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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 419 |
| AccB1I | GGYRCC | 4 cut(s) | 155, 1290, 1584, 1742 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 362 |
| AccII | CGCG | 3 cut(s) | 22, 90, 95 |
| AccIII | TCCGGA | 2 cut(s) | 1255, 1549 |
| AclWI | GGATC | 4 cut(s) | 1157, 1227, 1451, 1521 |
| AcoI | YGGCCR | 3 cut(s) | 224, 513, 1729 |
| AcsI | RAATTY | 3 cut(s) | 300, 1034, 1369 |
| AcuI | CTGAAG | 4 cut(s) | 222, 252, 262, 605 |
| AcyI | GRCGYC | 4 cut(s) | 133, 156, 1291, 1585 |
| AfaI | GTAC | 1 cut(s) | 637 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 279, 362, 1286, 1580, 1755 |
| AgeI | ACCGGT | 1 cut(s) | 264 |
| AgsI | TTSAA | 3 cut(s) | 37, 982, 1780 |
| AjiI | CACGTC | 2 cut(s) | 575, 600 |
| AjnI | CCWGG | 2 cut(s) | 151, 786 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 474, 506 |
| Alw26I | GTCTC | 1 cut(s) | 10 |
| AlwI | GGATC | 4 cut(s) | 1157, 1227, 1451, 1521 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 341 |
| Ama87I | CYCGRG | 1 cut(s) | 776 |
| Aor13HI | TCCGGA | 2 cut(s) | 1255, 1549 |
| AoxI | GGCC | 8 cut(s) | 204, 224, 469, 477, 494, 513, 527, 1729 |
| ApeKI | GCWGC | 1 cut(s) | 836 |
| ApoI | RAATTY | 3 cut(s) | 300, 1034, 1369 |
| AsiGI | ACCGGT | 1 cut(s) | 264 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1011 |
| AspLEI | GCGC | 7 cut(s) | 24, 90, 95, 158, 627, 1293, 1587 |
| AspS9I | GGNCC | 6 cut(s) | 127, 470, 478, 495, 1337, 1631 |
| AsuHPI | GGTGA | 2 cut(s) | 262, 542 |
| AvaI | CYCGRG | 1 cut(s) | 776 |
| AvaII | GGWCC | 3 cut(s) | 127, 1337, 1631 |
| BalI | TGGCCA | 2 cut(s) | 226, 1731 |
| BanI | GGYRCC | 4 cut(s) | 155, 1290, 1584, 1742 |
| BanII | GRGCYC | 1 cut(s) | 646 |
| BbsI | GAAGAC | 2 cut(s) | 25, 1082 |
| BbvI | GCAGC | 1 cut(s) | 848 |
| BccI | CCATC | 8 cut(s) | 896, 948, 1102, 1103, 1140, 1396, 1434, 1770 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1121, 1155, 1415, 1449 |
| BciT130I | CCWGG | 2 cut(s) | 153, 788 |
| BciVI | GTATCC | 2 cut(s) | 1312, 1606 |
| BcoDI | GTCTC | 1 cut(s) | 10 |
| BfaI | CTAG | 7 cut(s) | 197, 368, 531, 554, 764, 944, 1755 |
| BfmI | CTRYAG | 3 cut(s) | 142, 1140, 1434 |
| BfoI | RGCGCY | 4 cut(s) | 159, 628, 1294, 1588 |
| BfuAI | ACCTGC | 1 cut(s) | 419 |
