No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1375 |
| Acc65I | GGTACC | 2 cut(s) | 457, 1499 |
| AccB1I | GGYRCC | 6 cut(s) | 73, 368, 457, 706, 1382, 1499 |
| AccBSI | CCGCTC | 2 cut(s) | 116, 1060 |
| AccI | GTMKAC | 2 cut(s) | 234, 883 |
| AclWI | GGATC | 3 cut(s) | 160, 173, 752 |
| AcoI | YGGCCR | 3 cut(s) | 408, 987, 1182 |
| AcuI | CTGAAG | 4 cut(s) | 387, 1185, 1298, 1593 |
| AcyI | GRCGYC | 4 cut(s) | 707, 934, 957, 966 |
| AfaI | GTAC | 5 cut(s) | 50, 459, 1220, 1365, 1501 |
| AflIII | ACRYGT | 2 cut(s) | 1018, 1357 |
| AgeI | ACCGGT | 1 cut(s) | 215 |
| AgsI | TTSAA | 3 cut(s) | 104, 260, 1204 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1515 |
| AjnI | CCWGG | 3 cut(s) | 51, 601, 1473 |
| AleI | CACNNNNGTG | 1 cut(s) | 471 |
| AluBI | AGCT | 9 cut(s) | 226, 241, 437, 674, 783, 862, 895, 979, 1084 |
| AluI | AGCT | 9 cut(s) | 226, 241, 437, 674, 783, 862, 895, 979, 1084 |
| Alw21I | GWGCWC | 3 cut(s) | 864, 1345, 1414 |
| Alw26I | GTCTC | 3 cut(s) | 1027, 1317, 1502 |
| AlwI | GGATC | 3 cut(s) | 160, 173, 752 |
| Ama87I | CYCGRG | 1 cut(s) | 1513 |
| ApaI | GGGCCC | 1 cut(s) | 1258 |
| ApeKI | GCWGC | 8 cut(s) | 241, 437, 499, 686, 811, 841, 1051, 1096 |
| AsiGI | ACCGGT | 1 cut(s) | 215 |
| Asp718I | GGTACC | 2 cut(s) | 457, 1499 |
| AspLEI | GCGC | 7 cut(s) | 141, 580, 709, 753, 1292, 1483, 1553 |
| AsuC2I | CCSGG | 4 cut(s) | 270, 1331, 1418, 1490 |
| AsuHPI | GGTGA | 2 cut(s) | 349, 1138 |
| AvaI | CYCGRG | 1 cut(s) | 1513 |
| AvaII | GGWCC | 4 cut(s) | 18, 310, 476, 646 |
| AxyI | CCTNAGG | 1 cut(s) | 1157 |
| BaeGI | GKGCMC | 3 cut(s) | 76, 1258, 1329 |
| BalI | TGGCCA | 1 cut(s) | 989 |
| BamHI | GGATCC | 1 cut(s) | 165 |
| BanI | GGYRCC | 6 cut(s) | 73, 368, 457, 706, 1382, 1499 |
| BanII | GRGCYC | 2 cut(s) | 864, 1258 |
| Bbv12I | GWGCWC | 3 cut(s) | 864, 1345, 1414 |
| BbvCI | CCTCAGC | 1 cut(s) | 1431 |
| BbvI | GCAGC | 8 cut(s) | 228, 424, 511, 673, 798, 853, 1038, 1108 |
| BccI | CCATC | 4 cut(s) | 369, 979, 998, 1460 |
| BceAI | ACGGC | 1 cut(s) | 947 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1542, 1576 |
| BciT130I | CCWGG | 3 cut(s) | 53, 603, 1475 |
| BcnI | CCSGG | 4 cut(s) | 270, 1331, 1418, 1490 |
| BcoDI | GTCTC | 3 cut(s) | 1027, 1317, 1502 |
| BfaI | CTAG | 2 cut(s) | 740, 1259 |
| BfmI | CTRYAG | 3 cut(s) | 778, 884, 1052 |
