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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 142, 218 |
| Acc36I | ACCTGC | 4 cut(s) | 63, 116, 142, 218 |
| AccB1I | GGYRCC | 2 cut(s) | 1130, 1211 |
| AccBSI | CCGCTC | 1 cut(s) | 127 |
| AccII | CGCG | 1 cut(s) | 1014 |
| AciI | CCGC | 7 cut(s) | 125, 200, 233, 706, 1098, 1134, 1420 |
| AclWI | GGATC | 6 cut(s) | 67, 159, 896, 966, 1368, 1588 |
| AcoI | YGGCCR | 1 cut(s) | 291 |
| AcsI | RAATTY | 3 cut(s) | 761, 773, 1279 |
| AcyI | GRCGYC | 1 cut(s) | 1212 |
| AfaI | GTAC | 2 cut(s) | 106, 593 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 296, 1023, 1024, 1025, 1156, 1432, 1465 |
| AflIII | ACRYGT | 1 cut(s) | 480 |
| AgsI | TTSAA | 5 cut(s) | 182, 721, 1057, 1247, 1586 |
| AjiI | CACGTC | 1 cut(s) | 481 |
| AjnI | CCWGG | 3 cut(s) | 450, 474, 1216 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 633, 665 |
| AluBI | AGCT | 9 cut(s) | 97, 187, 230, 241, 282, 287, 500, 907, 917 |
| AluI | AGCT | 9 cut(s) | 97, 187, 230, 241, 282, 287, 500, 907, 917 |
| Alw21I | GWGCWC | 2 cut(s) | 99, 909 |
| Alw26I | GTCTC | 4 cut(s) | 45, 570, 637, 841 |
| AlwI | GGATC | 6 cut(s) | 67, 159, 896, 966, 1368, 1588 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 24, 1264 |
| Ama87I | CYCGRG | 1 cut(s) | 1463 |
| AoxI | GGCC | 4 cut(s) | 291, 438, 1030, 1268 |
| ApeKI | GCWGC | 4 cut(s) | 187, 227, 269, 1226 |
| ApoI | RAATTY | 3 cut(s) | 761, 773, 1279 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1464, 1496 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 750 |
| AspLEI | GCGC | 3 cut(s) | 1016, 1166, 1214 |
| AspS9I | GGNCC | 4 cut(s) | 1030, 1066, 1444, 1480 |
| AsuHPI | GGTGA | 5 cut(s) | 111, 311, 1217, 1225, 1232 |
| AsuNHI | GCTAGC | 1 cut(s) | 1160 |
| AvaI | CYCGRG | 1 cut(s) | 1463 |
| AvaII | GGWCC | 3 cut(s) | 1066, 1444, 1480 |
| AxyI | CCTNAGG | 1 cut(s) | 1042 |
| BaeGI | GKGCMC | 1 cut(s) | 1133 |
| BanI | GGYRCC | 2 cut(s) | 1130, 1211 |
| BanII | GRGCYC | 3 cut(s) | 99, 909, 1041 |
| BbsI | GAAGAC | 2 cut(s) | 618, 821 |
| Bbv12I | GWGCWC | 2 cut(s) | 99, 909 |
| BbvCI | CCTCAGC | 1 cut(s) | 1265 |
| BbvI | GCAGC | 4 cut(s) | 174, 239, 281, 1213 |
| BccI | CCATC | 9 cut(s) | 265, 302, 437, 560, 687, 879, 1202, 1206, 1467 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 17, 51, 1383, 1417 |
| BciT130I | CCWGG | 3 cut(s) | 452, 476, 1218 |