| BfuI | GTATCC | 2 cut(s) | 1312, 1606 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 153, 281, 845 |
| BisI | GCNGC | 5 cut(s) | 91, 101, 186, 477, 837 |
| BlsI | GCNGC | 5 cut(s) | 92, 102, 187, 478, 838 |
| Bme1390I | CCNGG | 2 cut(s) | 153, 788 |
| Bme18I | GGWCC | 3 cut(s) | 127, 1337, 1631 |
| BmeT110I | CYCGRG | 1 cut(s) | 776 |
| BmgBI | CACGTC | 2 cut(s) | 575, 600 |
| BmgT120I | GGNCC | 6 cut(s) | 127, 470, 478, 495, 1337, 1631 |
| BmiI | GGNNCC | 5 cut(s) | 157, 1292, 1586, 1744, 1760 |
| BmrFI | CCNGG | 2 cut(s) | 153, 788 |
| BmsI | GCATC | 4 cut(s) | 36, 66, 751, 1048 |
| BpiI | GAAGAC | 2 cut(s) | 25, 1082 |
| BpmI | CTGGAG | 1 cut(s) | 140 |
| Bpu10I | CCTNAGC | 4 cut(s) | 1308, 1313, 1602, 1607 |
| BpuEI | CTTGAG | 2 cut(s) | 731, 798 |
| Bsa29I | ATCGAT | 2 cut(s) | 1131, 1425 |
| BsaHI | GRCGYC | 4 cut(s) | 133, 156, 1291, 1585 |
| BsaI | GGTCTC | 1 cut(s) | 10 |
| BsaJI | CCNNGG | 2 cut(s) | 136, 466 |
| BsaWI | WCCGGW | 3 cut(s) | 264, 1255, 1549 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 1790 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 279, 362, 1286, 1580, 1755 |
| Bse118I | RCCGGY | 3 cut(s) | 181, 264, 511 |
| Bse1I | ACTGG | 4 cut(s) | 227, 346, 584, 706 |
| BseAI | TCCGGA | 2 cut(s) | 1255, 1549 |
| BseBI | CCWGG | 2 cut(s) | 153, 788 |
| BseCI | ATCGAT | 2 cut(s) | 1131, 1425 |
| BseDI | CCNNGG | 2 cut(s) | 136, 466 |
| BseGI | GGATG | 9 cut(s) | 568, 663, 891, 1151, 1206, 1334, 1445, 1500, 1628 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 279, 362, 1286, 1580, 1755 |
| BseMII | CTCAG | 1 cut(s) | 71 |
| BseNI | ACTGG | 4 cut(s) | 227, 346, 584, 706 |
| BseXI | GCAGC | 1 cut(s) | 848 |
| BseYI | CCCAGC | 2 cut(s) | 24, 472 |
| Bsh1236I | CGCG | 3 cut(s) | 22, 90, 95 |
| BshFI | GGCC | 8 cut(s) | 206, 226, 471, 479, 496, 515, 529, 1731 |
| BshNI | GGYRCC | 4 cut(s) | 155, 1290, 1584, 1742 |
| BshTI | ACCGGT | 1 cut(s) | 264 |
| BshVI | ATCGAT | 2 cut(s) | 1131, 1425 |
| BsiHKCI | CYCGRG | 1 cut(s) | 776 |
| BsiSI | CCGG | 6 cut(s) | 182, 207, 265, 512, 1256, 1550 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 279, 362, 1286, 1580, 1755 |
| BsmAI | GTCTC | 1 cut(s) | 10 |
| BsnI | GGCC | 8 cut(s) | 206, 226, 471, 479, 496, 515, 529, 1731 |
| Bso31I | GGTCTC | 1 cut(s) | 10 |
| BsoBI | CYCGRG | 1 cut(s) | 776 |
| Bsp1286I | GDGCHC | 1 cut(s) | 646 |
