Cytosolic purine
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 696 |
| Acc36I | ACCTGC | 3 cut(s) | 622, 674, 768 |
| Acc65I | GGTACC | 1 cut(s) | 1099 |
| AccB1I | GGYRCC | 4 cut(s) | 612, 998, 1099, 1440 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 463 |
| AccBSI | CCGCTC | 4 cut(s) | 27, 1363, 1396, 1438 |
| AccI | GTMKAC | 1 cut(s) | 775 |
| AccII | CGCG | 6 cut(s) | 706, 723, 858, 1205, 1292, 1479 |
| AclWI | GGATC | 5 cut(s) | 454, 920, 1195, 1203, 1216 |
| AcoI | YGGCCR | 3 cut(s) | 573, 726, 799 |
| AcsI | RAATTY | 3 cut(s) | 473, 639, 732 |
| AcuI | CTGAAG | 1 cut(s) | 380 |
| AcyI | GRCGYC | 5 cut(s) | 374, 613, 1237, 1340, 1441 |
| AfaI | GTAC | 3 cut(s) | 75, 890, 1101 |
| AfeI | AGCGCT | 1 cut(s) | 1170 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 78, 201, 388, 463, 654, 955, 1110, 1184, 1419 |
| AflIII | ACRYGT | 2 cut(s) | 507, 704 |
| AgsI | TTSAA | 1 cut(s) | 1229 |
| AjnI | CCWGG | 5 cut(s) | 239, 331, 423, 462, 876 |
| AleI | CACNNNNGTG | 1 cut(s) | 996 |
| AluBI | AGCT | 4 cut(s) | 346, 1369, 1402, 1486 |
| AluI | AGCT | 4 cut(s) | 346, 1369, 1402, 1486 |
| Alw21I | GWGCWC | 2 cut(s) | 408, 963 |
| Alw26I | GTCTC | 1 cut(s) | 875 |
| Alw44I | GTGCAC | 1 cut(s) | 404 |
| AlwI | GGATC | 5 cut(s) | 454, 920, 1195, 1203, 1216 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 463 |
| Ama87I | CYCGRG | 3 cut(s) | 22, 120, 1353 |
| Aor51HI | AGCGCT | 1 cut(s) | 1170 |
| ApaLI | GTGCAC | 1 cut(s) | 404 |
| ApoI | RAATTY | 3 cut(s) | 473, 639, 732 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1222, 1254 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 837 |
| Asp718I | GGTACC | 1 cut(s) | 1099 |
| AspLEI | GCGC | 8 cut(s) | 529, 615, 860, 1171, 1292, 1443, 1497, 1510 |
| AspS9I | GGNCC | 5 cut(s) | 281, 563, 853, 1029, 1118 |
| AsuC2I | CCSGG | 1 cut(s) | 276 |
| AsuHPI | GGTGA | 6 cut(s) | 27, 217, 263, 983, 1150, 1501 |
| AsuNHI | GCTAGC | 2 cut(s) | 185, 901 |
| AvaI | CYCGRG | 3 cut(s) | 22, 120, 1353 |
| AvaII | GGWCC | 3 cut(s) | 563, 853, 1029 |
| BaeGI | GKGCMC | 1 cut(s) | 408 |
| BalI | TGGCCA | 1 cut(s) | 728 |
| BamHI | GGATCC | 1 cut(s) | 1208 |
| BanI | GGYRCC | 4 cut(s) | 612, 998, 1099, 1440 |
| BauI | CACGAG | 3 cut(s) | 407, 962, 1322 |
| BbsI | GAAGAC | 1 cut(s) | 920 |
| Bbv12I | GWGCWC | 2 cut(s) | 408, 963 |
| BbvCI | CCTCAGC | 1 cut(s) | 1020 |
| BccI | CCATC | 3 cut(s) | 248, 283, 517 |
