Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 472 |
| Acc36I | ACCTGC | 3 cut(s) | 187, 472, 712 |
| Acc65I | GGTACC | 1 cut(s) | 822 |
| AccB1I | GGYRCC | 3 cut(s) | 822, 977, 1681 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 313, 1539 |
| AccBSI | CCGCTC | 2 cut(s) | 1321, 1413 |
| AccI | GTMKAC | 1 cut(s) | 617 |
| AccII | CGCG | 5 cut(s) | 162, 927, 1284, 1763, 1798 |
| AclI | AACGTT | 1 cut(s) | 1713 |
| AclWI | GGATC | 3 cut(s) | 888, 901, 1731 |
| AcoI | YGGCCR | 1 cut(s) | 902 |
| AcsI | RAATTY | 2 cut(s) | 526, 638 |
| AcuI | CTGAAG | 2 cut(s) | 230, 1328 |
| AcyI | GRCGYC | 1 cut(s) | 1550 |
| AdeI | CACNNNGTG | 2 cut(s) | 262, 730 |
| AfaI | GTAC | 5 cut(s) | 824, 1067, 1460, 1729, 1855 |
| AfeI | AGCGCT | 2 cut(s) | 188, 1529 |
| AflIII | ACRYGT | 1 cut(s) | 357 |
| AgsI | TTSAA | 4 cut(s) | 532, 850, 914, 1361 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1152 |
| AjnI | CCWGG | 7 cut(s) | 149, 1056, 1077, 1553, 1588, 1668, 1730 |
| AleI | CACNNNNGTG | 1 cut(s) | 1113 |
| Alw21I | GWGCWC | 3 cut(s) | 175, 258, 1188 |
| Alw26I | GTCTC | 3 cut(s) | 951, 962, 1705 |
| Alw44I | GTGCAC | 1 cut(s) | 254 |
| AlwI | GGATC | 3 cut(s) | 888, 901, 1731 |
| Aor51HI | AGCGCT | 2 cut(s) | 188, 1529 |
| AoxI | GGCC | 8 cut(s) | 328, 802, 860, 902, 1221, 1422, 1488, 1707 |
| ApaLI | GTGCAC | 1 cut(s) | 254 |
| ApeKI | GCWGC | 7 cut(s) | 197, 460, 673, 1169, 1172, 1246, 1388 |
| ApoI | RAATTY | 2 cut(s) | 526, 638 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 907, 939 |
| AseI | ATTAAT | 1 cut(s) | 449 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1006 |
| Asp718I | GGTACC | 1 cut(s) | 822 |
| AspA2I | CCTAGG | 1 cut(s) | 1571 |
| AspLEI | GCGC | 7 cut(s) | 92, 189, 379, 1530, 1626, 1763, 1765 |
| AspS9I | GGNCC | 8 cut(s) | 558, 802, 860, 1146, 1557, 1599, 1707, 1743 |
| AsuC2I | CCSGG | 1 cut(s) | 1705 |
| AsuHPI | GGTGA | 8 cut(s) | 127, 274, 496, 537, 834, 1030, 1171, 1853 |
| AsuNHI | GCTAGC | 3 cut(s) | 95, 235, 790 |
| AvaII | GGWCC | 5 cut(s) | 558, 1146, 1557, 1599, 1743 |
| AvrII | CCTAGG | 1 cut(s) | 1571 |
| BaeGI | GKGCMC | 2 cut(s) | 258, 982 |
| BamHI | GGATCC | 1 cut(s) | 893 |
| BanI | GGYRCC | 3 cut(s) | 822, 977, 1681 |
| BanII | GRGCYC | 5 cut(s) | 513, 602, 767, 1188, 1796 |
| BauI | CACGAG | 2 cut(s) | 257, 760 |
| Bbv12I | GWGCWC | 3 cut(s) | 175, 258, 1188 |
| BbvI | GCAGC | 7 cut(s) | 184, 472, 660, 1156, 1184, 1258, 1375 |
| BccI | CCATC | 9 cut(s) | 104, 367, 576, 944, 980, 1136, 1310, 1468, 1754 |
