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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 124 |
| Acc36I | ACCTGC | 1 cut(s) | 124 |
| Acc65I | GGTACC | 1 cut(s) | 1700 |
| AccB1I | GGYRCC | 4 cut(s) | 827, 1128, 1700, 1757 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 327, 667 |
| AccBSI | CCGCTC | 5 cut(s) | 987, 1035, 1126, 1192, 1204 |
| AccI | GTMKAC | 2 cut(s) | 654, 1281 |
| AccII | CGCG | 6 cut(s) | 561, 588, 774, 899, 1167, 1409 |
| AclWI | GGATC | 4 cut(s) | 591, 756, 907, 920 |
| AcoI | YGGCCR | 5 cut(s) | 167, 960, 1263, 1532, 1691 |
| AcsI | RAATTY | 2 cut(s) | 276, 1016 |
| AcuI | CTGAAG | 6 cut(s) | 518, 830, 1079, 1343, 1493, 1674 |
| AcyI | GRCGYC | 6 cut(s) | 361, 476, 870, 1129, 1485, 1758 |
| AfaI | GTAC | 4 cut(s) | 399, 1522, 1702, 1772 |
| AflII | CTTAAG | 2 cut(s) | 128, 1079 |
| AflIII | ACRYGT | 1 cut(s) | 324 |
| AgsI | TTSAA | 4 cut(s) | 275, 1016, 1249, 1399 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1716 |
| AjnI | CCWGG | 3 cut(s) | 468, 1461, 1729 |
| AleI | CACNNNNGTG | 1 cut(s) | 836 |
| AluBI | AGCT | 7 cut(s) | 163, 484, 1111, 1174, 1241, 1321, 1643 |
| AluI | AGCT | 7 cut(s) | 163, 484, 1111, 1174, 1241, 1321, 1643 |
| Alw21I | GWGCWC | 1 cut(s) | 769 |
| Alw26I | GTCTC | 3 cut(s) | 680, 885, 1512 |
| Alw44I | GTGCAC | 1 cut(s) | 765 |
| AlwI | GGATC | 4 cut(s) | 591, 756, 907, 920 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 667, 1768 |
| Ama87I | CYCGRG | 3 cut(s) | 6, 725, 1662 |
| ApaI | GGGCCC | 1 cut(s) | 732 |
| ApaLI | GTGCAC | 1 cut(s) | 765 |
| ApeKI | GCWGC | 5 cut(s) | 47, 163, 441, 779, 1382 |
| ApoI | RAATTY | 2 cut(s) | 276, 1016 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 287, 319 |
| Asp718I | GGTACC | 1 cut(s) | 1700 |
| AspA2I | CCTAGG | 1 cut(s) | 149 |
| AspLEI | GCGC | 3 cut(s) | 590, 1131, 1760 |
| AspS9I | GGNCC | 9 cut(s) | 380, 637, 728, 729, 940, 1162, 1195, 1366, 1665 |
| AsuC2I | CCSGG | 4 cut(s) | 543, 1222, 1663, 1664 |
| AsuHPI | GGTGA | 5 cut(s) | 395, 685, 709, 964, 1444 |
| AsuNHI | GCTAGC | 1 cut(s) | 76 |
| AvaI | CYCGRG | 3 cut(s) | 6, 725, 1662 |
| AvaII | GGWCC | 2 cut(s) | 637, 1195 |
| AvrII | CCTAGG | 1 cut(s) | 149 |
| BaeGI | GKGCMC | 2 cut(s) | 732, 769 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1692, 1725 |
| BamHI | GGATCC | 1 cut(s) | 912 |
| BanI | GGYRCC | 4 cut(s) | 827, 1128, 1700, 1757 |
| BanII | GRGCYC | 1 cut(s) | 732 |
