No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 142, 1054 |
| Acc36I | ACCTGC | 1 cut(s) | 998 |
| Acc65I | GGTACC | 1 cut(s) | 1133 |
| AccB1I | GGYRCC | 3 cut(s) | 878, 914, 1133 |
| AccBSI | CCGCTC | 3 cut(s) | 316, 556, 685 |
| AccII | CGCG | 5 cut(s) | 155, 376, 645, 806, 1193 |
| AcoI | YGGCCR | 3 cut(s) | 612, 744, 807 |
| AcuI | CTGAAG | 2 cut(s) | 388, 810 |
| AcvI | CACGTG | 1 cut(s) | 1097 |
| AcyI | GRCGYC | 3 cut(s) | 458, 879, 915 |
| AfaI | GTAC | 3 cut(s) | 799, 1012, 1135 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 102, 478, 738, 1007, 1052, 1087 |
| AflII | CTTAAG | 1 cut(s) | 845 |
| AflIII | ACRYGT | 1 cut(s) | 643 |
| AgsI | TTSAA | 5 cut(s) | 38, 821, 829, 904, 1208 |
| AleI | CACNNNNGTG | 1 cut(s) | 15 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 305, 337 |
| AluBI | AGCT | 9 cut(s) | 271, 408, 487, 604, 757, 788, 844, 1004, 1160 |
| AluI | AGCT | 9 cut(s) | 271, 408, 487, 604, 757, 788, 844, 1004, 1160 |
| Alw26I | GTCTC | 3 cut(s) | 512, 652, 974 |
| Ama87I | CYCGRG | 4 cut(s) | 882, 1018, 1122, 1161 |
| AoxI | GGCC | 7 cut(s) | 186, 204, 612, 744, 807, 885, 1036 |
| ApaI | GGGCCC | 1 cut(s) | 190 |
| ApeKI | GCWGC | 9 cut(s) | 215, 466, 676, 721, 757, 959, 1004, 1023, 1211 |
| Asp718I | GGTACC | 1 cut(s) | 1133 |
| AspLEI | GCGC | 8 cut(s) | 118, 132, 256, 378, 881, 917, 1000, 1195 |
| AspS9I | GGNCC | 9 cut(s) | 20, 93, 186, 187, 205, 885, 1037, 1056, 1167 |
| AsuC2I | CCSGG | 5 cut(s) | 472, 883, 884, 1123, 1124 |
| AsuHPI | GGTGA | 3 cut(s) | 97, 390, 779 |
| AsuII | TTCGAA | 1 cut(s) | 221 |
| AvaI | CYCGRG | 4 cut(s) | 882, 1018, 1122, 1161 |
| AvaII | GGWCC | 4 cut(s) | 20, 93, 1056, 1167 |
| BaeGI | GKGCMC | 1 cut(s) | 190 |
| BanI | GGYRCC | 3 cut(s) | 878, 914, 1133 |
| BanII | GRGCYC | 2 cut(s) | 190, 1146 |
| BbrPI | CACGTG | 1 cut(s) | 1097 |
| BbsI | GAAGAC | 3 cut(s) | 138, 230, 1070 |
| BbvCI | CCTCAGC | 1 cut(s) | 488 |
| BbvI | GCAGC | 9 cut(s) | 202, 478, 663, 733, 744, 946, 991, 1035, 1223 |
| BccI | CCATC | 4 cut(s) | 339, 623, 736, 1124 |
| BceAI | ACGGC | 5 cut(s) | 153, 177, 245, 399, 613 |
| BcnI | CCSGG | 5 cut(s) | 472, 883, 884, 1123, 1124 |
| BcoDI | GTCTC | 3 cut(s) | 512, 652, 974 |
| BfaI | CTAG | 2 cut(s) | 77, 272 |
| BfmI | CTRYAG | 2 cut(s) | 677, 1005 |
| BfoI | RGCGCY | 4 cut(s) | 119, 133, 882, 918 |
