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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 3 cut(s) | 100, 695, 1003 |
| Acc36I | ACCTGC | 1 cut(s) | 947 |
| Acc65I | GGTACC | 1 cut(s) | 1082 |
| AccB1I | GGYRCC | 3 cut(s) | 205, 866, 1082 |
| AccBSI | CCGCTC | 4 cut(s) | 268, 295, 508, 637 |
| AccI | GTMKAC | 1 cut(s) | 683 |
| AccII | CGCG | 3 cut(s) | 549, 597, 1111 |
| AcoI | YGGCCR | 2 cut(s) | 564, 759 |
| AcuI | CTGAAG | 2 cut(s) | 340, 762 |
| AcvI | CACGTG | 1 cut(s) | 1046 |
| AcyI | GRCGYC | 4 cut(s) | 206, 410, 543, 867 |
| AfaI | GTAC | 4 cut(s) | 751, 903, 961, 1084 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 60, 430, 573, 956, 1001, 1036 |
| AflIII | ACRYGT | 2 cut(s) | 595, 849 |
| AgsI | TTSAA | 5 cut(s) | 773, 781, 856, 1157, 1172 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1098 |
| AjnI | CCWGG | 2 cut(s) | 1056, 1071 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 257, 289 |
| AluBI | AGCT | 6 cut(s) | 223, 360, 439, 556, 740, 953 |
| AluI | AGCT | 6 cut(s) | 223, 360, 439, 556, 740, 953 |
| Alw21I | GWGCWC | 1 cut(s) | 441 |
| Alw26I | GTCTC | 5 cut(s) | 108, 464, 604, 923, 1085 |
| Ama87I | CYCGRG | 2 cut(s) | 834, 967 |
| AoxI | GGCC | 6 cut(s) | 144, 162, 564, 759, 831, 985 |
| ApaI | GGGCCC | 2 cut(s) | 148, 835 |
| ApeKI | GCWGC | 6 cut(s) | 418, 628, 673, 755, 953, 972 |
| Asp718I | GGTACC | 1 cut(s) | 1082 |
| AspLEI | GCGC | 6 cut(s) | 76, 90, 208, 869, 1066, 1111 |
| AspS9I | GGNCC | 9 cut(s) | 51, 144, 145, 163, 831, 832, 986, 1005, 1116 |
| AsuC2I | CCSGG | 3 cut(s) | 424, 835, 836 |
| AsuHPI | GGTGA | 2 cut(s) | 55, 731 |
| AsuII | TTCGAA | 1 cut(s) | 179 |
| AvaI | CYCGRG | 2 cut(s) | 834, 967 |
| AvaII | GGWCC | 3 cut(s) | 51, 1005, 1116 |
| BaeGI | GKGCMC | 2 cut(s) | 148, 835 |
| BanI | GGYRCC | 3 cut(s) | 205, 866, 1082 |
| BanII | GRGCYC | 3 cut(s) | 148, 441, 835 |
| BbrPI | CACGTG | 1 cut(s) | 1046 |
| BbsI | GAAGAC | 2 cut(s) | 188, 809 |
| Bbv12I | GWGCWC | 1 cut(s) | 441 |
| BbvI | GCAGC | 6 cut(s) | 430, 615, 685, 742, 940, 984 |
| BccI | CCATC | 4 cut(s) | 291, 575, 688, 1073 |
| BceAI | ACGGC | 2 cut(s) | 111, 351 |
| BciT130I | CCWGG | 2 cut(s) | 1058, 1073 |
| BcnI | CCSGG | 3 cut(s) | 424, 835, 836 |
| BcoDI | GTCTC | 5 cut(s) | 108, 464, 604, 923, 1085 |
| BfaI | CTAG | 2 cut(s) | 77, 224 |