| BauI | CACGAG | 1 cut(s) | 416 |
| Bbv12I | GWGCWC | 1 cut(s) | 769 |
| BbvI | GCAGC | 5 cut(s) | 34, 150, 453, 766, 1369 |
| BccI | CCATC | 3 cut(s) | 334, 1616, 1691 |
| BceAI | ACGGC | 4 cut(s) | 101, 947, 1169, 1289 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 122, 156, 334, 368, 551, 585 |
| BciT130I | CCWGG | 3 cut(s) | 470, 1463, 1731 |
| BciVI | GTATCC | 1 cut(s) | 1228 |
| BcnI | CCSGG | 4 cut(s) | 543, 1222, 1663, 1664 |
| BcoDI | GTCTC | 3 cut(s) | 680, 885, 1512 |
| BfaI | CTAG | 8 cut(s) | 30, 77, 150, 992, 1199, 1572, 1640, 1746 |
| BfmI | CTRYAG | 1 cut(s) | 648 |
| BfoI | RGCGCY | 2 cut(s) | 1132, 1761 |
| BfrI | CTTAAG | 2 cut(s) | 128, 1079 |
| BfuAI | ACCTGC | 1 cut(s) | 124 |
| BfuI | GTATCC | 1 cut(s) | 1228 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1755 |
| BlnI | CCTAGG | 1 cut(s) | 149 |
| Bme1390I | CCNGG | 7 cut(s) | 470, 543, 1222, 1463, 1663, 1664, 1731 |
| Bme18I | GGWCC | 2 cut(s) | 637, 1195 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1716 |
| BmeT110I | CYCGRG | 3 cut(s) | 6, 725, 1662 |
| BmgT120I | GGNCC | 9 cut(s) | 380, 637, 728, 729, 940, 1162, 1195, 1366, 1665 |
| BmiI | GGNNCC | 8 cut(s) | 730, 829, 914, 942, 1130, 1196, 1702, 1759 |
| BmrFI | CCNGG | 7 cut(s) | 470, 543, 1222, 1463, 1663, 1664, 1731 |
| BmrI | ACTGGG | 2 cut(s) | 1361, 1504 |
| BmsI | GCATC | 7 cut(s) | 200, 553, 690, 696, 1300, 1567, 1671 |
| BmtI | GCTAGC | 1 cut(s) | 80 |
| BmuI | ACTGGG | 2 cut(s) | 1361, 1504 |
| BpmI | CTGGAG | 4 cut(s) | 675, 902, 1247, 1484 |
| Bpu10I | CCTNAGC | 1 cut(s) | 458 |
| BpuEI | CTTGAG | 2 cut(s) | 207, 829 |
| BpuMI | CCSGG | 4 cut(s) | 543, 1222, 1663, 1664 |
| Bsa29I | ATCGAT | 1 cut(s) | 194 |
| BsaAI | YACGTR | 1 cut(s) | 1216 |
| BsaHI | GRCGYC | 6 cut(s) | 361, 476, 870, 1129, 1485, 1758 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 602, 632, 1549, 1579 |
| Bse118I | RCCGGY | 2 cut(s) | 782, 824 |
| Bse1I | ACTGG | 7 cut(s) | 672, 679, 692, 766, 1356, 1499, 1518 |
| Bse3DI | GCAATG | 1 cut(s) | 1161 |
| BseBI | CCWGG | 3 cut(s) | 470, 1463, 1731 |
| BseCI | ATCGAT | 1 cut(s) | 194 |
| BseGI | GGATG | 3 cut(s) | 1284, 1624, 1702 |
| BseMI | GCAATG | 1 cut(s) | 1161 |
| BseMII | CTCAG | 3 cut(s) | 1422, 1523, 1706 |
| BseNI | ACTGG | 7 cut(s) | 672, 679, 692, 766, 1356, 1499, 1518 |
| BseRI | GAGGAG | 2 cut(s) | 899, 1626 |
| BseSI | GKGCMC | 2 cut(s) | 732, 769 |
| BseX3I | CGGCCG | 3 cut(s) | 167, 1532, 1691 |