| BceAI | ACGGC | 5 cut(s) | 32, 236, 917, 1051, 1564 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 152, 186 |
| BciT130I | CCWGG | 7 cut(s) | 151, 1058, 1079, 1555, 1590, 1670, 1732 |
| BclI | TGATCA | 1 cut(s) | 1327 |
| BcnI | CCSGG | 1 cut(s) | 1705 |
| BcoDI | GTCTC | 3 cut(s) | 951, 962, 1705 |
| BfaI | CTAG | 9 cut(s) | 30, 96, 236, 708, 791, 1124, 1229, 1572, 1758 |
| BfmI | CTRYAG | 2 cut(s) | 472, 1170 |
| BfoI | RGCGCY | 4 cut(s) | 93, 190, 380, 1531 |
| BfuAI | ACCTGC | 3 cut(s) | 187, 472, 712 |
| BglII | AGATCT | 1 cut(s) | 710 |
| BlnI | CCTAGG | 1 cut(s) | 1571 |
| BlpI | GCTNAGC | 1 cut(s) | 1250 |
| Bme1390I | CCNGG | 8 cut(s) | 151, 1058, 1079, 1555, 1590, 1670, 1705, 1732 |
| Bme18I | GGWCC | 5 cut(s) | 558, 1146, 1557, 1599, 1743 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1152 |
| BmgT120I | GGNCC | 8 cut(s) | 558, 802, 860, 1146, 1557, 1599, 1707, 1743 |
| BmrFI | CCNGG | 8 cut(s) | 151, 1058, 1079, 1555, 1590, 1670, 1705, 1732 |
| BmrI | ACTGGG | 1 cut(s) | 1684 |
| BmsI | GCATC | 9 cut(s) | 34, 154, 192, 922, 952, 1258, 1267, 1426, 1468 |
| BmtI | GCTAGC | 3 cut(s) | 99, 239, 794 |
| BmuI | ACTGGG | 1 cut(s) | 1684 |
| BpmI | CTGGAG | 2 cut(s) | 290, 1418 |
| Bpu10I | CCTNAGC | 1 cut(s) | 677 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1250 |
| BpuEI | CTTGAG | 2 cut(s) | 1115, 1337 |
| BpuMI | CCSGG | 1 cut(s) | 1705 |
| Bsa29I | ATCGAT | 1 cut(s) | 939 |
| BsaAI | YACGTR | 1 cut(s) | 457 |
| BsaHI | GRCGYC | 1 cut(s) | 1550 |
| BsaI | GGTCTC | 2 cut(s) | 962, 1705 |
| BsaWI | WCCGGW | 2 cut(s) | 1087, 1678 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1082, 1112 |
| Bse118I | RCCGGY | 5 cut(s) | 239, 828, 904, 1490, 1616 |
| Bse1I | ACTGG | 4 cut(s) | 895, 1401, 1690, 1745 |
| BseBI | CCWGG | 7 cut(s) | 151, 1058, 1079, 1555, 1590, 1670, 1732 |
| BseCI | ATCGAT | 1 cut(s) | 939 |
| BseGI | GGATG | 7 cut(s) | 49, 220, 1147, 1273, 1282, 1441, 1483 |
| BseMII | CTCAG | 6 cut(s) | 12, 482, 691, 1232, 1241, 1686 |
| BseNI | ACTGG | 4 cut(s) | 895, 1401, 1690, 1745 |
| BsePI | GCGCGC | 1 cut(s) | 1761 |
| BseRI | GAGGAG | 5 cut(s) | 883, 1415, 1418, 1423, 1835 |
| BseSI | GKGCMC | 2 cut(s) | 258, 982 |
| BseX3I | CGGCCG | 1 cut(s) | 902 |
| BseXI | GCAGC | 7 cut(s) | 184, 472, 660, 1156, 1184, 1258, 1375 |
| BseYI | CCCAGC | 1 cut(s) | 1654 |
| BsgI | GTGCAG | 1 cut(s) | 479 |
| Bsh1236I | CGCG | 5 cut(s) | 162, 927, 1284, 1763, 1798 |