| BfmI | CTRYAG | 2 cut(s) | 629, 954 |
| BfoI | RGCGCY | 4 cut(s) | 77, 91, 209, 870 |
| BfuAI | ACCTGC | 1 cut(s) | 947 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 170 |
| Bme1390I | CCNGG | 5 cut(s) | 424, 835, 836, 1058, 1073 |
| Bme18I | GGWCC | 3 cut(s) | 51, 1005, 1116 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1098 |
| BmeT110I | CYCGRG | 2 cut(s) | 834, 967 |
| BmgT120I | GGNCC | 9 cut(s) | 51, 144, 145, 163, 831, 832, 986, 1005, 1116 |
| BmrFI | CCNGG | 5 cut(s) | 424, 835, 836, 1058, 1073 |
| BmrI | ACTGGG | 1 cut(s) | 1092 |
| BmuI | ACTGGG | 1 cut(s) | 1092 |
| BoxI | GACNNNNGTC | 1 cut(s) | 935 |
| BpiI | GAAGAC | 2 cut(s) | 188, 809 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 212, 244 |
| BpmI | CTGGAG | 2 cut(s) | 446, 1079 |
| Bpu10I | CCTNAGC | 1 cut(s) | 440 |
| Bpu14I | TTCGAA | 1 cut(s) | 179 |
| BpuMI | CCSGG | 3 cut(s) | 424, 835, 836 |
| BsaAI | YACGTR | 1 cut(s) | 1046 |
| BsaHI | GRCGYC | 4 cut(s) | 206, 410, 543, 867 |
| BsaI | GGTCTC | 2 cut(s) | 464, 1085 |
| BsaJI | CCNNGG | 5 cut(s) | 422, 547, 813, 834, 1071 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 114, 144, 257, 287 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 60, 430, 573, 956, 1001, 1036 |
| Bse118I | RCCGGY | 1 cut(s) | 907 |
| Bse1I | ACTGG | 3 cut(s) | 35, 498, 1098 |
| BseBI | CCWGG | 2 cut(s) | 1058, 1073 |
| BseDI | CCNNGG | 5 cut(s) | 422, 547, 813, 834, 1071 |
| BseGI | GGATG | 3 cut(s) | 699, 991, 1084 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 60, 430, 573, 956, 1001, 1036 |
| BseMII | CTCAG | 5 cut(s) | 90, 228, 454, 725, 903 |
| BseNI | ACTGG | 3 cut(s) | 35, 498, 1098 |
| BseSI | GKGCMC | 2 cut(s) | 148, 835 |
| BseX3I | CGGCCG | 1 cut(s) | 759 |
| BseXI | GCAGC | 6 cut(s) | 430, 615, 685, 742, 940, 984 |
| BseYI | CCCAGC | 6 cut(s) | 59, 298, 712, 827, 949, 1008 |
| BsgI | GTGCAG | 1 cut(s) | 692 |
| Bsh1236I | CGCG | 3 cut(s) | 549, 597, 1111 |
| Bsh1285I | CGRYCG | 1 cut(s) | 762 |
| BshFI | GGCC | 6 cut(s) | 146, 164, 566, 761, 833, 987 |
| BshNI | GGYRCC | 3 cut(s) | 205, 866, 1082 |
| BsiEI | CGRYCG | 1 cut(s) | 762 |
| BsiHKAI | GWGCWC | 1 cut(s) | 441 |
| BsiHKCI | CYCGRG | 2 cut(s) | 834, 967 |
| BsiSI | CCGG | 6 cut(s) | 91, 424, 835, 908, 926, 1050 |