| BseXI | GCAGC | 5 cut(s) | 34, 150, 453, 766, 1369 |
| BseYI | CCCAGC | 2 cut(s) | 626, 1761 |
| BsgI | GTGCAG | 1 cut(s) | 998 |
| Bsh1236I | CGCG | 6 cut(s) | 561, 588, 774, 899, 1167, 1409 |
| Bsh1285I | CGRYCG | 6 cut(s) | 61, 170, 387, 1281, 1535, 1694 |
| BshNI | GGYRCC | 4 cut(s) | 827, 1128, 1700, 1757 |
| BshVI | ATCGAT | 1 cut(s) | 194 |
| BsiEI | CGRYCG | 6 cut(s) | 61, 170, 387, 1281, 1535, 1694 |
| BsiHKAI | GWGCWC | 1 cut(s) | 769 |
| BsiHKCI | CYCGRG | 3 cut(s) | 6, 725, 1662 |
| BsiSI | CCGG | 7 cut(s) | 542, 783, 825, 1222, 1568, 1663, 1694 |
| BslFI | GGGAC | 5 cut(s) | 224, 303, 1208, 1643, 1704 |
| BsmAI | GTCTC | 3 cut(s) | 680, 885, 1512 |
| BsmBI | CGTCTC | 1 cut(s) | 885 |
| BsmFI | GGGAC | 5 cut(s) | 224, 303, 1208, 1643, 1704 |
| BsoBI | CYCGRG | 3 cut(s) | 6, 725, 1662 |
| Bsp120I | GGGCCC | 1 cut(s) | 728 |
| Bsp1286I | GDGCHC | 2 cut(s) | 732, 769 |
| Bsp143I | GATC | 5 cut(s) | 58, 231, 583, 748, 912 |
| Bsp19I | CCATGG | 1 cut(s) | 1386 |
| BspCNI | CTCAG | 3 cut(s) | 1423, 1522, 1707 |
| BspDI | ATCGAT | 1 cut(s) | 194 |
| BspFNI | CGCG | 6 cut(s) | 561, 588, 774, 899, 1167, 1409 |
| BspLI | GGNNCC | 8 cut(s) | 730, 829, 914, 942, 1130, 1196, 1702, 1759 |
| BspMI | ACCTGC | 1 cut(s) | 124 |
| BspOI | GCTAGC | 1 cut(s) | 80 |
| BspPI | GGATC | 4 cut(s) | 591, 756, 907, 920 |
| BspT107I | GGYRCC | 4 cut(s) | 827, 1128, 1700, 1757 |
| BspTI | CTTAAG | 2 cut(s) | 128, 1079 |
| BsrBI | CCGCTC | 5 cut(s) | 987, 1035, 1126, 1192, 1204 |
| BsrDI | GCAATG | 1 cut(s) | 1161 |
| BsrFI | RCCGGY | 2 cut(s) | 782, 824 |
| BsrI | ACTGG | 7 cut(s) | 672, 679, 692, 766, 1356, 1499, 1518 |
| BssAI | RCCGGY | 2 cut(s) | 782, 824 |
| BssMI | GATC | 5 cut(s) | 58, 231, 583, 748, 912 |
| BssNI | GRCGYC | 6 cut(s) | 361, 476, 870, 1129, 1485, 1758 |
| BssSI | CACGAG | 1 cut(s) | 416 |
| BssT1I | CCWWGG | 6 cut(s) | 149, 241, 433, 1137, 1266, 1386 |
| Bst2BI | CACGAG | 1 cut(s) | 416 |
| Bst2UI | CCWGG | 3 cut(s) | 470, 1463, 1731 |
| Bst4CI | ACNGT | 5 cut(s) | 388, 649, 737, 1279, 1561 |
| Bst6I | CTCTTC | 4 cut(s) | 63, 876, 920, 1051 |
| BstACI | GRCGYC | 6 cut(s) | 361, 476, 870, 1129, 1485, 1758 |
| BstAFI | CTTAAG | 2 cut(s) | 128, 1079 |
| BstBAI | YACGTR | 1 cut(s) | 1216 |
| BstDEI | CTNAG | 4 cut(s) | 458, 1431, 1509, 1715 |
| BstDSI | CCRYGG | 3 cut(s) | 387, 1165, 1386 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1136, 1430 |
