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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1266 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 194 |
| AatII | GACGTC | 1 cut(s) | 192 |
| AccB1I | GGYRCC | 1 cut(s) | 1298 |
| AccIII | TCCGGA | 1 cut(s) | 1252 |
| AciI | CCGC | 3 cut(s) | 294, 784, 1440 |
| AclWI | GGATC | 2 cut(s) | 1154, 1224 |
| AcoI | YGGCCR | 3 cut(s) | 207, 314, 1285 |
| AcsI | RAATTY | 1 cut(s) | 1133 |
| AcuI | CTGAAG | 1 cut(s) | 37 |
| AcyI | GRCGYC | 2 cut(s) | 189, 1299 |
| AdeI | CACNNNGTG | 1 cut(s) | 363 |
| AfaI | GTAC | 1 cut(s) | 953 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 115, 465, 471 |
| AgsI | TTSAA | 3 cut(s) | 128, 1081, 1336 |
| AjiI | CACGTC | 3 cut(s) | 239, 587, 662 |
| AjnI | CCWGG | 5 cut(s) | 316, 458, 464, 594, 834 |
| AleI | CACNNNNGTG | 1 cut(s) | 1071 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 8, 40 |
| AluBI | AGCT | 9 cut(s) | 21, 351, 447, 860, 866, 887, 1165, 1175, 1421 |
| AluI | AGCT | 9 cut(s) | 21, 351, 447, 860, 866, 887, 1165, 1175, 1421 |
| Alw21I | GWGCWC | 2 cut(s) | 1167, 1423 |
| Alw26I | GTCTC | 2 cut(s) | 413, 997 |
| AlwI | GGATC | 2 cut(s) | 1154, 1224 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 595 |
| Ama87I | CYCGRG | 3 cut(s) | 448, 621, 645 |
| Aor13HI | TCCGGA | 1 cut(s) | 1252 |
| AoxI | GGCC | 8 cut(s) | 117, 207, 299, 314, 462, 507, 514, 1285 |
| ApeKI | GCWGC | 2 cut(s) | 99, 554 |
| ApoI | RAATTY | 1 cut(s) | 1133 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 467, 499 |
| AseI | ATTAAT | 1 cut(s) | 1349 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1110 |
| AspA2I | CCTAGG | 1 cut(s) | 109 |
| AspLEI | GCGC | 4 cut(s) | 44, 262, 1301, 1342 |
| AspS9I | GGNCC | 7 cut(s) | 118, 299, 462, 507, 515, 625, 649 |
| AsuC2I | CCSGG | 4 cut(s) | 622, 623, 646, 647 |
| AsuHPI | GGTGA | 1 cut(s) | 788 |
| AsuNHI | GCTAGC | 1 cut(s) | 443 |
| AvaI | CYCGRG | 3 cut(s) | 448, 621, 645 |
| AvaII | GGWCC | 2 cut(s) | 625, 649 |
| AvrII | CCTAGG | 1 cut(s) | 109 |
| BaeGI | GKGCMC | 1 cut(s) | 1408 |
| BalI | TGGCCA | 2 cut(s) | 209, 316 |
| BanI | GGYRCC | 1 cut(s) | 1298 |
| BanII | GRGCYC | 2 cut(s) | 1167, 1423 |
| BauI | CACGAG | 1 cut(s) | 1422 |
| BbsI | GAAGAC | 1 cut(s) | 978 |
| Bbv12I | GWGCWC | 2 cut(s) | 1167, 1423 |
| BbvCI | CCTCAGC | 1 cut(s) | 276 |
| BbvI | GCAGC | 2 cut(s) | 86, 566 |
| BccI | CCATC | 9 cut(s) | 209, 259, 394, 538, 689, 797, 920, 1047, 1326 |