| BfoI | RGCGCY | 3 cut(s) | 581, 710, 1293 |
| BfuAI | ACCTGC | 1 cut(s) | 1375 |
| Bme1390I | CCNGG | 7 cut(s) | 53, 270, 603, 1331, 1418, 1475, 1490 |
| Bme18I | GGWCC | 4 cut(s) | 18, 310, 476, 646 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1515 |
| BmeT110I | CYCGRG | 1 cut(s) | 1513 |
| BmrFI | CCNGG | 7 cut(s) | 53, 270, 603, 1331, 1418, 1475, 1490 |
| BmrI | ACTGGG | 1 cut(s) | 528 |
| BmsI | GCATC | 2 cut(s) | 330, 336 |
| BmuI | ACTGGG | 1 cut(s) | 528 |
| BpmI | CTGGAG | 1 cut(s) | 1157 |
| Bpu10I | CCTNAGC | 3 cut(s) | 863, 1390, 1431 |
| BpuEI | CTTGAG | 1 cut(s) | 602 |
| BpuMI | CCSGG | 4 cut(s) | 270, 1331, 1418, 1490 |
| BsaBI | GATNNNNATC | 1 cut(s) | 989 |
| BsaHI | GRCGYC | 4 cut(s) | 707, 934, 957, 966 |
| BsaI | GGTCTC | 1 cut(s) | 1502 |
| BsaJI | CCNNGG | 9 cut(s) | 169, 324, 524, 572, 960, 970, 1329, 1374, 1528 |
| BsaWI | WCCGGW | 2 cut(s) | 215, 331 |
| Bse118I | RCCGGY | 4 cut(s) | 215, 410, 502, 538 |
| Bse1I | ACTGG | 5 cut(s) | 14, 201, 523, 769, 921 |
| Bse21I | CCTNAGG | 1 cut(s) | 1157 |
| Bse3DI | GCAATG | 1 cut(s) | 312 |
| Bse8I | GATNNNNATC | 1 cut(s) | 989 |
| BseBI | CCWGG | 3 cut(s) | 53, 603, 1475 |
| BseDI | CCNNGG | 9 cut(s) | 169, 324, 524, 572, 960, 970, 1329, 1374, 1528 |
| BseGI | GGATG | 2 cut(s) | 634, 990 |
| BseJI | GATNNNNATC | 1 cut(s) | 989 |
| BseMI | GCAATG | 1 cut(s) | 312 |
| BseMII | CTCAG | 5 cut(s) | 877, 1148, 1328, 1445, 1460 |
| BseNI | ACTGG | 5 cut(s) | 14, 201, 523, 769, 921 |
| BseRI | GAGGAG | 2 cut(s) | 545, 638 |
| BseSI | GKGCMC | 3 cut(s) | 76, 1258, 1329 |
| BseX3I | CGGCCG | 1 cut(s) | 1182 |
| BseXI | GCAGC | 8 cut(s) | 228, 424, 511, 673, 798, 853, 1038, 1108 |
| BseYI | CCCAGC | 3 cut(s) | 56, 433, 1250 |
| BsgI | GTGCAG | 2 cut(s) | 518, 1115 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1185 |
| BshNI | GGYRCC | 6 cut(s) | 73, 368, 457, 706, 1382, 1499 |
| BshTI | ACCGGT | 1 cut(s) | 215 |
| BsiEI | CGRYCG | 1 cut(s) | 1185 |
| BsiHKAI | GWGCWC | 3 cut(s) | 864, 1345, 1414 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1513 |
| BslFI | GGGAC | 5 cut(s) | 31, 132, 462, 1013, 1503 |
| BsmAI | GTCTC | 3 cut(s) | 1027, 1317, 1502 |
| BsmBI | CGTCTC | 2 cut(s) | 1027, 1317 |
| BsmFI | GGGAC | 5 cut(s) | 31, 132, 462, 1013, 1503 |
| BsmI | GAATGC | 1 cut(s) | 1199 |
| Bso31I | GGTCTC | 1 cut(s) | 1502 |
| BsoBI | CYCGRG | 1 cut(s) | 1513 |