| BstF5I | GGATG | 3 cut(s) | 1284, 1624, 1702 |
| BstFNI | CGCG | 6 cut(s) | 561, 588, 774, 899, 1167, 1409 |
| BstH2I | RGCGCY | 2 cut(s) | 1132, 1761 |
| BstHHI | GCGC | 3 cut(s) | 590, 1131, 1760 |
| BstKTI | GATC | 5 cut(s) | 61, 234, 586, 751, 915 |
| BstMAI | GTCTC | 3 cut(s) | 680, 885, 1512 |
| BstMBI | GATC | 5 cut(s) | 58, 231, 583, 748, 912 |
| BstMCI | CGRYCG | 6 cut(s) | 61, 170, 387, 1281, 1535, 1694 |
| BstNI | CCWGG | 3 cut(s) | 470, 1463, 1731 |
| BstNSI | RCATGY | 2 cut(s) | 328, 619 |
| BstSCI | CCNGG | 7 cut(s) | 468, 541, 1220, 1461, 1661, 1662, 1729 |
| BstSFI | CTRYAG | 1 cut(s) | 648 |
| BstSLI | GKGCMC | 2 cut(s) | 732, 769 |
| BstUI | CGCG | 6 cut(s) | 561, 588, 774, 899, 1167, 1409 |
| BstV1I | GCAGC | 5 cut(s) | 34, 150, 453, 766, 1369 |
| BstX2I | RGATCY | 1 cut(s) | 912 |
| BstXI | CCANNNNNNTGG | 4 cut(s) | 580, 838, 1462, 1675 |
| BstYI | RGATCY | 1 cut(s) | 912 |
| BstZI | CGGCCG | 3 cut(s) | 167, 1532, 1691 |
| Bsu15I | ATCGAT | 1 cut(s) | 194 |
| BsuI | GTATCC | 1 cut(s) | 1228 |
| BsuTUI | ATCGAT | 1 cut(s) | 194 |
| BtgI | CCRYGG | 3 cut(s) | 387, 1165, 1386 |
| BtgZI | GCGATG | 4 cut(s) | 359, 576, 755, 1694 |
| BtsCI | GGATG | 3 cut(s) | 1284, 1624, 1702 |
| BtsI | GCAGTG | 1 cut(s) | 1722 |
| BtsIMutI | CAGTG | 4 cut(s) | 665, 699, 1712, 1722 |
| BveI | ACCTGC | 1 cut(s) | 124 |
| CaiI | CAGNNNCTG | 2 cut(s) | 667, 1768 |
| CciNI | GCGGCCGC | 2 cut(s) | 167, 1532 |
| CfoI | GCGC | 3 cut(s) | 590, 1131, 1760 |
| Cfr10I | RCCGGY | 2 cut(s) | 782, 824 |
| Cfr13I | GGNCC | 9 cut(s) | 380, 637, 728, 729, 940, 1162, 1195, 1366, 1665 |
| Cfr42I | CCGCGG | 1 cut(s) | 1168 |
| Cfr9I | CCCGGG | 1 cut(s) | 1662 |
| ClaI | ATCGAT | 1 cut(s) | 194 |
| CseI | GACGC | 6 cut(s) | 53, 350, 465, 878, 905, 1474 |
| Csp6I | GTAC | 4 cut(s) | 398, 1521, 1701, 1771 |
| CviAII | CATG | 9 cut(s) | 325, 580, 616, 1006, 1370, 1387, 1607, 1675, 1753 |
| CviQI | GTAC | 4 cut(s) | 398, 1521, 1701, 1771 |
| DdeI | CTNAG | 4 cut(s) | 458, 1431, 1509, 1715 |
| DinI | GGCGCC | 2 cut(s) | 1130, 1759 |
| DpnI | GATC | 5 cut(s) | 60, 233, 585, 750, 914 |
| DpnII | GATC | 5 cut(s) | 58, 231, 583, 748, 912 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1716 |
| EaeI | YGGCCR | 5 cut(s) | 167, 960, 1263, 1532, 1691 |
| EagI | CGGCCG | 3 cut(s) | 167, 1532, 1691 |
| Eam1104I | CTCTTC | 4 cut(s) | 63, 876, 920, 1051 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1716 |
| EarI | CTCTTC | 4 cut(s) | 63, 876, 920, 1051 |
