 199, 245, 281, 327, 428, 565, 711, 953, 954 |
| BsaWI | WCCGGW | 2 cut(s) | 19, 876 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 30, 60 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 111, 284, 360, 401, 429, 434, 713, 799 |
| Bse118I | RCCGGY | 4 cut(s) | 227, 486, 727, 876 |
| BseAI | TCCGGA | 1 cut(s) | 19 |
| BseDI | CCNNGG | 9 cut(s) | 199, 245, 281, 327, 428, 565, 711, 953, 954 |
| BseGI | GGATG | 2 cut(s) | 49, 365 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 111, 284, 360, 401, 429, 434, 713, 799 |
| BseRI | GAGGAG | 4 cut(s) | 83, 659, 863, 992 |
| BseSI | GKGCMC | 1 cut(s) | 696 |
| BseX3I | CGGCCG | 1 cut(s) | 729 |
| BseXI | GCAGC | 3 cut(s) | 37, 265, 906 |
| BsgI | GTGCAG | 1 cut(s) | 272 |
| Bsh1236I | CGCG | 4 cut(s) | 450, 559, 582, 777 |
| Bsh1285I | CGRYCG | 2 cut(s) | 732, 753 |
| BshFI | GGCC | 7 cut(s) | 76, 288, 731, 843, 896, 959, 990 |
| BshTI | ACCGGT | 1 cut(s) | 876 |
| BsiEI | CGRYCG | 2 cut(s) | 732, 753 |
| BsiHKCI | CYCGRG | 3 cut(s) | 710, 830, 954 |
| BslFI | GGGAC | 2 cut(s) | 607, 661 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 111, 284, 360, 401, 429, 434, 713, 799 |
| BsmAI | GTCTC | 2 cut(s) | 330, 659 |
| BsmFI | GGGAC | 2 cut(s) | 607, 661 |
| BsmI | GAATGC | 1 cut(s) | 272 |
| BsnI | GGCC | 7 cut(s) | 76, 288, 731, 843, 896, 959, 990 |
| Bso31I | GGTCTC | 2 cut(s) | 330, 659 |
| BsoBI | CYCGRG | 3 cut(s) | 710, 830, 954 |
| Bsp1286I | GDGCHC | 1 cut(s) | 696 |
| Bsp13I | TCCGGA | 1 cut(s) | 19 |
| Bsp143I | GATC | 4 cut(s) | 474, 508, 591, 981 |
| BspANI | GGCC | 7 cut(s) | 76, 288, 731, 843, 896, 959, 990 |
| BspEI | TCCGGA | 1 cut(s) | 19 |
| BspFNI | CGCG | 4 cut(s) | 450, 559, 582, 777 |
| BspLI | GGNNCC | 6 cut(s) | 287, 476, 593, 676, 677, 705 |
| BspOI | GCTAGC | 1 cut(s) | 99 |
| BspPI | GGATC | 4 cut(s) | 469, 482, 586, 599 |
| BspTNI | GGTCTC | 2 cut(s) | 330, 659 |
| BsrBI | CCGCTC | 2 cut(s) | 749, 964 |
| BsrFI | RCCGGY | 4 cut(s) | 227, 486, 727, 876 |
| BssAI | RCCGGY | 4 cut(s) | 227, 486, 727, 876 |
| BssECI | CCNNGG | 9 cut(s) | 199, 245, 281, 327, 428, 565, 711, 953, 954 |
| BssMI | GATC | 4 cut(s) | 474, 508, 591, 981 |
| BssNI | GRCGYC | 1 cut(s) | 620 |
| BssT1I | CCWWGG | 3 cut(s) | 245, 327, 565 |
| Bst4CI | ACNGT | 4 cut(s) | 168, 200, 497, 552 |
| Bst6I | CTCTTC | 1 cut(s) | 682 |
| BstACI | GRCGYC | 1 cut(s) | 620 |
| BstBAI | YACGTR | 1 cut(s) | 236 |
| BstC8I | GCNNGC | 8 cut(s) | 97, 258, 488, 580, 586, 729, 743, 747 |
| BstDEI | CTNAG | 2 cut(s) | 151, 971 |
| BstDSI | CCRYGG | 1 cut(s) | 199 |
| BstEII | GGTNACC | 1 cut(s) | 879 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 427 |
| BstF5I | GGATG | 2 cut(s) | 49, 365 |
| BstFNI | CGCG | 4 cut(s) | 450, 559, 582, 777 |
| BstH2I | RGCGCY | 2 cut(s) | 93, 245 |
| BstHHI | GCGC | 3 cut(s) | 92, 244, 584 |