| Bsp120I | GGGCCC | 1 cut(s) | 1254 |
| Bsp1286I | GDGCHC | 6 cut(s) | 76, 864, 1258, 1329, 1345, 1414 |
| Bsp143I | GATC | 3 cut(s) | 165, 744, 1537 |
| Bsp68I | TCGCGA | 1 cut(s) | 288 |
| BspCNI | CTCAG | 5 cut(s) | 876, 1149, 1327, 1444, 1459 |
| BspMAI | CTGCAG | 1 cut(s) | 1056 |
| BspMI | ACCTGC | 1 cut(s) | 1375 |
| BspPI | GGATC | 3 cut(s) | 160, 173, 752 |
| BspT107I | GGYRCC | 6 cut(s) | 73, 368, 457, 706, 1382, 1499 |
| BspTNI | GGTCTC | 1 cut(s) | 1502 |
| BsrBI | CCGCTC | 2 cut(s) | 116, 1060 |
| BsrDI | GCAATG | 1 cut(s) | 312 |
| BsrFI | RCCGGY | 4 cut(s) | 215, 410, 502, 538 |
| BsrI | ACTGG | 5 cut(s) | 14, 201, 523, 769, 921 |
| BssAI | RCCGGY | 4 cut(s) | 215, 410, 502, 538 |
| BssECI | CCNNGG | 9 cut(s) | 169, 324, 524, 572, 960, 970, 1329, 1374, 1528 |
| BssMI | GATC | 3 cut(s) | 165, 744, 1537 |
| BssNI | GRCGYC | 4 cut(s) | 707, 934, 957, 966 |
| BssT1I | CCWWGG | 3 cut(s) | 524, 572, 1528 |
| Bst2UI | CCWGG | 3 cut(s) | 53, 603, 1475 |
| Bst4CI | ACNGT | 7 cut(s) | 314, 377, 496, 514, 953, 1209, 1499 |
| Bst6I | CTCTTC | 3 cut(s) | 141, 609, 615 |
| BstACI | GRCGYC | 4 cut(s) | 707, 934, 957, 966 |
| BstDEI | CTNAG | 8 cut(s) | 863, 1071, 1157, 1314, 1335, 1390, 1431, 1446 |
| BstDSI | CCRYGG | 3 cut(s) | 960, 970, 1374 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1052 |
| BstF5I | GGATG | 2 cut(s) | 634, 990 |
| BstH2I | RGCGCY | 3 cut(s) | 581, 710, 1293 |
| BstHHI | GCGC | 7 cut(s) | 141, 580, 709, 753, 1292, 1483, 1553 |
| BstKTI | GATC | 3 cut(s) | 168, 747, 1540 |
| BstMAI | GTCTC | 3 cut(s) | 1027, 1317, 1502 |
| BstMBI | GATC | 3 cut(s) | 165, 744, 1537 |
| BstMCI | CGRYCG | 1 cut(s) | 1185 |
| BstNI | CCWGG | 3 cut(s) | 53, 603, 1475 |
| BstNSI | RCATGY | 1 cut(s) | 1571 |
| BstSCI | CCNGG | 7 cut(s) | 51, 268, 601, 1329, 1416, 1473, 1488 |
| BstSFI | CTRYAG | 3 cut(s) | 778, 884, 1052 |
| BstSLI | GKGCMC | 3 cut(s) | 76, 1258, 1329 |
| BstV1I | GCAGC | 8 cut(s) | 228, 424, 511, 673, 798, 853, 1038, 1108 |
| BstX2I | RGATCY | 2 cut(s) | 165, 744 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 473, 723 |
| BstYI | RGATCY | 2 cut(s) | 165, 744 |
| BstZI | CGGCCG | 1 cut(s) | 1182 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1157 |
| BtgI | CCRYGG | 3 cut(s) | 960, 970, 1374 |
| BtgZI | GCGATG | 1 cut(s) | 1027 |
| BtsCI | GGATG | 2 cut(s) | 634, 990 |