| BslFI | GGGAC | 3 cut(s) | 590, 905, 991 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 60, 430, 573, 956, 1001, 1036 |
| BsmAI | GTCTC | 5 cut(s) | 108, 464, 604, 923, 1085 |
| BsmBI | CGTCTC | 1 cut(s) | 604 |
| BsmFI | GGGAC | 3 cut(s) | 590, 905, 991 |
| BsnI | GGCC | 6 cut(s) | 146, 164, 566, 761, 833, 987 |
| Bso31I | GGTCTC | 2 cut(s) | 464, 1085 |
| BsoBI | CYCGRG | 2 cut(s) | 834, 967 |
| Bsp119I | TTCGAA | 1 cut(s) | 179 |
| Bsp120I | GGGCCC | 2 cut(s) | 144, 831 |
| Bsp1286I | GDGCHC | 3 cut(s) | 148, 441, 835 |
| Bsp143I | GATC | 1 cut(s) | 618 |
| BspANI | GGCC | 6 cut(s) | 146, 164, 566, 761, 833, 987 |
| BspCNI | CTCAG | 5 cut(s) | 91, 229, 453, 726, 904 |
| BspFNI | CGCG | 3 cut(s) | 549, 597, 1111 |
| BspMAI | CTGCAG | 2 cut(s) | 633, 958 |
| BspMI | ACCTGC | 1 cut(s) | 947 |
| BspT104I | TTCGAA | 1 cut(s) | 179 |
| BspT107I | GGYRCC | 3 cut(s) | 205, 866, 1082 |
| BspTNI | GGTCTC | 2 cut(s) | 464, 1085 |
| BsrBI | CCGCTC | 4 cut(s) | 268, 295, 508, 637 |
| BsrFI | RCCGGY | 1 cut(s) | 907 |
| BsrI | ACTGG | 3 cut(s) | 35, 498, 1098 |
| BssAI | RCCGGY | 1 cut(s) | 907 |
| BssECI | CCNNGG | 5 cut(s) | 422, 547, 813, 834, 1071 |
| BssMI | GATC | 1 cut(s) | 618 |
| BssNI | GRCGYC | 4 cut(s) | 206, 410, 543, 867 |
| Bst2UI | CCWGG | 2 cut(s) | 1058, 1073 |
| Bst4CI | ACNGT | 3 cut(s) | 152, 214, 249 |
| Bst6I | CTCTTC | 2 cut(s) | 111, 284 |
| BstACI | GRCGYC | 4 cut(s) | 206, 410, 543, 867 |
| BstBAI | YACGTR | 1 cut(s) | 1046 |
| BstBI | TTCGAA | 1 cut(s) | 179 |
| BstC8I | GCNNGC | 5 cut(s) | 293, 547, 554, 909, 945 |
| BstDEI | CTNAG | 5 cut(s) | 99, 237, 440, 734, 912 |
| BstDSI | CCRYGG | 1 cut(s) | 547 |
| BstF5I | GGATG | 3 cut(s) | 699, 991, 1084 |
| BstFNI | CGCG | 3 cut(s) | 549, 597, 1111 |
| BstH2I | RGCGCY | 4 cut(s) | 77, 91, 209, 870 |
| BstHHI | GCGC | 6 cut(s) | 76, 90, 208, 869, 1066, 1111 |
| BstKTI | GATC | 1 cut(s) | 621 |
| BstMAI | GTCTC | 5 cut(s) | 108, 464, 604, 923, 1085 |
| BstMBI | GATC | 1 cut(s) | 618 |
| BstMCI | CGRYCG | 1 cut(s) | 762 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 170, 242, 634, 761, 764, 773, 824, 953 |
| BstNI | CCWGG | 2 cut(s) | 1058, 1073 |
| BstNSI | RCATGY | 1 cut(s) | 853 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 935 |
| BstSCI | CCNGG | 5 cut(s) | 422, 833, 834, 1056, 1071 |
| BstSFI | CTRYAG | 2 cut(s) | 629, 954 |
| BstSLI | GKGCMC | 2 cut(s) | 148, 835 |
