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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1051 |
| Acc65I | GGTACC | 1 cut(s) | 281 |
| AccB1I | GGYRCC | 5 cut(s) | 281, 311, 949, 1736, 1832 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 517, 564, 1746 |
| AccBSI | CCGCTC | 5 cut(s) | 220, 1303, 1444, 1620, 1839 |
| AccII | CGCG | 6 cut(s) | 443, 477, 788, 822, 1063, 1980 |
| AccIII | TCCGGA | 1 cut(s) | 195 |
| AclWI | GGATC | 1 cut(s) | 1531 |
| AcoI | YGGCCR | 6 cut(s) | 972, 1200, 1428, 1512, 1566, 1707 |
| AcsI | RAATTY | 3 cut(s) | 123, 513, 586 |
| AcuI | CTGAAG | 5 cut(s) | 276, 386, 1055, 1316, 1526 |
| AcyI | GRCGYC | 4 cut(s) | 200, 798, 1737, 2019 |
| AfaI | GTAC | 5 cut(s) | 283, 659, 854, 1258, 1951 |
| AflIII | ACRYGT | 2 cut(s) | 10, 561 |
| AgeI | ACCGGT | 1 cut(s) | 929 |
| AgsI | TTSAA | 2 cut(s) | 343, 2047 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1965 |
| AjnI | CCWGG | 8 cut(s) | 303, 669, 840, 1353, 1449, 1602, 1704, 1821 |
| AleI | CACNNNNGTG | 2 cut(s) | 1007, 1064 |
| AluBI | AGCT | 3 cut(s) | 1209, 1426, 2067 |
| AluI | AGCT | 3 cut(s) | 1209, 1426, 2067 |
| Alw21I | GWGCWC | 2 cut(s) | 462, 993 |
| Alw26I | GTCTC | 5 cut(s) | 68, 761, 890, 1952, 2025 |
| Alw44I | GTGCAC | 2 cut(s) | 458, 989 |
| AlwI | GGATC | 1 cut(s) | 1531 |
| Ama87I | CYCGRG | 6 cut(s) | 1169, 1293, 1840, 1867, 1938, 1963 |
| Aor13HI | TCCGGA | 1 cut(s) | 195 |
| ApaI | GGGCCC | 2 cut(s) | 56, 1138 |
| ApaLI | GTGCAC | 2 cut(s) | 458, 989 |
| ApoI | RAATTY | 3 cut(s) | 123, 513, 586 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1069, 1101 |
| AsiGI | ACCGGT | 1 cut(s) | 929 |
| Asp718I | GGTACC | 1 cut(s) | 281 |
| AspLEI | GCGC | 9 cut(s) | 583, 824, 907, 970, 984, 1625, 1678, 1739, 1862 |
| AsuHPI | GGTGA | 5 cut(s) | 406, 496, 898, 994, 1601 |
| AvaI | CYCGRG | 6 cut(s) | 1169, 1293, 1840, 1867, 1938, 1963 |
| AvaII | GGWCC | 8 cut(s) | 111, 177, 193, 298, 554, 617, 817, 1447 |
| BaeGI | GKGCMC | 5 cut(s) | 56, 462, 993, 1138, 1837 |
| BalI | TGGCCA | 1 cut(s) | 1709 |
| BanI | GGYRCC | 5 cut(s) | 281, 311, 949, 1736, 1832 |
| BanII | GRGCYC | 3 cut(s) | 56, 1037, 1138 |
| BauI | CACGAG | 4 cut(s) | 461, 756, 1204, 1416 |
| BbsI | GAAGAC | 2 cut(s) | 74, 707 |
| Bbv12I | GWGCWC | 2 cut(s) | 462, 993 |
| BbvCI | CCTCAGC | 1 cut(s) | 1611 |
| BccI | CCATC | 4 cut(s) | 302, 1244, 1823, 1940 |
| BceAI | ACGGC | 6 cut(s) | 338, 440, 777, 1103, 1415, 1595 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 82, 116, 674, 708, 1160, 1194 |
| BciT130I | CCWGG | 8 cut(s) | 305, 671, 842, 1355, 1451, 1604, 1706, 1823 |