| BceAI | ACGGC | 4 cut(s) | 179, 386, 813, 1267 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 922, 956 |
| BciT130I | CCWGG | 5 cut(s) | 241, 333, 425, 464, 878 |
| BcnI | CCSGG | 1 cut(s) | 276 |
| BcoDI | GTCTC | 1 cut(s) | 875 |
| BfaI | CTAG | 4 cut(s) | 6, 186, 764, 902 |
| BfmI | CTRYAG | 1 cut(s) | 65 |
| BfoI | RGCGCY | 4 cut(s) | 530, 616, 1172, 1444 |
| BfuAI | ACCTGC | 3 cut(s) | 622, 674, 768 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 280, 391 |
| BlpI | GCTNAGC | 1 cut(s) | 944 |
| Bme1390I | CCNGG | 6 cut(s) | 241, 276, 333, 425, 464, 878 |
| Bme18I | GGWCC | 3 cut(s) | 563, 853, 1029 |
| BmeT110I | CYCGRG | 3 cut(s) | 22, 120, 1353 |
| BmgT120I | GGNCC | 5 cut(s) | 281, 563, 853, 1029, 1118 |
| BmrFI | CCNGG | 6 cut(s) | 241, 276, 333, 425, 464, 878 |
| BmrI | ACTGGG | 1 cut(s) | 1089 |
| BmsI | GCATC | 4 cut(s) | 124, 1264, 1312, 1482 |
| BmtI | GCTAGC | 2 cut(s) | 189, 905 |
| BmuI | ACTGGG | 1 cut(s) | 1089 |
| BoxI | GACNNNNGTC | 1 cut(s) | 771 |
| BpiI | GAAGAC | 1 cut(s) | 920 |
| BpmI | CTGGAG | 3 cut(s) | 440, 899, 1193 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1020, 1370 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 944 |
| BpuEI | CTTGAG | 2 cut(s) | 764, 1412 |
| BpuMI | CCSGG | 1 cut(s) | 276 |
| Bsa29I | ATCGAT | 1 cut(s) | 867 |
| BsaHI | GRCGYC | 5 cut(s) | 374, 613, 1237, 1340, 1441 |
| BsaI | GGTCTC | 1 cut(s) | 875 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 872, 902 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 78, 201, 388, 463, 654, 955, 1110, 1184, 1419 |
| Bse118I | RCCGGY | 4 cut(s) | 1031, 1052, 1110, 1144 |
| Bse1I | ACTGG | 4 cut(s) | 220, 949, 1084, 1338 |
| Bse3DI | GCAATG | 1 cut(s) | 1161 |
| BseBI | CCWGG | 5 cut(s) | 241, 333, 425, 464, 878 |
| BseCI | ATCGAT | 1 cut(s) | 867 |
| BseGI | GGATG | 1 cut(s) | 370 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 78, 201, 388, 463, 654, 955, 1110, 1184, 1419 |
| BseMI | GCAATG | 1 cut(s) | 1161 |
| BseMII | CTCAG | 6 cut(s) | 102, 644, 923, 935, 945, 1011 |
| BseNI | ACTGG | 4 cut(s) | 220, 949, 1084, 1338 |
| BseRI | GAGGAG | 2 cut(s) | 1277, 1391 |
| BseSI | GKGCMC | 1 cut(s) | 408 |
| BseYI | CCCAGC | 1 cut(s) | 1402 |
| Bsh1236I | CGCG | 6 cut(s) | 706, 723, 858, 1205, 1292, 1479 |
| Bsh1285I | CGRYCG | 1 cut(s) | 353 |
| BshNI | GGYRCC | 4 cut(s) | 612, 998, 1099, 1440 |
| BshVI | ATCGAT | 1 cut(s) | 867 |
| BsiEI | CGRYCG | 1 cut(s) | 353 |
| BsiHKAI | GWGCWC | 2 cut(s) | 408, 963 |