| BceAI | ACGGC | 1 cut(s) | 494 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1264, 1298 |
| BciT130I | CCWGG | 5 cut(s) | 318, 460, 466, 596, 836 |
| BcnI | CCSGG | 4 cut(s) | 622, 623, 646, 647 |
| BcoDI | GTCTC | 2 cut(s) | 413, 997 |
| BfmI | CTRYAG | 1 cut(s) | 352 |
| BfoI | RGCGCY | 4 cut(s) | 45, 263, 1302, 1343 |
| BisI | GCNGC | 2 cut(s) | 100, 555 |
| BlnI | CCTAGG | 1 cut(s) | 109 |
| BlsI | GCNGC | 2 cut(s) | 101, 556 |
| Bme1390I | CCNGG | 9 cut(s) | 318, 460, 466, 596, 622, 623, 646, 647, 836 |
| Bme18I | GGWCC | 2 cut(s) | 625, 649 |
| BmeT110I | CYCGRG | 3 cut(s) | 448, 621, 645 |
| BmgBI | CACGTC | 3 cut(s) | 239, 587, 662 |
| BmgT120I | GGNCC | 7 cut(s) | 118, 299, 462, 507, 515, 625, 649 |
| BmiI | GGNNCC | 6 cut(s) | 120, 626, 627, 650, 651, 1300 |
| BmrFI | CCNGG | 9 cut(s) | 318, 460, 466, 596, 622, 623, 646, 647, 836 |
| BmsI | GCATC | 1 cut(s) | 353 |
| BmtI | GCTAGC | 1 cut(s) | 447 |
| BpiI | GAAGAC | 1 cut(s) | 978 |
| BpmI | CTGGAG | 1 cut(s) | 932 |
| Bpu10I | CCTNAGC | 1 cut(s) | 276 |
| BpuEI | CTTGAG | 4 cut(s) | 408, 779, 845, 846 |
| BpuMI | CCSGG | 4 cut(s) | 622, 623, 646, 647 |
| BsaHI | GRCGYC | 2 cut(s) | 189, 1299 |
| BsaI | GGTCTC | 1 cut(s) | 997 |
| BsaJI | CCNNGG | 8 cut(s) | 109, 286, 459, 465, 595, 621, 645, 1233 |
| BsaWI | WCCGGW | 1 cut(s) | 1252 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 8, 38 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 115, 465, 471 |
| Bse1I | ACTGG | 3 cut(s) | 754, 787, 949 |
| BseAI | TCCGGA | 1 cut(s) | 1252 |
| BseBI | CCWGG | 5 cut(s) | 318, 460, 466, 596, 836 |
| BseDI | CCNNGG | 8 cut(s) | 109, 286, 459, 465, 595, 621, 645, 1233 |
| BseGI | GGATG | 5 cut(s) | 433, 711, 915, 943, 1203 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 115, 465, 471 |
| BseMII | CTCAG | 2 cut(s) | 267, 1350 |
| BseNI | ACTGG | 3 cut(s) | 754, 787, 949 |
| BseSI | GKGCMC | 1 cut(s) | 1408 |
| BseXI | GCAGC | 2 cut(s) | 86, 566 |
| BsgI | GTGCAG | 1 cut(s) | 178 |
| BshFI | GGCC | 8 cut(s) | 119, 209, 301, 316, 464, 509, 516, 1287 |
| BshNI | GGYRCC | 1 cut(s) | 1298 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1167, 1423 |
| BsiHKCI | CYCGRG | 3 cut(s) | 448, 621, 645 |
| BsiSI | CCGG | 3 cut(s) | 622, 646, 1253 |
| BslFI | GGGAC | 4 cut(s) | 488, 613, 638, 662 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 115, 465, 471 |