| BtsI | GCAGTG | 1 cut(s) | 1402 |
| BtsIMutI | CAGTG | 3 cut(s) | 411, 516, 1402 |
| BtuMI | TCGCGA | 1 cut(s) | 288 |
| BveI | ACCTGC | 1 cut(s) | 1375 |
| CciNI | GCGGCCGC | 1 cut(s) | 1182 |
| CfoI | GCGC | 7 cut(s) | 141, 580, 709, 753, 1292, 1483, 1553 |
| Cfr10I | RCCGGY | 4 cut(s) | 215, 410, 502, 538 |
| Cfr42I | CCGCGG | 3 cut(s) | 963, 973, 1377 |
| CseI | GACGC | 8 cut(s) | 16, 299, 581, 923, 965, 974, 1009, 1299 |
| Csp6I | GTAC | 5 cut(s) | 49, 458, 1219, 1364, 1500 |
| CspAI | ACCGGT | 1 cut(s) | 215 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 745, 780 |
| CviAII | CATG | 3 cut(s) | 815, 1461, 1568 |
| CviQI | GTAC | 5 cut(s) | 49, 458, 1219, 1364, 1500 |
| DdeI | CTNAG | 8 cut(s) | 863, 1071, 1157, 1314, 1335, 1390, 1431, 1446 |
| DinI | GGCGCC | 1 cut(s) | 708 |
| DpnI | GATC | 3 cut(s) | 167, 746, 1539 |
| DpnII | GATC | 3 cut(s) | 165, 744, 1537 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1515 |
| EaeI | YGGCCR | 3 cut(s) | 408, 987, 1182 |
| EagI | CGGCCG | 1 cut(s) | 1182 |
| Eam1104I | CTCTTC | 3 cut(s) | 141, 609, 615 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1515 |
| EarI | CTCTTC | 3 cut(s) | 141, 609, 615 |
| Ecl136II | GAGCTC | 1 cut(s) | 862 |
| EclXI | CGGCCG | 1 cut(s) | 1182 |
| Eco130I | CCWWGG | 3 cut(s) | 524, 572, 1528 |
| Eco24I | GRGCYC | 2 cut(s) | 864, 1258 |
| Eco31I | GGTCTC | 1 cut(s) | 1502 |
| Eco47I | GGWCC | 4 cut(s) | 18, 310, 476, 646 |
| Eco52I | CGGCCG | 1 cut(s) | 1182 |
| Eco53kI | GAGCTC | 1 cut(s) | 862 |
| Eco57I | CTGAAG | 4 cut(s) | 387, 1185, 1298, 1593 |
| Eco81I | CCTNAGG | 1 cut(s) | 1157 |
| Eco88I | CYCGRG | 1 cut(s) | 1513 |
| EcoICRI | GAGCTC | 1 cut(s) | 862 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1052 |
| EcoO109I | RGGNCCY | 6 cut(s) | 18, 599, 1228, 1254, 1255, 1427 |
| EcoRII | CCWGG | 3 cut(s) | 51, 601, 1473 |
| EcoT14I | CCWWGG | 3 cut(s) | 524, 572, 1528 |
| EcoT38I | GRGCYC | 2 cut(s) | 864, 1258 |
| EgeI | GGCGCC | 1 cut(s) | 708 |
| EheI | GGCGCC | 1 cut(s) | 708 |
| ErhI | CCWWGG | 3 cut(s) | 524, 572, 1528 |
| Esp3I | CGTCTC | 2 cut(s) | 1027, 1317 |
| FaeI | CATG | 3 cut(s) | 818, 1464, 1571 |
| FaiI | YATR | 8 cut(s) | 108, 129, 483, 816, 1462, 1505, 1523, 1569 |
| FaqI | GGGAC | 5 cut(s) | 31, 132, 462, 1013, 1503 |
| FatI | CATG | 3 cut(s) | 814, 1460, 1567 |
