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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 33, 963 |
| Acc65I | GGTACC | 1 cut(s) | 347 |
| AccB1I | GGYRCC | 5 cut(s) | 347, 647, 935, 971, 1187 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 310, 981 |
| AccBSI | CCGCTC | 3 cut(s) | 147, 679, 1214 |
| AccII | CGCG | 4 cut(s) | 120, 654, 858, 1069 |
| AclWI | GGATC | 3 cut(s) | 209, 766, 1233 |
| AcoI | YGGCCR | 1 cut(s) | 411 |
| AcsI | RAATTY | 1 cut(s) | 160 |
| AcuI | CTGAAG | 2 cut(s) | 761, 1161 |
| AcvI | CACGTG | 1 cut(s) | 83 |
| AcyI | GRCGYC | 2 cut(s) | 972, 1199 |
| AfaI | GTAC | 3 cut(s) | 349, 509, 1009 |
| AflII | CTTAAG | 1 cut(s) | 71 |
| AflIII | ACRYGT | 1 cut(s) | 80 |
| AgeI | ACCGGT | 1 cut(s) | 1258 |
| AgsI | TTSAA | 3 cut(s) | 368, 580, 736 |
| AjnI | CCWGG | 4 cut(s) | 165, 225, 303, 1239 |
| AleI | CACNNNNGTG | 1 cut(s) | 1193 |
| Alw21I | GWGCWC | 1 cut(s) | 1208 |
| Alw26I | GTCTC | 5 cut(s) | 263, 416, 484, 569, 599 |
| AlwI | GGATC | 3 cut(s) | 209, 766, 1233 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 913 |
| AoxI | GGCC | 4 cut(s) | 135, 411, 585, 909 |
| ApeKI | GCWGC | 6 cut(s) | 182, 199, 466, 1021, 1166, 1280 |
| ApoI | RAATTY | 1 cut(s) | 160 |
| AsiGI | ACCGGT | 1 cut(s) | 1258 |
| Asp718I | GGTACC | 1 cut(s) | 347 |
| AspLEI | GCGC | 5 cut(s) | 199, 232, 860, 974, 1069 |
| AspS9I | GGNCC | 6 cut(s) | 142, 586, 739, 910, 952, 1097 |
| AsuC2I | CCSGG | 2 cut(s) | 102, 835 |
| AsuHPI | GGTGA | 5 cut(s) | 87, 484, 1094, 1207, 1229 |
| AsuNHI | GCTAGC | 1 cut(s) | 745 |
| AvaII | GGWCC | 4 cut(s) | 142, 739, 952, 1097 |
| BaeGI | GKGCMC | 1 cut(s) | 652 |
| BanI | GGYRCC | 5 cut(s) | 347, 647, 935, 971, 1187 |
| BanII | GRGCYC | 1 cut(s) | 1208 |
| BauI | CACGAG | 1 cut(s) | 1215 |
| BbrPI | CACGTG | 1 cut(s) | 83 |
| BbsI | GAAGAC | 1 cut(s) | 885 |
| Bbv12I | GWGCWC | 1 cut(s) | 1208 |
| BbvI | GCAGC | 6 cut(s) | 194, 211, 478, 1033, 1178, 1267 |
| BccI | CCATC | 3 cut(s) | 434, 910, 1178 |
| BceAI | ACGGC | 2 cut(s) | 1031, 1268 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 10, 44 |
| BciT130I | CCWGG | 4 cut(s) | 167, 227, 305, 1241 |
| BciVI | GTATCC | 1 cut(s) | 715 |
| BclI | TGATCA | 1 cut(s) | 1090 |
| BcnI | CCSGG | 2 cut(s) | 102, 835 |
| BcoDI | GTCTC | 5 cut(s) | 263, 416, 484, 569, 599 |
| BfaI | CTAG | 3 cut(s) | 545, 746, 1176 |