| BciVI | GTATCC | 1 cut(s) | 1780 |
| BcoDI | GTCTC | 5 cut(s) | 68, 761, 890, 1952, 2025 |
| BfaI | CTAG | 4 cut(s) | 210, 497, 1214, 1560 |
| BfmI | CTRYAG | 1 cut(s) | 1157 |
| BfoI | RGCGCY | 7 cut(s) | 584, 908, 971, 1626, 1679, 1740, 1863 |
| BfuAI | ACCTGC | 1 cut(s) | 1051 |
| BfuI | GTATCC | 1 cut(s) | 1780 |
| BlpI | GCTNAGC | 1 cut(s) | 908 |
| Bme18I | GGWCC | 8 cut(s) | 111, 177, 193, 298, 554, 617, 817, 1447 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1965 |
| BmeT110I | CYCGRG | 6 cut(s) | 1169, 1293, 1840, 1867, 1938, 1963 |
| BmrI | ACTGGG | 1 cut(s) | 1756 |
| BmsI | GCATC | 5 cut(s) | 564, 1105, 1402, 1690, 1766 |
| BmuI | ACTGGG | 1 cut(s) | 1756 |
| BpiI | GAAGAC | 2 cut(s) | 74, 707 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 413, 445 |
| BpmI | CTGGAG | 4 cut(s) | 287, 1021, 1124, 1604 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1611 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 908 |
| BsaAI | YACGTR | 1 cut(s) | 661 |
| BsaHI | GRCGYC | 4 cut(s) | 200, 798, 1737, 2019 |
| BsaI | GGTCTC | 3 cut(s) | 68, 1952, 2025 |
| BsaWI | WCCGGW | 3 cut(s) | 195, 378, 929 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 255, 285 |
| Bse118I | RCCGGY | 4 cut(s) | 360, 929, 970, 1765 |
| Bse1I | ACTGG | 4 cut(s) | 243, 657, 727, 1751 |
| BseAI | TCCGGA | 1 cut(s) | 195 |
| BseBI | CCWGG | 8 cut(s) | 305, 671, 842, 1355, 1451, 1604, 1706, 1823 |
| BseGI | GGATG | 5 cut(s) | 313, 1120, 1757, 1879, 1951 |
| BseMII | CTCAG | 3 cut(s) | 909, 1602, 1910 |
| BseNI | ACTGG | 4 cut(s) | 243, 657, 727, 1751 |
| BseRI | GAGGAG | 4 cut(s) | 230, 434, 1124, 1331 |
| BseSI | GKGCMC | 5 cut(s) | 56, 462, 993, 1138, 1837 |
| BseYI | CCCAGC | 4 cut(s) | 203, 726, 1881, 1916 |
| BsgI | GTGCAG | 2 cut(s) | 258, 623 |
| Bsh1236I | CGCG | 6 cut(s) | 443, 477, 788, 822, 1063, 1980 |
| Bsh1285I | CGRYCG | 4 cut(s) | 741, 930, 1632, 1770 |
| BshNI | GGYRCC | 5 cut(s) | 281, 311, 949, 1736, 1832 |
| BshTI | ACCGGT | 1 cut(s) | 929 |
| BsiEI | CGRYCG | 4 cut(s) | 741, 930, 1632, 1770 |
| BsiHKAI | GWGCWC | 2 cut(s) | 462, 993 |
| BsiHKCI | CYCGRG | 6 cut(s) | 1169, 1293, 1840, 1867, 1938, 1963 |
| BslFI | GGGAC | 6 cut(s) | 540, 630, 1352, 1460, 1477, 1894 |
| BsmAI | GTCTC | 5 cut(s) | 68, 761, 890, 1952, 2025 |
| BsmBI | CGTCTC | 1 cut(s) | 890 |
| BsmFI | GGGAC | 6 cut(s) | 540, 630, 1352, 1460, 1477, 1894 |
| BsmI | GAATGC | 1 cut(s) | 93 |
| Bso31I | GGTCTC | 3 cut(s) | 68, 1952, 2025 |
| BsoBI | CYCGRG | 6 cut(s) | 1169, 1293, 1840, 1867, 1938, 1963 |
| Bsp120I | GGGCCC | 2 cut(s) | 52, 1134 |