| Bsh1285I | CGRYCG | 1 cut(s) | 905 |
| BshFI | GGCC | 8 cut(s) | 330, 804, 862, 904, 1223, 1424, 1490, 1709 |
| BshNI | GGYRCC | 3 cut(s) | 822, 977, 1681 |
| BshVI | ATCGAT | 1 cut(s) | 939 |
| BsiEI | CGRYCG | 1 cut(s) | 905 |
| BsiHKAI | GWGCWC | 3 cut(s) | 175, 258, 1188 |
| BsiSI | CCGG | 9 cut(s) | 240, 829, 905, 1088, 1491, 1617, 1629, 1679, 1704 |
| BsiWI | CGTACG | 1 cut(s) | 1065 |
| BslFI | GGGAC | 2 cut(s) | 571, 1159 |
| BsmAI | GTCTC | 3 cut(s) | 951, 962, 1705 |
| BsmBI | CGTCTC | 1 cut(s) | 951 |
| BsmFI | GGGAC | 2 cut(s) | 571, 1159 |
| BsmI | GAATGC | 1 cut(s) | 788 |
| BsnI | GGCC | 8 cut(s) | 330, 804, 862, 904, 1223, 1424, 1490, 1709 |
| Bso31I | GGTCTC | 2 cut(s) | 962, 1705 |
| Bsp1286I | GDGCHC | 8 cut(s) | 175, 258, 513, 602, 767, 982, 1188, 1796 |
| Bsp143I | GATC | 5 cut(s) | 264, 710, 893, 1327, 1723 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1250 |
| Bsp68I | TCGCGA | 1 cut(s) | 927 |
| BspANI | GGCC | 8 cut(s) | 330, 804, 862, 904, 1223, 1424, 1490, 1709 |
| BspCNI | CTCAG | 6 cut(s) | 13, 483, 690, 1231, 1242, 1687 |
| BspDI | ATCGAT | 1 cut(s) | 939 |
| BspFNI | CGCG | 5 cut(s) | 162, 927, 1284, 1763, 1798 |
| BspHI | TCATGA | 1 cut(s) | 1749 |
| BspMAI | CTGCAG | 1 cut(s) | 1174 |
| BspMI | ACCTGC | 3 cut(s) | 187, 472, 712 |
| BspOI | GCTAGC | 3 cut(s) | 99, 239, 794 |
| BspPI | GGATC | 3 cut(s) | 888, 901, 1731 |
| BspT107I | GGYRCC | 3 cut(s) | 822, 977, 1681 |
| BspTNI | GGTCTC | 2 cut(s) | 962, 1705 |
| BsrBI | CCGCTC | 2 cut(s) | 1321, 1413 |
| BsrFI | RCCGGY | 5 cut(s) | 239, 828, 904, 1490, 1616 |
| BsrI | ACTGG | 4 cut(s) | 895, 1401, 1690, 1745 |
| BssAI | RCCGGY | 5 cut(s) | 239, 828, 904, 1490, 1616 |
| BssHII | GCGCGC | 1 cut(s) | 1761 |
| BssMI | GATC | 5 cut(s) | 264, 710, 893, 1327, 1723 |
| BssNI | GRCGYC | 1 cut(s) | 1550 |
| BssSI | CACGAG | 2 cut(s) | 257, 760 |
| BssT1I | CCWWGG | 6 cut(s) | 274, 466, 548, 1014, 1571, 1602 |
| Bst2BI | CACGAG | 2 cut(s) | 257, 760 |
| Bst2UI | CCWGG | 7 cut(s) | 151, 1058, 1079, 1555, 1590, 1670, 1732 |
| Bst4CI | ACNGT | 4 cut(s) | 407, 421, 1150, 1166 |
| Bst6I | CTCTTC | 2 cut(s) | 63, 942 |
| BstACI | GRCGYC | 1 cut(s) | 1550 |
| BstBAI | YACGTR | 1 cut(s) | 457 |
| BstDEI | CTNAG | 6 cut(s) | 21, 491, 677, 1218, 1250, 1695 |
| BstEII | GGTNACC | 1 cut(s) | 1018 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 651 |
| BstF5I | GGATG | 7 cut(s) | 49, 220, 1147, 1273, 1282, 1441, 1483 |
| BstFNI | CGCG | 5 cut(s) | 162, 927, 1284, 1763, 1798 |
