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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 915, 960 |
| AatII | GACGTC | 1 cut(s) | 523 |
| AccB1I | GGYRCC | 3 cut(s) | 451, 1136, 1157 |
| AccBSI | CCGCTC | 1 cut(s) | 144 |
| AccI | GTMKAC | 2 cut(s) | 1180, 1329 |
| AciI | CCGC | 8 cut(s) | 144, 178, 208, 271, 1036, 1057, 1070, 1416 |
| AclWI | GGATC | 4 cut(s) | 61, 471, 848, 1246 |
| AcoI | YGGCCR | 1 cut(s) | 1123 |
| AcuI | CTGAAG | 2 cut(s) | 102, 1413 |
| AcvI | CACGTG | 3 cut(s) | 323, 1079, 1097 |
| AcyI | GRCGYC | 2 cut(s) | 520, 1137 |
| AdeI | CACNNNGTG | 1 cut(s) | 1082 |
| AfaI | GTAC | 3 cut(s) | 102, 477, 820 |
| AfeI | AGCGCT | 2 cut(s) | 588, 599 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 394, 974, 1045, 1098, 1127 |
| AflIII | ACRYGT | 3 cut(s) | 322, 1076, 1094 |
| AgsI | TTSAA | 8 cut(s) | 426, 637, 644, 673, 921, 1201, 1258, 1387 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 673, 740 |
| AhlI | ACTAGT | 1 cut(s) | 257 |
| AjnI | CCWGG | 3 cut(s) | 447, 1044, 1120 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 585, 617 |
| AleI | CACNNNNGTG | 2 cut(s) | 96, 321 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 455, 487 |
| AluBI | AGCT | 9 cut(s) | 76, 80, 577, 701, 757, 859, 881, 1286, 1391 |
| AluI | AGCT | 9 cut(s) | 76, 80, 577, 701, 757, 859, 881, 1286, 1391 |
| Alw21I | GWGCWC | 3 cut(s) | 1192, 1251, 1407 |
| Alw26I | GTCTC | 2 cut(s) | 200, 511 |
| Alw44I | GTGCAC | 2 cut(s) | 1188, 1403 |
| AlwI | GGATC | 4 cut(s) | 61, 471, 848, 1246 |
| Ama87I | CYCGRG | 1 cut(s) | 139 |
| Aor51HI | AGCGCT | 2 cut(s) | 588, 599 |
| AoxI | GGCC | 4 cut(s) | 145, 923, 1024, 1123 |
| ApaI | GGGCCC | 1 cut(s) | 1028 |
| ApaLI | GTGCAC | 2 cut(s) | 1188, 1403 |
| ApeKI | GCWGC | 9 cut(s) | 203, 228, 245, 248, 251, 264, 273, 286, 1286 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 702 |
| AspA2I | CCTAGG | 1 cut(s) | 1169 |
| AspLEI | GCGC | 4 cut(s) | 589, 600, 660, 1139 |
| AspS9I | GGNCC | 4 cut(s) | 146, 924, 1024, 1025 |
| AsuC2I | CCSGG | 1 cut(s) | 461 |
| AsuHPI | GGTGA | 1 cut(s) | 1363 |
| AsuNHI | GCTAGC | 1 cut(s) | 76 |
| AvaI | CYCGRG | 1 cut(s) | 139 |
| AvrII | CCTAGG | 1 cut(s) | 1169 |
| BaeGI | GKGCMC | 3 cut(s) | 1028, 1192, 1407 |
| BalI | TGGCCA | 1 cut(s) | 1125 |
| BanI | GGYRCC | 3 cut(s) | 451, 1136, 1157 |
| BanII | GRGCYC | 1 cut(s) | 1028 |
| BauI | CACGAG | 1 cut(s) | 844 |
