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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 404 |
| AccB1I | GGYRCC | 2 cut(s) | 404, 771 |
| AccBSI | CCGCTC | 2 cut(s) | 694, 769 |
| AccII | CGCG | 3 cut(s) | 129, 476, 671 |
| AclWI | GGATC | 1 cut(s) | 706 |
| AcoI | YGGCCR | 1 cut(s) | 46 |
| AcsI | RAATTY | 1 cut(s) | 509 |
| AcuI | CTGAAG | 2 cut(s) | 294, 722 |
| AcyI | GRCGYC | 2 cut(s) | 447, 772 |
| AfaI | GTAC | 3 cut(s) | 42, 175, 406 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 150, 151, 297, 529, 685, 781 |
| AgsI | TTSAA | 1 cut(s) | 605 |
| AjnI | CCWGG | 1 cut(s) | 244 |
| AleI | CACNNNNGTG | 1 cut(s) | 549 |
| AluBI | AGCT | 6 cut(s) | 260, 375, 621, 658, 700, 733 |
| AluI | AGCT | 6 cut(s) | 260, 375, 621, 658, 700, 733 |
| Alw26I | GTCTC | 2 cut(s) | 292, 725 |
| AlwI | GGATC | 1 cut(s) | 706 |
| Ama87I | CYCGRG | 4 cut(s) | 120, 150, 524, 684 |
| AoxI | GGCC | 5 cut(s) | 46, 323, 389, 489, 741 |
| ApaI | GGGCCC | 1 cut(s) | 493 |
| ApeKI | GCWGC | 4 cut(s) | 217, 238, 649, 697 |
| ApoI | RAATTY | 1 cut(s) | 509 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 175, 279 |
| Asp718I | GGTACC | 1 cut(s) | 404 |
| AspLEI | GCGC | 4 cut(s) | 134, 208, 254, 774 |
| AspS9I | GGNCC | 5 cut(s) | 140, 324, 381, 489, 490 |
| AsuHPI | GGTGA | 2 cut(s) | 72, 148 |
| AvaI | CYCGRG | 4 cut(s) | 120, 150, 524, 684 |
| AvaII | GGWCC | 2 cut(s) | 140, 381 |
| BaeGI | GKGCMC | 1 cut(s) | 493 |
| BanI | GGYRCC | 2 cut(s) | 404, 771 |
| BanII | GRGCYC | 1 cut(s) | 493 |
| BbvI | GCAGC | 4 cut(s) | 229, 250, 636, 709 |
| BciT130I | CCWGG | 1 cut(s) | 246 |
| BcoDI | GTCTC | 2 cut(s) | 292, 725 |
| BfmI | CTRYAG | 3 cut(s) | 19, 422, 642 |
| BfoI | RGCGCY | 4 cut(s) | 135, 209, 255, 775 |
| BisI | GCNGC | 9 cut(s) | 130, 218, 239, 242, 399, 650, 695, 698, 770 |
| BlpI | GCTNAGC | 1 cut(s) | 622 |
| BlsI | GCNGC | 9 cut(s) | 131, 219, 240, 243, 400, 651, 696, 699, 771 |
| BmcAI | AGTACT | 2 cut(s) | 42, 175 |
| Bme1390I | CCNGG | 1 cut(s) | 246 |
| Bme18I | GGWCC | 2 cut(s) | 140, 381 |
| BmeT110I | CYCGRG | 4 cut(s) | 120, 150, 524, 684 |
| BmgT120I | GGNCC | 5 cut(s) | 140, 324, 381, 489, 490 |
| BmiI | GGNNCC | 7 cut(s) | 141, 142, 302, 382, 406, 491, 773 |
| BmrFI | CCNGG | 1 cut(s) | 246 |
| BmsI | GCATC | 1 cut(s) | 492 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 622 |
| BpuEI | CTTGAG | 2 cut(s) | 254, 406 |
| BsaHI | GRCGYC | 2 cut(s) | 447, 772 |
| BsaI | GGTCTC | 1 cut(s) | 725 |
| BsaJI | CCNNGG | 5 cut(s) | 245, 291, 327, 683, 715 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 150, 151, 297, 529, 685, 781 |
