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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1392 |
| Acc36I | ACCTGC | 1 cut(s) | 198 |
| AccB1I | GGYRCC | 2 cut(s) | 157, 1347 |
| AccI | GTMKAC | 1 cut(s) | 1053 |
| AccIII | TCCGGA | 1 cut(s) | 246 |
| AclWI | GGATC | 4 cut(s) | 86, 99, 1214, 1284 |
| AcoI | YGGCCR | 1 cut(s) | 636 |
| AcsI | RAATTY | 3 cut(s) | 60, 267, 1091 |
| AcuI | CTGAAG | 2 cut(s) | 492, 605 |
| AcyI | GRCGYC | 1 cut(s) | 1348 |
| AfaI | GTAC | 1 cut(s) | 911 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 169, 196, 246, 622 |
| AflII | CTTAAG | 1 cut(s) | 1370 |
| AflIII | ACRYGT | 1 cut(s) | 429 |
| AgeI | ACCGGT | 1 cut(s) | 231 |
| AgsI | TTSAA | 2 cut(s) | 274, 1039 |
| AjiI | CACGTC | 5 cut(s) | 357, 406, 432, 481, 533 |
| AjnI | CCWGG | 2 cut(s) | 414, 489 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 951, 983 |
| Alw21I | GWGCWC | 3 cut(s) | 30, 1227, 1461 |
| Alw26I | GTCTC | 2 cut(s) | 955, 1159 |
| AlwI | GGATC | 4 cut(s) | 86, 99, 1214, 1284 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 389, 464 |
| Ama87I | CYCGRG | 1 cut(s) | 782 |
| Aor13HI | TCCGGA | 1 cut(s) | 246 |
| AoxI | GGCC | 4 cut(s) | 171, 520, 589, 636 |
| ApaI | GGGCCC | 1 cut(s) | 524 |
| ApeKI | GCWGC | 1 cut(s) | 842 |
| ApoI | RAATTY | 3 cut(s) | 60, 267, 1091 |
| AsiGI | ACCGGT | 1 cut(s) | 231 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1068 |
| AspLEI | GCGC | 2 cut(s) | 1334, 1350 |
| AspS9I | GGNCC | 8 cut(s) | 249, 393, 419, 468, 494, 520, 521, 1394 |
| AsuHPI | GGTGA | 4 cut(s) | 229, 589, 661, 1467 |
| AvaI | CYCGRG | 1 cut(s) | 782 |
| AvaII | GGWCC | 6 cut(s) | 249, 393, 419, 468, 494, 1394 |
| BaeGI | GKGCMC | 1 cut(s) | 524 |
| BamHI | GGATCC | 1 cut(s) | 91 |
| BanI | GGYRCC | 2 cut(s) | 157, 1347 |
| BanII | GRGCYC | 4 cut(s) | 30, 524, 1227, 1461 |
| BauI | CACGAG | 1 cut(s) | 1460 |
| BbsI | GAAGAC | 2 cut(s) | 936, 1139 |
| Bbv12I | GWGCWC | 3 cut(s) | 30, 1227, 1461 |
| BbvI | GCAGC | 1 cut(s) | 854 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1178, 1212 |
| BciT130I | CCWGG | 2 cut(s) | 416, 491 |
| BciVI | GTATCC | 2 cut(s) | 30, 1369 |
| BcoDI | GTCTC | 2 cut(s) | 955, 1159 |
| BfaI | CTAG | 3 cut(s) | 789, 1001, 1546 |
| BfoI | RGCGCY | 1 cut(s) | 1351 |