| BcoDI | GTCTC | 4 cut(s) | 45, 570, 637, 841 |
| BfaI | CTAG | 4 cut(s) | 204, 471, 683, 1161 |
| BfmI | CTRYAG | 2 cut(s) | 188, 267 |
| BfoI | RGCGCY | 1 cut(s) | 1215 |
| BfuAI | ACCTGC | 4 cut(s) | 63, 116, 142, 218 |
| BglII | AGATCT | 1 cut(s) | 8 |
| BisI | GCNGC | 4 cut(s) | 188, 228, 270, 1227 |
| BlpI | GCTNAGC | 1 cut(s) | 283 |
| BlsI | GCNGC | 4 cut(s) | 189, 229, 271, 1228 |
| Bme1390I | CCNGG | 3 cut(s) | 452, 476, 1218 |
| Bme18I | GGWCC | 3 cut(s) | 1066, 1444, 1480 |
| BmeT110I | CYCGRG | 1 cut(s) | 1463 |
| BmgBI | CACGTC | 1 cut(s) | 481 |
| BmgT120I | GGNCC | 4 cut(s) | 1030, 1066, 1444, 1480 |
| BmiI | GGNNCC | 5 cut(s) | 1031, 1120, 1132, 1213, 1424 |
| BmrFI | CCNGG | 3 cut(s) | 452, 476, 1218 |
| BmsI | GCATC | 5 cut(s) | 151, 439, 1203, 1213, 1403 |
| BmtI | GCTAGC | 1 cut(s) | 1164 |
| BoxI | GACNNNNGTC | 1 cut(s) | 306 |
| BpiI | GAAGAC | 2 cut(s) | 618, 821 |
| BpmI | CTGGAG | 1 cut(s) | 572 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1265 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 283 |
| BpuEI | CTTGAG | 2 cut(s) | 419, 449 |
| BsaHI | GRCGYC | 1 cut(s) | 1212 |
| BsaI | GGTCTC | 2 cut(s) | 45, 637 |
| BsaJI | CCNNGG | 3 cut(s) | 975, 1154, 1216 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 296, 1023, 1024, 1025, 1156, 1432, 1465 |
| Bse1I | ACTGG | 2 cut(s) | 394, 589 |
| Bse21I | CCTNAGG | 1 cut(s) | 1042 |
| Bse3DI | GCAATG | 2 cut(s) | 807, 1415 |
| BseBI | CCWGG | 3 cut(s) | 452, 476, 1218 |
| BseDI | CCNNGG | 3 cut(s) | 975, 1154, 1216 |
| BseGI | GGATG | 3 cut(s) | 555, 583, 1194 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 296, 1023, 1024, 1025, 1156, 1432, 1465 |
| BseMI | GCAATG | 2 cut(s) | 807, 1415 |
| BseMII | CTCAG | 4 cut(s) | 74, 297, 1256, 1326 |
| BseNI | ACTGG | 2 cut(s) | 394, 589 |
| BseSI | GKGCMC | 1 cut(s) | 1133 |
| BseXI | GCAGC | 4 cut(s) | 174, 239, 281, 1213 |
| BsgI | GTGCAG | 1 cut(s) | 156 |
| Bsh1236I | CGCG | 1 cut(s) | 1014 |
| BshFI | GGCC | 4 cut(s) | 293, 440, 1032, 1270 |
| BshNI | GGYRCC | 2 cut(s) | 1130, 1211 |
| BsiHKAI | GWGCWC | 2 cut(s) | 99, 909 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1463 |
| BsiSI | CCGG | 3 cut(s) | 290, 327, 337 |
| BslFI | GGGAC | 1 cut(s) | 1481 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 296, 1023, 1024, 1025, 1156, 1432, 1465 |