| Bse118I | RCCGGY | 3 cut(s) | 48, 365, 401 |
| Bse1I | ACTGG | 2 cut(s) | 196, 349 |
| BseBI | CCWGG | 1 cut(s) | 246 |
| BseDI | CCNNGG | 5 cut(s) | 245, 291, 327, 683, 715 |
| BseGI | GGATG | 2 cut(s) | 67, 583 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 150, 151, 297, 529, 685, 781 |
| BseMII | CTCAG | 2 cut(s) | 363, 636 |
| BseNI | ACTGG | 2 cut(s) | 196, 349 |
| BseSI | GKGCMC | 1 cut(s) | 493 |
| BseXI | GCAGC | 4 cut(s) | 229, 250, 636, 709 |
| BseYI | CCCAGC | 2 cut(s) | 375, 733 |
| Bsh1236I | CGCG | 3 cut(s) | 129, 476, 671 |
| BshFI | GGCC | 5 cut(s) | 48, 325, 391, 491, 743 |
| BshNI | GGYRCC | 2 cut(s) | 404, 771 |
| BsiHKCI | CYCGRG | 4 cut(s) | 120, 150, 524, 684 |
| BsiSI | CCGG | 3 cut(s) | 49, 366, 402 |
| BslFI | GGGAC | 2 cut(s) | 153, 394 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 150, 151, 297, 529, 685, 781 |
| BsmAI | GTCTC | 2 cut(s) | 292, 725 |
| BsmFI | GGGAC | 2 cut(s) | 153, 394 |
| BsnI | GGCC | 5 cut(s) | 48, 325, 391, 491, 743 |
| Bso31I | GGTCTC | 1 cut(s) | 725 |
| BsoBI | CYCGRG | 4 cut(s) | 120, 150, 524, 684 |
| Bsp120I | GGGCCC | 1 cut(s) | 489 |
| Bsp1286I | GDGCHC | 1 cut(s) | 493 |
| Bsp143I | GATC | 2 cut(s) | 352, 711 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 622 |
| BspANI | GGCC | 5 cut(s) | 48, 325, 391, 491, 743 |
| BspCNI | CTCAG | 2 cut(s) | 362, 635 |
| BspFNI | CGCG | 3 cut(s) | 129, 476, 671 |
| BspLI | GGNNCC | 7 cut(s) | 141, 142, 302, 382, 406, 491, 773 |
| BspMAI | CTGCAG | 1 cut(s) | 426 |
| BspPI | GGATC | 1 cut(s) | 706 |
| BspT107I | GGYRCC | 2 cut(s) | 404, 771 |
| BspTNI | GGTCTC | 1 cut(s) | 725 |
| BsrBI | CCGCTC | 2 cut(s) | 694, 769 |
| BsrFI | RCCGGY | 3 cut(s) | 48, 365, 401 |
| BsrI | ACTGG | 2 cut(s) | 196, 349 |
| BssAI | RCCGGY | 3 cut(s) | 48, 365, 401 |
| BssECI | CCNNGG | 5 cut(s) | 245, 291, 327, 683, 715 |
| BssMI | GATC | 2 cut(s) | 352, 711 |
| BssNI | GRCGYC | 2 cut(s) | 447, 772 |
| BssT1I | CCWWGG | 2 cut(s) | 291, 715 |
| Bst2UI | CCWGG | 1 cut(s) | 246 |
| Bst4CI | ACNGT | 1 cut(s) | 609 |
| Bst6I | CTCTTC | 2 cut(s) | 545, 702 |
| BstACI | GRCGYC | 2 cut(s) | 447, 772 |
| BstC8I | GCNNGC | 3 cut(s) | 103, 323, 493 |
| BstDEI | CTNAG | 3 cut(s) | 349, 622, 780 |
| BstDSI | CCRYGG | 1 cut(s) | 327 |
| BstEII | GGTNACC | 2 cut(s) | 78, 154 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 779 |
| BstF5I | GGATG | 2 cut(s) | 67, 583 |
| BstFNI | CGCG | 3 cut(s) | 129, 476, 671 |
| BstH2I | RGCGCY | 4 cut(s) | 135, 209, 255, 775 |
| BstHHI | GCGC | 4 cut(s) | 134, 208, 254, 774 |
| BstKTI | GATC | 2 cut(s) | 355, 714 |