| Bsp1286I | GDGCHC | 6 cut(s) | 56, 462, 993, 1037, 1138, 1837 |
| Bsp13I | TCCGGA | 1 cut(s) | 195 |
| Bsp1407I | TGTACA | 1 cut(s) | 1256 |
| Bsp143I | GATC | 5 cut(s) | 432, 742, 1125, 1504, 1536 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 908 |
| Bsp19I | CCATGG | 1 cut(s) | 813 |
| Bsp68I | TCGCGA | 1 cut(s) | 477 |
| BspCNI | CTCAG | 3 cut(s) | 910, 1603, 1909 |
| BspEI | TCCGGA | 1 cut(s) | 195 |
| BspFNI | CGCG | 6 cut(s) | 443, 477, 788, 822, 1063, 1980 |
| BspMI | ACCTGC | 1 cut(s) | 1051 |
| BspPI | GGATC | 1 cut(s) | 1531 |
| BspT107I | GGYRCC | 5 cut(s) | 281, 311, 949, 1736, 1832 |
| BspTNI | GGTCTC | 3 cut(s) | 68, 1952, 2025 |
| BsrBI | CCGCTC | 5 cut(s) | 220, 1303, 1444, 1620, 1839 |
| BsrFI | RCCGGY | 4 cut(s) | 360, 929, 970, 1765 |
| BsrGI | TGTACA | 1 cut(s) | 1256 |
| BsrI | ACTGG | 4 cut(s) | 243, 657, 727, 1751 |
| BssAI | RCCGGY | 4 cut(s) | 360, 929, 970, 1765 |
| BssMI | GATC | 5 cut(s) | 432, 742, 1125, 1504, 1536 |
| BssNI | GRCGYC | 4 cut(s) | 200, 798, 1737, 2019 |
| BssSI | CACGAG | 4 cut(s) | 461, 756, 1204, 1416 |
| BssT1I | CCWWGG | 2 cut(s) | 813, 1782 |
| Bst2BI | CACGAG | 4 cut(s) | 461, 756, 1204, 1416 |
| Bst2UI | CCWGG | 8 cut(s) | 305, 671, 842, 1355, 1451, 1604, 1706, 1823 |
| Bst4CI | ACNGT | 4 cut(s) | 415, 625, 883, 1006 |
| Bst6I | CTCTTC | 1 cut(s) | 1101 |
| BstACI | GRCGYC | 4 cut(s) | 200, 798, 1737, 2019 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1412 |
| BstAUI | TGTACA | 1 cut(s) | 1256 |
| BstBAI | YACGTR | 1 cut(s) | 661 |
| BstDEI | CTNAG | 4 cut(s) | 908, 918, 1611, 1896 |
| BstDSI | CCRYGG | 4 cut(s) | 441, 813, 1119, 1978 |
| BstEII | GGTNACC | 1 cut(s) | 484 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1388 |
| BstF5I | GGATG | 5 cut(s) | 313, 1120, 1757, 1879, 1951 |
| BstFNI | CGCG | 6 cut(s) | 443, 477, 788, 822, 1063, 1980 |
| BstH2I | RGCGCY | 7 cut(s) | 584, 908, 971, 1626, 1679, 1740, 1863 |
| BstHHI | GCGC | 9 cut(s) | 583, 824, 907, 970, 984, 1625, 1678, 1739, 1862 |
| BstKTI | GATC | 5 cut(s) | 435, 745, 1128, 1507, 1539 |
| BstMAI | GTCTC | 5 cut(s) | 68, 761, 890, 1952, 2025 |
| BstMBI | GATC | 5 cut(s) | 432, 742, 1125, 1504, 1536 |
| BstMCI | CGRYCG | 4 cut(s) | 741, 930, 1632, 1770 |
| BstNI | CCWGG | 8 cut(s) | 305, 671, 842, 1355, 1451, 1604, 1706, 1823 |
| BstNSI | RCATGY | 2 cut(s) | 14, 565 |
| BstPI | GGTNACC | 1 cut(s) | 484 |
| BstSFI | CTRYAG | 1 cut(s) | 1157 |
| BstSLI | GKGCMC | 5 cut(s) | 56, 462, 993, 1138, 1837 |
| BstUI | CGCG | 6 cut(s) | 443, 477, 788, 822, 1063, 1980 |
| BstV2I | GAAGAC | 2 cut(s) | 74, 707 |