| BstUI | CGCG | 3 cut(s) | 549, 597, 1111 |
| BstV1I | GCAGC | 6 cut(s) | 430, 615, 685, 742, 940, 984 |
| BstV2I | GAAGAC | 2 cut(s) | 188, 809 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1079 |
| BstZI | CGGCCG | 1 cut(s) | 759 |
| BsuRI | GGCC | 6 cut(s) | 146, 164, 566, 761, 833, 987 |
| BtgI | CCRYGG | 1 cut(s) | 547 |
| BtsCI | GGATG | 3 cut(s) | 699, 991, 1084 |
| BtsI | GCAGTG | 1 cut(s) | 623 |
| BtsIMutI | CAGTG | 4 cut(s) | 42, 223, 623, 806 |
| BveI | ACCTGC | 1 cut(s) | 947 |
| Cac8I | GCNNGC | 5 cut(s) | 293, 547, 554, 909, 945 |
| CciNI | GCGGCCGC | 1 cut(s) | 759 |
| CfoI | GCGC | 6 cut(s) | 76, 90, 208, 869, 1066, 1111 |
| Cfr10I | RCCGGY | 1 cut(s) | 907 |
| Cfr13I | GGNCC | 9 cut(s) | 51, 144, 145, 163, 831, 832, 986, 1005, 1116 |
| Cfr42I | CCGCGG | 1 cut(s) | 550 |
| Cfr9I | CCCGGG | 1 cut(s) | 834 |
| CseI | GACGC | 3 cut(s) | 399, 551, 586 |
| Csp6I | GTAC | 4 cut(s) | 750, 902, 960, 1083 |
| CviAII | CATG | 2 cut(s) | 392, 850 |
| CviQI | GTAC | 4 cut(s) | 750, 902, 960, 1083 |
| DdeI | CTNAG | 5 cut(s) | 99, 237, 440, 734, 912 |
| DinI | GGCGCC | 2 cut(s) | 207, 868 |
| DpnI | GATC | 1 cut(s) | 620 |
| DpnII | GATC | 1 cut(s) | 618 |
| DrdI | GACNNNNNNGTC | 3 cut(s) | 100, 695, 1003 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1098 |
| DseDI | GACNNNNNNGTC | 3 cut(s) | 100, 695, 1003 |
| EaeI | YGGCCR | 2 cut(s) | 564, 759 |
| EagI | CGGCCG | 1 cut(s) | 759 |
| Eam1104I | CTCTTC | 2 cut(s) | 111, 284 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1098 |
| EarI | CTCTTC | 2 cut(s) | 111, 284 |
| EciI | GGCGGA | 4 cut(s) | 491, 512, 539, 1091 |
| Ecl136II | GAGCTC | 1 cut(s) | 439 |
| EclXI | CGGCCG | 1 cut(s) | 759 |
| Eco24I | GRGCYC | 3 cut(s) | 148, 441, 835 |
| Eco31I | GGTCTC | 2 cut(s) | 464, 1085 |
| Eco47I | GGWCC | 3 cut(s) | 51, 1005, 1116 |
| Eco52I | CGGCCG | 1 cut(s) | 759 |
| Eco53kI | GAGCTC | 1 cut(s) | 439 |
| Eco57I | CTGAAG | 2 cut(s) | 340, 762 |
| Eco72I | CACGTG | 1 cut(s) | 1046 |
| Eco88I | CYCGRG | 2 cut(s) | 834, 967 |
| EcoICRI | GAGCTC | 1 cut(s) | 439 |
| EcoO109I | RGGNCCY | 3 cut(s) | 144, 831, 1005 |
| EcoRII | CCWGG | 2 cut(s) | 1056, 1071 |
| EcoT38I | GRGCYC | 3 cut(s) | 148, 441, 835 |
| EgeI | GGCGCC | 2 cut(s) | 207, 868 |
| EheI | GGCGCC | 2 cut(s) | 207, 868 |
| Esp3I | CGTCTC | 1 cut(s) | 604 |