| BstMAI | GTCTC | 2 cut(s) | 292, 725 |
| BstMBI | GATC | 2 cut(s) | 352, 711 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 214, 354, 655 |
| BstNI | CCWGG | 1 cut(s) | 246 |
| BstPI | GGTNACC | 2 cut(s) | 78, 154 |
| BstSCI | CCNGG | 1 cut(s) | 244 |
| BstSFI | CTRYAG | 3 cut(s) | 19, 422, 642 |
| BstSLI | GKGCMC | 1 cut(s) | 493 |
| BstUI | CGCG | 3 cut(s) | 129, 476, 671 |
| BstV1I | GCAGC | 4 cut(s) | 229, 250, 636, 709 |
| BstX2I | RGATCY | 1 cut(s) | 711 |
| BstYI | RGATCY | 1 cut(s) | 711 |
| BsuRI | GGCC | 5 cut(s) | 48, 325, 391, 491, 743 |
| BtgI | CCRYGG | 1 cut(s) | 327 |
| BtsCI | GGATG | 2 cut(s) | 67, 583 |
| Cac8I | GCNNGC | 3 cut(s) | 103, 323, 493 |
| CfoI | GCGC | 4 cut(s) | 134, 208, 254, 774 |
| Cfr10I | RCCGGY | 3 cut(s) | 48, 365, 401 |
| Cfr13I | GGNCC | 5 cut(s) | 140, 324, 381, 489, 490 |
| CseI | GACGC | 3 cut(s) | 455, 482, 660 |
| Csp6I | GTAC | 3 cut(s) | 41, 174, 405 |
| CviAII | CATG | 4 cut(s) | 284, 411, 497, 653 |
| CviQI | GTAC | 3 cut(s) | 41, 174, 405 |
| DdeI | CTNAG | 3 cut(s) | 349, 622, 780 |
| DinI | GGCGCC | 1 cut(s) | 773 |
| DpnI | GATC | 2 cut(s) | 354, 713 |
| DpnII | GATC | 2 cut(s) | 352, 711 |
| EaeI | YGGCCR | 1 cut(s) | 46 |
| Eam1104I | CTCTTC | 2 cut(s) | 545, 702 |
| EarI | CTCTTC | 2 cut(s) | 545, 702 |
| EciI | GGCGGA | 1 cut(s) | 578 |
| Eco130I | CCWWGG | 2 cut(s) | 291, 715 |
| Eco147I | AGGCCT | 1 cut(s) | 391 |
| Eco24I | GRGCYC | 1 cut(s) | 493 |
| Eco31I | GGTCTC | 1 cut(s) | 725 |
| Eco47I | GGWCC | 2 cut(s) | 140, 381 |
| Eco57I | CTGAAG | 2 cut(s) | 294, 722 |
| Eco88I | CYCGRG | 4 cut(s) | 120, 150, 524, 684 |
| Eco91I | GGTNACC | 2 cut(s) | 78, 154 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 779 |
| EcoO109I | RGGNCCY | 3 cut(s) | 140, 381, 489 |
| EcoO65I | GGTNACC | 2 cut(s) | 78, 154 |
| EcoRI | GAATTC | 1 cut(s) | 509 |
| EcoRII | CCWGG | 1 cut(s) | 244 |
| EcoT14I | CCWWGG | 2 cut(s) | 291, 715 |
| EcoT38I | GRGCYC | 1 cut(s) | 493 |
| EgeI | GGCGCC | 1 cut(s) | 773 |
| EheI | GGCGCC | 1 cut(s) | 773 |
| ErhI | CCWWGG | 2 cut(s) | 291, 715 |
| FaeI | CATG | 4 cut(s) | 287, 414, 500, 656 |
| FaiI | YATR | 7 cut(s) | 199, 285, 310, 412, 498, 504, 654 |
| FaqI | GGGAC | 2 cut(s) | 153, 394 |
| FatI | CATG | 4 cut(s) | 283, 410, 496, 652 |
| FauI | CCCGC | 5 cut(s) | 213, 289, 362, 500, 563 |
| Fnu4HI | GCNGC | 9 cut(s) | 130, 218, 239, 242, 399, 650, 695, 698, 770 |
| FokI | GGATG | 2 cut(s) | 74, 590 |
| FriOI | GRGCYC | 1 cut(s) | 493 |
| Fsp4HI | GCNGC | 9 cut(s) | 130, 218, 239, 242, 399, 650, 695, 698, 770 |
| GlaI | GCGC | 4 cut(s) | 133, 207, 253, 773 |
