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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 1497 |
| AccB1I | GGYRCC | 3 cut(s) | 915, 1073, 1497 |
| AccI | GTMKAC | 1 cut(s) | 944 |
| AccIII | TCCGGA | 1 cut(s) | 880 |
| AciI | CCGC | 5 cut(s) | 79, 319, 592, 994, 1422 |
| AclWI | GGATC | 3 cut(s) | 782, 852, 956 |
| AcoI | YGGCCR | 2 cut(s) | 1060, 1209 |
| AcsI | RAATTY | 3 cut(s) | 138, 172, 659 |
| AcyI | GRCGYC | 2 cut(s) | 916, 1074 |
| AfaI | GTAC | 2 cut(s) | 479, 1499 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1224, 1517, 1532 |
| AgsI | TTSAA | 4 cut(s) | 178, 607, 855, 1252 |
| AhlI | ACTAGT | 1 cut(s) | 452 |
| AjiI | CACGTC | 1 cut(s) | 376 |
| AjnI | CCWGG | 2 cut(s) | 369, 1412 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 519, 551, 1244, 1276 |
| AluBI | AGCT | 6 cut(s) | 195, 395, 401, 793, 803, 1196 |
| AluI | AGCT | 6 cut(s) | 195, 395, 401, 793, 803, 1196 |
| Alw21I | GWGCWC | 2 cut(s) | 795, 1198 |
| Alw26I | GTCTC | 3 cut(s) | 523, 727, 1308 |
| AlwI | GGATC | 3 cut(s) | 782, 852, 956 |
| Ama87I | CYCGRG | 1 cut(s) | 359 |
| Aor13HI | TCCGGA | 1 cut(s) | 880 |
| AoxI | GGCC | 4 cut(s) | 1060, 1209, 1215, 1238 |
| ApeKI | GCWGC | 1 cut(s) | 419 |
| ApoI | RAATTY | 3 cut(s) | 138, 172, 659 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 636 |
| Asp718I | GGTACC | 1 cut(s) | 1497 |
| AspLEI | GCGC | 3 cut(s) | 918, 1076, 1117 |
| AspS9I | GGNCC | 3 cut(s) | 1216, 1239, 1399 |
| AsuC2I | CCSGG | 1 cut(s) | 1320 |
| AvaI | CYCGRG | 1 cut(s) | 359 |
| AvaII | GGWCC | 1 cut(s) | 1399 |
| BaeGI | GKGCMC | 1 cut(s) | 1183 |
| BalI | TGGCCA | 2 cut(s) | 1062, 1211 |
| BanI | GGYRCC | 3 cut(s) | 915, 1073, 1497 |
| BanII | GRGCYC | 2 cut(s) | 795, 1198 |
| BauI | CACGAG | 1 cut(s) | 1197 |
| BbsI | GAAGAC | 2 cut(s) | 504, 707 |
| Bbv12I | GWGCWC | 2 cut(s) | 795, 1198 |
| BbvI | GCAGC | 1 cut(s) | 431 |
| BccI | CCATC | 9 cut(s) | 332, 446, 573, 727, 765, 1101, 1384, 1411, 1459 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 746, 780 |
| BciT130I | CCWGG | 2 cut(s) | 371, 1414 |
| BciVI | GTATCC | 2 cut(s) | 343, 1249 |
| BclI | TGATCA | 1 cut(s) | 1281 |
| BcnI | CCSGG | 1 cut(s) | 1320 |
| BcoDI | GTCTC | 3 cut(s) | 523, 727, 1308 |
| BcuI | ACTAGT | 1 cut(s) | 452 |
| BfaI | CTAG | 6 cut(s) | 51, 366, 453, 504, 569, 1224 |
| BfmI | CTRYAG | 1 cut(s) | 96 |
| BfoI | RGCGCY | 3 cut(s) | 919, 1077, 1118 |
| BfuI | GTATCC | 2 cut(s) | 343, 1249 |
| BisI | GCNGC | 2 cut(s) | 420, 1422 |
| BlsI | GCNGC | 2 cut(s) | 421, 1423 |
| Bme1390I | CCNGG | 3 cut(s) | 371, 1320, 1414 |