| BsmAI | GTCTC | 2 cut(s) | 413, 997 |
| BsmFI | GGGAC | 4 cut(s) | 488, 613, 638, 662 |
| BsmI | GAATGC | 1 cut(s) | 412 |
| BsnI | GGCC | 8 cut(s) | 119, 209, 301, 316, 464, 509, 516, 1287 |
| Bso31I | GGTCTC | 1 cut(s) | 997 |
| BsoBI | CYCGRG | 3 cut(s) | 448, 621, 645 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1167, 1408, 1423 |
| Bsp13I | TCCGGA | 1 cut(s) | 1252 |
| Bsp143I | GATC | 3 cut(s) | 370, 1146, 1229 |
| Bsp19I | CCATGG | 1 cut(s) | 1233 |
| BspACI | CCGC | 3 cut(s) | 294, 784, 1440 |
| BspANI | GGCC | 8 cut(s) | 119, 209, 301, 316, 464, 509, 516, 1287 |
| BspCNI | CTCAG | 2 cut(s) | 268, 1351 |
| BspEI | TCCGGA | 1 cut(s) | 1252 |
| BspHI | TCATGA | 1 cut(s) | 1159 |
| BspLI | GGNNCC | 6 cut(s) | 120, 626, 627, 650, 651, 1300 |
| BspOI | GCTAGC | 1 cut(s) | 447 |
| BspPI | GGATC | 2 cut(s) | 1154, 1224 |
| BspT107I | GGYRCC | 1 cut(s) | 1298 |
| BspTNI | GGTCTC | 1 cut(s) | 997 |
| BsrI | ACTGG | 3 cut(s) | 754, 787, 949 |
| BssECI | CCNNGG | 8 cut(s) | 109, 286, 459, 465, 595, 621, 645, 1233 |
| BssMI | GATC | 3 cut(s) | 370, 1146, 1229 |
| BssNI | GRCGYC | 2 cut(s) | 189, 1299 |
| BssSI | CACGAG | 1 cut(s) | 1422 |
| BssT1I | CCWWGG | 3 cut(s) | 109, 286, 1233 |
| Bst2BI | CACGAG | 1 cut(s) | 1422 |
| Bst2UI | CCWGG | 5 cut(s) | 318, 460, 466, 596, 836 |
| Bst4CI | ACNGT | 4 cut(s) | 234, 701, 850, 1457 |
| BstACI | GRCGYC | 2 cut(s) | 189, 1299 |
| BstC8I | GCNNGC | 3 cut(s) | 273, 445, 858 |
| BstDEI | CTNAG | 4 cut(s) | 276, 497, 1311, 1359 |
| BstDSI | CCRYGG | 1 cut(s) | 1233 |
| BstEII | GGTNACC | 1 cut(s) | 381 |
| BstF5I | GGATG | 5 cut(s) | 433, 711, 915, 943, 1203 |
| BstH2I | RGCGCY | 4 cut(s) | 45, 263, 1302, 1343 |
| BstHHI | GCGC | 4 cut(s) | 44, 262, 1301, 1342 |
| BstKTI | GATC | 3 cut(s) | 373, 1149, 1232 |
| BstMAI | GTCTC | 2 cut(s) | 413, 997 |
| BstMBI | GATC | 3 cut(s) | 370, 1146, 1229 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 48, 206, 268, 563, 872, 893, 902, 1448 |
| BstNI | CCWGG | 5 cut(s) | 318, 460, 466, 596, 836 |
| BstPI | GGTNACC | 1 cut(s) | 381 |
| BstSCI | CCNGG | 9 cut(s) | 316, 458, 464, 594, 620, 621, 644, 645, 834 |
| BstSFI | CTRYAG | 1 cut(s) | 352 |
| BstSLI | GKGCMC | 1 cut(s) | 1408 |
| BstV1I | GCAGC | 2 cut(s) | 86, 566 |
| BstV2I | GAAGAC | 1 cut(s) | 978 |
| BstX2I | RGATCY | 1 cut(s) | 1229 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 394, 1295 |
| BstYI | RGATCY | 1 cut(s) | 1229 |