| BsiHKCI | CYCGRG | 3 cut(s) | 22, 120, 1353 |
| BsiSI | CCGG | 6 cut(s) | 275, 552, 1032, 1053, 1111, 1145 |
| BslFI | GGGAC | 3 cut(s) | 271, 1042, 1283 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 78, 201, 388, 463, 654, 955, 1110, 1184, 1419 |
| BsmAI | GTCTC | 1 cut(s) | 875 |
| BsmFI | GGGAC | 3 cut(s) | 271, 1042, 1283 |
| Bso31I | GGTCTC | 1 cut(s) | 875 |
| BsoBI | CYCGRG | 3 cut(s) | 22, 120, 1353 |
| Bsp1286I | GDGCHC | 2 cut(s) | 408, 963 |
| Bsp143I | GATC | 8 cut(s) | 414, 459, 803, 864, 925, 1187, 1208, 1380 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 944 |
| Bsp19I | CCATGG | 1 cut(s) | 90 |
| BspCNI | CTCAG | 6 cut(s) | 103, 645, 922, 936, 946, 1012 |
| BspDI | ATCGAT | 1 cut(s) | 867 |
| BspFNI | CGCG | 6 cut(s) | 706, 723, 858, 1205, 1292, 1479 |
| BspMI | ACCTGC | 3 cut(s) | 622, 674, 768 |
| BspOI | GCTAGC | 2 cut(s) | 189, 905 |
| BspPI | GGATC | 5 cut(s) | 454, 920, 1195, 1203, 1216 |
| BspT107I | GGYRCC | 4 cut(s) | 612, 998, 1099, 1440 |
| BspTNI | GGTCTC | 1 cut(s) | 875 |
| BsrBI | CCGCTC | 4 cut(s) | 27, 1363, 1396, 1438 |
| BsrDI | GCAATG | 1 cut(s) | 1161 |
| BsrFI | RCCGGY | 4 cut(s) | 1031, 1052, 1110, 1144 |
| BsrI | ACTGG | 4 cut(s) | 220, 949, 1084, 1338 |
| BssAI | RCCGGY | 4 cut(s) | 1031, 1052, 1110, 1144 |
| BssMI | GATC | 8 cut(s) | 414, 459, 803, 864, 925, 1187, 1208, 1380 |
| BssNI | GRCGYC | 5 cut(s) | 374, 613, 1237, 1340, 1441 |
| BssSI | CACGAG | 3 cut(s) | 407, 962, 1322 |
| BssT1I | CCWWGG | 4 cut(s) | 77, 90, 616, 994 |
| Bst2BI | CACGAG | 3 cut(s) | 407, 962, 1322 |
| Bst2UI | CCWGG | 5 cut(s) | 241, 333, 425, 464, 878 |
| Bst4CI | ACNGT | 8 cut(s) | 39, 73, 308, 571, 811, 919, 1043, 1156 |
| Bst6I | CTCTTC | 3 cut(s) | 219, 708, 1313 |
| BstACI | GRCGYC | 5 cut(s) | 374, 613, 1237, 1340, 1441 |
| BstDEI | CTNAG | 7 cut(s) | 111, 653, 909, 944, 954, 1020, 1370 |
| BstDSI | CCRYGG | 4 cut(s) | 90, 570, 1042, 1477 |
| BstEII | GGTNACC | 1 cut(s) | 1156 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 652 |
| BstF5I | GGATG | 1 cut(s) | 370 |
| BstFNI | CGCG | 6 cut(s) | 706, 723, 858, 1205, 1292, 1479 |
| BstH2I | RGCGCY | 4 cut(s) | 530, 616, 1172, 1444 |
| BstHHI | GCGC | 8 cut(s) | 529, 615, 860, 1171, 1292, 1443, 1497, 1510 |
| BstKTI | GATC | 8 cut(s) | 417, 462, 806, 867, 928, 1190, 1211, 1383 |
| BstMAI | GTCTC | 1 cut(s) | 875 |
| BstMBI | GATC | 8 cut(s) | 414, 459, 803, 864, 925, 1187, 1208, 1380 |
| BstMCI | CGRYCG | 1 cut(s) | 353 |