| BfrI | CTTAAG | 1 cut(s) | 1370 |
| BfuAI | ACCTGC | 1 cut(s) | 198 |
| BfuI | GTATCC | 2 cut(s) | 30, 1369 |
| BglII | AGATCT | 1 cut(s) | 1542 |
| BisI | GCNGC | 3 cut(s) | 153, 156, 843 |
| BlsI | GCNGC | 3 cut(s) | 154, 157, 844 |
| Bme1390I | CCNGG | 2 cut(s) | 416, 491 |
| Bme18I | GGWCC | 6 cut(s) | 249, 393, 419, 468, 494, 1394 |
| BmeT110I | CYCGRG | 1 cut(s) | 782 |
| BmgBI | CACGTC | 5 cut(s) | 357, 406, 432, 481, 533 |
| BmgT120I | GGNCC | 8 cut(s) | 249, 393, 419, 468, 494, 520, 521, 1394 |
| BmrFI | CCNGG | 2 cut(s) | 416, 491 |
| BmsI | GCATC | 3 cut(s) | 395, 470, 757 |
| BpiI | GAAGAC | 2 cut(s) | 936, 1139 |
| BpmI | CTGGAG | 1 cut(s) | 890 |
| BpuEI | CTTGAG | 2 cut(s) | 737, 804 |
| Bsa29I | ATCGAT | 1 cut(s) | 1188 |
| BsaBI | GATNNNNATC | 1 cut(s) | 90 |
| BsaHI | GRCGYC | 1 cut(s) | 1348 |
| BsaI | GGTCTC | 1 cut(s) | 955 |
| BsaJI | CCNNGG | 5 cut(s) | 414, 415, 489, 490, 1293 |
| BsaWI | WCCGGW | 3 cut(s) | 231, 246, 672 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 169, 196, 246, 622 |
| Bse118I | RCCGGY | 3 cut(s) | 148, 231, 574 |
| Bse1I | ACTGG | 3 cut(s) | 592, 712, 907 |
| Bse3DI | GCAATG | 1 cut(s) | 979 |
| Bse8I | GATNNNNATC | 1 cut(s) | 90 |
| BseAI | TCCGGA | 1 cut(s) | 246 |
| BseBI | CCWGG | 2 cut(s) | 416, 491 |
| BseCI | ATCGAT | 1 cut(s) | 1188 |
| BseDI | CCNNGG | 5 cut(s) | 414, 415, 489, 490, 1293 |
| BseJI | GATNNNNATC | 1 cut(s) | 90 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 169, 196, 246, 622 |
| BseMI | GCAATG | 1 cut(s) | 979 |
| BseMII | CTCAG | 1 cut(s) | 553 |
| BseNI | ACTGG | 3 cut(s) | 592, 712, 907 |
| BseRI | GAGGAG | 1 cut(s) | 70 |
| BseSI | GKGCMC | 1 cut(s) | 524 |
| BseXI | GCAGC | 1 cut(s) | 854 |
| Bsh1285I | CGRYCG | 1 cut(s) | 585 |
| BshFI | GGCC | 4 cut(s) | 173, 522, 591, 638 |
| BshNI | GGYRCC | 2 cut(s) | 157, 1347 |
| BshTI | ACCGGT | 1 cut(s) | 231 |
| BshVI | ATCGAT | 1 cut(s) | 1188 |
| BsiEI | CGRYCG | 1 cut(s) | 585 |
| BsiHKAI | GWGCWC | 3 cut(s) | 30, 1227, 1461 |
| BsiHKCI | CYCGRG | 1 cut(s) | 782 |
| BsiSI | CCGG | 7 cut(s) | 149, 174, 232, 247, 575, 635, 673 |
| BslFI | GGGAC | 6 cut(s) | 81, 406, 432, 481, 507, 962 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 169, 196, 246, 622 |
| BsmAI | GTCTC | 2 cut(s) | 955, 1159 |