| BfoI | RGCGCY | 1 cut(s) | 975 |
| BfrI | CTTAAG | 1 cut(s) | 71 |
| BfuAI | ACCTGC | 2 cut(s) | 33, 963 |
| BfuI | GTATCC | 1 cut(s) | 715 |
| BlpI | GCTNAGC | 2 cut(s) | 279, 453 |
| Bme1390I | CCNGG | 6 cut(s) | 102, 167, 227, 305, 835, 1241 |
| Bme18I | GGWCC | 4 cut(s) | 142, 739, 952, 1097 |
| BmgT120I | GGNCC | 6 cut(s) | 142, 586, 739, 910, 952, 1097 |
| BmrFI | CCNGG | 6 cut(s) | 102, 167, 227, 305, 835, 1241 |
| BmrI | ACTGGG | 1 cut(s) | 703 |
| BmsI | GCATC | 4 cut(s) | 241, 379, 628, 1001 |
| BmtI | GCTAGC | 1 cut(s) | 749 |
| BmuI | ACTGGG | 1 cut(s) | 703 |
| BoxI | GACNNNNGTC | 1 cut(s) | 209 |
| BpiI | GAAGAC | 1 cut(s) | 885 |
| BpmI | CTGGAG | 3 cut(s) | 188, 551, 1080 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 279, 453 |
| BpuMI | CCSGG | 2 cut(s) | 102, 835 |
| BsaAI | YACGTR | 1 cut(s) | 83 |
| BsaHI | GRCGYC | 2 cut(s) | 972, 1199 |
| BsaI | GGTCTC | 3 cut(s) | 416, 569, 599 |
| BsaJI | CCNNGG | 7 cut(s) | 138, 225, 255, 304, 624, 652, 1191 |
| BsaWI | WCCGGW | 2 cut(s) | 245, 1258 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 161, 191, 1260, 1290 |
| Bse118I | RCCGGY | 3 cut(s) | 469, 938, 1258 |
| Bse1I | ACTGG | 5 cut(s) | 114, 709, 1005, 1031, 1063 |
| Bse3DI | GCAATG | 1 cut(s) | 723 |
| BseBI | CCWGG | 4 cut(s) | 167, 227, 305, 1241 |
| BseDI | CCNNGG | 7 cut(s) | 138, 225, 255, 304, 624, 652, 1191 |
| BseGI | GGATG | 3 cut(s) | 829, 1111, 1189 |
| BseMI | GCAATG | 1 cut(s) | 723 |
| BseMII | CTCAG | 2 cut(s) | 270, 1067 |
| BseNI | ACTGG | 5 cut(s) | 114, 709, 1005, 1031, 1063 |
| BseRI | GAGGAG | 3 cut(s) | 563, 566, 865 |
| BseSI | GKGCMC | 1 cut(s) | 652 |
| BseX3I | CGGCCG | 1 cut(s) | 411 |
| BseXI | GCAGC | 6 cut(s) | 194, 211, 478, 1033, 1178, 1267 |
| BseYI | CCCAGC | 2 cut(s) | 185, 838 |
| BsgI | GTGCAG | 1 cut(s) | 281 |
| Bsh1236I | CGCG | 4 cut(s) | 120, 654, 858, 1069 |
| Bsh1285I | CGRYCG | 1 cut(s) | 414 |
| BshFI | GGCC | 4 cut(s) | 137, 413, 587, 911 |
| BshNI | GGYRCC | 5 cut(s) | 347, 647, 935, 971, 1187 |
| BshTI | ACCGGT | 1 cut(s) | 1258 |
| BsiEI | CGRYCG | 1 cut(s) | 414 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1208 |
| BsiSI | CCGG | 7 cut(s) | 101, 246, 470, 835, 939, 1100, 1259 |
| BslFI | GGGAC | 4 cut(s) | 323, 695, 1054, 1132 |
| BsmAI | GTCTC | 5 cut(s) | 263, 416, 484, 569, 599 |
| BsmFI | GGGAC | 4 cut(s) | 323, 695, 1054, 1132 |