| BstNI | CCWGG | 5 cut(s) | 241, 333, 425, 464, 878 |
| BstNSI | RCATGY | 2 cut(s) | 398, 511 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 771 |
| BstPI | GGTNACC | 1 cut(s) | 1156 |
| BstSCI | CCNGG | 6 cut(s) | 239, 274, 331, 423, 462, 876 |
| BstSFI | CTRYAG | 1 cut(s) | 65 |
| BstSLI | GKGCMC | 1 cut(s) | 408 |
| BstUI | CGCG | 6 cut(s) | 706, 723, 858, 1205, 1292, 1479 |
| BstV2I | GAAGAC | 1 cut(s) | 920 |
| BstX2I | RGATCY | 2 cut(s) | 459, 1208 |
| BstYI | RGATCY | 2 cut(s) | 459, 1208 |
| Bsu15I | ATCGAT | 1 cut(s) | 867 |
| BsuTUI | ATCGAT | 1 cut(s) | 867 |
| BtgI | CCRYGG | 4 cut(s) | 90, 570, 1042, 1477 |
| BtsCI | GGATG | 1 cut(s) | 370 |
| BtsI | GCAGTG | 1 cut(s) | 631 |
| BtsIMutI | CAGTG | 3 cut(s) | 631, 1035, 1077 |
| BveI | ACCTGC | 3 cut(s) | 622, 674, 768 |
| CaiI | CAGNNNCTG | 1 cut(s) | 463 |
| CfoI | GCGC | 8 cut(s) | 529, 615, 860, 1171, 1292, 1443, 1497, 1510 |
| Cfr10I | RCCGGY | 4 cut(s) | 1031, 1052, 1110, 1144 |
| Cfr13I | GGNCC | 5 cut(s) | 281, 563, 853, 1029, 1118 |
| Cfr42I | CCGCGG | 1 cut(s) | 1480 |
| ClaI | ATCGAT | 1 cut(s) | 867 |
| CseI | GACGC | 4 cut(s) | 363, 943, 1226, 1329 |
| Csp6I | GTAC | 3 cut(s) | 74, 889, 1100 |
| CviAII | CATG | 4 cut(s) | 91, 395, 508, 886 |
| CviQI | GTAC | 3 cut(s) | 74, 889, 1100 |
| DdeI | CTNAG | 7 cut(s) | 111, 653, 909, 944, 954, 1020, 1370 |
| DinI | GGCGCC | 2 cut(s) | 614, 1442 |
| DpnI | GATC | 8 cut(s) | 416, 461, 805, 866, 927, 1189, 1210, 1382 |
| DpnII | GATC | 8 cut(s) | 414, 459, 803, 864, 925, 1187, 1208, 1380 |
| EaeI | YGGCCR | 3 cut(s) | 573, 726, 799 |
| Eam1104I | CTCTTC | 3 cut(s) | 219, 708, 1313 |
| EarI | CTCTTC | 3 cut(s) | 219, 708, 1313 |
| EciI | GGCGGA | 4 cut(s) | 314, 410, 763, 1071 |
| Eco130I | CCWWGG | 4 cut(s) | 77, 90, 616, 994 |
| Eco31I | GGTCTC | 1 cut(s) | 875 |
| Eco32I | GATATC | 1 cut(s) | 19 |
| Eco47I | GGWCC | 3 cut(s) | 563, 853, 1029 |
| Eco47III | AGCGCT | 1 cut(s) | 1170 |
| Eco57I | CTGAAG | 1 cut(s) | 380 |
| Eco88I | CYCGRG | 3 cut(s) | 22, 120, 1353 |
| Eco91I | GGTNACC | 1 cut(s) | 1156 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 652 |
| EcoO65I | GGTNACC | 1 cut(s) | 1156 |
| EcoRI | GAATTC | 2 cut(s) | 639, 732 |
| EcoRII | CCWGG | 5 cut(s) | 239, 331, 423, 462, 876 |
| EcoRV | GATATC | 1 cut(s) | 19 |
| EcoT14I | CCWWGG | 4 cut(s) | 77, 90, 616, 994 |
| EgeI | GGCGCC | 2 cut(s) | 614, 1442 |
| EheI | GGCGCC | 2 cut(s) | 614, 1442 |
| ErhI | CCWWGG | 4 cut(s) | 77, 90, 616, 994 |