| BsmBI | CGTCTC | 1 cut(s) | 1159 |
| BsmFI | GGGAC | 6 cut(s) | 81, 406, 432, 481, 507, 962 |
| BsnI | GGCC | 4 cut(s) | 173, 522, 591, 638 |
| Bso31I | GGTCTC | 1 cut(s) | 955 |
| BsoBI | CYCGRG | 1 cut(s) | 782 |
| Bsp120I | GGGCCC | 1 cut(s) | 520 |
| Bsp1286I | GDGCHC | 4 cut(s) | 30, 524, 1227, 1461 |
| Bsp13I | TCCGGA | 1 cut(s) | 246 |
| Bsp143I | GATC | 7 cut(s) | 91, 582, 1107, 1206, 1289, 1530, 1542 |
| BspANI | GGCC | 4 cut(s) | 173, 522, 591, 638 |
| BspCNI | CTCAG | 1 cut(s) | 552 |
| BspDI | ATCGAT | 1 cut(s) | 1188 |
| BspEI | TCCGGA | 1 cut(s) | 246 |
| BspHI | TCATGA | 1 cut(s) | 1219 |
| BspMI | ACCTGC | 1 cut(s) | 198 |
| BspPI | GGATC | 4 cut(s) | 86, 99, 1214, 1284 |
| BspT107I | GGYRCC | 2 cut(s) | 157, 1347 |
| BspTI | CTTAAG | 1 cut(s) | 1370 |
| BspTNI | GGTCTC | 1 cut(s) | 955 |
| BsrDI | GCAATG | 1 cut(s) | 979 |
| BsrFI | RCCGGY | 3 cut(s) | 148, 231, 574 |
| BsrI | ACTGG | 3 cut(s) | 592, 712, 907 |
| BssAI | RCCGGY | 3 cut(s) | 148, 231, 574 |
| BssECI | CCNNGG | 5 cut(s) | 414, 415, 489, 490, 1293 |
| BssMI | GATC | 7 cut(s) | 91, 582, 1107, 1206, 1289, 1530, 1542 |
| BssNI | GRCGYC | 1 cut(s) | 1348 |
| BssSI | CACGAG | 1 cut(s) | 1460 |
| Bst2BI | CACGAG | 1 cut(s) | 1460 |
| Bst2UI | CCWGG | 2 cut(s) | 416, 491 |
| Bst4CI | ACNGT | 4 cut(s) | 369, 560, 567, 808 |
| Bst6I | CTCTTC | 1 cut(s) | 1447 |
| BstACI | GRCGYC | 1 cut(s) | 1348 |
| BstAFI | CTTAAG | 1 cut(s) | 1370 |
| BstC8I | GCNNGC | 3 cut(s) | 589, 746, 816 |
| BstDEI | CTNAG | 3 cut(s) | 539, 1173, 1338 |
| BstDSI | CCRYGG | 1 cut(s) | 1293 |
| BstEII | GGTNACC | 1 cut(s) | 1421 |
| BstH2I | RGCGCY | 1 cut(s) | 1351 |
| BstHHI | GCGC | 2 cut(s) | 1334, 1350 |
| BstKTI | GATC | 7 cut(s) | 94, 585, 1110, 1209, 1292, 1533, 1545 |
| BstMAI | GTCTC | 2 cut(s) | 955, 1159 |
| BstMBI | GATC | 7 cut(s) | 91, 582, 1107, 1206, 1289, 1530, 1542 |
| BstMCI | CGRYCG | 1 cut(s) | 585 |
| BstNI | CCWGG | 2 cut(s) | 416, 491 |
| BstPI | GGTNACC | 1 cut(s) | 1421 |
| BstSCI | CCNGG | 2 cut(s) | 414, 489 |
| BstSLI | GKGCMC | 1 cut(s) | 524 |
| BstV1I | GCAGC | 1 cut(s) | 854 |
| BstV2I | GAAGAC | 2 cut(s) | 936, 1139 |
| BstX2I | RGATCY | 3 cut(s) | 91, 1289, 1542 |
| BstYI | RGATCY | 3 cut(s) | 91, 1289, 1542 |
| Bsu15I | ATCGAT | 1 cut(s) | 1188 |
| BsuI | GTATCC | 2 cut(s) | 30, 1369 |