| BsmAI | GTCTC | 4 cut(s) | 45, 570, 637, 841 |
| BsmBI | CGTCTC | 1 cut(s) | 841 |
| BsmFI | GGGAC | 1 cut(s) | 1481 |
| BsnI | GGCC | 4 cut(s) | 293, 440, 1032, 1270 |
| Bso31I | GGTCTC | 2 cut(s) | 45, 637 |
| BsoBI | CYCGRG | 1 cut(s) | 1463 |
| Bsp1286I | GDGCHC | 4 cut(s) | 99, 909, 1041, 1133 |
| Bsp143I | GATC | 7 cut(s) | 8, 72, 164, 888, 971, 1373, 1593 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 283 |
| BspACI | CCGC | 7 cut(s) | 125, 200, 233, 706, 1098, 1134, 1420 |
| BspANI | GGCC | 4 cut(s) | 293, 440, 1032, 1270 |
| BspCNI | CTCAG | 4 cut(s) | 75, 296, 1257, 1327 |
| BspFNI | CGCG | 1 cut(s) | 1014 |
| BspHI | TCATGA | 1 cut(s) | 901 |
| BspLI | GGNNCC | 5 cut(s) | 1031, 1120, 1132, 1213, 1424 |
| BspMAI | CTGCAG | 2 cut(s) | 192, 271 |
| BspMI | ACCTGC | 4 cut(s) | 63, 116, 142, 218 |
| BspOI | GCTAGC | 1 cut(s) | 1164 |
| BspPI | GGATC | 6 cut(s) | 67, 159, 896, 966, 1368, 1588 |
| BspT107I | GGYRCC | 2 cut(s) | 1130, 1211 |
| BspTNI | GGTCTC | 2 cut(s) | 45, 637 |
| BsrBI | CCGCTC | 1 cut(s) | 127 |
| BsrDI | GCAATG | 2 cut(s) | 807, 1415 |
| BsrI | ACTGG | 2 cut(s) | 394, 589 |
| BssECI | CCNNGG | 3 cut(s) | 975, 1154, 1216 |
| BssMI | GATC | 7 cut(s) | 8, 72, 164, 888, 971, 1373, 1593 |
| BssNI | GRCGYC | 1 cut(s) | 1212 |
| BssT1I | CCWWGG | 1 cut(s) | 1154 |
| Bst2UI | CCWGG | 3 cut(s) | 452, 476, 1218 |
| Bst4CI | ACNGT | 3 cut(s) | 308, 490, 1477 |
| Bst6I | CTCTTC | 2 cut(s) | 1119, 1432 |
| BstACI | GRCGYC | 1 cut(s) | 1212 |
| BstC8I | GCNNGC | 4 cut(s) | 175, 267, 498, 1162 |
| BstDEI | CTNAG | 7 cut(s) | 83, 283, 529, 855, 1042, 1265, 1335 |
| BstDSI | CCRYGG | 1 cut(s) | 975 |
| BstEII | GGTNACC | 2 cut(s) | 299, 1093 |
| BstF5I | GGATG | 3 cut(s) | 555, 583, 1194 |
| BstFNI | CGCG | 1 cut(s) | 1014 |
| BstH2I | RGCGCY | 1 cut(s) | 1215 |
| BstHHI | GCGC | 3 cut(s) | 1016, 1166, 1214 |
| BstKTI | GATC | 7 cut(s) | 11, 75, 167, 891, 974, 1376, 1596 |
| BstMAI | GTCTC | 4 cut(s) | 45, 570, 637, 841 |
| BstMBI | GATC | 7 cut(s) | 8, 72, 164, 888, 971, 1373, 1593 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 262, 521, 542, 1300 |
| BstNI | CCWGG | 3 cut(s) | 452, 476, 1218 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 306 |
| BstPI | GGTNACC | 2 cut(s) | 299, 1093 |
| BstSCI | CCNGG | 3 cut(s) | 450, 474, 1216 |
| BstSFI | CTRYAG | 2 cut(s) | 188, 267 |