| FblI | GTMKAC | 2 cut(s) | 234, 883 |
| FokI | GGATG | 2 cut(s) | 641, 977 |
| FriOI | GRGCYC | 2 cut(s) | 864, 1258 |
| FspBI | CTAG | 2 cut(s) | 740, 1259 |
| GlaI | GCGC | 7 cut(s) | 140, 579, 708, 752, 1291, 1482, 1552 |
| GsaI | CCCAGC | 3 cut(s) | 60, 437, 1254 |
| GsuI | CTGGAG | 1 cut(s) | 1157 |
| HaeII | RGCGCY | 3 cut(s) | 581, 710, 1293 |
| HgaI | GACGC | 8 cut(s) | 16, 299, 581, 923, 965, 974, 1009, 1299 |
| HhaI | GCGC | 7 cut(s) | 141, 580, 709, 753, 1292, 1483, 1553 |
| Hin1I | GRCGYC | 4 cut(s) | 707, 934, 957, 966 |
| Hin1II | CATG | 3 cut(s) | 818, 1464, 1571 |
| Hin6I | GCGC | 7 cut(s) | 139, 578, 707, 751, 1290, 1481, 1551 |
| HinP1I | GCGC | 7 cut(s) | 139, 578, 707, 751, 1290, 1481, 1551 |
| HincII | GTYRAC | 1 cut(s) | 235 |
| HindII | GTYRAC | 1 cut(s) | 235 |
| HinfI | GANTC | 9 cut(s) | 184, 283, 320, 568, 727, 1103, 1238, 1438, 1516 |
| HphI | GGTGA | 2 cut(s) | 349, 1138 |
| Hpy166II | GTNNAC | 2 cut(s) | 235, 884 |
| Hpy188I | TCNGA | 8 cut(s) | 97, 113, 170, 213, 325, 1304, 1453, 1537 |
| Hpy188III | TCNNGA | 3 cut(s) | 287, 392, 1156 |
| Hpy8I | GTNNAC | 2 cut(s) | 235, 884 |
| Hpy99I | CGWCG | 8 cut(s) | 32, 82, 293, 377, 597, 763, 1181, 1564 |
| HpyCH4III | ACNGT | 7 cut(s) | 314, 377, 496, 514, 953, 1209, 1499 |
| HpyCH4IV | ACGT | 3 cut(s) | 80, 1176, 1357 |
| HpyCH4V | TGCA | 4 cut(s) | 499, 814, 1054, 1096 |
| HpyF3I | CTNAG | 8 cut(s) | 863, 1071, 1157, 1314, 1335, 1390, 1431, 1446 |
| HpySE526I | ACGT | 3 cut(s) | 80, 1176, 1357 |
| Hsp92I | GRCGYC | 4 cut(s) | 707, 934, 957, 966 |
| Hsp92II | CATG | 3 cut(s) | 818, 1464, 1571 |
| HspAI | GCGC | 7 cut(s) | 139, 578, 707, 751, 1290, 1481, 1551 |
| KasI | GGCGCC | 1 cut(s) | 706 |
| KpnI | GGTACC | 2 cut(s) | 461, 1503 |
| KroI | GCCGGC | 3 cut(s) | 410, 502, 538 |
| KroNI | GCCGGC | 3 cut(s) | 412, 504, 540 |
| KspI | CCGCGG | 3 cut(s) | 963, 973, 1377 |
| Kzo9I | GATC | 3 cut(s) | 165, 744, 1537 |
| Lsp1109I | GCAGC | 8 cut(s) | 228, 424, 511, 673, 798, 853, 1038, 1108 |
| LweI | GCATC | 2 cut(s) | 330, 336 |
| MaeI | CTAG | 2 cut(s) | 740, 1259 |
| MaeII | ACGT | 3 cut(s) | 80, 1176, 1357 |
| MaeIII | GTNAC | 3 cut(s) | 272, 514, 953 |
| MalI | GATC | 3 cut(s) | 167, 746, 1539 |
| MbiI | CCGCTC | 2 cut(s) | 116, 1060 |
| MboI | GATC | 3 cut(s) | 165, 744, 1537 |
| MboII | GAAGA | 6 cut(s) | 155, 158, 626, 632, 670, 1592 |