| BsuRI | GGCC | 4 cut(s) | 173, 522, 591, 638 |
| BsuTUI | ATCGAT | 1 cut(s) | 1188 |
| BtgI | CCRYGG | 1 cut(s) | 1293 |
| BtgZI | GCGATG | 1 cut(s) | 1133 |
| BtrI | CACGTC | 5 cut(s) | 357, 406, 432, 481, 533 |
| BtsI | GCAGTG | 1 cut(s) | 524 |
| BtsIMutI | CAGTG | 4 cut(s) | 333, 423, 524, 1442 |
| BveI | ACCTGC | 1 cut(s) | 198 |
| Cac8I | GCNNGC | 3 cut(s) | 589, 746, 816 |
| CaiI | CAGNNNCTG | 2 cut(s) | 389, 464 |
| CciI | TCATGA | 1 cut(s) | 1219 |
| CfoI | GCGC | 2 cut(s) | 1334, 1350 |
| Cfr10I | RCCGGY | 3 cut(s) | 148, 231, 574 |
| Cfr13I | GGNCC | 8 cut(s) | 249, 393, 419, 468, 494, 520, 521, 1394 |
| ClaI | ATCGAT | 1 cut(s) | 1188 |
| CseI | GACGC | 1 cut(s) | 347 |
| Csp6I | GTAC | 1 cut(s) | 910 |
| CspAI | ACCGGT | 1 cut(s) | 231 |
| CviAII | CATG | 2 cut(s) | 1220, 1256 |
| CviQI | GTAC | 1 cut(s) | 910 |
| DdeI | CTNAG | 3 cut(s) | 539, 1173, 1338 |
| DinI | GGCGCC | 1 cut(s) | 1349 |
| DpnI | GATC | 7 cut(s) | 93, 584, 1109, 1208, 1291, 1532, 1544 |
| DpnII | GATC | 7 cut(s) | 91, 582, 1107, 1206, 1289, 1530, 1542 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1392 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1392 |
| EaeI | YGGCCR | 1 cut(s) | 636 |
| Eam1104I | CTCTTC | 1 cut(s) | 1447 |
| EarI | CTCTTC | 1 cut(s) | 1447 |
| EciI | GGCGGA | 3 cut(s) | 38, 439, 731 |
| Ecl136II | GAGCTC | 3 cut(s) | 28, 1225, 1459 |
| Eco24I | GRGCYC | 4 cut(s) | 30, 524, 1227, 1461 |
| Eco31I | GGTCTC | 1 cut(s) | 955 |
| Eco32I | GATATC | 1 cut(s) | 739 |
| Eco47I | GGWCC | 6 cut(s) | 249, 393, 419, 468, 494, 1394 |
| Eco53kI | GAGCTC | 3 cut(s) | 28, 1225, 1459 |
| Eco57I | CTGAAG | 2 cut(s) | 492, 605 |
| Eco88I | CYCGRG | 1 cut(s) | 782 |
| Eco91I | GGTNACC | 1 cut(s) | 1421 |
| EcoICRI | GAGCTC | 3 cut(s) | 28, 1225, 1459 |
| EcoO109I | RGGNCCY | 5 cut(s) | 393, 419, 468, 494, 520 |
| EcoO65I | GGTNACC | 1 cut(s) | 1421 |
| EcoRI | GAATTC | 1 cut(s) | 60 |
| EcoRII | CCWGG | 2 cut(s) | 414, 489 |
| EcoRV | GATATC | 1 cut(s) | 739 |
| EcoT38I | GRGCYC | 4 cut(s) | 30, 524, 1227, 1461 |
| EgeI | GGCGCC | 1 cut(s) | 1349 |
| EheI | GGCGCC | 1 cut(s) | 1349 |
| Esp3I | CGTCTC | 1 cut(s) | 1159 |
| FaeI | CATG | 2 cut(s) | 1223, 1259 |
| FaqI | GGGAC | 6 cut(s) | 81, 406, 432, 481, 507, 962 |