| EciI | GGCGGA | 5 cut(s) | 205, 407, 1157, 1291, 1631 |
| EclXI | CGGCCG | 3 cut(s) | 167, 1532, 1691 |
| Eco130I | CCWWGG | 6 cut(s) | 149, 241, 433, 1137, 1266, 1386 |
| Eco147I | AGGCCT | 1 cut(s) | 814 |
| Eco24I | GRGCYC | 1 cut(s) | 732 |
| Eco47I | GGWCC | 2 cut(s) | 637, 1195 |
| Eco52I | CGGCCG | 3 cut(s) | 167, 1532, 1691 |
| Eco57I | CTGAAG | 6 cut(s) | 518, 830, 1079, 1343, 1493, 1674 |
| Eco88I | CYCGRG | 3 cut(s) | 6, 725, 1662 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1136, 1430 |
| EcoO109I | RGGNCCY | 2 cut(s) | 940, 1195 |
| EcoRI | GAATTC | 1 cut(s) | 1016 |
| EcoRII | CCWGG | 3 cut(s) | 468, 1461, 1729 |
| EcoT14I | CCWWGG | 6 cut(s) | 149, 241, 433, 1137, 1266, 1386 |
| EcoT22I | ATGCAT | 1 cut(s) | 1686 |
| EcoT38I | GRGCYC | 1 cut(s) | 732 |
| EgeI | GGCGCC | 2 cut(s) | 1130, 1759 |
| EheI | GGCGCC | 2 cut(s) | 1130, 1759 |
| ErhI | CCWWGG | 6 cut(s) | 149, 241, 433, 1137, 1266, 1386 |
| Esp3I | CGTCTC | 1 cut(s) | 885 |
| FaeI | CATG | 9 cut(s) | 328, 583, 619, 1009, 1373, 1390, 1610, 1678, 1756 |
| FaqI | GGGAC | 5 cut(s) | 224, 303, 1208, 1643, 1704 |
| FatI | CATG | 9 cut(s) | 324, 579, 615, 1005, 1369, 1386, 1606, 1674, 1752 |
| FauI | CCCGC | 9 cut(s) | 91, 437, 767, 779, 951, 1028, 1172, 1185, 1540 |
| FblI | GTMKAC | 2 cut(s) | 654, 1281 |
| FokI | GGATG | 3 cut(s) | 1271, 1631, 1709 |
| FriOI | GRGCYC | 1 cut(s) | 732 |
| FspBI | CTAG | 8 cut(s) | 30, 77, 150, 992, 1199, 1572, 1640, 1746 |
| GlaI | GCGC | 3 cut(s) | 589, 1130, 1759 |
| GsaI | CCCAGC | 2 cut(s) | 630, 1765 |
| GsuI | CTGGAG | 4 cut(s) | 675, 902, 1247, 1484 |
| HaeII | RGCGCY | 2 cut(s) | 1132, 1761 |
| HapII | CCGG | 7 cut(s) | 542, 783, 825, 1222, 1568, 1663, 1694 |
| HgaI | GACGC | 6 cut(s) | 53, 350, 465, 878, 905, 1474 |
| HhaI | GCGC | 3 cut(s) | 590, 1131, 1760 |
| Hin1I | GRCGYC | 6 cut(s) | 361, 476, 870, 1129, 1485, 1758 |
| Hin1II | CATG | 9 cut(s) | 328, 583, 619, 1009, 1373, 1390, 1610, 1678, 1756 |
| Hin6I | GCGC | 3 cut(s) | 588, 1129, 1758 |
| HinP1I | GCGC | 3 cut(s) | 588, 1129, 1758 |
| HincII | GTYRAC | 2 cut(s) | 655, 1282 |
| HindII | GTYRAC | 2 cut(s) | 655, 1282 |
| HpaII | CCGG | 7 cut(s) | 542, 783, 825, 1222, 1568, 1663, 1694 |
| HphI | GGTGA | 5 cut(s) | 395, 685, 709, 964, 1444 |
| Hpy166II | GTNNAC | 5 cut(s) | 454, 655, 767, 1117, 1282 |
| Hpy188I | TCNGA | 8 cut(s) | 312, 498, 685, 849, 1027, 1059, 1654, 1738 |