| BbrPI | CACGTG | 3 cut(s) | 323, 1079, 1097 |
| Bbv12I | GWGCWC | 3 cut(s) | 1192, 1251, 1407 |
| BbvI | GCAGC | 9 cut(s) | 215, 232, 235, 238, 240, 251, 260, 273, 1273 |
| BccI | CCATC | 1 cut(s) | 423 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 116, 150 |
| BciT130I | CCWGG | 3 cut(s) | 449, 1046, 1122 |
| BciVI | GTATCC | 1 cut(s) | 1066 |
| BcnI | CCSGG | 1 cut(s) | 461 |
| BcoDI | GTCTC | 2 cut(s) | 200, 511 |
| BcuI | ACTAGT | 1 cut(s) | 257 |
| BfaI | CTAG | 7 cut(s) | 77, 174, 236, 258, 590, 1170, 1283 |
| BfmI | CTRYAG | 6 cut(s) | 57, 165, 201, 226, 287, 876 |
| BfoI | RGCGCY | 3 cut(s) | 590, 601, 1140 |
| BfuI | GTATCC | 1 cut(s) | 1066 |
| BlnI | CCTAGG | 1 cut(s) | 1169 |
| Bme1390I | CCNGG | 4 cut(s) | 449, 461, 1046, 1122 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 673, 740 |
| BmeT110I | CYCGRG | 1 cut(s) | 139 |
| BmgT120I | GGNCC | 4 cut(s) | 146, 924, 1024, 1025 |
| BmiI | GGNNCC | 4 cut(s) | 453, 1026, 1138, 1159 |
| BmrFI | CCNGG | 4 cut(s) | 449, 461, 1046, 1122 |
| BmrI | ACTGGG | 2 cut(s) | 403, 779 |
| BmsI | GCATC | 3 cut(s) | 556, 1057, 1402 |
| BmtI | GCTAGC | 1 cut(s) | 80 |
| BmuI | ACTGGG | 2 cut(s) | 403, 779 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 133, 165 |
| BpmI | CTGGAG | 1 cut(s) | 200 |
| BpuEI | CTTGAG | 3 cut(s) | 314, 483, 1333 |
| BpuMI | CCSGG | 1 cut(s) | 461 |
| BsaAI | YACGTR | 3 cut(s) | 323, 1079, 1097 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1365 |
| BsaHI | GRCGYC | 2 cut(s) | 520, 1137 |
| BsaJI | CCNNGG | 3 cut(s) | 1120, 1126, 1169 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 294, 324, 1093, 1123 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 394, 974, 1045, 1098, 1127 |
| Bse1I | ACTGG | 3 cut(s) | 398, 785, 1256 |
| Bse3DI | GCAATG | 2 cut(s) | 613, 1268 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1365 |
| BseBI | CCWGG | 3 cut(s) | 449, 1046, 1122 |
| BseDI | CCNNGG | 3 cut(s) | 1120, 1126, 1169 |
| BseGI | GGATG | 1 cut(s) | 1417 |
| BseJI | GATNNNNATC | 1 cut(s) | 1365 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 394, 974, 1045, 1098, 1127 |
| BseMI | GCAATG | 2 cut(s) | 613, 1268 |
| BseMII | CTCAG | 2 cut(s) | 553, 873 |
| BseNI | ACTGG | 3 cut(s) | 398, 785, 1256 |
| BseRI | GAGGAG | 1 cut(s) | 614 |
| BseSI | GKGCMC | 3 cut(s) | 1028, 1192, 1407 |
| BseXI | GCAGC | 9 cut(s) | 215, 232, 235, 238, 240, 251, 260, 273, 1273 |