| Bsp13I | TCCGGA | 2 cut(s) | 1255, 1549 |
| Bsp143I | GATC | 7 cut(s) | 115, 940, 1050, 1149, 1232, 1443, 1526 |
| BspANI | GGCC | 8 cut(s) | 206, 226, 471, 479, 496, 515, 529, 1731 |
| BspCNI | CTCAG | 1 cut(s) | 70 |
| BspDI | ATCGAT | 2 cut(s) | 1131, 1425 |
| BspEI | TCCGGA | 2 cut(s) | 1255, 1549 |
| BspFNI | CGCG | 3 cut(s) | 22, 90, 95 |
| BspHI | TCATGA | 2 cut(s) | 1162, 1456 |
| BspLI | GGNNCC | 5 cut(s) | 157, 1292, 1586, 1744, 1760 |
| BspMAI | CTGCAG | 2 cut(s) | 1144, 1438 |
| BspMI | ACCTGC | 1 cut(s) | 419 |
| BspPI | GGATC | 4 cut(s) | 1157, 1227, 1451, 1521 |
| BspT107I | GGYRCC | 4 cut(s) | 155, 1290, 1584, 1742 |
| BspTNI | GGTCTC | 1 cut(s) | 10 |
| BsrFI | RCCGGY | 3 cut(s) | 181, 264, 511 |
| BsrI | ACTGG | 4 cut(s) | 227, 346, 584, 706 |
| BssAI | RCCGGY | 3 cut(s) | 181, 264, 511 |
| BssECI | CCNNGG | 2 cut(s) | 136, 466 |
| BssMI | GATC | 7 cut(s) | 115, 940, 1050, 1149, 1232, 1443, 1526 |
| BssNI | GRCGYC | 4 cut(s) | 133, 156, 1291, 1585 |
| BssT1I | CCWWGG | 1 cut(s) | 466 |
| Bst2UI | CCWGG | 2 cut(s) | 153, 788 |
| Bst4CI | ACNGT | 1 cut(s) | 802 |
| Bst6I | CTCTTC | 2 cut(s) | 535, 1684 |
| BstACI | GRCGYC | 4 cut(s) | 133, 156, 1291, 1585 |
| BstC8I | GCNNGC | 3 cut(s) | 149, 740, 810 |
| BstEII | GGTNACC | 1 cut(s) | 1658 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1284, 1578 |
| BstF5I | GGATG | 9 cut(s) | 568, 663, 891, 1151, 1206, 1334, 1445, 1500, 1628 |
| BstFNI | CGCG | 3 cut(s) | 22, 90, 95 |
| BstH2I | RGCGCY | 4 cut(s) | 159, 628, 1294, 1588 |
| BstHHI | GCGC | 7 cut(s) | 24, 90, 95, 158, 627, 1293, 1587 |
| BstKTI | GATC | 7 cut(s) | 118, 943, 1053, 1152, 1235, 1446, 1529 |
| BstMAI | GTCTC | 1 cut(s) | 10 |
| BstMBI | GATC | 7 cut(s) | 115, 940, 1050, 1149, 1232, 1443, 1526 |
| BstNI | CCWGG | 2 cut(s) | 153, 788 |
| BstNSI | RCATGY | 1 cut(s) | 796 |
| BstPI | GGTNACC | 1 cut(s) | 1658 |
| BstSCI | CCNGG | 2 cut(s) | 151, 786 |
| BstSFI | CTRYAG | 3 cut(s) | 142, 1140, 1434 |
| BstUI | CGCG | 3 cut(s) | 22, 90, 95 |
| BstV1I | GCAGC | 1 cut(s) | 848 |
| BstV2I | GAAGAC | 2 cut(s) | 25, 1082 |
| BstX2I | RGATCY | 2 cut(s) | 1232, 1526 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1739 |
| BstYI | RGATCY | 2 cut(s) | 1232, 1526 |
| Bsu15I | ATCGAT | 2 cut(s) | 1131, 1425 |
| BsuI | GTATCC | 2 cut(s) | 1312, 1606 |