| Bme18I | GGWCC | 1 cut(s) | 1399 |
| BmeT110I | CYCGRG | 1 cut(s) | 359 |
| BmgBI | CACGTC | 1 cut(s) | 376 |
| BmgT120I | GGNCC | 3 cut(s) | 1216, 1239, 1399 |
| BmiI | GGNNCC | 6 cut(s) | 917, 1016, 1075, 1218, 1401, 1499 |
| BmrFI | CCNGG | 3 cut(s) | 371, 1320, 1414 |
| BmsI | GCATC | 5 cut(s) | 94, 334, 1037, 1218, 1480 |
| BpiI | GAAGAC | 2 cut(s) | 504, 707 |
| BpmI | CTGGAG | 1 cut(s) | 458 |
| Bpu10I | CCTNAGC | 1 cut(s) | 289 |
| BpuEI | CTTGAG | 4 cut(s) | 74, 314, 381, 1363 |
| BpuMI | CCSGG | 1 cut(s) | 1320 |
| Bsa29I | ATCGAT | 1 cut(s) | 756 |
| BsaHI | GRCGYC | 2 cut(s) | 916, 1074 |
| BsaI | GGTCTC | 1 cut(s) | 523 |
| BsaJI | CCNNGG | 2 cut(s) | 1212, 1242 |
| BsaWI | WCCGGW | 1 cut(s) | 880 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1224, 1517, 1532 |
| Bse1I | ACTGG | 5 cut(s) | 49, 289, 475, 1212, 1537 |
| BseAI | TCCGGA | 1 cut(s) | 880 |
| BseBI | CCWGG | 2 cut(s) | 371, 1414 |
| BseCI | ATCGAT | 1 cut(s) | 756 |
| BseDI | CCNNGG | 2 cut(s) | 1212, 1242 |
| BseGI | GGATG | 6 cut(s) | 246, 441, 469, 1052, 1233, 1451 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1224, 1517, 1532 |
| BseMII | CTCAG | 1 cut(s) | 146 |
| BseNI | ACTGG | 5 cut(s) | 49, 289, 475, 1212, 1537 |
| BseSI | GKGCMC | 1 cut(s) | 1183 |
| BseXI | GCAGC | 1 cut(s) | 431 |
| BseYI | CCCAGC | 1 cut(s) | 1493 |
| BshFI | GGCC | 4 cut(s) | 1062, 1211, 1217, 1240 |
| BshNI | GGYRCC | 3 cut(s) | 915, 1073, 1497 |
| BshVI | ATCGAT | 1 cut(s) | 756 |
| BsiHKAI | GWGCWC | 2 cut(s) | 795, 1198 |
| BsiHKCI | CYCGRG | 1 cut(s) | 359 |
| BsiSI | CCGG | 2 cut(s) | 881, 1319 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1224, 1517, 1532 |
| BsmAI | GTCTC | 3 cut(s) | 523, 727, 1308 |
| BsmBI | CGTCTC | 1 cut(s) | 727 |
| BsmI | GAATGC | 1 cut(s) | 1423 |
| BsnI | GGCC | 4 cut(s) | 1062, 1211, 1217, 1240 |
| Bso31I | GGTCTC | 1 cut(s) | 523 |
| BsoBI | CYCGRG | 1 cut(s) | 359 |
| Bsp1286I | GDGCHC | 3 cut(s) | 795, 1183, 1198 |
| Bsp13I | TCCGGA | 1 cut(s) | 880 |
| Bsp143I | GATC | 5 cut(s) | 675, 774, 857, 961, 1281 |
| Bsp19I | CCATGG | 1 cut(s) | 1212 |
| BspACI | CCGC | 5 cut(s) | 79, 319, 592, 994, 1422 |
| BspANI | GGCC | 4 cut(s) | 1062, 1211, 1217, 1240 |
| BspCNI | CTCAG | 1 cut(s) | 145 |
| BspDI | ATCGAT | 1 cut(s) | 756 |
| BspEI | TCCGGA | 1 cut(s) | 880 |
| BspHI | TCATGA | 2 cut(s) | 787, 1306 |
| BspLI | GGNNCC | 6 cut(s) | 917, 1016, 1075, 1218, 1401, 1499 |
| BspPI | GGATC | 3 cut(s) | 782, 852, 956 |
| BspT107I | GGYRCC | 3 cut(s) | 915, 1073, 1497 |
| BspTNI | GGTCTC | 1 cut(s) | 523 |