| BsnI | GGCC | 4 cut(s) | 137, 413, 587, 911 |
| Bso31I | GGTCTC | 3 cut(s) | 416, 569, 599 |
| Bsp1286I | GDGCHC | 2 cut(s) | 652, 1208 |
| Bsp143I | GATC | 7 cut(s) | 31, 214, 502, 555, 771, 1090, 1225 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 279, 453 |
| Bsp19I | CCATGG | 2 cut(s) | 138, 1191 |
| Bsp68I | TCGCGA | 1 cut(s) | 120 |
| BspANI | GGCC | 4 cut(s) | 137, 413, 587, 911 |
| BspCNI | CTCAG | 2 cut(s) | 271, 1066 |
| BspFNI | CGCG | 4 cut(s) | 120, 654, 858, 1069 |
| BspMI | ACCTGC | 2 cut(s) | 33, 963 |
| BspOI | GCTAGC | 1 cut(s) | 749 |
| BspPI | GGATC | 3 cut(s) | 209, 766, 1233 |
| BspT107I | GGYRCC | 5 cut(s) | 347, 647, 935, 971, 1187 |
| BspTI | CTTAAG | 1 cut(s) | 71 |
| BspTNI | GGTCTC | 3 cut(s) | 416, 569, 599 |
| BsrBI | CCGCTC | 3 cut(s) | 147, 679, 1214 |
| BsrDI | GCAATG | 1 cut(s) | 723 |
| BsrFI | RCCGGY | 3 cut(s) | 469, 938, 1258 |
| BsrI | ACTGG | 5 cut(s) | 114, 709, 1005, 1031, 1063 |
| BssAI | RCCGGY | 3 cut(s) | 469, 938, 1258 |
| BssECI | CCNNGG | 7 cut(s) | 138, 225, 255, 304, 624, 652, 1191 |
| BssMI | GATC | 7 cut(s) | 31, 214, 502, 555, 771, 1090, 1225 |
| BssNI | GRCGYC | 2 cut(s) | 972, 1199 |
| BssSI | CACGAG | 1 cut(s) | 1215 |
| BssT1I | CCWWGG | 3 cut(s) | 138, 624, 1191 |
| Bst2BI | CACGAG | 1 cut(s) | 1215 |
| Bst2UI | CCWGG | 4 cut(s) | 167, 227, 305, 1241 |
| Bst4CI | ACNGT | 3 cut(s) | 211, 256, 478 |
| BstACI | GRCGYC | 2 cut(s) | 972, 1199 |
| BstAFI | CTTAAG | 1 cut(s) | 71 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1027 |
| BstBAI | YACGTR | 1 cut(s) | 83 |
| BstDEI | CTNAG | 5 cut(s) | 279, 453, 662, 759, 1053 |
| BstDSI | CCRYGG | 4 cut(s) | 138, 255, 652, 1191 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 917 |
| BstF5I | GGATG | 3 cut(s) | 829, 1111, 1189 |
| BstFNI | CGCG | 4 cut(s) | 120, 654, 858, 1069 |
| BstH2I | RGCGCY | 1 cut(s) | 975 |
| BstHHI | GCGC | 5 cut(s) | 199, 232, 860, 974, 1069 |
| BstKTI | GATC | 7 cut(s) | 34, 217, 505, 558, 774, 1093, 1228 |
| BstMAI | GTCTC | 5 cut(s) | 263, 416, 484, 569, 599 |
| BstMBI | GATC | 7 cut(s) | 31, 214, 502, 555, 771, 1090, 1225 |
| BstMCI | CGRYCG | 1 cut(s) | 414 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 191, 196, 416, 458, 463, 647, 755, 1027 |
| BstNI | CCWGG | 4 cut(s) | 167, 227, 305, 1241 |