| BstSLI | GKGCMC | 1 cut(s) | 1133 |
| BstUI | CGCG | 1 cut(s) | 1014 |
| BstV1I | GCAGC | 4 cut(s) | 174, 239, 281, 1213 |
| BstV2I | GAAGAC | 2 cut(s) | 618, 821 |
| BstX2I | RGATCY | 3 cut(s) | 8, 164, 971 |
| BstYI | RGATCY | 3 cut(s) | 8, 164, 971 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1042 |
| BsuRI | GGCC | 4 cut(s) | 293, 440, 1032, 1270 |
| BtgI | CCRYGG | 1 cut(s) | 975 |
| BtgZI | GCGATG | 1 cut(s) | 1386 |
| BtrI | CACGTC | 1 cut(s) | 481 |
| BtsCI | GGATG | 3 cut(s) | 555, 583, 1194 |
| BtsI | GCAGTG | 1 cut(s) | 1173 |
| BtsIMutI | CAGTG | 1 cut(s) | 1173 |
| BveI | ACCTGC | 4 cut(s) | 63, 116, 142, 218 |
| Cac8I | GCNNGC | 4 cut(s) | 175, 267, 498, 1162 |
| CaiI | CAGNNNCTG | 2 cut(s) | 24, 1264 |
| CciI | TCATGA | 1 cut(s) | 901 |
| CfoI | GCGC | 3 cut(s) | 1016, 1166, 1214 |
| Cfr13I | GGNCC | 4 cut(s) | 1030, 1066, 1444, 1480 |
| CseI | GACGC | 1 cut(s) | 1270 |
| Csp6I | GTAC | 2 cut(s) | 105, 592 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1136, 1171 |
| CviAII | CATG | 5 cut(s) | 68, 221, 902, 938, 1202 |
| CviQI | GTAC | 2 cut(s) | 105, 592 |
| DdeI | CTNAG | 7 cut(s) | 83, 283, 529, 855, 1042, 1265, 1335 |
| DinI | GGCGCC | 1 cut(s) | 1213 |
| DpnI | GATC | 7 cut(s) | 10, 74, 166, 890, 973, 1375, 1595 |
| DpnII | GATC | 7 cut(s) | 8, 72, 164, 888, 971, 1373, 1593 |
| EaeI | YGGCCR | 1 cut(s) | 291 |
| Eam1104I | CTCTTC | 2 cut(s) | 1119, 1432 |
| EarI | CTCTTC | 2 cut(s) | 1119, 1432 |
| Ecl136II | GAGCTC | 2 cut(s) | 97, 907 |
| Eco130I | CCWWGG | 1 cut(s) | 1154 |
| Eco147I | AGGCCT | 1 cut(s) | 1270 |
| Eco24I | GRGCYC | 3 cut(s) | 99, 909, 1041 |
| Eco31I | GGTCTC | 2 cut(s) | 45, 637 |
| Eco32I | GATATC | 1 cut(s) | 401 |
| Eco47I | GGWCC | 3 cut(s) | 1066, 1444, 1480 |
| Eco53kI | GAGCTC | 2 cut(s) | 97, 907 |
| Eco81I | CCTNAGG | 1 cut(s) | 1042 |
| Eco88I | CYCGRG | 1 cut(s) | 1463 |
| Eco91I | GGTNACC | 2 cut(s) | 299, 1093 |
| EcoICRI | GAGCTC | 2 cut(s) | 97, 907 |
| EcoO65I | GGTNACC | 2 cut(s) | 299, 1093 |
| EcoRI | GAATTC | 1 cut(s) | 773 |
| EcoRII | CCWGG | 3 cut(s) | 450, 474, 1216 |
| EcoRV | GATATC | 1 cut(s) | 401 |
| EcoT14I | CCWWGG | 1 cut(s) | 1154 |
| EcoT38I | GRGCYC | 3 cut(s) | 99, 909, 1041 |
| EgeI | GGCGCC | 1 cut(s) | 1213 |
| EheI | GGCGCC | 1 cut(s) | 1213 |
| ErhI | CCWWGG | 1 cut(s) | 1154 |
| Esp3I | CGTCTC | 1 cut(s) | 841 |