| Hpy188III | TCNNGA | 6 cut(s) | 224, 490, 752, 992, 1295, 1309 |
| Hpy8I | GTNNAC | 5 cut(s) | 454, 655, 767, 1117, 1282 |
| Hpy99I | CGWCG | 6 cut(s) | 481, 737, 824, 861, 899, 1528 |
| HpyAV | CCTTC | 9 cut(s) | 172, 371, 493, 538, 1054, 1153, 1243, 1357, 1405 |
| HpyCH4III | ACNGT | 5 cut(s) | 388, 649, 737, 1279, 1561 |
| HpyCH4IV | ACGT | 4 cut(s) | 251, 1119, 1215, 1523 |
| HpyCH4V | TGCA | 8 cut(s) | 441, 767, 979, 1093, 1154, 1291, 1373, 1684 |
| HpyF3I | CTNAG | 4 cut(s) | 458, 1431, 1509, 1715 |
| HpySE526I | ACGT | 4 cut(s) | 251, 1119, 1215, 1523 |
| Hsp92I | GRCGYC | 6 cut(s) | 361, 476, 870, 1129, 1485, 1758 |
| Hsp92II | CATG | 9 cut(s) | 328, 583, 619, 1009, 1373, 1390, 1610, 1678, 1756 |
| HspAI | GCGC | 3 cut(s) | 588, 1129, 1758 |
| KasI | GGCGCC | 2 cut(s) | 1128, 1757 |
| KpnI | GGTACC | 1 cut(s) | 1704 |
| KroI | GCCGGC | 1 cut(s) | 782 |
| KroNI | GCCGGC | 1 cut(s) | 784 |
| KspI | CCGCGG | 1 cut(s) | 1168 |
| Kzo9I | GATC | 5 cut(s) | 58, 231, 583, 748, 912 |
| LmnI | GCTCC | 6 cut(s) | 789, 1228, 1252, 1318, 1570, 1732 |
| Lsp1109I | GCAGC | 5 cut(s) | 34, 150, 453, 766, 1369 |
| LweI | GCATC | 7 cut(s) | 200, 553, 690, 696, 1300, 1567, 1671 |
| MaeI | CTAG | 8 cut(s) | 30, 77, 150, 992, 1199, 1572, 1640, 1746 |
| MaeII | ACGT | 4 cut(s) | 251, 1119, 1215, 1523 |
| MaeIII | GTNAC | 5 cut(s) | 247, 545, 802, 1450, 1708 |
| MalI | GATC | 5 cut(s) | 60, 233, 585, 750, 914 |
| MbiI | CCGCTC | 5 cut(s) | 987, 1035, 1126, 1192, 1204 |
| MboI | GATC | 5 cut(s) | 58, 231, 583, 748, 912 |
| MflI | RGATCY | 1 cut(s) | 912 |
| MhlI | GDGCHC | 2 cut(s) | 732, 769 |
| MluCI | AATT | 4 cut(s) | 107, 276, 593, 1016 |
| Mly113I | GGCGCC | 2 cut(s) | 1129, 1758 |
| MlyI | GAGTC | 5 cut(s) | 206, 682, 1307, 1627, 1726 |
| MmeI | TCCRAC | 3 cut(s) | 664, 1050, 1311 |
| Mph1103I | ATGCAT | 1 cut(s) | 1686 |
| MroNI | GCCGGC | 1 cut(s) | 782 |
| MseI | TTAA | 2 cut(s) | 129, 1080 |
| MslI | CAYNNNNRTG | 2 cut(s) | 836, 1679 |
| MspA1I | CMGCKG | 7 cut(s) | 444, 626, 1167, 1382, 1547, 1630, 1768 |
| MspCI | CTTAAG | 2 cut(s) | 128, 1079 |
| MspI | CCGG | 7 cut(s) | 542, 783, 825, 1222, 1568, 1663, 1694 |
| MspR9I | CCNGG | 7 cut(s) | 470, 543, 1222, 1463, 1663, 1664, 1731 |
| MvaI | CCWGG | 3 cut(s) | 470, 1463, 1731 |
| MvnI | CGCG | 6 cut(s) | 561, 588, 774, 899, 1167, 1409 |
| NaeI | GCCGGC | 1 cut(s) | 784 |
| NarI | GGCGCC | 2 cut(s) | 1129, 1758 |
| NciI | CCSGG | 4 cut(s) | 543, 1222, 1663, 1664 |