| BstX2I | RGATCY | 1 cut(s) | 1536 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1829 |
| BstYI | RGATCY | 1 cut(s) | 1536 |
| BsuI | GTATCC | 1 cut(s) | 1780 |
| BtgI | CCRYGG | 4 cut(s) | 441, 813, 1119, 1978 |
| BtsCI | GGATG | 5 cut(s) | 313, 1120, 1757, 1879, 1951 |
| BtsIMutI | CAGTG | 3 cut(s) | 250, 420, 888 |
| BtuMI | TCGCGA | 1 cut(s) | 477 |
| BveI | ACCTGC | 1 cut(s) | 1051 |
| CfoI | GCGC | 9 cut(s) | 583, 824, 907, 970, 984, 1625, 1678, 1739, 1862 |
| Cfr10I | RCCGGY | 4 cut(s) | 360, 929, 970, 1765 |
| Cfr42I | CCGCGG | 2 cut(s) | 444, 1981 |
| Cfr9I | CCCGGG | 3 cut(s) | 1293, 1867, 1938 |
| CpoI | CGGWCCG | 1 cut(s) | 111 |
| CseI | GACGC | 6 cut(s) | 208, 392, 806, 1367, 1412, 2027 |
| Csp6I | GTAC | 5 cut(s) | 282, 658, 853, 1257, 1950 |
| CspAI | ACCGGT | 1 cut(s) | 929 |
| CspI | CGGWCCG | 1 cut(s) | 111 |
| CviAII | CATG | 6 cut(s) | 11, 43, 562, 814, 850, 1762 |
| CviQI | GTAC | 5 cut(s) | 282, 658, 853, 1257, 1950 |
| DdeI | CTNAG | 4 cut(s) | 908, 918, 1611, 1896 |
| DinI | GGCGCC | 1 cut(s) | 1738 |
| DpnI | GATC | 5 cut(s) | 434, 744, 1127, 1506, 1538 |
| DpnII | GATC | 5 cut(s) | 432, 742, 1125, 1504, 1536 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1965 |
| EaeI | YGGCCR | 6 cut(s) | 972, 1200, 1428, 1512, 1566, 1707 |
| Eam1104I | CTCTTC | 1 cut(s) | 1101 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1965 |
| EarI | CTCTTC | 1 cut(s) | 1101 |
| EciI | GGCGGA | 7 cut(s) | 396, 644, 762, 1116, 1155, 1676, 1958 |
| Eco130I | CCWWGG | 2 cut(s) | 813, 1782 |
| Eco147I | AGGCCT | 1 cut(s) | 428 |
| Eco24I | GRGCYC | 3 cut(s) | 56, 1037, 1138 |
| Eco31I | GGTCTC | 3 cut(s) | 68, 1952, 2025 |
| Eco47I | GGWCC | 8 cut(s) | 111, 177, 193, 298, 554, 617, 817, 1447 |
| Eco57I | CTGAAG | 5 cut(s) | 276, 386, 1055, 1316, 1526 |
| Eco88I | CYCGRG | 6 cut(s) | 1169, 1293, 1840, 1867, 1938, 1963 |
| Eco91I | GGTNACC | 1 cut(s) | 484 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1388 |
| EcoO109I | RGGNCCY | 8 cut(s) | 53, 177, 298, 617, 1134, 1135, 1447, 1814 |
| EcoO65I | GGTNACC | 1 cut(s) | 484 |
| EcoRI | GAATTC | 1 cut(s) | 586 |
| EcoRII | CCWGG | 8 cut(s) | 303, 669, 840, 1353, 1449, 1602, 1704, 1821 |
| EcoT14I | CCWWGG | 2 cut(s) | 813, 1782 |
| EcoT22I | ATGCAT | 1 cut(s) | 95 |
| EcoT38I | GRGCYC | 3 cut(s) | 56, 1037, 1138 |
| EgeI | GGCGCC | 1 cut(s) | 1738 |
| EheI | GGCGCC | 1 cut(s) | 1738 |
| ErhI | CCWWGG | 2 cut(s) | 813, 1782 |
| Esp3I | CGTCTC | 1 cut(s) | 890 |
| FaeI | CATG | 6 cut(s) | 14, 46, 565, 817, 853, 1765 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1846, 1878 |