| FaeI | CATG | 5 cut(s) | 71, 224, 905, 941, 1205 |
| FaqI | GGGAC | 1 cut(s) | 1481 |
| FatI | CATG | 5 cut(s) | 67, 220, 901, 937, 1201 |
| FauI | CCCGC | 2 cut(s) | 132, 207 |
| FauNDI | CATATG | 1 cut(s) | 573 |
| Fnu4HI | GCNGC | 4 cut(s) | 188, 228, 270, 1227 |
| FokI | GGATG | 3 cut(s) | 542, 590, 1181 |
| FriOI | GRGCYC | 3 cut(s) | 99, 909, 1041 |
| Fsp4HI | GCNGC | 4 cut(s) | 188, 228, 270, 1227 |
| FspBI | CTAG | 4 cut(s) | 204, 471, 683, 1161 |
| GlaI | GCGC | 3 cut(s) | 1015, 1165, 1213 |
| GluI | GCNGC | 4 cut(s) | 188, 228, 270, 1227 |
| GsuI | CTGGAG | 1 cut(s) | 572 |
| HaeII | RGCGCY | 1 cut(s) | 1215 |
| HaeIII | GGCC | 4 cut(s) | 293, 440, 1032, 1270 |
| HapII | CCGG | 3 cut(s) | 290, 327, 337 |
| HgaI | GACGC | 1 cut(s) | 1270 |
| HhaI | GCGC | 3 cut(s) | 1016, 1166, 1214 |
| Hin1I | GRCGYC | 1 cut(s) | 1212 |
| Hin1II | CATG | 5 cut(s) | 71, 224, 905, 941, 1205 |
| Hin6I | GCGC | 3 cut(s) | 1014, 1164, 1212 |
| HinP1I | GCGC | 3 cut(s) | 1014, 1164, 1212 |
| HincII | GTYRAC | 2 cut(s) | 150, 486 |
| HindII | GTYRAC | 2 cut(s) | 150, 486 |
| HinfI | GANTC | 8 cut(s) | 41, 85, 447, 460, 581, 665, 817, 1352 |
| HpaI | GTTAAC | 1 cut(s) | 486 |
| HpaII | CCGG | 3 cut(s) | 290, 327, 337 |
| HphI | GGTGA | 5 cut(s) | 111, 311, 1217, 1225, 1232 |
| Hpy166II | GTNNAC | 3 cut(s) | 150, 486, 1144 |
| Hpy188I | TCNGA | 4 cut(s) | 312, 745, 996, 1260 |
| Hpy188III | TCNNGA | 9 cut(s) | 396, 416, 677, 731, 902, 969, 1356, 1434, 1623 |
| Hpy8I | GTNNAC | 3 cut(s) | 150, 486, 1144 |
| HpyAV | CCTTC | 4 cut(s) | 188, 615, 838, 1084 |
| HpyCH4III | ACNGT | 3 cut(s) | 308, 490, 1477 |
| HpyCH4IV | ACGT | 3 cut(s) | 480, 657, 1383 |
| HpyCH4V | TGCA | 9 cut(s) | 173, 190, 213, 246, 269, 800, 842, 1229, 1415 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 262, 521, 542, 1300 |
| HpyF3I | CTNAG | 7 cut(s) | 83, 283, 529, 855, 1042, 1265, 1335 |
| HpySE526I | ACGT | 3 cut(s) | 480, 657, 1383 |
| Hsp92I | GRCGYC | 1 cut(s) | 1212 |
| Hsp92II | CATG | 5 cut(s) | 71, 224, 905, 941, 1205 |
| HspAI | GCGC | 3 cut(s) | 1014, 1164, 1212 |
| KasI | GGCGCC | 1 cut(s) | 1211 |
| KspAI | GTTAAC | 1 cut(s) | 486 |
| Kzo9I | GATC | 7 cut(s) | 8, 72, 164, 888, 971, 1373, 1593 |
| LmnI | GCTCC | 6 cut(s) | 116, 235, 292, 379, 591, 914 |
| Lsp1109I | GCAGC | 4 cut(s) | 174, 239, 281, 1213 |
| LweI | GCATC | 5 cut(s) | 151, 439, 1203, 1213, 1403 |