| BfrI | CTTAAG | 1 cut(s) | 845 |
| BfuAI | ACCTGC | 1 cut(s) | 998 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 212 |
| Bme1390I | CCNGG | 5 cut(s) | 472, 883, 884, 1123, 1124 |
| Bme18I | GGWCC | 4 cut(s) | 20, 93, 1056, 1167 |
| BmeT110I | CYCGRG | 4 cut(s) | 882, 1018, 1122, 1161 |
| BmgT120I | GGNCC | 9 cut(s) | 20, 93, 186, 187, 205, 885, 1037, 1056, 1167 |
| BmrFI | CCNGG | 5 cut(s) | 472, 883, 884, 1123, 1124 |
| BmrI | ACTGGG | 1 cut(s) | 1143 |
| BmsI | GCATC | 1 cut(s) | 241 |
| BmuI | ACTGGG | 1 cut(s) | 1143 |
| BoxI | GACNNNNGTC | 1 cut(s) | 986 |
| BpiI | GAAGAC | 3 cut(s) | 138, 230, 1070 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 260, 292 |
| BpmI | CTGGAG | 3 cut(s) | 140, 494, 1130 |
| Bpu10I | CCTNAGC | 2 cut(s) | 488, 696 |
| Bpu14I | TTCGAA | 1 cut(s) | 221 |
| BpuMI | CCSGG | 5 cut(s) | 472, 883, 884, 1123, 1124 |
| BsaAI | YACGTR | 1 cut(s) | 1097 |
| BsaHI | GRCGYC | 3 cut(s) | 458, 879, 915 |
| BsaI | GGTCTC | 1 cut(s) | 512 |
| BsaJI | CCNNGG | 6 cut(s) | 470, 595, 804, 861, 882, 1122 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 156, 186, 305, 335 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 102, 478, 738, 1007, 1052, 1087 |
| Bse118I | RCCGGY | 2 cut(s) | 40, 955 |
| Bse1I | ACTGG | 1 cut(s) | 1149 |
| BseDI | CCNNGG | 6 cut(s) | 470, 595, 804, 861, 882, 1122 |
| BseGI | GGATG | 3 cut(s) | 747, 1042, 1135 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 102, 478, 738, 1007, 1052, 1087 |
| BseMII | CTCAG | 4 cut(s) | 38, 132, 479, 773 |
| BseNI | ACTGG | 1 cut(s) | 1149 |
| BseSI | GKGCMC | 1 cut(s) | 190 |
| BseX3I | CGGCCG | 1 cut(s) | 807 |
| BseXI | GCAGC | 9 cut(s) | 202, 478, 663, 733, 744, 946, 991, 1035, 1223 |
| BseYI | CCCAGC | 4 cut(s) | 101, 346, 760, 1071 |
| BsgI | GTGCAG | 1 cut(s) | 740 |
| Bsh1236I | CGCG | 5 cut(s) | 155, 376, 645, 806, 1193 |
| Bsh1285I | CGRYCG | 3 cut(s) | 810, 1187, 1191 |
| BshFI | GGCC | 7 cut(s) | 188, 206, 614, 746, 809, 887, 1038 |
| BshNI | GGYRCC | 3 cut(s) | 878, 914, 1133 |
| BsiEI | CGRYCG | 3 cut(s) | 810, 1187, 1191 |
| BsiHKCI | CYCGRG | 4 cut(s) | 882, 1018, 1122, 1161 |
| BsiSI | CCGG | 8 cut(s) | 41, 133, 472, 883, 956, 977, 1101, 1123 |
| BslFI | GGGAC | 1 cut(s) | 1042 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 102, 478, 738, 1007, 1052, 1087 |