| BsuRI | GGCC | 8 cut(s) | 119, 209, 301, 316, 464, 509, 516, 1287 |
| BtgI | CCRYGG | 1 cut(s) | 1233 |
| BtgZI | GCGATG | 1 cut(s) | 413 |
| BtrI | CACGTC | 3 cut(s) | 239, 587, 662 |
| BtsCI | GGATG | 5 cut(s) | 433, 711, 915, 943, 1203 |
| BtsI | GCAGTG | 2 cut(s) | 653, 1028 |
| BtsIMutI | CAGTG | 5 cut(s) | 230, 399, 653, 1028, 1185 |
| Cac8I | GCNNGC | 3 cut(s) | 273, 445, 858 |
| CaiI | CAGNNNCTG | 1 cut(s) | 595 |
| CciI | TCATGA | 1 cut(s) | 1159 |
| CfoI | GCGC | 4 cut(s) | 44, 262, 1301, 1342 |
| Cfr13I | GGNCC | 7 cut(s) | 118, 299, 462, 507, 515, 625, 649 |
| Cfr9I | CCCGGG | 2 cut(s) | 621, 645 |
| Csp6I | GTAC | 1 cut(s) | 952 |
| CviAII | CATG | 6 cut(s) | 227, 611, 1160, 1196, 1234, 1289 |
| CviQI | GTAC | 1 cut(s) | 952 |
| DdeI | CTNAG | 4 cut(s) | 276, 497, 1311, 1359 |
| DinI | GGCGCC | 1 cut(s) | 1300 |
| DpnI | GATC | 3 cut(s) | 372, 1148, 1231 |
| DpnII | GATC | 3 cut(s) | 370, 1146, 1229 |
| DraIII | CACNNNGTG | 1 cut(s) | 363 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 194 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 194 |
| EaeI | YGGCCR | 3 cut(s) | 207, 314, 1285 |
| EciI | GGCGGA | 1 cut(s) | 773 |
| Ecl136II | GAGCTC | 2 cut(s) | 1165, 1421 |
| Eco130I | CCWWGG | 3 cut(s) | 109, 286, 1233 |
| Eco24I | GRGCYC | 2 cut(s) | 1167, 1423 |
| Eco31I | GGTCTC | 1 cut(s) | 997 |
| Eco32I | GATATC | 1 cut(s) | 761 |
| Eco47I | GGWCC | 2 cut(s) | 625, 649 |
| Eco53kI | GAGCTC | 2 cut(s) | 1165, 1421 |
| Eco57I | CTGAAG | 1 cut(s) | 37 |
| Eco88I | CYCGRG | 3 cut(s) | 448, 621, 645 |
| Eco91I | GGTNACC | 1 cut(s) | 381 |
| EcoICRI | GAGCTC | 2 cut(s) | 1165, 1421 |
| EcoO109I | RGGNCCY | 2 cut(s) | 625, 649 |
| EcoO65I | GGTNACC | 1 cut(s) | 381 |
| EcoRI | GAATTC | 1 cut(s) | 1133 |
| EcoRII | CCWGG | 5 cut(s) | 316, 458, 464, 594, 834 |
| EcoRV | GATATC | 1 cut(s) | 761 |
| EcoT14I | CCWWGG | 3 cut(s) | 109, 286, 1233 |
| EcoT38I | GRGCYC | 2 cut(s) | 1167, 1423 |
| EgeI | GGCGCC | 1 cut(s) | 1300 |
| EheI | GGCGCC | 1 cut(s) | 1300 |
| ErhI | CCWWGG | 3 cut(s) | 109, 286, 1233 |
| FaeI | CATG | 6 cut(s) | 230, 614, 1163, 1199, 1237, 1292 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 37 |
| FaqI | GGGAC | 4 cut(s) | 488, 613, 638, 662 |
| FatI | CATG | 6 cut(s) | 226, 610, 1159, 1195, 1233, 1288 |
| FauNDI | CATATG | 1 cut(s) | 933 |
| Fnu4HI | GCNGC | 2 cut(s) | 100, 555 |
| FokI | GGATG | 5 cut(s) | 420, 718, 902, 950, 1190 |