| NcoI | CCATGG | 1 cut(s) | 1386 |
| NdeII | GATC | 5 cut(s) | 58, 231, 583, 748, 912 |
| NgoMIV | GCCGGC | 1 cut(s) | 782 |
| NheI | GCTAGC | 1 cut(s) | 76 |
| NlaIII | CATG | 9 cut(s) | 328, 583, 619, 1009, 1373, 1390, 1610, 1678, 1756 |
| NlaIV | GGNNCC | 8 cut(s) | 730, 829, 914, 942, 1130, 1196, 1702, 1759 |
| NmeAIII | GCCGAG | 1 cut(s) | 1669 |
| NmuCI | GTSAC | 4 cut(s) | 247, 802, 1450, 1708 |
| NotI | GCGGCCGC | 2 cut(s) | 167, 1532 |
| NsiI | ATGCAT | 1 cut(s) | 1686 |
| NspI | RCATGY | 2 cut(s) | 328, 619 |
| OliI | CACNNNNGTG | 1 cut(s) | 836 |
| PaeR7I | CTCGAG | 1 cut(s) | 6 |
| PaqCI | CACCTGC | 1 cut(s) | 124 |
| PceI | AGGCCT | 1 cut(s) | 814 |
| PciI | ACATGT | 1 cut(s) | 324 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 13 |
| PdiI | GCCGGC | 1 cut(s) | 784 |
| PfeI | GAWTC | 6 cut(s) | 121, 196, 493, 503, 995, 1391 |
| PflFI | GACNNNGTC | 1 cut(s) | 1496 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 327, 667 |
| Ple19I | CGATCG | 1 cut(s) | 61 |
| PleI | GAGTC | 5 cut(s) | 206, 682, 1306, 1627, 1725 |
| PluTI | GGCGCC | 2 cut(s) | 1132, 1761 |
| PpsI | GAGTC | 5 cut(s) | 206, 682, 1306, 1627, 1725 |
| Ppu21I | YACGTR | 1 cut(s) | 1216 |
| PpuMI | RGGWCCY | 1 cut(s) | 1195 |
| PscI | ACATGT | 1 cut(s) | 324 |
| Psp5II | RGGWCCY | 1 cut(s) | 1195 |
| Psp6I | CCWGG | 3 cut(s) | 468, 1461, 1729 |
| PspFI | CCCAGC | 2 cut(s) | 626, 1761 |
| PspGI | CCWGG | 3 cut(s) | 468, 1461, 1729 |
| PspN4I | GGNNCC | 8 cut(s) | 730, 829, 914, 942, 1130, 1196, 1702, 1759 |
| PspOMI | GGGCCC | 1 cut(s) | 728 |
| PspPI | GGNCC | 9 cut(s) | 380, 637, 728, 729, 940, 1162, 1195, 1366, 1665 |
| PspPPI | RGGWCCY | 1 cut(s) | 1195 |
| PspXI | VCTCGAGB | 1 cut(s) | 6 |
| PstNI | CAGNNNCTG | 2 cut(s) | 667, 1768 |
| PsuI | RGATCY | 1 cut(s) | 912 |
| PsyI | GACNNNGTC | 1 cut(s) | 1496 |
| PvuI | CGATCG | 1 cut(s) | 61 |
| RsaI | GTAC | 4 cut(s) | 399, 1522, 1702, 1772 |
| RsaNI | GTAC | 4 cut(s) | 398, 1521, 1701, 1771 |
| RseI | CAYNNNNRTG | 2 cut(s) | 836, 1679 |
| SacII | CCGCGG | 1 cut(s) | 1168 |
| SalI | GTCGAC | 2 cut(s) | 653, 1280 |
| SaqAI | TTAA | 2 cut(s) | 129, 1080 |
| Sau3AI | GATC | 5 cut(s) | 58, 231, 583, 748, 912 |
| Sau96I | GGNCC | 9 cut(s) | 380, 637, 728, 729, 940, 1162, 1195, 1366, 1665 |
| SchI | GAGTC | 5 cut(s) | 206, 682, 1307, 1627, 1726 |
| ScrFI | CCNGG | 7 cut(s) | 470, 543, 1222, 1463, 1663, 1664, 1731 |