| BsgI | GTGCAG | 1 cut(s) | 250 |
| BshFI | GGCC | 4 cut(s) | 147, 925, 1026, 1125 |
| BshNI | GGYRCC | 3 cut(s) | 451, 1136, 1157 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1192, 1251, 1407 |
| BsiHKCI | CYCGRG | 1 cut(s) | 139 |
| BsiSI | CCGG | 1 cut(s) | 461 |
| BsiWI | CGTACG | 1 cut(s) | 475 |
| BslFI | GGGAC | 4 cut(s) | 155, 1020, 1029, 1322 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 394, 974, 1045, 1098, 1127 |
| BsmAI | GTCTC | 2 cut(s) | 200, 511 |
| BsmBI | CGTCTC | 1 cut(s) | 511 |
| BsmFI | GGGAC | 4 cut(s) | 155, 1020, 1029, 1322 |
| BsnI | GGCC | 4 cut(s) | 147, 925, 1026, 1125 |
| BsoBI | CYCGRG | 1 cut(s) | 139 |
| Bsp120I | GGGCCC | 1 cut(s) | 1024 |
| Bsp1286I | GDGCHC | 4 cut(s) | 1028, 1192, 1251, 1407 |
| Bsp143I | GATC | 5 cut(s) | 66, 463, 558, 840, 1238 |
| Bsp19I | CCATGG | 1 cut(s) | 1126 |
| BspACI | CCGC | 8 cut(s) | 144, 178, 208, 271, 1036, 1057, 1070, 1416 |
| BspANI | GGCC | 4 cut(s) | 147, 925, 1026, 1125 |
| BspCNI | CTCAG | 2 cut(s) | 552, 874 |
| BspHI | TCATGA | 2 cut(s) | 903, 946 |
| BspLI | GGNNCC | 4 cut(s) | 453, 1026, 1138, 1159 |
| BspMAI | CTGCAG | 4 cut(s) | 169, 205, 230, 291 |
| BspOI | GCTAGC | 1 cut(s) | 80 |
| BspPI | GGATC | 4 cut(s) | 61, 471, 848, 1246 |
| BspQI | GCTCTTC | 1 cut(s) | 708 |
| BspT107I | GGYRCC | 3 cut(s) | 451, 1136, 1157 |
| BsrBI | CCGCTC | 1 cut(s) | 144 |
| BsrDI | GCAATG | 2 cut(s) | 613, 1268 |
| BsrI | ACTGG | 3 cut(s) | 398, 785, 1256 |
| BssECI | CCNNGG | 3 cut(s) | 1120, 1126, 1169 |
| BssMI | GATC | 5 cut(s) | 66, 463, 558, 840, 1238 |
| BssNAI | GTATAC | 1 cut(s) | 1330 |
| BssNI | GRCGYC | 2 cut(s) | 520, 1137 |
| BssSI | CACGAG | 1 cut(s) | 844 |
| BssT1I | CCWWGG | 2 cut(s) | 1126, 1169 |
| Bst1107I | GTATAC | 1 cut(s) | 1330 |
| Bst2BI | CACGAG | 1 cut(s) | 844 |
| Bst2UI | CCWGG | 3 cut(s) | 449, 1046, 1122 |
| Bst4CI | ACNGT | 4 cut(s) | 37, 98, 105, 188 |
| Bst6I | CTCTTC | 1 cut(s) | 708 |
| BstACI | GRCGYC | 2 cut(s) | 520, 1137 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 273 |
| BstBAI | YACGTR | 3 cut(s) | 323, 1079, 1097 |
| BstC8I | GCNNGC | 6 cut(s) | 78, 208, 308, 495, 1268, 1277 |
| BstDEI | CTNAG | 4 cut(s) | 539, 882, 936, 996 |
| BstDSI | CCRYGG | 1 cut(s) | 1126 |
| BstEII | GGTNACC | 1 cut(s) | 397 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 972 |
| BstF5I | GGATG | 1 cut(s) | 1417 |