| BsmAI | GTCTC | 3 cut(s) | 512, 652, 974 |
| BsmBI | CGTCTC | 1 cut(s) | 652 |
| BsmFI | GGGAC | 1 cut(s) | 1042 |
| BsnI | GGCC | 7 cut(s) | 188, 206, 614, 746, 809, 887, 1038 |
| Bso31I | GGTCTC | 1 cut(s) | 512 |
| BsoBI | CYCGRG | 4 cut(s) | 882, 1018, 1122, 1161 |
| Bsp119I | TTCGAA | 1 cut(s) | 221 |
| Bsp120I | GGGCCC | 1 cut(s) | 186 |
| Bsp1286I | GDGCHC | 2 cut(s) | 190, 1146 |
| Bsp143I | GATC | 3 cut(s) | 666, 1171, 1184 |
| BspANI | GGCC | 7 cut(s) | 188, 206, 614, 746, 809, 887, 1038 |
| BspCNI | CTCAG | 4 cut(s) | 37, 133, 480, 774 |
| BspFNI | CGCG | 5 cut(s) | 155, 376, 645, 806, 1193 |
| BspMAI | CTGCAG | 2 cut(s) | 681, 1009 |
| BspMI | ACCTGC | 1 cut(s) | 998 |
| BspT104I | TTCGAA | 1 cut(s) | 221 |
| BspT107I | GGYRCC | 3 cut(s) | 878, 914, 1133 |
| BspTI | CTTAAG | 1 cut(s) | 845 |
| BspTNI | GGTCTC | 1 cut(s) | 512 |
| BsrBI | CCGCTC | 3 cut(s) | 316, 556, 685 |
| BsrFI | RCCGGY | 2 cut(s) | 40, 955 |
| BsrI | ACTGG | 1 cut(s) | 1149 |
| BssAI | RCCGGY | 2 cut(s) | 40, 955 |
| BssECI | CCNNGG | 6 cut(s) | 470, 595, 804, 861, 882, 1122 |
| BssMI | GATC | 3 cut(s) | 666, 1171, 1184 |
| BssNI | GRCGYC | 3 cut(s) | 458, 879, 915 |
| Bst4CI | ACNGT | 1 cut(s) | 297 |
| Bst6I | CTCTTC | 2 cut(s) | 153, 332 |
| BstACI | GRCGYC | 3 cut(s) | 458, 879, 915 |
| BstAFI | CTTAAG | 1 cut(s) | 845 |
| BstBAI | YACGTR | 1 cut(s) | 1097 |
| BstBI | TTCGAA | 1 cut(s) | 221 |
| BstDEI | CTNAG | 5 cut(s) | 24, 141, 488, 696, 782 |
| BstDSI | CCRYGG | 2 cut(s) | 595, 804 |
| BstEII | GGTNACC | 1 cut(s) | 772 |
| BstF5I | GGATG | 3 cut(s) | 747, 1042, 1135 |
| BstFNI | CGCG | 5 cut(s) | 155, 376, 645, 806, 1193 |
| BstH2I | RGCGCY | 4 cut(s) | 119, 133, 882, 918 |
| BstHHI | GCGC | 8 cut(s) | 118, 132, 256, 378, 881, 917, 1000, 1195 |
| BstKTI | GATC | 3 cut(s) | 669, 1174, 1187 |
| BstMAI | GTCTC | 3 cut(s) | 512, 652, 974 |
| BstMBI | GATC | 3 cut(s) | 666, 1171, 1184 |
| BstMCI | CGRYCG | 3 cut(s) | 810, 1187, 1191 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 212, 277, 290, 682, 809, 872, 956, 1004 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 986 |
| BstPI | GGTNACC | 1 cut(s) | 772 |
| BstSCI | CCNGG | 5 cut(s) | 470, 881, 882, 1121, 1122 |
| BstSFI | CTRYAG | 2 cut(s) | 677, 1005 |
| BstSLI | GKGCMC | 1 cut(s) | 190 |