| BstKTI | GATC | 4 cut(s) | 477, 511, 594, 984 |
| BstMAI | GTCTC | 2 cut(s) | 330, 659 |
| BstMBI | GATC | 4 cut(s) | 474, 508, 591, 981 |
| BstMCI | CGRYCG | 2 cut(s) | 732, 753 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 96, 250, 659, 774 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 560 |
| BstPI | GGTNACC | 1 cut(s) | 879 |
| BstSCI | CCNGG | 7 cut(s) | 280, 524, 731, 843, 933, 953, 954 |
| BstSLI | GKGCMC | 1 cut(s) | 696 |
| BstUI | CGCG | 4 cut(s) | 450, 559, 582, 777 |
| BstV1I | GCAGC | 3 cut(s) | 37, 265, 906 |
| BstX2I | RGATCY | 3 cut(s) | 474, 508, 591 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 602 |
| BstYI | RGATCY | 3 cut(s) | 474, 508, 591 |
| BstZI | CGGCCG | 1 cut(s) | 729 |
| BsuRI | GGCC | 7 cut(s) | 76, 288, 731, 843, 896, 959, 990 |
| BtgI | CCRYGG | 1 cut(s) | 199 |
| BtsCI | GGATG | 2 cut(s) | 49, 365 |
| Cac8I | GCNNGC | 8 cut(s) | 97, 258, 488, 580, 586, 729, 743, 747 |
| CfoI | GCGC | 3 cut(s) | 92, 244, 584 |
| Cfr10I | RCCGGY | 4 cut(s) | 227, 486, 727, 876 |
| Cfr13I | GGNCC | 8 cut(s) | 184, 192, 209, 286, 675, 842, 957, 988 |
| Cfr9I | CCCGGG | 1 cut(s) | 954 |
| CseI | GACGC | 2 cut(s) | 565, 609 |
| Csp6I | GTAC | 2 cut(s) | 233, 606 |
| CspAI | ACCGGT | 1 cut(s) | 876 |
| CviAII | CATG | 2 cut(s) | 320, 923 |
| CviQI | GTAC | 2 cut(s) | 233, 606 |
| DdeI | CTNAG | 2 cut(s) | 151, 971 |
| DpnI | GATC | 4 cut(s) | 476, 510, 593, 983 |
| DpnII | GATC | 4 cut(s) | 474, 508, 591, 981 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 673 |
| DriI | GACNNNNNGTC | 1 cut(s) | 190 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 673 |
| EaeI | YGGCCR | 1 cut(s) | 729 |
| EagI | CGGCCG | 1 cut(s) | 729 |
| Eam1104I | CTCTTC | 1 cut(s) | 682 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 190 |
| EarI | CTCTTC | 1 cut(s) | 682 |
| EciI | GGCGGA | 4 cut(s) | 94, 219, 629, 696 |
| EclXI | CGGCCG | 1 cut(s) | 729 |
| Eco130I | CCWWGG | 3 cut(s) | 245, 327, 565 |
| Eco31I | GGTCTC | 2 cut(s) | 330, 659 |
| Eco47I | GGWCC | 4 cut(s) | 184, 192, 209, 675 |
| Eco52I | CGGCCG | 1 cut(s) | 729 |
| Eco88I | CYCGRG | 3 cut(s) | 710, 830, 954 |
| Eco91I | GGTNACC | 1 cut(s) | 879 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 427 |
| EcoO109I | RGGNCCY | 4 cut(s) | 209, 286, 675, 988 |
| EcoO65I | GGTNACC | 1 cut(s) | 879 |
| EcoRI | GAATTC | 1 cut(s) | 414 |
| EcoT14I | CCWWGG | 3 cut(s) | 245, 327, 565 |
| ErhI | CCWWGG | 3 cut(s) | 245, 327, 565 |
| FaeI | CATG | 2 cut(s) | 323, 926 |
| FaiI | YATR | 5 cut(s) | 215, 321, 440, 795, 924 |
| FaqI | GGGAC | 2 cut(s) | 607, 661 |
| FatI | CATG | 2 cut(s) | 319, 922 |
| FauI | CCCGC | 7 cut(s) | 57, 171, 249, 325, 367, 792, 957 |
| Fnu4HI | GCNGC | 5 cut(s) | 51, 93, 254, 769, 920 |
| FokI | GGATG | 2 cut(s) | 56, 372 |
| Fsp4HI | GCNGC | 5 cut(s) | 51, 93, 254, 769, 920 |