| BstH2I | RGCGCY | 3 cut(s) | 590, 601, 1140 |
| BstHHI | GCGC | 4 cut(s) | 589, 600, 660, 1139 |
| BstKTI | GATC | 5 cut(s) | 69, 466, 561, 843, 1241 |
| BstMAI | GTCTC | 2 cut(s) | 200, 511 |
| BstMBI | GATC | 5 cut(s) | 66, 463, 558, 840, 1238 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 17, 251, 270, 273, 595, 1267, 1272, 1276 |
| BstNI | CCWGG | 3 cut(s) | 449, 1046, 1122 |
| BstNSI | RCATGY | 2 cut(s) | 378, 497 |
| BstPI | GGTNACC | 1 cut(s) | 397 |
| BstSCI | CCNGG | 4 cut(s) | 447, 459, 1044, 1120 |
| BstSFI | CTRYAG | 6 cut(s) | 57, 165, 201, 226, 287, 876 |
| BstSLI | GKGCMC | 3 cut(s) | 1028, 1192, 1407 |
| BstV1I | GCAGC | 9 cut(s) | 215, 232, 235, 238, 240, 251, 260, 273, 1273 |
| BstX2I | RGATCY | 1 cut(s) | 463 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1133 |
| BstYI | RGATCY | 1 cut(s) | 463 |
| BstZ17I | GTATAC | 1 cut(s) | 1330 |
| BsuI | GTATCC | 1 cut(s) | 1066 |
| BsuRI | GGCC | 4 cut(s) | 147, 925, 1026, 1125 |
| BtgI | CCRYGG | 1 cut(s) | 1126 |
| BtsCI | GGATG | 1 cut(s) | 1417 |
| BtsIMutI | CAGTG | 1 cut(s) | 1249 |
| Cac8I | GCNNGC | 6 cut(s) | 78, 208, 308, 495, 1268, 1277 |
| CciI | TCATGA | 2 cut(s) | 903, 946 |
| CfoI | GCGC | 4 cut(s) | 589, 600, 660, 1139 |
| Cfr13I | GGNCC | 4 cut(s) | 146, 924, 1024, 1025 |
| CseI | GACGC | 1 cut(s) | 1214 |
| Csp6I | GTAC | 3 cut(s) | 101, 476, 819 |
| CviQI | GTAC | 3 cut(s) | 101, 476, 819 |
| DdeI | CTNAG | 4 cut(s) | 539, 882, 936, 996 |
| DinI | GGCGCC | 1 cut(s) | 1138 |
| DpnI | GATC | 5 cut(s) | 68, 465, 560, 842, 1240 |
| DpnII | GATC | 5 cut(s) | 66, 463, 558, 840, 1238 |
| DraIII | CACNNNGTG | 1 cut(s) | 1082 |
| DriI | GACNNNNNGTC | 2 cut(s) | 673, 740 |
| EaeI | YGGCCR | 1 cut(s) | 1123 |
| Eam1104I | CTCTTC | 1 cut(s) | 708 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 673, 740 |
| EarI | CTCTTC | 1 cut(s) | 708 |
| Eco130I | CCWWGG | 2 cut(s) | 1126, 1169 |
| Eco24I | GRGCYC | 1 cut(s) | 1028 |
| Eco47III | AGCGCT | 2 cut(s) | 588, 599 |
| Eco57I | CTGAAG | 2 cut(s) | 102, 1413 |
| Eco72I | CACGTG | 3 cut(s) | 323, 1079, 1097 |
| Eco88I | CYCGRG | 1 cut(s) | 139 |
| Eco91I | GGTNACC | 1 cut(s) | 397 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 972 |
| EcoO65I | GGTNACC | 1 cut(s) | 397 |
| EcoRII | CCWGG | 3 cut(s) | 447, 1044, 1120 |
| EcoT14I | CCWWGG | 2 cut(s) | 1126, 1169 |
| EcoT38I | GRGCYC | 1 cut(s) | 1028 |
