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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 3 cut(s) | 251, 287, 380 |
| AccBSI | CCGCTC | 1 cut(s) | 58 |
| AccI | GTMKAC | 1 cut(s) | 267 |
| AccII | CGCG | 4 cut(s) | 18, 44, 323, 466 |
| AclWI | GGATC | 1 cut(s) | 553 |
| AcyI | GRCGYC | 2 cut(s) | 252, 288 |
| AfaI | GTAC | 2 cut(s) | 172, 505 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 377 |
| AflIII | ACRYGT | 3 cut(s) | 16, 270, 459 |
| AgsI | TTSAA | 2 cut(s) | 194, 202 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 519 |
| AjiI | CACGTC | 1 cut(s) | 460 |
| AjnI | CCWGG | 1 cut(s) | 491 |
| AluBI | AGCT | 3 cut(s) | 82, 205, 308 |
| AluI | AGCT | 3 cut(s) | 82, 205, 308 |
| Alw26I | GTCTC | 5 cut(s) | 25, 116, 344, 506, 572 |
| AlwI | GGATC | 1 cut(s) | 553 |
| Ama87I | CYCGRG | 1 cut(s) | 371 |
| ApeKI | GCWGC | 6 cut(s) | 46, 49, 94, 185, 393, 484 |
| AspLEI | GCGC | 6 cut(s) | 46, 254, 290, 325, 484, 489 |
| AspS9I | GGNCC | 3 cut(s) | 410, 426, 537 |
| AsuHPI | GGTGA | 1 cut(s) | 152 |
| AvaI | CYCGRG | 1 cut(s) | 371 |
| AvaII | GGWCC | 3 cut(s) | 410, 426, 537 |
| BaeGI | GKGCMC | 1 cut(s) | 385 |
| BanI | GGYRCC | 3 cut(s) | 251, 287, 380 |
| BbvI | GCAGC | 6 cut(s) | 33, 36, 106, 172, 405, 496 |
| BccI | CCATC | 3 cut(s) | 299, 450, 494 |
| BceAI | ACGGC | 1 cut(s) | 543 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 362, 396 |
| BciT130I | CCWGG | 1 cut(s) | 493 |
| BcoDI | GTCTC | 5 cut(s) | 25, 116, 344, 506, 572 |
| BfaI | CTAG | 1 cut(s) | 519 |
| BfmI | CTRYAG | 2 cut(s) | 50, 507 |
| BfoI | RGCGCY | 2 cut(s) | 255, 291 |
| Bme1390I | CCNGG | 1 cut(s) | 493 |
| Bme18I | GGWCC | 3 cut(s) | 410, 426, 537 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 519 |
| BmeT110I | CYCGRG | 1 cut(s) | 371 |
| BmgBI | CACGTC | 1 cut(s) | 460 |
| BmgT120I | GGNCC | 3 cut(s) | 410, 426, 537 |
| BmiI | GGNNCC | 5 cut(s) | 253, 289, 382, 427, 428 |
| BmrFI | CCNGG | 1 cut(s) | 493 |
| BmsI | GCATC | 2 cut(s) | 348, 577 |
| BpmI | CTGGAG | 1 cut(s) | 23 |
| BsaHI | GRCGYC | 2 cut(s) | 252, 288 |
| BsaI | GGTCTC | 1 cut(s) | 506 |
| BsaJI | CCNNGG | 2 cut(s) | 372, 492 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 377 |
| Bse1I | ACTGG | 1 cut(s) | 120 |
| BseBI | CCWGG | 1 cut(s) | 493 |
| BseDI | CCNNGG | 2 cut(s) | 372, 492 |
| BseGI | GGATG | 4 cut(s) | 124, 339, 442, 505 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 377 |
| BseMII | CTCAG | 2 cut(s) | 225, 567 |
| BseNI | ACTGG | 1 cut(s) | 120 |
| BseSI | GKGCMC | 1 cut(s) | 385 |
| BseXI | GCAGC | 6 cut(s) | 33, 36, 106, 172, 405, 496 |
| BseYI | CCCAGC | 1 cut(s) | 133 |