| FaqI | GGGAC | 6 cut(s) | 540, 630, 1352, 1460, 1477, 1894 |
| FatI | CATG | 6 cut(s) | 10, 42, 561, 813, 849, 1761 |
| FauNDI | CATATG | 2 cut(s) | 19, 1231 |
| FokI | GGATG | 5 cut(s) | 320, 1127, 1744, 1886, 1958 |
| FriOI | GRGCYC | 3 cut(s) | 56, 1037, 1138 |
| FspBI | CTAG | 4 cut(s) | 210, 497, 1214, 1560 |
| GlaI | GCGC | 9 cut(s) | 582, 823, 906, 969, 983, 1624, 1677, 1738, 1861 |
| GsaI | CCCAGC | 4 cut(s) | 207, 730, 1885, 1920 |
| GsuI | CTGGAG | 4 cut(s) | 287, 1021, 1124, 1604 |
| HaeII | RGCGCY | 7 cut(s) | 584, 908, 971, 1626, 1679, 1740, 1863 |
| HgaI | GACGC | 6 cut(s) | 208, 392, 806, 1367, 1412, 2027 |
| HhaI | GCGC | 9 cut(s) | 583, 824, 907, 970, 984, 1625, 1678, 1739, 1862 |
| Hin1I | GRCGYC | 4 cut(s) | 200, 798, 1737, 2019 |
| Hin1II | CATG | 6 cut(s) | 14, 46, 565, 817, 853, 1765 |
| Hin6I | GCGC | 9 cut(s) | 581, 822, 905, 968, 982, 1623, 1676, 1737, 1860 |
| HinP1I | GCGC | 9 cut(s) | 581, 822, 905, 968, 982, 1623, 1676, 1737, 1860 |
| HincII | GTYRAC | 1 cut(s) | 358 |
| HindII | GTYRAC | 1 cut(s) | 358 |
| HphI | GGTGA | 5 cut(s) | 406, 496, 898, 994, 1601 |
| Hpy166II | GTNNAC | 5 cut(s) | 358, 460, 658, 889, 991 |
| Hpy188I | TCNGA | 5 cut(s) | 111, 919, 1089, 1335, 1903 |
| Hpy188III | TCNNGA | 3 cut(s) | 196, 476, 1038 |
| Hpy8I | GTNNAC | 5 cut(s) | 358, 460, 658, 889, 991 |
| Hpy99I | CGWCG | 2 cut(s) | 1383, 1436 |
| HpyAV | CCTTC | 8 cut(s) | 251, 418, 1144, 1340, 1550, 1666, 1687, 2018 |
| HpyCH4III | ACNGT | 4 cut(s) | 415, 625, 883, 1006 |
| HpyCH4IV | ACGT | 2 cut(s) | 660, 899 |
| HpyCH4V | TGCA | 8 cut(s) | 93, 239, 460, 612, 640, 938, 991, 1726 |
| HpyF3I | CTNAG | 4 cut(s) | 908, 918, 1611, 1896 |
| HpySE526I | ACGT | 2 cut(s) | 660, 899 |
| Hsp92I | GRCGYC | 4 cut(s) | 200, 798, 1737, 2019 |
| Hsp92II | CATG | 6 cut(s) | 14, 46, 565, 817, 853, 1765 |
| HspAI | GCGC | 9 cut(s) | 581, 822, 905, 968, 982, 1623, 1676, 1737, 1860 |
| KasI | GGCGCC | 1 cut(s) | 1736 |
| Kpn2I | TCCGGA | 1 cut(s) | 195 |
| KpnI | GGTACC | 1 cut(s) | 285 |
| KroI | GCCGGC | 1 cut(s) | 970 |
| KroNI | GCCGGC | 1 cut(s) | 972 |
| KspI | CCGCGG | 2 cut(s) | 444, 1981 |
| Kzo9I | GATC | 5 cut(s) | 432, 742, 1125, 1504, 1536 |
| LmnI | GCTCC | 8 cut(s) | 4, 217, 1018, 1040, 1168, 1375, 1480, 1617 |
| LweI | GCATC | 5 cut(s) | 564, 1105, 1402, 1690, 1766 |
| MaeI | CTAG | 4 cut(s) | 210, 497, 1214, 1560 |
| MaeII | ACGT | 2 cut(s) | 660, 899 |
| MaeIII | GTNAC | 5 cut(s) | 484, 794, 1000, 1400, 2051 |
| MalI | GATC | 5 cut(s) | 434, 744, 1127, 1506, 1538 |
| MbiI | CCGCTC | 5 cut(s) | 220, 1303, 1444, 1620, 1839 |