| BstH2I | RGCGCY | 4 cut(s) | 93, 190, 380, 1531 |
| BstHHI | GCGC | 7 cut(s) | 92, 189, 379, 1530, 1626, 1763, 1765 |
| BstKTI | GATC | 5 cut(s) | 267, 713, 896, 1330, 1726 |
| BstMAI | GTCTC | 3 cut(s) | 951, 962, 1705 |
| BstMBI | GATC | 5 cut(s) | 264, 710, 893, 1327, 1723 |
| BstMCI | CGRYCG | 1 cut(s) | 905 |
| BstNI | CCWGG | 7 cut(s) | 151, 1058, 1079, 1555, 1590, 1670, 1732 |
| BstNSI | RCATGY | 1 cut(s) | 361 |
| BstPI | GGTNACC | 1 cut(s) | 1018 |
| BstSCI | CCNGG | 8 cut(s) | 149, 1056, 1077, 1553, 1588, 1668, 1703, 1730 |
| BstSFI | CTRYAG | 2 cut(s) | 472, 1170 |
| BstSLI | GKGCMC | 2 cut(s) | 258, 982 |
| BstUI | CGCG | 5 cut(s) | 162, 927, 1284, 1763, 1798 |
| BstV1I | GCAGC | 7 cut(s) | 184, 472, 660, 1156, 1184, 1258, 1375 |
| BstX2I | RGATCY | 2 cut(s) | 710, 893 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1717 |
| BstYI | RGATCY | 2 cut(s) | 710, 893 |
| BstZI | CGGCCG | 1 cut(s) | 902 |
| Bsu15I | ATCGAT | 1 cut(s) | 939 |
| BsuRI | GGCC | 8 cut(s) | 330, 804, 862, 904, 1223, 1424, 1490, 1709 |
| BsuTUI | ATCGAT | 1 cut(s) | 939 |
| BtgZI | GCGATG | 2 cut(s) | 177, 215 |
| BtsCI | GGATG | 7 cut(s) | 49, 220, 1147, 1273, 1282, 1441, 1483 |
| BtsI | GCAGTG | 2 cut(s) | 102, 1639 |
| BtsIMutI | CAGTG | 3 cut(s) | 102, 888, 1639 |
| BtuMI | TCGCGA | 1 cut(s) | 927 |
| BveI | ACCTGC | 3 cut(s) | 187, 472, 712 |
| CciI | TCATGA | 1 cut(s) | 1749 |
| CfoI | GCGC | 7 cut(s) | 92, 189, 379, 1530, 1626, 1763, 1765 |
| Cfr10I | RCCGGY | 5 cut(s) | 239, 828, 904, 1490, 1616 |
| Cfr13I | GGNCC | 8 cut(s) | 558, 802, 860, 1146, 1557, 1599, 1707, 1743 |
| ClaI | ATCGAT | 1 cut(s) | 939 |
| CseI | GACGC | 3 cut(s) | 213, 911, 1539 |
| CsiI | ACCWGGT | 1 cut(s) | 1730 |
| Csp6I | GTAC | 5 cut(s) | 823, 1066, 1459, 1728, 1854 |
| CviAII | CATG | 5 cut(s) | 245, 358, 541, 1750, 1766 |
| CviQI | GTAC | 5 cut(s) | 823, 1066, 1459, 1728, 1854 |
| DdeI | CTNAG | 6 cut(s) | 21, 491, 677, 1218, 1250, 1695 |
| DpnI | GATC | 5 cut(s) | 266, 712, 895, 1329, 1725 |
| DpnII | GATC | 5 cut(s) | 264, 710, 893, 1327, 1723 |
| DraIII | CACNNNGTG | 2 cut(s) | 262, 730 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1152 |
| EaeI | YGGCCR | 1 cut(s) | 902 |
| EagI | CGGCCG | 1 cut(s) | 902 |
| Eam1104I | CTCTTC | 2 cut(s) | 63, 942 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1152 |
| EarI | CTCTTC | 2 cut(s) | 63, 942 |
| EciI | GGCGGA | 7 cut(s) | 260, 847, 874, 1226, 1274, 1549, 1736 |