| BsgI | GTGCAG | 1 cut(s) | 113 |
| Bsh1236I | CGCG | 4 cut(s) | 18, 44, 323, 466 |
| Bsh1285I | CGRYCG | 1 cut(s) | 183 |
| BshNI | GGYRCC | 3 cut(s) | 251, 287, 380 |
| BsiEI | CGRYCG | 1 cut(s) | 183 |
| BsiHKCI | CYCGRG | 1 cut(s) | 371 |
| BslFI | GGGAC | 1 cut(s) | 412 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 377 |
| BsmAI | GTCTC | 5 cut(s) | 25, 116, 344, 506, 572 |
| BsmBI | CGTCTC | 2 cut(s) | 25, 116 |
| BsmFI | GGGAC | 1 cut(s) | 412 |
| Bso31I | GGTCTC | 1 cut(s) | 506 |
| BsoBI | CYCGRG | 1 cut(s) | 371 |
| Bsp1286I | GDGCHC | 1 cut(s) | 385 |
| Bsp143I | GATC | 3 cut(s) | 39, 541, 558 |
| BspCNI | CTCAG | 2 cut(s) | 226, 568 |
| BspFNI | CGCG | 4 cut(s) | 18, 44, 323, 466 |
| BspLI | GGNNCC | 5 cut(s) | 253, 289, 382, 427, 428 |
| BspMAI | CTGCAG | 1 cut(s) | 54 |
| BspPI | GGATC | 1 cut(s) | 553 |
| BspT107I | GGYRCC | 3 cut(s) | 251, 287, 380 |
| BspTNI | GGTCTC | 1 cut(s) | 506 |
| BsrBI | CCGCTC | 1 cut(s) | 58 |
| BsrI | ACTGG | 1 cut(s) | 120 |
| BssECI | CCNNGG | 2 cut(s) | 372, 492 |
| BssMI | GATC | 3 cut(s) | 39, 541, 558 |
| BssNI | GRCGYC | 2 cut(s) | 252, 288 |
| Bst2UI | CCWGG | 1 cut(s) | 493 |
| BstACI | GRCGYC | 2 cut(s) | 252, 288 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 365 |
| BstC8I | GCNNGC | 4 cut(s) | 155, 250, 468, 532 |
| BstDEI | CTNAG | 2 cut(s) | 234, 576 |
| BstF5I | GGATG | 4 cut(s) | 124, 339, 442, 505 |
| BstFNI | CGCG | 4 cut(s) | 18, 44, 323, 466 |
| BstH2I | RGCGCY | 2 cut(s) | 255, 291 |
| BstHHI | GCGC | 6 cut(s) | 46, 254, 290, 325, 484, 489 |
| BstKTI | GATC | 3 cut(s) | 42, 544, 561 |
| BstMAI | GTCTC | 5 cut(s) | 25, 116, 344, 506, 572 |
| BstMBI | GATC | 3 cut(s) | 39, 541, 558 |
| BstMCI | CGRYCG | 1 cut(s) | 183 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 55, 185, 194, 245, 322, 365 |
| BstNI | CCWGG | 1 cut(s) | 493 |
| BstNSI | RCATGY | 1 cut(s) | 274 |
| BstSCI | CCNGG | 1 cut(s) | 491 |
| BstSFI | CTRYAG | 2 cut(s) | 50, 507 |
| BstSLI | GKGCMC | 1 cut(s) | 385 |
| BstUI | CGCG | 4 cut(s) | 18, 44, 323, 466 |
| BstV1I | GCAGC | 6 cut(s) | 33, 36, 106, 172, 405, 496 |
| BtgZI | GCGATG | 1 cut(s) | 242 |
| BtrI | CACGTC | 1 cut(s) | 460 |
| BtsCI | GGATG | 4 cut(s) | 124, 339, 442, 505 |
| BtsIMutI | CAGTG | 1 cut(s) | 573 |
| Cac8I | GCNNGC | 4 cut(s) | 155, 250, 468, 532 |
| CfoI | GCGC | 6 cut(s) | 46, 254, 290, 325, 484, 489 |
| Cfr13I | GGNCC | 3 cut(s) | 410, 426, 537 |
| CseI | GACGC | 2 cut(s) | 7, 329 |
| Csp6I | GTAC | 2 cut(s) | 171, 504 |
| CviAII | CATG | 1 cut(s) | 271 |
| CviJI | RGCY | 7 cut(s) | 82, 133, 205, 248, 308, 316, 356 |
| CviKI_1 | RGCY | 7 cut(s) | 82, 133, 205, 248, 308, 316, 356 |