| BstNSI | RCATGY | 2 cut(s) | 178, 323 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 209 |
| BstSCI | CCNGG | 6 cut(s) | 100, 165, 225, 303, 833, 1239 |
| BstSLI | GKGCMC | 1 cut(s) | 652 |
| BstUI | CGCG | 4 cut(s) | 120, 654, 858, 1069 |
| BstV1I | GCAGC | 6 cut(s) | 194, 211, 478, 1033, 1178, 1267 |
| BstV2I | GAAGAC | 1 cut(s) | 885 |
| BstX2I | RGATCY | 1 cut(s) | 771 |
| BstYI | RGATCY | 1 cut(s) | 771 |
| BstZI | CGGCCG | 1 cut(s) | 411 |
| BsuI | GTATCC | 1 cut(s) | 715 |
| BsuRI | GGCC | 4 cut(s) | 137, 413, 587, 911 |
| BtgI | CCRYGG | 4 cut(s) | 138, 255, 652, 1191 |
| BtgZI | GCGATG | 1 cut(s) | 651 |
| BtsCI | GGATG | 3 cut(s) | 829, 1111, 1189 |
| BtsI | GCAGTG | 1 cut(s) | 1084 |
| BtsIMutI | CAGTG | 3 cut(s) | 207, 1038, 1084 |
| BtuMI | TCGCGA | 1 cut(s) | 120 |
| BveI | ACCTGC | 2 cut(s) | 33, 963 |
| CaiI | CAGNNNCTG | 1 cut(s) | 913 |
| CfoI | GCGC | 5 cut(s) | 199, 232, 860, 974, 1069 |
| Cfr10I | RCCGGY | 3 cut(s) | 469, 938, 1258 |
| Cfr13I | GGNCC | 6 cut(s) | 142, 586, 739, 910, 952, 1097 |
| Cfr42I | CCGCGG | 1 cut(s) | 655 |
| CseI | GACGC | 1 cut(s) | 1207 |
| CsiI | ACCWGGT | 1 cut(s) | 1239 |
| Csp6I | GTAC | 3 cut(s) | 348, 508, 1008 |
| CspAI | ACCGGT | 1 cut(s) | 1258 |
| CviQI | GTAC | 3 cut(s) | 348, 508, 1008 |
| DdeI | CTNAG | 5 cut(s) | 279, 453, 662, 759, 1053 |
| DinI | GGCGCC | 1 cut(s) | 973 |
| DpnI | GATC | 7 cut(s) | 33, 216, 504, 557, 773, 1092, 1227 |
| DpnII | GATC | 7 cut(s) | 31, 214, 502, 555, 771, 1090, 1225 |
| EaeI | YGGCCR | 1 cut(s) | 411 |
| EagI | CGGCCG | 1 cut(s) | 411 |
| EciI | GGCGGA | 4 cut(s) | 28, 617, 1118, 1281 |
| Ecl136II | GAGCTC | 1 cut(s) | 1206 |
| EclXI | CGGCCG | 1 cut(s) | 411 |
| Eco130I | CCWWGG | 3 cut(s) | 138, 624, 1191 |
| Eco24I | GRGCYC | 1 cut(s) | 1208 |
| Eco31I | GGTCTC | 3 cut(s) | 416, 569, 599 |
| Eco47I | GGWCC | 4 cut(s) | 142, 739, 952, 1097 |
| Eco52I | CGGCCG | 1 cut(s) | 411 |
| Eco53kI | GAGCTC | 1 cut(s) | 1206 |
| Eco57I | CTGAAG | 2 cut(s) | 761, 1161 |
| Eco72I | CACGTG | 1 cut(s) | 83 |
| EcoICRI | GAGCTC | 1 cut(s) | 1206 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 917 |
| EcoO109I | RGGNCCY | 1 cut(s) | 910 |
| EcoRII | CCWGG | 4 cut(s) | 165, 225, 303, 1239 |
| EcoT14I | CCWWGG | 3 cut(s) | 138, 624, 1191 |
| EcoT38I | GRGCYC | 1 cut(s) | 1208 |
| EgeI | GGCGCC | 1 cut(s) | 973 |
| EheI | GGCGCC | 1 cut(s) | 973 |