| BsrI | ACTGG | 5 cut(s) | 49, 289, 475, 1212, 1537 |
| BssECI | CCNNGG | 2 cut(s) | 1212, 1242 |
| BssMI | GATC | 5 cut(s) | 675, 774, 857, 961, 1281 |
| BssNAI | GTATAC | 1 cut(s) | 945 |
| BssNI | GRCGYC | 2 cut(s) | 916, 1074 |
| BssSI | CACGAG | 1 cut(s) | 1197 |
| BssT1I | CCWWGG | 1 cut(s) | 1212 |
| Bst1107I | GTATAC | 1 cut(s) | 945 |
| Bst2BI | CACGAG | 1 cut(s) | 1197 |
| Bst2UI | CCWGG | 2 cut(s) | 371, 1414 |
| Bst4CI | ACNGT | 1 cut(s) | 385 |
| Bst6I | CTCTTC | 1 cut(s) | 1015 |
| BstACI | GRCGYC | 2 cut(s) | 916, 1074 |
| BstC8I | GCNNGC | 5 cut(s) | 83, 323, 393, 1432, 1436 |
| BstDEI | CTNAG | 9 cut(s) | 132, 167, 289, 424, 741, 906, 938, 1086, 1170 |
| BstDSI | CCRYGG | 2 cut(s) | 1212, 1242 |
| BstEII | GGTNACC | 1 cut(s) | 989 |
| BstF5I | GGATG | 6 cut(s) | 246, 441, 469, 1052, 1233, 1451 |
| BstH2I | RGCGCY | 3 cut(s) | 919, 1077, 1118 |
| BstHHI | GCGC | 3 cut(s) | 918, 1076, 1117 |
| BstKTI | GATC | 5 cut(s) | 678, 777, 860, 964, 1284 |
| BstMAI | GTCTC | 3 cut(s) | 523, 727, 1308 |
| BstMBI | GATC | 5 cut(s) | 675, 774, 857, 961, 1281 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 201, 407, 416, 922, 1112, 1237, 1427 |
| BstNI | CCWGG | 2 cut(s) | 371, 1414 |
| BstNSI | RCATGY | 1 cut(s) | 1438 |
| BstPI | GGTNACC | 1 cut(s) | 989 |
| BstSCI | CCNGG | 3 cut(s) | 369, 1318, 1412 |
| BstSFI | CTRYAG | 1 cut(s) | 96 |
| BstSLI | GKGCMC | 1 cut(s) | 1183 |
| BstV1I | GCAGC | 1 cut(s) | 431 |
| BstV2I | GAAGAC | 2 cut(s) | 504, 707 |
| BstX2I | RGATCY | 1 cut(s) | 857 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1070 |
| BstYI | RGATCY | 1 cut(s) | 857 |
| BstZ17I | GTATAC | 1 cut(s) | 945 |
| Bsu15I | ATCGAT | 1 cut(s) | 756 |
| BsuI | GTATCC | 2 cut(s) | 343, 1249 |
| BsuRI | GGCC | 4 cut(s) | 1062, 1211, 1217, 1240 |
| BsuTUI | ATCGAT | 1 cut(s) | 756 |
| BtgI | CCRYGG | 2 cut(s) | 1212, 1242 |
| BtrI | CACGTC | 1 cut(s) | 376 |
| BtsCI | GGATG | 6 cut(s) | 246, 441, 469, 1052, 1233, 1451 |
| Cac8I | GCNNGC | 5 cut(s) | 83, 323, 393, 1432, 1436 |
| CciI | TCATGA | 2 cut(s) | 787, 1306 |
| CfoI | GCGC | 3 cut(s) | 918, 1076, 1117 |
| Cfr13I | GGNCC | 3 cut(s) | 1216, 1239, 1399 |
| ClaI | ATCGAT | 1 cut(s) | 756 |
| CseI | GACGC | 1 cut(s) | 1112 |
| Csp6I | GTAC | 2 cut(s) | 478, 1498 |
| CviAII | CATG | 6 cut(s) | 788, 824, 1064, 1213, 1307, 1435 |
| CviQI | GTAC | 2 cut(s) | 478, 1498 |
| DdeI | CTNAG | 9 cut(s) | 132, 167, 289, 424, 741, 906, 938, 1086, 1170 |
| DinI | GGCGCC | 2 cut(s) | 917, 1075 |
| DpnI | GATC | 5 cut(s) | 677, 776, 859, 963, 1283 |
