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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 309 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 50 |
| Acc65I | GGTACC | 1 cut(s) | 167 |
| AccB1I | GGYRCC | 2 cut(s) | 167, 704 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 205 |
| AclWI | GGATC | 1 cut(s) | 545 |
| AcvI | CACGTG | 1 cut(s) | 711 |
| AfaI | GTAC | 3 cut(s) | 169, 240, 274 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 205, 269, 581 |
| AflIII | ACRYGT | 2 cut(s) | 202, 317 |
| AgeI | ACCGGT | 1 cut(s) | 395 |
| AgsI | TTSAA | 1 cut(s) | 179 |
| AluBI | AGCT | 3 cut(s) | 229, 485, 502 |
| AluI | AGCT | 3 cut(s) | 229, 485, 502 |
| Alw21I | GWGCWC | 1 cut(s) | 231 |
| AlwI | GGATC | 1 cut(s) | 545 |
| Ama87I | CYCGRG | 1 cut(s) | 576 |
| AoxI | GGCC | 1 cut(s) | 256 |
| ApeKI | GCWGC | 2 cut(s) | 547, 571 |
| AsiGI | ACCGGT | 1 cut(s) | 395 |
| Asp718I | GGTACC | 1 cut(s) | 167 |
| AspLEI | GCGC | 5 cut(s) | 66, 94, 432, 497, 535 |
| AspS9I | GGNCC | 4 cut(s) | 154, 256, 490, 614 |
| AsuC2I | CCSGG | 1 cut(s) | 450 |
| AsuHPI | GGTGA | 2 cut(s) | 385, 596 |
| AvaI | CYCGRG | 1 cut(s) | 576 |
| AvaII | GGWCC | 3 cut(s) | 154, 490, 614 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 159, 192 |
| BanI | GGYRCC | 2 cut(s) | 167, 704 |
| BanII | GRGCYC | 1 cut(s) | 231 |
| BbrPI | CACGTG | 1 cut(s) | 711 |
| BbsI | GAAGAC | 1 cut(s) | 380 |
| Bbv12I | GWGCWC | 1 cut(s) | 231 |
| BbvI | GCAGC | 2 cut(s) | 534, 558 |
| BccI | CCATC | 3 cut(s) | 107, 158, 212 |
| BceAI | ACGGC | 4 cut(s) | 35, 41, 546, 594 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 458, 492 |
| BcnI | CCSGG | 1 cut(s) | 450 |
| BfaI | CTAG | 4 cut(s) | 21, 29, 77, 98 |
| BfoI | RGCGCY | 1 cut(s) | 95 |
| BisI | GCNGC | 7 cut(s) | 95, 334, 466, 548, 572, 607, 699 |
| BlsI | GCNGC | 7 cut(s) | 96, 335, 467, 549, 573, 608, 700 |
| Bme1390I | CCNGG | 1 cut(s) | 450 |
| Bme18I | GGWCC | 3 cut(s) | 154, 490, 614 |
| BmeT110I | CYCGRG | 1 cut(s) | 576 |
| BmgT120I | GGNCC | 4 cut(s) | 154, 256, 490, 614 |
| BmiI | GGNNCC | 4 cut(s) | 155, 169, 616, 706 |
| BmrFI | CCNGG | 1 cut(s) | 450 |
| BoxI | GACNNNNGTC | 1 cut(s) | 384 |
| BpiI | GAAGAC | 1 cut(s) | 380 |
| BpuMI | CCSGG | 1 cut(s) | 450 |
| BsaAI | YACGTR | 1 cut(s) | 711 |
| BsaJI | CCNNGG | 4 cut(s) | 171, 263, 577, 610 |
| BsaWI | WCCGGW | 1 cut(s) | 395 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 549, 579 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 205, 269, 581 |
| Bse118I | RCCGGY | 2 cut(s) | 395, 701 |
| Bse1I | ACTGG | 1 cut(s) | 294 |
| BseDI | CCNNGG | 4 cut(s) | 171, 263, 577, 610 |
| BseGI | GGATG | 1 cut(s) | 253 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 205, 269, 581 |
| BseNI | ACTGG | 1 cut(s) | 294 |