| EgeI | GGCGCC | 1 cut(s) | 1138 |
| EheI | GGCGCC | 1 cut(s) | 1138 |
| ErhI | CCWWGG | 2 cut(s) | 1126, 1169 |
| Esp3I | CGTCTC | 1 cut(s) | 511 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1317, 1349 |
| FaqI | GGGAC | 4 cut(s) | 155, 1020, 1029, 1322 |
| FauI | CCCGC | 2 cut(s) | 215, 278 |
| FauNDI | CATATG | 1 cut(s) | 525 |
| FblI | GTMKAC | 2 cut(s) | 1180, 1329 |
| FokI | GGATG | 1 cut(s) | 1424 |
| FriOI | GRGCYC | 1 cut(s) | 1028 |
| FspBI | CTAG | 7 cut(s) | 77, 174, 236, 258, 590, 1170, 1283 |
| GlaI | GCGC | 4 cut(s) | 588, 599, 659, 1138 |
| GsuI | CTGGAG | 1 cut(s) | 200 |
| HaeII | RGCGCY | 3 cut(s) | 590, 601, 1140 |
| HaeIII | GGCC | 4 cut(s) | 147, 925, 1026, 1125 |
| HapII | CCGG | 1 cut(s) | 461 |
| HgaI | GACGC | 1 cut(s) | 1214 |
| HhaI | GCGC | 4 cut(s) | 589, 600, 660, 1139 |
| Hin1I | GRCGYC | 2 cut(s) | 520, 1137 |
| Hin6I | GCGC | 4 cut(s) | 587, 598, 658, 1137 |
| HinP1I | GCGC | 4 cut(s) | 587, 598, 658, 1137 |
| HinfI | GANTC | 4 cut(s) | 53, 368, 617, 1144 |
| HpaII | CCGG | 1 cut(s) | 461 |
| HphI | GGTGA | 1 cut(s) | 1363 |
| Hpy166II | GTNNAC | 6 cut(s) | 101, 734, 1181, 1190, 1330, 1405 |
| Hpy188I | TCNGA | 3 cut(s) | 382, 542, 697 |
| Hpy188III | TCNNGA | 4 cut(s) | 511, 844, 904, 947 |
| Hpy8I | GTNNAC | 6 cut(s) | 101, 734, 1181, 1190, 1330, 1405 |
| HpyAV | CCTTC | 4 cut(s) | 631, 790, 1043, 1195 |
| HpyCH4III | ACNGT | 4 cut(s) | 37, 98, 105, 188 |
| HpyCH4IV | ACGT | 7 cut(s) | 322, 520, 1018, 1078, 1096, 1177, 1186 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 17, 251, 270, 273, 595, 1267, 1272, 1276 |
| HpyF3I | CTNAG | 4 cut(s) | 539, 882, 936, 996 |
| HpySE526I | ACGT | 7 cut(s) | 322, 520, 1018, 1078, 1096, 1177, 1186 |
| Hsp92I | GRCGYC | 2 cut(s) | 520, 1137 |
| HspAI | GCGC | 4 cut(s) | 587, 598, 658, 1137 |
| KasI | GGCGCC | 1 cut(s) | 1136 |
| Kzo9I | GATC | 5 cut(s) | 66, 463, 558, 840, 1238 |
| LguI | GCTCTTC | 1 cut(s) | 708 |
| LmnI | GCTCC | 4 cut(s) | 315, 978, 985, 1318 |
| Lsp1109I | GCAGC | 9 cut(s) | 215, 232, 235, 238, 240, 251, 260, 273, 1273 |
| LweI | GCATC | 3 cut(s) | 556, 1057, 1402 |
| MaeI | CTAG | 7 cut(s) | 77, 174, 236, 258, 590, 1170, 1283 |
| MaeII | ACGT | 7 cut(s) | 322, 520, 1018, 1078, 1096, 1177, 1186 |
| MaeIII | GTNAC | 2 cut(s) | 397, 742 |
| MalI | GATC | 5 cut(s) | 68, 465, 560, 842, 1240 |
| MbiI | CCGCTC | 1 cut(s) | 144 |