| DpnII | GATC | 5 cut(s) | 675, 774, 857, 961, 1281 |
| EaeI | YGGCCR | 2 cut(s) | 1060, 1209 |
| Eam1104I | CTCTTC | 1 cut(s) | 1015 |
| EarI | CTCTTC | 1 cut(s) | 1015 |
| EciI | GGCGGA | 2 cut(s) | 68, 308 |
| Ecl136II | GAGCTC | 2 cut(s) | 793, 1196 |
| Eco130I | CCWWGG | 1 cut(s) | 1212 |
| Eco24I | GRGCYC | 2 cut(s) | 795, 1198 |
| Eco31I | GGTCTC | 1 cut(s) | 523 |
| Eco32I | GATATC | 4 cut(s) | 56, 76, 296, 1330 |
| Eco47I | GGWCC | 1 cut(s) | 1399 |
| Eco53kI | GAGCTC | 2 cut(s) | 793, 1196 |
| Eco88I | CYCGRG | 1 cut(s) | 359 |
| Eco91I | GGTNACC | 1 cut(s) | 989 |
| EcoICRI | GAGCTC | 2 cut(s) | 793, 1196 |
| EcoO65I | GGTNACC | 1 cut(s) | 989 |
| EcoRI | GAATTC | 1 cut(s) | 659 |
| EcoRII | CCWGG | 2 cut(s) | 369, 1412 |
| EcoRV | GATATC | 4 cut(s) | 56, 76, 296, 1330 |
| EcoT14I | CCWWGG | 1 cut(s) | 1212 |
| EcoT38I | GRGCYC | 2 cut(s) | 795, 1198 |
| EgeI | GGCGCC | 2 cut(s) | 917, 1075 |
| EheI | GGCGCC | 2 cut(s) | 917, 1075 |
| ErhI | CCWWGG | 1 cut(s) | 1212 |
| Esp3I | CGTCTC | 1 cut(s) | 727 |
| FaeI | CATG | 6 cut(s) | 791, 827, 1067, 1216, 1310, 1438 |
| FatI | CATG | 6 cut(s) | 787, 823, 1063, 1212, 1306, 1434 |
| FauNDI | CATATG | 1 cut(s) | 459 |
| FbaI | TGATCA | 1 cut(s) | 1281 |
| FblI | GTMKAC | 1 cut(s) | 944 |
| Fnu4HI | GCNGC | 2 cut(s) | 420, 1422 |
| FokI | GGATG | 6 cut(s) | 253, 428, 476, 1059, 1240, 1438 |
| FriOI | GRGCYC | 2 cut(s) | 795, 1198 |
| Fsp4HI | GCNGC | 2 cut(s) | 420, 1422 |
| FspBI | CTAG | 6 cut(s) | 51, 366, 453, 504, 569, 1224 |
| GlaI | GCGC | 3 cut(s) | 917, 1075, 1116 |
| GluI | GCNGC | 2 cut(s) | 420, 1422 |
| GsaI | CCCAGC | 1 cut(s) | 1497 |
| GsuI | CTGGAG | 1 cut(s) | 458 |
| HaeII | RGCGCY | 3 cut(s) | 919, 1077, 1118 |
| HaeIII | GGCC | 4 cut(s) | 1062, 1211, 1217, 1240 |
| HapII | CCGG | 2 cut(s) | 881, 1319 |
| HgaI | GACGC | 1 cut(s) | 1112 |
| HhaI | GCGC | 3 cut(s) | 918, 1076, 1117 |
| Hin1I | GRCGYC | 2 cut(s) | 916, 1074 |
| Hin1II | CATG | 6 cut(s) | 791, 827, 1067, 1216, 1310, 1438 |
| Hin6I | GCGC | 3 cut(s) | 916, 1074, 1115 |
| HinP1I | GCGC | 3 cut(s) | 916, 1074, 1115 |
| HindIII | AAGCTT | 1 cut(s) | 399 |
| HpaII | CCGG | 2 cut(s) | 881, 1319 |
| Hpy166II | GTNNAC | 2 cut(s) | 945, 1028 |
| Hpy188I | TCNGA | 5 cut(s) | 135, 208, 631, 961, 1399 |
| Hpy8I | GTNNAC | 2 cut(s) | 945, 1028 |
| HpyAV | CCTTC | 5 cut(s) | 501, 724, 980, 1246, 1472 |
| HpyCH4III | ACNGT | 1 cut(s) | 385 |
| HpyCH4IV | ACGT | 3 cut(s) | 375, 543, 1030 |
| HpyCH4V | TGCA | 3 cut(s) | 728, 767, 1050 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 201, 407, 416, 922, 1112, 1237, 1427 |