| BstUI | CGCG | 5 cut(s) | 155, 376, 645, 806, 1193 |
| BstV1I | GCAGC | 9 cut(s) | 202, 478, 663, 733, 744, 946, 991, 1035, 1223 |
| BstV2I | GAAGAC | 3 cut(s) | 138, 230, 1070 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 17, 622 |
| BstZI | CGGCCG | 1 cut(s) | 807 |
| BsuRI | GGCC | 7 cut(s) | 188, 206, 614, 746, 809, 887, 1038 |
| BtgI | CCRYGG | 2 cut(s) | 595, 804 |
| BtsCI | GGATG | 3 cut(s) | 747, 1042, 1135 |
| BtsIMutI | CAGTG | 1 cut(s) | 854 |
| BveI | ACCTGC | 1 cut(s) | 998 |
| CciNI | GCGGCCGC | 1 cut(s) | 807 |
| CfoI | GCGC | 8 cut(s) | 118, 132, 256, 378, 881, 917, 1000, 1195 |
| Cfr10I | RCCGGY | 2 cut(s) | 40, 955 |
| Cfr13I | GGNCC | 9 cut(s) | 20, 93, 186, 187, 205, 885, 1037, 1056, 1167 |
| Cfr42I | CCGCGG | 1 cut(s) | 807 |
| Cfr9I | CCCGGG | 2 cut(s) | 882, 1122 |
| CseI | GACGC | 4 cut(s) | 161, 447, 634, 956 |
| Csp6I | GTAC | 3 cut(s) | 798, 1011, 1134 |
| CviAII | CATG | 1 cut(s) | 440 |
| CviQI | GTAC | 3 cut(s) | 798, 1011, 1134 |
| DdeI | CTNAG | 5 cut(s) | 24, 141, 488, 696, 782 |
| DinI | GGCGCC | 2 cut(s) | 880, 916 |
| DpnI | GATC | 3 cut(s) | 668, 1173, 1186 |
| DpnII | GATC | 3 cut(s) | 666, 1171, 1184 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 142, 1054 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 142, 1054 |
| EaeI | YGGCCR | 3 cut(s) | 612, 744, 807 |
| EagI | CGGCCG | 1 cut(s) | 807 |
| Eam1104I | CTCTTC | 2 cut(s) | 153, 332 |
| EarI | CTCTTC | 2 cut(s) | 153, 332 |
| EciI | GGCGGA | 5 cut(s) | 59, 539, 587, 721, 1142 |
| EclXI | CGGCCG | 1 cut(s) | 807 |
| Eco24I | GRGCYC | 2 cut(s) | 190, 1146 |
| Eco31I | GGTCTC | 1 cut(s) | 512 |
| Eco47I | GGWCC | 4 cut(s) | 20, 93, 1056, 1167 |
| Eco52I | CGGCCG | 1 cut(s) | 807 |
| Eco57I | CTGAAG | 2 cut(s) | 388, 810 |
| Eco72I | CACGTG | 1 cut(s) | 1097 |
| Eco88I | CYCGRG | 4 cut(s) | 882, 1018, 1122, 1161 |
| Eco91I | GGTNACC | 1 cut(s) | 772 |
| EcoO109I | RGGNCCY | 2 cut(s) | 186, 1056 |
| EcoO65I | GGTNACC | 1 cut(s) | 772 |
| EcoT38I | GRGCYC | 2 cut(s) | 190, 1146 |
| EgeI | GGCGCC | 2 cut(s) | 880, 916 |
| EheI | GGCGCC | 2 cut(s) | 880, 916 |
| Esp3I | CGTCTC | 1 cut(s) | 652 |
| FaeI | CATG | 1 cut(s) | 443 |
| FaqI | GGGAC | 1 cut(s) | 1042 |
| FatI | CATG | 1 cut(s) | 439 |
| FauI | CCCGC | 9 cut(s) | 197, 265, 309, 358, 424, 549, 717, 785, 1019 |