| CviQI | GTAC | 2 cut(s) | 171, 504 |
| DdeI | CTNAG | 2 cut(s) | 234, 576 |
| DinI | GGCGCC | 2 cut(s) | 253, 289 |
| DpnI | GATC | 3 cut(s) | 41, 543, 560 |
| DpnII | GATC | 3 cut(s) | 39, 541, 558 |
| DriI | GACNNNNNGTC | 1 cut(s) | 519 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 519 |
| EciI | GGCGGA | 4 cut(s) | 227, 295, 423, 512 |
| Eco31I | GGTCTC | 1 cut(s) | 506 |
| Eco47I | GGWCC | 3 cut(s) | 410, 426, 537 |
| Eco88I | CYCGRG | 1 cut(s) | 371 |
| EcoO109I | RGGNCCY | 1 cut(s) | 426 |
| EcoRII | CCWGG | 1 cut(s) | 491 |
| EgeI | GGCGCC | 2 cut(s) | 253, 289 |
| EheI | GGCGCC | 2 cut(s) | 253, 289 |
| Esp3I | CGTCTC | 2 cut(s) | 25, 116 |
| FaeI | CATG | 1 cut(s) | 274 |
| FaiI | YATR | 4 cut(s) | 219, 272, 416, 509 |
| FaqI | GGGAC | 1 cut(s) | 412 |
| FatI | CATG | 1 cut(s) | 270 |
| FauI | CCCGC | 1 cut(s) | 90 |
| FblI | GTMKAC | 1 cut(s) | 267 |
| FokI | GGATG | 4 cut(s) | 131, 326, 429, 512 |
| FspBI | CTAG | 1 cut(s) | 519 |
| GlaI | GCGC | 6 cut(s) | 45, 253, 289, 324, 483, 488 |
| GsaI | CCCAGC | 1 cut(s) | 137 |
| GsuI | CTGGAG | 1 cut(s) | 23 |
| HaeII | RGCGCY | 2 cut(s) | 255, 291 |
| HgaI | GACGC | 2 cut(s) | 7, 329 |
| HhaI | GCGC | 6 cut(s) | 46, 254, 290, 325, 484, 489 |
| Hin1I | GRCGYC | 2 cut(s) | 252, 288 |
| Hin1II | CATG | 1 cut(s) | 274 |
| Hin6I | GCGC | 6 cut(s) | 44, 252, 288, 323, 482, 487 |
| HinP1I | GCGC | 6 cut(s) | 44, 252, 288, 323, 482, 487 |
| HindIII | AAGCTT | 1 cut(s) | 306 |
| HinfI | GANTC | 2 cut(s) | 101, 550 |
| HphI | GGTGA | 1 cut(s) | 152 |
| Hpy166II | GTNNAC | 1 cut(s) | 268 |
| Hpy188I | TCNGA | 3 cut(s) | 457, 525, 541 |
| Hpy188III | TCNNGA | 1 cut(s) | 371 |
| Hpy8I | GTNNAC | 1 cut(s) | 268 |
| Hpy99I | CGWCG | 2 cut(s) | 457, 529 |
| HpyAV | CCTTC | 4 cut(s) | 238, 277, 416, 587 |
| HpyCH4IV | ACGT | 2 cut(s) | 264, 459 |
| HpyCH4V | TGCA | 3 cut(s) | 52, 94, 157 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 55, 185, 194, 245, 322, 365 |
| HpyF3I | CTNAG | 2 cut(s) | 234, 576 |
| HpySE526I | ACGT | 2 cut(s) | 264, 459 |
| Hsp92I | GRCGYC | 2 cut(s) | 252, 288 |
| Hsp92II | CATG | 1 cut(s) | 274 |
| HspAI | GCGC | 6 cut(s) | 44, 252, 288, 323, 482, 487 |
| KasI | GGCGCC | 2 cut(s) | 251, 287 |
| KflI | GGGWCCC | 1 cut(s) | 426 |
| Kzo9I | GATC | 3 cut(s) | 39, 541, 558 |
| LmnI | GCTCC | 3 cut(s) | 31, 55, 373 |
| Lsp1109I | GCAGC | 6 cut(s) | 33, 36, 106, 172, 405, 496 |
| LweI | GCATC | 2 cut(s) | 348, 577 |
| MaeI | CTAG | 1 cut(s) | 519 |
| MaeII | ACGT | 2 cut(s) | 264, 459 |
| MaeIII | GTNAC | 1 cut(s) | 551 |
| MalI | GATC | 3 cut(s) | 41, 543, 560 |
| MbiI | CCGCTC | 1 cut(s) | 58 |