| FaeI | CATG | 4 cut(s) | 94, 398, 511, 889 |
| FaqI | GGGAC | 3 cut(s) | 271, 1042, 1283 |
| FatI | CATG | 4 cut(s) | 90, 394, 507, 885 |
| FblI | GTMKAC | 1 cut(s) | 775 |
| FokI | GGATG | 1 cut(s) | 377 |
| FspBI | CTAG | 4 cut(s) | 6, 186, 764, 902 |
| GlaI | GCGC | 8 cut(s) | 528, 614, 859, 1170, 1291, 1442, 1496, 1509 |
| GsaI | CCCAGC | 1 cut(s) | 1406 |
| GsuI | CTGGAG | 3 cut(s) | 440, 899, 1193 |
| HaeII | RGCGCY | 4 cut(s) | 530, 616, 1172, 1444 |
| HapII | CCGG | 6 cut(s) | 275, 552, 1032, 1053, 1111, 1145 |
| HgaI | GACGC | 4 cut(s) | 363, 943, 1226, 1329 |
| HhaI | GCGC | 8 cut(s) | 529, 615, 860, 1171, 1292, 1443, 1497, 1510 |
| Hin1I | GRCGYC | 5 cut(s) | 374, 613, 1237, 1340, 1441 |
| Hin1II | CATG | 4 cut(s) | 94, 398, 511, 889 |
| Hin6I | GCGC | 8 cut(s) | 527, 613, 858, 1169, 1290, 1441, 1495, 1508 |
| HinP1I | GCGC | 8 cut(s) | 527, 613, 858, 1169, 1290, 1441, 1495, 1508 |
| HincII | GTYRAC | 3 cut(s) | 268, 304, 814 |
| HindII | GTYRAC | 3 cut(s) | 268, 304, 814 |
| HinfI | GANTC | 2 cut(s) | 233, 1250 |
| HpaII | CCGG | 6 cut(s) | 275, 552, 1032, 1053, 1111, 1145 |
| HphI | GGTGA | 6 cut(s) | 27, 217, 263, 983, 1150, 1501 |
| Hpy166II | GTNNAC | 5 cut(s) | 268, 304, 406, 776, 814 |
| Hpy188I | TCNGA | 6 cut(s) | 43, 638, 654, 843, 912, 955 |
| Hpy188III | TCNNGA | 4 cut(s) | 22, 110, 457, 1391 |
| Hpy8I | GTNNAC | 5 cut(s) | 268, 304, 406, 776, 814 |
| Hpy99I | CGWCG | 6 cut(s) | 379, 923, 937, 1019, 1242, 1345 |
| HpyAV | CCTTC | 3 cut(s) | 355, 1120, 1177 |
| HpyCH4III | ACNGT | 8 cut(s) | 39, 73, 308, 571, 811, 919, 1043, 1156 |
| HpyCH4IV | ACGT | 1 cut(s) | 434 |
| HpyCH4V | TGCA | 3 cut(s) | 406, 1166, 1321 |
| HpyF3I | CTNAG | 7 cut(s) | 111, 653, 909, 944, 954, 1020, 1370 |
| HpySE526I | ACGT | 1 cut(s) | 434 |
| Hsp92I | GRCGYC | 5 cut(s) | 374, 613, 1237, 1340, 1441 |
| Hsp92II | CATG | 4 cut(s) | 94, 398, 511, 889 |
| HspAI | GCGC | 8 cut(s) | 527, 613, 858, 1169, 1290, 1441, 1495, 1508 |
| KasI | GGCGCC | 2 cut(s) | 612, 1440 |
| KpnI | GGTACC | 1 cut(s) | 1103 |
| KroI | GCCGGC | 2 cut(s) | 1052, 1144 |
| KroNI | GCCGGC | 2 cut(s) | 1054, 1146 |
| KspI | CCGCGG | 1 cut(s) | 1480 |
| Kzo9I | GATC | 8 cut(s) | 414, 459, 803, 864, 925, 1187, 1208, 1380 |
| LmnI | GCTCC | 5 cut(s) | 197, 1174, 1279, 1399, 1435 |
| LweI | GCATC | 4 cut(s) | 124, 1264, 1312, 1482 |
| MaeI | CTAG | 4 cut(s) | 6, 186, 764, 902 |
| MaeII | ACGT | 1 cut(s) | 434 |
| MaeIII | GTNAC | 3 cut(s) | 430, 1156, 1329 |