| FaeI | CATG | 2 cut(s) | 395, 853 |
| FaqI | GGGAC | 3 cut(s) | 590, 905, 991 |
| FatI | CATG | 2 cut(s) | 391, 849 |
| FblI | GTMKAC | 1 cut(s) | 683 |
| FokI | GGATG | 3 cut(s) | 706, 978, 1091 |
| FriOI | GRGCYC | 3 cut(s) | 148, 441, 835 |
| FspBI | CTAG | 2 cut(s) | 77, 224 |
| GlaI | GCGC | 6 cut(s) | 75, 89, 207, 868, 1065, 1110 |
| GsaI | CCCAGC | 6 cut(s) | 63, 302, 716, 831, 953, 1012 |
| GsuI | CTGGAG | 2 cut(s) | 446, 1079 |
| HaeII | RGCGCY | 4 cut(s) | 77, 91, 209, 870 |
| HaeIII | GGCC | 6 cut(s) | 146, 164, 566, 761, 833, 987 |
| HapII | CCGG | 6 cut(s) | 91, 424, 835, 908, 926, 1050 |
| HgaI | GACGC | 3 cut(s) | 399, 551, 586 |
| HhaI | GCGC | 6 cut(s) | 76, 90, 208, 869, 1066, 1111 |
| Hin1I | GRCGYC | 4 cut(s) | 206, 410, 543, 867 |
| Hin1II | CATG | 2 cut(s) | 395, 853 |
| Hin6I | GCGC | 6 cut(s) | 74, 88, 206, 867, 1064, 1109 |
| HinP1I | GCGC | 6 cut(s) | 74, 88, 206, 867, 1064, 1109 |
| HindIII | AAGCTT | 1 cut(s) | 554 |
| HinfI | GANTC | 5 cut(s) | 101, 227, 680, 1015, 1053 |
| HpaII | CCGG | 6 cut(s) | 91, 424, 835, 908, 926, 1050 |
| HphI | GGTGA | 2 cut(s) | 55, 731 |
| Hpy166II | GTNNAC | 1 cut(s) | 684 |
| Hpy188I | TCNGA | 6 cut(s) | 156, 455, 996, 1036, 1104, 1120 |
| Hpy188III | TCNNGA | 3 cut(s) | 107, 331, 463 |
| Hpy8I | GTNNAC | 1 cut(s) | 684 |
| Hpy99I | CGWCG | 4 cut(s) | 337, 415, 1000, 1036 |
| HpyAV | CCTTC | 8 cut(s) | 154, 186, 254, 364, 737, 817, 856, 995 |
| HpyCH4III | ACNGT | 3 cut(s) | 152, 214, 249 |
| HpyCH4IV | ACGT | 3 cut(s) | 900, 1045, 1167 |
| HpyCH4V | TGCA | 5 cut(s) | 245, 631, 673, 881, 956 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 170, 242, 634, 761, 764, 773, 824, 953 |
| HpyF3I | CTNAG | 5 cut(s) | 99, 237, 440, 734, 912 |
| HpySE526I | ACGT | 3 cut(s) | 900, 1045, 1167 |
| Hsp92I | GRCGYC | 4 cut(s) | 206, 410, 543, 867 |
| Hsp92II | CATG | 2 cut(s) | 395, 853 |
| HspAI | GCGC | 6 cut(s) | 74, 88, 206, 867, 1064, 1109 |
| KasI | GGCGCC | 2 cut(s) | 205, 866 |
| KflI | GGGWCCC | 1 cut(s) | 1005 |
| KpnI | GGTACC | 1 cut(s) | 1086 |
| KroI | GCCGGC | 1 cut(s) | 907 |
| KroNI | GCCGGC | 1 cut(s) | 909 |
| KspI | CCGCGG | 1 cut(s) | 550 |
| Kzo9I | GATC | 1 cut(s) | 618 |
| LmnI | GCTCC | 5 cut(s) | 178, 265, 333, 610, 634 |
| Lsp1109I | GCAGC | 6 cut(s) | 430, 615, 685, 742, 940, 984 |