| ErhI | CCWWGG | 3 cut(s) | 138, 624, 1191 |
| FaqI | GGGAC | 4 cut(s) | 323, 695, 1054, 1132 |
| FauI | CCCGC | 4 cut(s) | 349, 659, 672, 1031 |
| FbaI | TGATCA | 1 cut(s) | 1090 |
| FokI | GGATG | 3 cut(s) | 836, 1118, 1196 |
| FriOI | GRGCYC | 1 cut(s) | 1208 |
| FspBI | CTAG | 3 cut(s) | 545, 746, 1176 |
| GlaI | GCGC | 5 cut(s) | 198, 231, 859, 973, 1068 |
| GsaI | CCCAGC | 2 cut(s) | 189, 842 |
| GsuI | CTGGAG | 3 cut(s) | 188, 551, 1080 |
| HaeII | RGCGCY | 1 cut(s) | 975 |
| HaeIII | GGCC | 4 cut(s) | 137, 413, 587, 911 |
| HapII | CCGG | 7 cut(s) | 101, 246, 470, 835, 939, 1100, 1259 |
| HgaI | GACGC | 1 cut(s) | 1207 |
| HhaI | GCGC | 5 cut(s) | 199, 232, 860, 974, 1069 |
| Hin1I | GRCGYC | 2 cut(s) | 972, 1199 |
| Hin6I | GCGC | 5 cut(s) | 197, 230, 858, 972, 1067 |
| HinP1I | GCGC | 5 cut(s) | 197, 230, 858, 972, 1067 |
| HincII | GTYRAC | 2 cut(s) | 67, 78 |
| HindII | GTYRAC | 2 cut(s) | 67, 78 |
| HindIII | AAGCTT | 2 cut(s) | 459, 659 |
| HinfI | GANTC | 7 cut(s) | 92, 271, 785, 878, 1109, 1120, 1294 |
| HpaII | CCGG | 7 cut(s) | 101, 246, 470, 835, 939, 1100, 1259 |
| HphI | GGTGA | 5 cut(s) | 87, 484, 1094, 1207, 1229 |
| Hpy166II | GTNNAC | 2 cut(s) | 67, 78 |
| Hpy188I | TCNGA | 8 cut(s) | 252, 431, 537, 1056, 1141, 1225, 1230, 1290 |
| Hpy188III | TCNNGA | 3 cut(s) | 119, 545, 568 |
| Hpy8I | GTNNAC | 2 cut(s) | 67, 78 |
| HpyAV | CCTTC | 7 cut(s) | 442, 574, 730, 748, 785, 922, 961 |
| HpyCH4III | ACNGT | 3 cut(s) | 211, 256, 478 |
| HpyCH4IV | ACGT | 2 cut(s) | 82, 1010 |
| HpyCH4V | TGCA | 3 cut(s) | 21, 182, 262 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 191, 196, 416, 458, 463, 647, 755, 1027 |
| HpyF3I | CTNAG | 5 cut(s) | 279, 453, 662, 759, 1053 |
| HpySE526I | ACGT | 2 cut(s) | 82, 1010 |
| Hsp92I | GRCGYC | 2 cut(s) | 972, 1199 |
| HspAI | GCGC | 5 cut(s) | 197, 230, 858, 972, 1067 |
| KasI | GGCGCC | 1 cut(s) | 971 |
| KpnI | GGTACC | 1 cut(s) | 351 |
| Ksp22I | TGATCA | 1 cut(s) | 1090 |
| KspI | CCGCGG | 1 cut(s) | 655 |
| Kzo9I | GATC | 7 cut(s) | 31, 214, 502, 555, 771, 1090, 1225 |
| LmnI | GCTCC | 7 cut(s) | 152, 239, 300, 313, 431, 715, 852 |
| Lsp1109I | GCAGC | 6 cut(s) | 194, 211, 478, 1033, 1178, 1267 |
| LweI | GCATC | 4 cut(s) | 241, 379, 628, 1001 |
| MabI | ACCWGGT | 1 cut(s) | 1239 |
| MaeI | CTAG | 3 cut(s) | 545, 746, 1176 |
| MaeII | ACGT | 2 cut(s) | 82, 1010 |