| MalI | GATC | 8 cut(s) | 416, 461, 805, 866, 927, 1189, 1210, 1382 |
| MbiI | CCGCTC | 4 cut(s) | 27, 1363, 1396, 1438 |
| MboI | GATC | 8 cut(s) | 414, 459, 803, 864, 925, 1187, 1208, 1380 |
| MboII | GAAGA | 6 cut(s) | 143, 236, 409, 725, 925, 1300 |
| MflI | RGATCY | 2 cut(s) | 459, 1208 |
| MhlI | GDGCHC | 2 cut(s) | 408, 963 |
| MlsI | TGGCCA | 1 cut(s) | 728 |
| MluCI | AATT | 4 cut(s) | 473, 639, 732, 1513 |
| MluI | ACGCGT | 1 cut(s) | 704 |
| MluNI | TGGCCA | 1 cut(s) | 728 |
| Mly113I | GGCGCC | 2 cut(s) | 613, 1441 |
| MlyI | GAGTC | 1 cut(s) | 242 |
| MmeI | TCCRAC | 3 cut(s) | 590, 616, 668 |
| Mox20I | TGGCCA | 1 cut(s) | 728 |
| MroNI | GCCGGC | 2 cut(s) | 1052, 1144 |
| MroXI | GAANNNNTTC | 1 cut(s) | 837 |
| MscI | TGGCCA | 1 cut(s) | 728 |
| MseI | TTAA | 3 cut(s) | 531, 599, 1517 |
| MslI | CAYNNNNRTG | 1 cut(s) | 996 |
| Msp20I | TGGCCA | 1 cut(s) | 728 |
| MspA1I | CMGCKG | 6 cut(s) | 140, 197, 358, 389, 674, 1479 |
| MspI | CCGG | 6 cut(s) | 275, 552, 1032, 1053, 1111, 1145 |
| MspR9I | CCNGG | 6 cut(s) | 241, 276, 333, 425, 464, 878 |
| MvaI | CCWGG | 5 cut(s) | 241, 333, 425, 464, 878 |
| MvnI | CGCG | 6 cut(s) | 706, 723, 858, 1205, 1292, 1479 |
| NaeI | GCCGGC | 2 cut(s) | 1054, 1146 |
| NarI | GGCGCC | 2 cut(s) | 613, 1441 |
| NciI | CCSGG | 1 cut(s) | 276 |
| NcoI | CCATGG | 1 cut(s) | 90 |
| NdeII | GATC | 8 cut(s) | 414, 459, 803, 864, 925, 1187, 1208, 1380 |
| NgoMIV | GCCGGC | 2 cut(s) | 1052, 1144 |
| NheI | GCTAGC | 2 cut(s) | 185, 901 |
| NlaIII | CATG | 4 cut(s) | 94, 398, 511, 889 |
| NmeAIII | GCCGAG | 2 cut(s) | 601, 886 |
| NmuCI | GTSAC | 2 cut(s) | 430, 1156 |
| NspI | RCATGY | 2 cut(s) | 398, 511 |
| OliI | CACNNNNGTG | 1 cut(s) | 996 |
| PaeI | GCATGC | 1 cut(s) | 398 |
| PaeR7I | CTCGAG | 1 cut(s) | 22 |
| PciI | ACATGT | 1 cut(s) | 507 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 103 |
| PdiI | GCCGGC | 2 cut(s) | 1054, 1146 |
| PdmI | GAANNNNTTC | 1 cut(s) | 837 |
| PfeI | GAWTC | 1 cut(s) | 1250 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 463 |
| PfoI | TCCNGGA | 1 cut(s) | 876 |
| PleI | GAGTC | 1 cut(s) | 241 |
| PluTI | GGCGCC | 2 cut(s) | 616, 1444 |
| PpsI | GAGTC | 1 cut(s) | 241 |
| PscI | ACATGT | 1 cut(s) | 507 |
| PshAI | GACNNNNGTC | 1 cut(s) | 771 |
| PsiI | TTATAA | 1 cut(s) | 696 |
| Psp6I | CCWGG | 5 cut(s) | 239, 331, 423, 462, 876 |
| PspEI | GGTNACC | 1 cut(s) | 1156 |
| PspFI | CCCAGC | 1 cut(s) | 1402 |