| BseRI | GAGGAG | 4 cut(s) | 83, 594, 602, 655 |
| BseXI | GCAGC | 2 cut(s) | 534, 558 |
| Bsh1285I | CGRYCG | 1 cut(s) | 263 |
| BshFI | GGCC | 1 cut(s) | 258 |
| BshNI | GGYRCC | 2 cut(s) | 167, 704 |
| BshTI | ACCGGT | 1 cut(s) | 395 |
| BsiEI | CGRYCG | 1 cut(s) | 263 |
| BsiHKAI | GWGCWC | 1 cut(s) | 231 |
| BsiHKCI | CYCGRG | 1 cut(s) | 576 |
| BsiSI | CCGG | 3 cut(s) | 396, 449, 702 |
| BsiWI | CGTACG | 1 cut(s) | 238 |
| BslFI | GGGAC | 2 cut(s) | 181, 600 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 205, 269, 581 |
| BsmFI | GGGAC | 2 cut(s) | 181, 600 |
| BsmI | GAATGC | 1 cut(s) | 600 |
| BsnI | GGCC | 1 cut(s) | 258 |
| BsoBI | CYCGRG | 1 cut(s) | 576 |
| Bsp1286I | GDGCHC | 1 cut(s) | 231 |
| Bsp143I | GATC | 2 cut(s) | 415, 537 |
| BspANI | GGCC | 1 cut(s) | 258 |
| BspHI | TCATGA | 1 cut(s) | 283 |
| BspLI | GGNNCC | 4 cut(s) | 155, 169, 616, 706 |
| BspPI | GGATC | 1 cut(s) | 545 |
| BspT107I | GGYRCC | 2 cut(s) | 167, 704 |
| BsrFI | RCCGGY | 2 cut(s) | 395, 701 |
| BsrI | ACTGG | 1 cut(s) | 294 |
| BssAI | RCCGGY | 2 cut(s) | 395, 701 |
| BssECI | CCNNGG | 4 cut(s) | 171, 263, 577, 610 |
| BssMI | GATC | 2 cut(s) | 415, 537 |
| BssT1I | CCWWGG | 1 cut(s) | 171 |
| Bst4CI | ACNGT | 3 cut(s) | 264, 423, 658 |
| Bst6I | CTCTTC | 3 cut(s) | 129, 174, 321 |
| BstBAI | YACGTR | 1 cut(s) | 711 |
| BstC8I | GCNNGC | 5 cut(s) | 187, 231, 235, 463, 552 |
| BstDSI | CCRYGG | 2 cut(s) | 263, 610 |
| BstF5I | GGATG | 1 cut(s) | 253 |
| BstH2I | RGCGCY | 1 cut(s) | 95 |
| BstHHI | GCGC | 5 cut(s) | 66, 94, 432, 497, 535 |
| BstKTI | GATC | 2 cut(s) | 418, 540 |
| BstMBI | GATC | 2 cut(s) | 415, 537 |
| BstMCI | CGRYCG | 1 cut(s) | 263 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 539, 688, 704 |
| BstNSI | RCATGY | 1 cut(s) | 206 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 384 |
| BstSCI | CCNGG | 1 cut(s) | 448 |
| BstV1I | GCAGC | 2 cut(s) | 534, 558 |
| BstV2I | GAAGAC | 1 cut(s) | 380 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 487 |
| BsuRI | GGCC | 1 cut(s) | 258 |
| BtgI | CCRYGG | 2 cut(s) | 263, 610 |
| BtgZI | GCGATG | 2 cut(s) | 126, 237 |
| BtsCI | GGATG | 1 cut(s) | 253 |
| Cac8I | GCNNGC | 5 cut(s) | 187, 231, 235, 463, 552 |
| CciI | TCATGA | 1 cut(s) | 283 |
| CfoI | GCGC | 5 cut(s) | 66, 94, 432, 497, 535 |
| Cfr10I | RCCGGY | 2 cut(s) | 395, 701 |
| Cfr13I | GGNCC | 4 cut(s) | 154, 256, 490, 614 |
| CseI | GACGC | 3 cut(s) | 325, 436, 479 |
| Csp6I | GTAC | 3 cut(s) | 168, 239, 273 |
| CspAI | ACCGGT | 1 cut(s) | 395 |
| CviAII | CATG | 3 cut(s) | 150, 203, 284 |
| CviQI | GTAC | 3 cut(s) | 168, 239, 273 |
| DpnI | GATC | 2 cut(s) | 417, 539 |
| DpnII | GATC | 2 cut(s) | 415, 537 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 50 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 50 |