| FokI | GGATG | 3 cut(s) | 754, 1029, 1142 |
| FriOI | GRGCYC | 2 cut(s) | 190, 1146 |
| FspBI | CTAG | 2 cut(s) | 77, 272 |
| GlaI | GCGC | 8 cut(s) | 117, 131, 255, 377, 880, 916, 999, 1194 |
| GsaI | CCCAGC | 4 cut(s) | 105, 350, 764, 1075 |
| GsuI | CTGGAG | 3 cut(s) | 140, 494, 1130 |
| HaeII | RGCGCY | 4 cut(s) | 119, 133, 882, 918 |
| HaeIII | GGCC | 7 cut(s) | 188, 206, 614, 746, 809, 887, 1038 |
| HapII | CCGG | 8 cut(s) | 41, 133, 472, 883, 956, 977, 1101, 1123 |
| HgaI | GACGC | 4 cut(s) | 161, 447, 634, 956 |
| HhaI | GCGC | 8 cut(s) | 118, 132, 256, 378, 881, 917, 1000, 1195 |
| Hin1I | GRCGYC | 3 cut(s) | 458, 879, 915 |
| Hin1II | CATG | 1 cut(s) | 443 |
| Hin6I | GCGC | 8 cut(s) | 116, 130, 254, 376, 879, 915, 998, 1193 |
| HinP1I | GCGC | 8 cut(s) | 116, 130, 254, 376, 879, 915, 998, 1193 |
| HindIII | AAGCTT | 1 cut(s) | 602 |
| HinfI | GANTC | 4 cut(s) | 143, 275, 728, 1066 |
| HpaII | CCGG | 8 cut(s) | 41, 133, 472, 883, 956, 977, 1101, 1123 |
| HphI | GGTGA | 3 cut(s) | 97, 390, 779 |
| Hpy188I | TCNGA | 8 cut(s) | 27, 151, 198, 503, 1047, 1087, 1155, 1171 |
| Hpy188III | TCNNGA | 2 cut(s) | 511, 1109 |
| Hpy99I | CGWCG | 4 cut(s) | 385, 463, 1051, 1087 |
| HpyAV | CCTTC | 8 cut(s) | 22, 196, 302, 412, 785, 865, 904, 1046 |
| HpyCH4III | ACNGT | 1 cut(s) | 297 |
| HpyCH4IV | ACGT | 1 cut(s) | 1096 |
| HpyCH4V | TGCA | 5 cut(s) | 293, 439, 679, 721, 1007 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 212, 277, 290, 682, 809, 872, 956, 1004 |
| HpyF3I | CTNAG | 5 cut(s) | 24, 141, 488, 696, 782 |
| HpySE526I | ACGT | 1 cut(s) | 1096 |
| Hsp92I | GRCGYC | 3 cut(s) | 458, 879, 915 |
| Hsp92II | CATG | 1 cut(s) | 443 |
| HspAI | GCGC | 8 cut(s) | 116, 130, 254, 376, 879, 915, 998, 1193 |
| KasI | GGCGCC | 2 cut(s) | 878, 914 |
| KflI | GGGWCCC | 1 cut(s) | 1056 |
| KpnI | GGTACC | 1 cut(s) | 1137 |
| KroI | GCCGGC | 1 cut(s) | 955 |
| KroNI | GCCGGC | 1 cut(s) | 957 |
| KspI | CCGCGG | 1 cut(s) | 807 |
| Kzo9I | GATC | 3 cut(s) | 666, 1171, 1184 |
| LmnI | GCTCC | 3 cut(s) | 313, 658, 682 |
| Lsp1109I | GCAGC | 9 cut(s) | 202, 478, 663, 733, 744, 946, 991, 1035, 1223 |
| LweI | GCATC | 1 cut(s) | 241 |
| MaeI | CTAG | 2 cut(s) | 77, 272 |
| MaeII | ACGT | 1 cut(s) | 1096 |
| MaeIII | GTNAC | 6 cut(s) | 58, 85, 301, 578, 772, 1092 |
| MalI | GATC | 3 cut(s) | 668, 1173, 1186 |