| HpyF3I | CTNAG | 9 cut(s) | 132, 167, 289, 424, 741, 906, 938, 1086, 1170 |
| HpySE526I | ACGT | 3 cut(s) | 375, 543, 1030 |
| Hsp92I | GRCGYC | 2 cut(s) | 916, 1074 |
| Hsp92II | CATG | 6 cut(s) | 791, 827, 1067, 1216, 1310, 1438 |
| HspAI | GCGC | 3 cut(s) | 916, 1074, 1115 |
| KasI | GGCGCC | 2 cut(s) | 915, 1073 |
| Kpn2I | TCCGGA | 1 cut(s) | 880 |
| KpnI | GGTACC | 1 cut(s) | 1501 |
| Ksp22I | TGATCA | 1 cut(s) | 1281 |
| Kzo9I | GATC | 5 cut(s) | 675, 774, 857, 961, 1281 |
| LmnI | GCTCC | 4 cut(s) | 209, 274, 477, 800 |
| Lsp1109I | GCAGC | 1 cut(s) | 431 |
| LweI | GCATC | 5 cut(s) | 94, 334, 1037, 1218, 1480 |
| MaeI | CTAG | 6 cut(s) | 51, 366, 453, 504, 569, 1224 |
| MaeII | ACGT | 3 cut(s) | 375, 543, 1030 |
| MaeIII | GTNAC | 4 cut(s) | 5, 245, 548, 989 |
| MalI | GATC | 5 cut(s) | 677, 776, 859, 963, 1283 |
| MboI | GATC | 5 cut(s) | 675, 774, 857, 961, 1281 |
| MboII | GAAGA | 8 cut(s) | 506, 509, 539, 712, 853, 1032, 1214, 1307 |
| MflI | RGATCY | 1 cut(s) | 857 |
| MhlI | GDGCHC | 3 cut(s) | 795, 1183, 1198 |
| MlsI | TGGCCA | 2 cut(s) | 1062, 1211 |
| MluNI | TGGCCA | 2 cut(s) | 1062, 1211 |
| Mly113I | GGCGCC | 2 cut(s) | 916, 1074 |
| MlyI | GAGTC | 3 cut(s) | 545, 697, 1285 |
| Mox20I | TGGCCA | 2 cut(s) | 1062, 1211 |
| MroI | TCCGGA | 1 cut(s) | 880 |
| MroXI | GAANNNNTTC | 1 cut(s) | 636 |
| MscI | TGGCCA | 2 cut(s) | 1062, 1211 |
| MseI | TTAA | 4 cut(s) | 645, 653, 692, 1537 |
| MslI | CAYNNNNRTG | 4 cut(s) | 446, 462, 1068, 1098 |
| Msp20I | TGGCCA | 2 cut(s) | 1062, 1211 |
| MspI | CCGG | 2 cut(s) | 881, 1319 |
| MspR9I | CCNGG | 3 cut(s) | 371, 1320, 1414 |
| Mva1269I | GAATGC | 1 cut(s) | 1423 |
| MvaI | CCWGG | 2 cut(s) | 371, 1414 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 201, 407, 416, 922, 1112, 1237, 1427 |
| NarI | GGCGCC | 2 cut(s) | 916, 1074 |
| NciI | CCSGG | 1 cut(s) | 1320 |
| NcoI | CCATGG | 1 cut(s) | 1212 |
| NdeI | CATATG | 1 cut(s) | 459 |
| NdeII | GATC | 5 cut(s) | 675, 774, 857, 961, 1281 |
| NlaIII | CATG | 6 cut(s) | 791, 827, 1067, 1216, 1310, 1438 |
| NlaIV | GGNNCC | 6 cut(s) | 917, 1016, 1075, 1218, 1401, 1499 |
| NmuCI | GTSAC | 1 cut(s) | 548 |
| NspI | RCATGY | 1 cut(s) | 1438 |
| PaeI | GCATGC | 1 cut(s) | 1438 |
| PaeR7I | CTCGAG | 1 cut(s) | 359 |
| PagI | TCATGA | 2 cut(s) | 787, 1306 |
| PctI | GAATGC | 1 cut(s) | 1423 |
| PdmI | GAANNNNTTC | 1 cut(s) | 636 |
| PfeI | GAWTC | 7 cut(s) | 102, 342, 355, 467, 753, 884, 1394 |
| PkrI | GCNGC | 2 cut(s) | 421, 1423 |
| PleI | GAGTC | 3 cut(s) | 545, 697, 1285 |
| PluTI | GGCGCC | 2 cut(s) | 919, 1077 |