| MboI | GATC | 3 cut(s) | 39, 541, 558 |
| MhlI | GDGCHC | 1 cut(s) | 385 |
| MluI | ACGCGT | 1 cut(s) | 16 |
| Mly113I | GGCGCC | 2 cut(s) | 252, 288 |
| MlyI | GAGTC | 2 cut(s) | 110, 559 |
| MmeI | TCCRAC | 3 cut(s) | 9, 435, 548 |
| MnlI | CCTC | 5 cut(s) | 184, 229, 270, 367, 371 |
| MslI | CAYNNNNRTG | 1 cut(s) | 89 |
| MspA1I | CMGCKG | 2 cut(s) | 97, 396 |
| MspR9I | CCNGG | 1 cut(s) | 493 |
| MteI | GCGCNGCGC | 1 cut(s) | 485 |
| MvaI | CCWGG | 1 cut(s) | 493 |
| MvnI | CGCG | 4 cut(s) | 18, 44, 323, 466 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 55, 185, 194, 245, 322, 365 |
| NarI | GGCGCC | 2 cut(s) | 252, 288 |
| NdeII | GATC | 3 cut(s) | 39, 541, 558 |
| NlaIII | CATG | 1 cut(s) | 274 |
| NlaIV | GGNNCC | 5 cut(s) | 253, 289, 382, 427, 428 |
| NmuCI | GTSAC | 1 cut(s) | 551 |
| NspI | RCATGY | 1 cut(s) | 274 |
| PciI | ACATGT | 1 cut(s) | 270 |
| PleI | GAGTC | 2 cut(s) | 109, 558 |
| PluTI | GGCGCC | 2 cut(s) | 255, 291 |
| PpsI | GAGTC | 2 cut(s) | 109, 558 |
| PpuMI | RGGWCCY | 1 cut(s) | 426 |
| PscI | ACATGT | 1 cut(s) | 270 |
| Psp5II | RGGWCCY | 1 cut(s) | 426 |
| Psp6I | CCWGG | 1 cut(s) | 491 |
| PspFI | CCCAGC | 1 cut(s) | 133 |
| PspGI | CCWGG | 1 cut(s) | 491 |
| PspN4I | GGNNCC | 5 cut(s) | 253, 289, 382, 427, 428 |
| PspPI | GGNCC | 3 cut(s) | 410, 426, 537 |
| PspPPI | RGGWCCY | 1 cut(s) | 426 |
| PstI | CTGCAG | 1 cut(s) | 54 |
| RsaI | GTAC | 2 cut(s) | 172, 505 |
| RsaNI | GTAC | 2 cut(s) | 171, 504 |
| RseI | CAYNNNNRTG | 1 cut(s) | 89 |
| Sau3AI | GATC | 3 cut(s) | 39, 541, 558 |
| Sau96I | GGNCC | 3 cut(s) | 410, 426, 537 |
| SchI | GAGTC | 2 cut(s) | 110, 559 |
| ScrFI | CCNGG | 1 cut(s) | 493 |
| SduI | GDGCHC | 1 cut(s) | 385 |
| SetI | ASST | 9 cut(s) | 84, 176, 207, 267, 310, 382, 415, 462, 494 |
| SfaNI | GCATC | 2 cut(s) | 348, 577 |
| SfcI | CTRYAG | 2 cut(s) | 50, 507 |
| SfoI | GGCGCC | 2 cut(s) | 253, 289 |
| SinI | GGWCC | 3 cut(s) | 410, 426, 537 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 89 |
| SspDI | GGCGCC | 2 cut(s) | 251, 287 |
| SspMI | CTAG | 1 cut(s) | 519 |
| StyD4I | CCNGG | 1 cut(s) | 491 |
| TaiI | ACGT | 2 cut(s) | 267, 462 |
| TaqI | TCGA | 2 cut(s) | 294, 557 |
| TauI | GCSGC | 7 cut(s) | 68, 110, 133, 242, 359, 568, 571 |
| TscAI | CASTG | 1 cut(s) | 580 |
| TseFI | GTSAC | 1 cut(s) | 551 |
| TseI | GCWGC | 6 cut(s) | 46, 49, 94, 185, 393, 484 |
| Tsp45I | GTSAC | 1 cut(s) | 551 |
| TspDTI | ATGAA | 1 cut(s) | 27 |
| TspRI | CASTG | 1 cut(s) | 580 |
| VpaK11BI | GGWCC | 3 cut(s) | 410, 426, 537 |
| XceI | RCATGY | 1 cut(s) | 274 |
| XmiI | GTMKAC | 1 cut(s) | 267 |
| XspI | CTAG | 1 cut(s) | 519 |