| MboI | GATC | 5 cut(s) | 432, 742, 1125, 1504, 1536 |
| MboII | GAAGA | 3 cut(s) | 79, 707, 1118 |
| MfeI | CAATTG | 1 cut(s) | 1396 |
| MflI | RGATCY | 1 cut(s) | 1536 |
| MhlI | GDGCHC | 6 cut(s) | 56, 462, 993, 1037, 1138, 1837 |
| MlsI | TGGCCA | 1 cut(s) | 1709 |
| MluCI | AATT | 5 cut(s) | 123, 513, 586, 1396, 1466 |
| MluNI | TGGCCA | 1 cut(s) | 1709 |
| Mly113I | GGCGCC | 1 cut(s) | 1737 |
| MlyI | GAGTC | 8 cut(s) | 296, 763, 1088, 1211, 1652, 1871, 1882, 1975 |
| MmeI | TCCRAC | 3 cut(s) | 996, 1458, 1916 |
| Mox20I | TGGCCA | 1 cut(s) | 1709 |
| Mph1103I | ATGCAT | 1 cut(s) | 95 |
| MroI | TCCGGA | 1 cut(s) | 195 |
| MroNI | GCCGGC | 1 cut(s) | 970 |
| MscI | TGGCCA | 1 cut(s) | 1709 |
| MseI | TTAA | 1 cut(s) | 1575 |
| MslI | CAYNNNNRTG | 3 cut(s) | 996, 1007, 1064 |
| Msp20I | TGGCCA | 1 cut(s) | 1709 |
| MspA1I | CMGCKG | 8 cut(s) | 254, 443, 688, 1052, 1546, 1637, 1885, 1980 |
| MunI | CAATTG | 1 cut(s) | 1396 |
| Mva1269I | GAATGC | 1 cut(s) | 93 |
| MvaI | CCWGG | 8 cut(s) | 305, 671, 842, 1355, 1451, 1604, 1706, 1823 |
| MvnI | CGCG | 6 cut(s) | 443, 477, 788, 822, 1063, 1980 |
| NaeI | GCCGGC | 1 cut(s) | 972 |
| NarI | GGCGCC | 1 cut(s) | 1737 |
| NcoI | CCATGG | 1 cut(s) | 813 |
| NdeI | CATATG | 2 cut(s) | 19, 1231 |
| NdeII | GATC | 5 cut(s) | 432, 742, 1125, 1504, 1536 |
| NgoMIV | GCCGGC | 1 cut(s) | 970 |
| NlaIII | CATG | 6 cut(s) | 14, 46, 565, 817, 853, 1765 |
| NmeAIII | GCCGAG | 3 cut(s) | 1053, 1261, 1594 |
| NmuCI | GTSAC | 4 cut(s) | 484, 794, 1000, 1400 |
| NruI | TCGCGA | 1 cut(s) | 477 |
| NsiI | ATGCAT | 1 cut(s) | 95 |
| NspI | RCATGY | 2 cut(s) | 14, 565 |
| OliI | CACNNNNGTG | 2 cut(s) | 1007, 1064 |
| PceI | AGGCCT | 1 cut(s) | 428 |
| PciI | ACATGT | 2 cut(s) | 10, 561 |
| PctI | GAATGC | 1 cut(s) | 93 |
| PdiI | GCCGGC | 1 cut(s) | 972 |
| PfeI | GAWTC | 4 cut(s) | 147, 691, 845, 1550 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 517, 564, 1746 |
| PfoI | TCCNGGA | 2 cut(s) | 303, 840 |
| PinAI | ACCGGT | 1 cut(s) | 929 |
| PleI | GAGTC | 8 cut(s) | 295, 763, 1088, 1211, 1651, 1871, 1882, 1974 |
| PluTI | GGCGCC | 1 cut(s) | 1740 |
| PpsI | GAGTC | 8 cut(s) | 295, 763, 1088, 1211, 1651, 1871, 1882, 1974 |
| Ppu21I | YACGTR | 1 cut(s) | 661 |
| PpuMI | RGGWCCY | 4 cut(s) | 177, 298, 617, 1447 |
| PscI | ACATGT | 2 cut(s) | 10, 561 |
| Psp5II | RGGWCCY | 4 cut(s) | 177, 298, 617, 1447 |
| Psp6I | CCWGG | 8 cut(s) | 303, 669, 840, 1353, 1449, 1602, 1704, 1821 |
| PspEI | GGTNACC | 1 cut(s) | 484 |
| PspFI | CCCAGC | 4 cut(s) | 203, 726, 1881, 1916 |