| PspGI | CCWGG | 5 cut(s) | 239, 331, 423, 462, 876 |
| PspPI | GGNCC | 5 cut(s) | 281, 563, 853, 1029, 1118 |
| PstNI | CAGNNNCTG | 1 cut(s) | 463 |
| PsuI | RGATCY | 2 cut(s) | 459, 1208 |
| RsaI | GTAC | 3 cut(s) | 75, 890, 1101 |
| RsaNI | GTAC | 3 cut(s) | 74, 889, 1100 |
| RseI | CAYNNNNRTG | 1 cut(s) | 996 |
| SacII | CCGCGG | 1 cut(s) | 1480 |
| SaqAI | TTAA | 3 cut(s) | 531, 599, 1517 |
| Sau3AI | GATC | 8 cut(s) | 414, 459, 803, 864, 925, 1187, 1208, 1380 |
| Sau96I | GGNCC | 5 cut(s) | 281, 563, 853, 1029, 1118 |
| SchI | GAGTC | 1 cut(s) | 242 |
| ScrFI | CCNGG | 6 cut(s) | 241, 276, 333, 425, 464, 878 |
| SduI | GDGCHC | 2 cut(s) | 408, 963 |
| SfaNI | GCATC | 4 cut(s) | 124, 1264, 1312, 1482 |
| SfcI | CTRYAG | 1 cut(s) | 65 |
| SfoI | GGCGCC | 2 cut(s) | 614, 1442 |
| Sfr274I | CTCGAG | 1 cut(s) | 22 |
| Sfr303I | CCGCGG | 1 cut(s) | 1480 |
| SgrBI | CCGCGG | 1 cut(s) | 1480 |
| SinI | GGWCC | 3 cut(s) | 563, 853, 1029 |
| SlaI | CTCGAG | 1 cut(s) | 22 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 996 |
| SmlI | CTYRAG | 3 cut(s) | 22, 779, 1391 |
| SmoI | CTYRAG | 3 cut(s) | 22, 779, 1391 |
| SphI | GCATGC | 1 cut(s) | 398 |
| Sse9I | AATT | 4 cut(s) | 473, 639, 732, 1513 |
| SspDI | GGCGCC | 2 cut(s) | 612, 1440 |
| SspMI | CTAG | 4 cut(s) | 6, 186, 764, 902 |
| StyD4I | CCNGG | 6 cut(s) | 239, 274, 331, 423, 462, 876 |
| StyI | CCWWGG | 4 cut(s) | 77, 90, 616, 994 |
| TaaI | ACNGT | 8 cut(s) | 39, 73, 308, 571, 811, 919, 1043, 1156 |
| TaiI | ACGT | 1 cut(s) | 437 |
| TaqI | TCGA | 5 cut(s) | 23, 736, 867, 1190, 1343 |
| TasI | AATT | 4 cut(s) | 473, 639, 732, 1513 |
| TfiI | GAWTC | 1 cut(s) | 1250 |
| Tru1I | TTAA | 3 cut(s) | 531, 599, 1517 |
| Tru9I | TTAA | 3 cut(s) | 531, 599, 1517 |
| TscAI | CASTG | 3 cut(s) | 631, 1042, 1084 |
| TseFI | GTSAC | 2 cut(s) | 430, 1156 |
| Tsp45I | GTSAC | 2 cut(s) | 430, 1156 |
| TspDTI | ATGAA | 2 cut(s) | 326, 381 |
| TspRI | CASTG | 3 cut(s) | 631, 1042, 1084 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 463 |
| VneI | GTGCAC | 1 cut(s) | 404 |
| VpaK11BI | GGWCC | 3 cut(s) | 563, 853, 1029 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 652 |
| XapI | RAATTY | 3 cut(s) | 473, 639, 732 |
| XceI | RCATGY | 2 cut(s) | 398, 511 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 623, 992, 1168, 1420 |
| XhoI | CTCGAG | 1 cut(s) | 22 |
| XmiI | GTMKAC | 1 cut(s) | 775 |
| XmnI | GAANNNNTTC | 1 cut(s) | 837 |
| XspI | CTAG | 4 cut(s) | 6, 186, 764, 902 |