| BsuRI | GGCC | 8 cut(s) | 206, 226, 471, 479, 496, 515, 529, 1731 |
| BsuTUI | ATCGAT | 2 cut(s) | 1131, 1425 |
| BtgZI | GCGATG | 1 cut(s) | 1076 |
| BtrI | CACGTC | 2 cut(s) | 575, 600 |
| BtsCI | GGATG | 9 cut(s) | 568, 663, 891, 1151, 1206, 1334, 1445, 1500, 1628 |
| BtsIMutI | CAGTG | 1 cut(s) | 1482 |
| BveI | ACCTGC | 1 cut(s) | 419 |
| Cac8I | GCNNGC | 3 cut(s) | 149, 740, 810 |
| CaiI | CAGNNNCTG | 1 cut(s) | 341 |
| CciI | TCATGA | 2 cut(s) | 1162, 1456 |
| CfoI | GCGC | 7 cut(s) | 24, 90, 95, 158, 627, 1293, 1587 |
| Cfr10I | RCCGGY | 3 cut(s) | 181, 264, 511 |
| Cfr13I | GGNCC | 6 cut(s) | 127, 470, 478, 495, 1337, 1631 |
| ClaI | ATCGAT | 2 cut(s) | 1131, 1425 |
| CpoI | CGGWCCG | 1 cut(s) | 127 |
| CseI | GACGC | 3 cut(s) | 141, 580, 1781 |
| Csp6I | GTAC | 1 cut(s) | 636 |
| CspAI | ACCGGT | 1 cut(s) | 264 |
| CspI | CGGWCCG | 1 cut(s) | 127 |
| CviAII | CATG | 7 cut(s) | 793, 1163, 1199, 1457, 1493, 1700, 1733 |
| CviQI | GTAC | 1 cut(s) | 636 |
| DinI | GGCGCC | 3 cut(s) | 157, 1292, 1586 |
| DpnI | GATC | 7 cut(s) | 117, 942, 1052, 1151, 1234, 1445, 1528 |
| DpnII | GATC | 7 cut(s) | 115, 940, 1050, 1149, 1232, 1443, 1526 |
| EaeI | YGGCCR | 3 cut(s) | 224, 513, 1729 |
| Eam1104I | CTCTTC | 2 cut(s) | 535, 1684 |
| EarI | CTCTTC | 2 cut(s) | 535, 1684 |
| EciI | GGCGGA | 2 cut(s) | 603, 725 |
| Eco130I | CCWWGG | 1 cut(s) | 466 |
| Eco24I | GRGCYC | 1 cut(s) | 646 |
| Eco31I | GGTCTC | 1 cut(s) | 10 |
| Eco32I | GATATC | 1 cut(s) | 713 |
| Eco47I | GGWCC | 3 cut(s) | 127, 1337, 1631 |
| Eco57I | CTGAAG | 4 cut(s) | 222, 252, 262, 605 |
| Eco88I | CYCGRG | 1 cut(s) | 776 |
| Eco91I | GGTNACC | 1 cut(s) | 1658 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1284, 1578 |
| EcoO65I | GGTNACC | 1 cut(s) | 1658 |
| EcoRI | GAATTC | 2 cut(s) | 1034, 1369 |
| EcoRII | CCWGG | 2 cut(s) | 151, 786 |
| EcoRV | GATATC | 1 cut(s) | 713 |
| EcoT14I | CCWWGG | 1 cut(s) | 466 |
| EcoT38I | GRGCYC | 1 cut(s) | 646 |
| EgeI | GGCGCC | 3 cut(s) | 157, 1292, 1586 |
| EheI | GGCGCC | 3 cut(s) | 157, 1292, 1586 |
| ErhI | CCWWGG | 1 cut(s) | 466 |
| FaeI | CATG | 7 cut(s) | 796, 1166, 1202, 1460, 1496, 1703, 1736 |
| FatI | CATG | 7 cut(s) | 792, 1162, 1198, 1456, 1492, 1699, 1732 |
| FauI | CCCGC | 2 cut(s) | 156, 255 |
| Fnu4HI | GCNGC | 5 cut(s) | 91, 101, 186, 477, 837 |
| FokI | GGATG | 9 cut(s) | 555, 670, 878, 1158, 1193, 1341, 1452, 1487, 1635 |