| MaeIII | GTNAC | 4 cut(s) | 104, 472, 1195, 1235 |
| MalI | GATC | 7 cut(s) | 33, 216, 504, 557, 773, 1092, 1227 |
| MbiI | CCGCTC | 3 cut(s) | 147, 679, 1214 |
| MboI | GATC | 7 cut(s) | 31, 214, 502, 555, 771, 1090, 1225 |
| MboII | GAAGA | 4 cut(s) | 356, 413, 457, 885 |
| MflI | RGATCY | 1 cut(s) | 771 |
| MhlI | GDGCHC | 2 cut(s) | 652, 1208 |
| MluCI | AATT | 1 cut(s) | 160 |
| Mly113I | GGCGCC | 1 cut(s) | 972 |
| MlyI | GAGTC | 2 cut(s) | 265, 1114 |
| MmeI | TCCRAC | 2 cut(s) | 291, 693 |
| MseI | TTAA | 3 cut(s) | 72, 885, 1245 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1193 |
| MspA1I | CMGCKG | 5 cut(s) | 185, 344, 654, 781, 1117 |
| MspCI | CTTAAG | 1 cut(s) | 71 |
| MspI | CCGG | 7 cut(s) | 101, 246, 470, 835, 939, 1100, 1259 |
| MspR9I | CCNGG | 6 cut(s) | 102, 167, 227, 305, 835, 1241 |
| MvaI | CCWGG | 4 cut(s) | 167, 227, 305, 1241 |
| MvnI | CGCG | 4 cut(s) | 120, 654, 858, 1069 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 191, 196, 416, 458, 463, 647, 755, 1027 |
| NarI | GGCGCC | 1 cut(s) | 972 |
| NciI | CCSGG | 2 cut(s) | 102, 835 |
| NcoI | CCATGG | 2 cut(s) | 138, 1191 |
| NdeII | GATC | 7 cut(s) | 31, 214, 502, 555, 771, 1090, 1225 |
| NheI | GCTAGC | 1 cut(s) | 745 |
| NmeAIII | GCCGAG | 1 cut(s) | 1227 |
| NmuCI | GTSAC | 3 cut(s) | 472, 1195, 1235 |
| NruI | TCGCGA | 1 cut(s) | 120 |
| NspI | RCATGY | 2 cut(s) | 178, 323 |
| OliI | CACNNNNGTG | 1 cut(s) | 1193 |
| PaeI | GCATGC | 1 cut(s) | 323 |
| PfeI | GAWTC | 5 cut(s) | 92, 785, 878, 1109, 1294 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 310, 981 |
| PfoI | TCCNGGA | 1 cut(s) | 165 |
| PinAI | ACCGGT | 1 cut(s) | 1258 |
| PleI | GAGTC | 2 cut(s) | 265, 1114 |
| PluTI | GGCGCC | 1 cut(s) | 975 |
| PmaCI | CACGTG | 1 cut(s) | 83 |
| PmlI | CACGTG | 1 cut(s) | 83 |
| PpsI | GAGTC | 2 cut(s) | 265, 1114 |
| Ppu21I | YACGTR | 1 cut(s) | 83 |
| PshAI | GACNNNNGTC | 1 cut(s) | 209 |
| Psp124BI | GAGCTC | 1 cut(s) | 1208 |
| Psp6I | CCWGG | 4 cut(s) | 165, 225, 303, 1239 |
| PspCI | CACGTG | 1 cut(s) | 83 |
| PspFI | CCCAGC | 2 cut(s) | 185, 838 |
| PspGI | CCWGG | 4 cut(s) | 165, 225, 303, 1239 |
| PspPI | GGNCC | 6 cut(s) | 142, 586, 739, 910, 952, 1097 |
| PstNI | CAGNNNCTG | 1 cut(s) | 913 |
| PsuI | RGATCY | 1 cut(s) | 771 |
| PvuII | CAGCTG | 3 cut(s) | 185, 781, 1117 |
| RruI | TCGCGA | 1 cut(s) | 120 |