| FatI | CATG | 2 cut(s) | 1219, 1255 |
| FauI | CCCGC | 1 cut(s) | 11 |
| FauNDI | CATATG | 2 cut(s) | 549, 891 |
| FblI | GTMKAC | 1 cut(s) | 1053 |
| Fnu4HI | GCNGC | 3 cut(s) | 153, 156, 843 |
| FriOI | GRGCYC | 4 cut(s) | 30, 524, 1227, 1461 |
| Fsp4HI | GCNGC | 3 cut(s) | 153, 156, 843 |
| FspBI | CTAG | 3 cut(s) | 789, 1001, 1546 |
| GlaI | GCGC | 2 cut(s) | 1333, 1349 |
| GluI | GCNGC | 3 cut(s) | 153, 156, 843 |
| GsuI | CTGGAG | 1 cut(s) | 890 |
| HaeII | RGCGCY | 1 cut(s) | 1351 |
| HaeIII | GGCC | 4 cut(s) | 173, 522, 591, 638 |
| HapII | CCGG | 7 cut(s) | 149, 174, 232, 247, 575, 635, 673 |
| HgaI | GACGC | 1 cut(s) | 347 |
| HhaI | GCGC | 2 cut(s) | 1334, 1350 |
| Hin1I | GRCGYC | 1 cut(s) | 1348 |
| Hin1II | CATG | 2 cut(s) | 1223, 1259 |
| Hin6I | GCGC | 2 cut(s) | 1332, 1348 |
| HinP1I | GCGC | 2 cut(s) | 1332, 1348 |
| HincII | GTYRAC | 3 cut(s) | 556, 563, 570 |
| HindII | GTYRAC | 3 cut(s) | 556, 563, 570 |
| HindIII | AAGCTT | 1 cut(s) | 822 |
| HinfI | GANTC | 6 cut(s) | 765, 778, 883, 899, 1135, 1316 |
| HpaII | CCGG | 7 cut(s) | 149, 174, 232, 247, 575, 635, 673 |
| HphI | GGTGA | 4 cut(s) | 229, 589, 661, 1467 |
| Hpy166II | GTNNAC | 5 cut(s) | 237, 556, 563, 570, 1054 |
| Hpy188I | TCNGA | 5 cut(s) | 613, 663, 715, 1063, 1315 |
| Hpy188III | TCNNGA | 9 cut(s) | 197, 247, 782, 995, 1220, 1287, 1383, 1462, 1534 |
| Hpy8I | GTNNAC | 5 cut(s) | 237, 556, 563, 570, 1054 |
| HpyAV | CCTTC | 5 cut(s) | 629, 933, 1156, 1338, 1412 |
| HpyCH4III | ACNGT | 4 cut(s) | 369, 560, 567, 808 |
| HpyCH4IV | ACGT | 7 cut(s) | 356, 405, 431, 480, 532, 798, 1387 |
| HpyCH4V | TGCA | 1 cut(s) | 1199 |
| HpyF3I | CTNAG | 3 cut(s) | 539, 1173, 1338 |
| HpySE526I | ACGT | 7 cut(s) | 356, 405, 431, 480, 532, 798, 1387 |
| Hsp92I | GRCGYC | 1 cut(s) | 1348 |
| Hsp92II | CATG | 2 cut(s) | 1223, 1259 |
| HspAI | GCGC | 2 cut(s) | 1332, 1348 |
| KasI | GGCGCC | 1 cut(s) | 1347 |
| KflI | GGGWCCC | 4 cut(s) | 393, 419, 468, 494 |
| Kpn2I | TCCGGA | 1 cut(s) | 246 |
| Kzo9I | GATC | 7 cut(s) | 91, 582, 1107, 1206, 1289, 1530, 1542 |
| LmnI | GCTCC | 5 cut(s) | 25, 208, 697, 909, 1232 |
| Lsp1109I | GCAGC | 1 cut(s) | 854 |
| LweI | GCATC | 3 cut(s) | 395, 470, 757 |
| MaeI | CTAG | 3 cut(s) | 789, 1001, 1546 |
| MaeII | ACGT | 7 cut(s) | 356, 405, 431, 480, 532, 798, 1387 |