| FriOI | GRGCYC | 1 cut(s) | 646 |
| Fsp4HI | GCNGC | 5 cut(s) | 91, 101, 186, 477, 837 |
| FspBI | CTAG | 7 cut(s) | 197, 368, 531, 554, 764, 944, 1755 |
| GlaI | GCGC | 7 cut(s) | 23, 89, 94, 157, 626, 1292, 1586 |
| GluI | GCNGC | 5 cut(s) | 91, 101, 186, 477, 837 |
| GsaI | CCCAGC | 2 cut(s) | 28, 476 |
| GsuI | CTGGAG | 1 cut(s) | 140 |
| HaeII | RGCGCY | 4 cut(s) | 159, 628, 1294, 1588 |
| HaeIII | GGCC | 8 cut(s) | 206, 226, 471, 479, 496, 515, 529, 1731 |
| HapII | CCGG | 6 cut(s) | 182, 207, 265, 512, 1256, 1550 |
| HgaI | GACGC | 3 cut(s) | 141, 580, 1781 |
| HhaI | GCGC | 7 cut(s) | 24, 90, 95, 158, 627, 1293, 1587 |
| Hin1I | GRCGYC | 4 cut(s) | 133, 156, 1291, 1585 |
| Hin1II | CATG | 7 cut(s) | 796, 1166, 1202, 1460, 1496, 1703, 1736 |
| Hin6I | GCGC | 7 cut(s) | 22, 88, 93, 156, 625, 1291, 1585 |
| HinP1I | GCGC | 7 cut(s) | 22, 88, 93, 156, 625, 1291, 1585 |
| HincII | GTYRAC | 2 cut(s) | 578, 1697 |
| HindII | GTYRAC | 2 cut(s) | 578, 1697 |
| HindIII | AAGCTT | 5 cut(s) | 68, 234, 816, 1310, 1604 |
| HpaII | CCGG | 6 cut(s) | 182, 207, 265, 512, 1256, 1550 |
| HphI | GGTGA | 2 cut(s) | 262, 542 |
| Hpy166II | GTNNAC | 4 cut(s) | 270, 509, 578, 1697 |
| Hpy188I | TCNGA | 8 cut(s) | 45, 66, 75, 131, 241, 657, 976, 1006 |
| Hpy8I | GTNNAC | 4 cut(s) | 270, 509, 578, 1697 |
| Hpy99I | CGWCG | 3 cut(s) | 110, 135, 596 |
| HpyAV | CCTTC | 5 cut(s) | 286, 421, 1099, 1393, 1649 |
| HpyCH4III | ACNGT | 1 cut(s) | 802 |
| HpyCH4IV | ACGT | 4 cut(s) | 574, 580, 599, 918 |
| HpyCH4V | TGCA | 5 cut(s) | 414, 1103, 1142, 1397, 1436 |
| HpySE526I | ACGT | 4 cut(s) | 574, 580, 599, 918 |
| Hsp92I | GRCGYC | 4 cut(s) | 133, 156, 1291, 1585 |
| Hsp92II | CATG | 7 cut(s) | 796, 1166, 1202, 1460, 1496, 1703, 1736 |
| HspAI | GCGC | 7 cut(s) | 22, 88, 93, 156, 625, 1291, 1585 |
| KasI | GGCGCC | 3 cut(s) | 155, 1290, 1584 |
| Kpn2I | TCCGGA | 2 cut(s) | 1255, 1549 |
| Kzo9I | GATC | 7 cut(s) | 115, 940, 1050, 1149, 1232, 1443, 1526 |
| LmnI | GCTCC | 5 cut(s) | 616, 691, 1175, 1469, 1758 |
| Lsp1109I | GCAGC | 1 cut(s) | 848 |
| LweI | GCATC | 4 cut(s) | 36, 66, 751, 1048 |
| MaeI | CTAG | 7 cut(s) | 197, 368, 531, 554, 764, 944, 1755 |
| MaeII | ACGT | 4 cut(s) | 574, 580, 599, 918 |
| MaeIII | GTNAC | 6 cut(s) | 662, 923, 1135, 1429, 1480, 1658 |