| MboI | GATC | 5 cut(s) | 66, 463, 558, 840, 1238 |
| MboII | GAAGA | 5 cut(s) | 695, 1352, 1355, 1358, 1406 |
| MfeI | CAATTG | 2 cut(s) | 278, 1196 |
| MflI | RGATCY | 1 cut(s) | 463 |
| MhlI | GDGCHC | 4 cut(s) | 1028, 1192, 1251, 1407 |
| MlsI | TGGCCA | 1 cut(s) | 1125 |
| MluCI | AATT | 7 cut(s) | 278, 691, 759, 910, 916, 955, 1196 |
| MluNI | TGGCCA | 1 cut(s) | 1125 |
| Mly113I | GGCGCC | 1 cut(s) | 1137 |
| MlyI | GAGTC | 2 cut(s) | 362, 1153 |
| MmeI | TCCRAC | 2 cut(s) | 337, 814 |
| Mox20I | TGGCCA | 1 cut(s) | 1125 |
| MroXI | GAANNNNTTC | 1 cut(s) | 702 |
| MscI | TGGCCA | 1 cut(s) | 1125 |
| MseI | TTAA | 1 cut(s) | 1365 |
| MslI | CAYNNNNRTG | 7 cut(s) | 96, 321, 512, 902, 1131, 1233, 1410 |
| Msp20I | TGGCCA | 1 cut(s) | 1125 |
| MspA1I | CMGCKG | 1 cut(s) | 273 |
| MspI | CCGG | 1 cut(s) | 461 |
| MspR9I | CCNGG | 4 cut(s) | 449, 461, 1046, 1122 |
| MunI | CAATTG | 2 cut(s) | 278, 1196 |
| MvaI | CCWGG | 3 cut(s) | 449, 1046, 1122 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 17, 251, 270, 273, 595, 1267, 1272, 1276 |
| NarI | GGCGCC | 1 cut(s) | 1137 |
| NciI | CCSGG | 1 cut(s) | 461 |
| NcoI | CCATGG | 1 cut(s) | 1126 |
| NdeI | CATATG | 1 cut(s) | 525 |
| NdeII | GATC | 5 cut(s) | 66, 463, 558, 840, 1238 |
| NheI | GCTAGC | 1 cut(s) | 76 |
| NlaIV | GGNNCC | 4 cut(s) | 453, 1026, 1138, 1159 |
| NmuCI | GTSAC | 1 cut(s) | 742 |
| NspI | RCATGY | 2 cut(s) | 378, 497 |
| OliI | CACNNNNGTG | 2 cut(s) | 96, 321 |
| PaeI | GCATGC | 1 cut(s) | 497 |
| PagI | TCATGA | 2 cut(s) | 903, 946 |
| PciSI | GCTCTTC | 1 cut(s) | 708 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1183 |
| PdmI | GAANNNNTTC | 1 cut(s) | 702 |
| PfeI | GAWTC | 2 cut(s) | 53, 617 |
| Pfl23II | CGTACG | 1 cut(s) | 475 |
| PleI | GAGTC | 2 cut(s) | 362, 1152 |
| PluTI | GGCGCC | 1 cut(s) | 1140 |
| PmaCI | CACGTG | 3 cut(s) | 323, 1079, 1097 |
| PmlI | CACGTG | 3 cut(s) | 323, 1079, 1097 |
| PpsI | GAGTC | 2 cut(s) | 362, 1152 |
| Ppu21I | YACGTR | 3 cut(s) | 323, 1079, 1097 |
| PsiI | TTATAA | 2 cut(s) | 915, 960 |
| Psp6I | CCWGG | 3 cut(s) | 447, 1044, 1120 |
| PspCI | CACGTG | 3 cut(s) | 323, 1079, 1097 |
| PspEI | GGTNACC | 1 cut(s) | 397 |
| PspGI | CCWGG | 3 cut(s) | 447, 1044, 1120 |
| PspLI | CGTACG | 1 cut(s) | 475 |
| PspN4I | GGNNCC | 4 cut(s) | 453, 1026, 1138, 1159 |
| PspOMI | GGGCCC | 1 cut(s) | 1024 |
| PspPI | GGNCC | 4 cut(s) | 146, 924, 1024, 1025 |