| MaeIII | GTNAC | 5 cut(s) | 376, 577, 668, 1421, 1473 |
| MalI | GATC | 7 cut(s) | 93, 584, 1109, 1208, 1291, 1532, 1544 |
| MboI | GATC | 7 cut(s) | 91, 582, 1107, 1206, 1289, 1530, 1542 |
| MflI | RGATCY | 3 cut(s) | 91, 1289, 1542 |
| MhlI | GDGCHC | 4 cut(s) | 30, 524, 1227, 1461 |
| MluCI | AATT | 7 cut(s) | 60, 256, 267, 680, 1091, 1282, 1321 |
| Mly113I | GGCGCC | 1 cut(s) | 1348 |
| MlyI | GAGTC | 2 cut(s) | 877, 1129 |
| MmeI | TCCRAC | 1 cut(s) | 636 |
| MnlI | CCTC | 8 cut(s) | 48, 76, 204, 262, 307, 844, 1031, 1196 |
| MroI | TCCGGA | 1 cut(s) | 246 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1068 |
| MseI | TTAA | 4 cut(s) | 111, 1077, 1085, 1371 |
| MslI | CAYNNNNRTG | 3 cut(s) | 878, 894, 1268 |
| MspA1I | CMGCKG | 1 cut(s) | 18 |
| MspCI | CTTAAG | 1 cut(s) | 1370 |
| MspI | CCGG | 7 cut(s) | 149, 174, 232, 247, 575, 635, 673 |
| MspR9I | CCNGG | 2 cut(s) | 416, 491 |
| MvaI | CCWGG | 2 cut(s) | 416, 491 |
| NarI | GGCGCC | 1 cut(s) | 1348 |
| NdeI | CATATG | 2 cut(s) | 549, 891 |
| NdeII | GATC | 7 cut(s) | 91, 582, 1107, 1206, 1289, 1530, 1542 |
| NlaIII | CATG | 2 cut(s) | 1223, 1259 |
| NmuCI | GTSAC | 3 cut(s) | 376, 577, 1473 |
| PaeR7I | CTCGAG | 1 cut(s) | 782 |
| PagI | TCATGA | 1 cut(s) | 1219 |
| PasI | CCCWGGG | 2 cut(s) | 415, 490 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 578 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1068 |
| PfeI | GAWTC | 4 cut(s) | 765, 778, 899, 1316 |
| PinAI | ACCGGT | 1 cut(s) | 231 |
| PkrI | GCNGC | 3 cut(s) | 154, 157, 844 |
| Ple19I | CGATCG | 1 cut(s) | 585 |
| PleI | GAGTC | 2 cut(s) | 877, 1129 |
| PluTI | GGCGCC | 1 cut(s) | 1351 |
| PpsI | GAGTC | 2 cut(s) | 877, 1129 |
| PpuMI | RGGWCCY | 4 cut(s) | 393, 419, 468, 494 |
| Psp124BI | GAGCTC | 3 cut(s) | 30, 1227, 1461 |
| Psp5II | RGGWCCY | 4 cut(s) | 393, 419, 468, 494 |
| Psp6I | CCWGG | 2 cut(s) | 414, 489 |
| PspEI | GGTNACC | 1 cut(s) | 1421 |
| PspGI | CCWGG | 2 cut(s) | 414, 489 |
| PspOMI | GGGCCC | 1 cut(s) | 520 |
| PspPI | GGNCC | 8 cut(s) | 249, 393, 419, 468, 494, 520, 521, 1394 |
| PspPPI | RGGWCCY | 4 cut(s) | 393, 419, 468, 494 |
| PstNI | CAGNNNCTG | 2 cut(s) | 389, 464 |
| PsuI | RGATCY | 3 cut(s) | 91, 1289, 1542 |
| PvuI | CGATCG | 1 cut(s) | 585 |