| MbiI | CCGCTC | 3 cut(s) | 316, 556, 685 |
| MboI | GATC | 3 cut(s) | 666, 1171, 1184 |
| MboII | GAAGA | 8 cut(s) | 59, 138, 170, 211, 235, 319, 506, 1075 |
| MfeI | CAATTG | 1 cut(s) | 387 |
| MhlI | GDGCHC | 2 cut(s) | 190, 1146 |
| MluCI | AATT | 1 cut(s) | 387 |
| MluI | ACGCGT | 1 cut(s) | 643 |
| Mly113I | GGCGCC | 2 cut(s) | 879, 915 |
| MlyI | GAGTC | 4 cut(s) | 152, 269, 737, 1060 |
| MmeI | TCCRAC | 4 cut(s) | 636, 1065, 1070, 1178 |
| MroNI | GCCGGC | 1 cut(s) | 955 |
| MseI | TTAA | 2 cut(s) | 606, 846 |
| MslI | CAYNNNNRTG | 2 cut(s) | 15, 716 |
| MspA1I | CMGCKG | 7 cut(s) | 101, 724, 806, 1004, 1026, 1214, 1233 |
| MspCI | CTTAAG | 1 cut(s) | 845 |
| MspI | CCGG | 8 cut(s) | 41, 133, 472, 883, 956, 977, 1101, 1123 |
| MspR9I | CCNGG | 5 cut(s) | 472, 883, 884, 1123, 1124 |
| MunI | CAATTG | 1 cut(s) | 387 |
| MvnI | CGCG | 5 cut(s) | 155, 376, 645, 806, 1193 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 212, 277, 290, 682, 809, 872, 956, 1004 |
| NaeI | GCCGGC | 1 cut(s) | 957 |
| NarI | GGCGCC | 2 cut(s) | 879, 915 |
| NciI | CCSGG | 5 cut(s) | 472, 883, 884, 1123, 1124 |
| NdeII | GATC | 3 cut(s) | 666, 1171, 1184 |
| NgoMIV | GCCGGC | 1 cut(s) | 955 |
| NlaIII | CATG | 1 cut(s) | 443 |
| NmeAIII | GCCGAG | 1 cut(s) | 310 |
| NmuCI | GTSAC | 5 cut(s) | 58, 85, 301, 578, 1092 |
| NotI | GCGGCCGC | 1 cut(s) | 807 |
| NspV | TTCGAA | 1 cut(s) | 221 |
| OliI | CACNNNNGTG | 1 cut(s) | 15 |
| PaeR7I | CTCGAG | 1 cut(s) | 1161 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 858 |
| PdiI | GCCGGC | 1 cut(s) | 957 |
| PflFI | GACNNNGTC | 2 cut(s) | 926, 1081 |
| Ple19I | CGATCG | 1 cut(s) | 1187 |
| PleI | GAGTC | 4 cut(s) | 151, 269, 736, 1060 |
| PluTI | GGCGCC | 2 cut(s) | 882, 918 |
| PmaCI | CACGTG | 1 cut(s) | 1097 |
| PmlI | CACGTG | 1 cut(s) | 1097 |
| PpsI | GAGTC | 4 cut(s) | 151, 269, 736, 1060 |
| Ppu21I | YACGTR | 1 cut(s) | 1097 |
| PpuMI | RGGWCCY | 1 cut(s) | 1056 |
| PshAI | GACNNNNGTC | 1 cut(s) | 986 |
| Psp5II | RGGWCCY | 1 cut(s) | 1056 |
| PspCI | CACGTG | 1 cut(s) | 1097 |
| PspEI | GGTNACC | 1 cut(s) | 772 |
| PspFI | CCCAGC | 4 cut(s) | 101, 346, 760, 1071 |
| PspOMI | GGGCCC | 1 cut(s) | 186 |
| PspPI | GGNCC | 9 cut(s) | 20, 93, 186, 187, 205, 885, 1037, 1056, 1167 |
| PspPPI | RGGWCCY | 1 cut(s) | 1056 |