| PstI | CTGCAG | 4 cut(s) | 169, 205, 230, 291 |
| PsuI | RGATCY | 1 cut(s) | 463 |
| RsaI | GTAC | 3 cut(s) | 102, 477, 820 |
| RsaNI | GTAC | 3 cut(s) | 101, 476, 819 |
| RseI | CAYNNNNRTG | 7 cut(s) | 96, 321, 512, 902, 1131, 1233, 1410 |
| SapI | GCTCTTC | 1 cut(s) | 708 |
| SaqAI | TTAA | 1 cut(s) | 1365 |
| Sau3AI | GATC | 5 cut(s) | 66, 463, 558, 840, 1238 |
| Sau96I | GGNCC | 4 cut(s) | 146, 924, 1024, 1025 |
| SchI | GAGTC | 2 cut(s) | 362, 1153 |
| ScrFI | CCNGG | 4 cut(s) | 449, 461, 1046, 1122 |
| SduI | GDGCHC | 4 cut(s) | 1028, 1192, 1251, 1407 |
| SfaNI | GCATC | 3 cut(s) | 556, 1057, 1402 |
| SfcI | CTRYAG | 6 cut(s) | 57, 165, 201, 226, 287, 876 |
| SfoI | GGCGCC | 1 cut(s) | 1138 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 96, 321, 512, 902, 1131, 1233, 1410 |
| SmlI | CTYRAG | 3 cut(s) | 329, 498, 1312 |
| SmoI | CTYRAG | 3 cut(s) | 329, 498, 1312 |
| SpeI | ACTAGT | 1 cut(s) | 257 |
| SphI | GCATGC | 1 cut(s) | 497 |
| Sse9I | AATT | 7 cut(s) | 278, 691, 759, 910, 916, 955, 1196 |
| SsiI | CCGC | 8 cut(s) | 144, 178, 208, 271, 1036, 1057, 1070, 1416 |
| SspDI | GGCGCC | 1 cut(s) | 1136 |
| SspI | AATATT | 1 cut(s) | 435 |
| SspMI | CTAG | 7 cut(s) | 77, 174, 236, 258, 590, 1170, 1283 |
| StyD4I | CCNGG | 4 cut(s) | 447, 459, 1044, 1120 |
| StyI | CCWWGG | 2 cut(s) | 1126, 1169 |
| TaaI | ACNGT | 4 cut(s) | 37, 98, 105, 188 |
| TaiI | ACGT | 7 cut(s) | 325, 523, 1021, 1081, 1099, 1180, 1189 |
| TaqI | TCGA | 3 cut(s) | 126, 351, 557 |
| TasI | AATT | 7 cut(s) | 278, 691, 759, 910, 916, 955, 1196 |
| TatI | WGTACW | 1 cut(s) | 100 |
| TauI | GCSGC | 3 cut(s) | 147, 180, 1418 |
| TfiI | GAWTC | 2 cut(s) | 53, 617 |
| Tru1I | TTAA | 1 cut(s) | 1365 |
| Tru9I | TTAA | 1 cut(s) | 1365 |
| TscAI | CASTG | 1 cut(s) | 1256 |
| TseFI | GTSAC | 1 cut(s) | 742 |
| TseI | GCWGC | 9 cut(s) | 203, 228, 245, 248, 251, 264, 273, 286, 1286 |
| Tsp45I | GTSAC | 1 cut(s) | 742 |
| TspDTI | ATGAA | 4 cut(s) | 230, 842, 935, 1413 |
| TspGWI | ACGGA | 1 cut(s) | 853 |
| TspRI | CASTG | 1 cut(s) | 1256 |
| VneI | GTGCAC | 2 cut(s) | 1188, 1403 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 972 |
| XceI | RCATGY | 2 cut(s) | 378, 497 |
| XmaJI | CCTAGG | 1 cut(s) | 1169 |
| XmiI | GTMKAC | 2 cut(s) | 1180, 1329 |
| XmnI | GAANNNNTTC | 1 cut(s) | 702 |
| XspI | CTAG | 7 cut(s) | 77, 174, 236, 258, 590, 1170, 1283 |
| ZraI | GACGTC | 1 cut(s) | 521 |