| RsaI | GTAC | 1 cut(s) | 911 |
| RsaNI | GTAC | 1 cut(s) | 910 |
| RseI | CAYNNNNRTG | 3 cut(s) | 878, 894, 1268 |
| SacI | GAGCTC | 3 cut(s) | 30, 1227, 1461 |
| SaqAI | TTAA | 4 cut(s) | 111, 1077, 1085, 1371 |
| SatI | GCNGC | 3 cut(s) | 153, 156, 843 |
| Sau3AI | GATC | 7 cut(s) | 91, 582, 1107, 1206, 1289, 1530, 1542 |
| Sau96I | GGNCC | 8 cut(s) | 249, 393, 419, 468, 494, 520, 521, 1394 |
| SchI | GAGTC | 2 cut(s) | 877, 1129 |
| ScrFI | CCNGG | 2 cut(s) | 416, 491 |
| SduI | GDGCHC | 4 cut(s) | 30, 524, 1227, 1461 |
| SfaNI | GCATC | 3 cut(s) | 395, 470, 757 |
| SfoI | GGCGCC | 1 cut(s) | 1349 |
| Sfr274I | CTCGAG | 1 cut(s) | 782 |
| SgrAI | CRCCGGYG | 1 cut(s) | 574 |
| SinI | GGWCC | 6 cut(s) | 249, 393, 419, 468, 494, 1394 |
| SlaI | CTCGAG | 1 cut(s) | 782 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 878, 894, 1268 |
| SmlI | CTYRAG | 4 cut(s) | 752, 782, 819, 1370 |
| SmoI | CTYRAG | 4 cut(s) | 752, 782, 819, 1370 |
| Sse9I | AATT | 7 cut(s) | 60, 256, 267, 680, 1091, 1282, 1321 |
| SspDI | GGCGCC | 1 cut(s) | 1347 |
| SspI | AATATT | 1 cut(s) | 225 |
| SspMI | CTAG | 3 cut(s) | 789, 1001, 1546 |
| SstI | GAGCTC | 3 cut(s) | 30, 1227, 1461 |
| StyD4I | CCNGG | 2 cut(s) | 414, 489 |
| TaaI | ACNGT | 4 cut(s) | 369, 560, 567, 808 |
| TaiI | ACGT | 7 cut(s) | 359, 408, 434, 483, 535, 801, 1390 |
| TaqI | TCGA | 4 cut(s) | 783, 957, 1188, 1286 |
| TasI | AATT | 7 cut(s) | 60, 256, 267, 680, 1091, 1282, 1321 |
| TauI | GCSGC | 2 cut(s) | 155, 158 |
| TfiI | GAWTC | 4 cut(s) | 765, 778, 899, 1316 |
| Tru1I | TTAA | 4 cut(s) | 111, 1077, 1085, 1371 |
| Tru9I | TTAA | 4 cut(s) | 111, 1077, 1085, 1371 |
| TscAI | CASTG | 4 cut(s) | 340, 430, 531, 1449 |
| TseFI | GTSAC | 3 cut(s) | 376, 577, 1473 |
| TseI | GCWGC | 1 cut(s) | 842 |
| Tsp45I | GTSAC | 3 cut(s) | 376, 577, 1473 |
| TspDTI | ATGAA | 5 cut(s) | 159, 935, 939, 1208, 1253 |
| TspGWI | ACGGA | 2 cut(s) | 364, 1282 |
| TspRI | CASTG | 4 cut(s) | 340, 430, 531, 1449 |
| Vha464I | CTTAAG | 1 cut(s) | 1370 |
| VpaK11BI | GGWCC | 6 cut(s) | 249, 393, 419, 468, 494, 1394 |
| XapI | RAATTY | 3 cut(s) | 60, 267, 1091 |
| XhoI | CTCGAG | 1 cut(s) | 782 |
| XmiI | GTMKAC | 1 cut(s) | 1053 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1068 |
| XspI | CTAG | 3 cut(s) | 789, 1001, 1546 |