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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 90 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 51 |
| AluBI | AGCT | 2 cut(s) | 71, 121 |
| AluI | AGCT | 2 cut(s) | 71, 121 |
| Alw21I | GWGCWC | 1 cut(s) | 73 |
| AsuHPI | GGTGA | 1 cut(s) | 79 |
| BanII | GRGCYC | 1 cut(s) | 73 |
| BauI | CACGAG | 1 cut(s) | 72 |
| Bbv12I | GWGCWC | 1 cut(s) | 73 |
| BfaI | CTAG | 1 cut(s) | 157 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 51 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 51 |
| BsiHKAI | GWGCWC | 1 cut(s) | 73 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 51 |
| Bsp1286I | GDGCHC | 1 cut(s) | 73 |
| Bsp143I | GATC | 3 cut(s) | 46, 141, 153 |
| BspACI | CCGC | 1 cut(s) | 90 |
| BssMI | GATC | 3 cut(s) | 46, 141, 153 |
| BssSI | CACGAG | 1 cut(s) | 72 |
| Bst2BI | CACGAG | 1 cut(s) | 72 |
| BstKTI | GATC | 3 cut(s) | 49, 144, 156 |
| BstMBI | GATC | 3 cut(s) | 46, 141, 153 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 118 |
| CviJI | RGCY | 2 cut(s) | 71, 121 |
| CviKI_1 | RGCY | 2 cut(s) | 71, 121 |
| DpnI | GATC | 3 cut(s) | 48, 143, 155 |
| DpnII | GATC | 3 cut(s) | 46, 141, 153 |
| Ecl136II | GAGCTC | 1 cut(s) | 71 |
| Eco24I | GRGCYC | 1 cut(s) | 73 |
| Eco53kI | GAGCTC | 1 cut(s) | 71 |
| EcoICRI | GAGCTC | 1 cut(s) | 71 |
| EcoT38I | GRGCYC | 1 cut(s) | 73 |
| FaiI | YATR | 3 cut(s) | 25, 102, 128 |
| FriOI | GRGCYC | 1 cut(s) | 73 |
| FspBI | CTAG | 1 cut(s) | 157 |
| FspEI | CC | 6 cut(s) | 21, 37, 58, 103, 106, 115 |
| HphI | GGTGA | 1 cut(s) | 79 |
| Hpy188I | TCNGA | 1 cut(s) | 46 |
| Hpy188III | TCNNGA | 3 cut(s) | 30, 74, 145 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 118 |
| Kzo9I | GATC | 3 cut(s) | 46, 141, 153 |
| MaeI | CTAG | 1 cut(s) | 157 |
| MaeIII | GTNAC | 1 cut(s) | 85 |
| MalI | GATC | 3 cut(s) | 48, 143, 155 |
| MboI | GATC | 3 cut(s) | 46, 141, 153 |
| MboII | GAAGA | 2 cut(s) | 89, 151 |
| MhlI | GDGCHC | 1 cut(s) | 73 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 118 |
| NdeII | GATC | 3 cut(s) | 46, 141, 153 |
| NmuCI | GTSAC | 1 cut(s) | 85 |
| Psp124BI | GAGCTC | 1 cut(s) | 73 |
| SacI | GAGCTC | 1 cut(s) | 73 |
| Sau3AI | GATC | 3 cut(s) | 46, 141, 153 |
| SduI | GDGCHC | 1 cut(s) | 73 |
| SetI | ASST | 2 cut(s) | 73, 123 |
| SgeI | CNNG | 6 cut(s) | 42, 84, 86, 95, 125, 148 |
| SsiI | CCGC | 1 cut(s) | 90 |
| SspMI | CTAG | 1 cut(s) | 157 |
| SstI | GAGCTC | 1 cut(s) | 73 |
| TaqI | TCGA | 1 cut(s) | 31 |
| TseFI | GTSAC | 1 cut(s) | 85 |
| Tsp45I | GTSAC | 1 cut(s) | 85 |
| XspI | CTAG | 1 cut(s) | 157 |