| MalI | GATC | 7 cut(s) | 117, 942, 1052, 1151, 1234, 1445, 1528 |
| MboI | GATC | 7 cut(s) | 115, 940, 1050, 1149, 1232, 1443, 1526 |
| MflI | RGATCY | 2 cut(s) | 1232, 1526 |
| MhlI | GDGCHC | 1 cut(s) | 646 |
| MlsI | TGGCCA | 2 cut(s) | 226, 1731 |
| MluNI | TGGCCA | 2 cut(s) | 226, 1731 |
| Mly113I | GGCGCC | 3 cut(s) | 156, 1291, 1585 |
| MlyI | GAGTC | 4 cut(s) | 181, 895, 920, 1072 |
| MmeI | TCCRAC | 2 cut(s) | 154, 635 |
| Mox20I | TGGCCA | 2 cut(s) | 226, 1731 |
| MroI | TCCGGA | 2 cut(s) | 1255, 1549 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1011 |
| MscI | TGGCCA | 2 cut(s) | 226, 1731 |
| MseI | TTAA | 4 cut(s) | 1020, 1028, 1224, 1518 |
| MslI | CAYNNNNRTG | 5 cut(s) | 896, 1211, 1505, 1737, 1767 |
| Msp20I | TGGCCA | 2 cut(s) | 226, 1731 |
| MspA1I | CMGCKG | 1 cut(s) | 476 |
| MspI | CCGG | 6 cut(s) | 182, 207, 265, 512, 1256, 1550 |
| MspR9I | CCNGG | 2 cut(s) | 153, 788 |
| MteI | GCGCNGCGC | 1 cut(s) | 91 |
| MvaI | CCWGG | 2 cut(s) | 153, 788 |
| MvnI | CGCG | 3 cut(s) | 22, 90, 95 |
| NarI | GGCGCC | 3 cut(s) | 156, 1291, 1585 |
| NdeII | GATC | 7 cut(s) | 115, 940, 1050, 1149, 1232, 1443, 1526 |
| NlaIII | CATG | 7 cut(s) | 796, 1166, 1202, 1460, 1496, 1703, 1736 |
| NlaIV | GGNNCC | 5 cut(s) | 157, 1292, 1586, 1744, 1760 |
| NmeAIII | GCCGAG | 1 cut(s) | 161 |
| NmuCI | GTSAC | 2 cut(s) | 923, 1480 |
| NspI | RCATGY | 1 cut(s) | 796 |
| PaeR7I | CTCGAG | 1 cut(s) | 776 |
| PagI | TCATGA | 2 cut(s) | 1162, 1456 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 111 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1011 |
| PfeI | GAWTC | 7 cut(s) | 332, 759, 772, 1128, 1259, 1422, 1553 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 362 |
| PinAI | ACCGGT | 1 cut(s) | 264 |
| PkrI | GCNGC | 5 cut(s) | 92, 102, 187, 478, 838 |
| PleI | GAGTC | 4 cut(s) | 180, 895, 920, 1072 |
| PluTI | GGCGCC | 3 cut(s) | 159, 1294, 1588 |
| PpsI | GAGTC | 4 cut(s) | 180, 895, 920, 1072 |
| Psp6I | CCWGG | 2 cut(s) | 151, 786 |
| PspEI | GGTNACC | 1 cut(s) | 1658 |
| PspFI | CCCAGC | 2 cut(s) | 24, 472 |
| PspGI | CCWGG | 2 cut(s) | 151, 786 |
| PspN4I | GGNNCC | 5 cut(s) | 157, 1292, 1586, 1744, 1760 |
| PspPI | GGNCC | 6 cut(s) | 127, 470, 478, 495, 1337, 1631 |
| PstI | CTGCAG | 2 cut(s) | 1144, 1438 |
| PstNI | CAGNNNCTG | 1 cut(s) | 341 |
| PsuI | RGATCY | 2 cut(s) | 1232, 1526 |