| MaeI | CTAG | 2 cut(s) | 77, 224 |
| MaeII | ACGT | 3 cut(s) | 900, 1045, 1167 |
| MaeIII | GTNAC | 5 cut(s) | 16, 43, 724, 888, 1041 |
| MalI | GATC | 1 cut(s) | 620 |
| MbiI | CCGCTC | 4 cut(s) | 268, 295, 508, 637 |
| MboI | GATC | 1 cut(s) | 618 |
| MboII | GAAGA | 7 cut(s) | 17, 128, 169, 193, 271, 458, 814 |
| MfeI | CAATTG | 1 cut(s) | 339 |
| MhlI | GDGCHC | 3 cut(s) | 148, 441, 835 |
| MluCI | AATT | 1 cut(s) | 339 |
| MluI | ACGCGT | 1 cut(s) | 595 |
| Mly113I | GGCGCC | 2 cut(s) | 206, 867 |
| MlyI | GAGTC | 4 cut(s) | 110, 221, 689, 1009 |
| MmeI | TCCRAC | 5 cut(s) | 588, 1014, 1019, 1127, 1143 |
| MroNI | GCCGGC | 1 cut(s) | 907 |
| MseI | TTAA | 2 cut(s) | 558, 798 |
| MslI | CAYNNNNRTG | 1 cut(s) | 668 |
| MspA1I | CMGCKG | 7 cut(s) | 59, 549, 676, 755, 767, 953, 975 |
| MspI | CCGG | 6 cut(s) | 91, 424, 835, 908, 926, 1050 |
| MspR9I | CCNGG | 5 cut(s) | 424, 835, 836, 1058, 1073 |
| MunI | CAATTG | 1 cut(s) | 339 |
| MvaI | CCWGG | 2 cut(s) | 1058, 1073 |
| MvnI | CGCG | 3 cut(s) | 549, 597, 1111 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 170, 242, 634, 761, 764, 773, 824, 953 |
| NaeI | GCCGGC | 1 cut(s) | 909 |
| NarI | GGCGCC | 2 cut(s) | 206, 867 |
| NciI | CCSGG | 3 cut(s) | 424, 835, 836 |
| NdeII | GATC | 1 cut(s) | 618 |
| NgoMIV | GCCGGC | 1 cut(s) | 907 |
| NlaIII | CATG | 2 cut(s) | 395, 853 |
| NmeAIII | GCCGAG | 1 cut(s) | 341 |
| NmuCI | GTSAC | 4 cut(s) | 16, 43, 888, 1041 |
| NotI | GCGGCCGC | 1 cut(s) | 759 |
| NspI | RCATGY | 1 cut(s) | 853 |
| NspV | TTCGAA | 1 cut(s) | 179 |
| PciI | ACATGT | 1 cut(s) | 849 |
| PdiI | GCCGGC | 1 cut(s) | 909 |
| PfeI | GAWTC | 1 cut(s) | 1053 |
| PflFI | GACNNNGTC | 1 cut(s) | 1030 |
| PfoI | TCCNGGA | 1 cut(s) | 1056 |
| PleI | GAGTC | 4 cut(s) | 109, 221, 688, 1009 |
| PluTI | GGCGCC | 2 cut(s) | 209, 870 |
| PmaCI | CACGTG | 1 cut(s) | 1046 |
| PmlI | CACGTG | 1 cut(s) | 1046 |
| PpsI | GAGTC | 4 cut(s) | 109, 221, 688, 1009 |
| Ppu21I | YACGTR | 1 cut(s) | 1046 |
| PpuMI | RGGWCCY | 1 cut(s) | 1005 |
| PscI | ACATGT | 1 cut(s) | 849 |
| PshAI | GACNNNNGTC | 1 cut(s) | 935 |
| Psp124BI | GAGCTC | 1 cut(s) | 441 |
| Psp5II | RGGWCCY | 1 cut(s) | 1005 |
| Psp6I | CCWGG | 2 cut(s) | 1056, 1071 |
| PspCI | CACGTG | 1 cut(s) | 1046 |
| PspFI | CCCAGC | 6 cut(s) | 59, 298, 712, 827, 949, 1008 |