| SduI | GDGCHC | 2 cut(s) | 732, 769 |
| SfaNI | GCATC | 7 cut(s) | 200, 553, 690, 696, 1300, 1567, 1671 |
| SfcI | CTRYAG | 1 cut(s) | 648 |
| SfoI | GGCGCC | 2 cut(s) | 1130, 1759 |
| Sfr274I | CTCGAG | 1 cut(s) | 6 |
| Sfr303I | CCGCGG | 1 cut(s) | 1168 |
| SgrAI | CRCCGGYG | 1 cut(s) | 824 |
| SgrBI | CCGCGG | 1 cut(s) | 1168 |
| SinI | GGWCC | 2 cut(s) | 637, 1195 |
| SlaI | CTCGAG | 1 cut(s) | 6 |
| SmaI | CCCGGG | 1 cut(s) | 1664 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 836, 1679 |
| SmlI | CTYRAG | 5 cut(s) | 6, 128, 222, 808, 1079 |
| SmoI | CTYRAG | 5 cut(s) | 6, 128, 222, 808, 1079 |
| Sse9I | AATT | 4 cut(s) | 107, 276, 593, 1016 |
| SseBI | AGGCCT | 1 cut(s) | 814 |
| SspDI | GGCGCC | 2 cut(s) | 1128, 1757 |
| SspMI | CTAG | 8 cut(s) | 30, 77, 150, 992, 1199, 1572, 1640, 1746 |
| StuI | AGGCCT | 1 cut(s) | 814 |
| StyD4I | CCNGG | 7 cut(s) | 468, 541, 1220, 1461, 1661, 1662, 1729 |
| StyI | CCWWGG | 6 cut(s) | 149, 241, 433, 1137, 1266, 1386 |
| TaaI | ACNGT | 5 cut(s) | 388, 649, 737, 1279, 1561 |
| TaiI | ACGT | 4 cut(s) | 254, 1122, 1218, 1526 |
| TaqI | TCGA | 9 cut(s) | 7, 124, 194, 294, 479, 489, 654, 1281, 1526 |
| TasI | AATT | 4 cut(s) | 107, 276, 593, 1016 |
| TfiI | GAWTC | 6 cut(s) | 121, 196, 493, 503, 995, 1391 |
| Tru1I | TTAA | 2 cut(s) | 129, 1080 |
| Tru9I | TTAA | 2 cut(s) | 129, 1080 |
| TscAI | CASTG | 4 cut(s) | 672, 699, 1719, 1729 |
| TseFI | GTSAC | 4 cut(s) | 247, 802, 1450, 1708 |
| TseI | GCWGC | 5 cut(s) | 47, 163, 441, 779, 1382 |
| Tsp45I | GTSAC | 4 cut(s) | 247, 802, 1450, 1708 |
| TspDTI | ATGAA | 5 cut(s) | 191, 362, 891, 1172, 1663 |
| TspMI | CCCGGG | 1 cut(s) | 1662 |
| TspRI | CASTG | 4 cut(s) | 672, 699, 1719, 1729 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1496 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 327, 667 |
| Vha464I | CTTAAG | 2 cut(s) | 128, 1079 |
| VneI | GTGCAC | 1 cut(s) | 765 |
| VpaK11BI | GGWCC | 2 cut(s) | 637, 1195 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1136, 1430 |
| XapI | RAATTY | 2 cut(s) | 276, 1016 |
| XbaI | TCTAGA | 1 cut(s) | 991 |
| XceI | RCATGY | 2 cut(s) | 328, 619 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1108 |
| XhoI | CTCGAG | 1 cut(s) | 6 |
| XmaI | CCCGGG | 1 cut(s) | 1662 |
| XmaJI | CCTAGG | 1 cut(s) | 149 |
| XmiI | GTMKAC | 2 cut(s) | 654, 1281 |
| XspI | CTAG | 8 cut(s) | 30, 77, 150, 992, 1199, 1572, 1640, 1746 |
| Zsp2I | ATGCAT | 1 cut(s) | 1686 |