| MaeI | CTAG | 4 cut(s) | 204, 471, 683, 1161 |
| MaeII | ACGT | 3 cut(s) | 480, 657, 1383 |
| MaeIII | GTNAC | 8 cut(s) | 299, 350, 662, 874, 925, 1093, 1330, 1353 |
| MalI | GATC | 7 cut(s) | 10, 74, 166, 890, 973, 1375, 1595 |
| MbiI | CCGCTC | 1 cut(s) | 127 |
| MboI | GATC | 7 cut(s) | 8, 72, 164, 888, 971, 1373, 1593 |
| MboII | GAAGA | 9 cut(s) | 252, 620, 623, 653, 826, 967, 1136, 1389, 1449 |
| MflI | RGATCY | 3 cut(s) | 8, 164, 971 |
| MhlI | GDGCHC | 4 cut(s) | 99, 909, 1041, 1133 |
| MluCI | AATT | 8 cut(s) | 362, 761, 773, 867, 964, 1003, 1279, 1451 |
| Mly113I | GGCGCC | 1 cut(s) | 1212 |
| MlyI | GAGTC | 5 cut(s) | 50, 94, 659, 811, 1361 |
| MroXI | GAANNNNTTC | 1 cut(s) | 750 |
| MseI | TTAA | 5 cut(s) | 485, 759, 767, 1322, 1559 |
| MslI | CAYNNNNRTG | 6 cut(s) | 560, 576, 950, 1056, 1203, 1206 |
| MspA1I | CMGCKG | 1 cut(s) | 187 |
| MspI | CCGG | 3 cut(s) | 290, 327, 337 |
| MspR9I | CCNGG | 3 cut(s) | 452, 476, 1218 |
| MvaI | CCWGG | 3 cut(s) | 452, 476, 1218 |
| MvnI | CGCG | 1 cut(s) | 1014 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 262, 521, 542, 1300 |
| NarI | GGCGCC | 1 cut(s) | 1212 |
| NdeI | CATATG | 1 cut(s) | 573 |
| NdeII | GATC | 7 cut(s) | 8, 72, 164, 888, 971, 1373, 1593 |
| NheI | GCTAGC | 1 cut(s) | 1160 |
| NlaIII | CATG | 5 cut(s) | 71, 224, 905, 941, 1205 |
| NlaIV | GGNNCC | 5 cut(s) | 1031, 1120, 1132, 1213, 1424 |
| NmeAIII | GCCGAG | 3 cut(s) | 64, 118, 163 |
| NmuCI | GTSAC | 5 cut(s) | 299, 662, 925, 1330, 1353 |
| PagI | TCATGA | 1 cut(s) | 901 |
| PaqCI | CACCTGC | 2 cut(s) | 142, 218 |
| PceI | AGGCCT | 1 cut(s) | 1270 |
| PdmI | GAANNNNTTC | 1 cut(s) | 750 |
| PfeI | GAWTC | 3 cut(s) | 447, 460, 581 |
| PfoI | TCCNGGA | 1 cut(s) | 450 |
| PkrI | GCNGC | 4 cut(s) | 189, 229, 271, 1228 |
| PleI | GAGTC | 5 cut(s) | 49, 93, 659, 811, 1360 |
| PluTI | GGCGCC | 1 cut(s) | 1215 |
| PpsI | GAGTC | 5 cut(s) | 49, 93, 659, 811, 1360 |
| PshAI | GACNNNNGTC | 1 cut(s) | 306 |
| Psp124BI | GAGCTC | 2 cut(s) | 99, 909 |
| Psp6I | CCWGG | 3 cut(s) | 450, 474, 1216 |
| PspEI | GGTNACC | 2 cut(s) | 299, 1093 |
| PspGI | CCWGG | 3 cut(s) | 450, 474, 1216 |
| PspN4I | GGNNCC | 5 cut(s) | 1031, 1120, 1132, 1213, 1424 |
| PspPI | GGNCC | 4 cut(s) | 1030, 1066, 1444, 1480 |
| PstI | CTGCAG | 2 cut(s) | 192, 271 |
| PstNI | CAGNNNCTG | 2 cut(s) | 24, 1264 |