| FspBI | CTAG | 4 cut(s) | 30, 96, 506, 994 |
| GlaI | GCGC | 3 cut(s) | 91, 243, 583 |
| GluI | GCNGC | 5 cut(s) | 51, 93, 254, 769, 920 |
| HaeII | RGCGCY | 2 cut(s) | 93, 245 |
| HaeIII | GGCC | 7 cut(s) | 76, 288, 731, 843, 896, 959, 990 |
| HgaI | GACGC | 2 cut(s) | 565, 609 |
| HhaI | GCGC | 3 cut(s) | 92, 244, 584 |
| Hin1I | GRCGYC | 1 cut(s) | 620 |
| Hin1II | CATG | 2 cut(s) | 323, 926 |
| Hin6I | GCGC | 3 cut(s) | 90, 242, 582 |
| HinP1I | GCGC | 3 cut(s) | 90, 242, 582 |
| HincII | GTYRAC | 4 cut(s) | 171, 544, 555, 874 |
| HindII | GTYRAC | 4 cut(s) | 171, 544, 555, 874 |
| HinfI | GANTC | 6 cut(s) | 136, 272, 315, 561, 632, 759 |
| HphI | GGTGA | 4 cut(s) | 127, 267, 316, 873 |
| Hpy166II | GTNNAC | 6 cut(s) | 171, 233, 544, 555, 758, 874 |
| Hpy188I | TCNGA | 3 cut(s) | 404, 429, 637 |
| Hpy188III | TCNNGA | 3 cut(s) | 20, 302, 784 |
| Hpy8I | GTNNAC | 6 cut(s) | 171, 233, 544, 555, 758, 874 |
| Hpy99I | CGWCG | 4 cut(s) | 160, 168, 701, 946 |
| HpyAV | CCTTC | 6 cut(s) | 29, 299, 305, 350, 409, 895 |
| HpyCH4III | ACNGT | 4 cut(s) | 168, 200, 497, 552 |
| HpyCH4IV | ACGT | 1 cut(s) | 235 |
| HpyCH4V | TGCA | 3 cut(s) | 253, 741, 922 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 96, 250, 659, 774 |
| HpyF3I | CTNAG | 2 cut(s) | 151, 971 |
| HpySE526I | ACGT | 1 cut(s) | 235 |
| Hsp92I | GRCGYC | 1 cut(s) | 620 |
| Hsp92II | CATG | 2 cut(s) | 323, 926 |
| HspAI | GCGC | 3 cut(s) | 90, 242, 582 |
| KflI | GGGWCCC | 1 cut(s) | 675 |
| Kpn2I | TCCGGA | 1 cut(s) | 19 |
| KroI | GCCGGC | 2 cut(s) | 486, 727 |
| KroNI | GCCGGC | 2 cut(s) | 488, 729 |
| Kzo9I | GATC | 4 cut(s) | 474, 508, 591, 981 |
| LmnI | GCTCC | 3 cut(s) | 532, 709, 967 |
| Lsp1109I | GCAGC | 3 cut(s) | 37, 265, 906 |
| LweI | GCATC | 1 cut(s) | 649 |
| MaeI | CTAG | 4 cut(s) | 30, 96, 506, 994 |
| MaeII | ACGT | 1 cut(s) | 235 |
| MaeIII | GTNAC | 4 cut(s) | 273, 322, 497, 879 |
| MalI | GATC | 4 cut(s) | 476, 510, 593, 983 |
| MbiI | CCGCTC | 2 cut(s) | 749, 964 |
| MboI | GATC | 4 cut(s) | 474, 508, 591, 981 |
| MboII | GAAGA | 2 cut(s) | 464, 699 |
| MflI | RGATCY | 3 cut(s) | 474, 508, 591 |
| MhlI | GDGCHC | 1 cut(s) | 696 |
| MluCI | AATT | 4 cut(s) | 305, 414, 803, 926 |
| MlyI | GAGTC | 5 cut(s) | 145, 281, 570, 626, 753 |
| MmeI | TCCRAC | 3 cut(s) | 219, 443, 706 |
| MroI | TCCGGA | 1 cut(s) | 19 |
| MroNI | GCCGGC | 2 cut(s) | 486, 727 |
| MseI | TTAA | 2 cut(s) | 407, 863 |
| MslI | CAYNNNNRTG | 1 cut(s) | 600 |
| MspA1I | CMGCKG | 1 cut(s) | 256 |
| MspR9I | CCNGG | 7 cut(s) | 282, 526, 733, 845, 935, 955, 956 |
| Mva1269I | GAATGC | 1 cut(s) | 272 |
| MvnI | CGCG | 4 cut(s) | 450, 559, 582, 777 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 96, 250, 659, 774 |
| NaeI | GCCGGC | 2 cut(s) | 488, 729 |
| NciI | CCSGG | 7 cut(s) | 282, 526, 733, 845, 935, 955, 956 |