| MflI | RGATCY | 2 cut(s) | 165, 744 |
| MhlI | GDGCHC | 6 cut(s) | 76, 864, 1258, 1329, 1345, 1414 |
| MlsI | TGGCCA | 1 cut(s) | 989 |
| MluCI | AATT | 1 cut(s) | 87 |
| MluI | ACGCGT | 1 cut(s) | 1018 |
| MluNI | TGGCCA | 1 cut(s) | 989 |
| Mly113I | GGCGCC | 1 cut(s) | 707 |
| MlyI | GAGTC | 6 cut(s) | 292, 329, 1112, 1247, 1432, 1525 |
| MmeI | TCCRAC | 3 cut(s) | 75, 1011, 1431 |
| Mox20I | TGGCCA | 1 cut(s) | 989 |
| MreI | CGCCGGCG | 1 cut(s) | 538 |
| MroNI | GCCGGC | 3 cut(s) | 410, 502, 538 |
| MscI | TGGCCA | 1 cut(s) | 989 |
| MseI | TTAA | 1 cut(s) | 90 |
| MslI | CAYNNNNRTG | 3 cut(s) | 366, 471, 1091 |
| Msp20I | TGGCCA | 1 cut(s) | 989 |
| MspA1I | CMGCKG | 9 cut(s) | 241, 437, 560, 962, 972, 997, 1099, 1190, 1376 |
| MspR9I | CCNGG | 7 cut(s) | 53, 270, 603, 1331, 1418, 1475, 1490 |
| Mva1269I | GAATGC | 1 cut(s) | 1199 |
| MvaI | CCWGG | 3 cut(s) | 53, 603, 1475 |
| NaeI | GCCGGC | 3 cut(s) | 412, 504, 540 |
| NarI | GGCGCC | 1 cut(s) | 707 |
| NciI | CCSGG | 4 cut(s) | 270, 1331, 1418, 1490 |
| NdeII | GATC | 3 cut(s) | 165, 744, 1537 |
| NgoMIV | GCCGGC | 3 cut(s) | 410, 502, 538 |
| NlaIII | CATG | 3 cut(s) | 818, 1464, 1571 |
| NmeAIII | GCCGAG | 1 cut(s) | 764 |
| NmuCI | GTSAC | 2 cut(s) | 514, 953 |
| NotI | GCGGCCGC | 1 cut(s) | 1182 |
| NruI | TCGCGA | 1 cut(s) | 288 |
| NspI | RCATGY | 1 cut(s) | 1571 |
| OliI | CACNNNNGTG | 1 cut(s) | 471 |
| PaeI | GCATGC | 1 cut(s) | 1571 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 755 |
| PctI | GAATGC | 1 cut(s) | 1199 |
| PdiI | GCCGGC | 3 cut(s) | 412, 504, 540 |
| PfeI | GAWTC | 3 cut(s) | 184, 568, 727 |
| PflFI | GACNNNGTC | 1 cut(s) | 1237 |
| PinAI | ACCGGT | 1 cut(s) | 215 |
| PleI | GAGTC | 6 cut(s) | 291, 328, 1111, 1246, 1432, 1524 |
| PluTI | GGCGCC | 1 cut(s) | 710 |
| PpsI | GAGTC | 6 cut(s) | 291, 328, 1111, 1246, 1432, 1524 |
| PpuMI | RGGWCCY | 1 cut(s) | 18 |
| Psp124BI | GAGCTC | 1 cut(s) | 864 |
| Psp5II | RGGWCCY | 1 cut(s) | 18 |
| Psp6I | CCWGG | 3 cut(s) | 51, 601, 1473 |
| PspFI | CCCAGC | 3 cut(s) | 56, 433, 1250 |
| PspGI | CCWGG | 3 cut(s) | 51, 601, 1473 |
| PspOMI | GGGCCC | 1 cut(s) | 1254 |
| PspPPI | RGGWCCY | 1 cut(s) | 18 |
| PstI | CTGCAG | 1 cut(s) | 1056 |
| PsuI | RGATCY | 2 cut(s) | 165, 744 |
| PsyI | GACNNNGTC | 1 cut(s) | 1237 |
| PvuII | CAGCTG | 2 cut(s) | 241, 437 |
| RruI | TCGCGA | 1 cut(s) | 288 |