| GluI | GCNGC | 9 cut(s) | 130, 218, 239, 242, 399, 650, 695, 698, 770 |
| GsaI | CCCAGC | 2 cut(s) | 379, 737 |
| HaeII | RGCGCY | 4 cut(s) | 135, 209, 255, 775 |
| HaeIII | GGCC | 5 cut(s) | 48, 325, 391, 491, 743 |
| HapII | CCGG | 3 cut(s) | 49, 366, 402 |
| HgaI | GACGC | 3 cut(s) | 455, 482, 660 |
| HhaI | GCGC | 4 cut(s) | 134, 208, 254, 774 |
| Hin1I | GRCGYC | 2 cut(s) | 447, 772 |
| Hin1II | CATG | 4 cut(s) | 287, 414, 500, 656 |
| Hin6I | GCGC | 4 cut(s) | 132, 206, 252, 772 |
| HinP1I | GCGC | 4 cut(s) | 132, 206, 252, 772 |
| HinfI | GANTC | 1 cut(s) | 269 |
| HpaII | CCGG | 3 cut(s) | 49, 366, 402 |
| HphI | GGTGA | 2 cut(s) | 72, 148 |
| Hpy166II | GTNNAC | 1 cut(s) | 230 |
| Hpy188I | TCNGA | 2 cut(s) | 274, 352 |
| Hpy188III | TCNNGA | 4 cut(s) | 55, 266, 335, 514 |
| Hpy8I | GTNNAC | 1 cut(s) | 230 |
| Hpy99I | CGWCG | 1 cut(s) | 449 |
| HpyAV | CCTTC | 5 cut(s) | 52, 269, 314, 697, 733 |
| HpyCH4III | ACNGT | 1 cut(s) | 609 |
| HpyCH4V | TGCA | 3 cut(s) | 217, 424, 652 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 214, 354, 655 |
| HpyF3I | CTNAG | 3 cut(s) | 349, 622, 780 |
| Hsp92I | GRCGYC | 2 cut(s) | 447, 772 |
| Hsp92II | CATG | 4 cut(s) | 287, 414, 500, 656 |
| HspAI | GCGC | 4 cut(s) | 132, 206, 252, 772 |
| KasI | GGCGCC | 1 cut(s) | 771 |
| KflI | GGGWCCC | 1 cut(s) | 140 |
| KpnI | GGTACC | 1 cut(s) | 408 |
| Kzo9I | GATC | 2 cut(s) | 352, 711 |
| LmnI | GCTCC | 3 cut(s) | 249, 372, 730 |
| Lsp1109I | GCAGC | 4 cut(s) | 229, 250, 636, 709 |
| LweI | GCATC | 1 cut(s) | 492 |
| MaeIII | GTNAC | 4 cut(s) | 23, 78, 154, 164 |
| MalI | GATC | 2 cut(s) | 354, 713 |
| MbiI | CCGCTC | 2 cut(s) | 694, 769 |
| MboI | GATC | 2 cut(s) | 352, 711 |
| MboII | GAAGA | 4 cut(s) | 467, 470, 532, 719 |
| MflI | RGATCY | 1 cut(s) | 711 |
| MhlI | GDGCHC | 1 cut(s) | 493 |
| MluCI | AATT | 1 cut(s) | 509 |
| Mly113I | GGCGCC | 1 cut(s) | 772 |
| MmeI | TCCRAC | 1 cut(s) | 555 |
| MnlI | CCTC | 6 cut(s) | 155, 331, 381, 457, 557, 693 |
| MroXI | GAANNNNTTC | 2 cut(s) | 175, 279 |
| MseI | TTAA | 1 cut(s) | 560 |
| MslI | CAYNNNNRTG | 2 cut(s) | 501, 549 |
| MspA1I | CMGCKG | 2 cut(s) | 220, 700 |
| MspI | CCGG | 3 cut(s) | 49, 366, 402 |
| MspR9I | CCNGG | 1 cut(s) | 246 |
| MvaI | CCWGG | 1 cut(s) | 246 |
| MvnI | CGCG | 3 cut(s) | 129, 476, 671 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 214, 354, 655 |
| NarI | GGCGCC | 1 cut(s) | 772 |
| NdeII | GATC | 2 cut(s) | 352, 711 |
| NlaIII | CATG | 4 cut(s) | 287, 414, 500, 656 |
| NlaIV | GGNNCC | 7 cut(s) | 141, 142, 302, 382, 406, 491, 773 |
| NmuCI | GTSAC | 4 cut(s) | 23, 78, 154, 164 |