| Eam1104I | CTCTTC | 3 cut(s) | 129, 174, 321 |
| EarI | CTCTTC | 3 cut(s) | 129, 174, 321 |
| EciI | GGCGGA | 3 cut(s) | 144, 376, 435 |
| Ecl136II | GAGCTC | 1 cut(s) | 229 |
| Eco130I | CCWWGG | 1 cut(s) | 171 |
| Eco24I | GRGCYC | 1 cut(s) | 231 |
| Eco47I | GGWCC | 3 cut(s) | 154, 490, 614 |
| Eco53kI | GAGCTC | 1 cut(s) | 229 |
| Eco72I | CACGTG | 1 cut(s) | 711 |
| Eco88I | CYCGRG | 1 cut(s) | 576 |
| EcoICRI | GAGCTC | 1 cut(s) | 229 |
| EcoO109I | RGGNCCY | 1 cut(s) | 154 |
| EcoT14I | CCWWGG | 1 cut(s) | 171 |
| EcoT38I | GRGCYC | 1 cut(s) | 231 |
| ErhI | CCWWGG | 1 cut(s) | 171 |
| FaeI | CATG | 3 cut(s) | 153, 206, 287 |
| FaiI | YATR | 6 cut(s) | 151, 204, 285, 309, 314, 488 |
| FaqI | GGGAC | 2 cut(s) | 181, 600 |
| FatI | CATG | 3 cut(s) | 149, 202, 283 |
| FauI | CCCGC | 4 cut(s) | 178, 535, 543, 547 |
| Fnu4HI | GCNGC | 7 cut(s) | 95, 334, 466, 548, 572, 607, 699 |
| FokI | GGATG | 1 cut(s) | 260 |
| FriOI | GRGCYC | 1 cut(s) | 231 |
| Fsp4HI | GCNGC | 7 cut(s) | 95, 334, 466, 548, 572, 607, 699 |
| FspBI | CTAG | 4 cut(s) | 21, 29, 77, 98 |
| GlaI | GCGC | 5 cut(s) | 65, 93, 431, 496, 534 |
| GluI | GCNGC | 7 cut(s) | 95, 334, 466, 548, 572, 607, 699 |
| HaeII | RGCGCY | 1 cut(s) | 95 |
| HaeIII | GGCC | 1 cut(s) | 258 |
| HapII | CCGG | 3 cut(s) | 396, 449, 702 |
| HgaI | GACGC | 3 cut(s) | 325, 436, 479 |
| HhaI | GCGC | 5 cut(s) | 66, 94, 432, 497, 535 |
| Hin1II | CATG | 3 cut(s) | 153, 206, 287 |
| Hin6I | GCGC | 5 cut(s) | 64, 92, 430, 495, 533 |
| HinP1I | GCGC | 5 cut(s) | 64, 92, 430, 495, 533 |
| HincII | GTYRAC | 1 cut(s) | 426 |
| HindII | GTYRAC | 1 cut(s) | 426 |
| HindIII | AAGCTT | 1 cut(s) | 500 |
| HinfI | GANTC | 2 cut(s) | 143, 440 |
| HpaII | CCGG | 3 cut(s) | 396, 449, 702 |
| HphI | GGTGA | 2 cut(s) | 385, 596 |
| Hpy166II | GTNNAC | 1 cut(s) | 426 |
| Hpy188I | TCNGA | 1 cut(s) | 523 |
| Hpy188III | TCNNGA | 3 cut(s) | 161, 245, 284 |
| Hpy8I | GTNNAC | 1 cut(s) | 426 |
| Hpy99I | CGWCG | 4 cut(s) | 56, 62, 246, 473 |
| HpyAV | CCTTC | 3 cut(s) | 167, 247, 392 |
| HpyCH4III | ACNGT | 3 cut(s) | 264, 423, 658 |
| HpyCH4IV | ACGT | 2 cut(s) | 241, 710 |
| HpyCH4V | TGCA | 1 cut(s) | 682 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 539, 688, 704 |
| HpySE526I | ACGT | 2 cut(s) | 241, 710 |
| Hsp92II | CATG | 3 cut(s) | 153, 206, 287 |
| HspAI | GCGC | 5 cut(s) | 64, 92, 430, 495, 533 |
| KpnI | GGTACC | 1 cut(s) | 171 |
| Kzo9I | GATC | 2 cut(s) | 415, 537 |
| LmnI | GCTCC | 1 cut(s) | 557 |
| LpnPI | CCDG | 6 cut(s) | 93, 219, 275, 409, 462, 616 |
| Lsp1109I | GCAGC | 2 cut(s) | 534, 558 |
| MaeI | CTAG | 4 cut(s) | 21, 29, 77, 98 |
| MaeII | ACGT | 2 cut(s) | 241, 710 |
| MaeIII | GTNAC | 2 cut(s) | 452, 652 |
| MalI | GATC | 2 cut(s) | 417, 539 |