| Ecl136II | GAGCTC | 1 cut(s) | 1186 |
| EclXI | CGGCCG | 1 cut(s) | 902 |
| Eco130I | CCWWGG | 6 cut(s) | 274, 466, 548, 1014, 1571, 1602 |
| Eco24I | GRGCYC | 5 cut(s) | 513, 602, 767, 1188, 1796 |
| Eco31I | GGTCTC | 2 cut(s) | 962, 1705 |
| Eco47I | GGWCC | 5 cut(s) | 558, 1146, 1557, 1599, 1743 |
| Eco47III | AGCGCT | 2 cut(s) | 188, 1529 |
| Eco52I | CGGCCG | 1 cut(s) | 902 |
| Eco53kI | GAGCTC | 1 cut(s) | 1186 |
| Eco57I | CTGAAG | 2 cut(s) | 230, 1328 |
| Eco91I | GGTNACC | 1 cut(s) | 1018 |
| EcoICRI | GAGCTC | 1 cut(s) | 1186 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 651 |
| EcoO109I | RGGNCCY | 2 cut(s) | 558, 1599 |
| EcoO65I | GGTNACC | 1 cut(s) | 1018 |
| EcoRI | GAATTC | 1 cut(s) | 638 |
| EcoRII | CCWGG | 7 cut(s) | 149, 1056, 1077, 1553, 1588, 1668, 1730 |
| EcoT14I | CCWWGG | 6 cut(s) | 274, 466, 548, 1014, 1571, 1602 |
| EcoT38I | GRGCYC | 5 cut(s) | 513, 602, 767, 1188, 1796 |
| ErhI | CCWWGG | 6 cut(s) | 274, 466, 548, 1014, 1571, 1602 |
| Esp3I | CGTCTC | 1 cut(s) | 951 |
| FaeI | CATG | 5 cut(s) | 248, 361, 544, 1753, 1769 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 991, 1023 |
| FaqI | GGGAC | 2 cut(s) | 571, 1159 |
| FatI | CATG | 5 cut(s) | 244, 357, 540, 1749, 1765 |
| FauI | CCCGC | 9 cut(s) | 57, 160, 297, 546, 589, 795, 989, 1191, 1589 |
| FbaI | TGATCA | 1 cut(s) | 1327 |
| FblI | GTMKAC | 1 cut(s) | 617 |
| FokI | GGATG | 7 cut(s) | 56, 227, 1154, 1280, 1289, 1448, 1490 |
| FriOI | GRGCYC | 5 cut(s) | 513, 602, 767, 1188, 1796 |
| FspBI | CTAG | 9 cut(s) | 30, 96, 236, 708, 791, 1124, 1229, 1572, 1758 |
| GlaI | GCGC | 7 cut(s) | 91, 188, 378, 1529, 1625, 1762, 1764 |
| GsaI | CCCAGC | 1 cut(s) | 1658 |
| GsuI | CTGGAG | 2 cut(s) | 290, 1418 |
| HaeII | RGCGCY | 4 cut(s) | 93, 190, 380, 1531 |
| HaeIII | GGCC | 8 cut(s) | 330, 804, 862, 904, 1223, 1424, 1490, 1709 |
| HapII | CCGG | 9 cut(s) | 240, 829, 905, 1088, 1491, 1617, 1629, 1679, 1704 |
| HgaI | GACGC | 3 cut(s) | 213, 911, 1539 |
| HhaI | GCGC | 7 cut(s) | 92, 189, 379, 1530, 1626, 1763, 1765 |
| Hin1I | GRCGYC | 1 cut(s) | 1550 |
| Hin1II | CATG | 5 cut(s) | 248, 361, 544, 1753, 1769 |
| Hin6I | GCGC | 7 cut(s) | 90, 187, 377, 1528, 1624, 1761, 1763 |
| HinP1I | GCGC | 7 cut(s) | 90, 187, 377, 1528, 1624, 1761, 1763 |
| HincII | GTYRAC | 1 cut(s) | 618 |
| HindII | GTYRAC | 1 cut(s) | 618 |
| HindIII | AAGCTT | 1 cut(s) | 1467 |
| HinfI | GANTC | 8 cut(s) | 143, 412, 493, 536, 614, 1061, 1128, 1214 |