| NdeII | GATC | 4 cut(s) | 474, 508, 591, 981 |
| NgoMIV | GCCGGC | 2 cut(s) | 486, 727 |
| NheI | GCTAGC | 1 cut(s) | 95 |
| NlaIII | CATG | 2 cut(s) | 323, 926 |
| NlaIV | GGNNCC | 6 cut(s) | 287, 476, 593, 676, 677, 705 |
| NmeAIII | GCCGAG | 1 cut(s) | 985 |
| NmuCI | GTSAC | 3 cut(s) | 273, 322, 879 |
| OliI | CACNNNNGTG | 1 cut(s) | 600 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 556, 759 |
| PctI | GAATGC | 1 cut(s) | 272 |
| PdiI | GCCGGC | 2 cut(s) | 488, 729 |
| PfeI | GAWTC | 1 cut(s) | 315 |
| PfoI | TCCNGGA | 1 cut(s) | 933 |
| PinAI | ACCGGT | 1 cut(s) | 876 |
| PkrI | GCNGC | 5 cut(s) | 52, 94, 255, 770, 921 |
| PleI | GAGTC | 5 cut(s) | 144, 280, 569, 626, 753 |
| PpsI | GAGTC | 5 cut(s) | 144, 280, 569, 626, 753 |
| Ppu21I | YACGTR | 1 cut(s) | 236 |
| PpuMI | RGGWCCY | 2 cut(s) | 209, 675 |
| PshAI | GACNNNNGTC | 1 cut(s) | 560 |
| Psp5II | RGGWCCY | 2 cut(s) | 209, 675 |
| PspEI | GGTNACC | 1 cut(s) | 879 |
| PspN4I | GGNNCC | 6 cut(s) | 287, 476, 593, 676, 677, 705 |
| PspPI | GGNCC | 8 cut(s) | 184, 192, 209, 286, 675, 842, 957, 988 |
| PspPPI | RGGWCCY | 2 cut(s) | 209, 675 |
| PsuI | RGATCY | 3 cut(s) | 474, 508, 591 |
| RsaI | GTAC | 2 cut(s) | 234, 607 |
| RsaNI | GTAC | 2 cut(s) | 233, 606 |
| RseI | CAYNNNNRTG | 1 cut(s) | 600 |
| SaqAI | TTAA | 2 cut(s) | 407, 863 |
| SatI | GCNGC | 5 cut(s) | 51, 93, 254, 769, 920 |
| Sau3AI | GATC | 4 cut(s) | 474, 508, 591, 981 |
| Sau96I | GGNCC | 8 cut(s) | 184, 192, 209, 286, 675, 842, 957, 988 |
| SchI | GAGTC | 5 cut(s) | 145, 281, 570, 626, 753 |
| ScrFI | CCNGG | 7 cut(s) | 282, 526, 733, 845, 935, 955, 956 |
| SduI | GDGCHC | 1 cut(s) | 696 |
| SfaNI | GCATC | 1 cut(s) | 649 |
| SinI | GGWCC | 4 cut(s) | 184, 192, 209, 675 |
| SmaI | CCCGGG | 1 cut(s) | 956 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 600 |
| Sse9I | AATT | 4 cut(s) | 305, 414, 803, 926 |
| SspMI | CTAG | 4 cut(s) | 30, 96, 506, 994 |
| StyD4I | CCNGG | 7 cut(s) | 280, 524, 731, 843, 933, 953, 954 |
| StyI | CCWWGG | 3 cut(s) | 245, 327, 565 |
| TaaI | ACNGT | 4 cut(s) | 168, 200, 497, 552 |
| TaiI | ACGT | 1 cut(s) | 238 |
| TaqI | TCGA | 3 cut(s) | 301, 412, 573 |
| TaqII | GACCGA | 1 cut(s) | 201 |
| TasI | AATT | 4 cut(s) | 305, 414, 803, 926 |
| TauI | GCSGC | 2 cut(s) | 95, 771 |
| TfiI | GAWTC | 1 cut(s) | 315 |
| Tru1I | TTAA | 2 cut(s) | 407, 863 |
| Tru9I | TTAA | 2 cut(s) | 407, 863 |
| TseFI | GTSAC | 3 cut(s) | 273, 322, 879 |
| TseI | GCWGC | 3 cut(s) | 50, 253, 919 |
| Tsp45I | GTSAC | 3 cut(s) | 273, 322, 879 |
| TspDTI | ATGAA | 1 cut(s) | 939 |
| TspMI | CCCGGG | 1 cut(s) | 954 |
| VpaK11BI | GGWCC | 4 cut(s) | 184, 192, 209, 675 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 427 |
| XapI | RAATTY | 3 cut(s) | 305, 414, 803 |
| XmaI | CCCGGG | 1 cut(s) | 954 |
| XspI | CTAG | 4 cut(s) | 30, 96, 506, 994 |