| OliI | CACNNNNGTG | 1 cut(s) | 549 |
| PceI | AGGCCT | 1 cut(s) | 391 |
| PdmI | GAANNNNTTC | 2 cut(s) | 175, 279 |
| PfeI | GAWTC | 1 cut(s) | 269 |
| PkrI | GCNGC | 9 cut(s) | 131, 219, 240, 243, 400, 651, 696, 699, 771 |
| PluTI | GGCGCC | 1 cut(s) | 775 |
| PpuMI | RGGWCCY | 2 cut(s) | 140, 381 |
| Psp5II | RGGWCCY | 2 cut(s) | 140, 381 |
| Psp6I | CCWGG | 1 cut(s) | 244 |
| PspEI | GGTNACC | 2 cut(s) | 78, 154 |
| PspFI | CCCAGC | 2 cut(s) | 375, 733 |
| PspGI | CCWGG | 1 cut(s) | 244 |
| PspN4I | GGNNCC | 7 cut(s) | 141, 142, 302, 382, 406, 491, 773 |
| PspOMI | GGGCCC | 1 cut(s) | 489 |
| PspPI | GGNCC | 5 cut(s) | 140, 324, 381, 489, 490 |
| PspPPI | RGGWCCY | 2 cut(s) | 140, 381 |
| PstI | CTGCAG | 1 cut(s) | 426 |
| PsuI | RGATCY | 1 cut(s) | 711 |
| PvuII | CAGCTG | 1 cut(s) | 700 |
| RsaI | GTAC | 3 cut(s) | 42, 175, 406 |
| RsaNI | GTAC | 3 cut(s) | 41, 174, 405 |
| RseI | CAYNNNNRTG | 2 cut(s) | 501, 549 |
| SaqAI | TTAA | 1 cut(s) | 560 |
| SatI | GCNGC | 9 cut(s) | 130, 218, 239, 242, 399, 650, 695, 698, 770 |
| Sau3AI | GATC | 2 cut(s) | 352, 711 |
| Sau96I | GGNCC | 5 cut(s) | 140, 324, 381, 489, 490 |
| ScaI | AGTACT | 2 cut(s) | 42, 175 |
| ScrFI | CCNGG | 1 cut(s) | 246 |
| SduI | GDGCHC | 1 cut(s) | 493 |
| SfaNI | GCATC | 1 cut(s) | 492 |
| SfcI | CTRYAG | 3 cut(s) | 19, 422, 642 |
| SfoI | GGCGCC | 1 cut(s) | 773 |
| SinI | GGWCC | 2 cut(s) | 140, 381 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 501, 549 |
| SmlI | CTYRAG | 2 cut(s) | 233, 385 |
| SmoI | CTYRAG | 2 cut(s) | 233, 385 |
| Sse9I | AATT | 1 cut(s) | 509 |
| SseBI | AGGCCT | 1 cut(s) | 391 |
| SspDI | GGCGCC | 1 cut(s) | 771 |
| StuI | AGGCCT | 1 cut(s) | 391 |
| StyD4I | CCNGG | 1 cut(s) | 244 |
| StyI | CCWWGG | 2 cut(s) | 291, 715 |
| TaaI | ACNGT | 1 cut(s) | 609 |
| TaqI | TCGA | 2 cut(s) | 56, 265 |
| TaqII | GACCGA | 2 cut(s) | 66, 533 |
| TasI | AATT | 1 cut(s) | 509 |
| TatI | WGTACW | 2 cut(s) | 40, 173 |
| TauI | GCSGC | 5 cut(s) | 132, 244, 401, 697, 772 |
| TfiI | GAWTC | 1 cut(s) | 269 |
| Tru1I | TTAA | 1 cut(s) | 560 |
| Tru9I | TTAA | 1 cut(s) | 560 |
| TseFI | GTSAC | 4 cut(s) | 23, 78, 154, 164 |
| TseI | GCWGC | 4 cut(s) | 217, 238, 649, 697 |
| Tsp45I | GTSAC | 4 cut(s) | 23, 78, 154, 164 |
| TspDTI | ATGAA | 3 cut(s) | 272, 468, 522 |
| TspGWI | ACGGA | 1 cut(s) | 183 |
| VpaK11BI | GGWCC | 2 cut(s) | 140, 381 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 779 |
| XapI | RAATTY | 1 cut(s) | 509 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 751 |
| XmnI | GAANNNNTTC | 2 cut(s) | 175, 279 |
| ZrmI | AGTACT | 2 cut(s) | 42, 175 |