| HpaII | CCGG | 9 cut(s) | 240, 829, 905, 1088, 1491, 1617, 1629, 1679, 1704 |
| HphI | GGTGA | 8 cut(s) | 127, 274, 496, 537, 834, 1030, 1171, 1853 |
| Hpy166II | GTNNAC | 4 cut(s) | 256, 319, 487, 618 |
| Hpy188I | TCNGA | 7 cut(s) | 637, 1276, 1518, 1614, 1696, 1738, 1774 |
| Hpy188III | TCNNGA | 6 cut(s) | 20, 307, 625, 679, 926, 1750 |
| Hpy8I | GTNNAC | 4 cut(s) | 256, 319, 487, 618 |
| Hpy99I | CGWCG | 3 cut(s) | 229, 927, 1136 |
| HpyAV | CCTTC | 8 cut(s) | 205, 526, 571, 844, 947, 988, 1480, 1540 |
| HpyCH4III | ACNGT | 4 cut(s) | 407, 421, 1150, 1166 |
| HpyCH4IV | ACGT | 3 cut(s) | 456, 1506, 1713 |
| HpyCH4V | TGCA | 6 cut(s) | 256, 460, 771, 1045, 1108, 1172 |
| HpyF3I | CTNAG | 6 cut(s) | 21, 491, 677, 1218, 1250, 1695 |
| HpySE526I | ACGT | 3 cut(s) | 456, 1506, 1713 |
| Hsp92I | GRCGYC | 1 cut(s) | 1550 |
| Hsp92II | CATG | 5 cut(s) | 248, 361, 544, 1753, 1769 |
| HspAI | GCGC | 7 cut(s) | 90, 187, 377, 1528, 1624, 1761, 1763 |
| KpnI | GGTACC | 1 cut(s) | 826 |
| KroI | GCCGGC | 1 cut(s) | 828 |
| KroNI | GCCGGC | 1 cut(s) | 830 |
| Ksp22I | TGATCA | 1 cut(s) | 1327 |
| Kzo9I | GATC | 5 cut(s) | 264, 710, 893, 1327, 1723 |
| LmnI | GCTCC | 8 cut(s) | 468, 508, 1090, 1410, 1535, 1631, 1775, 1822 |
| Lsp1109I | GCAGC | 7 cut(s) | 184, 472, 660, 1156, 1184, 1258, 1375 |
| LweI | GCATC | 9 cut(s) | 34, 154, 192, 922, 952, 1258, 1267, 1426, 1468 |
| MabI | ACCWGGT | 1 cut(s) | 1730 |
| MaeI | CTAG | 9 cut(s) | 30, 96, 236, 708, 791, 1124, 1229, 1572, 1758 |
| MaeII | ACGT | 3 cut(s) | 456, 1506, 1713 |
| MaeIII | GTNAC | 7 cut(s) | 49, 280, 432, 543, 840, 1018, 1177 |
| MalI | GATC | 5 cut(s) | 266, 712, 895, 1329, 1725 |
| MbiI | CCGCTC | 2 cut(s) | 1321, 1413 |
| MboI | GATC | 5 cut(s) | 264, 710, 893, 1327, 1723 |
| MboII | GAAGA | 7 cut(s) | 80, 259, 705, 941, 956, 959, 1003 |
| MflI | RGATCY | 2 cut(s) | 710, 893 |
| MhlI | GDGCHC | 8 cut(s) | 175, 258, 513, 602, 767, 982, 1188, 1796 |
| MluCI | AATT | 7 cut(s) | 446, 515, 526, 638, 1205, 1296, 1861 |
| MlyI | GAGTC | 5 cut(s) | 152, 421, 502, 623, 1055 |
| MmeI | TCCRAC | 8 cut(s) | 426, 440, 615, 667, 1185, 1527, 1716, 1797 |
| MreI | CGCCGGCG | 1 cut(s) | 828 |
| MroNI | GCCGGC | 1 cut(s) | 828 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1006 |
| MseI | TTAA | 3 cut(s) | 449, 630, 1443 |
| MslI | CAYNNNNRTG | 2 cut(s) | 441, 1113 |
| MspA1I | CMGCKG | 6 cut(s) | 208, 673, 755, 1249, 1430, 1658 |
| MspI | CCGG | 9 cut(s) | 240, 829, 905, 1088, 1491, 1617, 1629, 1679, 1704 |