| PsuI | RGATCY | 3 cut(s) | 8, 164, 971 |
| PvuII | CAGCTG | 1 cut(s) | 187 |
| RsaI | GTAC | 2 cut(s) | 106, 593 |
| RsaNI | GTAC | 2 cut(s) | 105, 592 |
| RseI | CAYNNNNRTG | 6 cut(s) | 560, 576, 950, 1056, 1203, 1206 |
| SacI | GAGCTC | 2 cut(s) | 99, 909 |
| SaqAI | TTAA | 5 cut(s) | 485, 759, 767, 1322, 1559 |
| SatI | GCNGC | 4 cut(s) | 188, 228, 270, 1227 |
| Sau3AI | GATC | 7 cut(s) | 8, 72, 164, 888, 971, 1373, 1593 |
| Sau96I | GGNCC | 4 cut(s) | 1030, 1066, 1444, 1480 |
| SchI | GAGTC | 5 cut(s) | 50, 94, 659, 811, 1361 |
| ScrFI | CCNGG | 3 cut(s) | 452, 476, 1218 |
| SduI | GDGCHC | 4 cut(s) | 99, 909, 1041, 1133 |
| SfaNI | GCATC | 5 cut(s) | 151, 439, 1203, 1213, 1403 |
| SfcI | CTRYAG | 2 cut(s) | 188, 267 |
| SfoI | GGCGCC | 1 cut(s) | 1213 |
| SinI | GGWCC | 3 cut(s) | 1066, 1444, 1480 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 560, 576, 950, 1056, 1203, 1206 |
| SmlI | CTYRAG | 2 cut(s) | 434, 464 |
| SmoI | CTYRAG | 2 cut(s) | 434, 464 |
| Sse9I | AATT | 8 cut(s) | 362, 761, 773, 867, 964, 1003, 1279, 1451 |
| SseBI | AGGCCT | 1 cut(s) | 1270 |
| SsiI | CCGC | 7 cut(s) | 125, 200, 233, 706, 1098, 1134, 1420 |
| SspDI | GGCGCC | 1 cut(s) | 1211 |
| SspMI | CTAG | 4 cut(s) | 204, 471, 683, 1161 |
| SstI | GAGCTC | 2 cut(s) | 99, 909 |
| StuI | AGGCCT | 1 cut(s) | 1270 |
| StyD4I | CCNGG | 3 cut(s) | 450, 474, 1216 |
| StyI | CCWWGG | 1 cut(s) | 1154 |
| TaaI | ACNGT | 3 cut(s) | 308, 490, 1477 |
| TaiI | ACGT | 3 cut(s) | 483, 660, 1386 |
| TaqI | TCGA | 3 cut(s) | 639, 865, 968 |
| TasI | AATT | 8 cut(s) | 362, 761, 773, 867, 964, 1003, 1279, 1451 |
| TfiI | GAWTC | 3 cut(s) | 447, 460, 581 |
| Tru1I | TTAA | 5 cut(s) | 485, 759, 767, 1322, 1559 |
| Tru9I | TTAA | 5 cut(s) | 485, 759, 767, 1322, 1559 |
| TscAI | CASTG | 1 cut(s) | 1173 |
| TseFI | GTSAC | 5 cut(s) | 299, 662, 925, 1330, 1353 |
| TseI | GCWGC | 4 cut(s) | 187, 227, 269, 1226 |
| Tsp45I | GTSAC | 5 cut(s) | 299, 662, 925, 1330, 1353 |
| TspDTI | ATGAA | 7 cut(s) | 617, 621, 890, 935, 1389, 1521, 1603 |
| TspGWI | ACGGA | 5 cut(s) | 338, 650, 964, 1129, 1131 |
| TspRI | CASTG | 1 cut(s) | 1173 |
| VpaK11BI | GGWCC | 3 cut(s) | 1066, 1444, 1480 |
| XapI | RAATTY | 3 cut(s) | 761, 773, 1279 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1205 |
| XmnI | GAANNNNTTC | 1 cut(s) | 750 |
| XspI | CTAG | 4 cut(s) | 204, 471, 683, 1161 |