| PspXI | VCTCGAGB | 1 cut(s) | 1161 |
| PstI | CTGCAG | 2 cut(s) | 681, 1009 |
| PsyI | GACNNNGTC | 2 cut(s) | 926, 1081 |
| PvuI | CGATCG | 1 cut(s) | 1187 |
| PvuII | CAGCTG | 1 cut(s) | 1004 |
| RsaI | GTAC | 3 cut(s) | 799, 1012, 1135 |
| RsaNI | GTAC | 3 cut(s) | 798, 1011, 1134 |
| RseI | CAYNNNNRTG | 2 cut(s) | 15, 716 |
| SacII | CCGCGG | 1 cut(s) | 807 |
| SaqAI | TTAA | 2 cut(s) | 606, 846 |
| Sau3AI | GATC | 3 cut(s) | 666, 1171, 1184 |
| Sau96I | GGNCC | 9 cut(s) | 20, 93, 186, 187, 205, 885, 1037, 1056, 1167 |
| SchI | GAGTC | 4 cut(s) | 152, 269, 737, 1060 |
| ScrFI | CCNGG | 5 cut(s) | 472, 883, 884, 1123, 1124 |
| SduI | GDGCHC | 2 cut(s) | 190, 1146 |
| SfaNI | GCATC | 1 cut(s) | 241 |
| SfcI | CTRYAG | 2 cut(s) | 677, 1005 |
| SfoI | GGCGCC | 2 cut(s) | 880, 916 |
| Sfr274I | CTCGAG | 1 cut(s) | 1161 |
| Sfr303I | CCGCGG | 1 cut(s) | 807 |
| SfuI | TTCGAA | 1 cut(s) | 221 |
| SgrBI | CCGCGG | 1 cut(s) | 807 |
| SinI | GGWCC | 4 cut(s) | 20, 93, 1056, 1167 |
| SlaI | CTCGAG | 1 cut(s) | 1161 |
| SmaI | CCCGGG | 2 cut(s) | 884, 1124 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 15, 716 |
| SmlI | CTYRAG | 2 cut(s) | 845, 1161 |
| SmoI | CTYRAG | 2 cut(s) | 845, 1161 |
| SrfI | GCCCGGGC | 1 cut(s) | 884 |
| Sse9I | AATT | 1 cut(s) | 387 |
| SspDI | GGCGCC | 2 cut(s) | 878, 914 |
| SspMI | CTAG | 2 cut(s) | 77, 272 |
| StyD4I | CCNGG | 5 cut(s) | 470, 881, 882, 1121, 1122 |
| TaaI | ACNGT | 1 cut(s) | 297 |
| TaiI | ACGT | 1 cut(s) | 1099 |
| TaqI | TCGA | 4 cut(s) | 221, 461, 1162, 1221 |
| TaqII | GACCGA | 2 cut(s) | 875, 1177 |
| TasI | AATT | 1 cut(s) | 387 |
| Tru1I | TTAA | 2 cut(s) | 606, 846 |
| Tru9I | TTAA | 2 cut(s) | 606, 846 |
| TscAI | CASTG | 1 cut(s) | 861 |
| TseFI | GTSAC | 5 cut(s) | 58, 85, 301, 578, 1092 |
| TseI | GCWGC | 9 cut(s) | 215, 466, 676, 721, 757, 959, 1004, 1023, 1211 |
| Tsp45I | GTSAC | 5 cut(s) | 58, 85, 301, 578, 1092 |
| TspDTI | ATGAA | 2 cut(s) | 654, 767 |
| TspGWI | ACGGA | 2 cut(s) | 560, 1064 |
| TspMI | CCCGGG | 2 cut(s) | 882, 1122 |
| TspRI | CASTG | 1 cut(s) | 861 |
| Tth111I | GACNNNGTC | 2 cut(s) | 926, 1081 |
| Vha464I | CTTAAG | 1 cut(s) | 845 |
| VpaK11BI | GGWCC | 4 cut(s) | 20, 93, 1056, 1167 |
| XhoI | CTCGAG | 1 cut(s) | 1161 |
| XmaI | CCCGGG | 2 cut(s) | 882, 1122 |
| XspI | CTAG | 2 cut(s) | 77, 272 |