| PpsI | GAGTC | 3 cut(s) | 545, 697, 1285 |
| Psp124BI | GAGCTC | 2 cut(s) | 795, 1198 |
| Psp6I | CCWGG | 2 cut(s) | 369, 1412 |
| PspEI | GGTNACC | 1 cut(s) | 989 |
| PspFI | CCCAGC | 1 cut(s) | 1493 |
| PspGI | CCWGG | 2 cut(s) | 369, 1412 |
| PspN4I | GGNNCC | 6 cut(s) | 917, 1016, 1075, 1218, 1401, 1499 |
| PspPI | GGNCC | 3 cut(s) | 1216, 1239, 1399 |
| PsuI | RGATCY | 1 cut(s) | 857 |
| RsaI | GTAC | 2 cut(s) | 479, 1499 |
| RsaNI | GTAC | 2 cut(s) | 478, 1498 |
| RseI | CAYNNNNRTG | 4 cut(s) | 446, 462, 1068, 1098 |
| SacI | GAGCTC | 2 cut(s) | 795, 1198 |
| SaqAI | TTAA | 4 cut(s) | 645, 653, 692, 1537 |
| SatI | GCNGC | 2 cut(s) | 420, 1422 |
| Sau3AI | GATC | 5 cut(s) | 675, 774, 857, 961, 1281 |
| Sau96I | GGNCC | 3 cut(s) | 1216, 1239, 1399 |
| SchI | GAGTC | 3 cut(s) | 545, 697, 1285 |
| ScrFI | CCNGG | 3 cut(s) | 371, 1320, 1414 |
| SduI | GDGCHC | 3 cut(s) | 795, 1183, 1198 |
| SfaNI | GCATC | 5 cut(s) | 94, 334, 1037, 1218, 1480 |
| SfcI | CTRYAG | 1 cut(s) | 96 |
| SfoI | GGCGCC | 2 cut(s) | 917, 1075 |
| Sfr274I | CTCGAG | 1 cut(s) | 359 |
| SinI | GGWCC | 1 cut(s) | 1399 |
| SlaI | CTCGAG | 1 cut(s) | 359 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 446, 462, 1068, 1098 |
| SmlI | CTYRAG | 5 cut(s) | 89, 329, 359, 396, 1342 |
| SmoI | CTYRAG | 5 cut(s) | 89, 329, 359, 396, 1342 |
| SpeI | ACTAGT | 1 cut(s) | 452 |
| SphI | GCATGC | 1 cut(s) | 1438 |
| SsiI | CCGC | 5 cut(s) | 79, 319, 592, 994, 1422 |
| SspDI | GGCGCC | 2 cut(s) | 915, 1073 |
| SspMI | CTAG | 6 cut(s) | 51, 366, 453, 504, 569, 1224 |
| SstI | GAGCTC | 2 cut(s) | 795, 1198 |
| StyD4I | CCNGG | 3 cut(s) | 369, 1318, 1412 |
| StyI | CCWWGG | 1 cut(s) | 1212 |
| TaaI | ACNGT | 1 cut(s) | 385 |
| TaiI | ACGT | 3 cut(s) | 378, 546, 1033 |
| TaqI | TCGA | 8 cut(s) | 31, 360, 525, 751, 756, 1134, 1156, 1336 |
| TauI | GCSGC | 1 cut(s) | 1424 |
| TfiI | GAWTC | 7 cut(s) | 102, 342, 355, 467, 753, 884, 1394 |
| Tru1I | TTAA | 4 cut(s) | 645, 653, 692, 1537 |
| Tru9I | TTAA | 4 cut(s) | 645, 653, 692, 1537 |
| TseFI | GTSAC | 1 cut(s) | 548 |
| TseI | GCWGC | 1 cut(s) | 419 |
| Tsp45I | GTSAC | 1 cut(s) | 548 |
| TspDTI | ATGAA | 4 cut(s) | 503, 507, 776, 821 |
| TspGWI | ACGGA | 3 cut(s) | 536, 850, 1027 |
| VpaK11BI | GGWCC | 1 cut(s) | 1399 |
| XapI | RAATTY | 3 cut(s) | 138, 172, 659 |
| XbaI | TCTAGA | 1 cut(s) | 50 |
| XceI | RCATGY | 1 cut(s) | 1438 |
| XhoI | CTCGAG | 1 cut(s) | 359 |
| XmiI | GTMKAC | 1 cut(s) | 944 |
| XmnI | GAANNNNTTC | 1 cut(s) | 636 |
| XspI | CTAG | 6 cut(s) | 51, 366, 453, 504, 569, 1224 |