| RsaI | GTAC | 3 cut(s) | 349, 509, 1009 |
| RsaNI | GTAC | 3 cut(s) | 348, 508, 1008 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1193 |
| SacI | GAGCTC | 1 cut(s) | 1208 |
| SacII | CCGCGG | 1 cut(s) | 655 |
| SaqAI | TTAA | 3 cut(s) | 72, 885, 1245 |
| Sau3AI | GATC | 7 cut(s) | 31, 214, 502, 555, 771, 1090, 1225 |
| Sau96I | GGNCC | 6 cut(s) | 142, 586, 739, 910, 952, 1097 |
| SchI | GAGTC | 2 cut(s) | 265, 1114 |
| ScrFI | CCNGG | 6 cut(s) | 102, 167, 227, 305, 835, 1241 |
| SduI | GDGCHC | 2 cut(s) | 652, 1208 |
| SexAI | ACCWGGT | 1 cut(s) | 1239 |
| SfaNI | GCATC | 4 cut(s) | 241, 379, 628, 1001 |
| SfoI | GGCGCC | 1 cut(s) | 973 |
| Sfr303I | CCGCGG | 1 cut(s) | 655 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1258 |
| SgrBI | CCGCGG | 1 cut(s) | 655 |
| SinI | GGWCC | 4 cut(s) | 142, 739, 952, 1097 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1193 |
| SmlI | CTYRAG | 1 cut(s) | 71 |
| SmoI | CTYRAG | 1 cut(s) | 71 |
| SphI | GCATGC | 1 cut(s) | 323 |
| Sse9I | AATT | 1 cut(s) | 160 |
| SspDI | GGCGCC | 1 cut(s) | 971 |
| SspMI | CTAG | 3 cut(s) | 545, 746, 1176 |
| SstI | GAGCTC | 1 cut(s) | 1208 |
| StyD4I | CCNGG | 6 cut(s) | 100, 165, 225, 303, 833, 1239 |
| StyI | CCWWGG | 3 cut(s) | 138, 624, 1191 |
| TaaI | ACNGT | 3 cut(s) | 211, 256, 478 |
| TaiI | ACGT | 2 cut(s) | 85, 1013 |
| TaqI | TCGA | 4 cut(s) | 30, 213, 329, 1155 |
| TasI | AATT | 1 cut(s) | 160 |
| TatI | WGTACW | 1 cut(s) | 507 |
| TauI | GCSGC | 6 cut(s) | 197, 319, 413, 657, 752, 858 |
| TfiI | GAWTC | 5 cut(s) | 92, 785, 878, 1109, 1294 |
| Tru1I | TTAA | 3 cut(s) | 72, 885, 1245 |
| Tru9I | TTAA | 3 cut(s) | 72, 885, 1245 |
| TscAI | CASTG | 3 cut(s) | 214, 1038, 1084 |
| TseFI | GTSAC | 3 cut(s) | 472, 1195, 1235 |
| TseI | GCWGC | 6 cut(s) | 182, 199, 466, 1021, 1166, 1280 |
| Tsp45I | GTSAC | 3 cut(s) | 472, 1195, 1235 |
| TspDTI | ATGAA | 3 cut(s) | 840, 1122, 1150 |
| TspGWI | ACGGA | 1 cut(s) | 987 |
| TspRI | CASTG | 3 cut(s) | 214, 1038, 1084 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 310, 981 |
| Vha464I | CTTAAG | 1 cut(s) | 71 |
| VpaK11BI | GGWCC | 4 cut(s) | 142, 739, 952, 1097 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 917 |
| XapI | RAATTY | 1 cut(s) | 160 |
| XbaI | TCTAGA | 1 cut(s) | 544 |
| XceI | RCATGY | 2 cut(s) | 178, 323 |
| XspI | CTAG | 3 cut(s) | 545, 746, 1176 |