| FriOI | GRGCYC | 2 cut(s) | 1167, 1423 |
| Fsp4HI | GCNGC | 2 cut(s) | 100, 555 |
| GlaI | GCGC | 4 cut(s) | 43, 261, 1300, 1341 |
| GluI | GCNGC | 2 cut(s) | 100, 555 |
| GsuI | CTGGAG | 1 cut(s) | 932 |
| HaeII | RGCGCY | 4 cut(s) | 45, 263, 1302, 1343 |
| HaeIII | GGCC | 8 cut(s) | 119, 209, 301, 316, 464, 509, 516, 1287 |
| HapII | CCGG | 3 cut(s) | 622, 646, 1253 |
| HhaI | GCGC | 4 cut(s) | 44, 262, 1301, 1342 |
| Hin1I | GRCGYC | 2 cut(s) | 189, 1299 |
| Hin1II | CATG | 6 cut(s) | 230, 614, 1163, 1199, 1237, 1292 |
| Hin6I | GCGC | 4 cut(s) | 42, 260, 1299, 1340 |
| HinP1I | GCGC | 4 cut(s) | 42, 260, 1299, 1340 |
| HincII | GTYRAC | 1 cut(s) | 665 |
| HindII | GTYRAC | 1 cut(s) | 665 |
| HindIII | AAGCTT | 1 cut(s) | 864 |
| HpaII | CCGG | 3 cut(s) | 622, 646, 1253 |
| HphI | GGTGA | 1 cut(s) | 788 |
| Hpy166II | GTNNAC | 3 cut(s) | 543, 665, 1142 |
| Hpy188I | TCNGA | 5 cut(s) | 33, 194, 705, 1105, 1360 |
| Hpy8I | GTNNAC | 3 cut(s) | 543, 665, 1142 |
| Hpy99I | CGWCG | 2 cut(s) | 191, 194 |
| HpyAV | CCTTC | 4 cut(s) | 7, 250, 975, 1291 |
| HpyCH4III | ACNGT | 4 cut(s) | 234, 701, 850, 1457 |
| HpyCH4IV | ACGT | 7 cut(s) | 189, 238, 560, 586, 661, 1017, 1182 |
| HpyCH4V | TGCA | 2 cut(s) | 159, 230 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 48, 206, 268, 563, 872, 893, 902, 1448 |
| HpyF3I | CTNAG | 4 cut(s) | 276, 497, 1311, 1359 |
| HpySE526I | ACGT | 7 cut(s) | 189, 238, 560, 586, 661, 1017, 1182 |
| Hsp92I | GRCGYC | 2 cut(s) | 189, 1299 |
| Hsp92II | CATG | 6 cut(s) | 230, 614, 1163, 1199, 1237, 1292 |
| HspAI | GCGC | 4 cut(s) | 42, 260, 1299, 1340 |
| KasI | GGCGCC | 1 cut(s) | 1298 |
| KflI | GGGWCCC | 2 cut(s) | 625, 649 |
| Kpn2I | TCCGGA | 1 cut(s) | 1252 |
| Kzo9I | GATC | 3 cut(s) | 370, 1146, 1229 |
| LmnI | GCTCC | 3 cut(s) | 739, 951, 1172 |
| Lsp1109I | GCAGC | 2 cut(s) | 86, 566 |
| LweI | GCATC | 1 cut(s) | 353 |
| MaeII | ACGT | 7 cut(s) | 189, 238, 560, 586, 661, 1017, 1182 |
| MaeIII | GTNAC | 5 cut(s) | 234, 381, 710, 1183, 1435 |
| MalI | GATC | 3 cut(s) | 372, 1148, 1231 |
| MboI | GATC | 3 cut(s) | 370, 1146, 1229 |
| MboII | GAAGA | 4 cut(s) | 980, 983, 1225, 1439 |
| MflI | RGATCY | 1 cut(s) | 1229 |
| MhlI | GDGCHC | 3 cut(s) | 1167, 1408, 1423 |
| MlsI | TGGCCA | 2 cut(s) | 209, 316 |
| MluCI | AATT | 6 cut(s) | 722, 1133, 1222, 1261, 1346, 1431 |
| MluNI | TGGCCA | 2 cut(s) | 209, 316 |
| Mly113I | GGCGCC | 1 cut(s) | 1299 |