| PspGI | CCWGG | 2 cut(s) | 1056, 1071 |
| PspOMI | GGGCCC | 2 cut(s) | 144, 831 |
| PspPI | GGNCC | 9 cut(s) | 51, 144, 145, 163, 831, 832, 986, 1005, 1116 |
| PspPPI | RGGWCCY | 1 cut(s) | 1005 |
| PstI | CTGCAG | 2 cut(s) | 633, 958 |
| PsyI | GACNNNGTC | 1 cut(s) | 1030 |
| PvuII | CAGCTG | 1 cut(s) | 953 |
| RsaI | GTAC | 4 cut(s) | 751, 903, 961, 1084 |
| RsaNI | GTAC | 4 cut(s) | 750, 902, 960, 1083 |
| RseI | CAYNNNNRTG | 1 cut(s) | 668 |
| SacI | GAGCTC | 1 cut(s) | 441 |
| SacII | CCGCGG | 1 cut(s) | 550 |
| SaqAI | TTAA | 2 cut(s) | 558, 798 |
| Sau3AI | GATC | 1 cut(s) | 618 |
| Sau96I | GGNCC | 9 cut(s) | 51, 144, 145, 163, 831, 832, 986, 1005, 1116 |
| SchI | GAGTC | 4 cut(s) | 110, 221, 689, 1009 |
| ScrFI | CCNGG | 5 cut(s) | 424, 835, 836, 1058, 1073 |
| SduI | GDGCHC | 3 cut(s) | 148, 441, 835 |
| SfcI | CTRYAG | 2 cut(s) | 629, 954 |
| SfoI | GGCGCC | 2 cut(s) | 207, 868 |
| Sfr303I | CCGCGG | 1 cut(s) | 550 |
| SfuI | TTCGAA | 1 cut(s) | 179 |
| SgrBI | CCGCGG | 1 cut(s) | 550 |
| SinI | GGWCC | 3 cut(s) | 51, 1005, 1116 |
| SmaI | CCCGGG | 1 cut(s) | 836 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 668 |
| SrfI | GCCCGGGC | 1 cut(s) | 836 |
| Sse9I | AATT | 1 cut(s) | 339 |
| SspDI | GGCGCC | 2 cut(s) | 205, 866 |
| SspMI | CTAG | 2 cut(s) | 77, 224 |
| SstI | GAGCTC | 1 cut(s) | 441 |
| StyD4I | CCNGG | 5 cut(s) | 422, 833, 834, 1056, 1071 |
| TaaI | ACNGT | 3 cut(s) | 152, 214, 249 |
| TaiI | ACGT | 3 cut(s) | 903, 1048, 1170 |
| TaqI | TCGA | 4 cut(s) | 179, 413, 504, 699 |
| TaqII | GACCGA | 1 cut(s) | 827 |
| TasI | AATT | 1 cut(s) | 339 |
| TfiI | GAWTC | 1 cut(s) | 1053 |
| Tru1I | TTAA | 2 cut(s) | 558, 798 |
| Tru9I | TTAA | 2 cut(s) | 558, 798 |
| TscAI | CASTG | 4 cut(s) | 42, 223, 630, 813 |
| TseFI | GTSAC | 4 cut(s) | 16, 43, 888, 1041 |
| TseI | GCWGC | 6 cut(s) | 418, 628, 673, 755, 953, 972 |
| Tsp45I | GTSAC | 4 cut(s) | 16, 43, 888, 1041 |
| TspDTI | ATGAA | 2 cut(s) | 606, 719 |
| TspGWI | ACGGA | 1 cut(s) | 1013 |
| TspMI | CCCGGG | 1 cut(s) | 834 |
| TspRI | CASTG | 4 cut(s) | 42, 223, 630, 813 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1030 |
| VpaK11BI | GGWCC | 3 cut(s) | 51, 1005, 1116 |
| XceI | RCATGY | 1 cut(s) | 853 |
| XmaI | CCCGGG | 1 cut(s) | 834 |
| XmiI | GTMKAC | 1 cut(s) | 683 |
| XspI | CTAG | 2 cut(s) | 77, 224 |