| PspGI | CCWGG | 8 cut(s) | 303, 669, 840, 1353, 1449, 1602, 1704, 1821 |
| PspOMI | GGGCCC | 2 cut(s) | 52, 1134 |
| PspPPI | RGGWCCY | 4 cut(s) | 177, 298, 617, 1447 |
| PsuI | RGATCY | 1 cut(s) | 1536 |
| RruI | TCGCGA | 1 cut(s) | 477 |
| RsaI | GTAC | 5 cut(s) | 283, 659, 854, 1258, 1951 |
| RsaNI | GTAC | 5 cut(s) | 282, 658, 853, 1257, 1950 |
| RseI | CAYNNNNRTG | 3 cut(s) | 996, 1007, 1064 |
| Rsr2I | CGGWCCG | 1 cut(s) | 111 |
| RsrII | CGGWCCG | 1 cut(s) | 111 |
| SacII | CCGCGG | 2 cut(s) | 444, 1981 |
| SaqAI | TTAA | 1 cut(s) | 1575 |
| Sau3AI | GATC | 5 cut(s) | 432, 742, 1125, 1504, 1536 |
| SchI | GAGTC | 8 cut(s) | 296, 763, 1088, 1211, 1652, 1871, 1882, 1975 |
| SduI | GDGCHC | 6 cut(s) | 56, 462, 993, 1037, 1138, 1837 |
| SfaNI | GCATC | 5 cut(s) | 564, 1105, 1402, 1690, 1766 |
| SfcI | CTRYAG | 1 cut(s) | 1157 |
| SfoI | GGCGCC | 1 cut(s) | 1738 |
| Sfr303I | CCGCGG | 2 cut(s) | 444, 1981 |
| SgrBI | CCGCGG | 2 cut(s) | 444, 1981 |
| SinI | GGWCC | 8 cut(s) | 111, 177, 193, 298, 554, 617, 817, 1447 |
| SmaI | CCCGGG | 3 cut(s) | 1295, 1869, 1940 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 996, 1007, 1064 |
| Sse9I | AATT | 5 cut(s) | 123, 513, 586, 1396, 1466 |
| SseBI | AGGCCT | 1 cut(s) | 428 |
| SspDI | GGCGCC | 1 cut(s) | 1736 |
| SspMI | CTAG | 4 cut(s) | 210, 497, 1214, 1560 |
| StuI | AGGCCT | 1 cut(s) | 428 |
| StyI | CCWWGG | 2 cut(s) | 813, 1782 |
| TaaI | ACNGT | 4 cut(s) | 415, 625, 883, 1006 |
| TaiI | ACGT | 2 cut(s) | 663, 902 |
| TaqI | TCGA | 3 cut(s) | 431, 741, 1283 |
| TaqII | GACCGA | 7 cut(s) | 86, 92, 128, 172, 542, 727, 1697 |
| TasI | AATT | 5 cut(s) | 123, 513, 586, 1396, 1466 |
| TatI | WGTACW | 1 cut(s) | 1256 |
| TfiI | GAWTC | 4 cut(s) | 147, 691, 845, 1550 |
| Tru1I | TTAA | 1 cut(s) | 1575 |
| Tru9I | TTAA | 1 cut(s) | 1575 |
| TscAI | CASTG | 3 cut(s) | 250, 420, 888 |
| TseFI | GTSAC | 4 cut(s) | 484, 794, 1000, 1400 |
| Tsp45I | GTSAC | 4 cut(s) | 484, 794, 1000, 1400 |
| TspDTI | ATGAA | 4 cut(s) | 31, 80, 1476, 1782 |
| TspGWI | ACGGA | 1 cut(s) | 1471 |
| TspMI | CCCGGG | 3 cut(s) | 1293, 1867, 1938 |
| TspRI | CASTG | 3 cut(s) | 250, 420, 888 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 517, 564, 1746 |
| VneI | GTGCAC | 2 cut(s) | 458, 989 |
| VpaK11BI | GGWCC | 8 cut(s) | 111, 177, 193, 298, 554, 617, 817, 1447 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1388 |
| XapI | RAATTY | 3 cut(s) | 123, 513, 586 |
| XceI | RCATGY | 2 cut(s) | 14, 565 |
| XmaI | CCCGGG | 3 cut(s) | 1293, 1867, 1938 |
| XspI | CTAG | 4 cut(s) | 210, 497, 1214, 1560 |
| Zsp2I | ATGCAT | 1 cut(s) | 95 |