| RsaI | GTAC | 5 cut(s) | 50, 459, 1220, 1365, 1501 |
| RsaNI | GTAC | 5 cut(s) | 49, 458, 1219, 1364, 1500 |
| RseI | CAYNNNNRTG | 3 cut(s) | 366, 471, 1091 |
| SacI | GAGCTC | 1 cut(s) | 864 |
| SacII | CCGCGG | 3 cut(s) | 963, 973, 1377 |
| SalI | GTCGAC | 1 cut(s) | 233 |
| SaqAI | TTAA | 1 cut(s) | 90 |
| Sau3AI | GATC | 3 cut(s) | 165, 744, 1537 |
| SchI | GAGTC | 6 cut(s) | 292, 329, 1112, 1247, 1432, 1525 |
| ScrFI | CCNGG | 7 cut(s) | 53, 270, 603, 1331, 1418, 1475, 1490 |
| SduI | GDGCHC | 6 cut(s) | 76, 864, 1258, 1329, 1345, 1414 |
| SfaNI | GCATC | 2 cut(s) | 330, 336 |
| SfcI | CTRYAG | 3 cut(s) | 778, 884, 1052 |
| SfoI | GGCGCC | 1 cut(s) | 708 |
| Sfr303I | CCGCGG | 3 cut(s) | 963, 973, 1377 |
| SgrAI | CRCCGGYG | 1 cut(s) | 538 |
| SgrBI | CCGCGG | 3 cut(s) | 963, 973, 1377 |
| SinI | GGWCC | 4 cut(s) | 18, 310, 476, 646 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 366, 471, 1091 |
| SmlI | CTYRAG | 1 cut(s) | 581 |
| SmoI | CTYRAG | 1 cut(s) | 581 |
| SphI | GCATGC | 1 cut(s) | 1571 |
| Sse9I | AATT | 1 cut(s) | 87 |
| SspDI | GGCGCC | 1 cut(s) | 706 |
| SspMI | CTAG | 2 cut(s) | 740, 1259 |
| SstI | GAGCTC | 1 cut(s) | 864 |
| StyD4I | CCNGG | 7 cut(s) | 51, 268, 601, 1329, 1416, 1473, 1488 |
| StyI | CCWWGG | 3 cut(s) | 524, 572, 1528 |
| TaaI | ACNGT | 7 cut(s) | 314, 377, 496, 514, 953, 1209, 1499 |
| TaiI | ACGT | 3 cut(s) | 83, 1179, 1360 |
| TaqI | TCGA | 4 cut(s) | 205, 234, 852, 1296 |
| TaqII | GACCGA | 1 cut(s) | 1250 |
| TasI | AATT | 1 cut(s) | 87 |
| TatI | WGTACW | 1 cut(s) | 1218 |
| TfiI | GAWTC | 3 cut(s) | 184, 568, 727 |
| Tru1I | TTAA | 1 cut(s) | 90 |
| Tru9I | TTAA | 1 cut(s) | 90 |
| TscAI | CASTG | 3 cut(s) | 411, 523, 1402 |
| TseFI | GTSAC | 2 cut(s) | 514, 953 |
| TseI | GCWGC | 8 cut(s) | 241, 437, 499, 686, 811, 841, 1051, 1096 |
| Tsp45I | GTSAC | 2 cut(s) | 514, 953 |
| TspDTI | ATGAA | 1 cut(s) | 1029 |
| TspGWI | ACGGA | 4 cut(s) | 178, 244, 638, 1136 |
| TspRI | CASTG | 3 cut(s) | 411, 523, 1402 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1237 |
| VpaK11BI | GGWCC | 4 cut(s) | 18, 310, 476, 646 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1052 |
| XceI | RCATGY | 1 cut(s) | 1571 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 362 |
| XmiI | GTMKAC | 2 cut(s) | 234, 883 |
| XspI | CTAG | 2 cut(s) | 740, 1259 |