| MspR9I | CCNGG | 8 cut(s) | 151, 1058, 1079, 1555, 1590, 1670, 1705, 1732 |
| Mva1269I | GAATGC | 1 cut(s) | 788 |
| MvaI | CCWGG | 7 cut(s) | 151, 1058, 1079, 1555, 1590, 1670, 1732 |
| MvnI | CGCG | 5 cut(s) | 162, 927, 1284, 1763, 1798 |
| NaeI | GCCGGC | 1 cut(s) | 830 |
| NciI | CCSGG | 1 cut(s) | 1705 |
| NdeII | GATC | 5 cut(s) | 264, 710, 893, 1327, 1723 |
| NgoMIV | GCCGGC | 1 cut(s) | 828 |
| NheI | GCTAGC | 3 cut(s) | 95, 235, 790 |
| NlaIII | CATG | 5 cut(s) | 248, 361, 544, 1753, 1769 |
| NmeAIII | GCCGAG | 2 cut(s) | 1096, 1837 |
| NmuCI | GTSAC | 5 cut(s) | 280, 543, 840, 1018, 1177 |
| NruI | TCGCGA | 1 cut(s) | 927 |
| NspI | RCATGY | 1 cut(s) | 361 |
| OliI | CACNNNNGTG | 1 cut(s) | 1113 |
| PagI | TCATGA | 1 cut(s) | 1749 |
| PaqCI | CACCTGC | 1 cut(s) | 472 |
| PasI | CCCWGGG | 2 cut(s) | 1589, 1669 |
| PauI | GCGCGC | 1 cut(s) | 1761 |
| PciI | ACATGT | 1 cut(s) | 357 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 13 |
| PctI | GAATGC | 1 cut(s) | 788 |
| PdiI | GCCGGC | 1 cut(s) | 830 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1006 |
| PfeI | GAWTC | 3 cut(s) | 536, 1128, 1214 |
| Pfl23II | CGTACG | 1 cut(s) | 1065 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 313, 1539 |
| PleI | GAGTC | 5 cut(s) | 151, 420, 501, 622, 1055 |
| PpsI | GAGTC | 5 cut(s) | 151, 420, 501, 622, 1055 |
| Ppu21I | YACGTR | 1 cut(s) | 457 |
| PpuMI | RGGWCCY | 2 cut(s) | 558, 1599 |
| PscI | ACATGT | 1 cut(s) | 357 |
| PshBI | ATTAAT | 1 cut(s) | 449 |
| Psp124BI | GAGCTC | 1 cut(s) | 1188 |
| Psp1406I | AACGTT | 1 cut(s) | 1713 |
| Psp5II | RGGWCCY | 2 cut(s) | 558, 1599 |
| Psp6I | CCWGG | 7 cut(s) | 149, 1056, 1077, 1553, 1588, 1668, 1730 |
| PspEI | GGTNACC | 1 cut(s) | 1018 |
| PspFI | CCCAGC | 1 cut(s) | 1654 |
| PspGI | CCWGG | 7 cut(s) | 149, 1056, 1077, 1553, 1588, 1668, 1730 |
| PspLI | CGTACG | 1 cut(s) | 1065 |
| PspPI | GGNCC | 8 cut(s) | 558, 802, 860, 1146, 1557, 1599, 1707, 1743 |
| PspPPI | RGGWCCY | 2 cut(s) | 558, 1599 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1058, 1090 |
| PstI | CTGCAG | 1 cut(s) | 1174 |
| PsuI | RGATCY | 2 cut(s) | 710, 893 |
| PteI | GCGCGC | 1 cut(s) | 1761 |
| PvuII | CAGCTG | 2 cut(s) | 755, 1249 |
| RruI | TCGCGA | 1 cut(s) | 927 |
| RsaI | GTAC | 5 cut(s) | 824, 1067, 1460, 1729, 1855 |
| RsaNI | GTAC | 5 cut(s) | 823, 1066, 1459, 1728, 1854 |
| RseI | CAYNNNNRTG | 2 cut(s) | 441, 1113 |
| SacI | GAGCTC | 1 cut(s) | 1188 |
| SalI | GTCGAC | 1 cut(s) | 616 |