| MboI | GATC | 2 cut(s) | 415, 537 |
| MboII | GAAGA | 4 cut(s) | 146, 191, 338, 380 |
| MhlI | GDGCHC | 1 cut(s) | 231 |
| MluCI | AATT | 1 cut(s) | 508 |
| MluI | ACGCGT | 1 cut(s) | 317 |
| MlyI | GAGTC | 1 cut(s) | 152 |
| MmeI | TCCRAC | 1 cut(s) | 648 |
| MspI | CCGG | 3 cut(s) | 396, 449, 702 |
| MspR9I | CCNGG | 1 cut(s) | 450 |
| Mva1269I | GAATGC | 1 cut(s) | 600 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 539, 688, 704 |
| NciI | CCSGG | 1 cut(s) | 450 |
| NdeII | GATC | 2 cut(s) | 415, 537 |
| NlaIII | CATG | 3 cut(s) | 153, 206, 287 |
| NlaIV | GGNNCC | 4 cut(s) | 155, 169, 616, 706 |
| NspI | RCATGY | 1 cut(s) | 206 |
| PagI | TCATGA | 1 cut(s) | 283 |
| PciI | ACATGT | 1 cut(s) | 202 |
| PctI | GAATGC | 1 cut(s) | 600 |
| PfeI | GAWTC | 1 cut(s) | 440 |
| Pfl23II | CGTACG | 1 cut(s) | 238 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 205 |
| PinAI | ACCGGT | 1 cut(s) | 395 |
| PkrI | GCNGC | 7 cut(s) | 96, 335, 467, 549, 573, 608, 700 |
| PleI | GAGTC | 1 cut(s) | 151 |
| PmaCI | CACGTG | 1 cut(s) | 711 |
| PmlI | CACGTG | 1 cut(s) | 711 |
| PpsI | GAGTC | 1 cut(s) | 151 |
| Ppu21I | YACGTR | 1 cut(s) | 711 |
| PpuMI | RGGWCCY | 1 cut(s) | 154 |
| PscI | ACATGT | 1 cut(s) | 202 |
| PshAI | GACNNNNGTC | 1 cut(s) | 384 |
| PsiI | TTATAA | 1 cut(s) | 309 |
| Psp124BI | GAGCTC | 1 cut(s) | 231 |
| Psp5II | RGGWCCY | 1 cut(s) | 154 |
| PspCI | CACGTG | 1 cut(s) | 711 |
| PspLI | CGTACG | 1 cut(s) | 238 |
| PspN4I | GGNNCC | 4 cut(s) | 155, 169, 616, 706 |
| PspPI | GGNCC | 4 cut(s) | 154, 256, 490, 614 |
| PspPPI | RGGWCCY | 1 cut(s) | 154 |
| RsaI | GTAC | 3 cut(s) | 169, 240, 274 |
| RsaNI | GTAC | 3 cut(s) | 168, 239, 273 |
| SacI | GAGCTC | 1 cut(s) | 231 |
| SatI | GCNGC | 7 cut(s) | 95, 334, 466, 548, 572, 607, 699 |
| Sau3AI | GATC | 2 cut(s) | 415, 537 |
| Sau96I | GGNCC | 4 cut(s) | 154, 256, 490, 614 |
| SchI | GAGTC | 1 cut(s) | 152 |
| ScrFI | CCNGG | 1 cut(s) | 450 |
| SduI | GDGCHC | 1 cut(s) | 231 |
| SgrAI | CRCCGGYG | 1 cut(s) | 701 |
| SinI | GGWCC | 3 cut(s) | 154, 490, 614 |
| Sse9I | AATT | 1 cut(s) | 508 |
| SspMI | CTAG | 4 cut(s) | 21, 29, 77, 98 |
| SstI | GAGCTC | 1 cut(s) | 231 |
| StyD4I | CCNGG | 1 cut(s) | 448 |
| StyI | CCWWGG | 1 cut(s) | 171 |
| TaaI | ACNGT | 3 cut(s) | 264, 423, 658 |
| TaiI | ACGT | 2 cut(s) | 244, 713 |
| TaqI | TCGA | 2 cut(s) | 414, 638 |
| TasI | AATT | 1 cut(s) | 508 |
| TauI | GCSGC | 5 cut(s) | 97, 336, 468, 609, 701 |
| TfiI | GAWTC | 1 cut(s) | 440 |
| TseI | GCWGC | 2 cut(s) | 547, 571 |
| TspDTI | ATGAA | 2 cut(s) | 272, 300 |
| TspGWI | ACGGA | 1 cut(s) | 540 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 205 |
| VpaK11BI | GGWCC | 3 cut(s) | 154, 490, 614 |
| XceI | RCATGY | 1 cut(s) | 206 |
| XspI | CTAG | 4 cut(s) | 21, 29, 77, 98 |