| RsaI | GTAC | 1 cut(s) | 637 |
| RsaNI | GTAC | 1 cut(s) | 636 |
| RseI | CAYNNNNRTG | 5 cut(s) | 896, 1211, 1505, 1737, 1767 |
| Rsr2I | CGGWCCG | 1 cut(s) | 127 |
| RsrII | CGGWCCG | 1 cut(s) | 127 |
| SaqAI | TTAA | 4 cut(s) | 1020, 1028, 1224, 1518 |
| SatI | GCNGC | 5 cut(s) | 91, 101, 186, 477, 837 |
| Sau3AI | GATC | 7 cut(s) | 115, 940, 1050, 1149, 1232, 1443, 1526 |
| Sau96I | GGNCC | 6 cut(s) | 127, 470, 478, 495, 1337, 1631 |
| SchI | GAGTC | 4 cut(s) | 181, 895, 920, 1072 |
| ScrFI | CCNGG | 2 cut(s) | 153, 788 |
| SduI | GDGCHC | 1 cut(s) | 646 |
| SfaNI | GCATC | 4 cut(s) | 36, 66, 751, 1048 |
| SfcI | CTRYAG | 3 cut(s) | 142, 1140, 1434 |
| SfoI | GGCGCC | 3 cut(s) | 157, 1292, 1586 |
| Sfr274I | CTCGAG | 1 cut(s) | 776 |
| SinI | GGWCC | 3 cut(s) | 127, 1337, 1631 |
| SlaI | CTCGAG | 1 cut(s) | 776 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 896, 1211, 1505, 1737, 1767 |
| SmlI | CTYRAG | 3 cut(s) | 746, 776, 813 |
| SmoI | CTYRAG | 3 cut(s) | 746, 776, 813 |
| SspDI | GGCGCC | 3 cut(s) | 155, 1290, 1584 |
| SspMI | CTAG | 7 cut(s) | 197, 368, 531, 554, 764, 944, 1755 |
| StyD4I | CCNGG | 2 cut(s) | 151, 786 |
| StyI | CCWWGG | 1 cut(s) | 466 |
| TaaI | ACNGT | 1 cut(s) | 802 |
| TaiI | ACGT | 4 cut(s) | 577, 583, 602, 921 |
| TaqI | TCGA | 5 cut(s) | 777, 1126, 1131, 1420, 1425 |
| TatI | WGTACW | 1 cut(s) | 635 |
| TauI | GCSGC | 4 cut(s) | 93, 103, 188, 479 |
| TfiI | GAWTC | 7 cut(s) | 332, 759, 772, 1128, 1259, 1422, 1553 |
| Tru1I | TTAA | 4 cut(s) | 1020, 1028, 1224, 1518 |
| Tru9I | TTAA | 4 cut(s) | 1020, 1028, 1224, 1518 |
| TscAI | CASTG | 1 cut(s) | 1489 |
| TseFI | GTSAC | 2 cut(s) | 923, 1480 |
| TseI | GCWGC | 1 cut(s) | 836 |
| Tsp45I | GTSAC | 2 cut(s) | 923, 1480 |
| TspDTI | ATGAA | 2 cut(s) | 1151, 1445 |
| TspGWI | ACGGA | 3 cut(s) | 911, 1225, 1519 |
| TspRI | CASTG | 1 cut(s) | 1489 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 362 |
| VpaK11BI | GGWCC | 3 cut(s) | 127, 1337, 1631 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1284, 1578 |
| XapI | RAATTY | 3 cut(s) | 300, 1034, 1369 |
| XbaI | TCTAGA | 2 cut(s) | 367, 943 |
| XceI | RCATGY | 1 cut(s) | 796 |
| XhoI | CTCGAG | 1 cut(s) | 776 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1011 |
| XspI | CTAG | 7 cut(s) | 197, 368, 531, 554, 764, 944, 1755 |