| MlyI | GAGTC | 5 cut(s) | 204, 415, 548, 595, 1364 |
| MmeI | TCCRAC | 3 cut(s) | 172, 302, 683 |
| MnlI | CCTC | 9 cut(s) | 61, 74, 271, 284, 725, 886, 1073, 1265, 1353 |
| Mox20I | TGGCCA | 2 cut(s) | 209, 316 |
| MroI | TCCGGA | 1 cut(s) | 1252 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1110 |
| MscI | TGGCCA | 2 cut(s) | 209, 316 |
| MseI | TTAA | 4 cut(s) | 171, 1119, 1127, 1349 |
| MslI | CAYNNNNRTG | 6 cut(s) | 609, 936, 1071, 1208, 1293, 1323 |
| Msp20I | TGGCCA | 2 cut(s) | 209, 316 |
| MspI | CCGG | 3 cut(s) | 622, 646, 1253 |
| MspR9I | CCNGG | 9 cut(s) | 318, 460, 466, 596, 622, 623, 646, 647, 836 |
| Mva1269I | GAATGC | 1 cut(s) | 412 |
| MvaI | CCWGG | 5 cut(s) | 318, 460, 466, 596, 836 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 48, 206, 268, 563, 872, 893, 902, 1448 |
| NarI | GGCGCC | 1 cut(s) | 1299 |
| NciI | CCSGG | 4 cut(s) | 622, 623, 646, 647 |
| NcoI | CCATGG | 1 cut(s) | 1233 |
| NdeI | CATATG | 1 cut(s) | 933 |
| NdeII | GATC | 3 cut(s) | 370, 1146, 1229 |
| NheI | GCTAGC | 1 cut(s) | 443 |
| NlaIII | CATG | 6 cut(s) | 230, 614, 1163, 1199, 1237, 1292 |
| NlaIV | GGNNCC | 6 cut(s) | 120, 626, 627, 650, 651, 1300 |
| NmeAIII | GCCGAG | 1 cut(s) | 34 |
| NmuCI | GTSAC | 3 cut(s) | 234, 1183, 1435 |
| OliI | CACNNNNGTG | 1 cut(s) | 1071 |
| PagI | TCATGA | 1 cut(s) | 1159 |
| PctI | GAATGC | 1 cut(s) | 412 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1110 |
| PfeI | GAWTC | 5 cut(s) | 807, 820, 941, 1025, 1256 |
| PflFI | GACNNNGTC | 2 cut(s) | 188, 1016 |
| PkrI | GCNGC | 2 cut(s) | 101, 556 |
| PleI | GAGTC | 5 cut(s) | 203, 415, 547, 595, 1363 |
| PluTI | GGCGCC | 1 cut(s) | 1302 |
| PpsI | GAGTC | 5 cut(s) | 203, 415, 547, 595, 1363 |
| PpuMI | RGGWCCY | 2 cut(s) | 625, 649 |
| PshBI | ATTAAT | 1 cut(s) | 1349 |
| PsiI | TTATAA | 1 cut(s) | 1266 |
| Psp124BI | GAGCTC | 2 cut(s) | 1167, 1423 |
| Psp5II | RGGWCCY | 2 cut(s) | 625, 649 |
| Psp6I | CCWGG | 5 cut(s) | 316, 458, 464, 594, 834 |
| PspEI | GGTNACC | 1 cut(s) | 381 |
| PspGI | CCWGG | 5 cut(s) | 316, 458, 464, 594, 834 |
| PspN4I | GGNNCC | 6 cut(s) | 120, 626, 627, 650, 651, 1300 |
| PspPI | GGNCC | 7 cut(s) | 118, 299, 462, 507, 515, 625, 649 |
| PspPPI | RGGWCCY | 2 cut(s) | 625, 649 |
| PstNI | CAGNNNCTG | 1 cut(s) | 595 |
| PsuI | RGATCY | 1 cut(s) | 1229 |
| PsyI | GACNNNGTC | 2 cut(s) | 188, 1016 |
| RsaI | GTAC | 1 cut(s) | 953 |