| SaqAI | TTAA | 3 cut(s) | 449, 630, 1443 |
| Sau3AI | GATC | 5 cut(s) | 264, 710, 893, 1327, 1723 |
| Sau96I | GGNCC | 8 cut(s) | 558, 802, 860, 1146, 1557, 1599, 1707, 1743 |
| SchI | GAGTC | 5 cut(s) | 152, 421, 502, 623, 1055 |
| ScrFI | CCNGG | 8 cut(s) | 151, 1058, 1079, 1555, 1590, 1670, 1705, 1732 |
| SduI | GDGCHC | 8 cut(s) | 175, 258, 513, 602, 767, 982, 1188, 1796 |
| SexAI | ACCWGGT | 1 cut(s) | 1730 |
| SfaNI | GCATC | 9 cut(s) | 34, 154, 192, 922, 952, 1258, 1267, 1426, 1468 |
| SfcI | CTRYAG | 2 cut(s) | 472, 1170 |
| SgrAI | CRCCGGYG | 1 cut(s) | 828 |
| SinI | GGWCC | 5 cut(s) | 558, 1146, 1557, 1599, 1743 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 441, 1113 |
| SmlI | CTYRAG | 2 cut(s) | 1094, 1316 |
| SmoI | CTYRAG | 2 cut(s) | 1094, 1316 |
| Sse9I | AATT | 7 cut(s) | 446, 515, 526, 638, 1205, 1296, 1861 |
| SspMI | CTAG | 9 cut(s) | 30, 96, 236, 708, 791, 1124, 1229, 1572, 1758 |
| SstI | GAGCTC | 1 cut(s) | 1188 |
| StyD4I | CCNGG | 8 cut(s) | 149, 1056, 1077, 1553, 1588, 1668, 1703, 1730 |
| StyI | CCWWGG | 6 cut(s) | 274, 466, 548, 1014, 1571, 1602 |
| TaaI | ACNGT | 4 cut(s) | 407, 421, 1150, 1166 |
| TaiI | ACGT | 3 cut(s) | 459, 1509, 1716 |
| TaqI | TCGA | 9 cut(s) | 602, 617, 652, 875, 939, 1131, 1188, 1305, 1836 |
| TaqII | GACCGA | 1 cut(s) | 217 |
| TasI | AATT | 7 cut(s) | 446, 515, 526, 638, 1205, 1296, 1861 |
| TfiI | GAWTC | 3 cut(s) | 536, 1128, 1214 |
| Tru1I | TTAA | 3 cut(s) | 449, 630, 1443 |
| Tru9I | TTAA | 3 cut(s) | 449, 630, 1443 |
| TscAI | CASTG | 3 cut(s) | 109, 895, 1639 |
| TseFI | GTSAC | 5 cut(s) | 280, 543, 840, 1018, 1177 |
| TseI | GCWGC | 7 cut(s) | 197, 460, 673, 1169, 1172, 1246, 1388 |
| Tsp45I | GTSAC | 5 cut(s) | 280, 543, 840, 1018, 1177 |
| TspDTI | ATGAA | 2 cut(s) | 459, 957 |
| TspGWI | ACGGA | 3 cut(s) | 1142, 1146, 1149 |
| TspRI | CASTG | 3 cut(s) | 109, 895, 1639 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 313, 1539 |
| VneI | GTGCAC | 1 cut(s) | 254 |
| VpaK11BI | GGWCC | 5 cut(s) | 558, 1146, 1557, 1599, 1743 |
| VspI | ATTAAT | 1 cut(s) | 449 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 651 |
| XapI | RAATTY | 2 cut(s) | 526, 638 |
| XceI | RCATGY | 1 cut(s) | 361 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 473 |
| XmaJI | CCTAGG | 1 cut(s) | 1571 |
| XmiI | GTMKAC | 1 cut(s) | 617 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1006 |
| XspI | CTAG | 9 cut(s) | 30, 96, 236, 708, 791, 1124, 1229, 1572, 1758 |