| RsaNI | GTAC | 1 cut(s) | 952 |
| RseI | CAYNNNNRTG | 6 cut(s) | 609, 936, 1071, 1208, 1293, 1323 |
| SacI | GAGCTC | 2 cut(s) | 1167, 1423 |
| SaqAI | TTAA | 4 cut(s) | 171, 1119, 1127, 1349 |
| SatI | GCNGC | 2 cut(s) | 100, 555 |
| Sau3AI | GATC | 3 cut(s) | 370, 1146, 1229 |
| Sau96I | GGNCC | 7 cut(s) | 118, 299, 462, 507, 515, 625, 649 |
| SchI | GAGTC | 5 cut(s) | 204, 415, 548, 595, 1364 |
| ScrFI | CCNGG | 9 cut(s) | 318, 460, 466, 596, 622, 623, 646, 647, 836 |
| SduI | GDGCHC | 3 cut(s) | 1167, 1408, 1423 |
| SfaNI | GCATC | 1 cut(s) | 353 |
| SfcI | CTRYAG | 1 cut(s) | 352 |
| SfoI | GGCGCC | 1 cut(s) | 1300 |
| SinI | GGWCC | 2 cut(s) | 625, 649 |
| SmaI | CCCGGG | 2 cut(s) | 623, 647 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 609, 936, 1071, 1208, 1293, 1323 |
| SmlI | CTYRAG | 4 cut(s) | 423, 794, 824, 861 |
| SmoI | CTYRAG | 4 cut(s) | 423, 794, 824, 861 |
| Sse9I | AATT | 6 cut(s) | 722, 1133, 1222, 1261, 1346, 1431 |
| SsiI | CCGC | 3 cut(s) | 294, 784, 1440 |
| SspDI | GGCGCC | 1 cut(s) | 1298 |
| SstI | GAGCTC | 2 cut(s) | 1167, 1423 |
| StyD4I | CCNGG | 9 cut(s) | 316, 458, 464, 594, 620, 621, 644, 645, 834 |
| StyI | CCWWGG | 3 cut(s) | 109, 286, 1233 |
| TaaI | ACNGT | 4 cut(s) | 234, 701, 850, 1457 |
| TaiI | ACGT | 7 cut(s) | 192, 241, 563, 589, 664, 1020, 1185 |
| TaqI | TCGA | 1 cut(s) | 1381 |
| TaqII | GACCGA | 2 cut(s) | 679, 815 |
| TasI | AATT | 6 cut(s) | 722, 1133, 1222, 1261, 1346, 1431 |
| TfiI | GAWTC | 5 cut(s) | 807, 820, 941, 1025, 1256 |
| Tru1I | TTAA | 4 cut(s) | 171, 1119, 1127, 1349 |
| Tru9I | TTAA | 4 cut(s) | 171, 1119, 1127, 1349 |
| TscAI | CASTG | 5 cut(s) | 237, 399, 660, 1035, 1192 |
| TseFI | GTSAC | 3 cut(s) | 234, 1183, 1435 |
| TseI | GCWGC | 2 cut(s) | 99, 554 |
| Tsp45I | GTSAC | 3 cut(s) | 234, 1183, 1435 |
| TspDTI | ATGAA | 5 cut(s) | 422, 977, 981, 1148, 1193 |
| TspGWI | ACGGA | 1 cut(s) | 1159 |
| TspMI | CCCGGG | 2 cut(s) | 621, 645 |
| TspRI | CASTG | 5 cut(s) | 237, 399, 660, 1035, 1192 |
| Tth111I | GACNNNGTC | 2 cut(s) | 188, 1016 |
| VpaK11BI | GGWCC | 2 cut(s) | 625, 649 |
| VspI | ATTAAT | 1 cut(s) | 1349 |
| XapI | RAATTY | 1 cut(s) | 1133 |
| XbaI | TCTAGA | 1 cut(s) | 367 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 293 |
| XmaI | CCCGGG | 2 cut(s) | 621, 645 |
| XmaJI | CCTAGG | 1 cut(s) | 109 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1110 |
| ZraI | GACGTC | 1 cut(s) | 190 |